LOCUS       DAONHK010000001        40141 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_757_length_40141_cov_5.183431, whole genome shotgun sequence.
ACCESSION   DAONHK010000001 DAONHK010000000
VERSION     DAONHK010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 40141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 40141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40141
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_757_length_40141_cov_5.183431"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1197)
                     /locus_tag="PLM92_00005"
     CDS             complement(<1..1197)
                     /locus_tag="PLM92_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003406540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HPR23560.1"
                     /translation="MLREALPKVNMPLPGPKAQEIIKRREAAMPNAIKCTYPLVIKRG
                     EGAMIEDVDGNILLDWIGGVGVLNIGYSQPELIDAVKGQSEKFFHAMMNITTHEPYIT
                     LAEKINSIAPVKGDKKKTMFVNSGAEADENAVKIARSYTGRPNIIVFSGAFHGRTQLT
                     MTMTGKKAYAIGMGPFPDGIYRVDFPNMYRRPDDSMTDEEAIKYYVNKLEMAFIEAAP
                     ADSVAAILFEPVQGEGGFIPAPMEWVKAVRQICDKHGIMMITDEVQTGFARSGRMFVS
                     DYYKEAGFAPDIMVIAKSIAGGMPLSAVVTSTEIMDDVTPGTIGGTYCGNPLSCASSL
                     AVINVMEKEDYPAKARKIAEKCMAKFNEWKEKYEVVGDVRGTGAMMGVEFVKSRATKE
                     PNAEIVG"
     gene            complement(1225..3036)
                     /locus_tag="PLM92_00010"
     CDS             complement(1225..3036)
                     /locus_tag="PLM92_00010"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HPR23561.1"
                     /translation="MSLLQDISISVQQVAEAISIAVGVEVEIVDSSLTIIGGTGLYSD
                     MIGQKEEAGKLDGNFLYARVLRNGVTQYVEDTRSERFYDEQKDRAKTVGELAEICTPI
                     KRNDEIIGIIGLVAFNEEQRGILLDKDRSMVTFVEKMADLLAAKAAQQEDLQMTELSR
                     KEMITVLETAHEGIFAIDKGGYIRHCNSTAAELLKTTKNDIIGEHINKYMLSSPALDV
                     FKTGKGYTENEELYRGERGGLHAIVTAKPYFSGNEIEGVVLSFRDIEEAEKLAYHINI
                     RKVKNTFDDIIGKSGSITRAKNQALITARGDSTVLITGESGTGKEMFAKAIHYSGPRA
                     GQPFVTVNCGAIPENLLESELFGYEKGAFTGASEKGKIGKFEMADGGTIFLDEIGDMP
                     SHLQVKILHVLQNMRFERVGGNKSIMVNVRVIAATNRNLEEMIKEGTFREDLFYRLSV
                     IPIDIPPLRERKEDVELFMDHFLKKYNKYMNRKLRGFTDEVRDLYMTYDWPGNVRELE
                     NAIEYSTNMAFGEIVGMDDVPMRLQRSEENIISVSDSDLPLADQIKEYEREIILKKFR
                     KHSAGGDGKETVAKELGLSRATLYRKLAELDINKRLL"
     gene            complement(3130..4827)
                     /locus_tag="PLM92_00015"
     CDS             complement(3130..4827)
                     /locus_tag="PLM92_00015"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006001912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPR23562.1"
                     /translation="MHIKFCGAALGVTGSCHLLIAGGHKILLDCGQFQGGKQQEAQNF
                     DEFPFEPKEIECVLLSHAHIDHCGRLPLLVKRGFKGKIFCTDATADLLPVMLKDSAYI
                     HEKEAEWQNRKNERAGRSLVEPLYTIKDSEEALKLTFPVLYDQLFKINDEMEAVFNEA
                     GHILGSAITEIWVKEKTGENGQNEQQENGKEKISKIVFSGDLGVMDRPILRNPKIIKK
                     ADYLIMEATYGNRLHPENSASIDQLLDIVIKTTDRGGTVVIPSFAVGRTQELIFEFNR
                     FYEENEQYKEKLDKINVYIDSPMATTATEVFKKNAQVFDEETKEYILKGDNPLDFKNL
                     KFTRTSDDSRALNMNREPKIIISASGMCEAGRIRHHLKHNLWDPKSSVVFVGYQAEGT
                     LGRNLLNGLKDVTLFGEEIHVNAEIYDLEGFSAHADREGLLEWISGFIKEPKQIFLVH
                     GETDAKADLAAAIKEKLGYDIVTVEGISEFELDHGEMLSKEEALADALDEETMETIRK
                     RIADVHEYIEKSLYNTSLSVGSEISPKKLVDINNIVLQLEKASLSLGSVLTDRDEQN"
     gene            complement(4850..5788)
                     /gene="cysK"
                     /locus_tag="PLM92_00020"
     CDS             complement(4850..5788)
                     /gene="cysK"
                     /locus_tag="PLM92_00020"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007048842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HPR23563.1"
                     /translation="MLYKNIIKTIGNTPLVYLSRYEFSKDDGTISSGNIYAKIEGKNP
                     GGSIKDRTALYMIEDAEKKGLLKKGSVIIEPTSGNTGIGLALVAGAKGYKTILVMPDS
                     MSRERRDLLSAYGAELYLTEGTRGMRGAIEKAENLTSETRGAFMPGQFTNMANPKAHF
                     ETTGPEIWKDSGGKVDIFVSGVGTGGTITGAGRFLKNKNPAVKIVAVEPKKSNVLTGG
                     TSGPHGIQGIGAGFIPDILDRSVISEVMKVSDEDAFDTAKKLAKTEGILAGISSGAAL
                     YAASVLASKKENKDKNIIVILPDTGERYLSSGLFDL"
     gene            complement(5959..7251)
                     /gene="preA"
                     /locus_tag="PLM92_00025"
     CDS             complement(5959..7251)
                     /gene="preA"
                     /locus_tag="PLM92_00025"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HPR23564.1"
                     /translation="MAVKKDLSIKYLGVDCENPFFLSSSPVGGCYDMVAKCFETGWGG
                     VFFKTVGIFVADECSPRFDQTNKEGIPWLGFKNMEQISDKPTEKNFEYIYKLKKDYPD
                     KVVVASIMGSSDKEWRDLAKMADQAGADLIEGNFSCPQMTSHDMGSDVGTNVKLVEAY
                     SKAVSETTNIPFIAKMTPNCKNMEEPARAAIKGGAAAVSAINTIKSITNVDFENNTGM
                     PVVNGKSSISGYSGAAVRPIALRFCAQVMQDPEMNKLLKANKKDFGYGHEMSGIGGIE
                     TWKDAAEFLSVGCRNVQVTTAIMQYGYRIVEDMISGMMHFMEEKGYNKLDDFIGCVLP
                     NLIPAEELNRDFKVLPKFDNKKCVGCGRCYISCYDAAHQAIDWDEKKRRPVLLEDDCV
                     GCQLCLNVCPVQDCITTGEIKWKKGRKKVKVATKQHFD"
     gene            complement(7253..8518)
                     /locus_tag="PLM92_00030"
     CDS             complement(7253..8518)
                     /locus_tag="PLM92_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPR23565.1"
                     /translation="MSKVEKAKYTAEAQAGFNHRTAMEEAARCLLCHDAPCSEGCPAG
                     TDPGKFIRSIRFRNAKGAAETIRENNILGGSCARICPYDRLCEEACSRCGIDKPIQIG
                     KLQRYAIEQEKIFKMKTLKAPAKKMKEKVACVGAGPASLACAAELAKAGYKVTVFDRE
                     EKAGGVMTYGIVPTRLPQKVVDHDIAQVKGLGVKFVLGKKVKPAELEAFDAVFVGVGL
                     WGAKVPPIKGTNLKGVYAAADFLKQARTKKTFKPGKKVVVVGGGDVAVDCAVTAKLLG
                     AEDVRIVYRRTLEEAPGNMTEFQYALSLGIGITTNMAPSSVLGTNKVEAVKFKGRPKV
                     TESSELKLEANTVVFAIGQGAEDFSELAPVKLTEKGMIKATAKGKTSSKKYFAGGDIV
                     NGGKTVVEAVASGKEAAASIIEFLQKGVK"
     gene            complement(8745..10028)
                     /locus_tag="PLM92_00035"
     CDS             complement(8745..10028)
                     /locus_tag="PLM92_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004604200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HPR23566.1"
                     /translation="MAYKCDLNRMSDKIKTFSKFGDAGHGGITRYSLSPEAHMAREEF
                     VKRMEAIGAKIECDDVACMYATIEGSDPNAKRIVLGSHVDSVKNGGNYDGILGVMTGM
                     EVMETIVAEKIPHKHPITTMIWTNEEGSLYPPAMMVSGIICNDYIPEELRVNFKYENV
                     MKSTPMDESEFANFGEALAATKFKGPKENRISPDKYCAMFETHLEQGPILEEAGNEVG
                     AVQLVMGMFNYRVRFHGFTAHAGTFPMGKRHDALFAASTFLCEMHKRFDELNKGQEPE
                     FVYTTGEIYVHPCIHTCIPDEAEFSLDVRHPDPELRKKVMGILKDMAKEEWCKCACEI
                     EEQWVRDTVYFDKNLVRFVEESMEESGMKWQKILSGAGHDAQFCAYMLPTTMIFTRTK
                     DGLSHCEPEFVSDEDCTAGATAALGAVLKADQFYE"
     gene            complement(10212..10694)
                     /locus_tag="PLM92_00040"
     CDS             complement(10212..10694)
                     /locus_tag="PLM92_00040"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007050942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPR23567.1"
                     /translation="MKKVIIEMENGEMMKGELYENTAPKTVANFEALANKGFYDGLLF
                     HRVIPGFMIQGGCPMGDGTGGPGHTIEGEFSDNGIENDILHERGVLSMARAANPNSAG
                     SQFFIMVDDAPYLDGQYAAFGRITEGMEEADRIVSTKRDMRDKPKKPQKIKKLTVLNE
                     "
     gene            complement(10699..11154)
                     /locus_tag="PLM92_00045"
     CDS             complement(10699..11154)
                     /locus_tag="PLM92_00045"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HPR23568.1"
                     /translation="MWIGGARTVILDEKKRVLMVRQHHEGKDIWMLPGGGIEENEDSR
                     QAAAREIKEETGFDVEIGMLIWHVEQVREGKEQRFVNFFMGEIKGGSLKKGNDPEREE
                     NEQVIREVRFMGREEMERTKNLYPEYLRDELWEFIESGTEKQEVFKIRR"
     gene            complement(11145..11684)
                     /locus_tag="PLM92_00050"
     CDS             complement(11145..11684)
                     /locus_tag="PLM92_00050"
                     /inference="COORDINATES: protein motif:HMM:NF014471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HPR23569.1"
                     /translation="MKFIHLFMVFFFIGAFSFGGGIAMLPLIFQMVQKYHMLSPEKFS
                     DLIAVSQAVPGAVAPNAATYTGIEYAGIFGGILAAFSIALPSFVIVLLVMKFMGRYRK
                     SRIVEGIFEGMRPTVIGLISAAVVFIAGTSIYRPAPFSVELIPLGIFMVTLILSGKFK
                     INPIIIIIVMGTAGAFLCG"
     gene            complement(11681..12259)
                     /locus_tag="PLM92_00055"
     CDS             complement(11681..12259)
                     /locus_tag="PLM92_00055"
                     /inference="COORDINATES: protein motif:HMM:NF014471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HPR23570.1"
                     /translation="MTKKDKAKELFVLFLAFFKTGFFTVGGGYAMIPIIQRVIVEEKK
                     WLTETEMIDCLAVCQALPGAIAVNAATYIGNKRNGITGAAVATIGMLMPSVICITAVV
                     LLLGALGQSTYISGAFTAIKASACGLITVTVFDMAKKVLRKPFHWTVALLVFIAIGIF
                     EIMAVIPIILGGTAGIVYMAVKRKKTEAGGHQ"
     gene            complement(12262..13986)
                     /locus_tag="PLM92_00060"
     CDS             complement(12262..13986)
                     /locus_tag="PLM92_00060"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005998645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HPR23571.1"
                     /translation="MRLSKMHFKTLREVPSEAEIPSHILMLRAGIIKKLVSGVYGFMP
                     MGWRSVRKIEEIIREEMDGAGGQEIHMSAVQPAELWEESGRWFAYGPELWRLKDRNGR
                     EFCLGPTHEEIFTDIVRNDISSYRQLPENLYQIQTKYRDEARPRFGLMRSREFIMKDA
                     YSFDRDEEGLNRSYEDMYAAYEKIFDRCGLKYRAVDADTGAMGGSNSHEFTALSDVGE
                     SQIAYCENCHMAATVERAETPDAKHDDEEMKPLEEVGTPDMKTIEDVAAYLGLKKDQT
                     IKALLFVTYDNDEKESGYVAAFIRGDRELNMVKLVNALGIPEHSIDFADEGKMGPATG
                     CVGGFTGPVGLHDLTIVVDSELPGQKNLCAGACKKDAHLKNVNYGRDYEADIVADIKL
                     IRENDPCPSCGEPVKIARGIEVGQVFKLGTKYSESMGAYYKDENQKSRPIVMGCYGIG
                     VTRTLAAIVEQHHDDDGILWPLSVAPYQVIIIIVKTSDETQMKEGEKLYSELKKAGAD
                     VLLDDRDERPGVKFKDADLIGIPVQITVGRGAAEGKAEYKLRCGSKKEELTFDEAIKR
                     TVGMINGE"
     gene            complement(14057..14548)
                     /gene="ispF"
                     /locus_tag="PLM92_00065"
     CDS             complement(14057..14548)
                     /gene="ispF"
                     /locus_tag="PLM92_00065"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014182769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HPR23572.1"
                     /translation="MKDIREIRTGIGFDVHRLEYGRKLVLGGITIPFEKGLLGHSDAD
                     VLVHALMDALLGAASMGDIGMHFPDTDDRYRDISSIKLLRHVTGIIREKGYHICNADI
                     IVMAQQPKIAKFTREIKENLAEAMGIEEDFVNIKGTTTEKMGTIGKGEGIAAEAACLL
                     IKY"
     gene            complement(14545..15651)
                     /locus_tag="PLM92_00070"
     CDS             complement(14545..15651)
                     /locus_tag="PLM92_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HPR23573.1"
                     /translation="MLKKLMRLVFTLVGGIIGYGAFLLTKYLLTISDIGPGRGFTQWQ
                     QIGAAIVFVIIFALLFFRLTPSVKRQSQKMARNIESDLQSYSTNELIAGITGLVTGLL
                     IAFLISQIYAGIEVDYLPTVLTVLTYLLLGYLGVVVATKKGKEGLAALMSARKATASS
                     GKSKKQSDATPKILDTSVIIDGRISDIMKTGFIEGNIVIPEFVLVELRHIADSSDSLK
                     RNRGRRGLDVLNKIQSEYGVEIYNTTGEKSLDEIPEVDVKLLKLAQIMNGKVVTNDFN
                     LNKVASIKGVEVLNINELANTLKPVVLPGEDMSLFLVKEGKENNQAVAYLDDGTMIVV
                     EDGRRHIGKTIEVTVTSVLQTSAGRMIFGKPARK"
     gene            complement(15718..16191)
                     /locus_tag="PLM92_00075"
     CDS             complement(15718..16191)
                     /locus_tag="PLM92_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003545204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HPR23574.1"
                     /translation="MYSIGDKIVYPMHGAGIIEQIEEKVILGEKRKYYVLNVPCGDMK
                     IMIPVEKSKDIGVRDVISSKDVDTVLEVLNAPSTEMSDNWNRRYRENMEKLKTGDICE
                     VAEVVRNLLRIEREKNLSAGEKKMLANARQILVSEIILARDMETAKAEELVIDSI"
     gene            16373..17044
                     /locus_tag="PLM92_00080"
     CDS             16373..17044
                     /locus_tag="PLM92_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019662424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPR23575.1"
                     /translation="MPKLLVVDDEQKIREVIREYSEFNGYEITEAEDGMTAVGLCKLN
                     DYDLIIMDIMMPKLDGFSACKEIKKIKDIPIIMLSARSEEYDKLFGFELGIDDYVIKP
                     FSPKELMARVNAVLARRSKPSKAAAEILEFDGLVINIPGRIVTVDGEKIELTPKEYDL
                     LFYLVNNKNIALSRDKLLSDIWGYDFFGDDRTIDTHIKNLRNNLGPYRDYIVTLRGVG
                     YKFEV"
     gene            17034..18446
                     /locus_tag="PLM92_00085"
     CDS             17034..18446
                     /locus_tag="PLM92_00085"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPR23576.1"
                     /translation="MKFKFDYKSIKARVWVYFLCFTVIVLMLVWFLQILFINNYYEEM
                     KVRETKRIASNITSDYTDGNINDIVSEVEKLSENDDMFIRIESLKSGIVFSPSVDDRG
                     YENEIETVKGKLITSGKNNISIIISSPYNSRNVLVYAGFLDPAEENILYVFSPLYPVK
                     STVSILQNQLVYITIIALILATVIAYILSIRITKPIRSIIRSAGKLSAGQYGITFRTE
                     KTYSEIKDLANSLNKASYELEKSINLQKDLMANVSHDLRTPLTMIKSYAEMIRDLSGN
                     DPKKRDQHLKVIIEEADRLNALVGDMLTLSAMQSGKISLSKSIFNIKEAAENVLLPYR
                     LLEEKEGYRIKFICRQNIYVNGDLERMKQVISNLLTNAVKYCGADKTITLSIKRTGKK
                     MRCEVKDNGMGIKPEELPHIWERYYKASSNHVRATTGSGLGLSIVKEILSLHRADFGA
                     NSKPGHGSSFWFELDTVKKR"
     gene            complement(18433..19809)
                     /gene="radA"
                     /locus_tag="PLM92_00090"
     CDS             complement(18433..19809)
                     /gene="radA"
                     /locus_tag="PLM92_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017417155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HPR23577.1"
                     /translation="MAKKQKTVFVCQECGYESPKWMGQCICGAWNTMTEEKVLSFDGI
                     DKRGIGKAKKGKRKPAALKDIRSSEGPERIDTGIGELNRVLGGGMVKGSLTLISGEPG
                     IGKSTVIMQAALKIAEKYGRVLYVSGEESEEQIKMRADRICGSICKDLFIMGETNIEN
                     ICASVEELGPVFVVIDSIQTMYTSFMESSPGSVSQVRACANELMRIGKVTGTPVFIVA
                     HVTKSGELAGPKIIEHLVDCVLDLTGERNRELRIIRAQKNRFGTTSEIGAFEMTGEGL
                     VEIENLSKTFLEGTEEGSDGSVVTAVHEGTRPLLLEVQALAAPVSVGFARRSSIGVDL
                     SRLNMIIAVLEKKAGLPLMNQDIYVNIVGGLRPEGTSADLAVALAIYSACTAKPCNEG
                     TIVIGEVGLTGNLRAVQNAEKIIKEAERMGFKKVILPKRNASKLAEERLKIELKGVSS
                     ITEAISVF"
     gene            complement(19864..20799)
                     /locus_tag="PLM92_00095"
     CDS             complement(19864..20799)
                     /locus_tag="PLM92_00095"
                     /inference="COORDINATES: protein motif:HMM:NF018437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HPR23578.1"
                     /translation="MRKKLISVIMIFAMAMALAPKGAMAEVTAAPPEVSAATVSHSQI
                     SISWQAVENSQGYEIYRYNSVTGKYEIIRSVRGETLCFIDSGLKINKTYKYYVNALLL
                     AEDGTTIIKSGISRICSAKTIPAAPINVKYSVTSRKHIKITWKKVSGVSGYYVYRASK
                     GGKYKKIKTITKAGTTSFSNSSITANKTYAYKIKAYKKSGKKKISGRSSASIDANNGM
                     AYKKKLKVKAYAYTGKGTCANGKKARIGRIAVDKKVIPLSTWLYVPGYGLCQACDTGV
                     KGKKIDVYLSSEKECRKWGIKKKTVYIIGKKLPLV"
     gene            complement(20945..21781)
                     /locus_tag="PLM92_00100"
     CDS             complement(20945..21781)
                     /locus_tag="PLM92_00100"
                     /inference="COORDINATES: protein motif:HMM:NF024875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S double cluster binding domain-containing
                     protein"
                     /protein_id="HPR23579.1"
                     /translation="MEIKQIVYNIFDSLEKNNFATLGRPEDPMWERPVIGVAAGNDPY
                     YDFLKDHIGSFHWNPQEVFRLKYKNVPKAEKLRVVSMVFPQTKTTKKHQGKATVFPSD
                     NWVVSRGEWEPLMQEFSGKLVKRLEDEGLRCVSVDLQKEFKRMHSENLGIASKWSHRH
                     SAFAAGLGTFSLSDGFISEKGKAIRISSLIIEADLMITPRGDRGPYDWCLYYQDGSCG
                     KCIKRCPVEAISGKGHEKYTCEAYEDEAVSKYWPKHIERGDYIFGCGLCQTDIPCESK
                     KP"
     gene            complement(21792..22340)
                     /locus_tag="PLM92_00105"
     CDS             complement(21792..22340)
                     /locus_tag="PLM92_00105"
                     /inference="COORDINATES: protein motif:HMM:TIGR01383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HPR23580.1"
                     /translation="MVFIHLAEGFEEIEAVTIMDILKRAGIEGAFVSVPGGQKDMTVC
                     GGHGIKIKADMDFADADYEKCEMIVLPGGMTGTLNLKKHDGLCSRIREFALKDKWLSA
                     ICAAPMVFAGLSVLEEKEAVIYPGLEKKLKGARPSEKPVVKDGKVITGRSPGTAMLFA
                     LEIVKALKGQDIMEKVRTGLAM"
     gene            complement(22423..24366)
                     /locus_tag="PLM92_00110"
     CDS             complement(22423..24366)
                     /locus_tag="PLM92_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019034689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HPR23581.1"
                     /translation="MKGYTSETIRNVALVGHGGSGKTNFLEAALLATGVISRLGKVED
                     GNTVSDWDKMEIEKGYSISTSLVPVEFNKVKMNFVDTPGFFDFAGEVSSTLRAVEAAA
                     ILVDASSGIEVGTEKAWDSCKKLGVPRFFLINKIDKENVDTDQLIADLKSKFGTSVVT
                     LDDKDALNEAVAETDEALMEKFFSGEEFTEEEFNKGLSGGIGEGDISPVMLCSAHEGT
                     GVKEALQTFIDLVPKATDHAPYPAKDGSEKDIEVKCDPSGKPAILIFKTVADQVLGKI
                     SFGKIISGKLKGGQEIYNSVSEKSEKLGTMFFLRGKTRVDTAEAEAGDIIAIGKLQNT
                     RTGDTLCDKNSIVVFPPIDFPQPTFFIAVEPKSKGDEEKVGTGLNRLMEEDPSFILQR
                     NTETQQTLLGGQGEQQLNVITAKLKDKFGVDVDRVNQKIAYRETIKGSSDVQGKHKKQ
                     TGGAGQYGDVHIRFSPSQQDNLEFTESLFGGSIPKNYVPAVEKGLKESMDKGPLAGCK
                     VVGIKADLYDGSYHDVDSNEMAFKIAASLAFKKGIVDANPCLLEPVMHIEITVPDEYM
                     GDIMGDMNKRRGKILGSEPLAGGGQKIMAEAPQAELFDYAVTVRSMTQARGNFIMNFE
                     RYEPVPGHLAEKIIAEHKKEEEK"
     gene            complement(24481..24948)
                     /locus_tag="PLM92_00115"
     CDS             complement(24481..24948)
                     /locus_tag="PLM92_00115"
                     /inference="COORDINATES: protein motif:HMM:NF019292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HPR23582.1"
                     /translation="MSESWSYILNEMLTGSLSTVLNLLKVLIPLMIIIELLVQYNILE
                     KISKRLEFMGKPMGMSRDAIFPLLVGTVMGVTYGAGTLMELNERKPLSKKDFALIGVF
                     MYLCHGIIETGFLFGINGANVFVVVVVRLLIAFFVTCIAARLPYFRKMEHEKV"
     gene            complement(24945..25379)
                     /locus_tag="PLM92_00120"
     CDS             complement(24945..25379)
                     /locus_tag="PLM92_00120"
                     /inference="COORDINATES: protein motif:HMM:NF019292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HPR23583.1"
                     /translation="MKKFFNAIKNGTFKGLKTGLMLLKIMLPIYLLVVLLKYSPVMPW
                     LQEICQPVMKLFNLPGDAAVPIITGIFTDEYGCVAAMGGFEFSSAAITTIAMVNLMAH
                     SIPVETAIAHKIGFSSGKLAIYRVVAAVLVGIFIGWLGGVFL"
     gene            complement(25414..26808)
                     /gene="asnS"
                     /locus_tag="PLM92_00125"
     CDS             complement(25414..26808)
                     /gene="asnS"
                     /locus_tag="PLM92_00125"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003375192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HPR23584.1"
                     /translation="MKEAQIKKLYRDPKEYEGQDISVRGWIRTNRGSNKFGFIELNDG
                     SFFKSVQVVYEAEVINNFDEISKVPISAALKVRGEFVLTPDSKQPFEIKAFEIEVLAG
                     SDADYPLQKKRHSLEYLREIAHLRPRSNTFSAVFRIRSITAYAIHRFFQENDFVYVHT
                     PIITGSDAEGAGEMFQVTTFDMEKPPRDEKGRIDYSEDFFGKRAGLTVSGQLEAEIFA
                     LAFRNVYTFGPTFRAENSNTARHASEFWMIEPEMAFCDLEGNMEVAEAMVKYIIRYVM
                     ENAPEEMEFFNGFIDKGLIKRLENIVESDFARVTYTEAVDLLKKSHKDFQYPVEWGID
                     LQTEHERYITEEIYKKPVFVTDYPKEIKAFYMRQNDDGKTVAACDLLVPEIGEIIGGS
                     QREERYDTLKERMDEAGLDEKDYWWYMELRKYGGVKHSGYGLGFERMIMYLTGISNIR
                     DVLPFPRTPRTAEF"
     gene            complement(26808..28331)
                     /gene="lysS"
                     /locus_tag="PLM92_00130"
     CDS             complement(26808..28331)
                     /gene="lysS"
                     /locus_tag="PLM92_00130"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005837857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HPR23585.1"
                     /translation="MNDNKKVQDTVDRENESDLNEQRVIRREKLKALQEAGRNPFLIE
                     KWDQNAYSQDIKDDFDRMEGKDVSVSGRIMAKRVMGKASFTDIQDYKGRIQLHIKRDV
                     LGEEEYKWFKTYDIGDIIGVEGKVFRTQRGEVTIEAQRVVLLSKSLQVLPEKWHGLKD
                     TDLRYRKRYVDLIVNPEVKELFIKRASIIKEIKRVLEEDFGYLEVDTPILTTIAGGAN
                     ARPFETYHNTLDLEMKLRISNELYLKRLTVGGLDRVYEMGKMFRNEGMDKNHNPEFTS
                     MECYMAYGNMEDVMEVTEQVVSKAALAATGSMKITYQGKRFDLTPPWKVLKMEDAVKE
                     ITGVDFSKIDTDEEARKVAKKFGLEGAEKMTRGLVIAELFEEQCEDVPGYLDGPVFVT
                     GHPVEISPLSKRDPEDPRITRRFEAYINGWEIANAFSELNDPIDQYQRFKDQQEQLES
                     GDDEAHPMDQDFVEALEVGLPPTGGLGIGIDRVIMLVTDAQSIRDIILFPTMKPQEK"
     gene            complement(28336..28809)
                     /gene="greA"
                     /locus_tag="PLM92_00135"
     CDS             complement(28336..28809)
                     /gene="greA"
                     /locus_tag="PLM92_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005998873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HPR23586.1"
                     /translation="MTEEVLLTKEGYDKISAEYDELVSVKRAEVAERIKEAREFGDIS
                     ENAEYDAAKNEQAELEERITKLENMMRKAKIIEEGKSPKGKVNIGSKVTVKDTKTRKI
                     QDFIIVGATEADPFEGKISNESKVGEGLIGAKVGDKVDIEAPKGRIRYEVISINR"
     gene            complement(28894..29979)
                     /locus_tag="PLM92_00140"
     CDS             complement(28894..29979)
                     /locus_tag="PLM92_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/ornithine racemase family PLP-dependent
                     enzyme"
                     /protein_id="HPR23587.1"
                     /translation="MMYPRVEIDLKKLRHNIDEIVVRCRELNIEVAGVVKGFTGIPEC
                     TRQFADSECRFIATSRLEQVEDAKKHGITKEFMLIRVPMITEAAEAVRLTDISLHSEI
                     EVLKAFNEQALMQNKKHRIILMADLGDLREGFWDTEEMVSVALMVENSFEGLELSGVG
                     TNLGCYGSVSATPEKLEELVNIAESIESRIGRRLDFISGGATTSLPRVFEKNMPERIN
                     LLRVGEGIILAKDLRDLWGYDMSFMYQDVFTLKAEVIEVKDKPSHPVGEIMFDAFGMK
                     QEYEDRGIRRRALLALGKVDYAFPDMIYPRDKGIEILGASSDHTIADIQDAERDIKVG
                     DIMEFDLCYATIVYATNSPNVKITFAN"
     gene            complement(29976..31796)
                     /locus_tag="PLM92_00145"
     CDS             complement(29976..31796)
                     /locus_tag="PLM92_00145"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPR23588.1"
                     /translation="MLSKEEYKVLKERDMQRAVEIAEKIKADPDPLVKYRDSRPITDI
                     KHMIETSASDEWYGDHVAFYQKEKKGGDYKKITYKETMADINALGTALINEGLKDRRI
                     AIIGENCYWWAVSYLAVLCGTGIVVPLDKELNAKELEQLVKRSEVSCVFFTEKYKDIF
                     TKIKGEGTSSLEMLVNMNADEEKDGVRSWKGLVEKGRELLKEGNREFLDAQIIRDEMS
                     VLLFTSGTTGLSKGVMLSHGNLVEDLMVSPTVLKVNDWDIFFSVLPIHHTYECTCGFL
                     MPLYKGAAIAYCEGLKQIVKNLSEVRPTMFLGVPVLFENLYKKIWQNVRKKGKEKTLK
                     KVIKINRKTKKIGIDLGKIFFKDILAVFGGRMRMMIVGGAAINPEVLEGIQDFGIQAL
                     QGYGLTECAPMGALNPDTAPKAASVGRKFPTFGIKIEDINEEGIGEICLRGGNVMLGY
                     YEMPEATAEVIKDGWFYTGDLGYLDDEGYLYITGRKKNVIITKNGKNVYPEELEYYLS
                     NVPFIEESMVFGEDTSDGRDTSIVAAVKLDDYEVEELFGKGITDKELEDKVWEEVDKI
                     NANAPFFKKIKKVIIRKEEFEKNTSKKIKRFVESNRKQEA"
     gene            complement(31790..33544)
                     /locus_tag="PLM92_00150"
     CDS             complement(31790..33544)
                     /locus_tag="PLM92_00150"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPR23589.1"
                     /translation="MNSRKYKDKFYKVREIRDLRELIKGSVEKRKAYPAFLVKDTPGG
                     EYKPISYSKFDADIDSLGTSFVDMGLKDSKIAIIGENSYEWVVAYLAVTNGTGVVVPI
                     DRELHPKEIAHLIQRSGANVIVHSRKMGKAVDEVLAYVRNVEYVIDMTAEEDTDDRLS
                     MMKLIEKGSRLMAAGDRRFTNAEIDPDVMCALLFTSGTTGLAKGVMLSHKNIASNVYN
                     MSQYVDIIEDGVGLSVLPMHHTYELTCHIFTGLYQAITLAICEGLKHIQKNMVESRAT
                     VMLSVPLIFETMHKKIWKKLEANGSAGRARRMIIISRKLKLYNHDKIIKKIFKELHEQ
                     TGGGMVQFIAGGAAIDPRVIEDYEAMGIPMIQGYGMTENSPIIAVNRDCCSKASSVGQ
                     PMPNTKVKIIDPDENGMGEIICQGPSVMLGYYNDPEATKETIIDGWLHTGDYGYMDEE
                     GYLYISGRKKNVIVTKNGKNVFPEEVEFCLTESPYIQEVLVHGIEDERTGDTIIKAEI
                     FPDYEIIAEEKGDTNEDGLRELLKSVIDDINEEMPLYKRVRRFGIRKEEFSKTTTRKI
                     KRYNAENKSSEGQEDVKC"
     gene            33680..35251
                     /locus_tag="PLM92_00155"
     CDS             33680..35251
                     /locus_tag="PLM92_00155"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin like domain-containing protein"
                     /protein_id="HPR23590.1"
                     /translation="MKKSIVIFIIALFLGILSIPSLAVAKTPDLTYWSELDLPFLEVN
                     KKLPAEYDLRDKNLVTSCKDQGWHGTCWSFAAISSAETNLLMKGLAGGEKKDPGKVDL
                     SELQLARFMYDRSPDPSGGTKGDKIINSTDADYLDVGGEAYLTTFALASWMGPVKEKK
                     VETSYEDADKSTKLDKTLEYDLDLAHLRNAWWVNARDRDSVKSMLMKHGSPIICYYSG
                     YWDYSYHEVPGASSTNMTYYNPYSGEMPDHEVAVIGWDDDYPAENFYPSPGKDGAWLV
                     KNSWGSKWGNDGCFWLSYYDHGIDDYAVFLDFDKADNYQYIYQYDGGGSTAQDIAYKK
                     KAFMSNVFKSKKDETLKAVSFFATQNSKIKYFIQIYTDITDPKDPQSGRPRLSEKQTG
                     RTSYAGYYTVDLDYPVRLKEKLPFSVVIEFKTGRNENIFLPVDTDAQRYGWSEYDVTS
                     AKGQSFVSKDGEEWKDLSADGDTNIRIKAFTDDSEPLWELWIAGAIVIIAFFTFLAAL
                     IFRASRKKREERAKI"
     gene            complement(35272..35862)
                     /locus_tag="PLM92_00160"
     CDS             complement(35272..35862)
                     /locus_tag="PLM92_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004075624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPR23591.1"
                     /translation="MLSEIIRTLRENKKYTQKQLADILSVSDKTISKWETGKGLPDIT
                     LIEPLAKALNVSVAELLSGEYMINRNKSANMLKCNFYVCPVCGNVISSTGEGAFSCCG
                     ITLPPLEAETAEDGDEASLQHRMSINEIEDEYYVTLDHPMEKDHYISFFVYATFDRIQ
                     TVKLYPEQDPGFRIQRSGRGMIYAYCNKHGFFKQKI"
     gene            complement(36137..36212)
                     /locus_tag="PLM92_00165"
     tRNA            complement(36137..36212)
                     /locus_tag="PLM92_00165"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36177..36179),aa:Ala,seq:ggc)
     gene            complement(36219..36294)
                     /locus_tag="PLM92_00170"
     tRNA            complement(36219..36294)
                     /locus_tag="PLM92_00170"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36259..36261),aa:Thr,seq:cgt)
     gene            complement(36400..39711)
                     /locus_tag="PLM92_00175"
     CDS             complement(36400..39711)
                     /locus_tag="PLM92_00175"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HPR23592.1"
                     /translation="MLNVYYGREDLIKEKFIFESIKRSRQQNNREVIMIVPDQYTLQA
                     ERDAFFYLNEKILLDMEIMSFSRLATIILSETGGGRKTLIDKRGRHMLLSRILMKISN
                     ELELYKGSCSSSTFIDMTNNFISEMKQYGVSPEMLAEIKEHVDNKSFLFRKLSDIEKI
                     YTEYEKRIKGKYIDTEDQIDIFSAKIQDSKKIRKKTVWVYGFDYFTPKNLQVISKLRN
                     ASAEVNIVMTWDDRGPDKELFGITGEMIKRLRPENCFRIGREYLAKRAPVIDKLEKEV
                     FSLPAGAHDITEGIELIEAANFYTEAETAAAFVRKTVREKRISYKDISVICNDMTERG
                     SICKRVFAEYGIELFMDRKRDIMHSPIVSYIISLLNIIGGGYKTEDVFSMLKTGLAGF
                     ENNEIEELENYSHKYKVRGSAWKKEFVKGETEYGKDGIKELNDLRSRLVEPMKALGTG
                     LKEARTVNEKTALLYDFLADKSGVPEKTETLISQQKHVGHLEEAEETAQIWNVTAGLM
                     DQMVEIIGQEKISMKDYALLFEAGFSSVEIGVLPPAADGLIMGTMQRTRISSVKIMMI
                     LGANEGVLPANVSGEDILSEDEKMFLVNNGIEICKIDRLRNMEEDLAIYRNLTKAKDL
                     IWISWAASDTEGKRLKPSSLFSKIRDIFPNAEVKKDIVSRGDVFDLVESEKSTLNHLT
                     HALGRKKTGEALEEPWKEVMSWYREKGETELAMIKEGLLYKGKEKNPQKALTDILYGI
                     SEKGSISISPSRLECYGRCPFSHFVRYGLHPDERKQYEVGPADMGDIYHKCLMLLSKA
                     LTTDIPVTSEGSPWMTVNRDQCRQMVHDFVAAEKSNYREGILSGRGEQSYRSKRIEEV
                     CEEASWQMIEHVRRGRIEKMAFEEPFGRNKRIGPVEIWPGQEETEKETKIYIEGKIDR
                     VDMLPGQNVKVIDYKSGADKYDEKEVKAGIKLQLILYLRAATGKEKSEDKKRYPAGTF
                     YFHVKEPDIDAGDMDYSQNEIFRSELEENIRKAFRMDGVMVNKPEVLASISGGDTTVL
                     SSKGKGRAMEEKEFNDMLSAVYEKADGMCRSIVQGNISKSPKRKNAYTSCTYCDYRGI
                     CRFDTAFSQCRFEKI"
     gene            complement(39722..>40141)
                     /locus_tag="PLM92_00180"
     CDS             complement(39722..>40141)
                     /locus_tag="PLM92_00180"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HPR23593.1"
                     /translation="SFKERITIFPGMMEVLSEIKKRGYKTGLVTSRLAVTTAQGLSKY
                     DLWQYFDAVVTMEDCKKHKPDPEPLEITLKKLCSKPEKSIMLGDTMHDITCAKNAGVE
                     SVLVGWAIAVKKDEIKSGNGPDHIIEKADDLLEILEG"
BASE COUNT         9540 a         9906 c         7303 g        13392 t
ORIGIN      
        1 gcctactatt tctgcattcg gctcttttgt tgccctgctc tttacgaatt cgactcccat
       61 catggcgcct gtccctctca catcgcccac gacttcatat ttttctttcc attcattgaa
      121 tttggccatg catttttctg ctatctttct tgccttcgcc ggataatctt ctttttccat
      181 aacatttatc acggcaaggg aggatgcaca tgaaagggga ttaccgcagt atgttccgcc
      241 gatcgttccg ggtgttacgt catccattat ttctgtgctt gtaactactg cagaaagagg
      301 cataccgcct gctatggatt ttgctatcac cattatatcg ggagcaaatc ccgcttcttt
      361 gtagtagtct gatacaaaca ttctgccgga acgggcaaaa cctgtctgga cttcatcggt
      421 tatcatcatt attccatgct tgtcacatat ctgccttact gcttttaccc attccatggg
      481 tgcaggtatg aatccgcctt ctccctgaac gggctcaaac aggatagctg ccacactgtc
      541 agcaggagcc gcttctataa atgccatttc aagtttgtta acatagtatt ttattgcctc
      601 ttcatctgtc atggaatcat cgggtcttct gtacatgttg ggaaaatcca ctctgtatat
      661 accatcaggg aacgggccca ttcctattgc gtaggccttc ttaccggtca tcgtcatggt
      721 cagctgggtc cttccgtgga aagctcctga aaatactatg atgtttggtc ttccggtata
      781 gcttcttgcg atttttactg cgttttcatc ggcttcagct cccgaattta cgaacatggt
      841 ctttttcttg tcccctttta cgggtgctat tgaattgatt ttttctgcaa gagttatata
      901 gggttcatgt gtggttatat tcatcattgc gtggaaaaat ttttctgact gacccttcac
      961 tgcatctatc agttccggct ggctgtaacc tatgttaagt actcctactc ctccgatcca
     1021 gtccagcagt atattgccgt caacgtcttc tatcattgcg ccttcaccgc gttttatcac
     1081 cagcggatat gtgcatttga tagcatttgg cattgccgct tctcttcttt ttattatctc
     1141 ctgggccttt ggtcccggca gcggcatgtt aacttttggt agtgcttctc tcaacatttt
     1201 tgaatcctcc attttaaaat aaatttataa aagtctcttg tttatatcca gctctgcgag
     1261 cttgcgatac agtgttgctc ttgatagtcc cagttccttt gccacagttt cttttccgtc
     1321 accgcctgca ctgtgctttc tgaatttctt aaggattatc tctcgttcat attcttttat
     1381 ctgatctgcc agaggcagat cgctgtctga aacgctgata atgttttctt cggatctttg
     1441 aagtctcatg ggtacatcat ccattccgac gatctcgcca aaagccatat ttgtactgta
     1501 ttcaatggca ttttcaagtt ctcttacatt gcccggccag tcataggtca tataaagatc
     1561 tcttacttca tctgtgaatc ctctgagttt tcggttcata tatttattgt atttcttcag
     1621 gaaatgatcc atgaaaagtt ctacatcttc ttttctttcc cttaagggag gaatatctat
     1681 ggggatcaca cttaaccggt aaaagagatc ttctctgaaa gtgccttctt tgatcatttc
     1741 ttccagattt ctgtttgtgg ctgctattac cctcacattg accattatgg acttattgcc
     1801 gcccaccctt tcaaatctca tattctgcag cacatgaagg atctttacct gaagatgtga
     1861 cggcatatcg ccgatctcat caagaaaaat cgtccccccg tcagccatct cgaatttacc
     1921 tatcttacct ttttccgatg ctcctgtgaa cgctcctttt tcgtatccga aaagctcact
     1981 ttcaaggaga ttttcgggga tcgctccgca gtttactgtc acaaagggct gtcccgccct
     2041 gggtccggaa taatgtattg ccttggcaaa catttctttc cctgtgccgc tttcacctgt
     2101 gataagaact gttgaatctc ctcttgctgt tatcagtgcc tgattcttgg ctcttgttat
     2161 ggatccgctt ttgcctatta tatcatcaaa agtgtttttt acttttctta tgtttatgtg
     2221 atatgccagt ttttctgctt cctcaatgtc tctgaatgaa aggactactc cttctatctc
     2281 gttgccggaa aaatacggct ttgccgtaac tattgcatga agtccgcccc gctcacctct
     2341 gtaaagctct tcgttttctg tgtagccctt gccggttttg aaaacatcaa gggcaggtga
     2401 actgagcatg tacttgttta tatgttctcc tatgatatcg ttttttgtgg ttttgagcag
     2461 ttccgccgcc gtactgttgc agtgtctgat atagcctccc ttatcaatgg caaatatccc
     2521 ttcgtgggcc gtctcaagaa ccgttatcat ttctttcctt gaaagttccg tcatctgaag
     2581 gtcttcctgc tgtgctgcct tggctgccag gagatctgcc attttttcca caaaggtgac
     2641 catgcttctg tctttgtcca ataaaattcc ccgctgctct tcgttaaaag caacgaggcc
     2701 tattattcct atgatctcat catttctttt tattggagtg catatctcgg ccagttcgcc
     2761 aactgttttt gccctgtctt tttgttcatc ataaaaccgt tcgcttctgg tatcttcgac
     2821 atactgtgtg acaccgttgc gcaagaccct ggcataaaga aaatttccgt ccagctttcc
     2881 ggcttcctct ttctgaccta tcatatcaga ataaagccct gtacctccga taatagtaag
     2941 agatgaatca actatctcca cctctacccc aacagcaata ctgatggctt ctgccacttg
     3001 ctgaacactt attgaaatat cctgcaataa agacatatcc tgctccctat tccgtgacac
     3061 aattatatca catttgtctc attcttgata ttattttttt tgttttttta attatatttt
     3121 ttctttgtct caattttgtt catccctgtc ggtcaggaca gaaccaagac tcagagatgc
     3181 cttttccaac tgtaggacga tgttgtttat gtccaccaat ttctttgggg aaatctcact
     3241 tcccacggaa agacttgtat tataaaggga tttttctatg tactcatgga catctgcgat
     3301 cctttttctt atggtctcca tcgtttcttc atcaagggca tctgccagag cctcttcctt
     3361 gctcagcatt tctccgtgat ccagttcaaa ttcggatatg ccctcaacgg taacgatatc
     3421 ataacccagc ttttctttta ttgccgcagc aagatcggct ttggcatcag tctcgccatg
     3481 taccagaaat atctgttttg gttcctttat aaatccgctt atccattcca gaagtccttc
     3541 tctgtctgca tgagcagaaa atccttccag atcatatatt tctgcgttga catgtatttc
     3601 ttctccgaaa agtgtcacgt ctttcagccc gttaagaaga ttccttccca gtgttccttc
     3661 cgcctgatat cccacaaaga ctacactgga tttgggatcc cacagattat gtttgagatg
     3721 gtgtcttatc ctgcctgcct cacacatgcc actagcggaa atgattatct tgggttcccg
     3781 gttcatgtta agcgcccttg aatcgtcact ggttcttgtg aatttcaggt ttttgaagtc
     3841 cagaggatta tctcccttaa gtatatattc ctttgtttcc tcatcaaata cctgcgcatt
     3901 ctttttgaac acttctgtgg cagtggtggc catggggctg tctatatata catttatttt
     3961 atccagcttt tctttgtact gttcgttttc ctcataaaaa cgattgaact caaaaatcag
     4021 ttcctgggtc ctacccactg caaacgaagg tatgaccacc gttcctcccc tatctgtggt
     4081 ttttattaca atatcaagca actgatctat gctggcggag ttttcgggat gcagcctgtt
     4141 gccataggtg gcttccataa taagataatc cgcttttttt atgattttcg ggtttcttaa
     4201 tatgggcctg tccatcactc ccagatcccc ggaaaaaact atcttggaga ttttttcctt
     4261 gccgttttcc tgctgttcat tctgaccgtt ttcaccggtt ttttctttta cccaaatctc
     4321 cgttatggcg gaaccgagga tatgtccggc ttcattgaat acagcttcca tttcatcatt
     4381 tatcttaaac agctggtcgt atagcaccgg gaaagtcagt tttaatgcct cctcggaatc
     4441 cttgatagta tatagaggtt caaccaaaga ccttcctgct ctttcatttt tgcggttctg
     4501 ccactccgcc tccttttcgt gtatatatgc actgtccttc agcataacgg gaagaagatc
     4561 tgccgttgcg tcagtacaga atattttgcc cttaaaccct cgcttgacca gaagaggaag
     4621 tctgccgcaa tggtctatat gggcatggct taaaagaaca cattctattt ctttgggttc
     4681 aaaaggaaat tcatcgaaat tctgggcctc ctgctgtttc cccccctgga actgaccaca
     4741 gtccaacagt attttgtgcc ctcctgcaat aagaagatga caggagcctg taactccaag
     4801 cgctgctccg caaaatttaa tgtgcataaa tgatcctcct tatatctttt tataaatcaa
     4861 aaagtcccga agaaagatac ctctctccgg tatcaggcaa aataacgatt atgtttttat
     4921 ctttgttttc tttttttgaa gccagcacag atgcagcata taatgccgct cctgaggata
     4981 tgcctgcaag tatgccttca gttttcgcaa gtttctttgc agtatcaaat gcgtcttcat
     5041 cacttacctt catcacttcg ctgattacgg acctgtcaag tatatccggt ataaagcccg
     5101 ctcctatgcc ctgtatccca tgggggccgg aagtaccacc ggtcagtacg ttggattttt
     5161 ttggttctac tgcaacgatc tttactgcgg ggttcttgtt tttcagaaaa cggcctgctc
     5221 cggttatggt gccgccggta cccacacctg aaacaaatat atctactttt cctccggagt
     5281 ctttccatat ttcaggcccg gtagtttcga aatgggcttt tggatttgcc atatttgtaa
     5341 actgacccgg catgaatgct ccccgggtct cacttgtcag gttttctgct ttttctattg
     5401 cgcctctcat tccccgggtg ccttctgtaa gataaagttc tgctccgtat gcagaaagca
     5461 gatctcttct ctccctgctc atgctgtcgg gcatgacaag tatggtctta tagccctttg
     5521 ctcccgccac cagggccagc cctatgcccg tatttccgct ggtcggttca attataacac
     5581 tgcccttttt cagcagtcct tttttttccg catcctctat catataaaga gcggttctgt
     5641 cctttatgct tcctcccgga tttttccctt cgatctttgc atatatgttc cccgaagaga
     5701 ttgtgccgtc atcctttgaa aactcatatc tgcttaaata aacaagaggt gtattcccta
     5761 ttgtttttat tatattctta taaagcataa ttttacctct atcatcatcc cacgctttgt
     5821 attataacac gaaaacattt atgaatgtat cctttgattc ttatattttt ctctttgcat
     5881 attcatataa aaaatgaccg tccctctttg aagagacggt cattcaatac tgattaggat
     5941 cagttggcgg gttttttatc agtcaaagtg ctgctttgta gcaactttta cctttttcct
     6001 tcccttcttc catttgattt ctcctgttgt gatgcaatcc tgtaccggac aaacattcaa
     6061 acaaagctgg catcccacac agtcatcttc aaggagtacg ggtcttcttt tcttttcatc
     6121 ccagtcaatg gcctgatgtg ctgcatcata gcaggatatg taacatctgc cgcatccaac
     6181 acattttttg ttgtcgaatt tcgggagtac tttgaaatct ctgttcagtt cctctgccgg
     6241 aatcagattg ggaagaacgc atccgatgaa atcgtcaagt ttattgtaac ctttttcttc
     6301 cataaaatgc atcatgccgc ttatcatatc ttcaactatc ctgtatccat actgcattat
     6361 agcagtagtt acctgaacgt ttctgcatcc taccgacagg aattcagctg catctttcca
     6421 tgtctcgata ccacctatac ctgacatttc atgaccataa ccgaaatcct tcttgttggc
     6481 tttcagaagt ttgttcattt ccggatcctg cattacctgt gcacagaatc tcagcgcaat
     6541 gggtcttact gctgccccgg aataaccgga tatagaagat ttgccattaa ctaccggcat
     6601 accggtattg ttttcgaaat ccacattcgt tatggacttg atcgtattaa ttgcggatac
     6661 cgctgctgcc cctcccttta tggctgccct tgcgggttct tccatgtttt tgcagttagg
     6721 tgtcatcttt gctatgaagg ggatatttgt tgtttctgaa acagcttttg aatatgcttc
     6781 caccagttta acattggttc ccacatcaga acccatgtca tgggaagtca tctggggaca
     6841 tgagaagtta ccttcaatca ggtctgctcc tgcctggtcc gccattttag ccagatctct
     6901 ccattctttg tcgctgcttc ccattatgga tgcaacaaca actttgtccg gataatcctt
     6961 tttgagttta taaatatact caaagttctt ttctgtcggt ttgtctgata tctgttccat
     7021 gtttttgaaa ccgagccagg gaattccttc tttattcgtc tggtcaaacc tgggtgaaca
     7081 ttcgtctgca acgaatatac ctacagtttt gaagaatact ccgccccagc ctgtctcaaa
     7141 gcatttggct accatgtcat aacagccgcc aacgggtgat gatgaaagga agaacggatt
     7201 ttcgcaatca actcccagat attttattga taaatctttt ttaactgcca tcctacttca
     7261 ctcctttctg taagaattct attatcgagg cggctgcttc tttgccggaa gctacggctt
     7321 caacaactgt ctttccgcca tttacgatat ctccgccggc aaaatatttc ttgctgctgg
     7381 tcttgccctt tgcagtggct tttatcatgc ccttctctgt aagcttgacg ggagccagtt
     7441 ctgagaagtc ctcagcaccc tgtcctatgg caaatacaac tgtattggct tcaagcttca
     7501 attccgagct ttcggtcacc tttggtctgc ctttgaactt gacggcttcc accttgtttg
     7561 ttcccagaac actggaaggt gccatgttag ttgtgatgcc tattcccagg gaaagggcat
     7621 actggaattc cgtcatgttt ccgggagcct cttcgagagt tctcctgtaa actatgcgaa
     7681 catcttctgc tcccagaagt tttgcagtca ctgcacagtc tacagcaaca tctccgccgc
     7741 ctactactac gacttttttg ccaggtttga aagttttctt tgttcttgcc tgtttaagaa
     7801 aatccgctgc tgcgtataca cccttaaggt tagttccttt gataggagga acttttgctc
     7861 cccaaaggcc aacaccaacg aaaacagcat caaatgcttc cagctcggca ggctttactt
     7921 tcttgccgag aacgaatttc actcccagtc ctttgacctg agctatgtca tgatccacga
     7981 ctttctgagg cagtctcgtg ggaactattc cgtatgtcat gactccgccg gctttttctt
     8041 ctctgtcaaa aacagttact ttgtagcccg ctttagccag ctccgcggca catgccagtg
     8101 atgccggtcc ggcacctaca catgcaactt tctctttcat tttctttgcg ggagccttca
     8161 gcgtcttcat cttgaagatc ttttcctgtt cgatcgcgta tctctgaagt ttacctatct
     8221 gaatgggttt gtcaatacca catctgctgc aagcttcttc gcaaagtctg tcgtagggac
     8281 atatccttgc gcatgatccg ccaagaatgt tgttttctct gatggtctct gcggcacctt
     8341 ttgcatttct gaatcttatc gatctgataa atttcccggg gtcggtccct gcgggacaac
     8401 cctcactgca aggtgcatca tggcagagta aacatcttgc tgcttcttcc atagctgtcc
     8461 tgtgattgaa tccagcctgg gcttcagcag tgtacttagc tttttctact ttactcaatt
     8521 tctactcctc ctttgataca taactaatat ttgctgacgc gaaggcgccg aataaaatat
     8581 atattagttc ttttttgtcg ttcatttgaa tgacgatgtt ctctaattct aacatacttc
     8641 tgcataaatg caagttgaac agcacctctg ccggtaaata tccggtgtaa aaaatacatc
     8701 cggcgggaag acttcccgac ggatgtatta tatttttttt ggacctattc gtaaaactgg
     8761 tctgctttaa gtacggcgcc taaagctgct gttgctcctg ccgtgcagtc ttcatcagaa
     8821 acaaattcag gttcacaatg ggacagtccg tcttttgttc tcgtgaatat catagttgtc
     8881 ggcaacatat atgcgcagaa ctgtgcgtca tgtccggctc cggagagtat cttctgccat
     8941 ttcattccgc tttcttccat tgattcttct acaaagcgaa cgagattctt gtcgaaataa
     9001 accgtatctc ttacccactg ttcttcgatc tcacatgcgc acttgcacca ttcttccttt
     9061 gccatatcct ttagtatgcc cattaccttt ttgcgaagct ccggatccgg atgtctcaca
     9121 tccagggaga actctgcttc atcgggaata catgtatgta tacagggatg aacatatatc
     9181 tcgcctgtgg tgtatacgaa ttccggttcc tgtcctttgt ttagttcatc aaatcttttg
     9241 tgcatttcac aaaggaatgt ggaagcagca aaaagagcgt catgtctttt acccatgggg
     9301 aatgttcctg catgagccgt gaacccgtgg aatctgactc tgtagttgaa catgcccatt
     9361 accagctgta ctgcacccac ttcattacct gcttcttcca ggatgggtcc ctgttccaga
     9421 tgtgtttcaa acattgcgca gtatttgtcg gggcttattc tgttttcttt gggtcccttg
     9481 aattttgttg ctgccagtgc ttctccgaaa tttgcgaact cggattcatc catgggtgtg
     9541 gatttcatca cattctcata cttaaaattc acacgcagtt cttcgggaat ataatcattg
     9601 catattatac ccgaaaccat catagcggga ggataaagag agccttcttc attggtccat
     9661 atcattgtcg ttatcggatg cttgtgaggt attttttcag ccactatcgt ttccataact
     9721 tccattcctg tcattactcc cagaataccg tcatagttgc caccgttttt aacggagtcc
     9781 acatgggaac ccagaactat cctctttgca ttgggatccg atccttcaat ggttgcatac
     9841 atgcaggcca catcgtcaca ttcgattttt gcgccgatag cttccatcct tttaacgaac
     9901 tcttctctgg ccatgtgtgc ttcaggggaa agagaatatc tggttattcc gccatgaccc
     9961 gcgtctccga attttgaaaa ggttttgatt ttgtcgctca ttctgtttag atcacatttg
    10021 taagccattt tatgcctcct taaattaata ataccattat gcctttcggc cgttttaata
    10081 aaatatatat cgatatgcaa cgcaaaaaca acacatcaca caccgaggta caataatatt
    10141 atatacctgt cttttttttt tgcaacatgt aaagaaaatt tacatgcctg attgcaatat
    10201 taaaaaatgt attattcatt cagtactgtc agttttttta ttttttgggg tttcttcggt
    10261 ttgtctctca tatctctttt ggtgcttacg atcctgtcag cctcttccat cccctctgtt
    10321 attctgccga aggctgcgta ctgtccgtca aggtagggcg catcatccac cattatgaaa
    10381 aactgagatc ctgcagaatt gggattcgcc gctctggcca tggaaagcac tcctctttca
    10441 tgaagaatat cattttcaat tccgttgtct gagaattctc cttctattgt gtgcccaggt
    10501 ccccctgttc catctcccat gggacatccg ccctgtatca tgaagccggg aataaccctg
    10561 tgaaacaaaa gcccgtcata aaaccccttg ttggccaggg cttcaaaatt ggccactgtt
    10621 tttggcgctg tattttcgta aagttcgcct ttcatcatct cgccgttttc catttcgatt
    10681 ataacttttt tcatttcatt atctccttat cttaaaaact tcctgtttct ctgtgccgct
    10741 ttctataaat tcccacaact cgtctctcaa atattccgga taaagatttt ttgttctttc
    10801 catctcttct ctgcccatga agcgaacttc cctgatgacc tgctcatttt cttctctttc
    10861 gggatcattt ccttttttga ggctgccccc ctttatttcc cccatgaaga aattgacaaa
    10921 tctttgctct ttaccttctc ttacctgttc cacatgccaa ataagcatgc ctatttccac
    10981 atcaaaacct gtttcttcct tgatttctct tgcagcagcc tgcctggaat cttcattctc
    11041 ttcaatgccc cctcccggaa gcatccatat atctttccct tcatgatgtt gccgtaccat
    11101 caacactctt tttttctcat cgaggatcac tgtcctcgca ccacctatcc acataaaaat
    11161 gctcctgctg ttcccattac tattattata atgatgggat ttattttgaa tttacccgac
    11221 agtataagtg tgaccataaa tatcccaagg gggataagct ctacagaaaa tggggccggt
    11281 ctgtatattg atgtgccggc gataaaaacc acagccgctg aaataaggcc gatcactgta
    11341 ggtctcatac cctcgaatat tccctccact atcctgcttt tcctgtacct tcccataaat
    11401 ttcatcacaa gtagtactat tacaaatgaa ggaagtgcga tggaaaatgc agccagtata
    11461 cctccgaata tccctgcata ttcgatcccc gtgtaggtag cagcatttgg agccactgct
    11521 ccgggtactg cctgggaaac ggcaataagg tctgaaaact tctcgggaga cagcatgtgg
    11581 tatttctgta ccatttgaaa gattaggggc agcatcgcta tcccgcctcc gaaactgaaa
    11641 gctcctataa agaaaaaaac catgaacaga tgaatgaact tcattggtga ccacccgcct
    11701 ctgttttttt tctttttact gccatatata caatgcctgc cgttcctccc agtattatgg
    11761 gtataacagc cattatttca aatataccta tggcgataaa aaccagaagc gctactgtcc
    11821 agtgaaatgg ttttcttaat actttttttg ccatatcaaa aacagtaaca gttatcaaac
    11881 cgcaggcgga agcctttatt gctgtaaaag ctccgctgat ataagtactt tggcccagcg
    11941 ccccaagaag aagcactact gcagtaatgc atatgactga aggcataagc attccaatgg
    12001 ttgccacggc ggctcctgtt atgccgtttc ttttgttccc gatatatgtg gctgcattga
    12061 cggcaattgc tccgggaaga gcctggcaga cggccagaca atctatcatt tctgtttctg
    12121 tcagccattt tttttcttca acgatcaccc tttgtattat gggtatcatg gcatatccgc
    12181 cgcccactgt aaaaaaaccg gttttgaaaa aagcaagaaa gagaacaaac aattcctttg
    12241 ccttgtcttt ttttgtcatt attattcccc gtttatcatg ccgacggttc tcttgatagc
    12301 ttcatcaaat gttaactctt ctttttttga tccgcaacgg agtttgtatt ctgcttttcc
    12361 ttctgcagct cctcttccta cggttatctg cacaggtata cctatcagat ccgcatcctt
    12421 gaacttgact cccggtcttt catcccgatc atccagtagc acatctgctc cggccttctt
    12481 aagttcgctg taaagttttt cgccctcttt catctgagtt tcatccgagg tttttacaat
    12541 tattattatc acctgataag gagcaactga caaaggccaa agtatcccgt cgtcatcatg
    12601 atgctgttct actatggctg ccagagtcct tgtgactcct atgccgtagc atcccattac
    12661 tatgggtctg ctcttctggt tctcatcctt ataatatgct cccattgatt cgctgtattt
    12721 tgttcccagt ttgaatacct gccccacctc tatgcctctt gcgattttca ccggttctcc
    12781 gcatgaaggg catgggtcat tttcccgaat gagttttata tccgctacta tatctgcttc
    12841 gtagtctctt ccatagttta cattctttag atgagcatcc tttttgcatg caccggcaca
    12901 aaggtttttc tgtccgggca gctcactgtc aacgactata gtcaaatcat gaagcccgac
    12961 aggtcccgta aaccctccga cgcatcctgt ggcaggcccc attttgccct catcggcaaa
    13021 atctattgaa tgttcgggta taccaagggc attaacaagt ttgaccatat taagctctct
    13081 gtcacccctt atgaaggctg ccacatatcc gctttctttt tcatcattgt cgtaagtgac
    13141 aaaaagaagt gcctttattg tctgatcctt tttgagtccc aggtatgcgg ccacatcctc
    13201 gatcgtcttc atgtcaggag tgccgacttc ctccaaaggc ttcatttcct cgtcatcgtg
    13261 ttttgcatcc ggggtttctg ccctttccac agtagccgcc atatgacagt tttcacaata
    13321 tgctatctga ctttctccca catcggaaag agcagtaaat tcatgagagt tgctgccccc
    13381 cattgcaccc gtatccgcat cgaccgccct gtactttagt ccgcatctgt caaatatttt
    13441 ttcgtatgca gcatacatgt cttcataact tctgttcagt ccttcttcat ccctgtcaaa
    13501 tgaatatgca tccttcatta taaattccct gcttctcata agaccgaatc tgggacgggc
    13561 ttcatctctg tattttgtct gtatctgata aaggttttca ggtagctgtc tgtatgagga
    13621 aatgtcgttt ctgactatgt ccgtaaaaat ctcttcatgg gttggcccaa gacaaaattc
    13681 tcttccgttt ctgtccttta gtctccaaag ttcaggaccg tatgcaaacc atcttccgga
    13741 ttcctcccaa agctcagcag gctgtactgc ggacatgtgg atctcctgcc ctcctgcgcc
    13801 atccatttct tctcttatta tttcctctat ttttcttaca gatctccatc ccataggcat
    13861 aaagccatac acgccggaaa caagtttttt tataatacct gccctgagca tcagtatatg
    13921 actgggtatt tctgcttcac tgggtacttc acgcagtgtt ttaaagtgca tttttgaaag
    13981 tctcattttt tttgcttctc cttcttgtct tttttatctg gtgcttttct ttattatctg
    14041 atttctgatt tgtcgactaa tacttaataa ggagacaggc cgcctctgca gctatgcctt
    14101 ctccttttcc tattgtcccc attttttcgg tggtggttcc ctttatgttt acaaagtctt
    14161 cttcaattcc catggcttca gcaagatttt cttttatttc ccgggtgaat tttgcgatct
    14221 tcggctgctg agccatcact attatatccg cattgcaaat atgatatcct ttttctctta
    14281 ttatgcctgt cacatgcctg agcagtttta tacttgatat gtcacggtat ctgtcatcag
    14341 tgtccggaaa atgcatgccg atatctccca ttgaagctgc tcccagcagg gcatccataa
    14401 gagcgtgaac aagaacatcg gcatcagaat gccccaggag acctttttca aagggtatag
    14461 taatgcctcc caggacaagt ttcctgccgt attcaagacg atgcacatca aatccgatgc
    14521 ccgttctgat ttctcttatg tctttcattt tcttgccggt ttcccgaaaa tcattcttcc
    14581 tgcagaagtc tgcagcacac tggtaactgt cacctctatg gttttaccta tatgccttct
    14641 gccgtcttca actactatca ttgttccgtc gtcaagatag gcaacagcct gattgttttc
    14701 tttgccttct tttacaagga aaagactcat gtcttcaccc ggaaggacca caggtttcaa
    14761 ggtgttggca agttcattta tattcagtac ttccactcct ttgatagatg ccaccttgtt
    14821 gagattaaaa tcattagtaa caacttttcc gttcattatc tgggccagct tcagtaattt
    14881 cacatccact tccggtatct catcaaggga cttttctcct gtggtattgt agatctcaac
    14941 cccgtattca ctttgtatct tgttaagtac atccagcccc cggcgacctc tgttccgttt
    15001 cagggaatca gaggaatcag caatatgtct gagttcaacg agaacaaatt ccggtatcac
    15061 gatatttccc tcaatgaatc cggtcttcat tatgtccgag atcctcccgt ctattattac
    15121 gctggtgtca agtatcttgg gcgtggcatc ggactgtttc ttgcttttgc cggatgaagc
    15181 cgttgctttt ctggctgaca tcaatgctgc aagaccttct tttccttttt ttgtggccac
    15241 cacgacccca agataaccca aaagaagata agtcaggacc gtaagcacag tcggcaggta
    15301 atcaacttct atgcccgcat agatctggct tatcaaaaat gcgatcaaaa gtcctgtcac
    15361 aagtcctgtg atccctgcta taagctcatt agtggaatat gactggagat cgctttcaat
    15421 gttcctggcc attttctggc tctgtctttt cactgatggt gtaagcctga aaaataataa
    15481 tgcgaaaata attacaaata ctatggccgc ccctatttgc tgccactggg taaatccccg
    15541 acccggccct atgtcgctta tggttaaaag gtattttgta agcaaaaatg ctccataccc
    15601 tattatgcct cccaccaatg tgaaaacaag tctcatcagt tttttaagca ttggttcacc
    15661 tcctttctgt tttgctattt tggtatgcaa agaggtcctc ttgtcatttc ctaaaaatca
    15721 gatggaatcg atgacaagtt cttcagcctt ggcagtttcc atatctctgg caagtattat
    15781 ttcactgacc agtatctggc gtgcattggc caacattttt ttttcgccgg cggaaaggtt
    15841 tttttccctt tcgatacgaa gaaggttcct gactacttct gccacttcac aaatatcccc
    15901 ggtcttgagt ttctccatgt tttctctgta tcgtctgttc cagttgtctg acatctcggt
    15961 agaaggtgca ttgagcacct caaggactgt gtccacatct tttgatgaga tcacgtccct
    16021 aactccgata tctttgcttt tttccacggg tatcatgatc ttcatgtcac cgcagggcac
    16081 attaaggaca taatactttc ttttttcacc caggatgact ttttcttcga tctgttctat
    16141 tatcccggca ccgtgcattg gatatacgat tttgtctcca atactataca ttattttcct
    16201 ccaggtcata cctaactatt agtataattc aaaacacgat aatgtgtcaa gcattcaaaa
    16261 ttacatagtt tatcacagtg ccttttcagt gtcaacattt ttttgacaca aattctacac
    16321 ttatttttct tttaatatgt tacaataatt ctaaataaat ggaggtactg ttatgcccaa
    16381 attactggta gtggatgatg aacaaaaaat aagagaagtg ataagagagt attccgaatt
    16441 caacggatat gaaataaccg aggcagagga tggtatgaca gccgtcggac tttgcaaact
    16501 taatgattat gatcttataa taatggatat aatgatgccc aagctggatg gtttctctgc
    16561 ctgcaaagaa atcaaaaaaa tcaaggacat cccaataata atgctttccg caagaagcga
    16621 agaatacgac aaactctttg gcttcgaact gggcatagat gactatgtaa taaaaccctt
    16681 cagcccaaaa gagctcatgg cacgggtaaa tgcggttttg gccagacgaa gcaagccctc
    16741 aaaggcagct gccgaaatac tggaatttga tggacttgtc ataaacatac cgggccgtat
    16801 agttactgtt gacggcgaaa agatagaact gacacccaag gagtatgatc tcctgttcta
    16861 tcttgtgaac aataaaaata ttgctctttc cagggataaa ctgctttctg atatctgggg
    16921 gtatgatttt ttcggtgatg acagaaccat tgacactcac ataaaaaatc ttcgcaataa
    16981 tctggggccc tacagagatt acatagttac cctgagggga gtaggatata agtttgaagt
    17041 ttaaattcga ctataagagt atcaaggcaa gagtatgggt ctattttttg tgctttaccg
    17101 tcatcgttct tatgcttgtc tggttccttc agatactttt tatcaacaac tattatgaag
    17161 aaatgaaagt gcgggaaacc aagcgcattg catcaaatat aacctccgac tatacggatg
    17221 gaaacatcaa tgatatcgtg agcgaagtag aaaagctttc tgaaaatgat gatatgttca
    17281 tacgtatcga atccctgaag agcggtatag ttttctctcc gtccgtcgat gatcggggat
    17341 atgagaacga aatagaaaca gtcaagggca agcttatcac cagtggaaaa aataatatat
    17401 ctatcataat aagcagcccc tacaacagcc gcaatgttct tgtctatgcg ggatttttag
    17461 atccggcaga agaaaacata ctctatgttt tttctcctct ttatccggtg aaatccacag
    17521 tcagcatact tcaaaaccag cttgtatata taacgatcat agctttgatc ctggccacag
    17581 taattgcata tatactttcc ataagaataa caaaaccgat aagatctatc atcagatccg
    17641 caggcaagct ttcagctggc cagtacggta tcacattccg gacggaaaaa acatattctg
    17701 aaataaaaga tctggccaat tctcttaaca aagcttctta tgaactggaa aaatccataa
    17761 acctccaaaa agatctgatg gcaaatgtat cacacgatct aaggactcct ttgaccatga
    17821 taaaatccta tgcggagatg ataagagatc tttcgggcaa cgacccaaaa aaacgcgatc
    17881 agcatctgaa ggtaataatc gaagaagctg acagacttaa tgccctggtg ggcgatatgc
    17941 ttactctgtc agccatgcag tcaggaaaaa tatctttgag caagagcata ttcaatataa
    18001 aagaagcggc tgaaaacgtt cttcttcctt atcgccttct ggaagaaaaa gaagggtaca
    18061 gaataaaatt catatgccgt caaaatatat atgtcaacgg tgatctggaa agaatgaagc
    18121 aggtcatctc caacctgctg acaaatgccg taaagtattg cggtgccgac aaaaccataa
    18181 ctctttccat caaacgcacc ggcaaaaaaa tgcgctgcga agtaaaggat aacgggatgg
    18241 gtatcaagcc cgaagaactt ccccacatat gggaacgtta ctacaaggcc agcagcaacc
    18301 atgtaagagc caccaccgga tccggacttg gactttccat agtaaaagag atactttcgc
    18361 tacacagggc agacttcggt gccaacagca agcccggtca cggatcttcc ttctggtttg
    18421 aacttgatac ggtcaaaaaa cgctgattgc ttctgttatg ctggaaactc cctttagctc
    18481 aattttcaat ctctcttctg ccagttttga tgcatttctt ttgggcaata tgactttttt
    18541 gaagcccatt ctctcagctt cttttattat tttttctgcg ttttgcacag ccctcagatt
    18601 cccggtcagg cccacttcac ctattacaat ggttccttca ttgcaaggtt ttgctgtaca
    18661 tgcggaatat atggctaaag ccacagccag gtcggcagag gtcccttcgg gtcttaatcc
    18721 tccaactatg ttcacatata tatcctgatt cataagggga agtccggctt ttttctcaag
    18781 aacggcgatt atcatattca atcttgaaag atccacccct atggaagacc ttctggcgaa
    18841 tcccacactg acaggagcag caagggcctg aacttccaga agcaatggcc ttgtgccttc
    18901 atgcactgca gtcacaactg atccgtcaga cccctcttca gttccttcga gaaaggtctt
    18961 ggaaagattt tctatttcga ccagaccttc gcccgtcatt tcaaatgctc ctatttcact
    19021 ggtggtacca aatctgtttt tttgtgctct tattatcctt agctcccggt ttctttctcc
    19081 tgtgagatcc aaaacacagt ctacaaggtg ctcaattatt ttgggtcctg ccagttctcc
    19141 gctttttgtg acatgggcaa cgataaaaac aggtgtaccg gtgaccttgc ctatccgcat
    19201 aagttcattt gcacatgctc tcacctgtga cacgctaccg ggtgaggatt ccataaatga
    19261 tgtatacatg gtctgtatag aatctataac aacaaagaca ggtccaagct cttcaacaga
    19321 agcacatata ttttctatat ttgtttctcc cattataaaa agatctttgc atatgctgcc
    19381 gcagatcctg tctgctctca ttttgatctg ttcttctgac tcttctcctg aaacataaag
    19441 cactcttccg tatttttctg cgattttcag agctgcctgc attatgacgg ttgatttgcc
    19501 tatccccggt tcgccggaaa tcaaagtgag agaacctttc accattccgc ctcccaggac
    19561 tctgttcaat tctcctattc cggtatctat tctttccggt ccttccgaag atcttatatc
    19621 tttcaaagca gccggctttc ttttcccttt ttttgctttt cctattcctc ttttgtcgat
    19681 accgtcaaag gataagacct tttcctctgt catggtattc catgctccgc atatgcactg
    19741 ccccatccat tttggacttt catatccgca ctcctgacat acaaatacgg tcttttgttt
    19801 ttttgccata gtatcctccg tacaattaag ggcggcatct gccgcccgaa aattactttt
    19861 ttcttaaacc aaaggcaatt tcttgcctat tatatacact gtttttttct ttatgcccca
    19921 ttttctgcac tctttttctg aactgagata tacgtctatc ttcttccctt tgacacccgt
    19981 gtcacatgcc tggcaaagtc cgtaacccgg cacataaagc catgtgctca gaggtattac
    20041 ttttttgtcg actgcgatcc tgcctattct ggcctttttg ccattggcgc aggtgccttt
    20101 tcctgtatat gcatacgctt tgaccttcaa cttttttttg tatgccatac cgttattggc
    20161 atctatggat gccgagcttc tgcctgaaat cttttttttg ccggattttt tatatgcttt
    20221 gattttatat gcatatgttt tattggccgt tatgcttgaa ttgctgaagc tggtggtgcc
    20281 ggcttttgtt atggttttta tcttcttgta tttgcctccc tttgaggctc tgtaaacata
    20341 atacccgctg acaccggaaa ctttcttcca tgttatcttt atgtgttttc ttgatgttac
    20401 ggagtatttc acatttatag gggctgcagg tatggttttt gccgagcata tccgggatat
    20461 gccgcttttt atgatggtcg tgccatcttc tgcaagtaac aatgcattta cataatattt
    20521 ataggttttg tttattttaa ggcccgaatc tataaagcaa agtgtctctc ccctcaccga
    20581 tcttataatt tcatacttgc ctgttacgct gttatatctg tatatttcat acccctgcga
    20641 gttctccact gcctgccatg agatacttat ctggctgtga gacacagttg ctgcagacac
    20701 ttcaggcggc gccgctgtca cttcagccat ggctccctta ggagcaaggg ccattgccat
    20761 tgcaaatatc ataataaccg aaatcagttt ctttctcatt gaccgttctc ctgcttgctg
    20821 atacttctta ctctactgat tcagtataat caggaaatgt gaagaacgtg tgaataaaag
    20881 gctcgaaact gacatttttg tcacttgtgt catatattcg ttttttcctt tcctttatca
    20941 ggtatcaggg ttttttgctc tcacatggta tatctgtctg acagagcccg catccgaata
    21001 tgtaatcacc cctttcaatg tgttttggcc agtattttga cactgcttca tcttcatagg
    21061 cctcacatgt gtatttttca tggccctttc ctgatatggc ttctacagga catcttttta
    21121 tgcatttgcc gcaggagcca tcctgataat aaagacacca gtcatagggt cctctgtctc
    21181 ctctgggtgt tatcataaga tccgcttcta ttatcaggga ggatatcctg atagccttgc
    21241 ctttttctga aatgaatccg tctgaaagac tgaatgttcc caggcctgca gcaaaagcgc
    21301 tgtgcctgtg tgaccatttt gatgctatcc ccagattttc cgaatgcatt cttttgaatt
    21361 ccttctgaag atctacagat acacatctta aaccttcatc ttcgaggcgt ttaacaagtt
    21421 taccgctgaa ttcctgcatt agaggttccc attcccccct ggaaacaacc cagttgtccg
    21481 acgggaaaac agtggctttt ccctgatgct tttttgttgt ttttgtctga ggaaaaacca
    21541 tggatactac acgaagcttt tccgccttcg gtacattctt atatttgagc ctgaaaacct
    21601 cctggggatt ccagtgaaaa gatcctatat ggtctttaag aaaatcataa taggggtcgt
    21661 tgcctgctgc aacgccgata acaggtcttt cccacatggg gtcttccggt cttcccagtg
    21721 ttgcaaaatt attcttttcc aatgaatcaa aaatattata tactatttgt tttatttcca
    21781 ttctcatatc ctcacattgc cagtccggtt cttacttttt ccataatgtc ctgtcctttt
    21841 agggctttga ctatttccag tgcaaacagc attgcagttc cgggcgagcg tcctgtgatc
    21901 accttgccgt cttttaccac gggtttttcg gatggcctcg caccttttag ctttttttcc
    21961 agaccgggat atatgacagc ttccttctcc tccaaaacag aaagacctgc aaaaaccata
    22021 ggagcggcac atatggcaga caaccacttg tccttaaggg caaattctct tatcctgctg
    22081 caaagcccgt catgtttctt cagattcaaa gtgcctgtca tccctcccgg caaaactatc
    22141 atttcacatt tttcatagtc cgcatcggcg aaatccatat ccgcttttat ttttattcca
    22201 tgtccgccgc aaactgtcat atctttttgc cctcccggca ctgaaacaaa agccccttct
    22261 attccggctc tcttcaaaat atccattatg gtcacggctt ctatttcttc aaagccttct
    22321 gccagatgta taaataccat gtatgacacc tccttttatt ttatatggat tctctttgtt
    22381 caaaaaaacg gcccgtacac atgtgcaggc cgatgacttt ttttattttt cttcctcttt
    22441 tttgtgttcg gctattattt tctctgcaag atgcccgggc acaggttcgt atctttcgaa
    22501 gttcataatg aaatttcctc ttgcctgtgt cattgaacgc actgtgacag cgtaatcaaa
    22561 aagttccgcc tggggagctt ctgccattat cttctgacct cctcctgcca ggggttcaga
    22621 gccgaggatc tttcctcttc ttttgttcat atctcccatt atgtctccca tatactcatc
    22681 ggggactgtt atttcaatat gcattaccgg ctccagcagg cacggattcg catctactat
    22741 accttttttg aaggccagtg aagccgctat cttgaaggcc atttcgtttg agtctacatc
    22801 atggtaggaa ccatcataaa ggtctgcttt tattcccacc accttgcagc ctgccagagg
    22861 ccctttgtcc atggattctt tcagaccttt ctctacagcg ggaacataat tcttgggtat
    22921 ggatccacca aaaagcgatt cggtgaattc aagattatcc tgctgagacg gagaaaatct
    22981 tatgtgtaca tctccgtact gtcctgcccc gcctgtctgt tttttgtgtt ttccctgaac
    23041 atcggaactg cctttgatcg tttctctgta agcgattttc tgattgactc tgtcaacatc
    23101 cacgccgaat ttatccttga gttttgcagt tattacattt agctgctgtt ctccctgtcc
    23161 tcccagcaga gtctgttgag tttctgtgtt tctctgaaga atgaaagaag gatcttcttc
    23221 catcagtctg ttgagtcccg ttccgacttt ttcttcatcg ccctttgatt taggttctac
    23281 agctataaag aaagtgggct gcgggaagtc gatgggcgga aacactacta tactgttctt
    23341 atcacagaga gtgtctcccg tcctggtatt ctgaagtttg cctatggcta ttatatctcc
    23401 tgcttccgct tctgccgtat ccactcttgt ctttcctctt aagaaaaaca tggttcccag
    23461 tttttcggat ttttcggaaa ccgaattgta tatttcctgt ccgcctttga gtttgcctga
    23521 aattatcttt ccgaaagata ttttgcccag aacctgatcg gcaacggttt tgaatattag
    23581 tatggcaggt ttgccggaag gatcacattt cacttctatg tctttttccg agccgtcctt
    23641 tgccggatac ggtgcgtgat cagtggcttt tggcacaagg tctataaatg tctgcagagc
    23701 ctctttcacc ccggttccct catgtgcaga gcaaagcata acaggggaga tatccccttc
    23761 tcctatgcct ccggaaagac ctttgttgaa ttcttcttct gtaaattctt ctccgctgaa
    23821 aaatttctcc atcagggcct cgtctgtttc ggccaccgct tcgttaagag catctttatc
    23881 gtcaagggtc actacagatg tgccgaattt tgacttcaga tcggcaataa gctgatctgt
    23941 atcaacattt tctttgtcga ttttatttat aaggaagaat cttggcactc caagtttctt
    24001 gcatgaatcc catgcttttt ctgttcccac ttcaataccg gaagaagcat ccaccaatat
    24061 ggcggcagcc tcaacggctc tgagcgtcga gcttacttca cctgcaaaat caaaaaatcc
    24121 cggtgtgtct acaaaattca tcttgacttt gttgaattcc acaggcacta aagatgtgct
    24181 tatggaatag cctttttcta tttccatctt gtcccagtcg gagaccgtat ttccgtcctc
    24241 gacctttcca agtctgctta ttactcctgt tgccagcaat gccgcttcca gaaaattggt
    24301 ctttccggat ccgccgtgtc caactaaagc aacgtttctg atggtttcac tggtatagcc
    24361 tttcatatgc tacctcctaa atagtttttt caaatacagc gtgagtatta tatcatttgt
    24421 aggaatcatt cgcaataata attgaacaat tagacgcagg tttggtctct tttagttcct
    24481 tcagaccttt tcatgttcca tctttctgaa ataaggaagc cttgccgcta tgcaagttac
    24541 gaaaaaagcg ataagcagtc ttactaccac aacaacaaaa acatttgctc cgtttattcc
    24601 aaacagaaaa cccgtttcaa ttataccgtg gcacaaatac atgaaaaccc caattagagc
    24661 gaaatccttc ttggaaaggg gcttcctttc attgagttcc atcagggtgc cggctccata
    24721 ggtcactccc attacggtgc ccaccagcaa aggaaatatg gcatccctgc tcattcccat
    24781 gggttttccc ataaactcaa gacgctttga tattttttcc agaatattat actggacaag
    24841 caattctatt attatcatga ggggtatcaa tactttaagc agattcagaa cagttgaaag
    24901 gctccccgta agcatttcgt ttaaaatgta actccagctt tcactcacag aaacacacct
    24961 cccagccatc cgataaatat gcccaccaaa actgcggcaa ccactctgta aatcgccagt
    25021 ttgcctgagg aaaatccgat cttgtgagca atggcggttt ccacaggaat ggagtgggcc
    25081 ataaggttga ccatggcaat agtagtaatg gcggctgaag aaaattcaaa accgcccatc
    25141 gctgccacac agccgtattc atctgtaaaa attcctgtta ttatgggcac cgccgcatct
    25201 ccgggcaagt tgaaaagttt cattaccggc tgacagattt cctgaagcca cggcatcacc
    25261 ggcgagtatt tgagtaatac aacaagcaag tatatgggta acataatttt aaggagcata
    25321 agtccggtct taagcccctt aaaagttccg ttttttatag cattgaagaa tttcttcatc
    25381 ttatcctctt acagatatca tcctcttcat tttttaaaac tcggccgttc tgggagttct
    25441 ggggaaaggt aaaacatccc ttatgttgct tatacctgtc aggtacatta tcattctttc
    25501 aaatcccagc ccatagcctg aatgcttcac tcctccgtac tttcgaagct ccatatacca
    25561 ccagtaatcc ttttcgtcaa ggcctgcttc gtccatgcgt tctttgagtg tatcatatct
    25621 ttcttctctc tggctgcctc cgatgatctc tcctatctcc ggtacaagaa gatcacatgc
    25681 ggcgactgtt ttaccgtcat cattctgcct catgtaaaag gccttgatct ccttggggta
    25741 atctgttacg aacacgggct ttttatatat ttcttctgtt atatatctct cgtgttctgt
    25801 ctggagatct attccccatt ccacaggata ctgaaaatct ttgtgggatt ttttcaaaag
    25861 atccactgcc tccgtatatg tcactctggc aaaatccgat tctactatat tttccaggcg
    25921 ctttataagg cctttatcaa taaaaccgtt gaaaaactcc atttcttccg gagcattttc
    25981 cattacatac cggatgatat atttcaccat tgcttcagca acttccatat tgccctcaag
    26041 gtcgcaaaat gccatttccg gttctatcat ccaaaattcg gaagcatgcc ttgcggtatt
    26101 ggagttctca gctctgaatg tgggcccgaa ggtatataca ttgcgaaagg ccaaagcgaa
    26161 tatttccgct tccagctgcc cgctgacggt caaccctgcc ctttttccga aaaaatcttc
    26221 agaataatct atcctgccct tttcgtctct tggaggtttt tccatatcaa aagtcgttac
    26281 ctggaacatt tctccggctc cctcggcatc actgcctgtt attatgggag tgtgcacata
    26341 tacaaaatca ttttcctgaa aaaacctgtg tattgcgtaa gccgttatgg accttatgcg
    26401 gaatactgcg gaaaaagtgt tgcttctcgg tcttaagtga gctatctctc ttagatactc
    26461 aaggctgtgt ctcttttttt gcagcggata atccgcatcg gatccagcta aaacttcgat
    26521 ttcaaaagct tttatttcaa aaggctgctt tgaatcggga gtaagaacaa actctcccct
    26581 gaccttaagt gccgcagaaa tcggcacctt tgatatttca tcgaagttat tgataacttc
    26641 ggcttcatat accacttgca ccgatttgaa aaaggatccg tcattaagct cgatgaatcc
    26701 gaatttattg gaacctctgt tggtccttat ccatcctctt actgatatat cctgtccttc
    26761 atattctttg ggatctctgt aaagcttttt tatttgcgcc tctttcatca tttctcctgt
    26821 ggtttcatag tgggaaaaag tattatatct cttatgctct gtgcatctgt cacaagcatt
    26881 atcactctgt ctataccgat ccccagcccg cctgtgggag gaagtcctac ttccagtgcc
    26941 tccacaaaat cctgatccat tggatgtgct tcatcatctc ctgattcaag ctgttcctgc
    27001 tgatctttga atctctgata ctgatctatg ggatcgttta attcggaaaa agcatttgct
    27061 atttcccatc cgtttatata tgcttcaaac cttcttgtta tcctgggatc ctccggatct
    27121 ctttttgata atggagaaat ctccacggga tggcccgtga caaaaacagg tccgtcaaga
    27181 taaccgggga catcttcaca ctgctcttca aaaagctctg ctattacaag ccctcttgtc
    27241 atcttttccg caccctcaag cccgaatttt tttgcaactt ttctggcctc ttcatcggtg
    27301 tctatttttg aaaaatccac gccggttatt tcttttacgg catcttccat tttgagtact
    27361 ttccacggag gtgtaagatc gaaccgcttt ccctgatatg ttatcttcat ggaaccggtt
    27421 gctgccagag ctgcctttga caccacctgt tcggtaacct ccattacatc ttccatattg
    27481 ccgtaggcca tgtagcattc catagaagtg aattcaggat tgtgattctt atccatgcct
    27541 tcgtttctga acattttgcc catttcataa actctgtcaa gcccgccaac ggtaagtctc
    27601 ttcagataca gttcgtttga tatccgcagt ttcatttcaa ggtccagagt attatggtaa
    27661 gtttcaaagg gtcttgcgtt ggcaccgccg gctatggtgg tcaatatggg tgtgtcaact
    27721 tcaaggtaac cgaaatcctc ttcaagcact ctctttatct ctttgatgat tgaagccctt
    27781 tttatgaata gttcttttac ttccggatta acgataagat ccacatatct tttgcggtac
    27841 cttagatctg tgtctttaag accatgccat ttttcaggaa gcacctgcag ggattttgaa
    27901 agcagtacca ctctttgggc ttctatggtc acctctcccc gctgagttct gaagaccttt
    27961 ccttcaactc cgataatatc tcctatatca tatgtcttga accacttata ttcttcttcg
    28021 ccaagaacat ctcttttgat atgaagctgg atccttcctt tataatcctg aatatctgta
    28081 aatgaggcct ttcccatgac acgctttgcc attatccttc cggaaacaga cacatctttt
    28141 ccttccatac ggtcaaaatc atcttttatg tcctgactgt aggcattctg atcccatttc
    28201 tctattaaaa acgggttcct tccggcttcc tgaagggcct ttagtttttc tcttcttatt
    28261 actctctgtt catttagatc ggattcgttt tccctgtcaa ctgtatcctg tactttttta
    28321 ttatcattca ttttatcacc tgtttatgct tataacttca tacctgatcc tgccctttgg
    28381 ggcttctata tcaactttgt cccccacttt ggctccgata agaccttccc ccacctttga
    28441 ttcattggag attttgcctt caaaaggatc tgcttctgtg gcgcctacga ttataaagtc
    28501 ctggatcttc cgggtctttg tgtctttgac agttactttg ctgcctatgt tgactttgcc
    28561 ctttggagat ttgccttctt ctattatttt ggctttgcgc atcatgtttt ccagtttggt
    28621 gatacgttct tccagttccg cctgttcatt tttcgcagca tcatattccg cgttttctga
    28681 aatatcgccg aattctctgg cttcttttat cctttcggct acttctgcac gtttgacaga
    28741 tacaagttca tcatattcag ctgatatctt gtcatatcct tctttggtta aaagtacttc
    28801 ttcagtcatt tttagtccct catttccgac cttttgcagg tcatttcagc ccttggccgt
    28861 attgaaatat tataaaagaa aggtgcttct ttgtcaattt gcaaaggtta tctttacatt
    28921 gggagaattg gtcgcataaa cgattgttgc gtaacagaga tcgaactcca taatgtctcc
    28981 cacctttata tccctttcag catcctgtat atccgctatg gtatggtcgc tggacgcgcc
    29041 caggatctct attcctttat cccttggata gatcatgtcc ggaaaagcat agtccacttt
    29101 tcccagagca agcaaagctc ttcttcttat gcctctgtcc tcatactcct gtttcatgcc
    29161 gaaagcatca aacattatct ctccaacggg atgacttggc ttgtccttta cctctatcac
    29221 ttccgccttc agagtgaaaa catcctggta catgaaactc atgtcatacc cccaaagatc
    29281 cctcaggtct ttagcaagta ttattccctc tcccacacgc agcagattta tcctttcagg
    29341 catattcttt tcaaataccc tcgggagtga agtcgttgcc cctcctgaaa tgaagtcaag
    29401 tcttcttcca atgcggcttt ctatgctttc tgcgatattg accagctctt caagtttctc
    29461 gggtgttgcg cttactgagc cgtaacatcc aagattagtt cccacacctg aaagttcaag
    29521 accctcaaaa ctgttttcaa ccatcaaagc cacactgacc atttcctctg tgtcccaaaa
    29581 accctctctc aggtctccca gatccgccat caggattatc ctgtgttttt tgttctgcat
    29641 cagcgcctgt tcattgaagg ctttcaatac ttcgatttca ctgtgaagac ttatgtccgt
    29701 aagccttact gcctccgcag cctcggttat catgggcact cttatcagca tgaattcttt
    29761 tgttattcca tgcttctttg catcctccac ctgttccagt ctggatgtgg ctatgaagcg
    29821 gcattcagag tcagcaaact gtcttgtgca ttccggtata ccggtaaatc cttttactac
    29881 tccggctact tctatgttca gttccctgca acggactact atctcgtcaa tattatgcct
    29941 gagcttctta aggtctattt caactctcgg gtacatcatg cctcctgttt tctgttgctt
    30001 tccacaaatc tctttatttt tttggaagta tttttttcga actcttcttt tcttatgata
    30061 acctttttta tttttttgaa aaacggtgcg ttggcattta ttttgtcaac ttcttcccag
    30121 actttgtctt ccagctcttt atctgttatt cccttgccga aaagttcttc tacttcataa
    30181 tcgtccagtt taactgcggc aactatactt gtatctcttc cgtcactggt gtcttcgcca
    30241 aataccatgg attcctcaat gaagggaacg ttggaaagat agtattccag ttcttcagga
    30301 taaacatttt ttccgttctt tgttatgata acgttctttt ttctgcctgt tatgtacaaa
    30361 tacccttcat catccaggta gcccaggtcc ccggtataga accatccgtc ctttattact
    30421 tcggcggttg cttcgggcat ttcatagtac ccaagcataa cattccctcc ccttaaacat
    30481 atctcaccta tgccttcttc gttaatgtcc tcgatcttta tgccgaaggt ggggaatttt
    30541 ctccctacgg atgcagcttt gggtgctgtg tcgggattca aggcgcccat gggtgcacat
    30601 tccgtaagcc cgtatccctg aagtgcctgt ataccgaaat cctgtatgcc ttccagtacc
    30661 tcaggattta ttgcggcgcc tcccactatc atcattctca tcctgcctcc gaaaacagca
    30721 aggatgtcct tgaaaaatat cttgcccaga tctatgccga ttttctttgt ttttctgttt
    30781 atttttatta ctttcttcag ggttttttct ttgccttttt tgcgtacgtt ctgccatatc
    30841 tttttgtata agttctcaaa taatactggt actccaagga acatggtcgg tcgcacttcc
    30901 gaaagattct tgacaatctg tttcaacccc tcacaatatg cgatagccgc tcccttataa
    30961 agaggcatca aaaaaccgca tgtacattca tatgtatgat gtatcggcag gaccgaaaaa
    31021 aatatgtccc agtcattcac tttgagcacc gtgggagata ccatcagatc ctccacaaga
    31081 ttgccgtgag aaagcatgac tccctttgac aggcctgtgg ttccggaagt aaaaagcagt
    31141 acactcattt catccctgat tatctgagca tccaggaatt ctctgttgcc ttcttttaga
    31201 agttctcttc ctttttcaac aagacctttc caggatctga ctccgtcttt ttcttcatcg
    31261 gcgttcatat taaccaacat ctcaagagaa gatgttcctt ctcccttgat ctttgtgaat
    31321 atatctttgt acttttctgt gaaaaacacg cagcttactt cggatctttt taccagctgt
    31381 tccagctctt tggcattgag ttctttgtca aggggcacca ctatgcccgt accgcaaagt
    31441 actgccagat atgaaaccgc ccaccagtag cagttttcgc ctatgatggc gatcctgcga
    31501 tcttttaagc cctcgtttat aagggcagtc cccaaagcat ttatatcagc catggtttcc
    31561 ttatatgtta tttttttgta gtctccgcct tttttttctt tttgataaaa ggccacatga
    31621 tctccgtacc attcatcact ggcactggtt tctatcatat gctttatgtc cgttataggg
    31681 cggctgtccc tgtacttgac aagcgggtcg ggatctgcct tgattttttc ggctatttcc
    31741 actgcgcgct gcatgtccct ttcttttaga accttgtatt cttctttact cagcatttta
    31801 catcctcctg tccttcactg cttttattct cagcattgta acgtttgatt tttcttgtgg
    31861 tggttttact gaattcttcc tttcttatgc cgaatcttct gactctcttg taaaggggca
    31921 tttcctcatt tatatcgtct atgacacttt ttaacagttc cctcagtccg tcttcgttcg
    31981 tatctccctt ttcttctgct atgatctcat agtcgggaaa tatctctgcc tttattatgg
    32041 tatctcccgt cctctcatcc tctatcccgt gaacaagtac ttcctgtatg tacggacttt
    32101 ccgtcagaca aaattctact tcttcgggga aaacgttctt gccgtttttc gttacgataa
    32161 cattcttttt tcttcccgaa atataaaggt atccctcttc atccatatat ccgtaatccc
    32221 ccgtatggag ccatccgtct attatggtct ctttagtggc ttccggatcg ttataatagc
    32281 caagcatgac tgaggggccc tggcaaatta tttctcccat gccgttttca tcagggtcga
    32341 ttatctttac ctttgtgttt ggcataggct gccccacaga ggacgccttg ctgcaacagt
    32401 ctctgttaac ggcaattatc ggagaattct ctgtcattcc gtaaccctgt atcatcggta
    32461 tccccatggc ctcataatcc tcaattactc ttggatctat tgcggctcct cctgcaatga
    32521 actggaccat cccgcctccc gtctgctcat gcagctcttt gaatatcttt ttgataattt
    32581 tatcatgatt atataatttc agttttcttg atattattat catccttctg gccctgccgg
    32641 cagaaccgtt ggcctcaagt tttttccaga tttttttgtg catggtctca aatatcagcg
    32701 gtacactgag cataactgtg gctcttgact ccaccatgtt tttctggata tgttttaatc
    32761 cctcgcatat ggccagagtt atggcctgat aaagaccggt aaatatgtga catgtcaact
    32821 catatgtgtg atgcatgggc agaacggaaa gtccgactcc gtcttctatt atatccacat
    32881 actgggacat gttataaaca tttgatgcta tatttttgtg agaaagcata actcctttgg
    32941 ccaatcccgt agtgccggag gtgaaaagca aggcgcacat tacatccggg tctatttccg
    33001 catttgtaaa acgcctgtct cctgccgcca tcaacctgct tcccttttct atgagtttca
    33061 tcatcgaaag tctgtcatct gtatcctctt ccgctgtcat atcgatgaca tattccacat
    33121 ttctcacata tgccaggact tcatccactg cttttcccat tttgcgtgag tgaacgataa
    33181 cattggcacc tgatctctgt atcagatgag cgatctcttt ggggtgcaac tctctgtcga
    33241 tgggcaccac cactcctgtg ccgttggtga ctgccagata tgcaactacc cattcatagc
    33301 tgttttcacc gattatggct attttgctgt ccttaagccc catatcaaca aaacttgtcc
    33361 ccagagaatc tatgtcagca tcgaattttg aatatgaaat cggtttgtac tctcccccgg
    33421 gagtatcttt cacaagaaaa gccggatagg ctttcctttt ctctacagac ccttttatca
    33481 attctctcag atcccttatt tcccgtactt tataaaattt atctttatat ttcctactgt
    33541 tcatttcgat cctttctttt caaattatat agtaaagtat tatatcacat tgtggataaa
    33601 ttgaaaataa tatataatga agtcagtgta gttgttaacc aaaagagagg atgttttccc
    33661 cggaaagacc gtaaaaacta tgaaaaaatc aattgtgata tttattatag ctttgttttt
    33721 gggcatcctt tcgatcccct ctcttgccgt tgccaaaacc ccggatctca catactggtc
    33781 tgaacttgat ctgcctttcc ttgaagtcaa caaaaaactc cccgcagaat atgatcttcg
    33841 ggataaaaat ctggtcacct cctgcaagga tcagggatgg cacggcacat gctggtcctt
    33901 tgccgctatt tcttccgcag agaccaatct gctgatgaaa ggtcttgcag gaggagaaaa
    33961 aaaggatccc ggcaaggtcg atctttcgga gcttcagctg gcccgtttca tgtatgacag
    34021 aagccccgat ccctccggtg gtacaaaggg agacaagatc atcaattcca cagatgccga
    34081 ttatctggat gttggcggag aagcatatct caccactttt gctctggcat catggatggg
    34141 tcccgtaaaa gaaaaaaaag tggaaacttc ctatgaagac gccgataaaa gcaccaaact
    34201 tgacaaaacc ctggaatatg accttgacct ggctcatctt cgcaatgctt ggtgggtaaa
    34261 tgcaagagat cgggattccg taaaatccat gcttatgaaa catggctctc cgatcatctg
    34321 ctactattcg ggttactggg actacagtta ccatgaagtt ccgggcgcat cttccaccaa
    34381 tatgacttat tacaaccctt attccggaga aatgccggat catgaagttg ctgttatcgg
    34441 ttgggacgat gattatccgg cggaaaattt ttatccttcc cccggaaaag acggagcctg
    34501 gcttgtaaaa aactcatggg gcagcaaatg ggggaatgac ggatgcttct ggctctcata
    34561 ctacgatcat ggcattgatg attatgctgt gttcctggat ttcgacaaag cagacaatta
    34621 tcagtatata tatcagtatg acggaggagg ttcaactgcc caggatattg cttacaagaa
    34681 gaaagccttc atgtccaatg ttttcaaatc aaaaaaagac gaaaccctta aagcagtatc
    34741 tttctttgcc acacaaaatt ccaaaatcaa atattttatt cagatatata ctgatataac
    34801 agatccaaaa gacccccaaa gcggtcggcc caggctcagt gaaaagcaga caggcaggac
    34861 ctcctatgcg ggatactaca ctgttgatct ggattatccg gtgcgtctga aagaaaagtt
    34921 gcctttttca gtggtgatag agttcaaaac aggaagaaac gagaatattt ttcttcctgt
    34981 cgacactgac gctcaaagat acggctggtc agaatatgat gtcacctcag caaaaggtca
    35041 aagttttgtt tcaaaagacg gcgaggagtg gaaagatctc agcgctgacg gagataccaa
    35101 tattaggata aaggccttca ccgacgacag tgagcccctt tgggaactgt ggatagccgg
    35161 cgccatcgtt atcattgcct tttttacttt tcttgctgca cttatattca gggcctcaag
    35221 gaaaaaaaga gaagaaaggg cgaaaatata aaatgacctt ttttgtgtct gtcagatctt
    35281 ttgcttaaaa aaaccgtgct tattgcagta tgcatatatc atacctcttc cgctcctttg
    35341 tatcctgaaa ccgggatcct gttccggata aagcttcacc gtctgtatcc tgtcaaatgt
    35401 agcatataca aaaaaagaga tataatggtc tttttccata ggatgatcaa gagtcacata
    35461 gtactcatcc tctatttcat ttatactcat cctgtgctgc agcgaagcct catctccatc
    35521 ctcagctgtt tccgcttcca gaggaggcag cgttatcccg cagcagctga atgccccttc
    35581 tcctgtggag ctgatgacat tcccgcaaac cggacacaca tagaaattac atttgagcat
    35641 atttgctgat ttattgcggt tgatcatgta ttcccccgat aaaagctctg ccactgaaac
    35701 attaagagct tttgccaggg gctctatcag agttatatcc ggcaggcctt tgcctgtttc
    35761 ccacttggag atcgtcttgt cgctcacgga aagtatgtcc gccagctgtt tctgtgtgta
    35821 cttcttattt tcccgcagag ttcttattat ctccgataac atagttcgtc atatcttttt
    35881 tcctttcatt ttcctctttg gacatgatac tatttttcag gaacaaaaga aacctccgct
    35941 tcgtagagtt ttatatctgt aacccgtgac cgtttccaat catccgtatt ttgagatatg
    36001 tctttgggga tattattttg aaatatatct ccttgatata ttcctccatc cttacatatc
    36061 cattgctaat gctctttctg ctcatcaaaa aacccctgca aacttgaaaa ttcagagttt
    36121 acaaggggtt ttttcctggt ggagcatacg gggatcgaac ccgtgacctc ttgactgcca
    36181 gtcaaacgcg ctcccagctg cgctaatgcc ccctgctatg gagccacctg ccggggtcga
    36241 accggccacc tgcacgttac gaatgtgctg ctctgccgga tgagctaagg tggcattatt
    36301 ttcttccgtc aaaaacgtac aatggtattc tactatggca caaaacttcc gtcaagacag
    36361 acattaaaaa atttatccgg caatatattt attatagctt catattttct cgaaacggca
    36421 ctgggaaaat gcggtatcga atctgcatat ccctctgtaa tcgcaatatg tacatgatgt
    36481 gtatgcgttt tttctcttcg ggcttttgga tatattcccc tgcacaatac ttctgcacat
    36541 accgtctgcc ttctcatata ccgcagacag catatcatta aactcctttt cttccattgc
    36601 ccttcctttg cctttggaag aaagaaccgt cgtatctcct cctgatatac ttgccagaac
    36661 ttcgggttta tttaccatta caccgtccat tctaaaggct tttcttatat tctcttcaag
    36721 ttccgaacgg aatatttcat tttgagaata atccatatct ccggcatcta tatccggctc
    36781 ttttacatgg aaataaaatg tgcctgcagg gtatcttttt ttatcttcgc ttttttcttt
    36841 tccggtggca gctcttagat aaagtataag ttgaagcttt attcctgctt tcacttcttt
    36901 ttcatcatat ttgtctgccc ccgacttgta atcaattact tttacatttt gtcccggtag
    36961 catgtccacc cggtctattt tcccttcgat atatattttg gtctctttct ctgtttcttc
    37021 ctgcccgggc catatttcta cgggaccgat ccttttgttc cgcccaaatg gctcttcaaa
    37081 tgccattttt tctattcttc ctctccttac gtgctctatc atctgccagg aagcttcttc
    37141 gcatacctct tcgatccttt tgcttctata gctttgttct cccctcccgc tcagaatgcc
    37201 ttctctgtaa ttactttttt cggctgccac aaaatcatgc accatttgtc tgcattgatc
    37261 acggttgact gtcatccacg gcgacccctc tgatgttaca ggtatatctg tcgtgagtgc
    37321 tttggaaagc agcataagac atttatgata aatatctccc atatcagccg gccccacttc
    37381 atactgtttt ctctcatccg ggtgaagacc atacctgaca aaatgtgaaa aagggcatct
    37441 tccgtagcat tccagtcttg aaggacttat ggagatggaa cctttttctg atatcccata
    37501 aagaatatcc gtcaaagctt tttgaggatt tttttctttg cctttataaa gaagcccttc
    37561 ctttatcatc gccagttctg tttccccttt ttctctatac caggacatga cctctttcca
    37621 tggttcctcc agtgcttctc ccgtcttttt tcttccaaga gcatgggtga gatgattaag
    37681 tgtgcttttt tcagactcca ccaagtcaaa aacatcacct ctgctgacta tatccttttt
    37741 cacttccgca ttgggaaata tatctcttat ttttgaaaag agtgaagacg gcttcagcct
    37801 cttcccttcc gtatctgaag cagcccagct gatccatatc aggtctttgg cctttgtaag
    37861 gtttctgtaa attgccagat cctcttccat gttgcgcaac cgatctattt tgcatatctc
    37921 tattccgtta ttaacaagaa acattttttc gtcttcgctt agtatatcct ccccgcttac
    37981 attagcgggc aacaccccct cattggcccc cagtatcatc attattttta cgctacttat
    38041 tctggtcctt tgcatggtcc ccattataag cccatcggca gcaggaggaa ggactcctat
    38101 ctctaccgag gaaaatccgg cttcaaaaag caaggcataa tccttcatgg agatcttttc
    38161 ttgacctatt atttctacca tctgatccat tagtcccgct gtgacattcc atatctgtgc
    38221 tgtttcttca gcttcctcaa gatgtccgac atgcttttgc tgtgaaatca gtgtctctgt
    38281 tttttcaggc actcctgatt tatccgcaag aaaatcataa agtaatgcgg ttttttcatt
    38341 tacagtcctg gcttctttta gtccggtccc cagggctttc atgggctcca caagccgtga
    38401 tcttaaatca ttcagttctt ttatcccgtc ttttccgtat tctgtctccc cttttacaaa
    38461 ttcttttttc catgcggatc ctcttacttt atatttgtgt gaatagtttt ccagttcttc
    38521 gatttcattg ttttcaaaac ctgccagtcc tgttttcagc atactgaaaa catcctcggt
    38581 tttatatccg ccaccgataa tattaagcag ggaaataata taactgacta tggggctatg
    38641 cattatgtct cttttcctgt ccataaaaag ttctataccg tattctgcaa aaactctttt
    38701 gcagatactt ccccgctccg tcatgtcgtt acatatgact gaaatatcct tatagcttat
    38761 ccttttttct ctcacggttt ttcttacaaa agcagctgcg gtttctgcct ctgtataaaa
    38821 gtttgccgct tcgataagtt ctattccttc agttatatca tgtgcaccgg cgggcaatga
    38881 aaatacttct ttctccagtt tgtcaattac gggagctctc ttagcaagat attcccttcc
    38941 tatcctgaaa caattttcag gccggagcct ttttatcatt tcacctgtta taccaaaaag
    39001 ttctttgtcc ggaccacggt catcccaggt cataactata ttgacctcag cagatgcatt
    39061 acgtagtttg cttatgactt gcaaattttt tggggtaaag taatcaaacc cgtacaccca
    39121 aactgttttt tttcgtattt tttttgagtc ctgtattttc gccgaaaaaa tgtcgatctg
    39181 atcttctgtg tctatatact ttccttttat tcttttttca tattccgtat atattttttc
    39241 tatatccgac agttttctga aaagaaaact tttattatca acatgttctt ttatttctgc
    39301 cagcatttcc ggcgatactc cgtactgctt catttcggat ataaaattgt ttgtcatatc
    39361 tataaaagta ctgcttgaac aggatccctt gtataattcc aattcattgc ttatcttcat
    39421 tagtattctg gaaagcagca tatgcctgcc tcttttgtct atcagtgtct ttctcccgcc
    39481 gcctgtttca gatagtataa tggtggccag gcgggaaaag ctcattatct ccatatccaa
    39541 aagtatcttt tcatttaaat aaaaaaaggc atccctctct gcttgcagag tatactgatc
    39601 cggtactatc attattactt ctctgttatt ctgctgccgg cttcttttta tgctttcaaa
    39661 tatgaatttt tcttttatga gatcttctcg accataataa acattcagca tttgtctcct
    39721 ttcatccttc cagaatttct aacagatcat ctgctttttc tataatatga tccggcccat
    39781 tcccgctttt tatttcatct ttctttactg cgattgccca gcccacaaga acagattcca
    39841 ctcctgcgtt tttcgcacaa gtgatatcat gcatagtatc accaagcatt atgctttttt
    39901 cgggcttgga gcaaagcttt tttagtgtta tttcaagagg ctccggatcg ggtttgtgtt
    39961 ttttacagtc ctccatggtg actactgcat caaaatactg ccacagatcg tatttgctca
    40021 aaccctgtgc tgtggtcact gccaggcggg atgtcaccaa tcctgtttta taaccccgct
    40081 tcttgatttc cgaaagaact tccatcatac cgggaaatat ggttatcctt tctttgaaac
    40141 t
//
LOCUS       DAONHK010000002        22010 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_1902_length_22010_cov_4.997905, whole genome shotgun sequence.
ACCESSION   DAONHK010000002 DAONHK010000000
VERSION     DAONHK010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 22010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22010
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1902_length_22010_cov_4.997905"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            192..1799
                     /gene="tig"
                     /locus_tag="PLM92_00185"
     CDS             192..1799
                     /gene="tig"
                     /locus_tag="PLM92_00185"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HPR23594.1"
                     /translation="MKKFGWILFLTSFAASAVLSIILVAGTFGASGAVLSALLFPVTA
                     YVYPIYAAATIGGYYFIFPVVFYVLQIVAFLICSYGYGSEKLPGGIETEDEYEARGFG
                     KAAGGILKTGGMVLIFVTLSVLLGAFTRAAIIDGGVTWLLPVAAIAAAALVICIPMRM
                     KNGSRYNIRNRKKRDLIIAVVLAAVFAVSAYFGYDYFFGPAYSSYDLSKYIKVGQYKG
                     LEKEQTSIKVKDSEVKEKIDENLKAAATTEETEKGKVEKDDTVNIDYVGKINGKKFDG
                     GSAEGYSLTIGSGTFIDGFEDGLIGKEIGDPVSLDLTFPEDYSDESVAGRDVVFKVTI
                     NSKEVTVVPGLNEEFVQENSDSDTVKEYKKEIKDKLYKQKEKEVAEEQKTKLWNQVVE
                     SSEVLKYPKREIESLQNDLINEYKDYAKQYEMEFKDFLKQYAGIESIKEFKKQAKEYA
                     RIIIKEEMVLYYIADKEGITLSKEEYDAFVTKTLKTYGYTREQFEEAQGQSYEDAVGK
                     SVIERQIYMEKVKDFIFSKAKITGEEQ"
     regulatory      1960..2052
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            2126..3559
                     /locus_tag="PLM92_00190"
     CDS             2126..3559
                     /locus_tag="PLM92_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HPR23595.1"
                     /translation="MEQFTNILVSIDDVVWGVPLIILIMGTGILLTVRLGVLQFRHLG
                     KALKYMIHNEEGGKGEVTSFGALCTAMAATVGTGNIVGVATAIIAGGPGALFWMILAA
                     CFGMATKYSEGLLAVKYRTLDENGHALGGPFYYIEKGMGSRWKWLAKTFAFFGTAAGL
                     LGIGTITQVNGIASATKTFFDPENKNIAFSIGNMEYSWVTVITAVVVTVLAALVIIGG
                     IKRIARVATVIVPFMIMAYVTVCVSILIVNVAEIPEAVGTVFASAFGVRAAAGGALGA
                     MMIAMQKGIARGIFSNESGLGSAPIAAAAAKTNEPVRQGLVSMTGTFMDTIIVCSMTG
                     LTIMVTGAWERGLEGVDVTINAFETGLPWNSGTGAFILMLSLAFFAFTTILGWDYYSE
                     RCLEYLTGKNLKALSVFRWLYILAVLIGPFLTLSFVWTFADIFNGLMAIPNLIALVVL
                     NGVVVAESRSYFKRLNTGQLDDGFRKK"
     gene            3712..4188
                     /gene="nrdR"
                     /locus_tag="PLM92_00195"
     CDS             3712..4188
                     /gene="nrdR"
                     /locus_tag="PLM92_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013725719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HPR23596.1"
                     /translation="MRCPFCENEDTKVIDSRHTEEGHAIRRRRECEKCGKRFTTYEKI
                     EEIILMVIKKDGSREAFDRSKIMNGIIKACEKRPVPLSEIEKVVDDIERGLNNMMEKE
                     VKSTFIGELIMDNLKELDEVAYVRFASVYRQFTDINTFVAEIENLLGSKKKKGTQN"
     gene            4185..4835
                     /gene="cmk"
                     /locus_tag="PLM92_00200"
     CDS             4185..4835
                     /gene="cmk"
                     /locus_tag="PLM92_00200"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002600374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HPR23597.1"
                     /translation="MKDIFRVAIDGPGGAGKSTVAKEVANALGIDYIDTGAMYRAVGY
                     KIIREGIDPDDEETVEKMLSKTDIDFVKGDVMLDGEKVNGRIRTPEVSEMASLCSAYS
                     FVRKKLVAAQRKMGETKSIIMDGRDIGTNVLADAEYKFFVTASPDERAKRRQKELSEK
                     GEDISYEEVLKDIIKRDHKDTTRKLNPLKQAEDAILIDTTGMTVEEVVSKILKEIK"
     gene            4835..6076
                     /locus_tag="PLM92_00205"
     CDS             4835..6076
                     /locus_tag="PLM92_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004443389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HPR23598.1"
                     /translation="MALVRPFRAIRPNRQFAEKVVSLPYDVMNREEAEAMVKDNPYSF
                     LHISRSEIDLPEQKDPYAPDVYEKAKENLQQFLDKKIFIEEAKPMFYIYRQQMRDKIQ
                     TGLVGCVSVDDYFNDIIKKHEHTREEKELDRTRHFDVCSMHTEPVFLAYRDNKKIDTL
                     MEGYITNNPAEYDVTTSDAVEHKLWAITDDNVIAGLAGIFAEIPALYIADGHHRSASA
                     CQVGKKRREEKPDYTGEEEFNYFMAVIFPDKDLNIQDYNRVIRDLNGNTSEEMINKIK
                     EAGFDVEEKGTKQYFPEKKHIFSMFLEGRWYKLTAESSVIPRHITDSLDVSVLQDRIL
                     SPILGIEDPRKDKRIDFVGGIRGLDELERRVNDDMKIAFALYPVMIEDIMKVSDNDMV
                     MPPKSTWFEPKLGSGLFLHRI"
     gene            6171..6899
                     /gene="radC"
                     /locus_tag="PLM92_00210"
     CDS             6171..6899
                     /gene="radC"
                     /locus_tag="PLM92_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HPR23599.1"
                     /translation="MPDKTLMVHKYRKGNGIMILRELPENERPREKLIYRGKGSLSNA
                     ELIAILIRTGSRDMSALQLAEELLSMDKNGIFSLGECSVEELCAVKGIGKAKACQISA
                     AMELGRRVAATPRKSRQRIKGSRDIAGIYMEEMRYCKKEYFNAVMLNAKKEIIASENI
                     AIGDLCSSIVHPREAFLCAVKRSAAGVIFMHNHPSGDPRPSKEDIDVTERLCETGRIL
                     GISVLDHIIIGDGTYISLKEQGLI"
     gene            6936..7973
                     /locus_tag="PLM92_00215"
     CDS             6936..7973
                     /locus_tag="PLM92_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006001856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HPR23600.1"
                     /translation="MISFSSRDMGIDLGTANTIIYMKEKGIILNEPSVIAYDKNIGEI
                     IATGQKAKDMYGKTPANIIVVKPLQDGVISDFDMTSEMLQRFIKRSLGERKPSGLRVV
                     VGVPSGVTEVEKRAVEEVVRQMGAREVYILDEPTAAAIGAGLNIDTSEASMIADIGGG
                     TTDIAILAMGGIVVSTSLRYAGDKFNEAVIQYIRKKKGVLIGDKTAEELKINVGSALV
                     DKDTSGEENLYSMKARGRDLISGLPKTFEVSNKDMEAALYESMELIIDALKSTVEKAP
                     PEIAADIAEKGMVLSGGGGMIENLDKLISQRTGMIVEKAKDPQEAVALGTGKSLDNIE
                     KLRKYVDAKKR"
     gene            7985..8803
                     /gene="mreC"
                     /locus_tag="PLM92_00220"
     CDS             7985..8803
                     /gene="mreC"
                     /locus_tag="PLM92_00220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HPR23601.1"
                     /translation="MKWIRQHKIFSVTAVIVIALCLFVLGSYLAGGGSGVLGRGVQSV
                     IAAIEKPLSAFTGGARDTFKGIFRFSSVLEENENLKEENAILRQENIDLSMKKDELDR
                     LEELSKAFDFEPYSGQGKAVAARIISLNNSNTYDVFTIDAGSSKGIEKNDIVVDGEGL
                     IGKVKETGDSWAKVVSVLNENNSISFKVQRKTSITGIVAGDGKGKIAGYLMDNKATIV
                     KGDVLVTSGIGIYPEGIRIGKVTSVDYNDDMQLKEITVKSTVKFTSLQKVAVFK"
     gene            8800..9300
                     /locus_tag="PLM92_00225"
     CDS             8800..9300
                     /locus_tag="PLM92_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPR23602.1"
                     /translation="MSYKKAVLLFAFAFIMQLSIINLININGIVPDLTLCLVIMITFL
                     FEDGFRYGAIGIISALLLDLCTGQYVGISSLGMIIVMAGIVLLCRSMNRENKLSFVIV
                     TAAGTLIYGLLLWLFMAMLGDPTGILYVLMYQPFYMAYNIVFSFILYLLMIKKAVRHR
                     NDRYFV"
     gene            9297..12053
                     /locus_tag="PLM92_00230"
     CDS             9297..12053
                     /locus_tag="PLM92_00230"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HPR23603.1"
                     /translation="MNDFLSRYNKILFLLLALAIILLVRLFILTVIQNNDWSEAAEGI
                     STRNISTTAPRGEIYDRNGNLLAGNKQTFTVKMSAEDMTDKELNSVITKLIGILEDNG
                     DSFEDNLPIKAKNGNYYYTYETEIKKWLEKKGFPQDYTAEEAFFALRDQLGIDKQLDR
                     YDVQLEMQNMYNEYPPISVTRMKYNPEIEKEEFLESYGLSKNVNAEKAFKAIRKEMDI
                     SPGTSEKEARKIMAVRNELEMLGYKKYMPATISKNVSDDTVMIIEENSSELQGVETAS
                     ETTRHYPAGKSASHILGYMGKISEDKEEEYEEKGYEKGAVIGLSGIEGKYESILKGIN
                     GARKVQVNAYGDMVKEISDSEPEKGKDIYLTLDLDLQKSAERSLEQGLAAIRAGGGFK
                     SKYGSAPISKAAPAQVGAVVALEVETGDVLAMASYPDYDPNLFAEGISEKDWDSLQSE
                     NPRDPLAPSPLYNVATQSAVQPGSTFKMVTATAGLECGLNPYRLLRDGGHIEIGNKTF
                     ACVVWNMSKSTHGYVDMADALEASCNYYFYDVATGKDWADGGSLGYKKKITVDTITDY
                     ASQYGLGEDTGIEIPEAVIPVPSEERKTDNLKTSLKNVLYAAAETYFKPKIVKNKELL
                     EESIEKIISWVGRDIGRAEMINERLPKLGVRKSQIEPLADLCLYTYYNQADWSVGDAM
                     NIAIGQGENAYTPLQMTNYVATLGNAGKKHKVSVVKSIEDEGQTEEEQTEKVRVSSDK
                     YFDQIIDGMKQAAVGSRGSLGALFRNFPVEVAAKTGTAERGGYVPPKSEVTYIKQHLY
                     AFDSSLSWSKIKKEMNRLMKEYPETYTNENTAVRRAVINLSKRKITTETLDQFKSTYD
                     NFAWVVAMAPADDPKIAVCVMVVQGGTAANAGPIAREVIGDYLNVNGESIEYKTYNNE
                     TGIQ"
     gene            12068..12787
                     /gene="minD"
                     /locus_tag="PLM92_00235"
     CDS             12068..12787
                     /gene="minD"
                     /locus_tag="PLM92_00235"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HPR23604.1"
                     /translation="MSRSVLIASGKGGTGKTFFAANAGALLAMKGHKTVILDMDMGLR
                     DLDLYMGLESKVVYNIMDVLSGTCKIKQALIKDKRFRDLYIMAAATANDRRDVTPLHV
                     KILCEKLKERFDHIIIDAPAGIGEGLELAAAGADMAVVITEPEIASVRDADALDRKIK
                     DFGIAETYCVVNKVNGELMTSGVVPGISTISKGLRMEIAGMIQRDNNINISTNKGEPI
                     VMKKGTYIEKNMAKIIERILD"
     gene            complement(12824..13939)
                     /gene="rodA"
                     /locus_tag="PLM92_00240"
     CDS             complement(12824..13939)
                     /gene="rodA"
                     /locus_tag="PLM92_00240"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HPR23605.1"
                     /translation="MKKLDNFHDLFKNVSKPLILLPVFFCILSILMMFSTSYDNHIVF
                     SRDVIVQSAAYLIGFVLIIIIASMDYSVFEEIERPLYIGSVVFLLLPYLPFIGLEVNG
                     ARSWINLGITTLQPSEIIKITFVLLMANYLNRNRDKLYTLKSILMAALYAAPFILIVL
                     KEDFGSAVVFIIMWIVMIFFAGIDGRLFAKCAGITLLFIPAAYSLLDDYQQERITAFI
                     HPDNLALQGNYQVWQSKVAIGSGGFFGKGLFQGTQKDLDFLPVRNSDFIFSVVVEELG
                     FIGGAVLIAAYTWFIYAISKVALSAKDLYGTLVVMGFLGMFVFQIFENIAMCMGVMPC
                     TGITLPFISYGGTSILSCMVATGFIINVAIRNRGVTF"
     gene            14100..15644
                     /gene="rny"
                     /locus_tag="PLM92_00245"
     CDS             14100..15644
                     /gene="rny"
                     /locus_tag="PLM92_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HPR23606.1"
                     /translation="MPDIVLAVIVGLVALVIGILAGYIIRKTLGEKAIGSAEQKARNL
                     ILDAENKSETIKKEIALEAKEEAHRLRSEVEREIKERRNEISKSEKRLIQKEETIDKK
                     LENIERKEDSITSREQNIINKQKELETQIKKQNEELEKISGYTAEEAKAILLSNLEKE
                     IRVEASVLVKDIETKAREEGEKKAKEIITGAIQRCAADHVAESTVSVVPLPNDEMKGR
                     IIGREGRNIRAIETLTGIELIIDDTPEAVILSGFDPVRREIARLSLEKLIIDGRIHPA
                     RIEEMVNKSEKEVNASIKEAGEQSTFDVGVHNLHPELIKYLGRLKYRTSYGQNVLKHS
                     EEVAYLAGLMAGELGFDVKLAKRAGLLHDIGKAVDHEKEGTHVELGIELLKKYKESQA
                     IIDAMASHHGDYEPTTMEAVLIAAADALSAARPGARRETLETYIKRLQALEEIANNMD
                     GVDKSYAIQAGREIRIIAKPDRVKDEEMPFLAREISKKIEEELDYPGQIKVNLVRETR
                     AVDYAK"
     gene            15736..16929
                     /locus_tag="PLM92_00250"
     CDS             15736..16929
                     /locus_tag="PLM92_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012268760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HPR23607.1"
                     /translation="MIVYLDNSSTTKQYDEVTEIMFKCMQEEYGNPSSLHRMGFASEK
                     IIKKAREQVSEALSRSGLCAAAEGELFFNSGGTESDNTAVIGAAKARRRYGNKLITSE
                     AEHPAVLESFKRLKEEGFEISLIRVDENCRIDMEQLKSEINEKTILISLMKVNNETGT
                     IQPILEAAEQKKNALFHTDAVQAFCRVPQSVQNADMISISSHKIHGPKGAGALWIKKG
                     TNIKPLILGGGQENKFRSGTENVPAIAGFGIAAGKASDGMEEKMKRMSDIRKYLLEGI
                     KLEIADISINSPVYSCYEKKQETGEALCSPSILNISFLGTRGEVILHRLEQEGIFVST
                     GSACSSNKKGKSHVLSAMGLSEKESESAIRFSLSETNTIEEMDHVVYNLKKAVDKFRK
                     LGSLR"
     gene            16926..18110
                     /gene="thiI"
                     /locus_tag="PLM92_00255"
     CDS             16926..18110
                     /gene="thiI"
                     /locus_tag="PLM92_00255"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009644776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA U4 sulfurtransferase
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HPR23608.1"
                     /translation="MNEKNIYIIRCGETALKGQNKPYFERMLADRIKKILKKFPGIEV
                     KRHEGLIFVRTEKSTAEKDILKETGKVFGIASISPAVEVESDMEKIGTAAVSYMNDLI
                     MKKGIRTFKVEAKRADKSFPVKSPDIAKKIGAAVLRGCAVLSVDVHDPDVHLFVDLRH
                     DKTYIYQQKIKGFGGLPLGTNGRGMILLSGGIDSPVAAWMMAKRGMQIEAVHFHSYPY
                     TGQRAQEKVAELASILAIYCSRFKLHMINLLPIQEQIVQNCPEEETTVLLRRFMMRVA
                     EKVATDAGCGMLITGESLGQVASQTADAIIVTDEAVEMPVMRPLIAMDKVDIMDIAKE
                     IETYEVSIMPYEDCCTVFLPKHPVTRPKIERIKRSEERLCVDELINEALGSEEIRDIY
                     PK"
     gene            18572..19717
                     /locus_tag="PLM92_00260"
     CDS             18572..19717
                     /locus_tag="PLM92_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005838458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HPR23609.1"
                     /translation="MNFQLTKEQEFVRKMVREFATNEVEPIAADIDAEHRFPEENVKK
                     MADYGLLGIPFPKEVGGAGGDQLSYAIAVEELSRVCGSTGVICSAHTSLCCWPIYAWG
                     NEEQKAKYLPDVCSGRKLGAFGLTEPNAGTDAGGQQTRAELIGDQWVLNGAKVFITNG
                     GYADVFIIMAMTDKKKKTRGGISAFIVEKDDPGFSIGKTEDKMGICASSTTELIFQNC
                     TIPKDRLLGQVGDGFKVAMSTLDGGRIGIASQALGIAQGAFDVTVEYMKARKQFGKKL
                     SQMEALQFEVANMATQIEAARLLIYQAADMKEKNLPYGKYSAMAKLFAAETAMYVTTK
                     AVQLHGGYGYTKDYPVERMMRDAKITEIYEGTSEVQKMVIAASVFGK"
     gene            19737..20537
                     /gene="etfB"
                     /locus_tag="PLM92_00265"
     CDS             19737..20537
                     /gene="etfB"
                     /locus_tag="PLM92_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005838459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta"
                     /protein_id="HPR23610.1"
                     /translation="MAFKIIVCAKQVPDTNVIKINPKTGTLIREGVPSILNHDDANAL
                     EEALKIKDKYPDTHITVVSMGPPQAKDMLLECLAMGADEGVLVSDRAVGGSDTWATSN
                     AIAAAIKKIGEFDLIFAGRQAIDGDTAQVGPQIAEKMDLPQVTYVQEFEIEKDMKSIT
                     VKRALEDGYEVIKIKTPCMLTAIKELNAPRYMTIGGIFGANEKPIHVWNAKDIAIDLD
                     TVGLEASPTNVFRSFTPAPKGKGVILEGDTEKEVAEKLLIGLKGKHVV"
     gene            20556..21872
                     /locus_tag="PLM92_00270"
     CDS             20556..21872
                     /locus_tag="PLM92_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012448315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HPR23611.1"
                     /translation="MAIEIIKDKCIGCGICFKSCPMDAFEFEPYDGNKLGKVAKVNSK
                     CTFCNQCLTACKYGAIIEKKTQGGEVDFSAYKHIWVFAEQRNGKLMNVALELIGEGYR
                     LAKEISPDTKVCAVIIGDKDAKVDALEKECYEYGAESVIMIQDPLLKNYTTDGYTKVL
                     TDAIQEMKPEIVIYGATHIGRDLAPRVAARLDTGLTADCTRLDVKVSSYIEYAKKNTT
                     LDTSTLDPNDESTGIKQTRPAFGGNLMATIICPRTRPQMSTVRPGVMQKRERVSGAKG
                     ERVDYKPVISKDDIRIEIIDIVKSAKELVSLTDAEIICSGGRGLGDASGFELIQKFAD
                     KVGGVVGSSRAAVDSGWIDHSHQVGQTGTTVKPKIYFACGISGAIQHLAGMQTSDMIV
                     AINKDPDAPIFEVADYGIVGDLYKVIPKIIEEWDKAEELYDASTKQ"
BASE COUNT         7593 a         3921 c         5375 g         5121 t
ORIGIN      
        1 agaagatgaa aaaatcgtgt tgttttttga ggcggctttt acttacgggg gatgaatgct
       61 aaactggctg atataggttt gaatgatccc ggtaaagtgg aaatattccg gcaaaaaaca
      121 gacaaatact aaacagataa atccggtaaa acaggaagaa ccggtaaagc aggaagaaac
      181 gaggagaata aatgaaaaaa ttcggatgga tacttttttt gacatctttt gccgcatcgg
      241 ctgttttgag cataattctg gtggcaggga cttttggtgc atccggagct gtgctctcgg
      301 cactgctttt tccggtgaca gcttatgttt acccgattta cgcagcagcc accattggag
      361 gatattattt tatattcccg gtggtttttt atgttttaca gatagttgca tttctaatat
      421 gctcttacgg atacgggagt gaaaaacttc cgggaggcat tgagaccgaa gatgaatatg
      481 aagcaagggg ctttggaaaa gctgccggcg gaatacttaa aaccggagga atggttttga
      541 tttttgtaac tctttcggtt ttactgggag cctttacaag agcagcaata atagacggag
      601 gggtcacctg gcttttgccg gtggcggcta tagcggctgc ggccctcgtt atatgcatac
      661 cgatgagaat gaaaaatggc agcaggtaca acataaggaa ccgcaaaaaa agagacctga
      721 taatagctgt ggtactggct gccgtatttg cagtatccgc ctatttcgga tatgactatt
      781 ttttcggtcc ggcttacagt tcttatgatc tttcaaagta tataaaagtg ggtcagtaca
      841 aaggacttga aaaagagcag acctcaataa aagtaaagga ttccgaagta aaggagaaaa
      901 tagatgaaaa ccttaaagcg gcagcaacca cagaagagac agaaaaagga aaagttgaaa
      961 aagacgatac agtcaatata gattatgtgg ggaaaataaa cggcaaaaaa tttgacggcg
     1021 gctctgcaga aggctacagt ctcaccatag gttcaggcac ctttatcgat ggattcgaag
     1081 atggacttat cggcaaagaa atcggagacc ccgtatcttt ggatcttact ttccccgaag
     1141 attattcgga tgaaagtgtg gccggcagag atgtggtatt caaagttacc atcaattcaa
     1201 aagaagtaac agttgttccg ggacttaacg aagaattcgt acaagaaaac agtgacagcg
     1261 atacagtaaa ggaatacaag aaagagataa aagacaaact ctataaacaa aaagaaaaag
     1321 aggttgccga agagcagaaa acaaaacttt ggaaccaggt ggtggaaagc tctgaagtat
     1381 taaaatatcc caagagagaa atagaatctc tgcagaatga tctgataaat gaatataaag
     1441 attatgcaaa gcagtatgag atggaattca aggacttcct aaaacaatat gccggtatag
     1501 aaagcatcaa agagttcaag aaacaggcaa aggaatatgc caggataata atcaaggaag
     1561 aaatggtgct ttattatata gccgataaag aaggtattac cctgtctaag gaagaatatg
     1621 atgcattcgt tacaaaaact ttgaagactt acgggtacac aagagaacag tttgaagaag
     1681 ctcaggggca gagttatgaa gatgctgtag gaaagagcgt tatcgaaaga cagatatata
     1741 tggagaaagt aaaagatttc atattctcaa aagccaaaat aacaggagaa gagcaataag
     1801 atagtcaaag caaaatatag tttttcttat taaaaaacat tgtatggggc tgcaatttgt
     1861 ggtagtatcg gtacgttagt tatcaatcgg accttgattg cagccttttt tttgcgcaaa
     1921 agcaaacaaa gacaagagga aacaaggata agaacgaaac tacgattctc tggagagtcc
     1981 catcaagggg cgccgaaggt ggaggccggt catgccggtg agtctctcag gcaacaggac
     2041 agagcatagt agcgatgtct tctctctgga aagtcgaaat tatcggcttt ttttatttat
     2101 ttttcaggaa aagaggagaa gcgaaatgga acaatttacg aacatactgg tcagtattga
     2161 tgatgtggta tggggagtgc cccttatcat actcataatg gggaccggta tattactcac
     2221 ggtaagactc ggggttttac agttcagaca tctgggcaag gcacttaagt atatgatcca
     2281 caatgaagaa ggcggaaagg gagaagtcac cagtttcggc gccctttgca cggcaatggc
     2341 agctacggtg ggaacaggaa acatagtggg agtggcaaca gccataattg ccggcggccc
     2401 cggagctttg ttctggatga tccttgcagc atgttttggg atggcaacaa aatattcgga
     2461 agggcttttg gcagtaaaat accgtacttt ggatgaaaac ggacatgcat tgggcggacc
     2521 tttctactat atagaaaaag gtatgggcag tcggtggaaa tggcttgcca aaacctttgc
     2581 tttcttcgga acggctgccg gacttcttgg cataggtacc ataacacaag taaacgggat
     2641 agcatctgca acaaaaactt tttttgatcc ggaaaacaaa aatattgctt tcagcatagg
     2701 caatatggaa tattcgtggg taacagtaat aacggcagtt gtggttacgg tgctggcggc
     2761 actggttata ataggtggta taaaaaggat cgccagggtt gcaacagtta ttgttccatt
     2821 tatgataatg gcatatgtga cagtatgcgt atcaatactc atcgtgaatg tggcagaaat
     2881 acctgaagct gtagggactg tatttgccag tgcctttgga gtaagagccg ctgccggcgg
     2941 cgctctggga gcaatgatga tagcaatgca aaaaggtata gcaagaggaa tattttctaa
     3001 tgaatcaggg cttggaagcg cacccatagc agcagcggca gctaaaacaa acgagcccgt
     3061 aagacagggg ctggtttcca tgaccggtac gtttatggac acgataatag tatgttcaat
     3121 gaccggtctt accataatgg tgacaggagc atgggaaaga gggctggaag gcgtagatgt
     3181 tactataaat gcctttgaga caggtcttcc ttggaatagt gggacaggag catttatact
     3241 gatgctgagc cttgcattct ttgcttttac cacgatactc ggatgggatt attacagtga
     3301 aagatgtctt gagtatttga ccggaaaaaa tttaaaagcc ctttcggttt tcagatggct
     3361 ttacatactg gcagttctga tcggaccctt cctgaccctt tcttttgtat ggacttttgc
     3421 agacattttc aacgggttaa tggccatacc caatcttata gcactggtgg ttctaaacgg
     3481 tgtagtcgtt gccgaaagcc gaagttattt caaaagactg aatacaggac agcttgatga
     3541 cggcttccgg aaaaaatagg tttctgataa tcaagagctg tttatggtat catcgtgaac
     3601 agctctttta ttacagtatt tttacattgt ttttcttgtt agataccaca atatgtagta
     3661 aacttataaa gataagattt ttttagtgta tttgagggag gtcaatcata tatgaggtgt
     3721 ccgttttgtg agaatgaaga tacaaaagtc attgattcaa ggcatacaga agaagggcat
     3781 gcaataagaa gacgcagaga atgtgagaaa tgcggcaaac gatttacgac ttatgaaaaa
     3841 atcgaagaaa tcatacttat ggttataaaa aaagacggca gcagagaagc atttgacaga
     3901 agcaagataa tgaacggtat aatcaaagcc tgtgaaaaaa gaccggttcc tctttctgaa
     3961 atagaaaagg tagttgatga cattgaaaga ggtctcaaca atatgatgga aaaagaagtc
     4021 aaaagcactt ttatcggaga gcttatcatg gataatttaa aggagctgga tgaagtcgct
     4081 tatgtaagat ttgcatcggt atatcgccag tttacggata ttaatacttt tgtggccgaa
     4141 atagaaaatc ttctcggcag caaaaaaaag aagggaactc aaaattgaag gatattttca
     4201 gggtggccat tgacggtccc ggcggcgccg gaaaaagtac tgttgcaaaa gaagttgcaa
     4261 atgcgctggg catagattat atagacacag gagccatgta tcgtgccgtc ggatataaaa
     4321 taataagaga gggcatagat ccggacgatg aggaaactgt cgagaagatg ctttcaaaaa
     4381 cagacataga ttttgttaag ggagacgtaa tgcttgacgg agaaaaggta aatggtcgga
     4441 taaggactcc cgaagtgtca gaaatggcgt ctttatgttc tgcttattct tttgttcgca
     4501 aaaaacttgt ggcagcacag agaaaaatgg gagaaacaaa aagtataata atggacggaa
     4561 gagacatagg aacaaacgta cttgccgatg ctgaatataa gttttttgtt accgcttccc
     4621 cggatgaaag ggcaaagcgc agacaaaaag aattatctga aaagggcgaa gatatttctt
     4681 acgaagaagt actgaaggac ataatcaaac gagatcataa agatacaaca aggaaactga
     4741 atcccctgaa acaggcggag gatgcaatac ttatagatac cacgggaatg actgtagaag
     4801 aagtggtaag caaaatactc aaggagataa aataatggct ttagtaagac ctttcagagc
     4861 aatacgacct aacagacaat ttgcggaaaa ggtggtttca ctgccctatg atgtgatgaa
     4921 cagagaagaa gcagaggcaa tggtaaagga taatccatac agtttccttc atatatccag
     4981 atcggaaata gacctgccgg aacagaaaga cccctatgct cccgatgttt atgaaaaagc
     5041 aaaagaaaat ctgcaacagt ttcttgacaa gaagattttt attgaagaag ccaaacccat
     5101 gttctatata tacagacagc aaatgagaga caagatacag accggtcttg tgggttgcgt
     5161 ttcggtggac gattatttca atgacataat aaagaaacat gaacatacga gagaagaaaa
     5221 agagctggac aggacaaggc actttgatgt gtgcagcatg cataccgaac ccgtatttct
     5281 ggcatacagg gacaacaaaa aaatcgacac gcttatggaa ggttatataa caaataaccc
     5341 ggctgaatat gatgtgacaa catcagatgc tgtcgaacat aagctctggg caataaccga
     5401 tgataatgtt attgccggac ttgccggaat ttttgcggaa ataccggcac tttatattgc
     5461 cgacggacat cacagaagcg cttccgcctg ccaggtggga aagaaaagaa gagaagaaaa
     5521 accggattac acgggagaag aagaatttaa ttatttcatg gctgttatat ttcctgataa
     5581 agatcttaat atacaggatt acaacagagt tataagggac ctgaacggca atacatctga
     5641 agaaatgata aataaaataa aagaagcggg atttgatgtc gaagaaaaag gtacaaaaca
     5701 gtattttcct gaaaaaaaac acatattttc catgttcctt gaaggtagat ggtataagct
     5761 aactgcagaa tcatcggtca taccaagaca tataacagac tcactggatg tttctgtgct
     5821 gcaggacagg atactgtcac ccatacttgg tatagaggat cccagaaaag ataaaaggat
     5881 agactttgta ggcggtataa gaggacttga tgaacttgaa agaagggtaa atgacgatat
     5941 gaaaatagcg tttgcattgt accctgttat gatagaagac attatgaaag tatcggataa
     6001 tgacatggtg atgcccccta aatccacctg gtttgaacca aaactcggca gtggtctttt
     6061 cctccacagg atatagaaaa catgttgagt gaaacggagc ctttttcttg tatagagttg
     6121 taaaaaatgt taaaatataa cgcagttttg agaagaggga aataatggag atgccggata
     6181 agaccttaat ggtacataag taccggaaag gaaacggcat tatgatacta agagaactac
     6241 cggaaaacga aagacccaga gagaaactga tctacagagg caagggttcc ctgagcaatg
     6301 cggaactcat cgcaatactc ataaggacag gcagcaggga catgtctgcc ctgcagcttg
     6361 cggaagaact gctttccatg gacaaaaacg ggatattttc cctgggagaa tgctctgtgg
     6421 aagaactttg tgcagtgaaa ggaatcggaa aagctaaagc ctgtcagata tcagcagcaa
     6481 tggaactggg aagaagggta gcagccacac ccagaaaaag cagacagagg atcaagggca
     6541 gcagagatat agcaggaata tacatggaag aaatgcgtta ctgcaaaaaa gagtatttca
     6601 atgcggtaat gcttaatgca aagaaagaga taatagcgtc tgaaaatata gctataggag
     6661 atctttgttc cagcatagtc catcccagag aagcctttct ttgcgcagtg aagcgaagcg
     6721 ctgcgggggt catattcatg cataaccacc ctagcggaga tccaagaccc agcaaagagg
     6781 atatcgatgt gacagagaga ctttgtgaaa ccggccggat acttggaata agtgtacttg
     6841 accacataat aattggagac ggaacttata tcagcctgaa agaacaggga ctgatatgaa
     6901 atattaacag aaaaaatgta tggaggaata gcaaagtgat cagtttttca tcaagagata
     6961 tggggataga cctgggaaca gcaaacacaa ttatttacat gaaggaaaag gggataatat
     7021 tgaatgaacc ttccgttatt gcctatgaca aaaatattgg ggagataatt gccaccggcc
     7081 agaaggcaaa ggatatgtac ggcaagactc ccgccaacat aatagtagtc aagcccctgc
     7141 aggacggagt gatatcagac tttgatatga cctcggagat gctccagaga ttcatcaaaa
     7201 gatctctggg agaaagaaaa ccctcgggat tgagagtggt ggtcggagtg cccagcggtg
     7261 tcacagaagt tgaaaaacgt gctgtggaag aagtggtaag acagatgggg gcaagagaag
     7321 tatatatact tgatgaaccg acagctgcgg ccattggggc agggctcaat attgatactt
     7381 cagaagcttc aatgatagcg gatatcggcg gaggaacaac tgacatcgca atacttgcaa
     7441 tgggaggtat agttgtcagc acctctttaa gatatgcggg agataaattc aatgaggcgg
     7501 taatacaata tataagaaag aaaaaaggag tactgatagg tgacaagacg gcagaagagc
     7561 taaaaataaa tgttggaagt gcccttgttg ataaagacac ctccggtgag gaaaatctct
     7621 attcaatgaa agcaagggga agagatctta tttcaggtct tcccaaaact tttgaagttt
     7681 ccaacaagga tatggaggcg gccctttatg agtccatgga gcttataata gatgcactaa
     7741 aatccacggt agaaaaagca ccaccggaga tcgcagctga tatcgcagaa aaaggaatgg
     7801 tcctttccgg cggtggtgga atgatagaaa accttgataa actcataagc cagagaacag
     7861 gaatgatagt agagaaagca aaggatcccc aggaggcagt tgccttggga acaggcaaaa
     7921 gtcttgataa tatagagaaa ctgagaaaat acgttgatgc caagaaaaga tagggaaacc
     7981 aaaaatgaaa tggataaggc agcataaaat tttttctgtt acagccgtta tagttattgc
     8041 tttatgttta tttgtgctgg gctcatatct ggcaggcggc ggttcgggcg tgctgggccg
     8101 tggggtgcag agtgtgatag cagcaataga aaaacctctt tcggctttta ccggcggagc
     8161 aagggatact ttcaaaggga tattcagatt cagtagtgta cttgaagaaa atgaaaatct
     8221 aaaagaagaa aatgctattc tcagacagga aaatattgat ctttccatga aaaaagatga
     8281 gcttgaccgg ctggaggaat tgtcaaaagc atttgatttt gaaccatatt cgggacaggg
     8341 caaagctgtg gctgcaagga tcatatcact gaacaattca aacacatatg atgttttcac
     8401 cattgacgca ggatcttcaa aagggatcga gaaaaacgat atcgtcgttg acggagaagg
     8461 acttattggg aaagtcaaag aaaccggaga cagctgggca aaggttgttt cagtccttaa
     8521 cgaaaacaac agcataagtt tcaaagtcca gagaaaaact tccattacag gaatagttgc
     8581 cggagacgga aaaggaaaga tcgcggggta ccttatggac aacaaggcta caatcgtcaa
     8641 aggtgatgtt ctggtgacat cgggcatagg catatatccc gaaggtataa gaataggaaa
     8701 agtcacttcc gttgattaca acgatgacat gcaactgaaa gaaataactg taaagtcaac
     8761 tgtcaaattc acaagtcttc aaaaggtggc ggtctttaaa tgagttataa aaaggccgtt
     8821 ttactgtttg cttttgcatt tataatgcag ctttccatta tcaatctgat aaacatcaat
     8881 gggatcgtac ctgaccttac tctttgcctt gtaataatga taactttttt gtttgaagac
     8941 ggcttcagat acggtgccat aggcatcata agtgctctcc tgctggattt gtgcaccggt
     9001 cagtatgtgg gcataagttc tcttggaatg atcatagtta tggcaggaat agtactttta
     9061 tgcaggagca tgaaccggga aaacaaactg tcttttgtca tagtaactgc tgcaggaact
     9121 cttatatatg gattgttact ctggcttttc atggccatgc tgggtgatcc cacaggcata
     9181 ttgtatgtac tgatgtatca gcctttttat atggcataca acatcgtatt ttcatttatt
     9241 ttgtatttgc tcatgataaa aaaagcagtt agacacagaa atgacaggta ttttgtatga
     9301 atgattttct gtccaggtac aataaaatac tttttttgct tctggcgctt gccattatac
     9361 ttttggtaag gctttttatc cttacggtaa tacaaaacaa tgactggtcc gaagcagcgg
     9421 agggaataag cacgagaaat atatccacca cggctcccag aggagaaata tatgacagaa
     9481 acggtaatct tcttgccgga aacaaacaga cctttacagt aaaaatgagt gccgaagata
     9541 tgaccgataa agagcttaac agcgtaataa caaagcttat tggcatcctt gaagataacg
     9601 gcgacagttt tgaggacaat ttgcctataa aagcaaaaaa cggaaactac tattatactt
     9661 atgaaacaga aataaagaaa tggcttgaga aaaagggatt tccccaagac tatacggcgg
     9721 aagaggcatt ttttgccctt agggatcagc tgggaataga taagcagctt gacagatatg
     9781 atgtccagct tgaaatgcag aatatgtaca atgaatatcc gccgatttcc gttacaagga
     9841 tgaaatataa tccggaaata gagaaagagg aatttctgga gagttacggc ctcagcaaaa
     9901 atgtcaacgc tgaaaaagct ttcaaagcca taagaaaaga aatggatata tccccgggaa
     9961 cgtcggaaaa agaagccaga aaaatcatgg ctgtaagaaa tgagcttgaa atgctgggat
    10021 ataaaaaata tatgccggcc actatatcaa aaaatgtatc agacgataca gttatgataa
    10081 ttgaagaaaa cagcagtgaa ctgcaaggtg tagaaacagc atcggaaaca acaagacatt
    10141 atcctgccgg caaatccgca tctcatatat taggttatat gggtaaaata tccgaagata
    10201 aagaggaaga atacgaagag aaagggtatg aaaaaggtgc ggtaataggg ctttcgggaa
    10261 tagagggcaa atatgaatcc attctcaaag gaatcaatgg cgcaagaaaa gttcaggtca
    10321 acgcatatgg agatatggtc aaagagataa gcgactcgga accggaaaaa ggcaaggaca
    10381 tatatctcac actggatctt gatcttcaga agtctgcaga gagatctctt gagcagggac
    10441 ttgccgcaat aagggccggc ggaggattca aaagcaaata tggcagtgca cccatttcaa
    10501 aagctgcccc ggcgcaggtg ggagcggtgg tcgctctgga agttgaaacc ggagatgtgc
    10561 tggcaatggc cagttatccg gattacgacc ccaatctttt tgctgaagga ataagcgaaa
    10621 aagactggga ctctctgcaa agcgaaaacc caagagatcc acttgcccct tctcctcttt
    10681 ataatgtggc aacacaatca gcagtgcagc ccggttccac attcaaaatg gtaactgcca
    10741 ccgcagggct tgaatgtggt cttaatccct atagactgct cagagacgga gggcatatag
    10801 aaataggaaa caagactttt gcctgtgttg tatggaatat gagcaaaagc acccatggct
    10861 atgtggatat ggcagatgcc ctggaagctt cctgtaacta ttatttttat gatgtcgcta
    10921 cagggaaaga ctgggccgac ggaggctctt taggatacaa aaaaaagata acagtagata
    10981 caataacaga ttatgcctcc cagtacggac ttggtgaaga cacaggcatt gagatacctg
    11041 aggcagtgat acctgtacct tcagaggaga gaaagaccga taacctgaag accagcctta
    11101 aaaatgttct atatgccgca gctgaaacat attttaaacc taagatcgta aaaaacaaag
    11161 aacttctgga ggagtcaata gaaaaaataa taagctgggt gggccgggac atagggcgcg
    11221 cagaaatgat aaacgaaagg ttgcctaagc tgggagtcag aaaaagtcag atagaacctt
    11281 tggcagatct gtgcctttac acatattata atcaggcgga ctggagtgtg ggagatgcca
    11341 tgaatatcgc cataggacag ggagaaaatg catatacgcc tctgcagatg accaattatg
    11401 tggccaccct tggaaatgca ggcaaaaaac acaaagtgag tgttgtaaaa agtattgaag
    11461 atgagggaca gactgaagag gaacagactg aaaaagtccg ggtaagcagc gataaatatt
    11521 tcgaccagat aatagacggt atgaagcagg cggctgtggg atccagagga agtctgggag
    11581 cgcttttcag gaatttcccc gtggaagtag cggccaaaac cggtacagcc gaaaggggag
    11641 gatatgtccc gcccaaatcg gaagtcacat atataaagca gcacctttat gcatttgaca
    11701 gttcactgag ctggagtaaa ataaaaaaag agatgaaccg tcttatgaaa gaatatcccg
    11761 aaacatacac aaatgagaat acagctgtaa gacgtgcggt tataaatcta agtaaaagaa
    11821 aaataaccac agagacactg gatcagttca agtcaactta tgacaatttt gcatgggtgg
    11881 tagccatggc tcctgcagat gatcccaaga tagctgtctg tgtaatggta gtgcagggag
    11941 gtacagctgc caacgccgga cccatagccc gggaagtgat aggcgattac cttaatgtaa
    12001 acggtgaaag cattgaatat aaaacatata ataatgaaac aggtattcaa tagaaaggaa
    12061 aacatggatg agcagatctg ttctgatagc atcgggaaaa ggcggcaccg gtaaaacttt
    12121 ctttgctgcc aatgcaggag cactgctggc gatgaaagga cataagacag ttatactgga
    12181 tatggatatg ggacttcgcg atcttgatct ttatatgggt cttgaaagca aagttgttta
    12241 taatatcatg gatgtacttt ccgggacatg caaaataaag caggccctaa taaaagacaa
    12301 gcgtttcagg gatctttata taatggcggc agctacggca aatgacagaa gagatgtgac
    12361 acccctgcat gtaaaaatcc tttgcgaaaa actgaaagaa cgttttgatc atataatcat
    12421 agatgccccc gcagggatcg gagaggggct ggaactggct gcggcaggcg ctgatatggc
    12481 ggtggtgata actgaacctg aaattgcatc ggtaagagac gcagatgctt tggacagaaa
    12541 aataaaagat tttgggatag ctgaaacata ctgtgtggtg aacaaggtga acggagagct
    12601 gatgacatcg ggggttgtac cggggatatc gaccatatca aaaggtctaa ggatggaaat
    12661 agccgggatg atacagaggg ataacaatat aaatatttcc acaaacaaag gagaacccat
    12721 agtaatgaaa aagggaacgt atatagaaaa aaatatggca aagataatcg aacggatact
    12781 ggattgaaaa tcaggcgaaa gcctgatttt tttatgtttt atatcaaaag gtcacaccac
    12841 ggttccttat ggccacattt attatgaagc cggtggccac catgcacgaa agaatagatg
    12901 tgcctccgta gcttatgaat ggaagagtta ttcccgtaca gggcattaca cccatgcaca
    12961 ttgcaatatt ttcgaaaatc tgaaaaacga acatgcccaa aaatcccata actaccaggg
    13021 ttccgtaaag atcctttgcg gacaaagcca ctttggatat ggcatatatg aaccaggtat
    13081 atgcggcaat aagaactgca ccgcctataa agcccagttc ttccacaacc accgagaata
    13141 tgaaatcgga atttcgaaca ggaaggaaat ccagatcctt ttgtgtcccc tgaaaaagcc
    13201 ctttcccgaa gaaaccgccg gagcctatgg cgacctttga ctgccatacc tgatagttgc
    13261 cctggagagc cagattgtcg ggatggatga atgcagttat cctctcctgc tgataatcgt
    13321 caagcagact gtaagcagcg ggaataaaaa gcaaagtaat acctgcgcat tttgcaaaaa
    13381 gcctgccgtc tataccggca aagaatatca ttacgatcca catgattatg aagaccacag
    13441 cactaccgaa atcctctttc aggacaatga gtataaaagg tgcggcataa agcgctgcca
    13501 tgagaatgct ttttagagta taaagtttat cacggttgcg gttaaggtag ttggccatca
    13561 aaagaacaaa agtaattttg attatttcag agggctgcag ggttgtgatg ccaaggttta
    13621 tccaggatct ggcaccattg acctcaaggc ctataaaagg cagatagggc agaagcagaa
    13681 atactactga gcctatataa agaggtcttt ctatttcctc aaatacggaa tagtccatac
    13741 tggctattat gataataaga acaaagccga tcagatatgc agctgactgg acaatgacat
    13801 cccttgaaaa cactatatgg ttatcgtatg aagtgctgaa catcatcaat atgctcagga
    13861 tgcagaagaa aaccggaaga agtatcaacg gcttgctgac atttttgaaa agatcatgga
    13921 aattgtctaa tttcttcatg ctttgccctt cgtttacctt gacataataa caataaaaac
    13981 attataatat tacatgtaga ttatatcaac atttaattcg gtaatgcgtg ttaaatatat
    14041 taaaatcaag cttggcgttt gaataaatac aacagaatat cggaaggagg tgttatttaa
    14101 tgccagacat agttttagct gtaatagttg gactggttgc attggtgatc ggcattttag
    14161 ccggctacat catccgaaaa actttaggcg aaaaggccat tggaagtgcg gagcaaaagg
    14221 ccaggaacct gatcctggat gcagagaata aatcagagac cataaagaaa gaaattgctc
    14281 tcgaagcaaa agaagaagcg cacagactgc gaagtgaagt tgaaagagag ataaaagaga
    14341 gaaggaacga aatttccaaa tcagaaaaaa gactcataca aaaagaagaa acaattgata
    14401 agaagcttga aaacatagaa agaaaagaag acagcattac aagcagagaa cagaatataa
    14461 tcaacaaaca aaaagagctg gaaacacaga taaaaaaaca gaacgaagaa ctggagaaaa
    14521 tctccggata cacggccgaa gaggccaaag ccatattgct ttcaaatctt gaaaaagaaa
    14581 taagagtaga ggcttctgtg cttgtaaaag acatcgaaac aaaagcaaga gaagaaggag
    14641 aaaaaaaggc taaagagata ataacaggtg caatacaaag atgtgctgcg gatcatgtgg
    14701 ctgaaagcac agtatctgtt gtacccctgc ccaatgacga gatgaagggc cggataatag
    14761 gtcgtgaagg acgtaatatc agagccattg aaactctgac gggtatcgaa ttaataatag
    14821 atgatacacc ggaagcggtc atcttatcgg gctttgatcc ggtaagaaga gaaatagcca
    14881 gactgtctct ggaaaaactg attatcgatg gcaggataca tcccgccagg atagaggaga
    14941 tggtaaacaa atccgaaaag gaagtaaatg catctataaa agaggccgga gaacagtcaa
    15001 cctttgatgt gggagtgcac aacctgcatc ccgaacttat caagtatctt ggaagactca
    15061 aatacagaac ctcatacgga caaaatgttt tgaaacattc tgaagaagta gcttatcttg
    15121 caggacttat ggcaggcgaa ctgggctttg atgtaaaact cgccaaaaga gccggactgc
    15181 tacacgatat cggcaaggct gtagatcatg aaaaagaagg gacccacgtg gaactgggca
    15241 tcgagctttt gaagaaatac aaggaatccc aggcgatcat tgatgctatg gcttctcatc
    15301 acggtgatta tgaaccaacg accatggaag cggtgcttat cgcagcggcg gatgcattgt
    15361 ctgcggcaag accgggagcc agaagagaaa ctctggaaac atacattaag agactccagg
    15421 ctcttgaaga gattgcaaac aacatggatg gagtggacaa atcatacgcg atacaggcag
    15481 gcagggaaat aagaataata gccaagccgg atcgggtcaa ggatgaggaa atgcctttcc
    15541 tggcaagaga gatatcaaaa aaaatagagg aagagctgga ctatccggga cagataaaag
    15601 taaacctcgt gagagagacc cgggcagtgg attacgcaaa ataagagttt tgaatttgat
    15661 ctgaaagggc agttttactg ccctttcttt aataagagga gcaaaggcgc aaagaaacgg
    15721 ggaacaggag aaaaaatgat agtatacctt gataacagtt caacaacaaa acaatatgac
    15781 gaagtgacag aaataatgtt caaatgcatg caggaggaat acggaaaccc ctcatccctt
    15841 cacaggatgg gattcgcttc tgagaaaata ataaaaaaag ccagagaaca ggtcagtgaa
    15901 gccctttccc gttcagggct gtgtgccgcc gctgaaggag aactcttttt caattcggga
    15961 ggtacggaat cagataatac agctgtaatt ggggctgcaa aagcccgcag aagatatggc
    16021 aacaaactga tcacatcgga agcggagcac cctgccgtgc ttgagtcatt caaaagactg
    16081 aaagaagaag ggtttgaaat ttccctcata agagtagatg aaaactgcag gatcgatatg
    16141 gagcagctaa aatctgaaat aaacgagaag actatcctta tatctttaat gaaagtaaac
    16201 aatgaaacgg ggactataca gcccatactc gaagcggcag agcagaaaaa gaatgctttg
    16261 tttcacaccg atgcggtcca ggcattttgc agagttcccc agtcggttca gaatgcagac
    16321 atgatatcaa taagctcaca taaaatacat gggcccaaag gtgcgggagc actatggata
    16381 aaaaagggta caaacataaa gcctttgata ctgggaggcg gtcaggaaaa caaatttcgt
    16441 tcaggtacgg aaaatgttcc ggccatagca ggtttcggta tcgcagccgg gaaagcatca
    16501 gatggaatgg aagaaaaaat gaaaaggatg tcagacatcc gaaaatatct gcttgaaggg
    16561 ataaaactgg aaatagcgga cataagcata aacagtcctg tttattcatg ctatgagaaa
    16621 aagcaggaaa ctggtgaggc cctttgcagc ccttctattc tgaacatttc ttttctcggc
    16681 acaaggggcg aagtgatcct tcacagactg gaacaggagg gtatttttgt ttccacagga
    16741 tctgcatgtt cctcaaacaa gaagggcaaa agccatgtcc tttccgccat ggggctgtcg
    16801 gagaaagagt ctgaaagcgc aataagattc agtttgagtg aaaccaatac gattgaagaa
    16861 atggatcatg tcgtgtataa tcttaaaaaa gcagttgata aattcagaaa actcgggagc
    16921 ttgagatgaa cgaaaaaaac atttatatca taagatgcgg agaaactgcg cttaaaggtc
    16981 agaacaaacc atattttgag cgtatgctgg cggacaggat caagaagata ctcaaaaagt
    17041 ttcccggcat agaagtgaaa aggcatgagg ggcttatttt tgtgaggaca gaaaaatcaa
    17101 ctgcggagaa agacatactg aaagagacag gaaaagtttt cggcattgcg tccataagtc
    17161 ctgctgttga ggtggaatcc gatatggaga aaataggcac ggcagcagtt tcatatatga
    17221 atgacctgat aatgaaaaaa ggtataagaa cattcaaggt cgaggcaaag agagcagaca
    17281 aatcattccc tgtaaaatca ccggacatag caaaaaaaat cggagcggcg gtcctaagag
    17341 gatgtgctgt tcttagcgtt gatgtgcacg atcccgatgt acatcttttt gttgacctaa
    17401 gacatgacaa aacatatata tatcagcaga aaataaaagg tttcggtggc cttcccctgg
    17461 gcaccaacgg aagaggcatg atattgcttt ccggggggat agacagtccc gtggctgcat
    17521 ggatgatggc aaaaagaggg atgcagatag aggcagtcca ttttcattca tatccttata
    17581 ccggtcaaag ggcccaggaa aaagtggctg aactggcgtc gatcctggcc atatactgca
    17641 gcaggttcaa attacatatg ataaatctgc ttcctataca ggagcagata gtgcagaatt
    17701 gccccgaaga ggaaaccaca gtgctgctga gacgcttcat gatgcgggta gcagaaaaag
    17761 tcgccacaga tgccggatgc ggcatgctta taacagggga aagcctgggg caggtggcca
    17821 gccagactgc cgatgctata atagtaacgg atgaagcagt agaaatgcct gtgatgagac
    17881 ctctgatcgc catggataaa gtggatatca tggatattgc aaaagaaatt gaaacatatg
    17941 aagtctctat aatgccctat gaagactgct gcacagtttt tcttcccaaa catcccgtaa
    18001 caaggccaaa gatagagagg ataaaaagat ccgaagaaag gctttgcgta gacgaactca
    18061 taaatgaagc tttaggctca gaagaaatac gtgatatata cccaaaataa aaaagcggga
    18121 gatcccgctt tttttattaa cggatctgat agaaagcgtc aaatcacgag tgcctttttt
    18181 tatattttgt gaaacaaaaa gcaagcgcag tgatgccggc ggcaaaacca atcgaaaata
    18241 tgactaaggg caacttggat ctatttttca taaatacctc cttgaaagca atgtatcatg
    18301 ctcattttta ttgtaacacg gcggctgcaa aaggagcaag acaaatgttt gaaaagtcag
    18361 aaatttccgt taaaaaacgt tggaatttca gcaataatta tattagaact taggtgaaaa
    18421 aaataataaa atccacttgc aaaagtaaca aaaacatgtt ttacttatta tgttatattg
    18481 ttagaaatat aacaatatat tttggcaaaa atacggtggt atattcaaga taaaataact
    18541 aaccgataaa aatatttaag gaggcaatta catgaacttt caactgacta aagaacagga
    18601 atttgtgcgg aagatggtaa gagagtttgc gacaaatgaa gttgaaccga tcgcagccga
    18661 tatcgatgct gaacacagat ttcctgaaga gaatgtaaag aaaatggcag attacgggct
    18721 tttagggata ccgttcccca aggaagtagg aggggcaggc ggagaccagc tttcatatgc
    18781 aatagctgtg gaagagcttt caagagtatg cggatctaca ggggttatat gctctgccca
    18841 tacatctctt tgctgctggc ccatatatgc atggggcaat gaagagcaga aagcaaaata
    18901 cctgccggat gtctgttccg gaagaaagct tggtgctttt ggcctgacag agcccaatgc
    18961 gggaacagat gcgggcgggc agcagacaag agccgagctt atcggagacc agtgggtgct
    19021 taacggtgca aaagtattta taaccaacgg cggatatgca gatgtattta ttataatggc
    19081 tatgaccgac aagaaaaaga aaaccagagg aggaataagt gcttttatag tcgaaaaaga
    19141 cgatcccgga ttttcaatag gcaaaacaga ggataagatg ggcatctgtg cttcttccac
    19201 aacagaactg attttccaga actgtactat tccaaaggac agacttctgg gacaggtagg
    19261 agacggattc aaagttgcca tgtccactct tgacggaggc cgcataggca ttgcatccca
    19321 ggcactggga atagcacagg gagcatttga tgtgactgtt gaatacatga aagccagaaa
    19381 acagttcggc aagaaactat cccagatgga agctctgcag tttgaggtgg caaatatggc
    19441 aacacagata gaagcggcaa ggctgcttat ataccaggca gcagacatga aggaaaaaaa
    19501 tctgccctac ggaaagtatt ctgccatggc aaagcttttt gctgcagaaa ctgctatgta
    19561 cgttacaacc aaggctgttc agctccatgg cggatatgga tacacaaaag actatccggt
    19621 ggagagaatg atgagagacg ccaagataac tgaaatatat gaaggtactt cagaagttca
    19681 gaagatggtt atcgcagcat cggttttcgg taaataaaga caggaggaaa acgaaaatgg
    19741 catttaaaat aatcgtatgt gcaaaacagg tacctgatac aaatgttatc aagatcaatc
    19801 ctaaaacagg cacattgatc agagaaggcg ttccaagcat cttgaaccat gacgatgcaa
    19861 atgcgctgga agaagctttg aaaattaaag ataaatatcc cgatactcat ataacggtgg
    19921 tatccatggg tccgcctcag gcaaaggaca tgcttcttga atgtctggcc atgggagcag
    19981 acgagggcgt tcttgtcagt gacagagctg tcggaggatc cgatacatgg gcaacatcca
    20041 atgcgattgc cgctgccata aaaaagatcg gtgagtttga tcttatcttt gcagggcgtc
    20101 aggcgataga cggagacact gcccaggtgg gaccccagat cgcagaaaaa atggatctgc
    20161 ctcaggtcac atatgttcag gaatttgaaa tagaaaaaga tatgaaaagc ataacagtaa
    20221 aaagagcgtt ggaagacggc tatgaagtta taaagataaa gacgccatgt atgctgacag
    20281 cgataaagga actgaatgct ccaaggtata tgaccattgg gggaatattc ggtgccaatg
    20341 aaaaacccat acatgtatgg aatgcaaagg acatagcaat tgatcttgat actgtaggtc
    20401 ttgaagcatc tccgaccaac gttttccgtt cattcactcc tgcccccaaa ggcaagggcg
    20461 ttatcctgga aggcgacacc gaaaaggaag ttgcagaaaa acttctgata ggcctaaaag
    20521 gcaagcatgt agtataagaa ggagggaaaa caaaaatggc aattgagata ataaaagata
    20581 aatgtatagg atgcggcata tgtttcaaat catgccccat ggatgcattt gaatttgagc
    20641 cttatgacgg caataagctg ggcaaggtgg caaaagtaaa ttcgaaatgt actttctgca
    20701 accagtgtct taccgcctgc aaatacggtg caataatcga aaaaaagaca cagggcggag
    20761 aagtggattt ttccgcatat aaacatatat gggtatttgc ggaacagaga aacggaaagc
    20821 tgatgaatgt ggctctggag ttgataggag aaggatacag actggcaaag gaaataagtc
    20881 cggacacaaa agtctgcgct gttatcatag gcgacaaaga tgcaaaggtt gatgcccttg
    20941 aaaaggaatg ttatgagtac ggagcagaaa gtgtcatcat gatacaggat cctttactta
    21001 aaaactatac aacagacgga tatacgaaag tacttactga tgccatccaa gagatgaaac
    21061 cggagatagt tatatacgga gcgacacata taggaagaga tctggctcca agggtggctg
    21121 caagactgga tacgggcctt actgctgact gcacaagact tgatgttaag gtgtcaagct
    21181 atatcgaata tgcaaaaaag aatactactc ttgacacatc cacccttgac ccgaacgatg
    21241 aaagcacagg aatcaaacag acccgaccgg ccttcggcgg aaatcttatg gctaccatta
    21301 tctgtcccag aaccagacct caaatgtcga ctgtacgtcc cggcgtaatg cagaagagag
    21361 aaagagtttc cggtgcaaag ggagagaggg tggactacaa gcccgttata agcaaggacg
    21421 acataaggat cgagataata gacatagtaa aatcggcaaa agaacttgtt tcacttactg
    21481 atgcggaaat aatctgctca ggcggaagag gactgggcga tgcttcggga ttcgagctta
    21541 tacagaagtt tgcagataaa gtaggaggag ttgtgggttc gtcccgtgcc gcagtcgatt
    21601 ccggatggat agatcattcc catcaggttg gacagacagg aactaccgtt aagcctaaaa
    21661 tatactttgc ctgcgggatc tcaggagcga tacagcatct ggccggaatg cagacatccg
    21721 atatgatcgt ggcaataaac aaagatccgg atgcacccat attcgaagtt gccgattacg
    21781 gcatcgttgg agatctttac aaggtcatac ctaagatcat agaagaatgg gacaaagcag
    21841 aagagcttta tgatgcctca acaaaacagt agataataaa agatataaag gatcataaat
    21901 cggtcataag atcatctctg ccaataagat acaattttgg cagggatgat ttttatttaa
    21961 tagggcgttt aagcccgtgt cgagtgcgtg ggcgcacgag ataaaaacca
//
LOCUS       DAONHK010000003        19730 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_2206_length_19730_cov_4.970978, whole genome shotgun sequence.
ACCESSION   DAONHK010000003 DAONHK010000000
VERSION     DAONHK010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 19730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19730
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2206_length_19730_cov_4.970978"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(38..889)
                     /gene="cdaA"
                     /locus_tag="PLM92_00275"
     CDS             complement(38..889)
                     /gene="cdaA"
                     /locus_tag="PLM92_00275"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005999767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HPR23612.1"
                     /translation="MEGFLNNLASDIGVTDIIDILIVAFVVYKILGFIRESRAEQLVK
                     GLLVLVAAFFLSDWFHLHTLNWILKGTMTVGIIALIVVFQPELRRGLEYMGRSKIIKP
                     VFGKVDKVKGKMIVTEFAEALETMSTDKTGALIVIERETALEDICETGTIINADISAE
                     LIVNLFYEGSPLHDGAVIVRGDKLYSAGCVLPLTQNKNLSKDLGTRHRAGIGITENSD
                     ALVLVVSEETGIISIAEDGKLDRFLDIKTVEKILLNVYINKGTEKQVSGLKSAINRLG
                     RKKDVSE"
     gene            complement(918..1439)
                     /gene="raiA"
                     /locus_tag="PLM92_00280"
     CDS             complement(918..1439)
                     /gene="raiA"
                     /locus_tag="PLM92_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013429577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HPR23613.1"
                     /translation="MKVVITSKNVNANDRLKDTIEKKMEKLSKYFSDNIIVNVTLSAE
                     KNRQKIEATIRVRGMIFRAEDVSGDMYEGVDNVVEKLSTQMSRFKTKIQKKHKDNKDF
                     VFADWPEEVQEEIKVVKTKKFELEPMTTDEAILQMEMLEHNFFVFLNMETDSVNVVYK
                     RKDDDYGLLETTY"
     gene            complement(1682..2419)
                     /locus_tag="PLM92_00285"
     CDS             complement(1682..2419)
                     /locus_tag="PLM92_00285"
                     /inference="COORDINATES: protein motif:HMM:NF039185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HPR23614.1"
                     /translation="MKIKENIFEVIFPSNIYCICCGSIIDKSRPYALCDSCIKKFHWA
                     ISKTCKKCGKILEETYKNDYCHDCMDTEHVFRKGYTCSRYGLYERAVMMDYKYNDKSY
                     IGRKLAEIMADRMKREEIIWDMVIPVPVHPSREKKRGYNQSELLAKYFAEKTDIPFDG
                     SILIRNKNTEPMKALDTAERRSNVKNAFCIKKGREKDIIEKEILLVDDIFTTGSTLDS
                     CSLELLKKGASAVDILTFAAGGNLSRG"
     gene            complement(2440..4659)
                     /locus_tag="PLM92_00290"
     CDS             complement(2440..4659)
                     /locus_tag="PLM92_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HPR23615.1"
                     /translation="MTEKKAEIKEIIFHNKENAYTIAYVESEEESFTALGILPGCKKG
                     GTFILRGDFKIHPKYGEQFVFTQSREVLPSSAEGIEGFLASGILKGIGPKAAKAIASK
                     FGEDTFRIIEEEAERLTEVPGIGKKRAGSIAEAFKTHKEFAEMTLYFQQFGISAEYAM
                     KLYRVYGKDTIEAVKENPYRLTEDVFGIGFKKADLIAEKMGVDKDDEFRIKSGIKFVL
                     MHFASSGSTFVPQKELCEKAGELMDIESFLINDVLAAMAFEDEIRIEKIEDRNAVFLM
                     PYFMAEQNVCKRLLNLEKADLKPVSTQIGSLIAAAETDTGIHFSENQKYAIETSISNG
                     VCVITGGPGTGKTTIINGIIKILIKSDMSVAVAAPTGRAAKRITETTGHEASTIHRLL
                     EYYYSESEDAMFFGRNAENPLEQDAVIIDESSMIDLMLMNGLLNAVKPGTRLIMVGDS
                     DQLPSVGAGNVLGDILESEFIYSVKLNEIFRQAAESLIVVNAHRINRGEYPDCNTKDK
                     DFFMIRKKSEREVLASILELCGNRLPSYYKDLDPSRDIQVLTPVRKGLLGCVNLNKEI
                     QSIMNPSNELLSEKVYGEKIFRENDKVMQIKNNYMLKWKMADDFAEGEGVFNGDVGTI
                     KKIDNEFNELTVEYESNKYVVYDFSQLDELEPAYAVTVHKSQGSEFPVVVIPVSWFPP
                     VLSTRNLLYTAVTRAKKAVIVVGSEIRMQAMVDNNSIKERYSGLKARLQGFLDRGSI"
     gene            complement(4765..5337)
                     /locus_tag="PLM92_00295"
     CDS             complement(4765..5337)
                     /locus_tag="PLM92_00295"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HPR23616.1"
                     /translation="MGNGTTIIGDIFSTGIVEIQGEVKGNVNTTEDLEVSGKIEGDIK
                     GKNILLNKASIKGRIDAENDAEMKECTILGDVHASNIILDSRLKGTLYAKEKVELDSG
                     AILIGDIDTKKVAIDMGARVKGSIISKEINVKEDEFDFEVSPPRDLRAPTMENSAAFN
                     SNVTEKNDENKISGTVTDDGKENKDKDIIK"
     gene            complement(5562..6854)
                     /locus_tag="PLM92_00300"
     CDS             complement(5562..6854)
                     /locus_tag="PLM92_00300"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPR23617.1"
                     /translation="MIKLPKEVNNIIKALEKKGFSAYVVGGCVRDSLTGQQPIDWDLA
                     TNAELKDMKKIFPAAKVISEKYSVIRIDHTKDDEDGIIADVATFRKEGPYSDKRRPDS
                     VEFVKSIEEDLKRRDFTVNAIADNPSGKIVDPFEGRKDIEKRLIRTVGDPMERFSEDP
                     LRMIRAVRVAAELNFDIYKKTYEAMVANSGLLSKANIKKIREEFERIITCPNAGKGLR
                     MLAGADLMPAIIGDVAAKLNRRQMQQFSTLCDNIDKTKPVLKRRLGLFYTCFEEKKGM
                     QAIEMLDYDENTKESLKDALLMMEKLHFLRTGVDLKAFLVKYGPKRYEYLDNLSKAQR
                     IVYDLGDAKIISRHELLKIIKENKEPIYIEDLAINGDDIIKAGIAEGEQVGHLLLMLT
                     DVVHKKPKDNNKKTLLKYARKFNKNKLAARTRKVKWLR"
     gene            complement(6851..7828)
                     /locus_tag="PLM92_00305"
     CDS             complement(6851..7828)
                     /locus_tag="PLM92_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005999759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPR23618.1"
                     /translation="MKDIYIATIGDEKEISDFFMVKMIAVKMGSNKKQYLDVLLSDMT
                     GEITGKKWDLADEELEGLSRIEAGDLVKIKAQVTEWNGQKQLKITRIRKGQKEDLQDI
                     KDYIKAAPEKPEEMYDYIFDKAGEISDSDLRKLCLKVLKDNKEKLMYYPAAQKNHHAE
                     MAGLLWHMKRMLMTAEKVCQVYSGLDKDLVAAGVILHDTEKIREIEANEYGISDGYSF
                     EGMMLGHIIQSIKVLDKEMLEMDFSSEKRIMVEHMVLSHHYEPEYGSPKKPLFPEAEL
                     LHYLDMIDARMYDMEKAIEKTMPGEFSERVWTMENRRIYRRIEKEETEK"
     gene            7983..8720
                     /gene="nadE"
                     /locus_tag="PLM92_00310"
     CDS             7983..8720
                     /gene="nadE"
                     /locus_tag="PLM92_00310"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005999775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HPR23619.1"
                     /translation="MFFDPKKTKDEIVVWIREFFQKTGPSCNAIVGVSGGKDSSVVAA
                     LCSEALGRARVIGILMPKGHQEDINMAHKLISILGIKSYTVNIEDCYKGLMCHLENEL
                     PEITESTRINLQPRLRMATLYAASQSLNGRVANTCNLSEDWVGYSTRYGDSVGDFSPL
                     ANITVGEVLALGKELKLPDDIVNKVPVDGLCGQTDEEKLGFSYKMLDEYIRTGVCEDT
                     ALKEKIDRLHETNKFKLELMPCFPYKP"
     gene            complement(8755..9465)
                     /locus_tag="PLM92_00315"
     CDS             complement(8755..9465)
                     /locus_tag="PLM92_00315"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005999924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HPR23620.1"
                     /translation="MRLNKYIARAGACSRRKADELTINGNVRINGVVMKEPGYDVVEG
                     DEVEVNGKLLSFSEKKIYLLLNKPKGYITTTKDEKGRATVMELVKDVPERIFPVGRLD
                     HDTSGLLIMTNDGELAHYLMHPGNEVTKRYRVFAAGTVSGQKLSRLRKGVEIGGFVTS
                     PARVDIIKQSPRSALVEIEIHEGKNRQIRKMFSAVGCPVQALERIAIGEIRLGRLKEG
                     HYRKMTKSEIEYLKKQMR"
     gene            9625..10398
                     /locus_tag="PLM92_00320"
     CDS             9625..10398
                     /locus_tag="PLM92_00320"
                     /inference="COORDINATES: protein motif:HMM:NF018448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HPR23621.1"
                     /translation="MQKRALILAVRAGELMMKNGAEVYRVEDTITRICRACHIPYVEV
                     FATTTGVFVTIGSGGEGGDVYTYIKSLDGRTTDLKKISDLNKFSREFTDTDLSVESGI
                     KRLSEIDNAKPYPLPIRLLGAGLVASFFCGIFSGHIIDSLCSFIIGVAGYSLASFLDR
                     YDINYFIRGFTCCGLTTFLALLADSSGACSNYGAIVIGTIMIFVPGVAITNSIRDFLS
                     GDMLAGVARMAEALIIAVSLATGAGIIIAAWNMIGGVAL"
     gene            10395..10850
                     /locus_tag="PLM92_00325"
     CDS             10395..10850
                     /locus_tag="PLM92_00325"
                     /inference="COORDINATES: protein motif:HMM:NF018448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HPR23622.1"
                     /translation="MITWEMIVPLIMGFAPTLGFCLLFHVPANHILPAALIGGLGWAA
                     YELWTSTGNNKVIGCFLAACLVGLLSDISARTLKEASTVFSIPGIMPLVPGAGMYYTM
                     LELIKNDFSQAAALGSETLFMAGAIALGLMVVGSVIRIIISIKRKIYST"
     gene            complement(10935..11528)
                     /gene="prxU"
                     /locus_tag="PLM92_00330"
     CDS             complement(10935..11528)
                     /gene="prxU"
                     /locus_tag="PLM92_00330"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014808585.1"
                     /note="Most members of this family contain a
                     selenocysteine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(11391..11393),aa:Sec)
                     /transl_table=11
                     /product="thioredoxin-dependent peroxiredoxin"
                     /protein_id="HPR23623.1"
                     /translation="MLVTKQAPNFSGTAFHEGKIKKVSLEDYRGKWVVLCFYPGDFTF
                     VUPTEISHIAVGYDELKEMNVEVLSVSTDSVYSHKIWNETELSKMVGNDIPFPMVSDQ
                     GGAIGKLYEVYDENGGVNIRGRFLIDPEGVIQAQEVLSPPVGRNPAELGRQIKAYQHH
                     QKTGEVMPSGWNPGGQTLKPSPEMAGNVWKVWTPPKE"
     gene            complement(11613..12203)
                     /gene="scpB"
                     /locus_tag="PLM92_00335"
     CDS             complement(11613..12203)
                     /gene="scpB"
                     /locus_tag="PLM92_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005999549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HPR23624.1"
                     /translation="MTSKRSIKSAFESMMFTWGEPLDMKLAAEIFNIDAKEAYGYFKE
                     LQQEYDDQKRGIRIRETNKKFQFVTESDNFEYIERLCTPAKQRKLSQSALEVLAIIAY
                     KQPVTRGEIEAIRGIRCDRVLEGLQKKELIDEVGRSSGIGRPILYGTTDEFLKNFGFS
                     TLKELPDIEDIESAIEVEEPKDIVDLQQIPIKIPEE"
     gene            complement(12181..12966)
                     /locus_tag="PLM92_00340"
     CDS             complement(12181..12966)
                     /locus_tag="PLM92_00340"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HPR23625.1"
                     /translation="MSYKVKINIFEGPFDLLVYLIENARMSIYDIKVSEITAQYIKYI
                     EEMQKMDVLLASEFMVLAATLIDIKSKMLLPKQVEEEEGGIGEDPRTELVQKLIEYKK
                     FKNASEILARKEEETSRILDKPREDLSFFTNEPDEYLKLNINQFVKAFNDFLIKKKKV
                     DEIRRNYERDERQRITTEVRMDFIRELILKWPEKVFTFKELVTDPQSKYDTALSFVSM
                     LEMIKQRRIEASQKTLFGEIKINATAYLDNGKEEEPDDKQKIN"
     gene            complement(13034..13906)
                     /locus_tag="PLM92_00345"
     CDS             complement(13034..13906)
                     /locus_tag="PLM92_00345"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPR23626.1"
                     /translation="MNLFQRNAKLIVVLAMISGSTSGILGKLITAGSMTIGFYRLSFC
                     LPFFIVPLLIKHRKDIIDINKKQLVLCALAGVFLFGHFFCWYVAVKNTVIASAIVLAG
                     LHPLIVMLFMFVFFRKRTPAKAMAGLLIALSGGILVAGMDYSFAGSNVFGNFMAFGAA
                     VCMGIYFCIGNIVREKMNAGVYIFFVFFFCWVCFAVGMLLTKTPLTGYPLSDYIWLIV
                     MTLLCQFGAHAVLNWSLGYVSSLYVSAIETGEIVSAMILAFIVLSQVPTPWQMAGATV
                     VIAGLLYYNRNDLK"
     gene            complement(13903..14877)
                     /locus_tag="PLM92_00350"
     CDS             complement(13903..14877)
                     /locus_tag="PLM92_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPR23627.1"
                     /translation="MNNTKIYTAMVFTALFWSGAFIAGKLCVPYIPTYTLTFLRFLFA
                     AAVMAVVLMIQNKKAEGKIREQKEGGSPAKPYRLQKKHVPIFLFTGIVGMVGYHVFFF
                     ASLKYTTAINSSIIAAMNPSLTMILAALLLSIKVPVRQAAGVILSFVGVLLTITGGDV
                     GVLMTLSFNKGDILMFIAIICFAFYSVFSRAKAGDIPSTALTFYSFLVCTVFLIPFVI
                     WEKPWTFFGDIPVSAWIGVMYMSIFASVLGYLIQQVSFKRIGASRTNIFINVVPVFSI
                     ILAVTILGEKLEIIKIFTAAMIICGVIMCQLPQSREKIQKKPIEGEDG"
     gene            complement(14870..15940)
                     /gene="mtnA"
                     /locus_tag="PLM92_00355"
     CDS             complement(14870..15940)
                     /gene="mtnA"
                     /locus_tag="PLM92_00355"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005999545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HPR23628.1"
                     /translation="MLDTVPLKFEEGQLLILDQRLLPGEERFIEISTKEETWDAIKKL
                     KVRGAPAIGIVAAYGLYVTLRDIKTKDYEEFFVKFRETKKYLETSRPTAVNLFWALDR
                     MEKKLKEHEGRKDNDIPWEEESKQIKEVLLLEAEKIRREDEEACYSMGSYGLELLRPG
                     MGILTHCNAGALATAKYGTCLAPIYLGKEKGYGFKVFADETRPLLQGSRLTAWELKKA
                     GVDVTLICDNMASAVMKNGWIDAVVTGCDRMAANGDGANKIGTSALAILAKEYNIPFY
                     MYAPLSTIDLNTLSGEQIKIELRNGEEIYKMWYEKDMAPEGTKTYNPSFDVTDHKYIS
                     AVITEKGIVRPPYREGLKKLFE"
     gene            complement(16163..17515)
                     /locus_tag="PLM92_00360"
     CDS             complement(16163..17515)
                     /locus_tag="PLM92_00360"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003414531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HPR23629.1"
                     /translation="MNKAKALEEARRVVGYIESENLSEADKTAIHDDSIKNFNENVNP
                     GWLEYRKSVSDDAAFIEWTDSEEHFEDLHGTQFIDCLGGFGIYTCGHRNPAILKTVKA
                     QLDKYALHSQELVDPLRGYLANILAMITPGDLQYCFFTNGGAEAVEMALKLARLATGG
                     RWYISTVGAFHGKSMGAISMGGKGAYREDYVPMIQQVQHVEFGNADAMEAAIKNLQTV
                     GEKVAGVIIEPIQGEAGVQIPPEGYFKKVREICDRYDVALICDEIQTGMGRCGTMWRV
                     EHEGIVPDIMTYGKAFGGGVMPITGIIARPKMWVEKLIDNPWILGSPTFGGNPLACSA
                     AISTIKFMLENDVPKMCAEKGAYIMAGLEKLKQKYPTVLVGYRGAGLLICMEFPEAEV
                     GYKVTKGMFERHVMTAGTLVNAKTVRIEPPAVLKQETMDEVLAKLDEALADAKKEFNL
                     "
     gene            complement(17851..18327)
                     /locus_tag="PLM92_00365"
     CDS             complement(17851..18327)
                     /locus_tag="PLM92_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006574037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HPR23630.1"
                     /translation="MFEWITATFAGKILATAVVSAVPVIELRGGIPFGVYHGLPIWLS
                     YAVSVFGNMLPIPFILWFLKRIFNWMKSFGKLGIIVQKLEQKGHIKGQKVIKYRKLGL
                     FILVAIPLPGTGAWTGALVATVLEMPWKEALPLIFLGVLAAGFLVTGITFGFTSIF"
     gene            complement(18378..19568)
                     /locus_tag="PLM92_00370"
     CDS             complement(18378..19568)
                     /locus_tag="PLM92_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003493023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HPR23631.1"
                     /translation="MERRVGTVARGLRAPIIREGDDLKKIVTETVLEAAASGEFTVND
                     KDIIGITEAVLARAQGNYATKEQLAKDVRNKLGGEEIGVVFPILSRNRFAICLKGIAK
                     GAKKIFLMLSYPSDEVGNHLVDIDLMYEKGVDPYENVLTQNEFEKLFGKSKHPFTGID
                     YISYYKQLIEEEGAEAEIIFSNDPKTILLYTKNILTCDIHTRERTKQILRDAGAEKVL
                     GLDDILDKSVEGSGSNNRYGILGSNKATEETIKLFPNECDKFVDSVRDDIKKATGKNV
                     EVLVYGDGAFKDPVGKIWELADPVVAPGYTAGLAGTPSEIKLKYLADNDFAHLKGEEL
                     KKAISDYIKNKDADLEGKMVSQGTTPRRLTDLIGSLCDLISGSGDKGTPIVYIQGYFD
                     NYTK"
BASE COUNT         4872 a         4687 c         3531 g         6640 t
ORIGIN      
        1 aaccgccagg agtacggaga tgatcaaatt cattgtctta ttctgaaaca tcttttttcc
       61 tcccaagcct gtttattgcc gactttagcc ctgaaacctg tttttcggtt cccttgttga
      121 tataaacatt aagcagtatc ttttcaactg ttttgatatc aagaaatctg tcaagtttac
      181 cgtcttccgc aatggaaatt atgcctgttt cttcagaaac aacaagaacc agagcatcag
      241 aattctcagt tattcctatg cctgctctgt gccgggtccc cagatccttt gaaagatttt
      301 tgttttgtgt aagaggaagt acgcatcccg cagaataaag cttatcgccc ctgacaatta
      361 ccgcaccgtc atgtaaaggt gaaccttcgt aaaaaagatt gacaataagc tcagccgaaa
      421 tatcggcatt gattatggtg ccggtctcac aaatgtcttc cagggctgtt tctctctcaa
      481 ttacaattag ggcccccgtc ttgtctgtgg acattgtctc cagcgcctcg gcaaactccg
      541 taactatcat tttgcccttg accttgtcca ccttgccgaa caccggtttt attatcttgc
      601 ttcttcccat gtattccagc ccgcgcctga gttcaggctg aaatacaacg ataagggcga
      661 ttatacccac cgtcatcgtt ccctttagta tccagttcag agtatgaagg tggaaccagt
      721 cggatagaaa aaaagccgcc accaaaacca gcagaccttt gacaagctgc tcagccctgc
      781 tttctcttat gaagcccagt atcttatata caacgaaagc cactataagt atgtcgatga
      841 tatccgtgac tcctatgtcg gatgccagat tatttaaaaa tccttccata aagttcttct
      901 taagtcagcc ccctttatca gtatgttgtc tccaaaaggc cgtagtcatc gtcctttctc
      961 ttatatacca cgtttacgct gtctgtttcc atattgagaa aaacaaaaaa attatgttca
     1021 agcatttcca tctggagaat ggcctcgtct gtggtcattg gttcaagttc gaatttcttt
     1081 gtttttacta cttttatttc ttcctgaact tcttcaggcc agtcggcgaa aacaaaatcc
     1141 ttgttgtctt tatgtttttt ctgtatcttg gtcttgaatc tggacatttg agtcgatagt
     1201 ttttcgacca cgttatctac cccttcatac atgtcaccgg atacatcttc cgccctgaaa
     1261 atcatgcctc ttacccttat tgtcgcctct attttctgtc tgttcttctc tgcggaaagg
     1321 gtcacattca caattatatt atcggaaaaa tatttgctca atttctccat tttcttttcg
     1381 atagtatctt ttaaacgatc gtttgcattc acatttttgc ttgttattac aaccttcatt
     1441 tcatgtacct cctttgctga atatatgcat aaaacaaccc agttttatta taacatatct
     1501 ttttctcata atacaatagt cgcttcagct gacctggtct ttgcccccac acctgcctgc
     1561 cggactttgt ccggaggact tacatctaga ctgcgccatg ctcgccgcaa aaagcggttt
     1621 acgtgggtcc ttttgcccgc agctggcctg gactttcaac cacagacctg aagcgactat
     1681 tctatcccct tgataaattc cctcccgctg caaatgtcag aatatccact gccgatgccc
     1741 ccttttttag aagctcaagg ctgcagctgt caagagtact gccggtggtg aatatatcat
     1801 ctacaaggag tatttctttt tctattatat ccttttctct gccttttttg atgcagaatg
     1861 catttttgac gttgctcctc ctttctgccg tatccagagc tttcattggt tctgtatttt
     1921 tgtttcttat cagtatgctg ccgtcaaatg gaatatctgt tttttctgca aagtattttg
     1981 ccagaagttc cgactgatta tatcctcttt tcttttccct tgaaggatgt accggcacag
     2041 gtatgaccat atcccatatg atttcttccc ttttcatcct gtctgccatt atttctgcca
     2101 gttttcttcc tatatatgac ttgtcgttat acttgtaatc catcattact gctctttcat
     2161 agagtccgta acgggaacat gtataacctt ttctgaaaac atgttccgta tccatgcagt
     2221 catgacaata atcattttta tatgtttctt ccaggatctt accacatttt ttgcatgttt
     2281 tgctgatggc ccagtgaaat tttttgatac aggaatcaca caaagcataa ggcctgcttt
     2341 tgtctataat acttccgcag catatacagt atatattaga aggaaatata acttcgaata
     2401 tattttcttt tattttcata ctttcgcctt tttctctttt tatatgcttc ctctgtccag
     2461 gaatccttga agtcttgcct tgagtccgga atatcgctct ttgatgctgt tattatccac
     2521 cattgcctgc attcttattt cagatcctac tactataaca gctttttttg ctcttgttac
     2581 agcggtataa agaagatttc tggtagaaag cacaggcggg aaccatgata cgggaatcac
     2641 gaccacaggg aattcactgc cctggctttt atgtacagtg acggcataag caggctccag
     2701 ttcatccaac tgggaaaaat cataaacaac atacttgtta ctttcatatt ctactgtaag
     2761 ttcattgaat tcattgtcta ttttttttat tgttcccaca tcgccgttaa aaactccttc
     2821 tccttcggca aaatcatccg ccatcttcca tttaagcata taattgtttt ttatctgcat
     2881 cactttgtca ttttcacgaa atattttttc tccgtatact ttttctgaaa gtaattcgtt
     2941 ggagggattc attatgctct ggatctcttt attgagattg acgcagccca gaagcccttt
     3001 cctcacaggc gtcaatacct gtatgtctct cgatggatcc agatccttat agtatgaggg
     3061 cagtctgttt ccgcaaagtt ccaatataga agctaatact tctctttcac ttttttttct
     3121 gatcataaaa aaatctttgt cttttgtatt gcaatcggga tattctcctc tgtttatcct
     3181 gtgtgcgttt acaactataa ggctttctgc cgcctgtctg aatatctcgt ttaattttac
     3241 tgaatatata aattcacttt ccagtatatc tccaagcaca tttccggctc ccacagaagg
     3301 cagttgatcc gaatctccca ccattatcag tctcgtaccg ggttttacgg catttagaag
     3361 tccgttcata agcatcagat ctatcatgga gctctcatct attataactg catcctgttc
     3421 aaggggattt tccgcatttc ttccgaaaaa catagcatct tcggattccg aataatagta
     3481 ctcaagaagt ctgtggatag tagaggcttc atgtcccgtt gtctcggtta ttctttttgc
     3541 cgctcttccg gtgggagccg ccacagctac actcatgtcg cttttgatca gtatctttat
     3601 tataccgttt attattgtag ttttccccgt acccggacct ccggttataa cgcaaacacc
     3661 attactgata gaagtctcga tggcatattt ctgattttcc gaaaagtgta tgccggtatc
     3721 tgtttcagca gcggcaataa ggcttcctat ttgcgtgctg acgggtttaa ggtctgcttt
     3781 ctcgaggttt aacagccttt tgcatacgtt ttgttctgcc ataaaatagg gcataagaaa
     3841 taccgcattc ctgtcttcga ttttttctat tcttatttca tcttcaaaag ccattgccgc
     3901 aagaacatca ttgatgagaa aactttcaat atccatcagc tcccctgctt tctcacaaag
     3961 ctccttttgt ggcacaaaag tgctgcccga tgaggcgaaa tgcataagaa caaattttat
     4021 tccgcttttt atcctgaatt catcatcttt gtccactccc attttttccg caataaggtc
     4081 agcctttttg aagcctatcc cgaaaacatc ttctgtaagt ctgtaggggt tttccttaac
     4141 tgcttctatc gtatctttgc catataccct gtaaagtttc atggcatact ctgcacttat
     4201 tccaaactgc tggaaataca aagtcatttc agcaaattct ttatgtgtct tgaaagcctc
     4261 tgctatggat cccgcccttt ttttgcctat acccggcact tctgtaagtc tttcagcctc
     4321 ttcttctatt attctgaaag tgtcctctcc gaattttgat gctatggcct ttgccgcctt
     4381 tggacctatc cccttcaaaa tgccggaagc cagaaaaccc tctatgccct ctgcagagga
     4441 aggcagcacc tcacggctct gagtgaatac aaactgctct ccgtattttg gatgtatctt
     4501 gaaatctccc cgcaaaatga aagtgccgcc ttttttgcaa cccggcagta tccccaaggc
     4561 tgtgaagctt tcttcttccg actccacata tgcaatagta taggcatttt ccttgttatg
     4621 aaaaattatt tcttttattt ctgctttttt ttcagtcatt cctgctccat gataaaatcg
     4681 ctttaccctt tttttcttcg tgcgccccga ataagaaaag atggcgtcca cttatgtgac
     4741 gccatctctc tgatcaaaca agaattattt gataatatct ttatctttgt tttcttttcc
     4801 gtcatctgtt actgtccccg agattttatt ttcatcattt ttttccgtca cattgctatt
     4861 gaaagcagca ctgttttcca ttgtcggggc tctcaggtcg cggggagggc ttacttcaaa
     4921 atcaaattca tcttctttta catttatctc cttgcttata atactgcctt tgacccttgc
     4981 acccatgtct attgccactt ttttcgtatc tatgtctcca ataagtatgg ctcccgaatc
     5041 aagttcaact ttctcttttg catataaagt gcctttaagt ctgctgtcca gtatgatatt
     5101 gctggcatga acatctccga gaatagtaca ttctttcatt tccgcatcat tttcagcatc
     5161 tatccttcct ttgatagagg ccttgttgag caatatattt ttgcctttta tgtccccctc
     5221 tatcttgccg gaaacctcaa gatcttcagt cgtattgaca tttcccttta cttccccttg
     5281 tatctcaacg atgccagtag aaaaaatgtc cccgattatt gttgtaccgt tgccgataac
     5341 tattggttgt gccttaacat tggcatttat gcccgtatct gcggttttgc caaaataatc
     5401 cttttttcca ttttaatccc tccgttgtag taatttctct tcgaataaca actcctttgc
     5461 gaatacactt aatcgacatt ttgttaatca actgtgttat tatatcattt tttcctttca
     5521 atgcaaatat tatatttctt ttatcccggt aagataaggg gttatctaag ccactttacc
     5581 ttcctcgttc ttgctgccag tttgtttttg ttgaactttc ttgcatattt cagtaatgtt
     5641 ttcttattgt tgtcctttgg ttttttatga acgacatccg tcagcatcag taaaaggtgc
     5701 cccacctgtt ctccttctgc tataccggct tttattatat cgtcgccgtt tatggcaaga
     5761 tcctctatat atatgggctc tttgttctcc tttattattt tgagaagttc atgtctggaa
     5821 attatttttg catcccccag gtcatagacg attctctggg cctttgaaag attgtccaga
     5881 tattcatatc ttttcggtcc gtatttaaca aggaaagctt tcagatctac tcctgttctc
     5941 aagaaatgaa gcttctccat cataagaaga gcgtccttca gggattcttt tgtgttttcg
     6001 tcataatcca gcatctcaat ggcctgcata cccttttttt cttcaaaaca ggtataaaag
     6061 agtccaaggc gccttttaag cacaggtttt gtcttatcta tgttgtcaca gagggtcgag
     6121 aactgctgca tttgtcttct gttgagcttt gcagccacat cgcctattat ggccggcata
     6181 aggtctgccc ctgccagcat tctcagccct ttgcctgcgt ttggacaagt tatgatcctc
     6241 tcaaactctt ctctgatttt ttttatattt gcttttgata acagtcctga gtttgccacc
     6301 atagcttcat acgtcttttt gtatatatca aaattcagtt ctgctgccac tctcacggct
     6361 cttatcattc taaggggatc ttccgaaaat ctttccatgg gatcgccaac cgttcttata
     6421 agccgctttt ctatgtcttt tcttccttca aaagggtcta cgattttgcc tgacgggtta
     6481 tctgcgatgg cattaacagt aaaatccctg cgtttgagat cttcttctat gcttttgacg
     6541 aattccacac tgtcaggacg ccttttatca gaataaggac cctcttttct gaaagtggcc
     6601 acatctgcta ttatcccatc ctcgtcgtct tttgtatgat ctatccttat gacactgtat
     6661 ttttcactta ttacttttgc cgcaggaaat atttttttca tatcctttaa ttctgcattt
     6721 gtggcaagat cccagtcaat gggttgttgt cctgtaaggg agtctctcac acagcctccc
     6781 acaacatagg cagaaaagcc tttcttttcg agggccttga ttatattatt tacttccttt
     6841 gggagcttta tcacttttct gtttcctcct tttcgatacg tctgtatatc cttctgtttt
     6901 ccatagtcca aaccctttca ctgaattcac cgggcatggt tttttcgatg gccttttcca
     6961 tgtcgtacat cctggcatct atcatatcca gataatgaag cagctccgct tcggggaaaa
     7021 ggggtttttt cggacttccg tattccggct catagtggtg tgagagtacc atatgttcca
     7081 ccattattct tttttccgat gagaaatcca tttctaacat ctctttatcc agcactttta
     7141 tgctctggat tatatgtccc agcatcatgc cttcaaatga atatccgtct gatatgccgt
     7201 actcgtttgc ctcgatctcc cttatttttt cggtgtcatg aagtatgact cctgcagcca
     7261 ccagatcttt gtcaagccca ctatagacct gacatacttt ttctgccgtc ataagcattc
     7321 gtttcatatg ccaaagcaac cccgccattt cagcatgatg atttttctgg gccgccggat
     7381 aatacatgag tttttctttg ttatctttaa gaacttttaa acaaagcttc ctgaggtcac
     7441 tgtcgctgat ctctccggct ttgtcaaata tataatcata catttcttca ggcttttccg
     7501 gagctgcttt tatataatct tttatatcct gcagatcttc cttctgtcct ttgcgtattc
     7561 tggttatctt gagttgcttc tgcccgttcc attcagtaac ctgggccttt attttaacaa
     7621 ggtcccctgc ctctatacgg gaaagtcctt ccagttcttc atcggccaga tcccattttt
     7681 tgcccgttat ctcccctgtc atatcagaaa gcaaaacatc cagatactgt ttcttgtttg
     7741 atcccatctt gacagcaatc atttttacca taaaaaaatc acttatttcc ttttcgtctc
     7801 ctatggtggc tatataaata tctttcattg atttttctcc tttttatagg acaattatat
     7861 actattgact gtcttattca aagaacaaaa aacgttatca atgtttattt tcgccttcac
     7921 tgtgacagtt tttgttgtat actgttttat atatgtatca tcttgagaaa ggatttgttt
     7981 ttatgttttt cgatccaaaa aaaaccaaag atgaaatagt tgtatggatc cgggaattct
     8041 ttcagaaaac cggaccctct tgcaatgcga tagtaggcgt ttcggggggc aaagacagct
     8101 ccgtggttgc tgctctttgc agtgaagccc tgggcagagc aagggtcata ggcatactga
     8161 tgccaaaggg ccatcaggaa gatatcaaca tggctcataa gctcatatcc atccttggaa
     8221 taaaatcata tacagtaaac atagaagact gttacaaagg tctaatgtgc caccttgaaa
     8281 atgaacttcc tgaaataaca gaaagcaccc gtataaatct gcagccccgg cttcgtatgg
     8341 ccacccttta tgctgcctcc cagtctctta acggcagagt tgccaatacc tgtaatcttt
     8401 cagaggactg ggtgggatat tccacaagat acggagattc tgtcggggat ttcagccctc
     8461 tggcaaacat cacagtagga gaagtcctgg ccctcggtaa ggaactgaaa cttcctgatg
     8521 atatagtgaa taaagtgccg gtggacggtc tttgcggtca aaccgatgaa gagaaactcg
     8581 ggttcagtta caaaatgctt gatgaatata taagaacggg agtatgcgag gataccgcat
     8641 tgaaagaaaa aatagacagg ctgcatgaaa ccaataaatt caaactggaa cttatgccct
     8701 gctttcctta taagccctga tatgggaata tgatgcttct attttctcgg ttttttatct
     8761 catctgcttc ttcaggtatt cgatctcgct ttttgtcatc tttctgtagt gaccttcctt
     8821 taagcgcccc agcctgattt ctcctatggc aattctttcc agcgcctgga caggacagcc
     8881 gactgcagaa aacatttttc gtatctgacg gttttttcct tcatgtatct ctatctccac
     8941 cagggctgat ctcgggctct gttttatgat atccacccgt gcgggagatg tcacgaatcc
     9001 tcctatttcc actccttttc tgaggcgtga aagtttttgc cctgaaactg ttcctgcagc
     9061 aaaaactctg tatcttttag tcacttcatt ccccggatgc ataagatagt gggccagttc
     9121 tccgtcattg gtcattataa gaagacccga tgtatcatga tccagcctgc ccacaggaaa
     9181 tatcctttcc ggtacatctt tgaccagttc catgacggtg gctcttcctt tttcatcctt
     9241 ggtagtcgtt atgtatccct tgggtttatt gagcagcaga tatatttttt tctcggaaaa
     9301 agacagcagt ttgccattta cttcaacctc atcaccttcc actacatcat aacccggttc
     9361 tttcatgaca accccgttga tccttacatt tccgtttatt gtaagctcat ccgcctttcg
     9421 cctgctgcag gcacccgccc ttgctatata cttgttaagt ctcatttatt tactccttca
     9481 aaaggatacc atccctcttg ttctttacac acttgatgtt ccagtcaatt ttagcacatg
     9541 ccatataaaa ttgaaagcac gctgaaaata tgatatccta atgtcatgga tgatcaaaac
     9601 aaaaagcccc gtacaatttc aaatatgcag aagcgggcac ttatcctggc agtaagagcc
     9661 ggtgagctca tgatgaaaaa cggtgcggaa gtttacaggg tagaagatac cattacccgg
     9721 atctgcagag cctgtcatat cccatacgta gaagtttttg caactaccac gggtgttttt
     9781 gttaccatag gctccggcgg agaaggcggt gatgtttaca cctatataaa aagtctggac
     9841 ggacggacga ccgatctcaa aaaaatttcg gatctcaaca agttttccag ggaattcaca
     9901 gatacggatc tttctgtgga atccgggata aaacgcctta gtgaaataga caatgcaaaa
     9961 ccttatccgt tacccatcag gcttttgggg gcaggccttg ttgcctcttt tttctgcggt
    10021 attttttccg ggcatataat cgattccctc tgctcattta ttataggtgt cgccggctat
    10081 tctctggcat catttcttga ccgttatgac ataaactatt ttataagagg ttttacatgc
    10141 tgcggtctta cgactttttt ggctctttta gccgactcca gcggggcatg cagtaattac
    10201 ggtgcaatag taataggcac cataatgatt tttgtccccg gagttgccat caccaattcc
    10261 atcagagact ttctctccgg agatatgctg gcaggtgtgg caagaatggc agaagcactt
    10321 ataattgccg tgtctctggc cacaggagca ggtataatca ttgccgcttg gaacatgatc
    10381 ggaggtgtcg cattatgata acatgggaaa tgatagtgcc tcttataatg ggttttgctc
    10441 ccactctcgg attctgtctc ctttttcatg tgccggccaa tcatatcctt cccgccgccc
    10501 tgatcggagg gctgggctgg gctgcttatg aattgtggac atccacaggc aacaataaag
    10561 tgataggctg ttttctcgcc gcctgcctag tgggtcttct cagcgacatc agtgcccgta
    10621 cactcaaaga agcctccaca gttttttcca taccgggcat aatgcctctt gtccccggag
    10681 caggcatgta ttacaccatg ctggaactga tcaaaaacga tttttcacag gccgccgccc
    10741 tggggtccga gacccttttt atggccggtg ccattgcatt gggccttatg gtggtcggtt
    10801 ctgttatacg aataataatc tcaataaaaa gaaaaatata ttcaacataa attataagac
    10861 ctgcccaaat atatgagcag gtctttattt ttttgttctg taattataaa aatatggatc
    10921 tcatatgatt ttttttattc ttttgggggt gtccatactt tccatacgtt ccctgccatt
    10981 tcaggcgaag gcttgagcgt ctggccgccc gggttccatc ccgaaggcat tacctctcca
    11041 gtcttctgat gatgctgata cgcttttatc tgtctgccca gttcagccgg atttcttccg
    11101 acgggagggc tcaaaacttc ctgagcctga ataactcctt ccggatctat aaggaatctt
    11161 ccccttatat tcactccgcc gttttcatca tatacttcgt ataatttgcc aatagctccg
    11221 ccctgatcgg aaaccatggg gaaaggtata tcgtttccaa ccatcttgct taattcggtt
    11281 tcattccaga tcttatgaga ataaacactg tccgtgctta cagatagcac ctccacattc
    11341 atttctttta attcgtcata acccacggct atgtgtgata tctcagtcgg tcaaacaaat
    11401 gtgaaatctc ccggataaaa gcaaagaacg acccatttgc ccctgtaatc ttctaaggac
    11461 acttttttga tttttccttc gtgaaaagcg gtcccggaaa aattcggtgc ctgtttggta
    11521 actaacatgt catcactcct ttctaaacaa caaaggtctt ttttcatact aacatgctgt
    11581 ttgaaggaat gtcaatcggc taagttgaag gatcattctt ccggtatttt gatcggtatc
    11641 tgctgcagat caacaatgtc ctttggctct tcaacttcta ttgcactttc tatgtcctcg
    11701 atatccggta gttctttcag tgttgaaaaa ccgaaattct tcagaaattc atccgttgtg
    11761 ccgtaaagga tcggtcttcc tattcccgaa gatcttccta cttcgtctat cagttctttt
    11821 ttttgaagtc cttcaaggac tctgtcacat cttattcccc ttatcgcctc tatttcccct
    11881 cttgtgacag gctgcttata ggctattatt gccagtactt ccaaggcaga ctggctaagc
    11941 tttctctgtt ttgccggagt acaaagcctt tcgatatact cgaaattatc gctttcggtc
    12001 acaaactgga attttttgtt tgtttctctt atccttatgc ctcttttttg atcatcgtat
    12061 tcctgctgca gttctttgaa gtatccgtaa gcttcttttg cgtctatatt gaatatttca
    12121 gcagccagtt tcatatcaag gggctccccc caggtaaaca tcattgattc gaatgctgat
    12181 ttaattgatc ttttgcttgt catccggctc ctcctctttc ccgttatcaa gatatgccgt
    12241 cgcatttatt tttatttctc cgaaaagtgt tttttgactt gcctctatcc tgcgctgttt
    12301 tatcatttca agcatcgaga caaaagaaag agctgtatca tatttgcttt gcgggtccgt
    12361 caccagttct ttgaatgtaa aaactttttc cggccatttc aggataagtt ccctgatgaa
    12421 atccatcctt acttctgtgg ttatcctctg cctctcatct ctttcgtaat ttcttcttat
    12481 ttcgtccact tttttctttt ttatgaggaa atcattgaat gcttttacaa actgattgat
    12541 attcagctta aggtattcat cgggttcatt tgtgaaaaat gacagatctt cccggggttt
    12601 atccagtatc cttgaagtct cctcttcttt ccgggcaagt atttcagatg catttttgaa
    12661 tttcttgtac tctataagct tttgtacaag ctcggtcctg ggatcctcgc cgatgccgcc
    12721 ttcctcttcc tctacctgct tggggagaag catttttgat tttatatcta ttaacgtagc
    12781 cgccagcacc ataaactcgg atgccagcag gacatccatt ttctgcattt cttcaatgta
    12841 tttgatgtac tgggctgtta tttctgacac ctttatatca tatatgctca ttctggcatt
    12901 ttcaataaga tatacaagga gatcaaaagg tccctcgaag atatttattt tgactttata
    12961 gctcatttct gctcccttta gtctttatat gcacgacata aattataata caaaactcct
    13021 gtcaaaacca tatttattta aggtcattcc ggttatagta aagaagacct gctattacca
    13081 ctgtggcacc tgccatctgc catggtgtgg ggacctgcga aagtactata aaagccaaaa
    13141 tcatcgcaga aacgatctcg ccggtttcta ttgcggagac ataaagagat gagacatatc
    13201 ccaggctcca gttgagtacc gcatgggcgc caaactgaca aagcaaagtc attacgataa
    13261 gccatatata gtccgataat ggatatcctg tcaagggagt ttttgtcaat agcattccca
    13321 cggcgaagca tacccagcag aagaaaaaaa caaaaaatat ataaactccc gcattcattt
    13381 tttctctgac tatattgcct atacaaaagt aaatgcccat gcatacagcc gccccgaaag
    13441 ccataaaatt accgaacaca ttactgccgg caaaactgta atccattccg gccacaagga
    13501 tgcctcccga caatgcaatc agcaatcctg ccatggcctt tgccggtgtt cgttttctga
    13561 aaaaaacgaa cataaaaagc attactataa gcggatgcag gcctgccagt actattgcac
    13621 tggctattac ggtatttttt accgccacat accagcagaa aaaatgtccg aataaaaaca
    13681 ccccggccaa tgcacagagt accaactgtt ttttgtttat gtcaataatg tctttgcggt
    13741 gttttatcaa taaaggaaca atgaaaaaag gcaaacagaa agaaagtctg taaaatccta
    13801 tagtcattga tccggcagtg atcagttttc ccagtattcc ggaggtcgat ccggatatca
    13861 ttgctaaaac aacaataagt tttgcattac gctgaaacag attcatccgt cttcaccttc
    13921 tatcggtttt ttttggattt tttctctgct ctgcggcagc tgacacatga ttaccccgca
    13981 tataatcatt gccgcagtaa atatttttat tatttccagt ttttctccca atatggtgac
    14041 cgccagtatg atggaaaaca ccggtaccac atttataaag atatttgttc ttgatgctcc
    14101 tatccttttg aatgatacct gttgtatgag ataacccagt acagaagcaa atatactcat
    14161 atacataact ccgatccatg cggaaacagg tatgtcccca aaaaaagtcc agggcttctc
    14221 ccatatcaca aagggtataa gaaaaaccgt gcaaacaagg aaactgtaaa aagtaagagc
    14281 tgtagaaggg atatctcccg cttttgccct gctgaaaaca ctgtaaaaag caaaacatat
    14341 gatagctata aacatcagta tgtctccctt gttaaaggat aacgtcatca ggacaccaac
    14401 atccccgccc gttattgtaa gcagtacccc gacaaaggaa agtatgaccc ccgccgcctg
    14461 tcttacgggc actttgatcg aaagaagcag agccgccagg atcatagtca gtgaagggtt
    14521 cattgcggcg attatggatg agtttatagc cgtggtatat ttcaggcttg cgaaaaaaaa
    14581 cacatgatat ccgaccatgc ctactattcc ggtgaaaaga aagatgggca catgtttttt
    14641 ctgcaaccgg taaggtttcg ccggggatcc tccttctttc tgttctcgga tttttccctc
    14701 cgcctttttg ttttgtatca taagtacaac ggccataact gccgctgcaa aaagaaatct
    14761 taagaatgta agcgtatatg ttggtatata cggcacacac agttttccgg caataaatgc
    14821 accggaccag aaaagagccg taaagaccat ggctgtgtat atttttgtgt tattcaaaga
    14881 gttttttcaa tccttccctg taaggcggcc ttacgatacc tttttccgtt attaccgcac
    14941 ttatgtattt atgatccgta acgtcaaaag aaggattata tgtctttgtt ccttcaggtg
    15001 ccatatcttt ttcgtaccac attttatata tttcttctcc gtttcgaagt tctattttta
    15061 tctgctctcc ggaaagagtg ttaagatcta tcgtggataa aggggcatac atataaaagg
    15121 ggatattata ctcctttgcc agtatcgcca gggcagaggt gcctatcttg tttgctccgt
    15181 caccatttgc cgccatacgg tcgcatcctg ttactactgc atctatccat ccgttcttca
    15241 tgaccgcgga tgccatatta tcacatatga gagtcacatc tacaccggcc ttttttagtt
    15301 cccatgcggt caaccgcgat ccctgaagaa gcggtcttgt ttcatctgca aatactttga
    15361 atccatatcc cttctctttt cccaggtata tgggtgccag acatgtgccg tactttgcgg
    15421 tggccaatgc tcccgcattg cagtgggtaa gtatgcccat tcccgggcga agcaattcaa
    15481 gtccgtaact tcccatggaa taacatgcct cttcatcttc tcttcttatt ttttcggcct
    15541 caaggagaag tacttctttt atctgctttg attcctcttc ccatgggata tcattatctt
    15601 ttctgccttc atgttcctta agtttctttt ccattctgtc aagtgcccag aaaagattga
    15661 ctgctgtagg tcttgatgtt tcaagatact tttttgtttc ccggaatttc acaaaaaatt
    15721 cttcgtagtc tttggttttt atatctctca atgtcacata tagcccgtat gcagctacaa
    15781 tgcctatggc aggcgctccc ctgactttca gcttttttat agcatcccaa gtctcttctt
    15841 ttgtgcttat ttctataaat ctttcttctc cgggaagcaa cctctgatcc aggatcagaa
    15901 gttgcccttc ttcaaatttc aatggtactg tatcaagcat ttgttttctc cttttttgag
    15961 acacaaatca attatactat aacagaaagg atattgcatt cagaaaatcc cataaagatc
    16021 aagacggata tttctcttgt tacatgtagc acatttaaaa tatatcatgt ttatcttgcc
    16081 tccgtaacgg tgtttcaaag gacatgaaaa agaccggcaa aagccggtcc tttttgttta
    16141 ttcaatagaa ttttttgttt ttttagaggt tgaattcctt ctttgcgtca gccagagctt
    16201 cgtccagctt agccagaact tcatccatgg tttcctgctt gagtactgct ggcggctcaa
    16261 tacgaactgt ctttgcatta accagagttc ctgcagtcat aacatgcctt tcgaacatgc
    16321 cctttgttac tttgtatcct acttctgctt ccgggaattc catacagatc agaaggcctg
    16381 cgcctctgta tccaaccagt actgtcggat atttctgctt gagtttttcc agtcctgcca
    16441 tgatatatgc acctttttct gcacacatct tgggaacatc gttctccaac atgaacttga
    16501 tagtagaaat agctgctgag caagccagtg ggtttccgcc gaatgtgggt gatccgagta
    16561 tccatggatt gtctataagt ttttctaccc acatcttggg ccttgcgatt atacctgtga
    16621 taggcataac tccgccgccg aatgctttac catatgtcat gatgtcggga actatacctt
    16681 catgctctac tctccacatg gttccgcatc ttcccatacc tgtctggatc tcgtcacaaa
    16741 taagagcaac atcgtatctg tcacatattt ctcttacttt cttgaagtat ccttccggtg
    16801 ggatctgaac gcctgcttca ccctgtattg gctcgatgat aactcctgct accttttcgc
    16861 ctactgtctg cagatttttt atagctgctt ccatggcatc ggcattaccg aattcaacat
    16921 gctgcacctg ctggatcata ggaacataat cttctctgta tgcgcctttg cctcccattg
    16981 agatggcacc catggatttt ccgtggaatg cgccaacagt tgagatgtac catctgccgc
    17041 ctgttgccag tcttgccagt ttaagagcca tttctacagc ttctgctccg ccgtttgtaa
    17101 agaagcaata ctgaagatct ccgggagtta tcattgcaag tatattagca agatatcctc
    17161 tgagcgggtc aaccagttcc tgtgaatgca gtgcatattt gtccagctga gccttaactg
    17221 tcttgaggat agcagggttt ctgtggccgc atgtgtagat gccgaatccg cccaaacagt
    17281 cgatgaactg tgttccgtgc agatcctcaa aatgctcttc cgagtctgtc cattcaatga
    17341 atgccgcatc gtcggataca gattttctgt attccagcca tccggggttt acattttcat
    17401 tgaagttttt gatggagtca tcgtgtatag ctgttttgtc agcttctgac aagttttcac
    17461 tttcaatgta gccaacgact cttcttgctt cttctaaagc ttttgcttta ttcatttcag
    17521 tcattctatg acctcctctt ttctcaccgc ccaaatttta gcggctaaaa atataacttt
    17581 gtacctttat tataatacaa gtttcgtgcc aagatgttaa aaaaatgaca tctttttgag
    17641 acgaaagcct gcttcccact tgtttcaatg aatcctgcat atgacttttt tgaggtgtta
    17701 taaacgccgc taatatgaca gcaaacttgt aacataacaa aagcaatttg tcaaaatctt
    17761 ctctcataaa tcatacaaaa aagttaagcg ctgtctcact tctgagacag cgctttttat
    17821 cagcaaatac ttatttcttt caccaaatct ttaaaaaata cttgtaaatc cgaaagttat
    17881 tccggtaacc agaaatcctg cagccagtac acctaaaaat attagcggaa gagcttcttt
    17941 ccagggcatt tcaagaacag tggccaccaa tgctcctgtc catgcgccgg tccccggaag
    18001 aggtatcgcc acaagtataa acaaccccag ttttctgtat tttatgacct tctgtccctt
    18061 tatatgtcct ttttgctcca gcttttgcac gatgattccc agctttccaa aggatttcat
    18121 ccagttgaat atcctcttca aaaaccatag tataaaaggt atgggaagca tattgccaaa
    18181 aacgcttact gcataagaaa gccatatggg caggccatga tacactccaa aggggatacc
    18241 tcctctaagc tcaataacag ggacagcaga tactactgca gtggcaagta ttttaccggc
    18301 aaaagtggca gttatccatt caaacatact gtcagcttcc cctctctttt ttgtgtggtc
    18361 cgttttttat cataaaatca ctttgtataa ttatcaaagt atccctgtat ataaacaatg
    18421 ggtgttcctt tatctccgct gccggatatt agatcgcaca gagagcctat cagatcggtg
    18481 agtcttcttg gcgtggtccc ctgggaaacc atttttccct caaggtccgc atccttattc
    18541 tttatataat cagaaatggc ctttttgagc tcttctcctt ttaaatgagc aaagtcatta
    18601 tccgccagat atttcagttt tatttcgctg ggagtccctg caagacctgc ggtatatccc
    18661 ggcgccacaa cagggtcagc cagttcccat atcttgccga cagggtcttt aaaggctccg
    18721 tcgccgtaaa ccaggacttc cacatttttt cctgttgcct tttttatatc atctcttaca
    18781 gagtcaacaa acttatcgca ttcattggga aaaagcttta ttgtctcttc cgttgctttg
    18841 tttgatccaa gtatcccgta tctgttgttt gatccactgc cctcaacgct tttatcaagt
    18901 atatcatcaa gacccaggac cttttctgcc ccggcatccc ttaggatctg tttggttctt
    18961 tctcttgtat gtatatcgca ggtcaggata ttttttgtat aaagaagtat tgttttggga
    19021 tcgtttgaaa aaattatttc agcttcggca ccttcttctt cgatcagttg cttgtaataa
    19081 cttatataat caattcctgt aaaaggatgt tttgatttgc cgaacagttt ttcaaactca
    19141 ttttgtgtca gaacgttttc atatggatcc acaccctttt catacataag gtctatatca
    19201 accaggtgat tcccgacttc atcgctggga taacttaaca tcaaaaatat ttttttggct
    19261 ccttttgcta ttcccttaag acatatggca aatctgtttc tgctgagtat gggaaaaaca
    19321 actcctattt cttccccgcc caacttgttt cttacgtcct ttgccagctg ttcttttgtt
    19381 gcataattgc cctgagctct tgcaagaacg gcttcggtta tgcctataat atctttgtca
    19441 tttacagtaa attcaccgga agctgccgcc tcaagcaccg tttcagttac tatttttttg
    19501 agatcgtctc cttctcttat gattggtgcc ctgagtcccc ttgccaccgt tcctactctt
    19561 ctttccatct tcttttctcc tgtctgtttt ttggggaaca gcctgctgtt ccctgtcaat
    19621 tatacaacct gaaaggctta aatacaaatg ttttaagcct tcctgagcct taatgcattg
    19681 gtaactacac aaagagaact catagacatg gctgcagcgg caaacacagg
//
LOCUS       DAONHK010000004        16724 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_2703_length_16724_cov_5.098446, whole genome shotgun sequence.
ACCESSION   DAONHK010000004 DAONHK010000000
VERSION     DAONHK010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 16724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16724
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2703_length_16724_cov_5.098446"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            232..1560
                     /locus_tag="PLM92_00375"
     CDS             232..1560
                     /locus_tag="PLM92_00375"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HPR23632.1"
                     /translation="MKKESAFSDTMAGLVSEFDRSHEEFAQKTEAVVSKGNRRLRFLR
                     VMADRKRVLLLKYFAGIVAVTVGVLTLFNYVTGYEYSYNGKVLGLVKGQKEVTQVLEI
                     ASDQLTDHYNAKITIGEKEDIQFRKVVTINRDVDDMEEVLKRLTYMKDINASGYGIYV
                     NEKRMAALATKKEAKKVLRETLAKFSRKKDGVKYEEVKFAEKVKIKTVDTKLGNIQKI
                     AAVQGKMFADEDSPMLHVTTVEKAVYKEKVHYETKKKDNPDAYIGENSTTVKGKDGEN
                     EVVARITRINGKEIKRDILKTKELTKPVTKVVLVGTKKIPPTMGDGKYCNPCPAGYVS
                     SGFGYRWGRLHRGVDLACSSGNSIYAADGGTVVSAGYRNSYGNVVEINHRNGVVTRYA
                     HCSSILVHAGQKVYEGKLIARVGSTGNSTGPHLHFEMQINGRFVNPLKYI"
     gene            complement(1557..2567)
                     /locus_tag="PLM92_00380"
     CDS             complement(1557..2567)
                     /locus_tag="PLM92_00380"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPR23633.1"
                     /translation="MKFLRNKEIIGQMTVFGILTALFTVAAFFVRWYCSLLVLAVAVS
                     FCLIFLISTKKRYDRIEKLSEKLNLILHGQDFLAIDDNQEGELAILESEIYKMTVKLR
                     EHSYALQKEKIYLTDSIADISHQLRTPLTTINLIMSFLQKKDLSEKKRLEYNQEINQN
                     LIRIDWLISSLLKISKIDAGTAGFKNEHVDVRELIDRSFDPLLIPIEIRQQTFSLEVT
                     GKPGFMGDINWTCEALGNILKNCMEHTPEGGHLKVTAHENALYTEIIITDTGNGIDPD
                     DMPHLFERFYKGKNATDSSTGIGLALAKMIIQKQNGSIKLENRIEGGAKFTIRFYKKP
                     EN"
     gene            complement(2621..3310)
                     /locus_tag="PLM92_00385"
     CDS             complement(2621..3310)
                     /locus_tag="PLM92_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPR23634.1"
                     /translation="MNKILLVEDDISIVENLKALLEEEEFTVEVSTTQEQALKLLENS
                     KFDLLLLDISLPDGSGYAVCSAAKANSVSNLNIETPVIFLTAYDDEATAVTGLDMGAE
                     DYITKPFRPRELVSRIKNILRRSGKSKPVIEIGDLKVDSLKGTVHRKGEEIFLSVLEY
                     RLLLVFLNNKGVILSRNKLLEEIWDIAGEFVNDNTLTVYIKRLREKIEDDPQNPEIIK
                     TVRGMGYKVGE"
     gene            complement(3542..3892)
                     /locus_tag="PLM92_00390"
     CDS             complement(3542..3892)
                     /locus_tag="PLM92_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPR23635.1"
                     /translation="MKRVIVTNNKKVKEAFEGKADVKYFNATAIDIFEEARELAKSGG
                     KMLVDPTRMPVKNYYRSIPFLVDGGAPSERSVDLIESCLAKLEGKKQGFEKEPVLAGL
                     QENKDMETIKKVIG"
     gene            4047..5291
                     /locus_tag="PLM92_00395"
     CDS             4047..5291
                     /locus_tag="PLM92_00395"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HPR23636.1"
                     /translation="MKIGEFANKFGLNASTVRYYINNGLLVPAKKAEQYEFDRDSEED
                     MKNILKYKSYHFTIEEIQLLFFLQKTSRFKDDVVLGLCSDLLKKKRKELMAEKKEMEA
                     HILSLENEIEGMPKTVSPKETKTGIPFSFIPYLYCPVCKEPLKLDSASMAEGTLLSGE
                     LWCECGYNAGIKEGMILCGKHYEDTPFKAFENIESVMAMVDQFSPAYRKLIDQTNLYM
                     YNQIHEDLENPKIIMTGPFTLNFLLAYIDKLNKKNTYIISDPSKKRLEKIKAYLSEGN
                     YNIVYLAGEPGDLPIKEKTADIFIDDYCTVNSLFTYNTFNTENILPLLKPTATVTGIF
                     SSYTKALRGLGNFKKIQPDFRPEKMTFAGLKYGWEQGGFGNIEKKTTGYTSKNEKDYP
                     QDVLGEQVEVMGYTARRGVKRR"
     gene            5288..6067
                     /locus_tag="PLM92_00400"
     CDS             5288..6067
                     /locus_tag="PLM92_00400"
                     /inference="COORDINATES: protein motif:HMM:NF013782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HPR23637.1"
                     /translation="MRKEVFQNEYKMLYSDMDTRLKIKPSRIADIFQELAIQHSAFVG
                     YDLEWFAGNNKGWALLFWHIVINRLPSKHDLVRIGTWARPYKRAQANRSFLMETEKRE
                     ELVKAETRWTLMDTERRRPSKLEPGFFDAYHIPIDPIMPEEKFDMPPCTDVDFVAERK
                     IEVTRRDTDTNGHVNNAVYIEWALDDVPDDIYEKYSLIDIKAAYKRECRKGAVIKCRC
                     YARAESERKTEISTCFTDAENEKTIFSQVSAIWEKPLEAYK"
     gene            6192..6875
                     /locus_tag="PLM92_00405"
     CDS             6192..6875
                     /locus_tag="PLM92_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPR23638.1"
                     /translation="MEILRVENLTKIYGKGESRVKALDDVSFSVDRGEFIAIVGPSGS
                     GKSTLLHMLGGVEKPTSGRVFVDGKDIYEQNDEELAVFRRRQIGLIYQFYNLIPILNV
                     KENISLPFLMDGRNVNESRLDELLEVLDLKDRQDAMPGQLSGGQQQRVSIGRAVINAP
                     AMVLADEPTGNLDSKNSQEIVELLKKSNKEYKQTLFLITHDENIALQAERIIFLDDGK
                     ILREEVAGR"
     gene            6872..9451
                     /locus_tag="PLM92_00410"
     CDS             6872..9451
                     /locus_tag="PLM92_00410"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPR23639.1"
                     /translation="MNPFHKVVLESLKKNKTRTAVIMTGVILSAAMITGVITFASSLQ
                     HFLVEGSIAAKGDWYGMAEPVDKSRKESLEKISKVSETSSYQIKGYSKLDKHGNKYKP
                     YIFVTAFDNEMYDTGMIKMIHGRKPKNPKEIVIPEHLITDGEQNIGIGQKISLGLGER
                     EYIDGKKKKQIYQNVEYMPGKEKFIFEESRTFEIVGICERPATEPYQAAGYTAITGPE
                     KTGEKYGIYIKTEDPNDIYKVIKNIKGEKTQTAYNEELLTYLGAGKNQEINEILFGLA
                     AFLIGLIMLGSIFLIYNAFSVSLSERGRETALLMAIGATRKQLRRSVFYEALLIGGTG
                     IPIGIIAGIAIVGGFLFAAKNLFSGVGNLELSGVTMTLFVTYKTVILAFLVSFVTMII
                     SAMIPALRASRASIINTVRQAYDFRISRNRAKGYSIVSDIFGVYGTLAIKNYSRNTKQ
                     YRVTVFSLLISIILFISVSSYTHYLTSSVDALFGEGEEYQITYSYSGTSPQKGKDNYD
                     ILSNAEGVTKKSFAQVIYMETEITADEIDKKTLNLLRGSGYFKKDKAQLPVRIFVMDE
                     RSYKGYLKNQGLDPKLFTDPENPKAVALKEGGFYDITGQKYRKMNIYGSENVKNLLVR
                     TGEGSGIDEYYNVSIGAFTDEVPAGAGEYAAGFLMVSTPKTADSIFRGYEESIETSTG
                     FFKSDDTKTTYESMAEIASASNIPKSSIINEEEEEEISRSLMTAVNIFSYGFIVLISL
                     IAVANVFNTISTNILLRRREFAILKAVGLSESGFMKMMNLECLFYGIKSLLYGLPIAI
                     IIAYIIYKNVNTGTEIAFSVPVYSVVISIISIFIIVFATMIYATHKIAKEDSIEVIKN
                     EPL"
     gene            9512..9991
                     /locus_tag="PLM92_00415"
     CDS             9512..9991
                     /locus_tag="PLM92_00415"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HPR23640.1"
                     /translation="MKKRNRGRSRKFYKEAQVVDIREAREERRRSRTEIQSKKKKPAN
                     IENPKKKRKKKRLWRRTLIYMGIICIIVLFAGLSVYKIIDLKLEENRLEGQKQELIKE
                     QKKLKEELKNVNDPEYIEQQARKQLKLIMPGETLYVLPDEKDPENEGMSTKDEKEDK"
     gene            9972..10496
                     /gene="rnmV"
                     /locus_tag="PLM92_00420"
     CDS             9972..10496
                     /gene="rnmV"
                     /locus_tag="PLM92_00420"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005885547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HPR23641.1"
                     /translation="MKKKISEIIIVEGRHDTDAINRAVDGYTIETHGFGIKKETWDLI
                     KKAYETRGIIILTDPDHAGMQIRRRIKACFPQAKEAHIKDSEVEDASPEAIVSALSSA
                     RCGREENEEVFTFSDLIRNGLSGNQNAVSKRRDLGDKLGIGYGSTKQFLKKLNLFGIR
                     KEDFHEALQYRKNS"
     gene            10465..11331
                     /gene="rsmA"
                     /locus_tag="PLM92_00425"
     CDS             10465..11331
                     /gene="rsmA"
                     /locus_tag="PLM92_00425"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006441149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HPR23642.1"
                     /translation="MKPYNTGKTPEIFESQGFSHTKSLGQNFLTDKKIRDTIIEAAEI
                     ESTDLIIEIGPGKGVLTNEAARRAAKVIGIEIDKSLIPILDFNLREHDNVKIVNEDIL
                     KCDLGKTIADAGDFAAVKVLGNLPYYITTPIIMKILEENISAKSITVMMQKEVAERME
                     AEPGTKTYGALSLAVQYYCTVKHIANVPKEAFVPRPKVDSRVLRLDIRSRKPVEVQDE
                     KIFFACIRAGFGQRRKTLRNSLAGVTGNDKEKSGRILELTGTDPLRRAETLTMQEFAD
                     IANKLFEEQNKR"
     gene            11328..13310
                     /locus_tag="PLM92_00430"
     CDS             11328..13310
                     /locus_tag="PLM92_00430"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HPR23643.1"
                     /translation="MSKIKTFYRKYLHNVIAAVILLAIVTNLIIETLARQSIWGGFLF
                     FVQSPLIFLYNSLLIFATISLGFLFKRRAAAFVTISFIWIILGIVNGIILSKRMTPFT
                     VYDILSFKEGLSIVGNYFSVLQMVLIAAGIAFVVALFVFLRRKIPKHGKHANHKRRFA
                     VVAVIILITFGVSGLFIRTGIIETYFPNLAYGFRDNGFPYCFINTWFNTGIGKPKDYS
                     KENVRSIFTKKEMETTVAAKGSDGNESHPNILFLQMEAFMDPETVKGLRLSEDAIPTF
                     RKLMKKNSNGRFLSPAVGAGTANVEFEVMTGMSVRFFGPGEYPHKSLLGETVCESVAY
                     DLKNIGYFTHAIHNHRATFYGRNQVFANLGFDTFTSVEYMNKVNRTPKNWAKDYVLTQ
                     QVTDALKATKGPDYIYTISVEGHGAYPEEKVLEDPGITVERSPSEETKYAWEYYVNQL
                     NEMDEFIDELIKKLKVYDEDIVLVIYGDHLPNLPDISDEKLKYGRNTYETDYVIWSNF
                     QMKQKNQDLKAYDISAALLDRLGIKQGTMVTYHQNHKNSRDYQKNLEILQYDMLYGEQ
                     YIYNGKNPFKTTEMKMGVKDIKINKVVKIGEKYYIKGKNFTESSRISLNGKILKTIYL
                     GSTVLGLQEEVDPTDVSKMKVSQFDKEDKEILSTTE"
     gene            13333..13878
                     /locus_tag="PLM92_00435"
     CDS             13333..13878
                     /locus_tag="PLM92_00435"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HPR23644.1"
                     /translation="METIETKRLKLKEWTFDDGPELLALAKDPEVGPKAGWKPHESLE
                     ESIQIINTVFFAGTTWKVTDKKTGRIIGCAGLDHDKKRPEIKSRELGYWLGRDFWNKG
                     FMTEAAKAAIGYGFDKMELDIISVCTAPENKRSQKVIDKLGFSYEGTLRKAYRIYDGT
                     DRDVLCYSITKEEWKNLQNDV"
     regulatory      13974..14055
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     regulatory      14178..14259
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            14398..15237
                     /locus_tag="PLM92_00440"
     CDS             14398..15237
                     /locus_tag="PLM92_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPR23645.1"
                     /translation="MLKRIARVTALILILAVFTSGALAVTADFGYGATSKTSKKVTKK
                     VTKKVIKTKKAARKTYKKTKKTTIKYRTKTIKNTSTQKVTQKKTVVKKVVIKAKKKQK
                     KKKVKTIKTTTTYTTTTAKPAAGTANTTTASQLKATVTPALANAFDKLGFTIKIEPDA
                     RYAGIFSTSKHYIGLQAVSTGVFRHEMGHFLSTLKNKAAKTSTFQAIYQEEKDKYTGT
                     NKAYVTSTSDEYFAESYRNYLENGAKLKTERPKTYSYINQQATSITEDDITRTYNQYS
                     WAW"
     regulatory      15512..15616
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     regulatory      15651..15753
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            15835..>16724
                     /locus_tag="PLM92_00445"
     CDS             15835..>16724
                     /locus_tag="PLM92_00445"
                     /inference="COORDINATES: protein motif:HMM:NF024296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FctA domain-containing protein"
                     /protein_id="HPR23646.1"
                     /translation="MENRRSKKYIAWILAIAMAFTGLGINFSTVYATPGHHLECKKFN
                     LEAYKVLENKDLEAGDFEFVAYKADANGDPTGPVLATGTNDADGNISFSNITVTKDGT
                     YRYVLKEVIPDDEDKIDGVIYDESSKLVTVKVSGNEYVQTISDVIKIDKRQSLYLTTG
                     DANYAVIKQSTFAIVWTEDLLGPNQRAEIKKQVQMWDNSTMNDFYYIHGEGTFLDWGA
                     SRNDDLGHSIPGNAGPYTFSITSDGEIKLECRADRISHLYYGIYGAKATFTNTFLEKG
                     SIDVTKAVKLDGSAWTDFDG"
BASE COUNT         5906 a         3003 c         3654 g         4161 t
ORIGIN      
        1 gggagaacag tattaatgtt ttccaaaaag ggctgaagaa gcaaaaaaat ggataaaata
       61 aaaaaaataa aaccggtaaa atgattatat agcagattct tacgggaaat aaaaagaaaa
      121 aaaagaaaca cggcaagaaa aagctacaat ataaaaagaa ttattttcag aaagcacaga
      181 aaacatatca gtaaaatatc tgaaaagttt gcgggcctca agaagaattt tgtgaaaaag
      241 gaaagcgcat tttcagacac tatggcaggc ctggtctctg aatttgacag aagccacgaa
      301 gaatttgctc aaaaaaccga agccgttgtc agcaaaggga accgcagatt aagatttctc
      361 agagtcatgg cagacagaaa aagagttctg ctgctgaaat actttgcagg tatagtggcg
      421 gtaaccgtag gagtgctgac actgttcaat tatgttaccg gttatgaata ttcatataac
      481 ggaaaggtac ttggtcttgt aaaaggacaa aaagaagtta cccaggtttt ggagatcgcc
      541 agcgatcagc ttaccgatca ttataatgcc aagataacca taggcgaaaa ggaagatata
      601 cagttcagaa aagttgtcac gataaacagg gacgtggacg acatggaaga agtcctaaaa
      661 agacttacat atatgaagga catcaatgcc agcggatacg gcatatatgt aaatgaaaaa
      721 agaatggcag cccttgctac aaagaaagaa gcaaaaaaag ttttaagaga aacactggca
      781 aagttttccc ggaaaaaaga cggagtgaaa tatgaagagg taaaatttgc cgaaaaggta
      841 aaaataaaaa cagtggacac aaaattgggc aatatacaaa aaatagccgc agtgcagggc
      901 aaaatgtttg ctgacgaaga ttcgccaatg ttgcatgtta caactgttga aaaagccgtc
      961 tacaaagaaa aagtgcacta tgaaacgaag aagaaagaca accctgatgc atacataggg
     1021 gaaaactcaa caaccgtaaa aggcaaggac ggagagaatg aagttgttgc caggataacg
     1081 agaattaacg gcaaggaaat aaaaagagat atactgaaaa caaaagaact aaccaaaccg
     1141 gtgacaaagg tggttctggt gggaacaaaa aaaatacccc ccaccatggg agatggtaaa
     1201 tactgcaatc catgtcctgc aggatatgtt tcatcgggat tcggatacag gtggggaaga
     1261 cttcacaggg gcgtggatct ggcttgcagc agcggaaaca gcatatatgc agcggacggc
     1321 ggcacggtgg tatctgcggg atacaggaat tcatacggaa atgttgtaga gataaatcat
     1381 agaaacgggg ttgtgacccg ctatgcccat tgcagcagta ttcttgtaca tgcgggacag
     1441 aaagtgtatg aaggaaagct gatagcgaga gtgggaagca cgggaaatag tacaggacct
     1501 cacttacatt ttgaaatgca gatcaacggc agattcgtaa atccgctgaa gtatatctaa
     1561 ttttcgggtt ttttataaaa ccttatggta aattttgcgc cgccttcaat gcggttttca
     1621 agctttatgc ttccgttctg tttctgaata atcatctttg ccagagcaag cccgataccg
     1681 gtgcttgagt cggtggcatt tttgccttta taaaatcttt caaacagatg aggcatatca
     1741 tcagggtcta tgccgttgcc ggtatcggtt atgattatct ctgtataaag ggcattttca
     1801 tgggcagtaa cttttaagtg cccgccttcg ggggtgtgtt ccatgcagtt tttgagtata
     1861 ttccctagag cctcgcaggt ccagtttata tctcccatga atccgggttt tccggtgact
     1921 tcaagactga aagtttgctg acgtatttcg atggggataa gaagaggatc aaaggatctg
     1981 tcaatcagtt cacgcacatc gacatgctca tttttgaagc cggcagttcc tgcgtctatc
     2041 tttgagattt taagaagaga agatatcagc cagtctatcc ttataaggtt ttgatttatt
     2101 tcctgattgt attccaggcg ctttttttcg gaaaggtctt ttttctgcaa aaaggacatt
     2161 atcagattta ttgtagtaag gggggtccta agctgatgtg atatatcggc aatggaatcc
     2221 gtgaggtata ttttttcttt ttgaagggca tatgaatgtt ctctcagttt gacagtcatt
     2281 ttatatattt cactttcaag gatagccagt tccccttcct gattatcgtc aatagcaaga
     2341 aaatcctgac cgtgaaggat cagattcagt ttttccgaaa gcttctctat ccggtcataa
     2401 cgcttttttg tggaaatcaa aaaaataaga caaaaagaaa cagcaacggc cagtacaaga
     2461 aggctgcagt accaccgcac aaaaaaagca gcaacagtaa ataatgctgt taatataccg
     2521 aagaccgtca tttgccctat tatttctttg tttctcaaaa acttcattac ttctccccgg
     2581 atctgcagaa gcttttatca gctggcagat ttttgtactt ttattccccg actttgtacc
     2641 ccataccgcg aacggttttg attatctcag ggttttgagg gtcatcctcg attttttctc
     2701 taagtctttt tatatatacg gtcaaagtgt tatcatttac gaattctccg gctatatccc
     2761 atatttcttc aagaagtttg ttccttgaaa ggataactcc tttattatta agaaaaacaa
     2821 gaagcagcct gtattccagt acagaaagaa aaatttcctc tcctttcctg tgtacggtgc
     2881 cctttagaga atccactttc agatcaccta tttctattac cggtttggat ttaccgcttc
     2941 ttctcaatat gttttttatt ctggaaacca gttctctggg tctgaagggt ttggtaatat
     3001 agtcttctgc acccatatca agcccggtta cagcagtagc ttcatcatca taggcggtaa
     3061 gaaagatcac aggtgtctct atgttgaggt ttgaaacaga gtttgccttt gccgcagagc
     3121 atacggcata accgctgccg tcaggcagag aaatatcaag aagcaggaga tcgaatttac
     3181 tgttttccaa aagtttcagg gcctgttcct gggtggttga tacttcaact gtgaattcct
     3241 cctcttccag aagcgctttc agattctcga ctatacttat atcgtcttcc accaaaagaa
     3301 ttttattcat tattctgatc gcacagtctt tgataacaaa agccatgcac tcctttcttc
     3361 tatttaagca attttcatta tatcagagcg cgggatatgc gtcaaaaatc tttgaaaagc
     3421 attttacatc atcgtaaaaa agcaaaacaa aatctcccgg cgaaaaaata cgggagatct
     3481 tgctttatac ggggtattcg ggtgcatgta ctatgttttg actttttagt gaagaaatcg
     3541 atcagccgat aacttttttt atggtttcca tgtctttgtt ctcctgaagg cctgccagta
     3601 cgggttcctt ttcaaagcct tgtttttttc cttcaagctt ggcaagacat gattctatca
     3661 aatctacact tctttcagaa ggagctccgc cgtcaaccag aaaggggatg ctgcggtaat
     3721 agtttttaac aggcattctt gtggggtcaa ccagcatttt gccgccactt ttggcaagct
     3781 ctcttgcttc ttcaaaaata tcgatagctg tagcgttgaa atattttaca tcggcctttc
     3841 cttcaaatgc ttcttttact tttttgttgt tggttactat aactcttttc attttcatta
     3901 cctccttgga tttctttatt tgtctgtatg atatactgtg aaccggtctt tacggcaata
     3961 tacaaatttc aaaaaaagtg aacccgggtt catcttgacc aaaaaacacc tttaacatat
     4021 actgtagttc ataggagaag cgtaaaatga agataggaga atttgcaaat aaattcggac
     4081 ttaatgcaag tacagtaaga tactatataa ataacggact tctggtaccg gcaaaaaaag
     4141 cagagcaata tgaatttgac agagacagtg aagaggacat gaaaaacatt cttaaatata
     4201 agagctatca ttttaccatc gaagaaatac agcttctgtt ttttttgcag aaaacttcaa
     4261 gattcaagga cgatgttgta ctgggattgt gttcagacct tcttaaaaag aaacgaaaag
     4321 aacttatggc cgaaaaaaaa gaaatggaag cacatattct ttccctggag aatgaaatag
     4381 aaggaatgcc aaaaacagtt tctccgaaag aaacaaaaac cggcataccc ttttccttta
     4441 taccatatct ttactgtcct gtctgcaagg agccccttaa acttgattct gccagcatgg
     4501 cggaaggaac ccttctttcg ggagaactct ggtgcgagtg cgggtacaat gccggcatca
     4561 aggaaggaat gatactgtgc ggtaagcatt atgaggatac gcccttcaag gcctttgaaa
     4621 atattgaatc ggtaatggca atggtggatc aattcagtcc tgcctacaga aaactcatag
     4681 atcagacaaa tctttatatg tacaatcaga tccatgaaga tcttgaaaat ccaaagatca
     4741 taatgaccgg accctttacg ttgaatttcc tgcttgcata tatagacaaa cttaacaaaa
     4801 agaacacata tataatatcg gacccctcta agaaaaggct tgaaaaaata aaggcatatc
     4861 tttcagaagg aaattataac atcgtatatc tggcgggaga accgggagat ctgcccataa
     4921 aagaaaaaac ggcggatatc tttatagatg attactgtac tgtcaattca ctttttacat
     4981 acaatacttt caatacagaa aatattttac cgttgcttaa acccactgca actgtaacag
     5041 gtattttcag cagttatact aaagccctca gggggctagg caatttcaaa aagatacaac
     5101 ctgatttcag acctgagaaa atgacctttg ccggactcaa atatggatgg gaacagggtg
     5161 gattcggcaa tatagagaaa aaaaccaccg gatacacatc aaagaacgaa aaggattatc
     5221 cccaggacgt tttgggagaa caggtagagg taatgggata tacagcaaga agaggagtca
     5281 aaagaagatg agaaaagaag tttttcaaaa tgaatataaa atgctttatt cggatatgga
     5341 cacaagatta aaaataaaac cttcccgtat agcggatatt tttcaggaac tggcaataca
     5401 acattccgcc tttgtgggat atgacctgga atggtttgcg ggcaacaaca aaggatgggc
     5461 tcttcttttt tggcatattg taataaacag attgccctca aaacacgatc ttgtaagaat
     5521 aggaacatgg gcaaggccgt ataaaagagc ccaggcaaac agaagttttc ttatggaaac
     5581 agaaaagaga gaagaacttg taaaagcaga gacaagatgg actcttatgg atacagaaag
     5641 gagaagaccc tcaaaactgg aaccgggatt ttttgacgca tatcacatac ccattgatcc
     5701 gatcatgccg gaggaaaaat ttgatatgcc cccttgtacg gatgtagatt ttgttgcaga
     5761 gagaaaaata gaggtcacaa gaagagatac agatacaaac ggacatgtaa acaatgcagt
     5821 ctatatagaa tgggcccttg acgatgtgcc ggatgacata tatgaaaagt acagtctgat
     5881 cgatataaaa gcggcataca aaagagaatg cagaaaagga gctgtcataa aatgccggtg
     5941 ttatgccaga gctgagagcg agagaaaaac ggagataagc acctgcttta ccgatgcaga
     6001 aaatgaaaaa accatttttt cacaggtgtc tgccatatgg gaaaaacctt tagaggctta
     6061 caaataatca gtattataag atactatgaa acttacacta ttgttattgt gatatcacat
     6121 caaaaacaca taaaaaatat ataatagcac acgccgtttg aaagaacggt tttttggggg
     6181 aaaaagaaaa aatggaaata cttcgtgttg aaaacctgac aaaaatatac ggcaaaggcg
     6241 agagccgggt aaaagcgctt gatgatgttt cattttcggt ggatagagga gaattcatag
     6301 caatcgtagg gccttccgga tccggtaaat caacattgct gcatatgctg ggaggtgtgg
     6361 aaaaacccac ttccggcagg gtttttgtgg acggtaaaga tatatatgag caaaacgatg
     6421 aagaactggc tgtgttcaga agaaggcaga taggacttat atatcaattt tacaacctga
     6481 tacccatatt gaacgtgaaa gaaaacatat cccttccttt tctgatggac ggaagaaatg
     6541 taaatgaaag ccgccttgat gaacttcttg aagtccttga tctgaaagac aggcaggatg
     6601 ctatgcccgg acagctttca ggagggcagc agcagagagt atcgatcggc agagcggtga
     6661 tcaatgctcc tgcaatggtt ttggccgatg agcctacagg aaatctggac agcaagaaca
     6721 gtcaggaaat agttgaactg ctcaaaaaat ccaacaaaga atataagcag actctttttc
     6781 ttataaccca cgatgaaaac attgcacttc aggcggaaag aataatcttt cttgatgacg
     6841 gtaaaatact tcgtgaagag gtggcaggac gatgaacccc tttcataagg tcgttttaga
     6901 atcccttaaa aaaaacaaga caaggacagc ggtcataatg acaggtgtca tactttctgc
     6961 tgccatgata acgggagtga tcacctttgc cagcagtctg cagcactttc ttgttgaggg
     7021 aagtattgca gcaaagggag actggtatgg catggctgag cctgttgata aaagcagaaa
     7081 agaaagttta gaaaaaatct ccaaagtttc ggaaacatca tcatatcaga taaagggata
     7141 ttcaaaactt gataaacatg gcaacaaata taaaccatat atctttgtaa cggcttttga
     7201 caatgaaatg tacgataccg gcatgataaa aatgatacat ggcagaaagc caaaaaatcc
     7261 aaaggaaata gtaataccgg aacatttgat aacagacggg gaacagaata tcgggatcgg
     7321 acagaaaata tcattgggtt tgggagagag agaatatatc gacggcaaga aaaaaaagca
     7381 gatatatcag aatgtggaat acatgcccgg gaaagaaaaa tttatttttg aagaaagccg
     7441 cacattcgaa atagtaggca tatgtgaaag accggcaaca gagccttatc aggcagccgg
     7501 gtatacggca ataaccgggc cggagaagac cggcgaaaaa tatggcatat acatcaaaac
     7561 cgaagatcca aatgacatct ataaagtcat aaaaaatatc aaaggagaaa aaacacagac
     7621 cgcatacaat gaagaacttc tgacatatct cggagcaggg aagaaccagg aaataaacga
     7681 aatccttttt ggtcttgcag cctttcttat cggcctcata atgctgggat ctatatttct
     7741 tatatataat gctttttctg tttcattgag tgaaagagga agggagaccg cattgctgat
     7801 ggccatagga gcaaccagaa agcagttgag aagatctgtt ttttacgaag cacttttgat
     7861 cgggggcaca ggcattccca tcggaattat cgcaggaata gctatagtcg gagggttcct
     7921 tttcgcagca aagaatcttt tttccggagt ggggaatctg gagctgagcg gtgtcaccat
     7981 gacactcttt gttacatata aaactgtaat actggcattt cttgtttcct ttgtcactat
     8041 gatcatatct gccatgatac ctgcgctcag agcttccagg gcatctataa taaatacagt
     8101 aaggcaggct tatgattttc gcataagccg aaacagagca aagggataca gtatagtttc
     8161 tgatattttc ggggtttacg gtactcttgc catcaaaaat tacagcagaa atacaaaaca
     8221 gtaccgagtg acggtatttt ctctgctcat aagcataata ctttttattt ccgtcagttc
     8281 gtatactcat tatctgacaa gcagcgttga tgcattgttc ggtgaaggtg aagagtatca
     8341 gataacatac agttattcgg gcacttctcc tcaaaaagga aaagacaatt acgatatcct
     8401 ctcaaacgcg gagggagtaa caaaaaagag ttttgctcag gtcatttata tggaaaccga
     8461 aataaccgct gatgaaatag ataaaaaaac actgaatctt ttgaggggat ccgggtattt
     8521 caaaaaggac aaagcacagc tacctgttcg gatctttgta atggacgaaa gatcctataa
     8581 agggtacttg aaaaatcagg gattggaccc caaactgttt acagatccgg aaaatcccaa
     8641 agcagtagct cttaaagaag gtggattcta tgacataacg ggacaaaaat acaggaagat
     8701 gaacatatat ggatcagaaa atgtaaaaaa cctgttggtc cggacaggtg aaggaagcgg
     8761 tattgatgaa tattataatg tttctatagg agcatttacg gatgaagttc ctgccggtgc
     8821 cggagagtat gctgcaggat ttctgatggt gtccactcca aagactgcgg acagcatttt
     8881 cagggggtat gaagaaagca tagaaaccag tacaggattt tttaagagcg atgacacaaa
     8941 aaccacatat gaatcaatgg cagagatagc ttctgccagc aacatcccca aaagcagtat
     9001 tataaatgag gaagaggaag aagaaataag cagaagcctt atgacagccg ttaacatttt
     9061 ttcctatggg tttatagtac tcatatccct tattgcggtg gccaatgttt tcaatactat
     9121 ttctaccaac atacttctaa gaagaaggga attcgccata ctgaaagctg ttgggctaag
     9181 tgaatcggga ttcatgaaaa tgatgaatct ggaatgcctg ttttatggga tcaaatcttt
     9241 gctttacgga cttcccattg cgatcattat tgcatatata atatataaaa atgttaatac
     9301 aggaacagaa atagcttttt ctgttcctgt atacagcgtg gtgataagca taatcagcat
     9361 attcattatt gtttttgcaa cgatgatata tgcaactcac aagatagcaa aagaagactc
     9421 tattgaagtg atcaagaatg aaccgctgta gagaaatttg ttttttgtga tataatatca
     9481 gcggcagttg tattttttat aaaaggaaaa tatgaaaaag agaaatcgcg gaagaagcag
     9541 gaaattctat aaagaggctc aggtagtcga tatccgtgaa gcaagagagg aaagacgcag
     9601 gtcccgaaca gaaatccaaa gcaaaaagaa aaaacctgca aatattgaaa accctaagaa
     9661 aaaacggaag aaaaaaagac tttggcgccg cacattgata tatatgggga tcatttgtat
     9721 cattgttttg tttgcgggcc tttcggttta caaaataata gacctgaagc tggaagaaaa
     9781 cagactggag ggacagaagc aggaactgat aaaggaacag aagaaactga aagaagaatt
     9841 aaagaatgtc aatgatcccg agtatataga acagcaggca agaaaacagc ttaaactaat
     9901 aatgccggga gagacccttt atgtacttcc tgacgaaaaa gatccggaaa atgaaggtat
     9961 gagcacaaaa gatgaaaaag aagataagtg aaataattat tgtggaaggc cggcatgata
    10021 cggatgccat aaacagagcc gtggacggat ataccataga aacccatgga ttcggcataa
    10081 aaaaagaaac atgggacctt ataaaaaaag catatgaaac cagggggata ataatactca
    10141 cggatcccga tcatgcgggg atgcagatca gaagaaggat caaagcctgt tttccccaag
    10201 cgaaagaagc acatataaaa gactcggagg tggaggacgc ttctcctgag gctattgttt
    10261 ccgctctgtc atctgcaaga tgcggcagag aggagaatga agaggttttt acattttccg
    10321 atctgataag aaacggcctg agcggcaatc aaaatgcagt atcaaaacga agagacctgg
    10381 gagataaact ggggatagga tatggaagca cgaaacaatt tctcaaaaaa ctcaacctgt
    10441 tcggcataag aaaggaagac ttccatgaag ccttacaata ccggaaaaac tcctgaaata
    10501 tttgaaagtc aaggattcag tcatacaaaa agcctgggcc agaattttct tacggacaaa
    10561 aaaatacgtg acactataat tgaagcagca gagatcgaaa gcacagacct tataatagaa
    10621 ataggtccgg ggaagggggt gcttaccaat gaagcggccc ggagagccgc aaaggtaata
    10681 ggaatagaaa tagacaaaag tcttataccg atacttgatt ttaatcttcg agaacatgat
    10741 aatgtcaaaa tagtaaatga agatattcta aaatgcgatc ttggaaaaac aatagcagac
    10801 gcaggagatt tcgccgcagt aaaggttttg ggcaatcttc cctactatat tacaacgccc
    10861 ataataatga agatactaga agaaaacata tccgcaaaaa gcataaccgt aatgatgcag
    10921 aaagaagtgg cggaaagaat ggaagcagag ccgggaacaa aaacatatgg agctctttct
    10981 ctggccgtac agtattactg cacagtcaag catatagcaa atgttccaaa agaggcattt
    11041 gtgccaagac cgaaagtgga ttcaagagtt ttgagacttg atataagaag ccgtaaacct
    11101 gttgaagttc aagatgaaaa aatatttttc gcatgtataa gagcaggatt cggacagaga
    11161 agaaaaaccc tgcgaaattc cctggccggt gtaacgggaa atgataagga aaaatccgga
    11221 agaatacttg agcttacggg cacagatccc ttaaggagag ccgagaccct tacaatgcag
    11281 gagtttgcag atatagcaaa taaactattt gaggagcaaa ataaaagatg agcaaaataa
    11341 agacttttta tcgaaaatat ctgcacaacg tcattgccgc agttatttta ctggcgatcg
    11401 ttacaaattt gataattgaa acacttgcaa ggcagagcat atggggagga tttctgtttt
    11461 ttgttcagag ccctttgatc tttttgtata attctctttt gatctttgca accatttctc
    11521 tgggattttt gttcaagaga cgggcggccg cttttgttac gatctctttc atatggataa
    11581 ttttaggcat agttaacggc ataatacttt caaaaagaat gactccgttt actgtttatg
    11641 acatactttc atttaaggaa ggactttcca tagtcggcaa ttatttttct gttttacaaa
    11701 tggtcttaat agcagcaggt atagcctttg tggttgctct gtttgttttt ttgagaagaa
    11761 agatcccaaa gcacggaaaa cacgcaaatc acaaaagacg ttttgcggtg gtggcagtaa
    11821 ttatactgat caccttcggt gtctccggat tgtttataag aacagggatc atagagacgt
    11881 atttcccgaa cctggcatat ggattccgcg ataacggatt cccttactgc ttcataaaca
    11941 catggttcaa tacggggata ggaaagccaa aagactattc aaaagaaaat gtcagatcca
    12001 tatttacaaa aaaagaaatg gaaacaacag tagccgcaaa gggaagtgac ggaaacgaaa
    12061 gccatccaaa cattcttttt ctgcagatgg aagcttttat ggatcctgaa acagtaaaag
    12121 gtctcaggct ttccgaagat gccataccca ccttcaggaa acttatgaaa aaaaattcca
    12181 atggcagatt tctttctccg gcagtgggag cgggaacagc caatgtggag tttgaagtca
    12241 tgaccggaat gagcgtccgt tttttcggtc cgggagaata tcctcataaa tcattgctgg
    12301 gagaaactgt ctgtgaaagt gttgcttatg atttaaaaaa tataggctat tttacccatg
    12361 caatacacaa tcacagggca accttttatg gcagaaatca ggtttttgct aatctggggt
    12421 ttgatacctt tacttccgtt gagtatatga acaaagtaaa cagaacacca aaaaactggg
    12481 ccaaagacta tgtccttacg cagcaggtca ccgatgcact caaagcaaca aagggcccgg
    12541 attacattta tacaatatcc gtagaaggtc acggtgcata cccggaagaa aaggttcttg
    12601 aagatccggg aataacagtt gagagatccc catcggagga aacaaaatat gcatgggaat
    12661 actatgtgaa tcagcttaat gaaatggacg aatttataga cgaacttatc aaaaagctta
    12721 aggtatatga tgaagacatc gtacttgtca tatacggaga tcatctgcca aacctgcctg
    12781 atataagtga tgaaaaactt aaatacggca ggaacacata tgagacggat tatgttatct
    12841 ggagcaattt ccaaatgaag caaaaaaatc aggatcttaa ggcatatgat atatcggcag
    12901 cgttacttga cagacttgga ataaagcagg gcacaatggt aacatatcac cagaatcata
    12961 aaaacagcag ggactatcaa aagaatcttg aaatcctgca gtatgacatg ctttacggag
    13021 aacagtatat atacaacggt aaaaatcctt ttaaaaccac agaaatgaaa atgggcgtca
    13081 aagatataaa aataaacaag gtcgtgaaaa taggagaaaa gtattatata aaaggaaaga
    13141 actttaccga atccagcagg atctctctaa acggcaaaat actcaaaacc atatatcttg
    13201 gatctaccgt gctggggctc caggaagaag tggatccaac ggacgtatcc aaaatgaaag
    13261 taagccagtt cgacaaagag gacaaagaga tcttaagcac aacggaatag gaacggtaaa
    13321 gaggtggtca aaatggaaac cattgaaaca aaaagattga aacttaaaga gtggaccttc
    13381 gatgacgggc cggagctttt ggcactggcc aaagatccgg aagtcggtcc caaagcaggc
    13441 tggaaacccc acgaaagcct tgaggaatca atacaaataa tcaacacagt cttttttgcc
    13501 gggacaacat ggaaagtgac agataaaaaa accggaagga taatcggatg tgcaggtctt
    13561 gatcatgaca aaaaaaggcc tgaaataaaa agcagagaac tgggatactg gctgggcaga
    13621 gatttctgga acaaaggttt catgacagag gcggcaaagg ctgcaatcgg ttatggattt
    13681 gataaaatgg aacttgatat catttccgtc tgtacagccc cggaaaacaa aaggtcgcag
    13741 aaggtaatcg acaaactggg attttcttat gaaggcacat taagaaaagc ttacaggata
    13801 tatgacggaa cggacaggga tgtgctttgc tattccatta caaaggaaga atggaaaaat
    13861 ttacagaatg atgtttaagg tccgttttaa agggtataac aaagatataa ttggaaaaga
    13921 aaaaatatac ttgacaaaga aaaaaatgca gtttatagta tatttagaaa agtattcagt
    13981 aatggcaaag caggagaaat cctgtgacgc aaagctaagg ggctaaaccg taaggtatgc
    14041 cagccagctg ccgcattaca aaatgtgtat ggatattaca acactaatgc gtgaactgcc
    14101 attcggatac ccccgaatgg ctttttttat ccgtaaaccg agcacgcgcc cggtggagtg
    14161 atccgtacaa aaactaaata ttgattgggc aaagcaggag aaatcctgtg acgcaaagcc
    14221 aagggtctaa accgcaaggt atgacagccg gttgccgcag atagttcgca gctatcagcg
    14281 ataagagaaa aagcatttta tgtgcctttc gaaaagcaac gactgtcaaa acccgacagt
    14341 cgtttttagt ttatcaaatg tattttgaaa aacaaattta ttcggaggta cggaacaatg
    14401 ttaaagagaa tagcaagagt gacagcactc atattgatat tagcggtttt cacaagcggt
    14461 gcgcttgcgg taactgctga ttttggttat ggagcaacca gcaagacaag caaaaaagta
    14521 accaaaaaag taaccaaaaa agtcataaaa acaaaaaaag cagcaaggaa aacatataag
    14581 aagacgaaaa aaacaaccat aaaatacaga acaaaaacaa taaaaaacac gagtacccag
    14641 aaagtaacac aaaaaaagac agtggtcaaa aaagtagtta tcaaagcaaa gaaaaagcag
    14701 aagaaaaaga aagtaaaaac cataaaaact actactacat atacaactac aacagcaaaa
    14761 cctgccgcag gtacagcaaa tacaacgacg gcaagccaac tgaaagcaac agtgaccccg
    14821 gctctggcaa atgcatttga caaactgggt ttcaccataa agatagaacc cgacgcaaga
    14881 tatgccggta ttttcagtac ttccaaacat tacataggac tgcaggctgt aagcacggga
    14941 gtattcagac atgagatggg tcatttctta agcacactga aaaataaagc cgcgaagacc
    15001 agcactttcc aggccatata tcaggaagag aaggacaagt atacaggaac caataaggcc
    15061 tacgttacaa gtacatcgga tgagtatttt gcagagtcct acaggaatta cctggagaac
    15121 ggagcaaaac taaaaacaga aaggccaaag acatacagct atataaatca gcaggcaacc
    15181 agcataacag aagatgacat aacaaggacc tacaatcagt acagctgggc ctggtaaaaa
    15241 caagtcaaaa gggcaagaat ttgcttacaa aatacaagaa ggaaacccga ataactgcga
    15301 atatttgggt ttttttcttt tttggatcaa agaaaaaatg caaaatcatg ataaaaaaag
    15361 cccttggatt attacaaaaa acttttcccc caaaattttt aaaagagccc tttttcaaaa
    15421 aaaacttgac atttatcctt aaaaggcgta tttttttagt agggaaagag attatgagac
    15481 aactacaggc ggatatttta ttgtctcaag gaatcgataa aggcaaagca gtcgtgaggc
    15541 tgtggcgcaa aactacaggg tcttggggaa aaacaaaaat aagtgacata attaaccaag
    15601 atagccagct gccgattata ataataggca gcttttttaa tagctgcatg taatgaaaag
    15661 ggcaaaacag tcgtgaggct gtggcgcaaa actatagggt cttgggaaat agtatcaatt
    15721 cagggatcaa tgccaagata gccagttacc gttacttccg aaaaaaacgg taactttttt
    15781 atttgcggtc ccggcaaaaa aaagttacga actaaacgga aggagaatgg gaaaatggaa
    15841 aataggagga gcaaaaaata catagcctgg atactggcaa ttgcaatggc atttaccggt
    15901 ctgggaatca acttttctac cgtgtatgca acacctgggc accatctgga atgtaagaag
    15961 ttcaatctgg aagcatataa ggttcttgaa aacaaggatc ttgaagccgg ggactttgag
    16021 tttgttgcat ataaagcaga tgcaaacgga gatcctacag gaccggttct tgccacaggg
    16081 acaaatgatg cggatggcaa tataagtttt tcaaacataa cagtgaccaa agacggtaca
    16141 tacaggtatg tactgaaaga agtcatacca gatgacgagg acaaaataga cggtgttata
    16201 tatgacgaaa gcagcaagtt ggttaccgta aaagtatcag gtaatgaata tgtgcaaaca
    16261 ataagcgacg tgatcaaaat tgataaaaga caaagtctat atttaactac aggtgatgca
    16321 aattatgcag taataaaaca atccacattt gccatagtat ggacggaaga tctacttggt
    16381 ccaaatcagc gtgcagagat aaaaaaacag gtccagatgt gggacaattc gacaatgaat
    16441 gatttttact acatccacgg cgaaggaaca tttttagatt ggggagccag cagaaatgat
    16501 gatttgggac attccatacc aggcaatgca gggccctata cattcagcat aacctcagat
    16561 ggtgagataa agcttgaatg ccgagcagac aggataagcc atttgtatta cggtatctat
    16621 ggcgcaaaag ccacctttac aaatacattc cttgaaaaag gcagtataga tgtaacaaag
    16681 gcggtgaaac tggacggaag cgcatggaca gactttgacg gaga
//
LOCUS       DAONHK010000005        16295 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_2803_length_16295_cov_5.000123, whole genome shotgun sequence.
ACCESSION   DAONHK010000005 DAONHK010000000
VERSION     DAONHK010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 16295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16295
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2803_length_16295_cov_5.000123"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /locus_tag="PLM92_00450"
     CDS             complement(<1..578)
                     /locus_tag="PLM92_00450"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF012901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HPR23647.1"
                     /translation="MKIGIVFSGQGAQHPGMGKDLYDNHKTAREIFDAAGKQIKEWCF
                     NGTAEMLRQTYVTQPSIYVVTMAIYRVFKEEAAKEGLDKTLELTGIAGFSLGEYSALT
                     VAGTIDEIGKGIDIVKKRGVFMQEAGTDINGDPKGGMVAAFGNREKILGIIDQISEGR
                     ILEAVNFNSPVQTVVAGENKALEEFTEEASNN"
     gene            complement(511..1503)
                     /gene="fabK"
                     /locus_tag="PLM92_00455"
     CDS             complement(511..1503)
                     /gene="fabK"
                     /locus_tag="PLM92_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HPR23648.1"
                     /translation="MIMTGQNRVCQILGTEYPIIQGAMALIAESSLAGAVSRGGGLGI
                     IASGGAQGSWVVNEIKKIRAMTENPFGVNIMLLDPNVEEIIEIVCREKVPVVTTGAGN
                     PGKYIKRLKENGIAVLPVVANQTLAKRVERAGADAVIAEGCEAGGHIGEITTMAMIPQ
                     MADSLSIPVIAAGGIGDGRGIAAAYMLGAEGVQVGTRFLTSEECIVARNYKDAILKAR
                     DSSTVATGRSTGHPVRVIKNKLARQVLEMEKNNAEAEEINEVCTGSLRRAVFSGDMEM
                     GSVMSGQIAGLVNKEQSAAEMISEMFKEAAEIYEDRHSIFRTGSSASGDGQGSI"
     gene            complement(1500..3140)
                     /locus_tag="PLM92_00460"
     CDS             complement(1500..3140)
                     /locus_tag="PLM92_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008402534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPR23649.1"
                     /translation="MNDLIKKYITVCEDANGVLQSISFTNNEKFNFAYDIVDELARIK
                     PQKRAMLYVDKDKNERNFTFGDLKVLSDKTANYFSCLGIKKGDRVMLVLKRHYQFWLA
                     VLALHKLGAVIIPATHQLKEEDFNFRFDTGDINAVFCTHEDDVTEEADKSIAGGKRDV
                     IRIMTGPAKEGWHSFDAEMEGFSEEFQRRETRGDDPMAMFFTSGTTGYPKIVTHSFKY
                     PLGHYVTARYWQNVDPEGLHFTLSDTGWAKSLWGKIYGQWMCEAAVFIYDMEKFVPHD
                     FLSLFARFGITTFCAPPTAYRILVKRNLGKYRLSELRHVTTAGEALNPKVWQEFYDKT
                     GLKIMEGFGQTETTLTVCNLLGTEPRPGSMGKPSPAYDVDIVDEKGNTAKTGETGEIV
                     IRTDKSVPCGLFTGYYGNEERTGDCWHDGIYHTGDTAFRDEDGYFWYVGRTDDLIKSC
                     GYRVGPFEVESVLMELPYVLECAATGLPDKTRGQIVKASIVLVEGTKGDRSLEKEIIE
                     YCRQHMAHFKCPRKVEFLDEMPKTISGKIRRTELRGDK"
     gene            complement(3146..3379)
                     /gene="acpP"
                     /locus_tag="PLM92_00465"
     CDS             complement(3146..3379)
                     /gene="acpP"
                     /locus_tag="PLM92_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008594933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HPR23650.1"
                     /translation="MMTFDKIKTIIAEQLSADEEKVKMETHLMKDLEADSLDAVEIIM
                     RIEEEFEIEISDEDAEKMQYVKDLVKYVDEKSM"
     gene            complement(3612..3812)
                     /locus_tag="PLM92_00470"
     CDS             complement(3612..3812)
                     /locus_tag="PLM92_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPR23651.1"
                     /translation="MGIFDFDVDGDGEFSALDMEIVGELDDASGDNALLIERAYELGL
                     DPEEYDSAEELQEAVDEAEEAQ"
     gene            complement(3898..4815)
                     /gene="tsf"
                     /locus_tag="PLM92_00475"
     CDS             complement(3898..4815)
                     /gene="tsf"
                     /locus_tag="PLM92_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006001731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HPR23652.1"
                     /translation="MSVTASMVKELREITGAGMMDCKNVLMETDGDIEKAVELLREKG
                     IAKAAKKADRIAAQGLVRTAFSKDGKTAAIIEVNSETDFVAKNQEFIDFVDSLAKLVM
                     ENDVKSKDEFMDMPFEGSTVQEVLKSKIAKIGENMNIRRFEKFCEADTVYKGYIHGNG
                     TIGVIVGIKTEASGEETEEAGKDVAMQVASMNPRFLDGNDVDPEFIAEEKKILTQQVL
                     NEGKKPEMVDRIVEGKLKKELKEYCLLEQKFVKDSEVTVKQYIENTAKAKGKEMEVTS
                     FVRFEVGEGIEKKEEDFAEEVAKQMDMNK"
     gene            complement(4816..5628)
                     /gene="rpsB"
                     /locus_tag="PLM92_00480"
     CDS             complement(4816..5628)
                     /gene="rpsB"
                     /locus_tag="PLM92_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002472447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HPR23653.1"
                     /translation="MSVISMKQLLEAGVHFGHQTRRWNPKMAPYIFTERNGIYIIDLQ
                     KTVKKIEEAYEFMRTVGESGKPVLFVGTKKQAQAAIQDEALRCGMFYVNQSWKGGTLT
                     NYKTISSRIKRLFDIQKMEEDGIFQKLSKKEVLTLRNEAEKLERFYGGIKDMKGMPGA
                     LFVVDPKKEANAVREARILGIPVIGIVDTNCDPDDVDFVIPANDDAIRAVKLIASSMA
                     DAIIESNQGESFDEGYEEETEEAETAEIEATGEKTEEASESEEDVSEESEGK"
     gene            complement(5771..6160)
                     /locus_tag="PLM92_00485"
     CDS             complement(5771..6160)
                     /locus_tag="PLM92_00485"
                     /inference="COORDINATES: protein motif:HMM:NF016296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HPR23654.1"
                     /translation="MKNDDIMQYEMRKKRIVVIKKKETARIPQNEILFIEQRERKIII
                     VTAKETFTYYGKMKECWPVLSGCFHRCHTYLAVNLDNVRRIADGAIYFFDSKEPVFLG
                     RAAYSKTKKGFNTYLIKAHAEEIPCKY"
     gene            complement(6254..6472)
                     /gene="rpoZ"
                     /locus_tag="PLM92_00490"
     CDS             complement(6254..6472)
                     /gene="rpoZ"
                     /locus_tag="PLM92_00490"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HPR23655.1"
                     /translation="MLYPSINEIRKKADSRYTLVILAAKRARDIIDGKPILIEGTDEE
                     GRDFSEERPVSVAANEIAEDYITYKRQE"
     gene            complement(6472..7083)
                     /gene="gmk"
                     /locus_tag="PLM92_00495"
     CDS             complement(6472..7083)
                     /gene="gmk"
                     /locus_tag="PLM92_00495"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003869229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HPR23656.1"
                     /translation="MSRGRLFIISGPSGAGKGTICTRLLEETEKLFFSVSMTTRKPRE
                     GEIEGRDYYFVSEKKFLEMKAGDGFLESAYVYQDYYGTPKQMVMEKLDSGNDVVLDID
                     IQGAMSIKKVYPYGVFIFILPPSMAELRERITGRGTEKNESIEVRMAGAMKEIAYIDK
                     YDYCVVNGELEEAVCRVKAIIKAEHSRVSEDIYELIEKYKEEI"
     gene            complement(7165..8049)
                     /locus_tag="PLM92_00500"
     CDS             complement(7165..8049)
                     /locus_tag="PLM92_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006001725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HPR23657.1"
                     /translation="MIKSMTGFGKGEYSDGKRIITAEVRSVNHRYSDINVKMPRRYNF
                     AEDKIKNIVKETVKRGKVEVAILIEAVTEDDLKISLNTAAASQYHKNLNKLKEEFGLP
                     GSIDLALISGMPEVMKTIPDVEDEEEILRSVEIAVRDAVDAFDSMRTKEGAKLAEDLA
                     MRAAKVRDDLKKIEKKAPQVSKTYAIKLAERIEELIGKEIEIPKDRILLEAAIFADKA
                     NITEELVRLDSHMIQMDDIISKSDRSDGKKLDFLVQEMNREANTIGSKANDMEITGIM
                     LEIKSEVEKIREQVQNIE"
     gene            8127..9824
                     /locus_tag="PLM92_00505"
     CDS             8127..9824
                     /locus_tag="PLM92_00505"
                     /inference="COORDINATES: protein motif:HMM:NF017633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT RNA binding domain-containing protein"
                     /protein_id="HPR23658.1"
                     /translation="MAFDGIFTGAAAFELNEKLRGAKVEKIYQPERDELVFHLHCPSG
                     DHRLYASCSSNHPGVYLTSEKFQNPASPGSFCMLLRKHIGKSHISDIHQKDSERIIEI
                     LFTGRDEMGYGKNKKIIFEIMGKHSNIILVNADDNIITDSIKRISLGMSRIRPVLPGR
                     PYEYPPAQDKISLFSVTLSDVNELCRENDISRALLSRIQGISPLISDELSMEKSSDAV
                     FKKICMIRESVKNGNFASAGWLKENGEPRDFHVIPVSAYEELDIIRFENPSEAVSWYY
                     KNKDSANRIRQKSSDLNKIISAALKKLRLKKQRLREDLLYAEESKKFRLYGELITANI
                     HRIKQGENSAVVFDYNNGEEITIPLDVRLSASQNAQIYFKKFRKSKTALVEKQKQLSE
                     TDSDITYLESTAEHLNRSEKVSDIDAIRDELVETGFIRKRRDLPAKKIKAEPFSYVSS
                     EGYVILAGRNNRENDFLTFRKASRTDLWFHTKDIPGSHVIMLTEGREPGENSILEAAA
                     LAAYHSKGASSENVPVDYTQIRYVKKPSGAKPGMCIFTNNKTLYVKPSVPVSILSSS"
     gene            complement(9784..10641)
                     /locus_tag="PLM92_00510"
     CDS             complement(9784..10641)
                     /locus_tag="PLM92_00510"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HPR23659.1"
                     /translation="MFENFPIKGSGEKDRITGYLWAKEEPDYVICIIHGIGEHAGRYD
                     RMNKFLSEANIACLSMDLKGHGASSGKRGHCAPRESVLGDIDILLEHAMKKYPNKPVI
                     LYGHSLGGNIALDYRARGKLNDKPFAYIISAPWIELVNKPAAPLFLFVKIMARICPEM
                     TIKSSVNEEDLGHPLSVQPYKTNPMVHDKISMLCAFEAFEKGEALAKNENEDNGRSKG
                     QPLLVMHGTEDRVCSIEGSRKFVSHEKEACTYIEWEGMFHEIHNGNKENDGTEVIMKM
                     IEWIRGLKA"
     gene            complement(11028..11960)
                     /locus_tag="PLM92_00515"
     CDS             complement(11028..11960)
                     /locus_tag="PLM92_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004842895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HPR23660.1"
                     /translation="MKEEKIFNITADQETDGTRLDKVLSFSLPETSRNHIQKLIEKGM
                     VSLGGEVCFSKKHKVKAGEKIRILLPESEETNIVPEDIPLDIVYEDDDLLVIDKQKGM
                     VVHPAAGSYSHTLVNAVLFHCGSSLSGINGIARPGIVHRIDKDTTGLIMIAKNDFAHR
                     SLARQLAEHSVTRGYKALVYSNIVEDEGRIEEPLGRDPKNRLRQTVGGLNAREAVTNY
                     KVIERFGKYTLIEARLETGRTHQIRVHMAYIKHPLFGDYIYGPKKNRYGIEGQMLHAY
                     LLGFMHPSRAEYIEFKSPLPNEFETVLEKIRNEK"
     gene            complement(11938..12399)
                     /gene="lspA"
                     /locus_tag="PLM92_00520"
     CDS             complement(11938..12399)
                     /gene="lspA"
                     /locus_tag="PLM92_00520"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HPR23661.1"
                     /translation="MYYLIIAAIVLLDQMIKALAYSGLDPAHPVTLLDGVLYLTYVEN
                     KGAAYNILSQYPALLIILPAVLLTVAIIFLTRYRKKAKPMLLLSVAMIAGGGIGNLVD
                     RIVRGYVVDFFNIRIIPVFNIADIFICIGAGLFCIYVIFFDKREDERRKNL"
     gene            complement(12401..13018)
                     /locus_tag="PLM92_00525"
     CDS             complement(12401..13018)
                     /locus_tag="PLM92_00525"
                     /inference="COORDINATES: protein motif:HMM:NF016959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HPR23662.1"
                     /translation="MITPTEIQEKEFTRGVRGYKEEEVDMFLDLITLDLEKLVKENVR
                     LKAEVVALEDELDKSKGSEGAVLETLEAAKALMGDISVSAEKRAEILLKNAELDAELT
                     VREAREKAERLAQESMTLKNRYVDFRDKYERLLQAELERFENLSDDLFPEFDDERLQS
                     LIGEKESGSETVDVPKVKSSVSDEDFERDLASTASDRKTMVNIKR"
     gene            complement(13022..13492)
                     /gene="sepF"
                     /locus_tag="PLM92_00530"
     CDS             complement(13022..13492)
                     /gene="sepF"
                     /locus_tag="PLM92_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HPR23663.1"
                     /translation="MGVFSKFKDLVGIEEIDDDEFEEEIAESSAPLEKRPAETKSAYS
                     RRDPKEKVMPMQSRTTNPFKLVVIEPEGFEECPKLVDSLKTRKPIIINLEKIESETAR
                     KIFDFLSGATYALNGNVQKVANNIFVFAPENVDISSSIENKGFDFNNIHSDSWR"
     gene            complement(13503..14168)
                     /locus_tag="PLM92_00535"
     CDS             complement(13503..14168)
                     /locus_tag="PLM92_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HPR23664.1"
                     /translation="MEKIKENLSRIKADLDKGILLVAVTKTRTPEEINTAIDEGVTDI
                     GENKVQEIIEKYDRIKPVRWHMIGHLQTNKVKYIIDKVHMIHSVDSMKIAKEIDKRAA
                     AKGLKMDILIQVNPASEESKFGIGTDETEDLIKEILESCKNIRIRGLMCVVPFAEDPG
                     EVRGYFAQVRKQYDQYGKISHERLDFRYLSMGMTHDYKVAVEEGSNLVRIGTAIFGER
                     VYN"
     gene            complement(14170..15165)
                     /locus_tag="PLM92_00540"
     CDS             complement(14170..15165)
                     /locus_tag="PLM92_00540"
                     /inference="COORDINATES: protein motif:HMM:NF024112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HPR23665.1"
                     /translation="MPKPKKRTISVFILAAIALYIVIGVIPTLTGALTRTEILEYGDL
                     KARHEIKCWIIRSETVYSVASSGIVEYKSDEGTLLRKGAKVLEFTPVTEEKEEDARQE
                     SDQQKYIERLSGSMVPDSKFTAARKGIFTTMIDGYENFFTPEGMDDLKYSEVKGIGEP
                     AKNIAGDKVMAGEPIYKIADNSEWFLIFWVESGDIPNYKEGSTVKAELPAGSVSCSIK
                     NILQEGEKWRIIARSTGYYKDFVKNRMLEGYMVTTDTRGLLVSNSALTTKDDVTGVYV
                     KKATGEYEFVPVKTIATDGEKTLLAEDIFYDDNGEGVETVKVYDEVLKNPEADKN"
     gene            complement(15186..15923)
                     /gene="trmD"
                     /locus_tag="PLM92_00545"
     CDS             complement(15186..15923)
                     /gene="trmD"
                     /locus_tag="PLM92_00545"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HPR23666.1"
                     /translation="MNIEILTLFPEMFGPVRSSMLGKAIERGLLSLQTTDIRDFTQDK
                     HNKTDDYPFGGGAGMVMMPDPVFRALESLGAEKKRILYMSPKGSLLCKTKIEELAEEK
                     EIVIICGHYEGIDERIIEYWHAEEISIGDYILTGGEPAALVLIDAVARLIPGVLGNSD
                     SVKEESVYSGLLEHPQYTKPREYRDMKVPEILLGGNHKMIDLWKFEKALEITRERRPD
                     LFEEYIKDPGELTKEERKLLEKILDIV"
     gene            complement(15920..>16295)
                     /gene="rimM"
                     /locus_tag="PLM92_00550"
     CDS             complement(15920..>16295)
                     /gene="rimM"
                     /locus_tag="PLM92_00550"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HPR23667.1"
                     /translation="DRHEVKIENVRYKGKIVILKVENISDRNTAESQKGKDVHIWEDQ
                     TEKLPEGTYYVRDILGFSVFDTEGNQLGILRDVTGSGAQDILNIIDEEEKIIMIPAVP
                     EFLISADMEEKKIIVRLIEGMI"
BASE COUNT         3879 a         4013 c         2827 g         5576 t
ORIGIN      
        1 ctgttatttg atgcttcctc agtgaattcc tccagtgcct tattttcccc tgccactact
       61 gtctgcacgg gagaattgaa attcactgct tccaatatcc tgccttctga tatttgatct
      121 atgattccca gtattttttc tctgttaccg aaggctgcca ccatgccacc ctttggatct
      181 ccattgatgt cagtgcctgc ctcctgcata aaaacaccgc gttttttgac tatgtctata
      241 ccttttccta tttcatctat tgtgcctgcc actgtaagag ccgagtattc tccaagactg
      301 aaaccggcta tccccgtaag ctcaagggtt ttatcaagac cttcctttgc cgcttcctct
      361 ttaaaaaccc tgtatattgc cattgtgacc acatatatgg aaggctgagt cacataggtc
      421 tgccttagca tttcagctgt gccgttgaag caccattctt ttatctgctt tcctgccgca
      481 tcaaaaattt cccgggcagt tttgtgatta tcatatagat ccttgcccat ccccggatgc
      541 tgagctccct gtcctgaaaa tactatgcct atcttcatat atttctgctg cctccttgaa
      601 catttcggat atcatttctg cggccgactg ttctttgttg acaagaccgg ctatctggcc
      661 ggacataacc gaacccattt ccatatcgcc ggaaaacact gctcttctca aagaaccggt
      721 acagacttca tttatttctt cagcttccgc attgtttttt tccatttcca gaacctgtct
      781 tgccagtttg ttttttatca ctctgacagg gtgcccggtg cttcttcctg tggccactgt
      841 acttgagtcc ctggccttga gaatagcatc tttatagttg cgtgccacta tgcattcctc
      901 tgaagtcaaa aacctggttc ccacctgcac tccttcagca cccagcatat atgcggcagc
      961 tatgcccctg ccgtctccaa tgcctccggc ggcaatgaca ggtatcgaaa gagaatctgc
     1021 catttgaggt atcatagcca tcgtggttat ttcccctata tgccccccag cctcacagcc
     1081 ttcagctatc acagcatcgg ccccggctct ttcaacccgt tttgcaaggg tctgattggc
     1141 aacgacaggc aataccgcga tcccgttctc ttttaatctt tttatatatt tccccggatt
     1201 tccggctccg gtagtgacaa ccggcacttt ttcccgacat actatttcaa ttatttcttc
     1261 aacatttgga tcaagcaaca tgatgttcac tccaaaagga ttttctgtca ttgccctgat
     1321 ttttttgatc tcatttacta cccagctgcc ttgtgcacct cccgaggcta ttatgcccag
     1381 gccgccgccg cggcttactg cacccgccag actgctttct gctatcagcg ccattgctcc
     1441 ctggattatg ggatattctg tacccaggat ctgacaaact ctgttttgcc cagtcattat
     1501 catttgtcac ctcgcagttc tgttcttctg atcttgccac ttatggtctt tggcatttca
     1561 tcaagaaatt cgactttcct tggacatttg aaatgtgcca tatgctgcct gcagtattcg
     1621 attatctctt tttcaaggga tctgtctcct ttggttcctt ccacaaggac aatgctggcc
     1681 tttactatct gccctcttgt tttatcgggc agtcctgtgg ccgcacactc aagtacatat
     1741 gggagctcca taaggacaga ttccacctcg aagggaccga ctctgtaacc gcaggacttg
     1801 ataaggtcat cggttcttcc cacataccag aaatatccgt cttcatctct gaaggccgta
     1861 tctccggtgt gatatatgcc gtcatgccag cagtctcctg ttctttcttc gttgccgtaa
     1921 tatcctgtaa aaagcccaca ggggacactt ttatccgttc ttattactat ttcaccagtt
     1981 tctcctgtct ttgctgtatt ccctttctca tcaactatat ccacatcata agccgggctt
     2041 ggtttcccca tggatcccgg tctgggttcc gtccccagga gattacatac agtaagtgtt
     2101 gtctcagtct gtccgaaccc ttccattatc ttaagacccg tcttatcata gaactcctgc
     2161 cataccttgg ggttcaatgc ttcccctgct gtcgtcacat gtctgagttc cgaaagtctg
     2221 tatttcccaa gattcctttt tacgagtatc ctgtatgccg taggaggggc acagaaagtg
     2281 gttataccga atctggcaaa cagagacagg aaatcatggg gcacgaattt ctccatatca
     2341 taaatgaata ctgctgcctc gcacatccac tgtccgtata tcttgcccca caatgatttt
     2401 gcccagcccg tatcggacaa agtgaaatga agtccttcgg ggtccacatt ctgccagtat
     2461 ctggcggtga cataatgacc gagggggtat ttgaaactgt gtgtcacgat cttgggatac
     2521 cctgttgtac ctgatgtgaa aaacatggcc atggggtcat cgccccttgt ttctctcctt
     2581 tggaattctt cactgaagcc ttccatctct gcatcaaaac tatgccagcc ctcctttgcg
     2641 ggtcctgtca ttatcctgat gacatctctt tttccgccgg ctatggactt gtccgcctct
     2701 tccgtaacat catcttcatg agtgcagaat acggcattta tatctccggt atcaaatctg
     2761 aaattgaaat cttcttcctt gagctgatgg gtggccggaa taatgactgc tccaagctta
     2821 tgcagcgcta aaacagcaag ccagaattga taatgcctct tgagaacaag cattactctg
     2881 tctccctttt ttattcccag acaggaaaaa taatttgctg ttttatctga gagcacctta
     2941 agatccccga atgtgaagtt tctctcattt ttgtctttgt ccacataaag catggctctc
     3001 ttttggggtt ttatccgcgc caattcatca actatgtcat atgcgaaatt gaacttttca
     3061 ttgttggtga acgaaatact ctgcaaaaca ccgtttgcat cttcgcatac ggttatatac
     3121 tttttaatca aatcattcat aatacttaca tgctcttttc atctacatat tttacaagat
     3181 ccttaacgta ctgcatcttc tccgcatctt catctgaaat ttcgatttca aattcttctt
     3241 ctatgcgcat tattatttct actgcatcca gagaatccgc ctccaggtct ttcataaggt
     3301 gtgtttccat ttttactttt tcttcatctg cagaaagttg ctctgcgatt attgttttga
     3361 ttttatcgaa tgtcataata tacctcctgt tttataaact tctaactgat tataaaacag
     3421 agaagggaga aatgcaacat ttttatttga ttatcaaact attttgtttt ataagtaatt
     3481 ttcttttttt taaagaagaa aaaacggccc gaaagccgtt tttgtttgtc tgctgtatga
     3541 aatttttctt atcttttaat gtcgatttgc tgaaccggtg agatcatatc cttatatgat
     3601 ccttcctccg attattgtgc ttcttctgct tcatcaactg cttcctgcag ttcctctgcc
     3661 gagtcatact cttcaggatc caatcccagt tcatacgccc gttcaatcag caaagcatta
     3721 tcaccggagg catcatcaag ttcacctact atttccatgt ccagtgctga aaattcaccg
     3781 tcaccgtcaa catcaaaatc aaaaataccc attttatcaa ctcctgcaaa tatgaatact
     3841 gctgcatccg taattcattg gtcaatacaa actaatatct ttttatgata aaaacttcta
     3901 cttgttcatg tccatctgtt ttgccacttc ttcggcaaaa tcttcttcct tcttttctat
     3961 gccttctccc acttcaaaac gtacaaagga tgttacttcc atctctttgc cctttgcttt
     4021 ggcggtattt tctatatact gttttacagt tacctcgctg tctttgacaa acttctgctc
     4081 taaaaggcag tattccttaa gttccttttt cagcttgcct tccacgatcc tgtctaccat
     4141 ttccggcttt ttaccttcat tcagtacttg ttgtgtgagt atttttttct cttctgcaat
     4201 gaactccgga tccacatcgt ttccgtcaag aaatctcgga ttcatggatg ccacctgcat
     4261 ggccacatcc ttacccgctt cttctgtttc ttccccagaa gcttcggttt ttatccccac
     4321 gatgacaccg atggtgccgt taccgtgtat gtatccttta tatacggtat ctgcctcgca
     4381 aaacttctca aatcttctga tattcatatt ctcgccgatt tttgctatct tgcttttcag
     4441 cacttcctgt accgtggaac cttcaaaggg catatccatg aattcatctt tgcttttaac
     4501 gtcattttcc atcaccagtt ttgccagtga atctacaaaa tcaatgaatt cctggttttt
     4561 tgccacaaag tctgtttcgg agtttacttc tattattgca gctgttttcc cgtctttgga
     4621 gaatgcggtc cttaccagcc cctgagctgc tattctgtca gcctttttgg ctgccttggc
     4681 gatccccttt tctctgagca attccactgc cttttctata tctccatctg tctccatcag
     4741 gacattcttg cagtccatca tacctgcgcc tgttatctca cgtagttcct ttaccatgct
     4801 tgccgttact gacatttatt ttccctcgct ttcttcagaa acatcctctt cactctctga
     4861 tgcctcttcc gttttttctc ctgtcgcttc tatttctgcg gtttctgctt cttctgtctc
     4921 ttcttcgtag ccttcgtcaa agctttctcc ctgatttgat tctattatgg catctgccat
     4981 acttgacgct ataagtttta ctgctcttat ggcatcatca tttgccggta ttacgaaatc
     5041 cacatcatcg ggatcgcagt tggtgtcgac tatccctatc accggtatcc caagtatcct
     5101 tgcttctctt acggcatttg cctctttctt ggggtcgacc acaaaaagtg ctcccggcat
     5161 gcctttcatg tctttgatgc ctccgtagaa tctttcaagt ttttccgctt cattccttaa
     5221 tgtaagcact tcttttttgg atagtttttg gaagattccg tcttcttcca ttttttggat
     5281 gtcaaacagc ctctttattc ttgaagaaat ggttttgtaa tttgttaaag ttccgccctt
     5341 ccaactctga ttgacataga acataccgca tcttaaggct tcatcctgta tggccgcctg
     5401 ggcctgtttt tttgtgccga caaaaagaac cggtttaccg ctttcgccta cggttctcat
     5461 gaattcgtaa gcttcttcga ttttcttaac tgttttttga agatctatga tatatattcc
     5521 gtttctctca gtaaatatat acggagccat tttggggttc catcttctgg tctgatgtcc
     5581 gaaatgtaca cctgcttcaa gtaattgttt cattgaaata actgacattt tattttcctc
     5641 ccggttatac ctccatcctg gcatccccca aaagaaaccg catatgcggc accgtctttc
     5701 ggcagccgga tgtgtgaatt ttacgatgac gctctcttag cgacaccgca aatcagtata
     5761 ctataatatt tcagtatttg caagggattt cttccgcatg ggcctttatc agatatgtgt
     5821 tgaagccctt ctttgttttc gagtatgcag ctctgcccag aaaaacgggt tctttgctgt
     5881 caaaaaaata tattgctcca tcagcgatcc ttcggacatt atccagattg accgcaaggt
     5941 atgtatggca tctgtggaaa catcctgaaa gcacgggcca gcattctttc attttgccat
     6001 aatatgtaaa agtttctttt gcggtgacga ttatgatctt tctttctctc tgctctatga
     6061 atagtatctc attctgcggt attctggcag tttccttttt cttgatgaca actattcgtt
     6121 ttttccgcat ttcatattgc attatgtcat cgtttttcat cttgtccctt cccttttgaa
     6181 tttcccccgg atcctcagaa catcaattcg atatgcaata taaaatgcgc cgaaaggcgc
     6241 attataaatt tttttattcc tgacgcttat atgtgatata atcctccgct atttcgtttg
     6301 cggccaccga caccggtctt tcttcggaaa aatcgcgacc ttcttcatcg gtcccttcaa
     6361 taagtatagg tttaccgtcg attatgtctc tggctctttt agcagcaagt ataacaagag
     6421 tatacctgct gtctgctttc tttcttatct cattaataga tggatacagc attatatttc
     6481 ctccttatat ttttctataa gctcataaat gtcttctgaa actcttgaat gttctgcttt
     6541 tattattgct ttcaccctgc atacagcttc ttcaagttct ccgtttacaa cacagtaatc
     6601 atacttgtct atatatgcga tctctttcat cgctccggcc atcctgactt ctatgctttc
     6661 gtttttttcc gttccccttc cggtgatcct ttctctgagt tctgccatgg aaggcggcag
     6721 aatgaaaata aaaactccgt acggatacac tttttttatg ctcatggctc cctgtatatc
     6781 tatatcaaga accacatcat tgcccgaatc cagtttctcc attaccatct gtttgggggt
     6841 accgtaataa tcctggtaaa catacgccga ctccagaaat ccgtcaccgg ctttcatttc
     6901 aaggaatttt ttttctgaaa caaaataata atccctgccc tcgatttctc cttcacgggg
     6961 ctttcgtgta gtcatagaaa cagagaaaaa aagtttttct gtttcttcaa gtaatcttgt
     7021 acatatcgtt ccttttcccg ctcccgaagg gccggaaatg ataaacaatc gtcctcttga
     7081 catttttgct cctccgcatc attgatgcga atatttatta tatcatcaca gtgctcctca
     7141 ttcaacataa aattatatat gctgttattc tatattttgg acctgttccc ttattttttc
     7201 gacttcgctc tttatttcca gcattatacc ggttatttcc atgtcatttg cctttgatcc
     7261 gattgtattg gcttctctgt tcatttcctg cacaagaaaa tccagtttct tgccgtcgga
     7321 tctgtcactc ttgcttatta tgtcgtccat ctggatcata tgactgtcaa gtcttacaag
     7381 ttcctctgtt atatttgcct tgtccgcaaa tattgccgcc tccagcagta tcctgtcttt
     7441 cgggatctct atttctttac ctataagctc ttctattcgc tccgccagtt ttatcgcata
     7501 tgttttgctt acctggggag ctttcttctc gatctttttc agatcatctc tcacctttgc
     7561 tgctctcatt gccagatcct ctgccagttt tgccccttct ttggtacgca tggaatcaaa
     7621 agcatcaaca gcatccctta ctgcgatctc aacagatctc aatatctcct cttcatcctc
     7681 tacatcaggt atagtcttca tcacttccgg cattccagat ataagagcca gatctatgct
     7741 gcccggcaga ccaaattctt ctttaagttt attgaggttc ttgtgatact gggaagctgc
     7801 tgctgtattg aggcttatct tgagatcatc ttctgtgacc gcctctataa gtatggctac
     7861 ttcaactttg ccccgtttta cggtctcctt tacgatattc ttgattttat cttctgcaaa
     7921 attatacctt ctgggcattt tcacattaat gtcactgtat ctgtgattta cggaacgcac
     7981 ctctgcagtt atgatccttt tgccgtcact atactcccct ttcccaaatc ctgtcatact
     8041 ctttatcata caattttctc cttttcaata ataatttatg ataatatatt attatatcac
     8101 agtactatcc ggaaagtgag atttttatgg cttttgacgg catttttacc ggagcggcgg
     8161 cctttgagct gaatgaaaaa ctccgcggtg ccaaagtaga aaaaatatat cagcccgaaa
     8221 gagacgaact ggtttttcat cttcactgcc cctcagggga tcacagacta tatgcttcct
     8281 gcagcagcaa ccatccggga gtatatctga cttcggaaaa attccagaat cccgcttctc
     8341 ccggttcttt ttgtatgctt cttagaaagc atatcggcaa aagccatata agcgatatcc
     8401 atcagaagga ctcagaaagg atcattgaga tcctttttac gggtcgggat gaaatgggtt
     8461 atggtaaaaa caaaaaaata atattcgaaa taatgggtaa acacagtaat ataatacttg
     8521 tgaatgccga tgataatatt attacagaca gcatcaaacg tatctccctg ggcatgagcc
     8581 gaatacgtcc cgtcctcccg ggaagaccct atgaataccc gcctgcacaa gacaagatct
     8641 cccttttttc agtcactttg tccgatgtca atgagctctg ccgtgaaaac gacataagcc
     8701 gggctctgct ttctcgcata cagggaattt ctccccttat atcagacgaa ctctcaatgg
     8761 aaaaaagctc tgatgccgtc ttcaaaaaaa tctgcatgat aagggaaagt gtaaaaaacg
     8821 gaaactttgc ttctgcagga tggctgaaag aaaacggaga gccgcgggac ttccatgtga
     8881 tccctgtttc tgcctatgag gaacttgata tcatacgttt cgaaaaccct agcgaggctg
     8941 tctcctggta ttataaaaac aaggactctg ccaacaggat aaggcagaaa tcatcagatc
     9001 tcaacaaaat catttctgcc gcattgaaga aactgagact aaaaaaacaa cgtcttcgtg
     9061 aagatttact atatgccgaa gagtccaaaa aattccgtct ctacggcgag cttatcactg
     9121 cgaatataca ccggataaaa caaggtgaaa attctgccgt ggtttttgac tataataatg
     9181 gagaagagat cactatcccc cttgacgtaa gactttccgc ctctcaaaac gcacaaatat
     9241 attttaaaaa gttcagaaaa tcaaaaacag ctttggttga aaaacagaaa cagctcagtg
     9301 aaacagattc agatataacc tatctggaat ccactgccga acatcttaac cggagtgaaa
     9361 aagtatctga catcgatgcc atccgtgatg agcttgtcga aaccggtttt atcaggaaaa
     9421 gaagggatct gcccgcaaaa aaaataaagg ccgagccttt ttcatatgtt tcttcggagg
     9481 gatacgtgat ccttgcaggc aggaacaaca gggaaaacga ttttcttaca ttcagaaaag
     9541 catcccgcac ggatctgtgg ttccatacaa aagatattcc gggttcccat gtgataatgc
     9601 ttacagaggg cagggagccg ggagaaaatt ctatacttga agctgctgct ttagctgcat
     9661 accacagcaa aggtgcatct tcggaaaatg tgcccgtaga ctatactcaa atacgctatg
     9721 tcaaaaaacc ctccggtgca aagcccggca tgtgtatttt cacaaacaat aaaacactgt
     9781 atgtcaagcc ttcagtcccc gtatccattc tatcatcttc atgatcacct cggtgccgtc
     9841 gttctctttg tttccattgt gtatttcatg aaacatgcct tcccactcta tatatgtaca
     9901 ggcttctttt tcgtgtgata cgaacttgcg gcttccttcg atactgcata cccgatcctc
     9961 agtgccatgc ataaccaaaa ggggttgtcc ctttgatctg ccattgtctt cattttcgtt
    10021 ttttgccaga gcttctccct tttcaaaagc ttcaaatgca caaagcattg atattttatc
    10081 atgcaccata ggatttgttt tgtaaggttg aacagaaagc ggatgcccca gatcttcttc
    10141 gtttactgat gattttattg tcatttccgg gcatattcgg gccatgattt tcacaaacag
    10201 aaaaagcgga gccgccggct tgttcaccag ttctatccat ggagccgaga ttatatatgc
    10261 aaagggttta tcattcaact ttcctcttgc tctgtagtca agggcgatat tgcctcccaa
    10321 actgtgtccg taaagtatca ccggtttatt ggggtacttt ttcattgcat gttctaaaag
    10381 aatatctatg tctccaagca ctgattccct tggagcacaa tgacctcttt tgcccgaaga
    10441 tgccccgtgt cccttaagat ccatagaaag gcaggctata ttggcctctg aaagaaattt
    10501 gttcatacgg tcataacggc ccgcatgttc tcctatgccg tgaatgatgc aaataacata
    10561 gtcgggttct tctttcgccc ataaatatcc ggttattctg tctttttctc cggaaccttt
    10621 gatcggaaaa ttttcaaaca tatttttatc ctttccgtta tacttcattt caagcccttt
    10681 atattacatt ttgtgctctt gttttgcctg gccattgttt ttaatttttt tctgtatata
    10741 ctcgtattaa accctaaaat tgtttgagac cgcaacgaaa aaggaggcag aaatgaataa
    10801 cagatgttcg atgtgtgata agttttgtaa aaccaggcct ttcagaggcg gttatatatg
    10861 cgaggattgt cttgatattt tcaagtcacc gaatgccaga ttttggagca tcgatacata
    10921 agttgaaaaa cacaaaaaca ggtctatccc acgctccata ctttccccta tcttaccaat
    10981 ttcaattgct gcggtttcag cagggtcgtt ttataaacgg ccctgtatta tttttcattt
    11041 cttatctttt ccaggacggt ttcgaattca ttgggcaaag gacttttaaa ttcaatgtat
    11101 tccgccctcg acggatgcat gaatcccagc aaatatgcat gaagcatctg tccttcgatc
    11161 ccgtagcgat tctttttcgg tccgtatata tagtcaccga aaagtgggtg ttttatatac
    11221 gccatatgta ctctgatctg atgtgttctt cccgtttcaa gtcttgcttc gatcagggta
    11281 tattttccga atctttcaat caccttatag ttggtcactg cttccctggc attcaaacct
    11341 cccacagtct gtctgaggcg gtttttcggg tctcttccca aaggttcttc tattctgccc
    11401 tcatcttcaa caatattgct gtaaaccaaa gctttatatc ccctggttac gctgtgctct
    11461 gccagctgcc gggcaagaga cctgtgggcg aaatcgtttt ttgcgatcat tatcagtccg
    11521 gtagtgtcct tgtctatacg atgcactatc ccgggtcttg ctatgccgtt gatacccgaa
    11581 agactactgc cacagtgaaa aagcacggca ttcaccaatg tatgggaata actccccgca
    11641 gcgggatgga ccaccatccc cttttgtttg tcaataacca acaaatcatc atcctcataa
    11701 actatatcaa gaggtatatc ttcgggtaca atatttgtct cttccgattc cggaagaagt
    11761 atccttattt tctctccggc ttttacctta tgcttttttg aaaagcagac ttcccctccc
    11821 agtgaaacca tgcctttctc gattaatttc tgtatgtgat ttctcgatgt ttccggcaaa
    11881 gaaaaagaaa gaaccttgtc aagtctggtt ccgtctgttt cctggtccgc tgttatgtta
    11941 aagatttttt cttctttcat cttccctttt atcaaaaaat atcacatata tacaaaacag
    12001 tcctgcccct atgcaaatga aaatatcagc gatgttaaaa accgggatta ttcttatgtt
    12061 aaaaaaatct acgacatatc cccgtactat cctgtccaca agattaccga tccccccgcc
    12121 tgctatcatt gccactgaca gcaacagcat gggtttcgct ttttttctgt atctggtaag
    12181 gaatataatt gctaccgtca aaagcacagc cggaagtatt atcagaagtg ccgggtactg
    12241 ggaaagaata ttgtaagccg cacctttatt ttctacataa gtaagataaa gcacaccatc
    12301 gagcagtgtc acgggatgtg ccgggtcaag tccggaatag gccagtgcct tgatcatctg
    12361 atccaaaaga acgattgctg ctattatcaa ataatacata tcatcttttg atgttgacca
    12421 ttgtttttct atcggaagcc gtgctggcaa gatctctttc gaaatcttca tccgaaacgg
    12481 aactttttac ctttgggaca tccacggtct ctgatccgga ttctttttcg cctataaggc
    12541 tctgaagtct ttcatcatca aattcaggga acaaatcatc acttagattt tcaaatcttt
    12601 ccagctcagc ttgaagcagt ctttcgtatt tatctctgaa atcaacgtac ctgtttttga
    12661 gtgtcatact ttcctgggcc agtctttcgg ctttttctct tgcttctcta actgtcagtt
    12721 ccgcatctag ttcggcattt ttaagaagta tctctgcacg cttttctgcg cttacagata
    12781 tatctcccat aagtgctttt gcggcttcca gagtttccag aacagcccct tcggaacctt
    12841 tgcttttgtc cagttcatcc tcaagggcta cgacttcggc tttcaggcgc acattctctt
    12901 tgacaagttt ttcaagatcc agagttataa gatccaaaaa catgtctact tcttcctctt
    12961 tgtaacccct tactcctctt gtaaattctt tttcctgtat ctctgtgggt gttatcattt
    13021 tttacctcca agaatcactg tgtatattgt tgaaatcaaa tcccttgttt tctatgctcg
    13081 aggaaatatc cacattctca ggtgcgaata caaaaatatt gttagcgact ttttgaacat
    13141 ttccgttaag cgcatatgtg gctccgctaa gaaaatcaaa tatcttcctg gctgtctcgg
    13201 attcgatttt ttcaaggttt attattatgg gttttcttgt tttcagagaa tccacaagtt
    13261 tggggcactc ttcaaatcct tcaggttcta tgactaccag tttgaaagga tttgtcgttc
    13321 ttgactgcat aggcatcact ttctcttttg gatctcttct gctgtaggcc gattttgttt
    13381 cagccggtct tttctcgaga ggggcactgc tctctgcgat ctcttcttca aattcatcat
    13441 catcgatctc ttctatgccc accagatcct tgaacttact aaatactccc atattatcct
    13501 ccttaattgt aaactctttc tccgaatatg gctgttccta ttcttacaag attggatcct
    13561 tcctccactg caaccttata atcatgggtc atgcccatag ataaatatct gaaatccagt
    13621 ctttcatgac tgatttttcc gtactgatca tattgtttcc tgacttgcgc aaaatatccc
    13681 cttacttctc ccgggtcttc tgcaaagggc actacacaca tgagccccct gatccttata
    13741 ttcttgcagc tttcaagtat ttcttttatc agatcctctg tttcatcggt gcctataccg
    13801 aatttgcttt cttcggaagc agggttcacc tgtatcagaa tatccatttt gagtcctttt
    13861 gcagcagctc ttttatcaat ttcttttgca attttcattg agtcaacaga atgtatcata
    13921 tggactttgt caattatata tttgacctta ttggtctgca aatgacctat catatgccat
    13981 cttaccggct ttatccggtc atacttttca attatttcct gaactttatt ttctccgata
    14041 tccgtaactc cttcatcaat ggctgtattg atctcttcag gtgttctggt ttttgtaact
    14101 gccacaagca gtattccttt gtcaaggtct gctttgattc tactaagatt ctcttttatt
    14161 ttctccatat cagtttttgt ctgcttccgg gttttttaga acttcgtcat aaaccttcac
    14221 agtttctact ccttctccat tgtcatcata aaatatatct tctgccagta atgttttttc
    14281 tccgtctgtt gctatggttt ttacaggaac aaattcatat tctcccgtag ctttttttac
    14341 atatacacca gtaacgtcgt cctttgttgt aagagcactg ttgcttacca gaagtcctct
    14401 tgtatcggtg gtcaccatat atccttccag catcctattc tttacaaaat ctttgtaata
    14461 acctgtgctc ctggctatta tcctccattt ttctccctcc tgcaggatgt tttttatgct
    14521 acaggaaaca cttccggcgg gaagttccgc ctttactgtg ctgccttcct tgtaatttgg
    14581 tatatctcct gattcaaccc agaaaataag aaaccattca ctgttgtcgg ctatcttgta
    14641 aatgggctcg ccggccatca ctttgtctcc ggcaatattt tttgcgggct cgcctattcc
    14701 tttaacttct gaatatttta gatcgtccat accctcggga gtgaagaagt tttcataacc
    14761 gtctatcatc gtggtgaata tgccttttct tgctgcggtg aacttgctgt caggtaccat
    14821 tgaaccggaa agtctctcga tatatttttg ttgatctgac tcctgccggg catcttcttc
    14881 tttttcttcg gtgaccggag taaattcaag tacctttgct cctttcctga gaagagttcc
    14941 ttcatcggat ttgtattcta cgatacctga agaagctacg gaatacactg tctcgcttct
    15001 tatgatccag cacttaattt catgtctggc cttcaggtcg ccgtactcaa gtatttctgt
    15061 tctggtcagc gctcctgtca gtgtcgggat cacacctata acaatatata gtgctattgc
    15121 tgccagtatg aatacagata tagttctttt ttttggtttt ggcatgttat gtctccgtct
    15181 acattttaca ctatatccag tattttttcc aataattttc tctcttcttt tgtaagctct
    15241 ccgggatcct ttatatattc ctcaaagagg tctggtcttc tttctctggt gatctcaagg
    15301 gccttttcaa atttccatag atctatcatt ttatggttgc ctccaagcag tatctcaggg
    15361 actttcatgt ctctgtattc tctcggtttt gtgtattgag ggtgttccaa aaggccggaa
    15421 taaacagact cctcttttac cgaatcactg tttcccaaaa ctccgggaat aagtctggca
    15481 acggcatcta tgagcaccag ggcagcgggt tctcctcctg tcaagatgta atcgcctatg
    15541 gatatttctt ctgcatgcca gtattctatt attctttcat ctataccttc gtaatgaccg
    15601 caaattatga cgatttcttt ttcttctgcc agttcctcaa ttttcgtttt gcacaaaaga
    15661 ctgcctttag gagacatgta aagtattctt tttttttcgg cacccaaaga ttcaagtgct
    15721 ctgaaaaccg gatccggcat cattaccatt ccggctccgc caccaaaagg ataatcatcc
    15781 gttttattat gtttatcctg ggtaaagtcc cttatgtcag ttgtctgaag gcttaggagg
    15841 cctctctcaa tggctttgcc aagcatactg cttcttacag ggccgaacat ttccggaaaa
    15901 agcgttagta tctctatatt catatcattc cctctatcag cctgacgatt attttctttt
    15961 cttccatatc tgcactgatc aaaaactcgg gcacagcagg gatcataata atcttttctt
    16021 cttcatcgat tatatttaaa atgtcctgtg ctccgcttcc ggtcacgtct cttaatatac
    16081 caagctgatt cccttcagta tcaaaaacag aaaaacccaa aatatcccgg acataatatg
    16141 tcccttcggg aagtttttcc gtttgatcct cccaaatatg aacatctttg cctttctgac
    16201 tttccgctgt gttcctgtcg cttatgtttt caactttaag tattacgatt tttcctttat
    16261 agcggacatt ttctatttta acttcatgcc ggtct
//
LOCUS       DAONHK010000006        16124 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_2842_length_16124_cov_5.173004, whole genome shotgun sequence.
ACCESSION   DAONHK010000006 DAONHK010000000
VERSION     DAONHK010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 16124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16124
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2842_length_16124_cov_5.173004"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="PLM92_00555"
     CDS             <1..402
                     /locus_tag="PLM92_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPR23668.1"
                     /translation="FDGRGPDLKSLLNNAFGMDEGSDYLESLADNKVIKFVAGTDSYM
                     VRMTVKQLKQTRYYYPNGPDGAEIRDTAKGSRGSEASRQGAIPVPVRIHLGETGAEGG
                     ETVLRVGQVAPNEQNWPLAVKDIARGQTADK"
     gene            525..1163
                     /locus_tag="PLM92_00560"
     CDS             525..1163
                     /locus_tag="PLM92_00560"
                     /inference="COORDINATES: protein motif:HMM:NF024702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat protein"
                     /protein_id="HPR23669.1"
                     /translation="MYYTTNGILPTMDSDIYNYNTYGTLSSRTITVPATVGGTFTVKA
                     FSMKYGKLDSDVQTFSYKIISLPKQGGTYTVGSSKYKITGSAASGGRAAYMRRIKKTY
                     KISSVPASAKINGYTFKITSVADKAFRKNKKLKKVTIGSNVTKIGKQAFYGDKKLKTI
                     VIKSKKITKCGKAAFKGINKKAKIKVPKSKLKKYRKLFKNKGQAKTVRIVKL"
     gene            1285..1746
                     /locus_tag="PLM92_00565"
     CDS             1285..1746
                     /locus_tag="PLM92_00565"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HPR23670.1"
                     /translation="MGNDMFGGLMKGLSSVLPQDDPNIKKFTAQSKINDLKEEETGIY
                     AELGKKIIEEEPGKYPGFEERLRAIRIELNEAESGAKKADMEAKEAEKVKEAENAKRT
                     CPNCGTINEEGVKFCSECGTEIGQGLTCPGCGAALSPGTKFCGSCGAKVGE"
     gene            1749..2123
                     /locus_tag="PLM92_00570"
     CDS             1749..2123
                     /locus_tag="PLM92_00570"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HPR23671.1"
                     /translation="MATYKQPCIHCGTFIERSSSFCPKCGSNSPFGYSCPSCGQSIEK
                     EQKRCSSCGRDLYVSCPHCGELTFVQEKCEVCGKSLMKICGNRRCKAEQFFDLVKCTA
                     CGKSLIPETEKKGFFNRKKQDD"
     gene            2144..2899
                     /locus_tag="PLM92_00575"
     CDS             2144..2899
                     /locus_tag="PLM92_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015412916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HPR23672.1"
                     /translation="MMKAFTRNYDDNSTEAGFQFTFYCDICGDGYKSSFIESETYDKK
                     QKIQGVARGAGILGSLMGGVGYNTGWAAERAGDVMAERFEERSPEWRKEYENAFNRAQ
                     NEAQQHFHRCHACQKWVCDADWNEDEGMCVECAPRTEIYTAKAKADAIKRNIDEKAAE
                     ATIWDGDLKSTTTVCPSCGKPAGNGKFCNNCGASLELNKCPSCGAENAQGVRFCNQCG
                     AKMDGPKVTKCPGCGKEMEPGAKFCGECGTKIE"
     gene            complement(2934..4022)
                     /locus_tag="PLM92_00580"
     CDS             complement(2934..4022)
                     /locus_tag="PLM92_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019243535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2804 domain-containing protein"
                     /protein_id="HPR23673.1"
                     /translation="MKNSFINENTGQKEITKKQNLLTPEGHLSAPGYSRRHLQVYNRS
                     HIKASGRRIKEWDYYYIGNEKYGIALTVADNSYMGLDSISLLDFLRPWQHTKSPMQPF
                     TFGKKKMPSSTLEGNVKTKDKNYFICFEHRSSIRHLSFYMDDFYKGKPIEGDLTLKCP
                     DMDSMIIATPFNEDDLAFYYNEKINCMPASGIVKFDGRDHIFDPSDSFGVLDWGRGVW
                     TYKNTWFWGSASGLHEGSPFGFNIGYGFGDTSAATENMIIYKNKGHKISRVKFNIPDK
                     PGMKQSDHKMGGEDFLSPWSFTSDDGRFEMDFIPVIDRSSRTDLGIICSNQHQVFGSF
                     SGKAVLDDGTLLKIKNLPGFAEKVFNKW"
     gene            complement(4044..5222)
                     /locus_tag="PLM92_00585"
     CDS             complement(4044..5222)
                     /locus_tag="PLM92_00585"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HPR23674.1"
                     /translation="MGKFLIRKFIKNYEDVKDPKVRQNYGKFAGIVGIISNLLLCVMK
                     ILIGLISSSIAIIADGVNNLTDASSSIITLVGFKMASQPEDKDHPYGHARIEYLTGLF
                     ISILIIIVGLFLLRSSVSKIIDPKILSFDYITITVLILAILIKVWQTFFNMYVGKKIN
                     SMTLIATGTDSRNDVIATSAVLIGVIITKFTGLQLDGWLGSLVALFIIWSGITLIKET
                     SSPLLGEAPDEDMVSEIEKMASAPGGVIGIHDLVVHNYGPGKVFASIHIEVDADEDIM
                     KSHDMVDNIEREIGETLGIHCVAHMDPIKRNDPAVKKVEPVITETIKSFPGVTGMHDL
                     RVVPGPTHTNIIFDLVLDHECKTEEKEIKGKVQDELQKIDPCYFVVITFDRSYTQNSI
                     "
     gene            complement(5228..5488)
                     /locus_tag="PLM92_00590"
     CDS             complement(5228..5488)
                     /locus_tag="PLM92_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006779382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HPR23675.1"
                     /translation="MKHTYIPRGVCPTKLEFDLEGDVVKNINFFGGCNGNLKAMSSLL
                     DGMTVKEITEKLNGNTCGMKSTSCADQLTIALNKALSENREK"
     gene            5558..6115
                     /locus_tag="PLM92_00595"
     CDS             5558..6115
                     /locus_tag="PLM92_00595"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HPR23676.1"
                     /translation="MKVVCAGNSIVNGFPHRRSCSFPSVLREMTGWKVINKGINGETT
                     VMLAERFDRDVLLHEPEKVIILTGTNDFIFETETPEGAMKNIARMAEKAKEKGVEVVL
                     LTPINCNETQAAECWMKGSGTDYHEVNRKLSKLAEMIRDHGSMNCCKVVDLEVEYKKF
                     AKFHDGIHPTVEGQAYIAQKVKEGI"
     gene            6117..7157
                     /locus_tag="PLM92_00600"
     CDS             6117..7157
                     /locus_tag="PLM92_00600"
                     /inference="COORDINATES: protein motif:HMM:NF022702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HPR23677.1"
                     /translation="MRSDVTKKKKSKAPIIILVIILIAALGAGAYFLLSKDNKKSGGI
                     ISDEIINPLTGESVEELLPRPIQFSIDNAAPARPQSGLSAADIVFEYPVEGSITRIQA
                     IYYTKLPETVGPVRSARPYFVDTAREYKAVFVNHGWSPEAKEYLESGVVPYISGLTYS
                     GGVFYRADDRPAPDNSMIHTDKLLEAIEKEGYDEEQDVRTFNFLEKGQKVKGQKATEI
                     NINYLTTKCSYEYDREKKLYKKFSNGEVYIDKETGDQITAANVMIQFVKSQVLDNKGR
                     LGIDMKAGGDAMLFRKGKVIKGTWSREDMDSPTIFTAANGKEMKMATGNTWIQVLDQN
                     HKIEYNSDEEEQ"
     gene            complement(7216..8580)
                     /locus_tag="PLM92_00605"
     CDS             complement(7216..8580)
                     /locus_tag="PLM92_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006574202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HPR23678.1"
                     /translation="MKKLERRALMCLLLAGMLAAGLCFYVGKWLVYGSEWVSYPANKH
                     VFTDGKINTGSIYDRNGQVLLENTESGEPEYSDSYSVRTATLHAVGDIDGNILTGANR
                     AFAGKLVGYNPVTGTYSAGGEGRQIYLTIDADACVTASEALGDHKGAVMVYNYKTGDV
                     LCMTSSPNYDPTDPPEIKEDDTSGVYLNRCTTARIVPGSIFKLVTSAAAIEKKKDLES
                     WTYNCTGKKQFESSEVDRITCPYPHGEVDFEEALAVSCNCGFADLTLSLGIDNIKKYT
                     KKAGLTDSYNINGIHTTPGSFDFPSEDVNIAWTGIGQYHDLVNPCSMMIYAGAIANGG
                     KAAVPNLIDSIKTNSGLPAGINWSRKTDTLIESETASVLSDMMHNNVIETYGEYNFPD
                     LDICAKSGTAEVGGGNSPNAWFTGFLRDEKNPYAFIVLVENGGSGANVAGSVANQVLQ
                     ELVN"
     gene            complement(8577..10349)
                     /locus_tag="PLM92_00610"
     CDS             complement(8577..10349)
                     /locus_tag="PLM92_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009643296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HPR23679.1"
                     /translation="MELFTSLSEASKPFMEKISDVFQSDPTFAVYFTTAARWIFVILA
                     VFILFKSIRSLLEAKNTPETWAFLALPDGSHVPLTHWENVIGRAKSADVVVDILTVSR
                     NHGTLCRDEDGNWRYNDLGSKGGSMINEKHVYRSTPVKAGDTISLAGVNCVLVPASLQ
                     EKMEDLGRRKKRSRKFPPWTLLLSLTIFQILLCLQLIVAEGGELPITVPLSFAGLTVL
                     MWAFCTFLKVMRSTNFEMEVIAFFLCTLNLAITATAAPNEVSKQLITIILGVVLFFCL
                     CIYLRDLSRTLLIRRILVAASVLLLLINLAFGTLTYGAANWVSIGGFSFQPSEFVKIA
                     FIFIGSATLDELYEKKNLGLFMLFSLFCLGCLAVMGDFGTAAIFFVTYLVISFLRSGE
                     FSKLILTAGGAFAAGLMVLRFRPYIAERFATWGHAWQYADTGGFQQTRTMSAGASGGL
                     VGYGSGDGWLHNVFASDTDLVFGILCEEWGLIIAILAVLAIITLGVFAVRSIMGGRST
                     FYTIAACGATSLFIFQTMLNVFGAVDIFPLTGVTFPFVSNGGSSMLVSWGLLAFLKAA
                     DTRQGASIAVRKIEDEEEIEGGTL"
     gene            10457..11335
                     /locus_tag="PLM92_00615"
     CDS             10457..11335
                     /locus_tag="PLM92_00615"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HPR23680.1"
                     /translation="MTKTKKRWGDRKDGTRLREIDGLHQTMAHLLPNRCDAEIFIKET
                     MDVTNIIRYIEEKKAEDPASRLKTFHIFVAAIAKTVYHRELLNRFVAGRRFYQRNVVS
                     LAFVVRRSFNDESEESLLVLKANGETELNDISRKITGDVKEIKEGEKGDIDKVLDFFA
                     RLPRPMQMLVVKLARILDFFGKMPEAITSGDTNYASVLLSNIGSIKCGAPYHHLNNYG
                     TNSIMITIGEIHKEQVPDKDGVPVIRDVVDFGITLDERIADGFYFARSVRLLKHIIEN
                     PVLLEEPLREVVEYEG"
     gene            11325..13097
                     /locus_tag="PLM92_00620"
     CDS             11325..13097
                     /locus_tag="PLM92_00620"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HPR23681.1"
                     /translation="MKVKTPWYSSYGNVPSHLDYPGCSMIEYIEKNAKEYPQLTAYEF
                     LGTSVTYEEFLMQVHQCAKALRATGIEKGDRVTICMPNTPQGVIMFYAVNLIGAVSNM
                     VHPLSAEGEIEFFINISKSKAILVLDAFYQKVRNIREKTPSLEHVIVASIATELPPIT
                     KIGFKLTKGRKIPRIPAGDDFVSWTDFMSSGERYIEEYIAHSRADDPASILYSGGTSG
                     KTKGIVLSNLNFNALAMQTGAASETIAPGQKMLAIMPMFHGFGLGIGIHTMLQGGCCC
                     HLVPQFSVETYAKLLRKVQPNYIAGVPTIYEALLRNENMKGVDLACLLGVFSGGDSLS
                     IELKRKVDNFLKEHGATIQIREGYGLTECVTASCLTPKDIYKEGSIGIPFPDTYYKIV
                     KASTKNEVPFGEEGEICVSGPSVMMEYLEDESETESTLQRHEDGLLWLHTGDLGMMDE
                     EGFVYFKQRLKRLIISSGYSIYPSQLENIIDANENVLMSTVIGVPDPYKMQKVKAFVV
                     LKKGIKETEEIKEEIYQHCLKNIARYAMPYEFEYREKLPQTLVGKVAYTVLEMEEHAK
                     LRQMQDKSKKKDAKDNKGSNKTED"
     gene            complement(13208..15589)
                     /locus_tag="PLM92_00625"
     CDS             complement(13208..15589)
                     /locus_tag="PLM92_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008369245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HPR23682.1"
                     /translation="MGIKELFGKELLFFDGGLGTMLQAAGLKGGRSPELWNLEEPQVL
                     LDIHEKFLDAGCNILTSNTFRARDAKYMAAGVKLAREAVKKYNAKTAGDPDRIQGKEK
                     VFVAAELGPSGGLLEPLGELSFDDAYKVFADLVHAGADEGADAIIIETMPDIYDMKAA
                     ILAAKENCSLPVIASMTFEQNKKYLTGGDVKTAVAVMEGLGADVIGTNCGFGPAQIKN
                     IVKEFFKYASVPVMANPNAGLPRSINGETVFTMGPEEYSKEAVSIAESGAWLLGGCCG
                     TTPEHIRREIELCRNVVPPAVTKKHETFAASYAQAVRIGSRPGDKTVNRHKKAGEKIN
                     PTGNKLVKEALKAGDTATVKELAAKQQQAGADILDINVGLPEIDEKTMLPLLIKEVQS
                     IVTLPLIIDTSDIEAMDEALRIYNGKAVINSVNGKKDSMEKVFPLVKKYGGVVIGLTL
                     DETGIPKTPEGRLVIAKKIIETAAEYGIDKKDVIIDPLAMTISVDENAGETALAAMEL
                     IKKELGTATVLGVSNISFGLPDRDSVNSAFYREAVSRGLDMGIIDPFSEKMMAAADGS
                     RDARDSYKCSLENMKNKTGDASTLKEAIVEGLKEKASALTRELLKNKLPLDIIENQIV
                     PALNKVGRGFEDGSLFLPQLLMSSDAAQSSFAVLKSAMKSDVQKKKDRVILATVRGDI
                     HDIGKNIVKVLLENYGYEVVDLGRDVPPEKIAEAAEKEKINIIGLSALMTTTAPSMKD
                     TIDLFRDRGLYDECRFIVGGAVLSESYAKEIGAHFYARDAMATVHYVQKLFDI"
BASE COUNT         4829 a         3404 c         3716 g         4175 t
ORIGIN      
        1 tttgacggca gagggcctga tctcaaaagc cttttgaata atgcgtttgg gatggacgaa
       61 ggatcggatt atcttgaaag ccttgctgac aataaagtaa taaagtttgt agcgggtacg
      121 gattcttata tggtcagaat gactgtaaaa cagttaaaac agacaagata ttattatccc
      181 aatgggccgg atggggcaga gatccgtgat actgcaaagg gttcaagggg aagcgaagca
      241 tcaagacaag gggcaattcc ggtaccggta agaatacatc tgggtgaaac gggagctgaa
      301 ggtggagaaa cagtattaag agtcgggcag gtagctccca acgagcagaa ctggcctctt
      361 gcagtaaaag atattgccag gggacaaacg gcggacaaat aataattgaa gccagccccg
      421 cacaaaagat cccgagccat ataacaacat ctgtaagcgg cagaacagta gtttatcccg
      481 gagaaaatat aaaactgtac tctccacaaa taatatccac aaagatatat tatacgacaa
      541 atggcatact tcccactatg gattcggata tttataatta taatacttat gggacattat
      601 ccagcagaac aataacagtt ccggctactg tcggtggtac atttactgtc aaggcatttt
      661 caatgaaata cggcaaactt gacagcgatg ttcagacttt ttcatacaaa ataatttccc
      721 ttccaaaaca aggagggaca tatacggtgg gaagcagtaa atataaaata acaggatctg
      781 cagcatcagg cggaagagca gcatatatgc gcaggatcaa aaaaacatat aaaatttcat
      841 ctgtaccggc aagtgccaag ataaacggat ataccttcaa aataacatct gtggcagaca
      901 aggcattcag aaaaaataag aagctgaaga aagtgacaat aggaagtaat gtaactaaaa
      961 ttggcaaaca ggccttctac ggagataaaa aattaaaaac aatagtcatc aagagcaaga
     1021 aaataaccaa atgcgggaag gcagcattca agggaataaa taaaaaggca aaaataaaag
     1081 tgcccaagag caagctgaaa aagtacagaa agcttttcaa aaacaaaggg caggcaaaaa
     1141 cagtcaggat cgtcaagctg taaagacagt ttggcgattt tgtttatgta gtatataaag
     1201 gtaaataaag gataaagata ttgaaagcag tgtattcgag tgatataatt tatttaatat
     1261 tcggacacag gaaggagtaa aaaaatggga aacgatatgt ttggcggact aatgaaagga
     1321 ttatccagcg ttcttccgca ggatgatccc aacataaaaa aatttactgc acaaagcaag
     1381 atcaacgacc tgaaagaaga agagaccggt atttatgcgg aattggggaa aaaaataata
     1441 gaagaggaac ccggcaaata tccgggattt gaagaaagac taagagcgat ccggatagaa
     1501 cttaatgagg cggagagcgg agcgaaaaag gcagatatgg aagctaaaga agctgagaaa
     1561 gtcaaggaag cagaaaatgc aaaaaggaca tgcccaaact gcgggactat caacgaagaa
     1621 ggggtaaaat tctgttctga atgcggcaca gagataggtc agggactcac gtgccccgga
     1681 tgcggagcgg ctctttctcc cggaactaaa ttttgtggtt catgcggagc taaggtagga
     1741 gaatagagat ggctacatat aagcagccct gcatacactg cggaacattt attgaacgca
     1801 gtagcagttt ttgtccgaaa tgcggcagca acagtccatt cggctacagc tgtccctcct
     1861 gtggtcaaag catagaaaaa gagcaaaagc ggtgctcaag ctgcggcagg gatctttatg
     1921 tgtcctgccc gcattgcggt gaactgactt ttgtacagga aaaatgtgag gtctgcggca
     1981 aaagccttat gaaaatatgc ggaaacagaa ggtgcaaggc agaacagttc tttgatttgg
     2041 tcaaatgtac tgcctgcggc aaaagcctta taccggagac cgaaaaaaag ggatttttca
     2101 atagaaaaaa gcaggatgat tgatttggaa ggagactaac aaaatgatga aagcatttac
     2161 aaggaattat gatgataatt ccaccgaagc cggctttcag tttacatttt attgtgatat
     2221 atgcggcgat ggatacaaaa gcagttttat agaatcagaa acatatgaca aaaaacaaaa
     2281 gatacaggga gtagccagag gagcaggtat acttggaagc cttatgggcg gggtaggata
     2341 taacaccgga tgggcagcag aaagagccgg agacgtgatg gctgaaagat ttgaagaaag
     2401 aagcccggaa tggcgaaaag aatacgaaaa tgcattcaac agagcgcaga atgaggccca
     2461 gcagcatttt cacaggtgtc atgcctgcca gaaatgggta tgtgatgccg attggaatga
     2521 agatgaagga atgtgcgtag aatgtgcgcc aagaacagag atatataccg caaaagccaa
     2581 agctgatgcc atcaaaagga atatagatga aaaggcggct gaagctacca tatgggacgg
     2641 tgatctgaaa agcaccacta ctgtctgccc ttcctgcggg aaacctgccg gtaacggcaa
     2701 attctgcaac aactgcggtg cgtcacttga actgaacaaa tgcccgtctt gcggggccga
     2761 aaacgctcag ggggtaagat tctgtaatca gtgcggagcc aaaatggacg gaccaaaagt
     2821 cacaaaatgc ccgggatgcg gcaaagagat ggaaccggga gccaagttct gtggagaatg
     2881 cggaacaaaa attgaataag aataatagag aacagataga aacagataaa ctatcaccat
     2941 ttattgaaaa ccttttcggc aaagccggga aggtttttta ttttaagtaa agtgccatcg
     3001 tcgagaactg ccttgcctga aaaactgccg aacacctgat gctgatttga acatatgatg
     3061 cccagatccg tgcgggaact tctgtctatg acaggaataa agtccatctc gaaacggccg
     3121 tcatcggagg tgaagctcca gggagaaaga aaatcttctc cgcccatttt atgatccgac
     3181 tgtttcatac cgggtttgtc ggggatattg aatttgaccc gggatatttt gtgaccttta
     3241 tttttataga tgatcatgtt ttcagtggcg gcacttgtgt cgccaaagcc gtatccgata
     3301 ttgaagccga aagggctgcc ctcatgaaga cctgatgcgc ttccccagaa ccaggtattt
     3361 ttataggtcc agacgcccct tccccagtcc aggacaccaa aagaatccga aggatcaaag
     3421 atatgatccc gtccgtcaaa tttcactatg cccgaagcgg gcatacagtt tattttttcg
     3481 ttatagtaaa atgcaagatc gtcttcgttg aaaggggttg ctatgatcat cgaatccata
     3541 tcgggacatt ttagggtcag atctccctct atgggctttc ctttataaaa gtcatccata
     3601 taaaaactta aatgcctgat agaactcctg tgttcaaaac atataaaata atttttgtct
     3661 ttagttttga cattgccttc aagggtggag gagggcattt tctttttacc gaaagtaaaa
     3721 ggctgcattg ggctttttgt gtgctgccag ggtcttaaaa aatccagaag ggagatgctg
     3781 tcaaggccca tatatgagtt gtctgccaca gttagagcta tgccgtattt ttcatttcct
     3841 atatagtaat aatcccattc cttgatcctt cttccggaag ccttgatgtg gctgcggtta
     3901 tatacctgca gatgacgtct cgaatagccg ggagcggaaa gatgtccttc cggtgtcagc
     3961 agattttgtt tttttgttat ttctttctga ccggtatttt catttataaa actgtttttc
     4021 ataagacctc atcctgcatg ccttcatata ctgttttgag tatatgaacg atcaaaagta
     4081 ataactacaa aataacaggg atcgattttc tgaagttcat cctgtacttt tcctttgatt
     4141 tctttctctt ctgtcttgca ctcatgatca agcaccaggt caaaaattat gtttgtatgg
     4201 gtaggaccgg gaactacgcg aagatcatgc atacccgtga ctccgggaaa tgattttatt
     4261 gtttctgtta ttacaggttc aacttttttt acagcaggat cgtttctttt gattggatcc
     4321 atatgggcca cgcaatgtat tccgagggtt tcaccgatct cccgctctat attatccacc
     4381 atatcatggc ttttcattat atcctcatcg gcatcgacct ctatatgtat agaagcaaag
     4441 actttgccgg gaccatagtt atggaccaca aggtcgtgga tgccgataac tccaccggga
     4501 gcagaagcca ttttttctat ctcacttacc atgtcctcat cgggggcttc tcccagcaac
     4561 ggacttgaag tctcctttat tagagttata ccggaccaga ttataaaaag tgcaacaaga
     4621 gaacccagcc atccgtccag ttgaagacct gtgaatttcg ttattattac gccgataagt
     4681 acagcacttg tggcgataac gtcgtttctg ctgtctgttc ccgtggctat caaagtcatg
     4741 gaattgattt ttttgcccac atacatattg aaaaaagtct gccagacttt gatcaatatg
     4801 gccagaatca ggactgttat agttatatag tcaaaagaaa gaatttttgg atcaatgatc
     4861 ttagatacag aagatctcaa aagaaaaaga cccactatga taatgagtat ggaaataaaa
     4921 agacccgtga gatactctat tcgggcatga ccgtagggat gatccttgtc ttccggctgg
     4981 gaagccattt tgaaacccac gagagttatt atggaagatg aggcatccgt caggttattt
     5041 actccgtctg cgatgatggc tatgctggaa gaaatgagac ctatcaatat tttcatcacg
     5101 caaagaagaa ggttagaaat tatacccact ataccggcaa atttgccgta attttgcctg
     5161 actttgggat cctttacgtc ttcgtagttt tttatgaatt ttcttataag aaatttaccc
     5221 atttatatta tttttcccgg ttttcggaaa gtgctttatt taaggcaatg gtaagctgat
     5281 ccgcacagga tgttgatttc atgccgcagg tattgccgtt gagtttttcg gttatctctt
     5341 ttacggtcat gccgtcaagc aaagagctca ttgccttaag gttcccgtta catccgccga
     5401 agaaattgat atttttgacc acatcgcctt ccaggtcaaa ttccagtttt gtagggcaga
     5461 cgcctcttgg gatataagtg tgtttcatgt ttttttcctt tcatatgcat caaaaatcag
     5521 tataatatat tatatcacaa gaagcaggga ggcaaacatg aaagttgtat gtgcaggtaa
     5581 tagtattgtt aacggattcc ctcaccgcag aagttgcagt tttccttctg tactcaggga
     5641 aatgaccggc tggaaagtta taaacaaggg aatcaacggc gaaaccacag ttatgctggc
     5701 agaaaggttt gacagagacg tgcttctcca tgaacccgaa aaggtcatta tacttacggg
     5761 cacaaatgat tttattttcg aaactgaaac tcccgaaggg gccatgaaaa acatagcccg
     5821 gatggcagag aaagccaaag aaaaaggagt ggaagtcgta cttctgacac ccataaactg
     5881 taacgaaaca caggcggcgg aatgctggat gaaggggtcg ggcacagatt atcatgaagt
     5941 caacagaaag ctttccaaac tggcggaaat gataagggat catggtagca tgaattgttg
     6001 caaggtggtg gatttagagg tagaatataa gaaattcgca aaatttcatg acgggataca
     6061 tccgacagta gaaggacagg cttatatagc acaaaaagta aaggaaggta tttgagatgc
     6121 gttcagacgt aacaaaaaag aaaaaaagca aggcacctat cattatactt gttataatat
     6181 taatcgccgc acttggcgca ggggcatatt ttttattatc aaaggataat aaaaaaagcg
     6241 gaggcataat atccgatgag ataataaatc cgcttaccgg cgaatctgtt gaggaactgc
     6301 tgccgaggcc tatacagttt tcaatcgata atgccgctcc cgcaagacct cagtcgggtc
     6361 tttctgcggc agatatcgtg tttgagtatc cggttgaagg cagcataaca aggatacagg
     6421 caatatatta tactaagctt cccgaaacgg tgggacctgt gcgcagcgca aggccttatt
     6481 ttgtggatac ggccagagag tataaggcgg tcttcgtgaa tcacggatgg tccccggagg
     6541 caaaagaata ccttgaaagc ggggtggttc cctatatctc cgggctcaca tacagcggtg
     6601 gagtttttta cagggcggat gacagacccg caccggacaa ttcaatgata catacagata
     6661 aacttcttga agccatcgaa aaagaaggat atgacgaaga gcaggatgta aggactttca
     6721 actttcttga aaaaggacaa aaagtcaaag gacaaaaagc gacagagatc aatataaact
     6781 acctgacaac aaagtgctca tacgaatatg acagagagaa aaaactgtat aagaagtttt
     6841 ccaacggcga agtatatata gacaaagaga caggcgatca gataacagcc gcgaatgtta
     6901 tgatccagtt tgtaaaaagc caggtgcttg acaacaaagg aagattgggc atagacatga
     6961 aagccggagg agatgcgatg ctgtttcgaa agggtaaagt cataaagggt acatggtcaa
     7021 gggaagacat ggattccccc acgattttta cagcagccaa cggaaaagaa atgaaaatgg
     7081 caacaggcaa tacttggata caggtgctgg atcagaatca caaaatagag tataactcag
     7141 acgaagaaga acaataaaaa acaagcggcg gagactttaa gtctgccgcc gcttttgttt
     7201 tgtatggcaa agttattagt ttaccagttc ctgaaggact tggttggcaa cactgccggc
     7261 aacattggca ccgcttcccc cgttttccac cagcactatg aaggcataag gattcttttc
     7321 atctctgaga aaacccgtga accacgcgtt gggagaattg cctcccccca cttcggcagt
     7381 accggatttc gcacatatat caagatccgg gaaattatat tcgccgtatg tttcaatgac
     7441 attattgtgc atcatatccg aaagcaccga ggcggtttca gattcgataa gagtatctgt
     7501 tttacggctc cagtttattc cggcgggaag accgctgttg gttttgatgc tgtctataag
     7561 attgggcact gctgcttttc cgccatttgc tattgctccc gcatatatca tcattgaaca
     7621 tggatttaca agatcgtgat actgtcctat tcccgtccag gcaatattca cgtcctcgga
     7681 aggaaagtca aatgaccccg gtgtcgtatg gatgccgttg atattgtagc tgtccgttaa
     7741 gccggctttt tttgtgtatt tctttatgtt gtctattccc agagaaaggg tcagatccgc
     7801 aaatccacag ttgcaggaaa cagcaagagc ctcttcaaaa tcaacttcgc cgtgggggta
     7861 aggacaggtt atcctgtcca cctcggaaga ttcgaactgt tttttgccgg tgcagttata
     7921 tgtccagctt tcgagatcct tctttttttc tattgcggcg gcggaagtca ccagtttaaa
     7981 aatggaaccg ggaactatcc tggcggtagt gcatctgtta agatataccc cggaagtatc
     8041 atcctctttg atctcgggcg gatccgtcgg atcgtaatta ggcgaactag tcatacacaa
     8101 aacatctcct gtcttgtagt tatagaccat cacggctccc ttgtgatctc ccagagcttc
     8161 gcttgctgtt acacaggcat ccgcatcgat agtcagatat atctgtctgc cttcgccacc
     8221 tgctgaatac gtgccggtaa caggattgta tcccacgagt ttgccggcaa aggcccggtt
     8281 ggctcctgtt agtatatttc cgtcgatatc ccccactgca tgaagtgtgg cagtacgcac
     8341 actgtaactg tcgctgtatt cgggctcgcc gctttctgtg ttttcaagaa gcacctgacc
     8401 gtttctgtca tatatggacc ccgtattgat tttcccgtcg gtgaaaacat gtttgttggc
     8461 aggatagctc acccattcgg aaccgtaaac cagccacttt cccacataga aacaaagacc
     8521 ggcagccagc attcctgcca aaaggagaca catcagtgct cgtctttcaa gctttttcat
     8581 agcgtacctc cttctatttc ctcttcatct tctattttgc gcacagctat actggcaccc
     8641 tgcctggtgt ctgcggcttt aagaaatgcc agcagtcccc atgaaacaag catgctgctt
     8701 cctccgtttg aaacaaaggg gaatgtaaca cctgtaagag gaaagatatc aacggcaccg
     8761 aatacgttaa gcatggtctg gaatatgaac agggaagttg ctccgcatgc ggctatcgta
     8821 taaaaagttg aacgtccccc catgatggag cggacggcga atacaccaag agttattatg
     8881 gcaagcacag ccagaatggc tataataagt ccccattctt cgcaaagtat gccgaaaaca
     8941 aggtctgtgt cggaagcaaa aacattatgc agccagccgt caccggaacc gtatcccaca
     9001 aggccgccgc tggcacctgc cgacatggtc cttgtctgtt gaaaaccgcc cgtatcggca
     9061 tattgccagg catgtcccca ggtggcgaat ctttcagcta tataaggacg gaaccgcagg
     9121 accataagac ccgcagcaaa agctcctccc gctgtaagta tcagttttga aaactcaccg
     9181 cttctcagga aagagataac aaggtatgta acaaagaata tggcagccgt accgaaatcc
     9241 cccattactg ccagacaacc cagacagaaa agagagaaaa gcatgaacag cccaagattt
     9301 ttcttttcgt aaagttcgtc caatgtggca gacccgataa aaataaaggc tatcttgaca
     9361 aattcagagg gctggaaaga aaatcctccg atggacaccc agtttgcggc accgtaggtc
     9421 agtgttccga aagccagatt tatcagaagg agcaacacgc tggccgccac aaggatccgt
     9481 cttataagaa gagtcctcga aaggtctctc agatatatgc aaaggcagaa gaaaagcact
     9541 actcccagta ttatggttat cagctgtttg ctgacttcgt tgggagcagc tgttgcggtt
     9601 atggcaagat tgagtgtaca caggaaaaac gctattactt ccatttcaaa atttgtgctt
     9661 cgcatcactt tcagaaaggt gcagaatgcc cacatcagaa cagtcagtcc ggcaaaactc
     9721 agaggtacgg taatgggaag ttctccgcct tccgcaacaa tgagctgaag acaaagcagt
     9781 atctggaaaa tggtaagaga cagcagaagg gtccagggag ggaatttgcg actgcgtttt
     9841 ttccgccggc ccagatcttc catcttttcc tgaagacttg caggtaccag tacacagttc
     9901 acgcctgcca gagatatggt atcaccggcc tttacgggag tggatctgta tacatgtttt
     9961 tcgtttatca tggatccgcc cttggaaccc aggtcattat aacgccagtt accgtcctca
    10021 tcccggcaaa gtgtaccgtg attcctggat acagtaagta tatcaactac aacatcagca
    10081 ctttttgcac ggccaataac attttcccag tgagtaagag ggacatggct tccgtcgggc
    10141 aaagcaagaa aggcccatgt ttcaggtgtg ttttttgcct ccagcagaga gcgtatggac
    10201 ttgaacagaa taaaaacagc cagtatgacg aaaatccacc tggcagctgt agtgaagtat
    10261 acagcaaaag tcggatctga ctgaaaaaca tcggatatct tttccataaa aggttttgat
    10321 gcttccgaaa gtgaagtgaa taattccaac gctattccct cctgcgcatt attatatcat
    10381 aatcattaat aattagttga aaaaagggtg aaaaataaat ataataatgg ggaatatcca
    10441 tatagggaga atcaacatga caaaaacaaa aaagagatgg ggagacagaa aagacgggac
    10501 acgtttaagg gaaatagacg gacttcatca gaccatggca catctccttc caaaccgctg
    10561 tgatgctgaa attttcataa aagaaacaat ggatgtaacg aatattatcc gctatatcga
    10621 agaaaaaaag gcggaggatc cggcatccag gttgaaaacc ttccacattt ttgtggcagc
    10681 tatagcaaag acggtatatc acagggaact gctgaacaga ttcgtggcgg gaagaagatt
    10741 ttatcagaga aatgtcgttt ctttggcatt tgtggtcaga agaagtttca atgatgaatc
    10801 ggaagaatcc cttcttgttc taaaagccaa cggggaaaca gaactaaatg atatcagccg
    10861 caagataacg ggagatgtaa aggaaatcaa agaaggagaa aagggagaca ttgataaagt
    10921 gctggacttt tttgcccgac tcccgaggcc catgcagatg ctggtggtca aacttgccag
    10981 gatacttgat tttttcggca aaatgccgga agcaataaca agcggagaca caaattatgc
    11041 ttctgtactg ctttccaata tcggcagcat aaaatgcgga gccccctatc accatctcaa
    11101 caactacggg acaaattcca tcatgataac gatcggagag atacataaag aacaggtgcc
    11161 tgacaaagac ggtgttcctg tcatacgcga tgtggtggat ttcggtatca cactggatga
    11221 aaggatagca gacggatttt attttgcaag atcggtaagg ctgctaaagc atattataga
    11281 aaaccccgtt ctgctggaag aacctttgag agaggtagta gaatatgaag gttaaaacac
    11341 catggtactc atcatacgga aatgtaccaa gccatctgga ttatcccggc tgctccatga
    11401 tagagtatat agaaaaaaac gccaaagaat acccgcagct cacggcctat gaatttctgg
    11461 gaacttctgt gacttatgaa gagtttctga tgcaagttca tcagtgcgcc aaagccttga
    11521 gagcgacagg tatagaaaag ggagacagag taacgatctg catgcccaac actccccagg
    11581 gagtgataat gttttatgcc gtcaatctta tcggagccgt ttcaaatatg gtgcacccac
    11641 tttccgcaga aggagagata gagtttttta tcaacatttc caaaagcaag gctattcttg
    11701 tactggatgc attctatcag aaagtcagaa acataagaga aaaaacaccc tcccttgagc
    11761 atgttatcgt tgcttcaata gccactgaac ttcccccaat aaccaaaata ggattcaagc
    11821 tcacaaaggg aagaaagatc ccaaggatcc cggcaggaga tgatttcgtt tcatggactg
    11881 actttatgtc ttccggtgaa agatatattg aagaatatat tgcccacagc agggcagatg
    11941 atccggcatc catactatac agcggaggca cctccgggaa aacaaaggga atagttcttt
    12001 caaatctgaa tttcaatgca ctggccatgc agaccggcgc agccagtgag accatagcac
    12061 cgggccagaa gatgcttgca ataatgccca tgtttcatgg ctttggcctt ggcataggca
    12121 tacatactat gctgcagggt ggatgctgct gccatctggt tccccagttc agcgtggaaa
    12181 cctatgcaaa actgctaaga aaggtacagc cgaattacat agccggagtt cccaccatat
    12241 atgaagccct tttgcgtaac gagaatatga aaggcgttga tctggcatgc cttttgggag
    12301 ttttttccgg cggagattct ctttccatcg aattaaaacg caaggttgac aatttcctca
    12361 aagaacatgg agccaccatt cagataagag aaggatacgg acttaccgag tgtgtcactg
    12421 ccagctgcct gacgccaaaa gacatatata aagaaggcag catcgggata cccttcccgg
    12481 atacctatta taagatagta aaggccagca ccaagaatga agtgccgttt ggagaagaag
    12541 gggaaatttg cgtaagcgga ccatcggtaa tgatggaata cctggaagat gaatctgaaa
    12601 ccgaatctac gctgcagaga catgaagacg gactgctttg gctccacacc ggagatcttg
    12661 ggatgatgga tgaagaggga ttcgtttatt tcaagcagag actcaagagg ctgataatct
    12721 catcgggata ttccatatac ccgtctcagc tggaaaacat aatagatgcc aacgaaaatg
    12781 tgcttatgtc cacggtcata ggagtgcctg atccatacaa aatgcagaag gtaaaggcct
    12841 ttgtggtcct caagaagggc atcaaggaaa cggaagaaat caaagaagaa atttatcagc
    12901 actgcctcaa gaacatagcc agatatgcca tgccttatga attcgaatat cgtgaaaaac
    12961 ttccccagac tctggtggga aaagtggcct acacagtcct tgaaatggag gagcatgcca
    13021 agctccgcca gatgcaggac aaaagtaaaa agaaggacgc caaagacaac aaaggcagta
    13081 ataaaacgga agattaaatt ttcagcccga aaaaccggtc aattcaggaa gtgcagcaaa
    13141 tctgtcaagg ccgctggtgt ggcaaacaga gcaattttgc cggtgtaacc ggaaggccgg
    13201 caaaaaatca aatatcaaat aatttctgta cataatgaac agtagccatg gcgtcccttg
    13261 catagaagtg ggcgccaatt tcttttgcat agctttcgct gaggacagcc ccgcccacga
    13321 tgaaacggca ttcatcgtaa aggcctctgt ctctgaacaa gtctattgtg tctttcatag
    13381 agggggctgt ggtggtcata agagcactta agcctataat atttattttt tctttttccg
    13441 cagcttctgc gattttctca ggcggcacat ctctgcccag gtccacgact tcatatccgt
    13501 agttttccag aagcaccttc acgatatttt ttcctatgtc atgtatgtct ccgcgtacag
    13561 tggcaagaat cacccgatcc ttctttttct gaacatcgga tttcatggca gacttcaaaa
    13621 ccgcaaaaga actttgagcg gcatcggaac tcatcagaag ctgcggcagg aacagactgc
    13681 cgtcttcgaa gcctctgccc actttattca gagcagggac aatctggttt tcgattatat
    13741 caagaggaag tttgtttttc aaaagctccc gggtaagggc agaggctttt tccttcaaac
    13801 cttccactat ggcctctttt agagtggaag catctccggt tttgtttttc atgttttcaa
    13861 gactgcactt ataggagtcc cgggcatctc tggatccatc cgcagcggcc atcatttttt
    13921 ctgaaaaagg atcgattatt cccatatcaa gaccacggct gacagcttct ctgtagaaag
    13981 cagaatttac ggaatccctg tcaggtaggc cgaaagaaat atttgagaca cccaaaacag
    14041 ttgctgtgcc gagttccttt tttatcagct ccatggcggc cagggcagtc tctcctgcat
    14101 tttcatctac gcttatagtc atggcaaggg ggtctattat gacatctttt ttatcaatgc
    14161 cgtattctgc ggcggtttct ataatttttt ttgctatgac aagacggcct tcgggagttt
    14221 tcggtattcc ggtttcatcc aatgttaggc ctatcacaac gccgccgtat ttttttacaa
    14281 gtggaaaaac cttttccatt gaatcttttt tgccgttgac ggagtttatt acggctttgc
    14341 cgttatatat cctaagggcc tcatccatag cttcaatatc cgaagtgtcg attataagag
    14401 gaagagtcac aatactttgc acttccttga tcaaaagagg aagcatggtt ttttcatcta
    14461 tttcaggaag acccacattt atatcgagta tgtccgctcc tgcttgctgc tgctttgccg
    14521 ccagctcttt tacggtggcg gtatctcctg cctttaaggc ttccttgaca agtttgtttc
    14581 cggtgggatt gattttctcc ccggcttttt tatgccggtt tacggtttta tccccggggc
    14641 gggagcctat acggacagcc tgtgcatagg aagccgcaaa ggtctcatgt ttttttgtaa
    14701 cggcaggagg aacaacattt ctgcaaagtt caatttcccg tctgatatgt tccggggtcg
    14761 ttccgcagca gccgcccaaa agccaggcgc cggattcagc tatggaaaca gcttcctttg
    14821 aatattcttc cggtcccatt gtgaaaacgg tttcaccatt tattgatcgg ggaagccctg
    14881 cattggggtt ggccatcaca ggaacagaag catatttgaa aaattctttg acgatgtttt
    14941 ttatttgtgc cggcccaaat ccacagttgg tgccgatgac atcagcaccc agaccctcca
    15001 ttacagcaac ggcagttttc acatcgccgc cggtcaggta ttttttgttt tgttcaaagg
    15061 tcatggaagc gatgacggga aggctgcagt tttctttggc ggcgaggata gccgctttca
    15121 tatcataaat atcaggcatg gtttctatta tgatggcatc ggcaccttcg tcggcgccgg
    15181 cgtgaacaag gtcggcaaaa actttatagg catcatcgaa actcaattcg cccaaaggct
    15241 ccagcaagcc gccggatggg cccagctctg cagccacaaa aactttttcc ttgccttgga
    15301 tcctgtccgg atctccggct gttttggcat tgtacttttt tactgcttct cttgccagtt
    15361 ttacgccggc ggccatatat tttgcatccc tggctctgaa cgtatttgat gtcaatatgt
    15421 tgcatcctgc gtccagaaat ttttcgtgta tatcaagaag cacctgaggt tcttccaggt
    15481 tccagagttc aggagatctg ccgcctttaa ggcctgccgc ctgaagcatt gtacccagcc
    15541 cgccatcaaa aaaaagcagt tcttttccaa aaagttcttt gatacccata cgatcctccc
    15601 tgtttgacat gacctttcgc ttatgttttt tctgacaatt tttcctttga tatttgttct
    15661 gatatttctt cttttatatt tttccagtat ttttttggat atttctccgg agatcagtaa
    15721 ttgaaacaat tatagcataa aagctgatta tttgcgttat gtgtttacag tcacttctct
    15781 taaagggcga gaaaataaaa gagggcggaa tatggtcgat tattttcagt tgtgctcagc
    15841 tgtgttcagt tgtggtggat tcttttcact tgttttcagt tgtggtcggt tctgatgtgg
    15901 tcggttctga ccgatcccga ccgattatgt tttcaaaata gagatgtttg gcaaaaaaca
    15961 gtcaaaaaaa cataagtgat ttgaaaattt aacccttttt gcctcagatc agctcctcca
    16021 gggttaagaa taaggtaaaa gacggctttt ggccgtcttt ttaccgtcta aaaaggtcaa
    16081 gtccattttt gtgtgtaaat tcgttcggtc gtcatctgca ggcg
//
LOCUS       DAONHK010000007        15954 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_2875_length_15954_cov_5.218253, whole genome shotgun sequence.
ACCESSION   DAONHK010000007 DAONHK010000000
VERSION     DAONHK010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 15954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15954
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2875_length_15954_cov_5.218253"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            68..1471
                     /locus_tag="PLM92_00630"
     CDS             68..1471
                     /locus_tag="PLM92_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005999188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HPR23683.1"
                     /translation="MNIREYEELLKQMTGIIKEAVHVVDAGGRSIIYNQAMANLEETR
                     REEVLGTLFADTFSNIPKEESTMLKALRHGKATMNKQQTYLNKYGKEITTVNSTVPVT
                     VDGKIVAAVEIARDITDIKKMSNTILELQEESIKPKKTEYPGIKHYNFADIIGEDPEF
                     VKLVDSAKKASHNKASVFIYGETGTGKELFAQSIHYEGSRKTGPFLAQNCAALPASLL
                     EGILFGTSKGGFTGAVDRAGLFEQASGGTLLLDEISAMPYDLQSKLLRVLQEDYIRRL
                     GGMKDVPVDVRIIATVNEKPEILIEKGALRKDLYYRLGIVNLDLPPLRERKGDIPVLA
                     HELLEKQSRRFKKKVSGFSEEAMTKLQSHDYPGNIRELENIIMAAVSMADEESLLDAE
                     HIILPAALYPKSGKKDTVSYNSGIEKMKGISLSGHLDIIEKELISEAMEKTEGNISRA
                     ADMLGISRQLLQHKLKK"
     gene            1736..2794
                     /gene="ord"
                     /locus_tag="PLM92_00635"
     CDS             1736..2794
                     /gene="ord"
                     /locus_tag="PLM92_00635"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005999189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,4-diaminopentanoate dehydrogenase"
                     /protein_id="HPR23684.1"
                     /translation="MQNVKVIIWGLGAMGGGVADMLLSKKGVDIVGVAGRGKKIGTSM
                     YDYIKTEKGDREDVIIGDAEDVIKPGSADIVILCTDSFTKNAFPRLKFIMEQGINVIT
                     SAEEMSYPKAKEPELTEELDKIAKKNGVSCLGTGINPGHIMDMLVLIMTGCMVDVEHI
                     TSRRVNSLSPFGPAVMEEQGIGIAVEEFKKRKAEGTMAGHVGFAESVGMMAEGLGLEV
                     EKFSQDMNPITTDVDRKSPYGFAKAGSCAGVSMEADAKLTGGMEIRMEHPQQIEPEQV
                     GVQTGDYVIIKGTPAINMVNSPEVEGGLGTIAMCANMIPQVINAKPGLHTMLTLPIAH
                     GIMGDMRKFIDEDCKIVK"
     gene            2805..3104
                     /gene="ortA"
                     /locus_tag="PLM92_00640"
     CDS             2805..3104
                     /gene="ortA"
                     /locus_tag="PLM92_00640"
                     /EC_number="2.3.1.263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007488541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-oxopentanoate thiolase subunit OrtA"
                     /protein_id="HPR23685.1"
                     /translation="MIKKGEWVRIHKNILEPSERAPQVPDDTKECPLEMWDKGFLTAD
                     ADMGDEVEIETVANRIEKGTLIEVNPSYDHDFGKCVPELLKIDKQVREILFGGEK"
     gene            3104..4510
                     /gene="ortB"
                     /locus_tag="PLM92_00645"
     CDS             3104..4510
                     /gene="ortB"
                     /locus_tag="PLM92_00645"
                     /EC_number="2.3.1.263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010255805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-oxopentanoate thiolase subunit OrtB"
                     /protein_id="HPR23686.1"
                     /translation="MVKANDYTSVMGRKAEIMLRSVGIDYSKFESGSIAFDYEKMMKE
                     TGYTLEEMQEIQYGVNVGNTPLLELKNLTALARSCAPRGKGARIFIKDEASNPSGSFK
                     ARRAANAVYHAKKLGYKGVIAATSGNYGAAVASQAAIAGLKCIIVQECYDSKGKGQPE
                     IVEKARKCEALGAEVVQLSVGPELFYKFLLLLEETGYFNASLYTPFGIAGVETLGYEV
                     SMQFREKYGKDPDVVICTHAGGGNLTGTARGLKKAGAASTVIGASVDLAGLHMASDKA
                     FNLKSFTTGHTGFGVPFSNWPDRSDVPRSAARPLRYMERYVTITQGDVFYMTECLATL
                     EGLEKGPAGNTSLAAAFALSQEYDRDKLIVVQETEYTGAGKHVQPQLSFAKSNGIEVF
                     FGDPKDEIPGKNIVLPEHPSLIKATDVDLDHMRRSLIKTALKTYNTSEPTDQDIKFLA
                     EETMKDEAWVRKTVSELK"
     gene            4554..4916
                     /locus_tag="PLM92_00650"
     CDS             4554..4916
                     /locus_tag="PLM92_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013361504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine aminomutase subunit alpha"
                     /protein_id="HPR23687.1"
                     /translation="MKRKDDFAERRKHIANLSDQELYDRFWDLTTQVVTPMLELGKKN
                     TTPSVERSVLLRMGISSLETKPIVEGCMERGLMGKGAGHVVYKLSKAKGISVKEAGTM
                     LAEGKGWDDAVSLFKKGV"
     gene            4920..7136
                     /gene="oraE"
                     /locus_tag="PLM92_00655"
     CDS             4920..7136
                     /gene="oraE"
                     /locus_tag="PLM92_00655"
                     /EC_number="5.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007488538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ornithine 4,5-aminomutase subunit OraE"
                     /protein_id="HPR23688.1"
                     /translation="MAEIKLKHNEKLDIDNILKDLDKYEPRRSGWTWRKPAPGLKMGP
                     FEYKDASEPLKNGVPLPPAKYFENIDPQPMPVITTEIASGRFEDDIRRMRMGAWHGAD
                     HLMVIRTAGQSHMDNLIEGTPQGIGGVPISRKQVRAQRKALDAIEDEVGRPINYHSYV
                     SGVAGPDIAVMFAEEGVNGAHQDPQYNVLYRNINCVRSFVDACESKKVLAWAEIAQID
                     GAHNANATARDAWKVMPELIVQHAINSRFSELAGIKPENICLSTVPPTATPGPCVFMD
                     LPYAVALREICDRYRMRAQMNTKYIDSSAREATVTHVLNMLVSKLTSADIQSTITPDE
                     GRNVPWHIYNVEACDTAKQTLIGLDGLMEMVELKSDGPLREKAREIKERACLFMEEIL
                     EVGGYFKAVEEGFFVDSAEYPERNGDGIARQIDGGIGYGYIYERDEDYMAPVTAHFGR
                     NNVEQYGGDPKNPSALIGGCTLEDRSKIVYIDELDDVDNVNVRMEETSKYRDTSAMIP
                     EMEWMGDGVVMLTMMIPENRRTAEVAAVEIAAKMGLSDHEVISREVLQDAEGTRIELK
                     GKLGFDIDVSKLVIPPEPHYLSEDEIRADVEAHPLKIVCGTVGEDEHSVGLREIIDIK
                     HGGIERFGIEVHYLGTSVPVEKLVDAAVELKAEAILASTIISHDNIHYKNMKRIHELA
                     IEKGIRDDVIIAAGGTQVIPEEGLKTGVDAAFGRNSHGIDVASFLIEERQRRRKDK"
     gene            7277..8665
                     /locus_tag="PLM92_00660"
     CDS             7277..8665
                     /locus_tag="PLM92_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009258995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlmL-related ornithine degradation protein"
                     /protein_id="HPR23689.1"
                     /translation="MKIDVLVAEIGSTTTVVNAFDKIDTNDPVFWGQGQAPTSVLEGD
                     VRVGLKGAIADLCCRKGIDSLEYDSMLATSSAAGGLKMTVHGLVYDMTARAAKEAALG
                     AGGIIHYITAGRIRRTDLKKIQEIRPNLILIAGGVDFGERDTAIYNAEMIRGMDMDVP
                     VIYAGNVENHEEIKMIFEDAGIKLYIVENVYPKIDDLNVEPCRKVIQDAFEEHIIHAP
                     GMEHVREMVDGPIIPTPGAVMECTKLLNEYIGNLMVLDVGGATTDVHSVTEDSEQIAA
                     LLISPEPRAKRTVEGDLGLYVNRMKVIESIGEDNFRKDLAGIDVEKALESYVAIPRNG
                     DEVKLVERLAEEAVLKAVERHAGTIRYVYGPSGRQTLAEGKDLTQVEYIIGTGGALTR
                     LPRRIELMKKIPKHNESGMMLFPGEGAKILVDNDYIMASLGVLSKRYKEAAVKLLEKS
                     LDFKFPKKKEAE"
     gene            8665..9750
                     /gene="orr"
                     /locus_tag="PLM92_00665"
     CDS             8665..9750
                     /gene="orr"
                     /locus_tag="PLM92_00665"
                     /EC_number="5.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005999197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine racemase Orr"
                     /protein_id="HPR23690.1"
                     /translation="MYPRLFINIEKLKKNIDAVAKITKNDGNCSMMIVTKALCADRKT
                     AEMIGKHPQVDYVADSRISNIRTYSEFVRKNGKETVLMRLPMESEIKDVVRYADISFN
                     SEVSTLEMLDKEAAKQGVVHKVVLMIDLGDLREGMFFENENEIFKAARTVLAAGNLEL
                     FGLGTNLTCYGAIIPKYDNLSVLCTWADRIEGKLNIKIPMISGGNSSSIYLIPKGEMP
                     ERINNLRLGESFLLGNDTAYCTKLDGTTDDALILEAQIIELKEKPSLPIGEVGVDAFG
                     NRPEYENRGTIKRAILAVGQQDTEQSGLHPLDEKVDVLGASSDHLILDVTGSDKKYKT
                     GDVIQFKMEYGAVLKAATSPYVEKVYK"
     gene            9807..10355
                     /locus_tag="PLM92_00670"
     CDS             9807..10355
                     /locus_tag="PLM92_00670"
                     /inference="COORDINATES: protein motif:HMM:NF039801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6506 family protein"
                     /protein_id="HPR23691.1"
                     /translation="MFKFAFIINLPGESPGSFSRIYETKESLSRVLATGSDEMTRETV
                     KKLAEEGYELVNFCGDFDDEMTKRYYEAAEGTIEMRHADYFPKEMEKLEASSSLKEYG
                     IIIIARGTEETYKIKMKSPDCNVNVCFVKDIDSAKTAVCQLVADGIDFIELCSWFDKE
                     KTEVVIEAIGGKVPVGSCGKIN"
     gene            complement(10378..10659)
                     /locus_tag="PLM92_00675"
     CDS             complement(10378..10659)
                     /locus_tag="PLM92_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013380499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HPR23692.1"
                     /translation="MYKEFSGHYVYILECADGTLYTGWSTDIKNRVRTHNSGKGAKYT
                     KSRRPVSLVYCEKYDTKSEALKRERSIKNMSRSLKLELIRSSSVKYNDF"
     gene            complement(10694..11764)
                     /locus_tag="PLM92_00680"
     CDS             complement(10694..11764)
                     /locus_tag="PLM92_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015263133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPR23693.1"
                     /translation="MRIKAVALRILNQIRHDKRTVGIIVVAPLLILTIVYFILDSGDS
                     GYDIGIIKAPDHFVETLEDNEDVDVKVSYYDSALAEKAIKDGEITAAIYIPDNENDLA
                     DYTDIEVLIDGTDASKASRIKALITVAAAEAIKKDIPVPVSFAEPEIETDYIYGSDDW
                     SLFDNYGAPMIGIIIFFLVFLIAGINFLGERTSGTLQKLLSTPIRRGEIITGYALGFS
                     VLAVLQSSIVTFFVIYVLKMPLEGSIWLVLIVNLVTAMCALTLGMLLSTLANSEFQMV
                     QFIPLVILPQIFLCGLFELSGGWEIAGYFMPLYYTTDALTEVMLHGNGLSEIYIDLII
                     LVSLSTVFMAANSLLLKKQRSV"
     gene            complement(11754..12488)
                     /locus_tag="PLM92_00685"
     CDS             complement(11754..12488)
                     /locus_tag="PLM92_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018305324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPR23694.1"
                     /translation="MENCIRLDNINKSFGKKQVLFDVSLKIPKGCIFGLLGPSGCGKT
                     TSVKIMAGILKQDSGSAYIMGGLMPDLSLMAQMGYMAQSAALYPTLSAYENLKFFGSV
                     YGFKGHVLVERIDKVTDLVGLTHELKKTVSAYSEGMKQRLSLAIALLPDPKVLLLDEP
                     TVGIDPVLRKSIWKELYYLSDQGVTILVTTHVMDEAGKCHRLAMMREGVFLAEGTPSE
                     IIEKSGAKTIEDAFIYFGRGGGSDED"
     gene            12619..15237
                     /locus_tag="PLM92_00690"
     CDS             12619..15237
                     /locus_tag="PLM92_00690"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HPR23695.1"
                     /translation="MSRLEKIKTGLPGLDGILQNVRQGESVVWQISEGEDYAYFARHF
                     AAQAIKEGRNVIYFRFSDYPALLEKQEGLKIIRMDLNSGFEKFTVSIYKVISKQDPGT
                     FYVFDSMSQLQTVWAADFMMRNFFKAICPALKEMKGTGYFSIAYKGHSYDSISQIKET
                     ADIYINTVSGPEGIYVQPLKAANRKSPTMFFPHLISDEKAAKLPPITDGISSSKYYGL
                     LKIKARNPGQRFLDNWDVFLMEAQTAMLEESPDMELYEKKLYKMLIGRDQERANLFKA
                     NYNIQDYLNINLRLVGTGSIGGKAAGMLLARKIIENNRPDLAEHIEEHDSFYVGSNVF
                     YTFLIRNNWWKLWLEHKSDEGYFMAARVLKSQIPYGDFPDLVKEKFRRLIDHYGQNPI
                     IVRSSSLLEDGFGNAFAGKYDSIFCVNIGSPEERYEQFVKAVKDVYASAMDESALIYR
                     KQRGLDKADEQMSILVQRVSGSVFQDVFMPGAAGVGYSMNSYTWNKSIDPNEGMIRLV
                     AGLGTRAVDRTFQDYPRLAALDKPALKPSMLPEDRFRFVQKNVDVLDFTENTLLTIPL
                     EEVAEKAPNWYNSLFVEHDYETENRLSDMGRRQQVISTTCEKMLKNEELVSTMREVLK
                     TLHEHYKYPVDIEFTINFSEKGEFLINLLQCRPLQSKGTGIAGVKIPDVPEEKTFMKL
                     FKNTMGGPTKMEFDMVVIVDAKGYAEMPYKEKFSVANAIHAVNTYAGQNKKSLMILGP
                     GRWGTNSAELGIPVRYAQISNVNAIFEMSFESSGLMPELSFGSHFFQDLVETNTFYGA
                     VFEKDSSEGVKSIYRPQVLENEKEVYDEIPDTIKALRNIIKVYELEESKMILVADSKG
                     EETVCWKEKEKTKNSK"
     gene            15433..>15954
                     /locus_tag="PLM92_00695"
     CDS             15433..>15954
                     /locus_tag="PLM92_00695"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HPR23696.1"
                     /translation="MKKNKIKKIIALGLAMALTTGVMSACGGESGSDEQKSSGETVVI
                     AGSTSVQPLSEVMAEEFMADNEGTSVEVQGGGSGQGIKAIEEGIADIGSLSREVKDEE
                     KGSVKEEYIIAMDGVAVVVNTETETEDITIEELEKVYTGEITNWKELGGKDAEITVVS
                     REEGSGTRGAFTEI"
BASE COUNT         5286 a         3030 c         3917 g         3721 t
ORIGIN      
        1 acagacgcaa atatatttgc gcctgttttt gatttgtgtt aaaatagctt tatatttgag
       61 gagaaacatg aatatcagag aatatgaaga gcttctgaaa cagatgaccg gcataataaa
      121 agaggcggtc catgtggtgg atgccggtgg acgaagcata atttacaatc aggcaatggc
      181 aaatctggaa gagacccgca gagaagaagt gcttgggact ctttttgccg acacattttc
      241 aaatatacca aaggaagaga gtacgatgct caaagccctt cgtcacggca aagccaccat
      301 gaacaagcag cagacttatc ttaataaata cggcaaagaa ataacaaccg taaattctac
      361 ggtacctgtt actgttgacg gaaaaattgt ggcagcagta gaaatagcca gagatataac
      421 agacataaaa aaaatgtcta ataccatact ggaactgcag gaagaatcaa taaaaccgaa
      481 aaaaacggaa tacccgggca taaagcacta taactttgcg gatatcatag gagaggatcc
      541 ggagtttgtg aaactggtgg attcagcaaa aaaagcttca cataacaagg cgtcggtttt
      601 catatacggt gaaacaggta caggcaaaga gctgtttgca cagagtatac attatgaagg
      661 gtcaaggaaa accggaccct ttcttgcaca aaactgtgca gctcttccgg ccagtcttct
      721 tgaagggata ctttttggga cttccaaagg gggattcacc ggagccgttg acagggcagg
      781 gctttttgaa caggccagcg gcgggactct gctgcttgat gagataagtg caatgcccta
      841 tgatcttcaa agcaaactgt taagagttct tcaggaagac tatataagaa gacttggcgg
      901 gatgaaggac gttcctgttg atgtgaggat aatagcaact gttaacgaaa agccggagat
      961 actgatagaa aaaggtgcat taagaaaaga cctttattac aggcttggta ttgtcaatct
     1021 ggatctgcct cctttaagag aaagaaaagg agacatacct gttttggcac atgaacttct
     1081 tgaaaagcaa agcagacgct tcaaaaaaaa ggtatcagga ttttctgaag aggctatgac
     1141 aaaacttcaa agtcatgact atccggggaa tataagagaa cttgagaata ttattatggc
     1201 ggccgtatca atggcagacg aagaaagctt acttgatgct gagcatatca tattgccggc
     1261 agcgctatat cccaagtccg gcaaaaaaga tacggtatca tataatagcg ggatcgaaaa
     1321 aatgaaaggc atatctcttt ccggccattt ggacataata gagaaggagc tgataagtga
     1381 agccatggaa aaaacagaag gaaatatttc ccgcgcagca gatatgttgg gcataagcag
     1441 acagcttctc caacataaac tgaaaaaatg atatatcgta aaaaagaaaa atgtccaaag
     1501 atgcaaacat ttttgcatag attatgcaaa tatatttgca tatggacggc agaccctgca
     1561 gtaaaaagta tatttataat caaaaagccc tgattttcag gcttttttta tgtgcaaaag
     1621 cataaaaaca gatggcacag taattgctat atataataga agatgctttt cagataacaa
     1681 aagcatcata aaaggtttac tattttgaaa aacataaatg aaaaggagaa gaattatgca
     1741 gaatgtaaaa gttattattt ggggactcgg agcaatgggc ggcggagtgg ccgatatgct
     1801 tctttcaaaa aaaggtgtgg atatcgttgg agttgccggc agaggcaaga agatcggaac
     1861 atccatgtat gactacataa agactgaaaa gggcgacagg gaagatgtta tcatcggcga
     1921 tgccgaagat gtaataaaac ccggttcggc agacatagtt atactctgta ctgattcctt
     1981 cacaaaaaat gccttcccga gactcaagtt cataatggaa cagggaatca acgtaataac
     2041 ctccgcagag gaaatgtcct atccaaaggc aaaagaaccc gagctgacag aagagctgga
     2101 taaaatagca aagaaaaatg gagtttcatg tctggggaca ggcataaatc cgggtcatat
     2161 aatggacatg ctggtactta taatgacagg atgcatggtg gatgttgaac atataacttc
     2221 aagaagagta aacagccttt cacctttcgg acccgcagta atggaagaac agggcatagg
     2281 aatagctgtt gaagaattca agaaaagaaa agcagaagga acaatggcag gacatgttgg
     2341 atttgccgaa tctgtaggca tgatggcaga gggcctgggg cttgaagttg aaaagttctc
     2401 tcaggacatg aatccgatca caacagatgt tgatagaaaa tcaccctacg gatttgcaaa
     2461 agcaggttca tgcgcaggcg tttcaatgga agctgacgca aagcttacgg gcggcatgga
     2521 aatcaggatg gagcatcctc agcagatcga accggaacaa gtgggagtac agacgggaga
     2581 ttacgttatc atcaagggaa caccggccat caatatggta aacagccctg aagtagaagg
     2641 aggactggga actatagcaa tgtgtgcaaa catgatacct caggtcataa atgcaaagcc
     2701 cggacttcat acaatgctga cacttcccat cgcccacggg ataatgggag acatgaggaa
     2761 gtttattgat gaagactgca aaatcgtaaa ataggagtat aaaaatgata aaaaaaggtg
     2821 agtgggtaag gatccacaaa aatatactgg aaccttcaga aagagctccg caggtgcccg
     2881 atgacacaaa agaatgtcct cttgaaatgt gggataaagg atttttgaca gcggatgccg
     2941 atatggggga tgaagtagaa atagaaaccg tagcaaacag gatcgaaaaa ggcaccttga
     3001 tagaagtgaa cccttcttac gatcacgatt tcggtaaatg cgttcccgag cttctgaaaa
     3061 tagacaaaca ggtaagggaa atattgttcg gaggtgaaaa ataatggtaa aagcaaatga
     3121 ttatacctcc gtaatgggta gaaaagcaga aataatgctg agatccgttg ggatagatta
     3181 ttccaaattc gaatcgggat ctatcgcttt cgattatgag aaaatgatga aagagaccgg
     3241 atataccctg gaagaaatgc aggagatcca gtacggagta aatgtaggga atacaccttt
     3301 acttgagctc aagaacctga cggcactggc aagatcctgt gcaccccggg gcaaaggagc
     3361 caggatattc ataaaagatg aggcatccaa tccttcaggc agctttaagg caaggagggc
     3421 agcaaacgca gtttatcacg caaagaaact gggttataag ggtgtgatcg cagcaacaag
     3481 cggtaattac ggtgctgcag tggcctccca ggcggccata gccggactca aatgcatcat
     3541 tgttcaggag tgttatgatt ccaagggcaa gggacagccg gaaatagttg agaaggcaag
     3601 aaaatgtgaa gcactgggag cagaagtcgt tcagctttca gtgggacccg agctttttta
     3661 taaattcctg ctgctactgg aagagacagg ctatttcaat gcatctttat atacaccatt
     3721 tggtatcgcg ggagtggaaa cgctgggtta tgaggtttcc atgcagttca gagaaaaata
     3781 cggaaaagat ccggatgttg tgatatgtac ccatgcggga ggaggaaatc tgacagggac
     3841 tgcaagaggt ctgaaaaaag caggtgcggc aagcacggtt ataggtgctt ctgtggatct
     3901 ggcaggactt catatggctt ctgacaaagc cttcaacctg aaatccttca caacgggaca
     3961 tacaggattc ggggtgcctt tttccaactg gccggacaga tccgatgtgc ccaggtctgc
     4021 agcaagaccc ttaagatata tggaaagata tgtcacgatc acccagggcg atgtctttta
     4081 tatgacagaa tgcctggcaa ctcttgaagg acttgaaaaa ggccccgcag gaaacacttc
     4141 tctggcagcc gcctttgcac tgtcccagga gtatgacagg gataaactca ttgtagttca
     4201 ggagacggag tatacgggag caggcaagca tgtgcagccc cagctttctt ttgcaaagag
     4261 caacggtata gaggtattct tcggagaccc aaaagatgag atacccggca aaaatattgt
     4321 actgcccgag catccgtctc ttattaaggc cacagatgta gaccttgacc atatgagaag
     4381 atccctgata aaaactgcac tgaaaacata caacacatct gagccgacgg atcaggacat
     4441 taaattcctt gctgaagaga ccatgaagga tgaagcttgg gtaaggaaaa ctgtcagtga
     4501 actgaaataa agataagccc gtcagggcaa taattttgaa aggattgata aggatgaaaa
     4561 gaaaagatga ttttgcagag cgcagaaaac atattgcgaa cttgtcggat caggaacttt
     4621 acgacagatt ctgggatctt actacacaag tagttactcc catgcttgaa ctgggcaaaa
     4681 aaaacacgac accttccgtt gaaagatccg ttcttctgag aatgggtatc tcttctcttg
     4741 agactaagcc tatcgttgaa ggctgtatgg aaagaggtct catgggaaaa ggcgcaggac
     4801 atgtggtata caaactttca aaggccaaag gtatttccgt gaaggaagca ggaacaatgc
     4861 ttgcagaagg aaaaggctgg gatgatgccg tatcactgtt caaaaagggg gtgtaaaaaa
     4921 tggctgaaat aaaactgaaa cataatgaaa aactggatat tgacaacata ttaaaggatc
     4981 ttgacaaata tgaacccaga agatccggct ggacatggag aaaacccgca ccgggactta
     5041 agatgggccc ctttgaatat aaggatgctt cagaacctct aaaaaacgga gtacctctgc
     5101 ccccggccaa atattttgag aacatagatc ctcagccaat gccggtaata accacagaga
     5161 tcgcatccgg aagatttgaa gacgatatca gaaggatgag aatgggagca tggcacggag
     5221 cagatcatct tatggtaata agaactgccg gtcagtccca catggacaat ctaatagaag
     5281 gaactcctca gggcataggc ggagtgccta tttccaggaa gcaggtcaga gcccagagaa
     5341 aagctcttga tgccattgaa gatgaagtgg gacgtccgat aaactatcat tcatatgtat
     5401 ccggagtggc aggacccgat attgcggtaa tgttcgccga agaaggggtg aacggcgccc
     5461 atcaggatcc ccagtacaat gttctttaca gaaacataaa ctgtgtaaga tcttttgttg
     5521 atgcatgtga atccaaaaag gttctggcat gggcggagat agcccagata gacggagctc
     5581 ataatgctaa tgccacagca agggatgcat ggaaggtgat gcccgaactg atagttcagc
     5641 atgccataaa ttccagattt tctgagctgg caggaataaa acctgaaaac atatgtcttt
     5701 caacagtgcc tcccacggca acaccgggac cttgtgtatt catggatctc ccctatgcgg
     5761 tggcactcag agaaatatgt gacaggtaca gaatgagagc acagatgaat accaagtata
     5821 tcgactcttc agcaagagaa gccacggtga cacatgttct taatatgctg gtgtccaagc
     5881 tgacaagtgc tgatatccag tccactataa ctccggacga aggaagaaac gtgccatggc
     5941 atatatataa tgtagaagca tgtgacacag ccaagcagac acttatcgga cttgacggtc
     6001 tcatggaaat ggtggagctg aagagcgatg gacctcttcg tgaaaaagca agagaaataa
     6061 aagaaagagc atgtcttttc atggaagaga tacttgaagt cggcggatat ttcaaggccg
     6121 tcgaggaagg ctttttcgtt gattccgcag aataccccga aagaaacgga gacggcatag
     6181 caaggcagat cgacggtggt ataggatacg gatacatcta cgaaagagac gaagactata
     6241 tggctcccgt tacggctcat ttcggccgga acaatgtaga gcagtacgga ggcgatccaa
     6301 aaaatccttc ggcactgata ggcggatgca cacttgaaga cagaagcaag atcgtttata
     6361 ttgatgagct tgatgatgtg gacaatgtaa atgttagaat ggaagagaca tcaaaataca
     6421 gggatacttc tgcaatgata cccgaaatgg aatggatggg agacggtgtc gtcatgctga
     6481 caatgatgat acccgaaaac agaaggacag cagaagtggc agctgtggag atagccgcca
     6541 agatgggact ttcggatcac gaggtaataa gcagggaagt tcttcaggac gctgaaggga
     6601 caaggataga acttaaaggc aaactgggat ttgacataga tgtatccaaa cttgtcatac
     6661 ctccggaacc ccactatctg tcggaggacg aaataagagc cgatgttgaa gctcatccgt
     6721 taaagatagt atgcggaaca gttggagaag atgagcactc tgtcggatta agagagatca
     6781 tcgacatcaa gcacggcggt attgaaagat tcggtataga agttcattat cttggaactt
     6841 cagtgcccgt ggaaaaactg gtggatgcag cggtggaact gaaagcagaa gcaattcttg
     6901 cctccacaat aatatctcat gataatatcc actataagaa tatgaagagg atacatgagt
     6961 tggctataga aaaaggaata agagatgatg taatcatcgc cgcaggagga acacaggtaa
     7021 ttcccgaaga gggactgaaa accggtgtag atgccgcatt cggcagaaac tctcacggaa
     7081 tagatgtggc ttcgttcctt atagaagaaa gacagcgtag aagaaaggac aaataaaata
     7141 ttttttcagg aatttgcggg cgggcatttg cccgtccgca atttttgttt tatccatttg
     7201 atatatccat gcatattatt gtaaaaaaaa taatatcatg tatgatataa atatcggcga
     7261 aagaaaagga gtttaaatga aaatagatgt gctggtggcg gaaataggtt caactactac
     7321 agttgtaaat gcttttgata aaatagatac aaatgacccg gtattctggg gacagggtca
     7381 ggcacccact tccgttcttg aaggagatgt gcgcgtgggt cttaaaggcg ccattgcgga
     7441 tctttgttgc agaaaaggaa tagacagtct ggaatatgac agcatgctgg ccacatcatc
     7501 tgcagcagga ggactgaaaa tgacagttca cggtcttgtc tacgatatga ctgcaagggc
     7561 ggcaaaggag gcagctctcg gtgcaggagg gataatacat tatattactg ccgggagaat
     7621 aagaaggacc gatcttaaaa agatacagga gatccgcccg aaccttatac tgatagccgg
     7681 cggggtggat ttcggagaaa gagacaccgc catatataat gcggaaatga tacggggcat
     7741 ggatatggac gtgcccgtta tttatgcggg caatgtggaa aatcacgaag aaataaaaat
     7801 gatatttgag gatgcgggca taaaactcta tatagtcgaa aacgtttatc cgaaaataga
     7861 tgaccttaat gtggaaccct gcagaaaagt catacaggat gcctttgaag aacatatcat
     7921 acatgcgccg ggtatggaac atgtaagaga aatggtagac ggccccatca tacccacacc
     7981 gggagcggtg atggaatgta caaagctcct gaatgaatat ataggcaatt tgatggtact
     8041 ggatgtgggt ggagccacca cggacgttca ctctgtaaca gaagattcgg aacagatagc
     8101 ggcacttctt atcagtcctg agcccagagc caaacgtacg gtggaggggg atctgggtct
     8161 atacgtcaac aggatgaagg tcatcgaatc aataggagaa gacaacttca gaaaagatct
     8221 tgccggcata gatgttgaaa aggcccttga aagctatgtg gctataccaa ggaacggtga
     8281 tgaagtaaag ctcgtggaga ggcttgcgga ggaagcagta ctaaaagccg ttgaaagaca
     8341 tgcaggtaca ataagatatg tatacggacc ttccggaagg cagactctgg cagaaggaaa
     8401 agatctcact caggtggaat atataatagg aacaggagga gctcttacaa gactgccgag
     8461 aaggatagag ctcatgaaga agatcccgaa acacaatgaa tcagggatga tgcttttccc
     8521 cggtgaggga gcgaagatac tggtggataa tgattatata atggcatctt taggcgtcct
     8581 ttccaaaaga tataaagaag ctgcagtaaa actgcttgaa aaaagcctgg acttcaagtt
     8641 ccccaaaaaa aaggaggcag aataatgtat ccgagattat ttataaacat agaaaaactc
     8701 aaaaaaaaca tagatgctgt agcaaaaatc acgaaaaatg atggaaactg ttccatgatg
     8761 atcgtcacaa aagccctttg cgcagacaga aaaacagctg aaatgatagg aaaacatccg
     8821 caggtggact atgtggcgga ttcacgtata tcaaacatca gaacctattc ggaattcgta
     8881 agaaaaaacg gcaaggaaac ggtacttatg cgtctgccca tggaatcgga gataaaggat
     8941 gtggtaaggt atgcggatat aagtttcaat tcggaggttt ccacccttga gatgctggat
     9001 aaagaagccg caaaacaggg ggtcgttcac aaagtcgtac ttatgataga cctgggagat
     9061 ctgagagagg gcatgttttt tgagaatgaa aatgagatat tcaaagcagc aagaaccgtt
     9121 cttgccgccg gtaatcttga gcttttcggc cttgggacaa atctcacctg ctacggagca
     9181 attataccaa aatacgataa tctttcggtg ctttgcacat gggcggacag gatagagggc
     9241 aaattgaaca taaagatccc gatgatatcc ggaggcaatt ccagttcaat ataccttata
     9301 ccaaaaggcg aaatgcccga aagaataaac aatctcagac tgggagaatc ctttcttctc
     9361 ggcaatgata cggcttactg cacaaaattg gacgggacaa cagatgatgc cctgatactg
     9421 gaggctcaga taatagaatt gaaagagaag ccttctcttc ccatcggaga agtcggtgta
     9481 gatgctttcg gtaacagacc ggaatacgaa aacagaggaa caataaaaag agccatactg
     9541 gctgtgggac agcaggatac cgaacagagc ggacttcatc ctctggacga aaaagtggat
     9601 gtcctgggag ccagctcgga tcatttgata ctggatgtta ccggatccga caaaaaatat
     9661 aaaacaggcg atgttataca gttcaaaatg gaatacgggg cagtgctgaa agcggccaca
     9721 agtccctatg ttgagaaagt atataaataa atctgaaaac ctttattcat atattcagtt
     9781 acagtaaaag caatggagga atagatatgt ttaaattcgc ttttattatc aatctgcccg
     9841 gagaatcacc gggatctttc agcaggatct atgaaacaaa agaaagtttg agcagggtac
     9901 tggcaacagg atccgatgaa atgaccagag agactgtcaa aaaactggcg gaagaaggat
     9961 atgaactggt caatttctgc ggagattttg atgatgaaat gacaaaaagg tattatgagg
    10021 ctgctgaagg cacaatagaa atgaggcatg cggactactt tccaaaagag atggaaaaac
    10081 tggaagcctc gtcatctctt aaggaatacg ggatcataat aattgcccgg gggacagagg
    10141 aaacttacaa aataaaaatg aaaagtcccg attgtaatgt aaacgtttgt tttgtaaagg
    10201 atatagattc ggcaaagacc gccgtctgcc agcttgttgc tgatggtata gacttcattg
    10261 aactttgcag ctggttcgat aaagagaaaa cagaggtagt catagaagcc atcggcggga
    10321 aagttcctgt gggaagttgc ggaaaaataa actgaccctg atttgatcag gcacatgtca
    10381 aaaatcatta tatttgacgg aagaagatct tattagttca agtttaaggg aacggctcat
    10441 attttttatg gaccgttccc ttttgagtgc ttctgatttt gtatcatatt tttcgcaata
    10501 aacaagactt acgggtctgc ggctcttggt atatttggcg cccttgccgc tgttgtgcgt
    10561 ccggaccctg ttttttatat ctgtgctcca gcccgtatat aatgtgccgt cggcacattc
    10621 caaaatgtaa acatagtgtc cagaaaactc cttatacata actcacctgt catttgcaga
    10681 acggatctgc ttatcatacg cttctttgtt tttttagcag taaggaattg gctgccataa
    10741 agacagttga aagggaaaca agtataataa ggtctatata tatttcagat aacccgtttc
    10801 cgtgcagcat tacttcagta agagcgtcgg tggtatagta aagaggcata aagtaaccgg
    10861 cgatctccca gcctcccgag agttcgaaca aaccgcagag gaatatctgt ggaagtataa
    10921 caaggggaat gaactgaacc atctggaatt cactgtttgc aagagtcgaa agaagcattc
    10981 caagcgtaag ggcgcacatg gctgtaacaa gattcacaat aagaaccagc catatacttc
    11041 cttccagggg cattttcaga acatatataa caaaaaatgt tacgatggat gactgtaaaa
    11101 cagcaagaac ggaaaatccc agagcgtagc ccgttattat ttctcctctt cttatgggag
    11161 tcgaaaggag tttctgcaat gtgccgctgg tccgttcccc cagaaaattg atgcccgcta
    11221 tcagaaagac aagaaagaaa attattatac ctatcatggg tgcaccgtaa ttgtcaaaca
    11281 gagaccagtc atcactgccg taaatgtaat ccgtttcgat ctcgggttcc gcaaatgata
    11341 cggggaccgg aatatctttt tttattgcct ctgcggcggc aactgttatc agggctttga
    11401 tcctggatgc tttagaagca tccgttccgt caattaggac ttctatatcc gtgtaatcgg
    11461 ccagatcatt ttcattgtcg ggtatataaa tcgctgcggt tatttcgccg tctttgattg
    11521 ctttttctgc aagggcgctg tcataatagg aaacttttac atcaacatcc tcattatcct
    11581 ccaaggtttc gacaaaatgg tccggagctt ttatgatccc tatatcgtat cctgaatcac
    11641 ctgagtccag tataaaataa acgatggtca gtatcaaaag tggagcaaca acgataatgc
    11701 ccaccgtgcg cttgtcatgg cgtatctgat ttagtatacg cagtgcaacc gctttaatcc
    11761 tcatcagaac cacctcctct gccaaagtat ataaatgcat cttcgatggt tttggctcct
    11821 gacttttcta ttatttctga gggtgttcct tcggccagga aaacaccttc ccgcatcata
    11881 gccagcctgt ggcactttcc cgcttcatcc attacatggg tggtaacaag tatagtgaca
    11941 ccctggtcgg atagatagta gagttctttc catatacttt ttctgagcac cgggtctatt
    12001 cccactgtgg gttcgtccag caaaaggact tttggatccg gaaggagagc tatggccaga
    12061 gagagtctct gtttcatgcc ttctgaatat gcggaaacag ttttcttgag ttcatgtgta
    12121 aggcctacga gatccgtcac tttgtctatc ctttcaacca aaacatgacc tttgaatcca
    12181 tatacacttc cgaaaaattt gagattttca taggcggaaa gggtgggata aagggctgcg
    12241 gactgggcca tatagcccat ttgagccata agagacagat cgggcatgag tcctcccatg
    12301 atatatgcac tgccggagtc ctgtttgagt attcccgcca tgattttgac agaagtggtc
    12361 ttcccgcagc cggaaggacc taaaagcccg aaaatacagc cctttgggat tttcagcgaa
    12421 acatcgaaaa gtacctgttt tttgccgaag cttttgttta tattatcaag tctgatacag
    12481 ttttccataa tatatcatcc tttcatacag aaaagatttc tgcttcttgc aacatataat
    12541 atgttattat atcatataaa agaaaagcat ttatagaaat caaaaaaagc gtttgatgat
    12601 attaaaaggg agaaaaacat gtcaagactt gaaaaaataa aaacaggact gcccggactg
    12661 gacgggatac ttcagaatgt gagacagggg gaaagcgtgg tatggcagat ctcggaagga
    12721 gaagattatg cctattttgc caggcatttt gcagcccagg ccataaagga agggcgtaat
    12781 gtcatatact tccggttttc ggactatcct gcgctacttg agaagcagga gggattaaag
    12841 ataatcagaa tggatctcaa ctccgggttc gaaaaattta ctgtaagtat atataaagtg
    12901 atctcaaaac aggatcccgg tacattttat gtttttgaca gtatgtcaca gcttcagacc
    12961 gtatgggcag cggactttat gatgcgtaac ttcttcaaag caatttgccc ggcactgaag
    13021 gaaatgaaag ggacgggata tttcagtata gcctataaag ggcattccta cgattcaata
    13081 agtcagataa aggaaacagc tgatatatat ataaatactg tgagcggtcc cgaaggaata
    13141 tatgtgcagc ctcttaaggc tgcaaacagg aagtcgccga caatgttttt cccccacctg
    13201 atatcggatg aaaaggcggc aaaacttccc cccattacag atggtataag cagcagcaaa
    13261 tattacggac tgttaaaaat aaaggccagg aacccggggc agagattcct cgacaactgg
    13321 gacgttttcc ttatggaagc acagacggcc atgcttgaag aatctccgga tatggaactt
    13381 tatgaaaaga aactttacaa gatgcttata ggcagagacc aggaaagagc caatcttttc
    13441 aaagccaatt ataatataca ggattatctt aacatcaacc tgagactggt aggaaccggt
    13501 agtataggag gcaaagcggc aggaatgctg ctggcccgta aaataataga aaacaataga
    13561 ccggatctgg cggagcacat agaagaacac gattcgtttt atgtgggctc caatgtattc
    13621 tatacatttc ttatcagaaa taactggtgg aaactctggc ttgaacacaa aagcgatgaa
    13681 ggttatttta tggccgccag agtattgaaa agccagatac cctacggcga ttttcccgat
    13741 cttgtcaaag aaaaattcag aagactgata gatcattacg gacagaatcc tataatagta
    13801 agatccagtt ccctgctgga agacggattc ggaaatgcat tcgcgggcaa atacgattcg
    13861 attttctgtg tgaacatcgg ctctcccgaa gaacgttatg aacagtttgt aaaagcggta
    13921 aaggatgttt atgccagtgc catggatgaa tccgcattga tatacagaaa acagagagga
    13981 cttgacaagg ccgatgaaca gatgtctatt ctggtgcaga gggtctccgg gtccgtattt
    14041 caagatgttt ttatgccggg agccgcaggt gtgggctatt ccatgaactc atacacatgg
    14101 aacaaatcca ttgatccaaa cgagggaatg atacgtcttg ttgctggact gggaaccagg
    14161 gccgtggaca gaactttcca ggattatccc cggcttgcag ctcttgataa gccggcactc
    14221 aagccatcaa tgctgccgga agacagattc aggttcgtcc agaaaaatgt agatgtattg
    14281 gactttacag agaacacact tcttactata ccccttgaag aggtggcgga aaaagcaccc
    14341 aactggtata attcgctttt tgttgagcat gactatgaga ctgaaaacag actgagtgat
    14401 atgggacgca gacagcaggt gatctccacc acatgcgaaa aaatgctcaa gaatgaagag
    14461 ctggtgtcca ccatgagaga agtattaaaa accctgcatg aacattacaa atatcctgtg
    14521 gatatagaat tcacgataaa cttttccgaa aagggagagt tcctgataaa tcttctgcag
    14581 tgcagaccct tgcagtcaaa gggcaccggt atagcaggtg tgaaaatacc ggatgtgcct
    14641 gaagaaaaaa catttatgaa attgttcaag aacaccatgg gcggccccac aaaaatggag
    14701 tttgacatgg tagtgatagt cgatgccaaa ggttatgcgg aaatgcccta taaagaaaag
    14761 ttctctgtgg caaatgccat acatgcggtc aatacttatg cgggacaaaa caaaaaaagc
    14821 cttatgatac tgggaccggg cagatgggga actaattcgg cagaactggg tataccggta
    14881 agatatgccc agataagcaa tgtgaacgca atatttgaaa tgtccttcga aagtagcggg
    14941 cttatgccgg agctttcttt cgggagccat ttcttccagg atcttgtgga gaccaacact
    15001 ttttacgggg ctgtttttga aaaagacagt tccgaaggag taaaaagcat atacaggccc
    15061 caggtacttg aaaacgaaaa agaagtatat gatgagatac cggataccat caaagccctt
    15121 aggaacataa taaaagtata tgaactggaa gaaagcaaaa tgatcctggt ggcagacagt
    15181 aaaggtgaag aaaccgtttg ctggaaagaa aaagaaaaga cgaaaaacag caaataaaaa
    15241 gaagccccta tgccaaaagg gctgcagaga tccttaacag gaacttaaca tatttaacag
    15301 aaacataacg gtattattaa tactcccgta acaatacggg ggtattattt acttgtaaac
    15361 aaaagtaaaa aaatacggcg gcgaaaaagg atccgccgaa gaatgcaagg gcattcagga
    15421 aggaaagaaa aaatgaaaaa gaacaaaatc aaaaaaatca ttgcactggg actggcaatg
    15481 gcactgacaa caggagtcat gtcggcctgc ggaggagaaa gcggaagcga tgagcagaaa
    15541 agttccggag aaactgtagt tatagcggga tctacatcag tacagcccct ttcggaggtg
    15601 atggcagaag agttcatggc agataacgaa ggtacgtcag tagaggtcca gggcggaggc
    15661 tcgggacagg gcataaaggc catcgaagag gggatagccg acatcggatc tctttccaga
    15721 gaagtaaagg atgaagaaaa aggttctgta aaagaagagt acataatagc aatggacggc
    15781 gtggcagtag tagtcaatac agaaaccgaa acggaagata tcaccattga agaacttgaa
    15841 aaggtctata ccggtgagat cacaaactgg aaagagcttg gcggaaaaga tgcggaaata
    15901 acggtagttt ccagagaaga aggctccggt accagaggag ctttcacgga aata
//
LOCUS       DAONHK010000008        15510 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_2994_length_15510_cov_4.707409, whole genome shotgun sequence.
ACCESSION   DAONHK010000008 DAONHK010000000
VERSION     DAONHK010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 15510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15510
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2994_length_15510_cov_4.707409"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(101..949)
                     /locus_tag="PLM92_00700"
     CDS             complement(101..949)
                     /locus_tag="PLM92_00700"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008427158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HPR23697.1"
                     /translation="MKKIGVLGTGTMGAGIIQVMAQNGYEVVLRARRQTSVDGGMAKV
                     KKNLEKMVAKEKITEKDKEEILGRITGSTDISIVKDADLIIEAATENMEAKKALFAEL
                     DELCKPDTIIATNTSSLSITEIAAATKRPDKVIGMHFFNPVPVMKLVEIIKGLATSEE
                     TKDVILKLTESIKKTPVEVAEAPGFVVNRILIPMINEAVGILAEGIADAEGIDNAMKL
                     GANHPMGPLALGDLIGLDVCLAIMDVLYEEFGDPKYRAHPLLRKMVRGNQLGMKTGKG
                     FFDYSK"
     gene            complement(1087..2322)
                     /locus_tag="PLM92_00705"
     CDS             complement(1087..2322)
                     /locus_tag="PLM92_00705"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF010697.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HPR23698.1"
                     /translation="MDIKIDIEKSGVSLAEIKSFDEAAEGAMDMLWSGKKEFTGWVRL
                     PLEKNSEALEEILMTAAEIQAKCELFVVIGIGGSFLGAKALIDSLAEEQPGLPHVIFA
                     GNNLSGTYHSSIVSEVRKKETCICVISKSGSTTEPLAAFSVLKEIMKEKYGKKTSGRI
                     YAITDEKKGQLRKEVHKEGYESFIVPADIGGRYSVLSTVGLLPAAVAGVDIKSLLKGA
                     EIMAKDPAWDLDATNYAATRNALMKKSKKIEIFEFFEPSLEYFGEWLKQLFGESEGKE
                     GKGLFPASLSFTRDLHSVGQFLQEGTPVFFETMIMTGCNNRDIVIPDSAGDPLAGKTL
                     NEINQCVAKGVIKAHEKAGVPMIIIEMPETDAYYMGQMIYFFELSCAVSSYINGVDPF
                     DQPGVERYKEEARREILEL"
     gene            complement(2348..3865)
                     /locus_tag="PLM92_00710"
     CDS             complement(2348..3865)
                     /locus_tag="PLM92_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009643271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HPR23699.1"
                     /translation="MKETKGFDADKYIEEQSKYILRRIEEGNSERLYLEFGGKLIQDR
                     HAMRVLPGFDENAKIKLLNRLREKTEVIICIYAGDISTSKTRQDFGITYDLEVLRLID
                     MFRKYELEINSVVVTRYEDVPAVNMFINKLSRRGIKTYKHYFTKGYPTDVETIVSDEG
                     YGANPYIEVTKPLAVITGPGGGSGKLATCLSQLYHEYRRGKKVRYAKFETFPIWNLPL
                     KHPVNVAYEAATADLKDVNMIDSFHLEAYGKTTVNYNRDLEVFPVVKRIIEKITGEES
                     EYRSPTDMGVNRAGFSIINDEICKEASRQEIIRRFLIAQCDYKKGKINEDAVKRSKLL
                     MDEMNLKIEDRKVVIPARDYAEKKRNEDPKYENVVVMALELPGGRIVTGRSSRRMVAA
                     AAAILNSIKILSGLADEIHLLSPHVLTGIQELKVNILRQERSSLNAEEILSALTVSAA
                     NNPSAAAAASKLKELSGCRAHCSAILSDRDEQTLKSLGIDTTCDPEYVTTNLYYG"
     gene            complement(3992..4447)
                     /locus_tag="PLM92_00715"
     CDS             complement(3992..4447)
                     /locus_tag="PLM92_00715"
                     /inference="COORDINATES: protein motif:HMM:NF020711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HPR23700.1"
                     /translation="MKRISRKGIEMKNVTLKITGKHIYENVEEDQMELVTEGKLDKKG
                     DTLYLTYEESEFSGTPGCRTELILTQDVISMSRSGLSVGLDTEIRFKKGERHKGFYDT
                     PFGPVEMEVLTNSIENSITYDKKGRVDIDYSISLKGLSEGRSKLNIEVM"
     gene            complement(4444..6177)
                     /locus_tag="PLM92_00720"
     CDS             complement(4444..6177)
                     /locus_tag="PLM92_00720"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HPR23701.1"
                     /translation="MDHKSEYKKWRHNRELPEDIKKELELMEGNDEEIYEAFCRDLEF
                     GTAGIRGIMGAGTNRINTPVIKRVTQGICDYMNQNFKDPAVALCYDSRINSRLFAEET
                     AAVLTANRIKAYLYKQLMPVSALSFAVREIPCNMGIMITASHNPKEYNGYKVYNSQGC
                     QILNEVPGEILSFIKRTDIFKDVKSMIFEEALKKGCEFISEDIEEAYIKKAFSYSIPQ
                     DMKGFKLVYTPLNGAGKEPVKKLLSMAGLDSINIVKEQEDPDGCFTTCPYPNPEIPEV
                     YDLAAKLCRELKADLIMATDPDCDRVGVAVPKGDGYEVLSGNQIGTLLFDYICKNKKL
                     PRAPVAVRTIVSTSIIDHMAAKKEIEIKETLIGFKYIGEQIRILENMGQEERYVFGFE
                     EGNGYLAGSYVRDKDGVTTAMLICQMAAWHKERGRDLTEALEEIYDEYGCYREKVLNF
                     TFEGTKGSKKMKKIMDFLRKNQRNGLFDKKPIVITDYLKGIKHIIGDSKACSMVAGEK
                     PTELPKENIMEFRFSDESKLIARPSGTEPKLKVYLFARARDVQSAEKILSEMEKNIDA
                     LICKQVKGYNN"
     gene            6315..7505
                     /locus_tag="PLM92_00725"
     CDS             6315..7505
                     /locus_tag="PLM92_00725"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HPR23702.1"
                     /translation="MKDQKTPQGELFENSETLSEKGNRKSGKKRSKPVDLTRKSLAVI
                     MAVCILLAGLMGFGGAMVAENLLENGSPGTSVSSSGFDLEDATGSEMTVQEISENVSD
                     SVVEIRTESVSTDLWIKEYVTEGAGSGVIVKNNGYIVTNNHVAEGASSIYVTLKNGEE
                     YKAELIGKDSLSDLAVLKIDAKNLNAVTMGKSDQLDVGDLAVIIGNPLGELGGTVSAG
                     IISALDREVTIDNTPMSLIQTDASVNPGNSGGGMFNQYGQLVGIVVAKSSGSDVEGLG
                     FAIPINSAASIIDDLIKNGRVEGRANTGMSYVEISDEDQAIQYGLSKAGVYIQQVNGN
                     NAKNAGFRSGDRVVRADGKKIDSFITLKSIIMSHKAGDKITYIIERDSSTLDITFTLS
                     ESNS"
     gene            complement(7531..8196)
                     /locus_tag="PLM92_00730"
     CDS             complement(7531..8196)
                     /locus_tag="PLM92_00730"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HPR23703.1"
                     /translation="MLNFEIQNHRPLREIVYEELKMQILKGQIEPGTRMMEVEMAEEM
                     GVSRTPIREAIRKLEKEGLVTIEPRRGAYASQISTKDIVDILEVRQNMEGLAAFYAAL
                     RMSYQQKEELKKVSEAYNAAVAAGSTPDMIKYDTVFHHLIVEGSGNKILVHMVEQLQE
                     LVLRFRYIYYDDFRRAEKMPAEHKEIFDAIKSGDTERARTAADVHIDRLKEMIEDEKV
                     LTE"
     gene            complement(8209..9177)
                     /gene="ispE"
                     /locus_tag="PLM92_00735"
     CDS             complement(8209..9177)
                     /gene="ispE"
                     /locus_tag="PLM92_00735"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HPR23704.1"
                     /translation="MKEVTINSYGKINLSIDITGKREDGYHRVEMIMHQIMLHDDVTL
                     CWKSSGIPGPVNDQNKKAGCGIKINVSSDKKYVPAGEDNIAFKAAALMAKHHGRGLLG
                     EVDIFIKKRIPVAAGLAGGSGNAAAVLHGLNVLWGLSLGTKELCILGESLGADVPFAV
                     LGQARCSPAVDEKTRKDPMASSCAFAYGRGTLLRPVTPLKSFVVISKPPISISTGEVY
                     SGMDKREIEVHPATKELISALDEGNHKKVAVNMINVLEIYTLSMYDEVVYTKNKMLEL
                     CKDEPVMMSGSGPTVYALIQQKNKAKDVYNKMKEYNRETFLTETTL"
     gene            complement(9230..10069)
                     /locus_tag="PLM92_00740"
     CDS             complement(9230..10069)
                     /locus_tag="PLM92_00740"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HPR23705.1"
                     /translation="MKRAGVLISLSLLIIMLSTAFASAAPFGVEETSPKNGETGMSVD
                     NMGVKVYFNEDIYSKDNQKANAKRCKLTDPKGKKVPVRTVFNPKEKNIMMVLADTEKG
                     AKIKGSTKYKLTIEAGFVSSKGNIMEKPVTVSFKTLNPQSSMTVSMVMMGIMVVGMIF
                     FTSREAKKQAQKDVRKKEEAVNPYKEAKRTGKSVEEIVAAEQKKKEKQAAKDEAKKKR
                     HKEENKVEISSDNIRVSKPMPISLAGSTYRTGRAKAARIAEEKEKQKASQKSKKKSKK
                     KKH"
     gene            complement(10066..10521)
                     /gene="tadA"
                     /locus_tag="PLM92_00745"
     CDS             complement(10066..10521)
                     /gene="tadA"
                     /locus_tag="PLM92_00745"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018672360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HPR23706.1"
                     /translation="MQEALIEARRAFDLGEVPVGAVIVKEGRIIGRGHNLTESSKDPT
                     AHAEIIAIRQATENLGGWRLTGCDIYVTMEPCSMCSGAIVLARLKKLYIGTMDKKAGA
                     CGSVLNIINDSRLNHYTETETGIMQEECESMMKEFFKKLRTKKSEEDKT"
     gene            10626..11363
                     /gene="srtB"
                     /locus_tag="PLM92_00750"
     CDS             10626..11363
                     /gene="srtB"
                     /locus_tag="PLM92_00750"
                     /EC_number="3.4.22.71"
                     /inference="COORDINATES: protein motif:HMM:TIGR03064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0016485 - protein processing [Evidence
                     IEA];
                     GO_process: GO:0045184 - establishment of protein
                     localization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HPR23707.1"
                     /translation="MTKEKAKKNKISLVGKIVFVISLVLLVISLGILIDHYMKGQKSE
                     KEFSSLKVSGTYDLDALYRQNSDIIGWIKIEDTNVDYPVMQTRSQPEFYLRRNFKKEY
                     SLSGTPFMDAASDITKPTKNWLIYGHNMKNGTMFHDILNYEDAGFYEKHKIFTFDTIE
                     ENGKWEVVAVFRSKIYKKNSGKFKYYEYGGIVSGEQLKEYVGICKSLSQYDTGVEVNE
                     DDTVLTLSTCAYHTKDGRFAVVAKKTE"
     gene            complement(11417..12058)
                     /locus_tag="PLM92_00755"
     CDS             complement(11417..12058)
                     /locus_tag="PLM92_00755"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HPR23708.1"
                     /translation="MVLDIIVAVILIGTTVYGLRKGFVFTFIHTVGWILAAVLAYLAT
                     PFVKEFLVEKTTVYEAIEEDLNLRFSDSVPVMETSEKSVPLNISDILQNSAEIVSSKM
                     AEIFAGLVFTVLVFIALLAFLKIILWLILRLLSRDYRDGFAGFADGFFGLIVGFIKGA
                     VIVFIFLALLLPVTNLINPDSSEMIVETLENSYFAGELYDNNFIMVLLEGFLF"
     gene            12459..13547
                     /locus_tag="PLM92_00760"
     CDS             12459..13547
                     /locus_tag="PLM92_00760"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPR23709.1"
                     /translation="MGYAAPSIHGLCPYGGLASSLSIIAYGTFFKKIMIGTFIIFIST
                     MSLAIVFRRSFCGQICAFGALQEFLGNLGRSIFKGRRFVITTKIDRIFRYLKYLVLIF
                     TVAMSWMTGILWITPYCPYNALGHMADFQSLTSIYLIGFILLIITLIGSFFYERFFCK
                     YLCPAGAFYAIIGKISPFAVRINKDKCIDCGLCNKICPMNVNIMNSKSEKITDSECIN
                     CNECVNTCPKKGALNAGFSKKTVLHPILATLLVLALFFTPIIAAKSTDSFQILPNKFG
                     SGSGIHENGKDKGLNDKGQSKIGNVYMKDITGSMTMSEISSLLDLSIKNLYIKIGLPE
                     NYPENNTIKQAAESLGSTVSDLKNKIFE"
     gene            complement(13635..13710)
                     /locus_tag="PLM92_00765"
     tRNA            complement(13635..13710)
                     /locus_tag="PLM92_00765"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13675..13677),aa:Arg,seq:ccg)
     gene            complement(13770..13845)
                     /locus_tag="PLM92_00770"
     tRNA            complement(13770..13845)
                     /locus_tag="PLM92_00770"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13810..13812),aa:Arg,seq:cct)
     gene            14123..14989
                     /locus_tag="PLM92_00775"
     CDS             14123..14989
                     /locus_tag="PLM92_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005838126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPR23710.1"
                     /translation="MTTKQQGILLMCTSALLFSAMQIAIKSSADDIPLMEQVFFRNIV
                     SLVISFIIIKKNNGSLFGSKEHQPLLFVRSVFGFLGLMAMFYAAGHANQGDVSTLIKL
                     SPFLITLWAAIFLKEKIKKIQVPALILAFTGAIFVANPAWTSNIFPLFTALLCAFFSS
                     VSYTLLAYFKNKVDGMTIIMHFSTFCVLCSLPFMLMDFVMPGTYQLLQLLLIGFLGGF
                     GQIALTYSYRMAPAGEISIYNYSGIIFSIILGFLFLGERLAWNSWIGIFFVLLASFLV
                     YRFSGSDVDTSL"
     gene            15025..>15510
                     /locus_tag="PLM92_00780"
     CDS             15025..>15510
                     /locus_tag="PLM92_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HPR23711.1"
                     /translation="MKNYNSPFSQRYSSNEMKYLFSSENKFRTWRRLWVALAEAEKEL
                     GIKITGAQIDELRSNITNINYAAAEKKEKEVRHDVMAHVYAYGLQCPSAKGIIHLGAT
                     SCYVTDNTDLIIMKDALLVVKKKLINVLSALSGFANKYKSLPTLGYTHFQPAQPTTVG
                     KR"
BASE COUNT         3941 a         3554 c         2975 g         5040 t
ORIGIN      
        1 gtggttttta tctcgtgcgc ccgcgcactc gacacgggct taaacgccct attaaataaa
       61 aggggctgcg cattacacac agccccgctt ttgtaaaaca ttatttgctg taatcgaaaa
      121 aaccttttcc ggttttcatt cccagctgat tgcctcttac catttttctg agaagaggat
      181 gtgctctgta tttgggatca ccgaattctt catatagtac atccatgata gcgagacata
      241 catcgaggcc tataagatct cccaatgcca gcggtcccat ggggtggttc gcaccaagct
      301 tcatggcatt gtctatgccc tctgcatctg caataccttc ggccaaaatt cccaccgctt
      361 catttatcat gggtatcagt attctgttta ccacaaatcc cggtgcttct gccacttcaa
      421 cgggtgtctt ttttatcgat tctgtaagtt taaggatcac atcctttgtt tcttcagatg
      481 ttgcaagtcc ctttattatt tcaaccagtt tcataacggg aacaggattg aagaagtgca
      541 taccgataac cttgtcgggc cttttggtgg ctgccgcaat ttctgtgatg gaaagggaag
      601 acgtattggt ggctattatg gtgtccggct tgcagagttc atccagttcc gcaaacaggg
      661 ctttttttgc ttccatgttt tctgtagccg cttcgattat cagatctgca tccttaacga
      721 tacttatgtc cgtggatccg gtgatcctgc cgagtatttc ttctttgtcc ttttccgtga
      781 tcttttcttt tgcgaccatt ttttcaaggt tcttctttac tttggccatg cccccgtcaa
      841 ctgatgtctg tcttcttgct cttaagacca cctcatagcc gttctgtgcc attacctgta
      901 tgatccccgc tcccatggta cctgtgccta atactccgat ttttttcatt taaatatcct
      961 ccttaacgat atgcttttga tattgttaaa aaattaacta tacttaatta gtattatacc
     1021 attgcttatt tttttgtaaa tactgatctg tctttttgaa tatgttcatt ttttctgata
     1081 attcctctaa agttcaagta tttccctgcg ggcttcttct ttatatcttt ctaccccggg
     1141 ctggtcgaag ggatccacac cgtttatata cgaagagaca gcgcaggaaa gctcaaagaa
     1201 atatatcatc tgccccatat aataggcatc cgtttcaggc atttctatta ttatcatggg
     1261 gacaccggct ttttcatggg cttttatcac ccctttggcg acacattggt ttatttcgtt
     1321 aagggttttt cctgccaggg ggtcccctgc ggaatcgggt ataacaatgt ctctgttatt
     1381 acaccctgtc attatcattg tttcaaaaaa cacaggcgtc ccttcctgaa ggaactggcc
     1441 cacagaatga agatctctgg tgaagctcag actggcgggg aaaaggcctt tgccctcttt
     1501 tccctcgctt tctccgaaaa gctgcttaag ccactctccg aaatactcca gtgacggctc
     1561 aaaaaattca aatatctcta tttttttgct cttcttcatc agagcatttc ttgtcgctgc
     1621 ataatttgtt gcatcaagat cccaggcggg atcctttgcc atgatttctg ctcccttaag
     1681 gagactcttt atatctactc ccgcaacggc tgccggcagc agtcccaccg tgctcagaac
     1741 ggaatatctt cctcctatgt cggccggcac aataaagctt tcatatcctt ctttatgtac
     1801 ttcttttcta agctgccctt ttttttcatc tgttatggca tatatcctgc cggaggtttt
     1861 tttgccgtat ttttctttca ttatttcttt aagcacgctg aatgccgcca gaggttctgt
     1921 ggtgcttccg gattttgata tgacacatat acaggtttct ttttttctta cttcacttac
     1981 tatgctgctg tggtaagttc cgctcaagtt gttgcccgca aaaataacat gtggcagacc
     2041 tggctgttct tctgccagag aatcgatcag ggccttggct cccagaaagg atccgcctat
     2101 gcctattact acaaaaagct cacactttgc ctgtatctcc gccgctgtca taagtatctc
     2161 ttccagcgct tcagagtttt tttcgagggg aagtctgacc cagccggtaa attctttttt
     2221 gccggaccaa agcatatcca ttgctccttc ggcggcctca tcaaaggatt ttatctcagc
     2281 aagggagaca cctgattttt ctatgtcaat ttttatatcc attttacttc tcctgccgta
     2341 gatattttta accgtaatac aggttagtgg tcacatactc cggatcacag gtcgtgtcaa
     2401 tacccagaga ttttaaggtc tgttcgtcac gatcacttaa aattgcactg cagtgggccc
     2461 tgcatccgct cagttcttta agctttgatg cggctgccgc tgcagaaggg ttgttggctg
     2521 ccgacactgt aagggcgctc aatatttctt ctgcattgag actggatctt tcctgcctga
     2581 gtatattcac cttcagctcc tgtattcccg taagcacatg gggggacagc agatgtatct
     2641 catctgccag tccggaaagt atttttatac tgtttaatat tgctgctgct gctgccacca
     2701 tccttcttga gcttctgccc gttactattc ttcctcccgg aagttcaaga gccatgacca
     2761 ccacattctc gtatttcgga tcttcgtttc tctttttctc ggcatagtcc cttgcaggta
     2821 tgaccacttt tctgtcttct attttaagat tcatttcatc catcagaagc ttggatcttt
     2881 ttactgcatc ttcatttatt ttaccttttt tgtaatcaca ctgggctatc agaaatctcc
     2941 tgattatctc ctgccttgaa gcttctttgc atatttcatc atttataata ctaaatcccg
     3001 ctctgttgac gcccatatca gtgggtgatc tgtattccga ttcttcgccg gtgatttttt
     3061 ctattatcct ctttacaaca ggaaaaactt ccagatcccg gttgtagttc acagttgtct
     3121 tgccgtatgc ctccagatga aatgaatcga tcatgttcac atctttcaga tccgcagtgg
     3181 cggcttcata ggcaacattg acaggatgct tgagcggaag gttccatatg ggaaatgttt
     3241 cgaatttagc atagcggact tttttgcccc gtctgtattc gtgataaagc tgggaaaggc
     3301 atgtggccaa ttttccgctg ccgccgccgg gtcctgttat gaccgccagg ggttttgtca
     3361 cttctatgta gggattggct ccatagcctt catcacttac aatggtctcc acatctgtgg
     3421 ggtagccttt tgtaaaataa tgcttgtagg ttttgatgcc tcttctgctg agtttgttga
     3481 tgaacatatt tactgccggc acatcttcgt atcttgtgac aacaacactg tttatctcaa
     3541 gctcgtattt acggaacata tcgatcaggc gaaggacttc aagatcatat gttattccaa
     3601 aatcttgtct ggttttgctg gtggaaatgt ctcccgcata aatacatatt atgacttctg
     3661 ttttttcccg cagtctgttc aaaagtttta ttttggcgtt ttcatcaaag cccgggagaa
     3721 ctctcattgc atgtctgtcc tggatgagtt ttcctccgaa ttccagataa agcctttcgc
     3781 tgttgccttc ttcgatccgc cgaagtatat attttgactg ctcttctatg tacttatctg
     3841 catcaaagcc ctttgtctct ttcaccttcg ttctccttta aatgataccg ccaactaata
     3901 tgagtgaagt tacgaccagc gcggccacaa ggacgattgc cactgctctt acccattttt
     3961 tgtttgtgtg catttctgtc tccttattat gctacattac ttctatattg agtttgcttc
     4021 ttccttcgct cagcccttta aggctgatgc tgtaatctat atcaacacga ccttttttgt
     4081 cgtaggtaat actgttttcg atgctgttgg tcagtacttc catttctacc ggaccaaagg
     4141 gcgtatcata aaagcccttg tgtctttctc cttttttgaa tcgtatctcc gtatccaggc
     4201 ccacactaag accgcttctg ctcatgctta ttacatcctg tgtaagaatg agttcagttc
     4261 tgcagcccgg tgttcccgaa aattcgcttt cttcgtacgt caaataaagg gtatcacctt
     4321 ttttgtcaag cttgccttcg gtaaccagtt ccatctgatc ttcctcgaca ttttcgtaaa
     4381 tatgcttccc tgttattttt aacgttacgt ttttcatctc tattcctttt ctgcttattc
     4441 ttttcaatta ttatacccct ttacttgttt gcaaatcaag gcatctatat tcttttccat
     4501 ttccgaaaga attttttctg cagactgcac atcccgggcc cttgcaaaga gatacacctt
     4561 gagtttcggc tctgttcctg aaggtcttgc aatgagtttg ctctcgtccg aaaaccgaaa
     4621 ttccattatg ttttctttgg gaagttccgt gggtttttct ccggccacca tggaacaggc
     4681 tttggaatca ccgataatat gttttatacc tttcaaataa tcggttataa caatgggttt
     4741 tttatcaaat aaaccgtttc tttgattttt cctcaaaaaa tccattatct ttttcatttt
     4801 tttactgcct tttgtccctt caaaagtgaa gttcaggact ttctccctgt agcatccgta
     4861 ttcatcatat atttcttcaa gggcctctgt cagatccctg ccgcgctctt tgtgccatgc
     4921 cgccatctga catatgagca tggccgtcgt cacaccgtct ttatctctta catagcttcc
     4981 ggccagatac ccgtttccct cttcaaaccc gaaaacatat ctttcttcct gacccatatt
     5041 ttccagtatc ctgatctgtt ctcctatata tttgaaccct ataagagttt cttttatttc
     5101 tatttctttt tttgctgcca tatggtctat tatggatgta cttactatag ttctgacggc
     5161 aacgggtgcc cggggaagtt ttttgttttt gcagatataa tcaaaaagca gggtgcctat
     5221 ctgattaccg gaaaggactt cataaccgtc tcctttcgga actgcaacac ctactctgtc
     5281 gcagtcagga tccgtagcca ttataaggtc tgcctttaat tctctgcaga gcttcgctgc
     5341 cagatcatat acttcaggta tctcgggatt cggatagggg catgtggtga aacagccatc
     5401 cggatcctcc tgttccttta ctatgtttat gctgtcaaga cctgccatgg aaagcagctt
     5461 ttttacaggt tccttcccgg ctccgtttaa gggggtatat acaagtttga atcctttcat
     5521 atcctggggg atggagtaac tgaaggcttt ttttatatat gcttcttcta tatcctccga
     5581 tatgaattca catccttttt tcagtgcctc ttcaaatatc atacttttta catctttgaa
     5641 tatgtctgtt ctttttataa aggaaagtat ttcgcccggt acttcattga gtatctggca
     5701 tccctgactg ttataaactt tgtatccgtt gtattctttt ggattgtgac tggcggttat
     5761 cataatgccc atattacacg gtatctccct taccgcaaag gacagtgccg agaccggcat
     5821 aagctgctta tataaataag cctttattct gttggctgta agtactgccg ctgtttcttc
     5881 cgcaaagagc ctggaattta tcctgctgtc ataacacaaa gctacagccg ggtctttaaa
     5941 attttgattc atgtaatcgc aaatgccctg ggtcaccctt tttattacag gagtatttat
     6001 cctgttggtg cccgcaccca ttattcctct tatcccggcc gttccgaatt caagatccct
     6061 gcagaatgct tcatatatct cttcatcatt gccttccatg agttcaagct ctttttttat
     6121 atcttccggc aactccctgt tatgccgcca ttttttatat tcagacttat gatccacaga
     6181 atttttctcc ttaatcttca caatatgtta tataatttta acataaacaa ggctaaatat
     6241 atacatgttt tccacacaat aacgccacac aaaggttgta tcgtactcat aagagaagaa
     6301 aggagggggc gctaatgaaa gatcaaaaaa cgcctcaggg ggagcttttt gaaaacagcg
     6361 agaccctttc agaaaaggga aatagaaagt ccggcaaaaa gagaagcaaa cctgttgatc
     6421 tcaccagaaa aagtctggca gtcattatgg cagtgtgcat actgctggca ggtctcatgg
     6481 gtttcggagg ggctatggtg gcagaaaatc tgcttgaaaa cggttcaccg ggaacttctg
     6541 tttcctcttc aggttttgat ctggaggatg ccaccggcag cgaaatgact gttcaggaga
     6601 tctcagaaaa tgtttccgat tctgttgtgg aaatacgtac tgaaagcgtc agcacagact
     6661 tatggataaa ggagtatgtc acagaaggag ccggaagcgg agtaatagta aaaaacaacg
     6721 gatacattgt cacgaacaac catgttgccg aaggtgcaag ttcaatatat gtgaccctga
     6781 aaaacggtga ggaatataaa gctgaactta ttggcaaaga ctctctttca gacctggcag
     6841 ttctcaaaat cgacgccaaa aatctgaatg ctgtaacaat gggcaaaagc gatcagcttg
     6901 atgtgggaga tcttgctgtc ataataggaa atcctctggg cgaactggga ggcaccgttt
     6961 ctgcggggat aataagcgcc cttgacaggg aagtaaccat agataacact cccatgagtc
     7021 ttatacaaac cgatgcttct gtcaatcccg gaaattcagg aggcggcatg ttcaaccagt
     7081 atggccagct ggtggggata gtggtggcca aatcatccgg ttccgacgtg gaaggtctgg
     7141 gctttgccat tcccataaac tctgcggcaa gtattattga tgaccttata aaaaacggca
     7201 gggtagaagg cagggcaaat acaggcatga gctatgtaga aatatcagat gaagatcaag
     7261 ccatacagta cggactttcc aaagccggtg tatatataca gcaagtaaac ggtaacaatg
     7321 ccaaaaatgc cggcttccgc agcggtgaca gagtcgtgcg ggccgacggc aaaaaaatag
     7381 acagtttcat aactttaaaa tccataataa tgtcccataa agccggggat aaaataacct
     7441 acatcatcga aagggacagt tctactttgg atattacttt cactctttct gaaagtaact
     7501 cataacctct caaagacgaa aaagtgctga tcattcggtc agcacttttt cgtcttctat
     7561 catttctttt aatctgtcta tatgcacatc ggcggcggtc ctggctcttt cggtatctcc
     7621 gctttttatt gcatcaaata tttctttgtg ttcagcaggc attttttctg ctctcctgaa
     7681 atcgtcataa tagatatatc tgaaacgcag taccagttcc tgtaactgct ctaccatatg
     7741 tacaaggatc ttgtttccgc tgccttcaac tataagatgg tgaaaaaccg tatcgtactt
     7801 gatcatgtca ggagtgcttc ccgccgccac tgctgcatta taagcctctg aaactttctt
     7861 gagctcctct ttctgctgat aactcatgcg aagtgccgca taaaaagctg ccaatccttc
     7921 catgttctgt cttacttcga gtatgtccac gatgtccttt gtggatatct gtgaagcata
     7981 ggcgcctctt cttggttcta tggtcacaag cccctctttt tcaagttttc ttatggcctc
     8041 ccgtatgggc gttctgctga ctcccatttc ctcggccatc tcaacttcca tcatcctggt
     8101 gccgggttcg atctgtccct tcaatatctg catcttaagc tcttcatata ctatttctct
     8161 caagggcctg tgattctgta tttcaaaatt cagcatcttg tctccttttt acagggtggt
     8221 ttcagtaagg aaagtctccc tgttgtattc cttcatttta ttgtatacat cttttgcctt
     8281 atttttctgc tgtataagag cgtagactgt ggggccgctt ccactcatca tcaccggctc
     8341 atctttgcaa agttccagca tcttgttctt tgtatacaca acctcatcat acatgctgag
     8401 cgtatagatt tcaagtacat tgatcatgtt caccgccact tttttatgat ttccttcatc
     8461 aagagccgaa ataagctctt ttgtcgccgg atgtacctct atttctcttt tgtccatgcc
     8521 actgtaaact tctccggtgg aaatacttat tggcggtttg cttatgacca caaagctttt
     8581 caacggcgtg accggtctga gcagggtccc tctgccgtat gcaaaggcac agcttgaagc
     8641 catgggatct tttcttgttt tttcatccac tgccgggctg catcttgcct gccccaaaac
     8701 agcaaagggc acatctgctc caaggctttc tcccagaatg caaagttcct ttgtccccag
     8761 ggaaagtccc cataaaacat tgagcccgtg aagtacagcc gcagcgttcc cgctaccccc
     8821 ggcaagaccg gcggccacag ggatcctttt tttgatgaat atatcaactt cacccaaaag
     8881 tcccctgccg tgatgctttg ccataagtgc tgccgcttta aatgctatat tgtcttcacc
     8941 ggcgggtaca tattttttat cggaagaaac atttattttt attccgcatc cggctttttt
     9001 gttttggtca tttacaggac ccggtatccc ggaagatttc cagcacaatg tgacgtcgtc
     9061 atggagcatg atctgatgca ttatcatttc gaccctgtga tatccgtctt cccttttgcc
     9121 tgttatatct atggaaagat ttatttttcc atatgaattg atggttacct ctttcatggt
     9181 ttctcccgca aaatcaaaat gggcggaaaa gccgcccatt ttttatttat cagtgtttct
     9241 tctttttact ctttttcttg cttttctgac ttgctttttg tttttccttt tcttcggcga
     9301 tcctggcggc ttttgccctt ccagtcctgt aggtgcttcc tgccagtgag atcggcatgg
     9361 gttttgatac ccttatattg tctgaagata tttcaacttt gttttcttct ttatggcgtt
     9421 ttttcttggc ctcatccttt gcggcctgtt tttccttctt tttctgctcc gccgcaacta
     9481 tctcttcaac ggattttccc gttcttttgg cttctttata gggatttact gcttcttctt
     9541 ttttcctgac atccttttgg gcctgctttt ttgcttctct tgatgtgaag aatatcattc
     9601 ccaccaccat tatgcccatc atcaccatac tgacagtcat actggactga gggttcagag
     9661 tcttgaagga tacagttacc ggtttttcca ttatgttgcc cttgctggaa acaaagccag
     9721 cctcaatggt cagtttgtat tttgtgctcc cctttatctt ggctcccttt tctgtgtcgg
     9781 ccagcaccat cattatgttt ttttcttttg gattgaaaac cgttcttaca ggtacctttt
     9841 ttccttttgg atctgtaagt ttacagcgtt ttgcatttgc cttctggttg tctttggaat
     9901 aaatgtcttc attaaaatac actttcacgc ccatgttatc cacggacatt cctgtttctc
     9961 cgtttttggg agaagtttct tcaacgccga aaggtgccgc tgatgcaaaa gctgtactaa
    10021 gcataattat caatagtgaa aggcttatca gtacgcctgc tcttttcatg ttttgtcttc
    10081 ctccgatttc tttgttctca gttttttgaa gaactctttc atcatgcttt cacattcttc
    10141 ctgcatgatg ccagtttctg tttctgtata atgattcagt ctgctgtcat ttattatatt
    10201 cagcaccgat ccgcatgcgc cggctttttt gtccatggtg cctatataaa gtttttttag
    10261 tctggccaaa acgatggctc ccgaacacat gctacagggc tccatagtca cataaatatc
    10321 acagcctgtc agtctccatc ccccgagatt ttctgtagcc tgtcttatgg cgatgatttc
    10381 cgcatgggct gtggggtctt ttgagctttc tgttaagtta tgtcctctgc cgattattct
    10441 gccttctttg actattacgg ctcccaccgg cacttcccca aggtcaaagg ccctgcgggc
    10501 ttctattaaa gcttcctgca tatacctgtt catatacctt tgcatattaa catatttcgg
    10561 ctgactattc aacaaaatcg tgttagaata aacaaaacta aaatggaaaa agcaggtaac
    10621 ccaccatgac aaaagaaaaa gcaaaaaaaa ataaaataag tcttgtcggc aagatagtct
    10681 ttgtgatttc ccttgtgctc ctggtcattt cccttggcat tctcatcgac cactacatga
    10741 aaggtcaaaa aagcgaaaag gagttttctt ctcttaaagt ttccggaacc tacgaccttg
    10801 atgcccttta caggcagaac tccgacatca tcggatggat aaaaatagaa gacacaaacg
    10861 ttgattaccc ggttatgcag acaagatctc agccggagtt ttatctgcgc agaaatttca
    10921 agaaagaata ttccctttcc ggcactcctt ttatggacgc cgccagtgat ataactaaac
    10981 ctacaaaaaa ctggctcata tacggccaca atatgaaaaa cggcaccatg ttccacgata
    11041 tactgaatta tgaggatgcc ggtttttacg aaaaacataa aatttttact tttgacacca
    11101 tagaagaaaa tggcaagtgg gaagtggttg ccgttttccg aagcaaaata tacaaaaaaa
    11161 actccggtaa attcaaatac tatgaatacg gaggtatagt ttcaggagaa cagctaaaag
    11221 aatatgtcgg tatctgcaaa tccctttctc aatatgacac gggagtggaa gttaatgagg
    11281 atgacacggt cctgacgctt tcaacatgtg cctatcatac caaagacggc aggttcgcag
    11341 ttgtggcaaa gaaaacagaa taagccccgg caacttcccg gtgtcgggca aaacaggcaa
    11401 aaaaaattat gtaaaatcag aaaagaaagc cctctagaag caccattata aagttgttgt
    11461 catacagctc tccggcaaaa taagagtttt ccagagtttc cactatcatc tcagaagaat
    11521 ccggatttat cagatttgtc acaggcagca aaagtgcaag gaatataaat actatgacag
    11581 ccccttttat gaatccgact atcaggccaa aaaaaccatc agcaaatcca gcgaatccgt
    11641 cgcggtaatc tcttgagaga agtctcaaga taagccacag tattattttc aggaaagcaa
    11701 gcaacgcaat aaataccaga actgtgaaaa ccagacctgc aaatatttct gccattttgg
    11761 acgaaactat ttcagcggaa ttttggagga tatcgcttat attaagaggt acactttttt
    11821 ctgaggtttc cattacgggc acagaatcag aaaatctgag gtttagatcc tcttcaatgg
    11881 cttcatatac cgtagttttt tccacaagaa actcttttac aaaaggagtt gcaagatatg
    11941 ccagcaccgc tgccagtatc catcccacag tatgaatgaa agtaaaaacg aacccttttc
    12001 taaggccgta tactgtagtt cctatgagta tcaccgctac tatgatatca agaaccatgg
    12061 atcctccttt ccggtaaaat gattttcttc atgactacag atttaataat actaaacata
    12121 aacataaatt gcaacagttc ctgcagggca aagtctaatg attataaata tgatcctgta
    12181 agtagaacaa ttatcctttt tctaaaagtg aatgatctcc aagctttcat aatcaaaaca
    12241 tatttttgct atggtaatca catattttta ctgtatactg aaaaaaatta aaggagaaag
    12301 ggaaaggtaa aaggaaaatg aaacaaaaaa aagaaacttc tgataacaat atgttttatg
    12361 acgaagaaaa aaaactcagc tttatctcaa gacttttaag gtagcttatc cttggcagtt
    12421 ttcttttatt catcatttct tctagcatat tacacacctt ggggtatgcc gccccctcca
    12481 tacacgggtt atgtccctat ggaggactcg catcatctct gagtataata gcctatggaa
    12541 cattttttaa aaagataatg atcgggactt tcattatatt tatttcaaca atgtctctag
    12601 caattgtttt tcgaagatct ttttgcggac agatatgtgc ttttggagcc cttcaggagt
    12661 ttttagggaa tctgggccgc agtatcttta aaggcagaag atttgttatt acaactaaaa
    12721 tcgaccgtat attcaggtat cttaaatacc ttgttctgat ttttactgtg gcaatgtcct
    12781 ggatgaccgg cattctttgg atcaccccgt attgtcctta taatgcactg ggacatatgg
    12841 cagattttca atcattgaca agtatatatc ttatcgggtt tatactactt attataaccc
    12901 ttataggttc atttttttac gagcgttttt tctgtaaata tctgtgccct gcaggagctt
    12961 tttatgcaat tatcggcaaa ataagcccct tcgcagttcg catcaacaaa gataagtgta
    13021 ttgattgcgg cctttgcaac aaaatatgtc caatgaatgt aaatatcatg aattcaaaga
    13081 gcgaaaaaat tacagattct gaatgtatca actgcaatga atgtgtcaat acgtgcccga
    13141 aaaaaggtgc attaaatgca ggtttcagca aaaaaactgt tctgcatcca atattggcaa
    13201 ctcttttagt tctggcactg tttttcacac ctattattgc tgccaaatca acagattcat
    13261 ttcaaatatt acccaataaa ttcgggagtg gatctgggat ccatgaaaat ggaaaagata
    13321 aaggtctcaa tgacaaagga caaagtaaaa tcggtaatgt ttatatgaaa gatatcacgg
    13381 ggtctatgac aatgtcagaa atatcctcat tgcttgattt gtcaataaaa aacctgtata
    13441 taaaaattgg ccttcctgag aattatccgg aaaataatac tataaaacag gccgcagaaa
    13501 gtcttggatc aactgtaagc gatttgaaaa ataaaatctt tgaatagatc atttgatccc
    13561 ccatgccgtc aagaagattt tgtcaaaaca aaaaccatct ttttttaatc tcttgcgagc
    13621 agatggtttt tgtatggtga ccctggtggg attcgaaccc acgacccccg ccttcggagg
    13681 gcgttactct gtcccctgag ctacagggcc ctctatcaca gtaaacaatt ataacaaaaa
    13741 gagcggttca tgtcaccgct cttttttagt ggcacccccg agatgattcg aacatccgac
    13801 gcacggttta ggaaaccgac gctctatccc ctgagctacg ggggcacgac acgatattta
    13861 taatagcata cactcctgcc gttttcaaca aattttgctt tataccggtt tgtcatgtgc
    13921 cggttcaccg gactcaccat atcccggttt ggcagcttcg tcatatcccg ttttatgccg
    13981 gttcgacagg ttcatcaggt ttgacattta tacgctcaag tatataatga ttttgtgttg
    14041 aaagtgcatc ccctgaaatc aagaggaggg gcgcgcaggc attttgtgat gccgctttgg
    14101 catttgaaag gaacccgtaa gtatgacaac aaaacagcag ggtatcttgc ttatgtgcac
    14161 ttctgccctg ctcttttcgg caatgcagat agccataaaa tcctcagcag atgatatccc
    14221 acttatggaa caggtgtttt tcagaaacat cgtaagtctc gttataagtt ttataataat
    14281 caaaaaaaac aacggttctc tctttggcag caaagagcat cagccgttgc ttttcgtccg
    14341 ttctgtattc ggttttctgg gacttatggc aatgttttat gcggcaggac atgcaaatca
    14401 gggggatgta tccactctta tcaaactctc tccttttctt ataacccttt gggccgctat
    14461 ttttctgaaa gaaaaaatca aaaagataca agtaccggcc ctcatactgg cttttaccgg
    14521 tgcgattttt gtggcaaacc ccgcatggac atcaaatatt ttcccgcttt ttaccgcatt
    14581 gttgtgtgcg tttttttcaa gtgtgtcata tacgctcctt gcctatttca aaaacaaagt
    14641 ggacggcatg acaataataa tgcatttttc aactttctgc gttttgtgct ctctgccctt
    14701 tatgcttatg gattttgtta tgccgggaac ataccagctg ctgcagctgc tgctcatagg
    14761 atttctggga ggcttcggtc agatagccct gacttattcc tatcgcatgg ctcctgccgg
    14821 cgagataagc atatacaatt attcgggcat tattttttct attattctgg gattcctttt
    14881 cctgggagaa aggcttgcat ggaactcctg gatcggcatc ttctttgttc ttttggcatc
    14941 atttctggta tacagatttt cagggtctga cgttgatacc tctttataaa aatgttataa
    15001 tgtttttttg tgcaggaggt tgccatgaag aattataaca gtcctttttc tcaaagatac
    15061 tcaagcaacg agatgaagta tttgttttct tccgaaaaca aattccgcac ctggagacgt
    15121 ctttgggtcg ctctggccga agccgaaaag gaactgggta tcaaaataac cggggctcag
    15181 atagatgaac ttaggtccaa cataacaaac ataaattatg ctgcggccga aaaaaaagaa
    15241 aaagaagtcc gtcatgatgt gatggctcac gtttatgctt acggccttca atgtccttcg
    15301 gcaaagggca taatacacct cggtgccact tcctgttatg taacagacaa taccgatctt
    15361 ataataatga aagatgccct tttggttgta aaaaagaagc tgataaatgt tttgtccgct
    15421 ctttcaggtt ttgcaaacaa gtacaaaagt cttcccactt tgggatacac acattttcag
    15481 ccggcccagc ccacaaccgt gggaaaaagg
//
LOCUS       DAONHK010000009        15274 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_3050_length_15274_cov_4.374006, whole genome shotgun sequence.
ACCESSION   DAONHK010000009 DAONHK010000000
VERSION     DAONHK010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 15274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15274
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3050_length_15274_cov_4.374006"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="PLM92_00785"
     CDS             <1..371
                     /locus_tag="PLM92_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPR23712.1"
                     /translation="SESTGVLEKPGVIHAAVLKNCIDSRKSLDKCRKYKAKRIVSPHY
                     GIIPAYYNEEYWDLYEKAMEHEEMFIKKLHEKGLSAEEMLKEYTKHFWKEDRAKEQPI
                     EAFRVNAASIIRAYRENMHT"
     gene            378..1394
                     /locus_tag="PLM92_00790"
     CDS             378..1394
                     /locus_tag="PLM92_00790"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HPR23713.1"
                     /translation="MERNCFFDNFKVLLIFMTVFAHFIRMSGDFQRDSVGGFIYILAF
                     SFIMQGWFFISGYFSKNVEKCRNTAVKTFLIPYFLFMPLMFIMRYALFGSAKLNLMIP
                     SVALWFLLAMFVYRLAIKDLSRIRGLIPVSALLYFAVGYIPFLGKELALGRIFSFLIF
                     YVAGFYFKWEYIEKIKKTPVVLAASILSLLLLFTFLVSYYRVFPSTIWYLKECNEVFG
                     MDILVGTAVRLMIAAVSSAWLIVLVRFIPDRNYRFTSVGKYTLYIYLLHIPVRYLYEK
                     FGYLAFNDYIELAAALVGTLIILWIFSRPMTAFVYENIMNFIYKTIIKCVTIFKDILF
                     KVKK"
     gene            1391..2641
                     /locus_tag="PLM92_00795"
     CDS             1391..2641
                     /locus_tag="PLM92_00795"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HPR23714.1"
                     /translation="MIERRQLDCGINVVMEKIPHVRSVSMGIWVKAGATDETAGNSGI
                     SHFIEHMMFKGTEKRSAKKIAEDVDEIGAQINAFTGKEATCYYIKTLEENIDRSLDVL
                     TDMFLCSCFDKAEMKKEKNVIYEEIKMIRDTPDDDVHDMICEQVFKNHPLGRSVIGTP
                     SSVKGISRNMIKEYIKNEYTRDSIVISVSGSFNSDMICEKIQGIMEPLQKSKKKKTYP
                     RQLYSPFFRMKQKDIQQAHICLGTRSISLTDERYYAFSCLNNIFGGSMSSRLFQKIRE
                     EKGLAYSVYSMNSSFSRDGYFNIYAGVATDKIYDTVCAIKQEIETISTKGVSEEELKK
                     AKEQLKSAYIFGQENVNGRMISMGRNLTLVGRVYETKEVLDGIDAVTADDIKKVSKII
                     SDPKKYSAAAISNKRVALKEMVQA"
     gene            2658..3092
                     /gene="dut"
                     /locus_tag="PLM92_00800"
     CDS             2658..3092
                     /gene="dut"
                     /locus_tag="PLM92_00800"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005845350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HPR23715.1"
                     /translation="MKVKIKSISGRLPEYKTKGSAGMDICAYIEDPLMILPGQRVLVP
                     TGIFIELPRGYEAQLRARSGLAIKKGLGMVNGIGTIDSDYRGEIKIPLINMGSEKITI
                     EDGDRIAQMIIAEYQRIEWQEADELSETERSGGGFGHTGIKK"
     gene            3101..3724
                     /locus_tag="PLM92_00805"
     CDS             3101..3724
                     /locus_tag="PLM92_00805"
                     /inference="COORDINATES: protein motif:HMM:NF013982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HPR23716.1"
                     /translation="MIGIKKKEDIFFTMFVDFAGKIVTAGEAFQDLVHNYENVEEKVA
                     NMKVLETECDMETHKILKSLHESFITPFDREDIYAITKEMDSIVDCMEEVANRFQVFE
                     VKAIKPEAVVLADMTLQSIRELETMFRHLAELKKNSIVMEQIIEVNRIENEGDVVYRK
                     ALSTLFREEKDPIELIKWKHLYEQIEESIDSCENVANILEGVVMKYA"
     gene            3811..4803
                     /locus_tag="PLM92_00810"
     CDS             3811..4803
                     /locus_tag="PLM92_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003148258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HPR23717.1"
                     /translation="MFSTVLIFVIIFAYIFEFINGFHDTANAIATSVYTKALTPKRAI
                     ILAACMNFVGALVSEKVAMTISQGIVNICLEMYVIMAALIGAILWNLFTWWRGIPSSS
                     SHALIGGLIGASIVYTAGTENIIWSGVLEKVVIPLFTSPIVGFLIGFLFMKSIFKIFI
                     EWSQRKVNKVFLKLQIFSSAFVAFSHGNNDAQKTMGIITLALITGGILDESTGVPLWV
                     KFTCAITMALGTSLGGWKIMKTMGGSVTKLEPASGFAAQTTSAIVIESMTFFGAPVST
                     TQVITTSIMGAGSAKRATSVKWGIAQNIAVAWILTLPVAMILGGCACWLISYMV"
     gene            4808..5752
                     /locus_tag="PLM92_00815"
     CDS             4808..5752
                     /locus_tag="PLM92_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HPR23718.1"
                     /translation="MIGREEIEKREYEILSTYASKAAETKGRAYSEEKCNIRTEYQRD
                     RDRIIHSKAFRRLMHKTQVFLAPEGDHYRTRLTHTIEVSQIARTICRGIGLNEDLAEA
                     IALGHDLGHTPFGHIGEKALNDIHEGGFRHNIQSLRVVDVLEGDQKKGGMNLTAEVRD
                     GILNHTGPGVPFTLEGQVVKTSDRIAYINHDIDDAIRSGIIKQEELPKDCIKHLGKDH
                     RTRINTMVSDMIKSSYDKNIISMSRDVLKYMNELRDFMFGSVYLNSSVKKNEDNEEVE
                     LIIKTLYDHYGKNENAKDMIAGMTDRYAIKTYEKLKKN"
     gene            5812..7581
                     /gene="dnaG"
                     /locus_tag="PLM92_00820"
     CDS             5812..7581
                     /gene="dnaG"
                     /locus_tag="PLM92_00820"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HPR23719.1"
                     /translation="MNGAIRENTVDEIKSRCNIVDIIGGVVQLKKAGSNYKGLCPFHK
                     EKTPSFVVSEEKQIYTCFGCGATGDVIEFVQKYYNLDFPEALQKLADSCGVNIESSYT
                     GAKNKDDLYSINKEAATFFFRAFHKKGDPAYEYMKKRGINDDVLKKFGIGYADSEWDS
                     LYKHLKEKGFPEEKLIDLGLISSSKGKYFDKFRNRVIFPIINTRGKVIGFGGRTIGED
                     MPKYLNSRESSVFLKKNNLYGLSVTRREMTKTGYCVLVEGYMDVISLYQSGVCNVAAS
                     LGTALTENQAKLLKRYVSNVVLAYDSDEAGRKAALRGMDILHREGFKVKIPDIPDAKD
                     PDDFIKAKGRSAFIKLMKEALPYADYKITAIKKEVNMKTTEGRVDFLKKAVEVLKTLG
                     PVETDIYIKKLAVETGISEGAIRAEMDIGKEGKKEIRLRSGGYEEKQKKDKEPMSRLE
                     KNAIKLMLAEETYISDFADYSDIFESIAGKNIFEAISSLSRKGERAGSEKLSGMLEPE
                     DTDVLKDIQETIILADKEEEVFKECIRAYHENANKKREKELIMMISMANEEENSEEIK
                     KLTEELMQIQKKNKNGGKRSDIR"
     gene            7553..8710
                     /gene="rpoD"
                     /locus_tag="PLM92_00825"
     CDS             7553..8710
                     /gene="rpoD"
                     /locus_tag="PLM92_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007941338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HPR23720.1"
                     /translation="MGEKEVIYGDEVNEKKKEYVSELARKAKGKKLSYAEIGHYLENT
                     ELDKDQMEDIYEALMSKGIEVVSEEAPEEFELKDIESEIEEEVVPDSEKEEGEKEISL
                     EKTIPKTVAVDDPVRMYLKEIGKVPLLSADEEIDLAKRMEAGEEAAKQKLCEANLRLV
                     VSIAKRYVGRGMLFLDLIQEGNLGLIKAVDKFDWRKGYKFSTYATWWIRQAITRSIAD
                     QARTIRIPVHMVETINKLMRVSRQLLQEYGREPMPEEIAKEMDISEDKVREILKIAQE
                     PVSLETPIGEEEDSHLGDFIPDEDVPAPADAAAFSMLKEQLVEVLDTLTEREQKVLKL
                     RFGLEDGRARTLEEVGKRFDVTRERIRQIEAKALRKLRHPSRSKKLKDYLE"
     gene            8753..9511
                     /locus_tag="PLM92_00830"
     CDS             8753..9511
                     /locus_tag="PLM92_00830"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPR23721.1"
                     /translation="MQKLNDRLKAIASNISPGETVADIGTDHGFLPLFLWEKEISPKT
                     ILTDVSEKSLQKAKANAGAPQFGNQLDFRTGYGLSVIDPGEADVIVIAGMGGIVIKKI
                     LEKDPDKTKTFKKLLLQPRNNAGKLRCWLCRNGFYISGEYLAREGKYICQIIKAIPKE
                     ERGMPDAAGSIYDKQLSKEFNDIAPDDIRWEIPPWIFEKKNELASEFLKRALSTEEKI
                     LENIKKSKKVDEKEEEKIKTGINYLRSISLEEER"
     gene            9512..10318
                     /locus_tag="PLM92_00835"
     CDS             9512..10318
                     /locus_tag="PLM92_00835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HPR23722.1"
                     /translation="MKYVKLIKTIEEIAPLELAPLWDNSGVQIYTGQEEIERVLICLD
                     ITEEVIKEAENKKTGMIITHHPLIFDSIKKIDTDQVTGRYVCRLIKAGICVYSAHLTF
                     DNAREGNNFYMAKLLGMENLKTAAKERPGEGSKDPAELPSGMTGQLCRQMTPEEAASH
                     IINALEIPQDGIRMVKGKNTIKKIGLCTGAGGDFLDYTIREGCELFITGDVKLHEAQR
                     AKAEGISLIDAGHYGTEHIFAENFARQLKEKAGKELEIIESTVDTDPFML"
     gene            10360..10605
                     /locus_tag="PLM92_00840"
     CDS             10360..10605
                     /locus_tag="PLM92_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPR23723.1"
                     /translation="MDPHENEKGFVISFLIKLLLSVLVVVAAMAFAFGSGATGSEKII
                     TVVMTIVVCVIFSFFVFIEVRKFIYAWQEAQARKKIE"
     gene            10615..10957
                     /gene="rnpB"
                     /locus_tag="PLM92_00845"
     ncRNA           10615..10957
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLM92_00845"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            11107..11286
                     /locus_tag="PLM92_00850"
     CDS             11107..11286
                     /locus_tag="PLM92_00850"
                     /inference="COORDINATES: protein motif:HMM:NF023665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3606 domain-containing protein"
                     /protein_id="HPR23724.1"
                     /translation="MEKDKKKKDYSGCTTINAKDPGEINYWCGKLCCTQQQLTEALKD
                     EGNSVDKIKKRFKKD"
     gene            11523..12155
                     /locus_tag="PLM92_00855"
     CDS             11523..12155
                     /locus_tag="PLM92_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPR23725.1"
                     /translation="MKKLLTVMLVLVMAFAFASCGGGSEGDGEGDDVSKSKLTGVFGE
                     IGKESGIHMNYEMEIDGKTTVTDIYMKDDKIYTEMDMEGLGKSITIYTDGYMYTLTPE
                     TKTGIKTKVDEDTQETLETDTVLENYEEYTGDVDFEKGEKEVDGKTYYSETYKSGDSS
                     VIYCFDGDKLVYMISVTGEGEETIIKYNAIDGDVDDSIFEVPDGYTITEM"
     gene            12339..13736
                     /locus_tag="PLM92_00860"
     CDS             12339..13736
                     /locus_tag="PLM92_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006875800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HPR23726.1"
                     /translation="MDRRSEKENKMGVMPVGKLIVTMSWPAILSMMIQATYNVVDSIF
                     VARVSEKALTAVTLIFPAQMLIIAVGVGTAIGVNSLIARRLGARRFVEADKAASHGIR
                     LAVYSWIFFAVAAALFADVFMKGFTHDPYILQNGALYLRIVMIFSLFIFVQMTAEKIL
                     QATGSMILPMICSLMGACVNIALDPILIFGLLGAPEMGVTGAAVATVISQTLSFSLGM
                     YILFRKNHQVKVRIYGFPHDKKTIREIYAVGFPAIMMQSILSFLQFGINIILAGFSGT
                     AVAVMGVYGRLQSFIFMPVIGINQGTTPVIGYNYGAGNKTRLMQTYKMALLAAVVIMA
                     AGCTLFMIMPRTLLSFFDAGEGMYAIGIPAMRIISICFLPAAFGIITSGFFQATGHGF
                     ISLAASLIRQLVGILPLAIVLSRLWGVTGVWWAFPMAEALGVLFLVIMMRRLYKKEIS
                     RLVPIGQKENDRKTD"
     gene            13769..>15274
                     /locus_tag="PLM92_00865"
     CDS             13769..>15274
                     /locus_tag="PLM92_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006000614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HPR23727.1"
                     /translation="MKLGIDVGSTTVKLVLLDDHNKIIYSRYERHMSNVFEKVSELMT
                     DLEKEYGEESVKASITGSGGLSLSKLLDICFEQEVISCSKAVESLIPRTDVAIELGGE
                     DAKITFYGQTIEQRMNGTCAGGTGAFIDQMAVLLNTDANGLNEAAKKHRLIYPIAARC
                     GVFAKTDIQPLINEGADIEDISASIFQAVVNQTISGLACGRTIKGNVAFLGGPLSFLS
                     ELRKRFIETLDLGADQVIFPDESKYFVAIGAAMLAEKNESVKIKQLVDTVKNMDPDMM
                     SETKHIKPLFENHGDYEFFRQRHDADRIKRRDIKKAKGKLFLGIDAGSTTTKAALIDD
                     EKNLLFSFYRSNEGKPIEATMAMLRQLYMQLPEVAFIANTTVTGYGEGLIKAAFKADL
                     GEIETMAHYKGAEEFLPGVDFILDIGGQDMKCMKIKDGAIYNIMLNEACSSGCGSFIE
                     TYAKSVNMDSKAFAQEALFARSPVDLGTRCTVFMNSKVKQAQKEGATIGDIS"
BASE COUNT         5242 a         2752 c         3624 g         3656 t
ORIGIN      
        1 ccagtgaaag cacgggtgtc ctggagaaac ccggtgttat acatgcggct gtattgaaaa
       61 actgcataga ttcgagaaaa tcacttgata aatgcaggaa atacaaggca aaacgcatag
      121 tatctcccca ttatggaata atacctgcct actataacga agaatactgg gacctttatg
      181 aaaaagccat ggaacatgaa gaaatgttca taaaaaagct ccacgaaaaa ggtctttctg
      241 ctgaagagat gcttaaggaa tatacaaaac atttctggaa agaggatcgg gcaaaagaac
      301 agcccattga ggcatttcgc gtaaatgcag caagcataat aagggcatac agagaaaata
      361 tgcatactta aactgttatg gaaagaaact gtttttttga taatttcaaa gttcttttga
      421 tatttatgac agtttttgcc cacttcataa gaatgagcgg agactttcaa agagattcgg
      481 taggcggatt catttatatt cttgcctttt cctttataat gcagggatgg ttcttcatct
      541 ccggatattt ttcgaaaaat gtggagaaat gcaggaatac tgccgtaaag acatttctca
      601 tcccctactt tctgttcatg cctctcatgt ttatcatgag atacgcactt ttcggcagtg
      661 ccaaattgaa tcttatgatc ccctcggtgg cattatggtt ccttctcgcc atgttcgtat
      721 acaggttggc catcaaagac ctttcaagaa tcaggggact catacctgtt tcggcactcc
      781 tttattttgc ggtcggatat attccttttc tgggaaagga acttgctctt ggcaggatat
      841 tttcctttct tatattttat gtggctggct tttactttaa atgggaatat atagagaaaa
      901 tcaaaaagac ccctgttgtg ttggctgcat caatactgtc actgctttta ctgtttacat
      961 ttctggtctc gtattacaga gttttccctt cgaccatatg gtatctgaaa gaatgcaatg
     1021 aagtatttgg aatggacatt ttagttggaa cggcagtaag acttatgata gcggcagttt
     1081 cctctgcatg gctcattgta cttgtcagat ttatacctga caggaattac agatttactt
     1141 ctgtggggaa gtatactctg tatatttatc ttcttcatat cccggttaga tacctgtatg
     1201 aaaagtttgg ttatctggca tttaatgact atattgagtt ggctgcagca ctggttggta
     1261 cccttattat tttgtggata ttttcccgtc cgatgacggc ctttgtatac gaaaatataa
     1321 tgaatttcat atataaaacc ataattaaat gtgttacaat atttaaggat attttattta
     1381 aggtgaagaa atgatcgaaa gaagacaact tgattgcggc ataaacgtgg tcatggaaaa
     1441 aataccacac gtaagatccg tttcaatggg catatgggtc aaagccggag caacagatga
     1501 aaccgccggg aattcaggta tctctcattt tatcgaacac atgatgttca agggaacaga
     1561 aaaaagaagc gccaaaaaaa tcgcagagga tgtggatgag ataggtgccc agataaatgc
     1621 ttttacaggc aaagaagcca cctgctatta cataaagacc ctcgaagaaa atattgacag
     1681 atcccttgat gtcctcactg acatgtttct ttgctcatgc tttgacaaag ccgagatgaa
     1741 aaaagaaaaa aatgttatat acgaagaaat aaaaatgata cgggatacgc cggatgatga
     1801 tgttcatgac atgatatgtg aacaggtctt caaaaatcat cccctgggaa gatctgttat
     1861 agggactcca tcgtcagtaa agggcatttc ccgcaatatg atcaaagaat atataaaaaa
     1921 tgaatataca agagacagca tagtaatatc ggtttccgga agcttcaaca gtgacatgat
     1981 atgcgaaaag atccagggga taatggaacc tctgcaaaag tcaaaaaaga aaaaaacata
     2041 tccaaggcag ctttacagtc cctttttccg gatgaaacaa aaggatatcc agcaggcgca
     2101 tatatgtctt gggacaagaa gcatcagcct gaccgatgaa agatactatg ctttttcatg
     2161 cctgaacaat atcttcggcg gcagcatgag ttcccgtctt ttccagaaaa taagagaaga
     2221 aaagggactt gcctattcgg tatattccat gaacagttcc ttttcccggg acggttattt
     2281 caatatatat gcgggagtgg caacagataa gatatatgat actgtctgtg ccataaaaca
     2341 ggaaatagag accataagca caaaaggggt atcagaagag gaactgaaaa aagcaaaaga
     2401 acagctgaaa agcgcatata ttttcggtca ggaaaatgta aacggaagaa tgatatccat
     2461 gggcagaaac ctgactctgg taggcagagt atacgaaaca aaggaagttc ttgatggcat
     2521 cgatgcagtt actgccgatg acataaaaaa agtatcaaaa ataataagtg atcctaaaaa
     2581 atacagtgca gcggcaatat ccaataaaag ggtagctctc aaggaaatgg tacaggcata
     2641 aaatcatgga ggatcaattg aaagtaaaaa taaaaagcat atccggaaga cttccggaat
     2701 ataaaacaaa aggttctgcc ggaatggata tttgtgccta tattgaagac cccctgatga
     2761 tacttcccgg ccaaagggtg ctggtgccca caggcatatt catagagttg cccagagggt
     2821 atgaagcaca gctcagggcc cgtagcggac tggccataaa aaagggcctt ggaatggtaa
     2881 acgggatagg taccatcgac agcgattaca gaggagaaat aaaaatacct cttatcaata
     2941 tgggaagcga aaaaataacc atagaagacg gagatcgtat tgctcagatg ataattgcag
     3001 agtaccagcg tatagaatgg caggaagcag acgaattaag tgaaacagaa aggtccggag
     3061 gcggattcgg gcatacagga attaagaagt gaggtaaaaa atgataggaa taaagaaaaa
     3121 ggaagacata ttttttacaa tgttcgtgga ctttgcaggc aaaatagtaa cagccggcga
     3181 agcttttcag gacctggttc acaactatga gaatgttgag gagaaagtgg caaatatgaa
     3241 agtcctggaa accgaatgtg atatggagac ccataaaata ctaaaaagcc tgcatgaatc
     3301 atttattact ccctttgaca gagaggacat ctatgccata accaaagaga tggacagcat
     3361 agttgactgt atggaagaag tcgccaacag atttcaggtt tttgaggtaa aggccataaa
     3421 gccggaagca gtggtactgg ctgatatgac tctgcagagc ataagagaac tggaaaccat
     3481 gttcagacat ctggccgaac tgaaaaagaa ttcaatagta atggaacaga tcatcgaagt
     3541 caaccggata gaaaacgaag gggatgtggt ttacagaaaa gccctttcaa ctcttttccg
     3601 tgaagaaaaa gatccaattg agctcataaa atggaaacat ctttatgaac agatagaaga
     3661 aagcatcgat tcttgtgaaa atgttgctaa tatacttgaa ggagtagtaa tgaaatatgc
     3721 ctaaaagaca tgcaagacca ggtagacttt ttttgctagt atcaatatgg tttgtcggac
     3781 tgatcatatg agccggagaa gggaaaataa atgttttcga cagttttgat ttttgtaata
     3841 atttttgctt acatttttga attcataaac ggattccatg ataccgcaaa tgctatcgcc
     3901 acatccgtat atacaaaagc ccttacacca aaacgtgcca ttattctggc ggcatgtatg
     3961 aactttgtag gagcactggt aagtgaaaag gtggcaatga ccatatctca gggtatcgtg
     4021 aacatatgcc ttgaaatgta tgtaataatg gcagctctaa taggagccat actttggaac
     4081 cttttcacct ggtggagagg gatacccagc agctcatccc atgcgcttat cggaggcctt
     4141 atcggagcat cgatagtata tacagcagga acagagaata taatatggag cggagtactt
     4201 gagaaagtgg tgatacccct tttcacttca cctattgtgg gatttcttat cggctttctg
     4261 tttatgaaga gcatattcaa aatatttatt gagtggtcac agagaaaagt gaataaagtt
     4321 ttccttaagc tccagatatt ttcttcggct tttgtagcct tttcccacgg caacaacgat
     4381 gctcagaaaa ctatgggaat aataactctg gcactgatta caggaggcat actggatgaa
     4441 tcaacaggag ttcctctatg ggtcaaattc acatgcgcca taacgatggc actgggcaca
     4501 tcacttggcg gttggaaaat aatgaaaacc atgggaggca gcgtaacaaa actggaacct
     4561 gccagcggat tcgcggcgca gaccacctcg gccatagtaa tcgaaagcat gacttttttc
     4621 ggagcgccgg taagtacgac tcaggtcata acaacgtcca taatgggagc cggcagtgcc
     4681 aagagggcaa cttcagtcaa gtgggggata gcccagaaca tagccgtggc atggatactg
     4741 acgctgccgg tggcaatgat actgggagga tgtgcatgct ggctcataag ctatatggta
     4801 taaaagaatg ataggaagag aagaaataga aaaaagagaa tatgaaatac tctccaccta
     4861 tgcctcaaaa gcggcagaaa cgaaaggccg tgcctactcc gaagaaaaat gcaacataag
     4921 gacagagtat caaagggaca gggacaggat aatacattcc aaagctttca gaagactaat
     4981 gcacaaaaca caggtgtttc tggcaccgga aggcgatcat tacaggacaa ggcttactca
     5041 caccattgaa gtctcacaga tagcaaggac aatatgcaga ggtataggac tcaatgagga
     5101 tctggccgaa gccattgctc tgggtcatga cctgggacac actcccttcg gacacatagg
     5161 ggaaaaagcc ctgaatgata tccacgaagg aggatttcgt cataatatac agagtctcag
     5221 agtagttgat gtactggaag gggatcaaaa aaaaggggga atgaatctta ccgcagaggt
     5281 cagagacggg atattgaatc ataccggacc aggtgtccct ttcacattgg aggggcaggt
     5341 agtaaaaacc agcgacagga tagcgtatat aaaccatgat atagacgatg ccataagaag
     5401 cggcataatc aaacaggaag aactcccgaa agactgcata aaacatctgg gaaaagatca
     5461 cagaacaagg ataaacacca tggtaagcga tatgataaaa agcagttatg ataaaaacat
     5521 catatccatg agcagggatg ttctgaagta tatgaatgaa ctgagagact ttatgtttgg
     5581 cagcgtatac cttaacagca gtgtaaaaaa gaacgaagac aatgaagaag tggaactgat
     5641 cataaaaact ctttacgatc attacggaaa gaacgaaaat gccaaagata tgattgccgg
     5701 catgactgat agatatgcca taaaaacata tgaaaaactg aaaaaaaatt aaagtaatat
     5761 aaccgttgcc gtcgtaagtg taaatataga tgatttaccc gggagaaaaa ggtgaacggt
     5821 gcaataagag aaaatacagt tgatgagata aaaagcaggt gtaatattgt ggacattata
     5881 ggcggagttg ttcagctcaa aaaagcaggc agcaactaca aaggcctatg ccccttccac
     5941 aaggaaaaga caccttcttt tgttgtttcg gaagaaaaac agatatacac ctgttttgga
     6001 tgcggggcaa cgggggatgt tattgaattc gttcagaagt attataatct ggattttccg
     6061 gaagctcttc agaaactggc ggattcctgc ggggtcaaca tcgaaagcag ttatacgggc
     6121 gcaaaaaaca aagatgactt atattccata aacaaagaag cagcaacttt ctttttcagg
     6181 gcttttcata aaaaaggaga tcccgcatat gaatacatga agaaaagagg cataaatgat
     6241 gatgtcctca agaaattcgg catcggatat gcggattcgg agtgggacag tttatataaa
     6301 catctcaaag aaaaaggctt ccccgaagaa aaacttattg atctgggatt gatttccagc
     6361 tcaaagggta aatacttcga taagttcaga aaccgtgtga tttttcccat aatcaacaca
     6421 agggggaagg taataggttt tggaggaaga acaataggtg aggatatgcc caagtatctt
     6481 aactccagag aatcttcggt ctttctgaaa aaaaacaatc tctacgggct ttctgtaaca
     6541 cgacgggaaa tgacaaaaac aggatattgt gttttggtgg aaggatatat ggatgttata
     6601 tctctttatc agagcggtgt atgtaatgtg gccgcatctt tgggaacggc ccttaccgaa
     6661 aatcaggcaa aacttctgaa acgttatgta tctaacgtgg tgcttgcgta tgattccgat
     6721 gaagcgggac gcaaggcagc cttacgtggt atggacatat tgcacagaga ggggttcaaa
     6781 gtcaaaatac ccgatatacc cgacgcaaaa gatccggatg atttcataaa agcaaaagga
     6841 agatcagctt ttataaaact gatgaaggaa gctcttcctt atgcggatta caagatcact
     6901 gcaataaaaa aagaagtcaa catgaaaaca acggaagggc gtgtggattt tcttaaaaaa
     6961 gcagttgaag tgctcaaaac acttggccct gtggaaacgg acatatacat aaaaaaactt
     7021 gctgtagaaa cagggatatc cgaaggagcc ataagagccg agatggatat tggcaaggaa
     7081 ggcaaaaaag aaataaggct ccggtcgggc ggatacgaag aaaaacaaaa aaaagacaaa
     7141 gagcccatga gccgtcttga gaaaaatgcc ataaagctga tgctggcaga agagacatat
     7201 ataagcgatt ttgctgatta cagtgatata tttgaaagta tagcgggtaa aaatatattt
     7261 gaggccataa gcagtctgtc aagaaaaggt gaaagagcag gttccgaaaa actttcggga
     7321 atgctggaac cggaagatac ggatgtactc aaagacatac aggaaacaat aatacttgcc
     7381 gataaagaag aagaagtctt taaggaatgt ataagggcat accatgaaaa tgccaacaaa
     7441 aaaagagaaa aagaacttat tatgatgatc tcaatggcca acgaagaaga aaacagtgaa
     7501 gaaatcaaaa agctcacaga ggagcttatg caaatacaga agaaaaacaa aaatggggga
     7561 aaaagaagtg atatacggtg atgaagttaa cgagaaaaag aaagaatatg taagtgaact
     7621 ggccagaaag gcaaagggca agaaactgtc ttacgcggag ataggtcatt atctggagaa
     7681 taccgagctt gataaggatc agatggaaga catatatgaa gcacttatga gcaagggcat
     7741 cgaggtggtt tcggaagagg cgcctgaaga attcgagctg aaagatatag aatccgaaat
     7801 agaagaagaa gtagttcccg attctgagaa ggaagaaggc gaaaaagaaa taagcctcga
     7861 aaagaccata ccgaaaacag tggcggtgga tgacccggta aggatgtatc ttaaagagat
     7921 aggaaaggtt ccgcttttga gtgcggatga agagatagat ctggcaaaaa gaatggaagc
     7981 aggggaagag gcggcaaaac agaaactctg tgaggcaaac ctgagacttg ttgtcagtat
     8041 agccaaaaga tatgttggca gggggatgct gttcctcgat cttatccagg aaggaaatct
     8101 ggggctcata aaagccgtag acaaattcga ctggcgaaaa ggttacaaat tcagcactta
     8161 tgccacatgg tggatcagac aggccataac aagatcgata gccgatcagg caaggaccat
     8221 aaggatcccg gtccacatgg tggagactat aaacaaacta atgcgggtat caagacagct
     8281 tcttcaggaa tacggaagag aacccatgcc cgaagagata gcaaaagaga tggacatttc
     8341 cgaggataag gtaagagaaa tactgaagat agcccaggaa ccggtttccc tggaaacacc
     8401 cataggtgaa gaagaggaca gtcatctcgg agatttcata cctgacgagg atgtcccggc
     8461 tcccgcggat gcagcggcat tttctatgct gaaggaacag ctggttgaag ttcttgacac
     8521 tcttaccgaa agggaacaga aagtgttgaa actgcgtttc ggactggagg acggaagagc
     8581 caggaccctt gaagaagtgg gaaagagatt tgatgtcacc agagagagga tcagacagat
     8641 agaagcaaag gccctgagaa aattaagaca tcccagtcgc agcaaaaaac tgaaggacta
     8701 tctcgagtag gcatgtttgc cctgctcaaa atatcctttt cggagaaaga tcatgcagaa
     8761 gctgaatgac agacttaagg ctatcgcctc caatatatct ccgggagaaa ccgtcgcgga
     8821 tataggaaca gatcatggtt tcttgccgct ttttctgtgg gaaaaagaaa tatcacccaa
     8881 aaccatactt acagatgtca gcgaaaaatc actgcagaaa gcaaaggcaa atgccggtgc
     8941 cccgcagttc ggaaaccagc tggactttcg taccggctat ggtctctcgg taatagatcc
     9001 gggagaagcg gatgttattg ttattgccgg aatgggtggt atcgtaataa aaaaaatcct
     9061 cgaaaaagat cctgacaaaa caaaaacctt caaaaaactg ttgttgcagc ccaggaacaa
     9121 tgccggtaaa ctgcgctgct ggctttgccg caacggtttt tatatatccg gcgaatattt
     9181 ggccagggaa gggaaatata tatgtcagat aataaaagcg ataccgaaag aagaaagggg
     9241 catgccggat gctgccggta gcatttatga taagcagtta tccaaggaat tcaatgatat
     9301 tgcacctgat gacataagat gggaaatccc cccctggata tttgagaaaa aaaacgaact
     9361 ggcatcagaa tttctcaaaa gagccttatc aacagaagag aagatactgg aaaatataaa
     9421 aaaaagcaaa aaagtcgatg aaaaggaaga agaaaaaata aagacaggga taaactactt
     9481 gagaagcatt tctcttgagg aggaaagata agtgaaatat gtgaaactta taaaaactat
     9541 agaagagatc gctcctttgg agctggcccc tttgtgggat aacagcgggg tccagatata
     9601 cacggggcag gaagagatcg agagggtact tatttgtctt gatataaccg aagaagtgat
     9661 caaagaagca gaaaataaaa aaaccggtat gatcatcact caccatcccc ttatttttga
     9721 cagcataaaa aaaatagata cagatcaggt cacaggaaga tatgtctgcc ggctgataaa
     9781 agccgggata tgtgtatatt cggctcattt gacctttgac aatgccaggg aaggcaacaa
     9841 tttctacatg gcaaaactgc tgggcatgga aaacctgaaa actgcagcaa aagaacgccc
     9901 gggagaagga tcaaaagacc ctgcagagct accttcggga atgacggggc agctttgcag
     9961 gcaaatgact cccgaagagg cagcaagtca cataataaat gcccttgaga tcccccaaga
    10021 cggtataaga atggtaaaag gtaagaacac tatcaaaaaa atcggtctgt gtacaggagc
    10081 agggggagat tttcttgatt ataccataag ggaaggatgt gagcttttca taacaggaga
    10141 tgttaaactc catgaagcac agagggcaaa agcagagggc atatccctga tcgatgccgg
    10201 ccattacggt acagagcata tatttgcaga gaactttgca cggcagctga aagaaaaagc
    10261 cggaaaagaa cttgagataa ttgaaagtac tgttgataca gaccctttta tgctataata
    10321 taatgtcaat gatatcaaca tacaaaggaa ggtaaaaaaa tggacccaca tgaaaatgaa
    10381 aaaggatttg tgatttcttt tttgattaaa cttttgttgt cggtgctggt ggtggtggcc
    10441 gccatggcat ttgcattcgg aagcggtgct accggaagtg agaaaatcat aacggtggta
    10501 atgaccattg tagtatgtgt aattttttct tttttcgttt ttattgaagt aagaaaattc
    10561 atttatgcct ggcaggaagc acaggcaagg aaaaaaatag aatagacaag gttttgggca
    10621 agccagacgg tcgcatgctt tgcatgagga aagtccgggc tccaaagggc aagggtgccg
    10681 gataacgtcc ggcgcaggta actgtaggga aagtgcaaca gaaacacacc gccgaaacgg
    10741 gtgatgccgt ggcaagggtg aaatggtgag gcaagagctc accggcgttc tggagacagg
    10801 acggccgtgt aaaccccacc cggagcaaga ccaagcaggg attatggctg acggcccgtc
    10861 agctcccggg aggtaggtcg catgagcata gcagcaatgt tatgcctaga tagatgatcg
    10921 tcaaatacag aacccggctt acagacttgc ccaaaactat tataaaaaaa ctaaaaatga
    10981 acctttgagc tgacaacaca cataactaaa atgttgattt attttgaatt ttctgttatc
    11041 atataggaac atcgataaat gaggctagta cataatcaaa tgcttgagat ttttggaggt
    11101 gattttatgg aaaaggataa aaagaagaag gattacagcg gttgtacaac gatcaacgca
    11161 aaggaccccg gagaaatcaa ttactggtgc ggtaaattat gctgtacaca acagcagctg
    11221 acagaggcac tcaaggacga aggcaattct gttgataaaa taaaaaaacg tttcaaaaaa
    11281 gactgaagct ttgatttttg aaggttttca caaaaaacaa aaaccttata aataaaagaa
    11341 aagcttgccc ccgcaaaaga caaaaaactt ttttcggggg cattgtttat tccgatttgc
    11401 agcaaaatat attgaaaaaa acaaagataa agtctatact aaaaaaaggc acattgagag
    11461 aagatattcc tcaaatgatt gttgaacggg atatattttt attaattatt gggaggtaaa
    11521 aaatgaaaaa actgctaact gttatgctgg tgcttgttat ggcatttgct tttgcatcat
    11581 gcggcggagg ctcggagggc gatggtgaag gcgacgatgt ttccaagtca aagctcacag
    11641 gtgtttttgg agagataggt aaggaatccg gtatccatat gaattatgag atggaaattg
    11701 acggtaaaac tactgtaacc gatatttata tgaaggacga caagatctat acagaaatgg
    11761 acatggaagg tctgggaaaa tcaataacca tatacactga cggctatatg tacactctta
    11821 cacctgaaac caagaccgga ataaaaacaa aggtcgatga agatacacaa gagacccttg
    11881 agaccgacac tgttcttgag aactatgaag aatatacggg cgatgtggat tttgaaaagg
    11941 gcgagaagga agtggacgga aaaacatact attccgaaac atacaaatca ggtgatagca
    12001 gtgtgatcta ctgtttcgac ggcgataaac tggtttatat gatttcagta accggagaag
    12061 gcgaagagac aataataaaa tacaatgcca ttgacggaga tgtggatgac agcattttcg
    12121 aagtgccgga cggatacaca ataactgaaa tgtaaataag attttttgcg gacaaaaagt
    12181 tttatcctat aaacgccttt ggttgaatca gagcgataaa agtagtataa ttcaagttct
    12241 gcaaaggcgt tttttagttg ttaaaaacaa aaaaacaaaa acaaaaaaac acaagaaaaa
    12301 attatataag cataaaaagc gaaaaaacgg aggaatgaat ggaccgcagg agcgaaaaag
    12361 aaaacaaaat gggagttatg ccggttggaa aacttatagt taccatgtca tggccggcta
    12421 tcctgtcaat gatgatacag gcaacatata atgtagtgga cagtattttt gttgccaggg
    12481 taagcgaaaa agcactgact gcagttacac tcatcttccc ggcacagatg cttataatag
    12541 ccgtaggagt gggaacagca ataggtgtga attctcttat tgccaggaga ctgggtgcaa
    12601 gaaggtttgt tgaggccgat aaggccgctt cccacgggat aaggcttgca gtatacagct
    12661 ggattttttt tgctgtggcg gcggctttat ttgcggatgt cttcatgaag ggatttaccc
    12721 atgacccata catccttcaa aacggggctt tgtatttgcg tatcgttatg atcttctcct
    12781 tatttatttt tgtacagatg acagcagaaa aaatactcca ggccacagga agcatgattc
    12841 ttcccatgat atgcagcctt atgggagcct gcgtaaatat agccctggat cctatcctta
    12901 ttttcggact cctgggggcc cctgaaatgg gagtgacagg cgcagctgtt gcaactgtaa
    12961 tatctcagac attgagtttt tccctgggaa tgtatattct ttttcgaaaa aatcaccagg
    13021 taaaagtcag gatttacgga ttccctcatg acaaaaaaac tatacgggaa atatatgccg
    13081 ttggatttcc ggcaataatg atgcagtcta tactgtcatt tcttcaattc ggcatcaaca
    13141 tcatactggc cggattttcg ggtacagctg tggctgtgat gggagtatac ggaagactgc
    13201 agtcctttat atttatgcct gtaataggaa taaatcaggg aacgacaccg gtaataggat
    13261 acaactacgg tgctggcaac aaaacgagac ttatgcagac atacaaaatg gcacttctgg
    13321 cggcagttgt cataatggca gccggttgta cactttttat gataatgccc agaacactgc
    13381 tttcattttt tgatgcgggg gaaggcatgt atgccatagg aatacctgcc atgcgcatta
    13441 tcagtatttg ctttttaccc gctgcatttg gtataattac atcaggattc tttcaggcca
    13501 caggccatgg attcataagt cttgctgcct cgctgataag acagctggtg ggcatacttc
    13561 ctctggctat tgtactttcc aggctgtggg gagttaccgg cgtatggtgg gcttttccaa
    13621 tggcagaagc cctgggagtc ctctttcttg taatcatgat gagaagactg tacaaaaaag
    13681 aaatcagcag actggtgccc atagggcaga aagagaatga ccgaaaaacc gactgaggag
    13741 atgtcggttt ttaagacaag gggaaaagat gaaacttggt atcgatgttg ggtccacaac
    13801 agtaaaactg gtccttttgg atgatcataa caaaataata tacagcagat acgaaaggca
    13861 tatgtccaac gtttttgaaa aagtatccga acttatgacc gatcttgaaa aagagtacgg
    13921 agaagaaagt gtaaaagcct ccatcacagg gtcggggggt ctttctctgt ctaaacttct
    13981 tgacatatgt tttgagcagg aagttatctc atgcagcaaa gcagtggaat ctctgatacc
    14041 ccgtactgat gtggccattg aactgggcgg agaagatgca aaaataacct tttacggcca
    14101 gactatagaa cagcgtatga acggtacctg tgccggtggc accggagcat tcatagacca
    14161 gatggctgtc cttcttaata cggatgccaa tggccttaat gaggcagcaa aaaaacacag
    14221 acttatatat cccatagctg ccaggtgcgg tgtatttgcc aaaacagata tacaacccct
    14281 cataaatgaa ggagcagaca tagaagacat atccgcttcg atctttcagg ccgtagtgaa
    14341 ccagactatc agcgggctgg cctgcggcag gactatcaaa ggaaatgtgg cgtttctggg
    14401 aggcccgctt tcatttcttt cggaactccg taaaagattt atcgaaactc tggatcttgg
    14461 tgcagaccag gtcatctttc ctgatgaatc aaaatatttt gtggccatag gtgctgccat
    14521 gctggcagaa aaaaatgaaa gcgtaaagat aaaacaactg gttgatacag taaaaaatat
    14581 ggatcctgat atgatgtccg aaacaaaaca tataaagccg ctttttgaaa atcatggaga
    14641 ctatgagttc ttcagacaga gacatgatgc tgacaggata aagagaaggg acataaaaaa
    14701 agcaaagggg aagctgttcc ttggcatcga tgccggatcc accaccacaa aagctgctct
    14761 tatagatgat gaaaaaaacc tgttgttctc tttttacaga agcaatgaag gaaaaccaat
    14821 agaagcaacc atggccatgc tgagacagct gtatatgcag ttgcccgaag ttgctttcat
    14881 agcaaataca acagttaccg gatacgggga aggccttata aaagccgcat tcaaggcgga
    14941 tctgggagag atagagacca tggcccatta taagggtgcc gaagaatttc ttccgggagt
    15001 agatttcatt cttgatatag gcggccagga catgaaatgt atgaaaataa aggatggggc
    15061 catctataac ataatgctca atgaagcctg ctcatccggt tgcggatcat ttatcgagac
    15121 ctatgcaaaa tccgtaaaca tggacagcaa ggcatttgct caggaagcgc ttttcgccag
    15181 atcaccggtg gatctgggga cacgatgtac tgttttcatg aattccaaag tcaaacaggc
    15241 tcaaaaagaa ggagcgacca tcggagatat ttcg
//
LOCUS       DAONHK010000010        15196 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_3067_length_15196_cov_4.245294, whole genome shotgun sequence.
ACCESSION   DAONHK010000010 DAONHK010000000
VERSION     DAONHK010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 15196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15196
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3067_length_15196_cov_4.245294"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..94)
                     /gene="mraW"
                     /locus_tag="PLM92_00870"
     CDS             complement(<1..94)
                     /gene="mraW"
                     /locus_tag="PLM92_00870"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005337792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytosine(1402)-N(4))-methyltransferase"
                     /protein_id="HPR23728.1"
                     /translation="MKFSHMPVLLNESIDYMNIKKDGIYADGTLG"
     gene            complement(84..488)
                     /gene="mraZ"
                     /locus_tag="PLM92_00875"
     CDS             complement(84..488)
                     /gene="mraZ"
                     /locus_tag="PLM92_00875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HPR23729.1"
                     /translation="MFLGEYENSIDAKNRIIIPSKFRKELGVTCYLSKGPDNCLTIYP
                     EAQWQEHIEKLRSLPTNKAGARNNLRKYTSNSGEAEVDGQGRITIPQKFFRLIGFGRD
                     VVSVGCIDKIEIWDRAEWEKVSGVPDDEEYEV"
     gene            760..1515
                     /gene="lgt"
                     /locus_tag="PLM92_00880"
     CDS             760..1515
                     /gene="lgt"
                     /locus_tag="PLM92_00880"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006001937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HPR23730.1"
                     /translation="MVSYNTAPDPVAFELFGIDIRWYGILMALGILVGGLVFYTRAPR
                     HGISRESVLDILIVAIPFGIIFSRLYYVIFTWENYSGDLWQILNFRGGGLSIHGSLIG
                     GILGAYIVARVKKIDFPEGLDAVVPGFAIAQAIARWGNFFNSEAHGGPTDLPFGIMVM
                     GEKVHPTFLYESIWCLLLFFFLLWVDNRRKFKGQTALLYGMLYSAERFFVEALRTDSL
                     MIGPFRQAQVLSLLLIIVCGILYIFFSKKQKKS"
     gene            1538..2635
                     /gene="ychF"
                     /locus_tag="PLM92_00885"
     CDS             1538..2635
                     /gene="ychF"
                     /locus_tag="PLM92_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009530971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HPR23731.1"
                     /translation="MKLGITGLPNVGKSTLFNAITKAGAEAANYPFCTIDPNIGVVTV
                     PDERVDRLSGIYHSKKTIYTTIEFCDIAGLVKGASKGEGLGNQFLGHIRNVEAIVHVV
                     RCFSDSNVVHVDGRIDPLSDIETINTELILSDMEILDRRILRTSKMLKSDKTVKKELD
                     ILQELYEWLGQGKTARTMDMEDEEAEFVKTLDLLSFKPVIYAANVSEDEAASEGETND
                     DVKKLRSFAREEGSDVIVICAKIEAELSELDDEEKKMFLEEMGISEPGLDKLIKASYR
                     LLDLISFLTTGEDETRAWTIKKGTKAPRAAGRIHSDFEKGFIRAETIAYDKLMECEGS
                     LSLAKEKGLLRSEGKEYIVKDGDVMHFLFNV"
     gene            2697..3374
                     /gene="deoC"
                     /locus_tag="PLM92_00890"
     CDS             2697..3374
                     /gene="deoC"
                     /locus_tag="PLM92_00890"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HPR23732.1"
                     /translation="MKPFSKYFDHTLLKPDASEAEIKRLCSEAVKYDFCAVCVNPCHV
                     SLASEELEKTSIRIAAVTGFPLGASEMSAKAYEAEKACGSGAVEIDMVMNIGALKEGR
                     YKYVSDDIGQVASAVYDNDALLKVIIETGMLTNEQIIKAVTIAADSGADFIKTSTGFC
                     ESGASVASVKLIRTTLDDIPGKHVQIKASGGIRDLDTAQNMIRAGAERLGTSSTVSIF
                     SEYLKRA"
     gene            complement(3385..4362)
                     /gene="pta"
                     /locus_tag="PLM92_00895"
     CDS             complement(3385..4362)
                     /gene="pta"
                     /locus_tag="PLM92_00895"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016146721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HPR23733.1"
                     /translation="MFENLIEVLKQNPRKIVFTEGNDPRILSAADRLLKDGFLEPVLV
                     GRADEVTAAAKEGGFDIEGATVYDPATYAEMDKMVEKMVELRKGKMTEEECRAALQKG
                     NYFGTMLVKMEKADALLGGATYSTADTVRPALQLIKTRPGNNIVSSSFIMVREHEDKE
                     NEMLCMGDCAINIDPKEDDLVEIAIESAKTAKVFGIDPKVAMLSYSTKGSGSGESVDK
                     VRNATEKIKEKAPELDVDGELQFDAAVSPVVAEKKCKGSKVAGRANTFIFPDISAGNI
                     GYKIAQRLGGFDAYGPVLQGLNAPINDLSRGCNAEEVYVMAIITAALKE"
     gene            complement(4386..4916)
                     /gene="hpt"
                     /locus_tag="PLM92_00900"
     CDS             complement(4386..4916)
                     /gene="hpt"
                     /locus_tag="PLM92_00900"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006001901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HPR23734.1"
                     /translation="MKKYDTVGEVMITEEEIRERAEEIGEQITNDYMGEGVILIGILR
                     GAVPWMADIMKHIALDVEIDFIACSSYGATTKTSGVVKINKDLDSGIEGKNVIIVEDI
                     VDSGITLNYLKGYLENRKPKSIRICALLDKPEGRRADIKADYVGFIVEDKFIIGYGLD
                     FNQKYRHLPYISFLKE"
     gene            complement(4923..5249)
                     /locus_tag="PLM92_00905"
     CDS             complement(4923..5249)
                     /locus_tag="PLM92_00905"
                     /inference="COORDINATES: protein motif:HMM:NF018508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HPR23735.1"
                     /translation="MQFIIGFRMMIKRIKAIRFMMKDKTVPRRKKALVVVGIIYLFLP
                     VDIIPPVLFPIAWVDDLILWIWILWHLRDTLDKYWMGEKNVNYSKKYKNKTIIDDAEY
                     EVKEEK"
     gene            complement(5253..5840)
                     /gene="yihA"
                     /locus_tag="PLM92_00910"
     CDS             complement(5253..5840)
                     /gene="yihA"
                     /locus_tag="PLM92_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003383776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HPR23736.1"
                     /translation="MIIKKAEIIAVTVKPEQYPPDNMKEIAFVGRSNVGKSSLLNLIT
                     NRRKLAKTSGSPGKTRTINFYEINEEFRIVDLPGYGYAKVSRSVTEDWGPMMEKYFGT
                     RKGLIKAVQLVDIRHGPTDKDIKMYEYLKHYGLDGLVVATKSDKISKTQIRKSVKMIR
                     EGLGMGETGMVIPVSALKKTGHDVLLDEIERVLGD"
     gene            complement(5837..8188)
                     /gene="lon"
                     /locus_tag="PLM92_00915"
     CDS             complement(5837..8188)
                     /gene="lon"
                     /locus_tag="PLM92_00915"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006316715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HPR23737.1"
                     /translation="MIEEKIEKREDGMLMLPMIPLRGMTIFPNMVLHFDIGREKSINA
                     LEKAMIENQYIFLAAQKDETTDLPTSKDFYHVGTIAKIKQMLKLPGDSIRVLVEGTTR
                     GRITEMLFEVPYFKCAVQKIEDEEPDPVPPKTEALMRSALANFDEYLALDQKLSPEIF
                     PTVATIDEPGRFADIVSSHLDIKVEEKQELLECMEVDERLEKLNKILTREIEILKIEQ
                     DISSKVKTQINRNQREYYLREQLRAIQEELGNDEDVEDEIEAWHKQLKELDIPEKTAE
                     KIEKEIKRFSKMQASSAEGTVARTYIETILALPWNKESKSEIDMKKAEGILEEDHYGL
                     SKVKERVLEYLAVVELSHRIKGPILCLVGPPGTGKTSIAKSVARAIDREFVRMSLGGV
                     RDEAEIRGHRRTYIGAIPGRVISSIKDAGTKNPVFLFDEVDKIGADFKGDPASALLEV
                     LDPEQNKDFTDHYLEIPFDLSKVMFITTANTTETIPGPLLDRMEVIEVMGYTEEEKVK
                     IAEKYLIPKKIEEHGLKAENIKITEGAVRDLINYYTRESGVRNLEREIANICRKTARK
                     TVSGKKKKFDVDQDSLEDYLGKKKYRYDKIKGEKEIGVTTGLAWTVVGGDTLFIETTA
                     VPGTGKLQLTGQLGDVMQESAKAGITYIRSVANKLGIKKGFYKNMDMHLHIPEGATPK
                     DGPSAGVTMCVAMISTLTKTRVRKDVAMTGEITLRGNILPVGGIREKVLAAHRAGIKK
                     VLLPYENEKDIEEIPEKVKKQMKFILIKTVDDAIKQVMIPKKK"
     gene            complement(8215..9480)
                     /gene="clpX"
                     /locus_tag="PLM92_00920"
     CDS             complement(8215..9480)
                     /gene="clpX"
                     /locus_tag="PLM92_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015044671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HPR23738.1"
                     /translation="MTKYDENNEIKCSFCGRPQSQVKRLVAGPDVYICNECVELCQDI
                     IAEGLQKDTGEDAGEEKFLLPTPKEIAEQLSQYVIGQEDAKKSLAVAVYNHYKRVERL
                     SKKDEDDVELQKSNIVMIGPTGSGKTLLAQTLARILHVPFAIADATALTEAGYVGEDV
                     ENILLKLLQATDFDVERAQRGIIYVDEIDKISRKSDNPSITRDVSGEGVQQALLKILE
                     GTVANVPPQGGRKHPHQDFIQFDTTNVMFICGGAFDGLEKIIQKRIGEKIIGFNSDIV
                     EKEEDTSEILKKVQPEDLLKYGLIPEFIGRLPAIVTLEELSEDALIEIITKPKNALLK
                     QYGKLFEMDNVKLEIEEDAIRRIAAKALEKKTGARGLRNIMENIMTEIMYEIPSRKDV
                     EKCIITKATVDGETRPTLVLVDEKDKAAS"
     gene            complement(9517..10098)
                     /gene="clpP"
                     /locus_tag="PLM92_00925"
     CDS             complement(9517..10098)
                     /gene="clpP"
                     /locus_tag="PLM92_00925"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HPR23739.1"
                     /translation="MTLVPYVIEQDGRGERSYDIYSRLLKDRIIFIGEQIDEHLASVV
                     IAQILFLEAEDPKKDICMYINSPGGSVTAGMAIYDTMKYVKPEISTICVGMAASMGAF
                     LLAAGQKGKRMALPNAEIMIHQPLGGISGQAEDIRIHAEWIIKTRAKLNRILCENTGQ
                     NMKKVEKDTDRDNFMSSQEAKEYGIIDKVIESR"
     gene            complement(10109..11392)
                     /gene="tig"
                     /locus_tag="PLM92_00930"
     CDS             complement(10109..11392)
                     /gene="tig"
                     /locus_tag="PLM92_00930"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HPR23740.1"
                     /translation="MKATLISKDKKEAKFKMEFTAEEFEEAVIGEYKRKKDQFEIDGF
                     RKGKAPRSIIEKHYGESVFIEGAINGIFNENYPRAIEELGLDIIDRPAVEFDNFKKGE
                     DFSCNITVAVYPEIEVKDYKGIELEKIKVSVTNNDVDEELEKLRQRNARMVSVERPAK
                     NDDTVLLDYKGFVDDEQFEGGTAEKFTLKLGSGSFIPGFEDQLLGIRSDEERDVKVDF
                     PKDYHAEKLAGKAALFKCKVHEVKEEEKPELDDDFAKDVSEFDTLEEMKKDLKKNIKE
                     QKKKHAEDQMKNKVLEKVYETNDIQVPDVMVDDEISSMMQEFDQQLRSQGLDLQKYFE
                     YLKKDPNEFREEIREDAYRKVKTRMLVAAVADAEGIEAPAEDVDEEIKIMAIQYKQDP
                     DKIKEMLGEENIGFLQKDIRMRKAMDFMFESAVFK"
     gene            complement(11419..12039)
                     /locus_tag="PLM92_00935"
     CDS             complement(11419..12039)
                     /locus_tag="PLM92_00935"
                     /inference="COORDINATES: protein motif:HMM:NF014038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HPR23741.1"
                     /translation="MEIELKYAVDNISTAERILEDEYIKSIEESRTDEELKAVYFDTD
                     DYILFRNDIAFRIRKEGQKVVASLKWNGKCSGALHSREELNINLGEGDCPEMPDPGVF
                     SESETGRELMELLKDKTLGGIMKVYVSRKTVRVDTGEGIFEIALDNGKIVAGGGTCPV
                     CEMEIELYSGNQEEMLKLGEKLQQKYGLDPEKRSKYARGLALLGKI"
     gene            complement(12164..13123)
                     /gene="arcC"
                     /locus_tag="PLM92_00940"
     CDS             complement(12164..13123)
                     /gene="arcC"
                     /locus_tag="PLM92_00940"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006362294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HPR23742.1"
                     /translation="MNETKKIVIALGGNALQSGSGPATAEEQLRVVRETCEHIADLSC
                     RGYEIAIVHGNGPQVGRILLSSETAKDVTPAMPFDVCGAMSQGYIGYHLQQSLKYALN
                     MRNRNTPVVTVATQMIVDENDPAFNDPTKPIGPFYSEEEAKKISEEKGYAMKEDAGRG
                     WRRVVASPMPRKIVEIDAVKSLWDSTIVITCGGGGVPVIEKSNGVLEGVDAVIDKDFA
                     AELLAEQVEADVLMILTEVEKVAINYNKPDQKNLSAMTLEDATKYMEEGQFAKGSMLP
                     KVQAAMKFVRAFPSKRAVITSLNKALEGLQGETGTQITFNKKK"
     gene            complement(13247..14467)
                     /gene="arcA"
                     /locus_tag="PLM92_00945"
     CDS             complement(13247..14467)
                     /gene="arcA"
                     /locus_tag="PLM92_00945"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005998975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HPR23743.1"
                     /translation="MEKGIHNYSEIGKLNKVLLHRLGEEIEGLVPDNFERLLFDDIPY
                     LKVAREEHDIFAGLLRENGIEVVYYVDETAKALADDEIRKDFVKRFLEESDVYSQAAK
                     EVLEDYLISMPPRELVGTAIAGIKKTDVNVKNANSLADYIASGYPYYTDPMPNLYFTR
                     DPGACVGNGLNVHHMSTRARRREALLLQYMFKYNKDFAPEGSNLWYDYDHDFSMEGGD
                     VLVLSKDIVAVGLSQRTTANGIERFAENILNKSSFQKILVFDIPKSRAFMHLDTVFTM
                     VDYDKFTIHPEIEGPLQIYEITLNKDGKAHFESIQGELEDILRDELKLPAVELIRCGG
                     DDAMAAQREQWNDGSNTLAIAPGVVITYERNYVTNDLLEKKGIKVITMPSSELSRGRG
                     GPRCMSCPVNRDDL"
     gene            complement(14529..>15196)
                     /locus_tag="PLM92_00950"
     CDS             complement(14529..>15196)
                     /locus_tag="PLM92_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HPR23744.1"
                     /translation="KQSVVEELAKHAGVPVWNGLTDVDHPTQILADLMTIEEHVSKPL
                     NKIKVVFCGDVRNNMSYAWMYGCAKMGMSYVAYGPSELAEEIDREVVAKAEEVAGMTG
                     ASIEVSSDPKCLEGADVIYTDIWASMGEEAEIPERVKLLTPYRVTREMLVSTGNPDVI
                     FLHCLPSFHDLETAMAKEQKEAGYDIREVTDEVFRSRNSKVFDEAENRMHTIKAVIVA
                     TIA"
BASE COUNT         3473 a         3736 c         2887 g         5100 t
ORIGIN      
        1 cccccagggt cccgtctgca tatatgccgt ctttttttat gttcatatag tcgatgcttt
       61 cattgagcag caccggcata tgactaaact tcatattctt catcatcggg cacaccggaa
      121 actttttccc attccgctct gtcccagatt tctatcttgt caatacaacc aacgctgaca
      181 acatctctgc caaaacctat gagtctgaag aatttctgag gtatagttat ccttccctgt
      241 ccatccacct cagcttctcc gctgttggag gtgtattttc tgagattgtt tcttgctcct
      301 gctttgtttg tcggcaacga tctcagtttt tctatatgtt cctgccactg ggcttccgga
      361 tatatcgtaa ggcagttgtc agggcctttg ctcaaatagc aagttacccc cagctctttc
      421 ctgaacttgg aagggattat tatcctgttt tttgcatcta tggaattctc atattcaccc
      481 agaaacacga cttttatcct ccaaaattag tgacactgcc acttctacta ttatatctaa
      541 atccacaata caccactttc ctccacatta caagcatttt gctacaaaaa atgggccttt
      601 acccctcctg ctccttcagt tgtttaccat tttattccat tttatgtcct tgggtactat
      661 atattgggca taagcaaaat ttttgttaca acatttcgtt tttccgggtt tgtttccttc
      721 tgcgttgatt ttttaccggt cacagagtat aatccagata tggtttcgta taataccgcc
      781 cctgatccgg tggcttttga actttttggt atcgatataa gatggtacgg tatacttatg
      841 gctttgggca tccttgtggg aggtcttgta ttttatacga gggcgccccg acatggcata
      901 tcaagagagt ctgtccttga catacttata gtcgccatcc ctttcggcat cattttttcc
      961 agactttact atgttatttt cacctgggaa aactattccg gcgatctttg gcaaatatta
     1021 aacttcagag gcggcggact ttctattcat ggttcactta taggtggcat actgggtgca
     1081 tatatcgtgg cccgtgtcaa aaagatagat ttccctgagg gtcttgatgc ggtagttccc
     1141 ggttttgcca tagcccaggc cattgcccgt tggggcaatt tctttaattc cgaggcccat
     1201 ggtggtccta cggatctgcc ctttggtata atggtcatgg gcgaaaaagt ccatcccaca
     1261 ttcctttacg aatccatatg gtgtctgctt ttgttcttct ttcttttatg ggtggataac
     1321 cgaaggaaat tcaaaggcca gaccgcactg ctttatggca tgctttattc cgcagaacgt
     1381 ttttttgtgg aggccttaag gaccgacagt cttatgatag ggcccttcag acaggctcag
     1441 gtcttaagcc ttctgttaat aatagtatgt ggcatacttt acatattttt cagcaaaaaa
     1501 cagaaaaaat catgaccccg ctaaaggagc aaacaaaatg aaattaggta tcacaggatt
     1561 gcccaacgtg ggcaaaagca cacttttcaa tgcaataaca aaagccggcg cggaagcggc
     1621 caattatccc ttctgcacca tagaccccaa cataggtgta gtcacagttc cggatgaacg
     1681 tgttgacaga ctaagcggca tatatcattc aaaaaaaacc atatacacca ctatagaatt
     1741 ctgtgacata gccggcctcg taaaaggcgc atccaaaggc gaaggcctgg gcaatcagtt
     1801 tctcggacat atacgtaatg tggaagccat agttcatgtg gtacgctgct tttcggacag
     1861 caatgtggtt catgtggacg gcagaataga ccctttatcg gatatagaaa caataaacac
     1921 agaacttatt ctctctgata tggaaattct tgaccgccgg atcctgcgta cttcaaaaat
     1981 gcttaaaagc gacaagactg tcaaaaagga actggatatc cttcaggaac tttacgaatg
     2041 gcttggtcag ggaaaaactg ccagaaccat ggatatggaa gatgaagagg cggaattcgt
     2101 gaagactctt gatcttctct ccttcaaacc tgtcatctat gcggcaaacg tttctgagga
     2161 tgaggcggca tccgagggag aaaccaacga tgatgtaaag aagctgcgca gctttgcccg
     2221 ggaagaagga tctgatgtca ttgtcatatg cgcgaagatc gaggctgagc tttcggagct
     2281 tgatgatgaa gaaaagaaaa tgtttctcga agagatgggt atcagcgaac cgggactgga
     2341 taaactcata aaggcctctt accgcctgct tgatcttatt tcttttctca ccaccggaga
     2401 agacgagacc cgcgcctgga ccataaaaaa aggtacaaaa gccccaaggg cagcaggtag
     2461 gatccattcg gattttgaaa aaggatttat aagggccgaa accatagcat atgataaact
     2521 tatggaatgt gaggggagtc tgtctctcgc caaggaaaaa ggtcttttgc gttcagaagg
     2581 caaagaatat attgtaaaag acggtgatgt gatgcatttc cttttcaatg tataatggac
     2641 ctgtcaaaac aaaattgaga tttacccttt gctcgggatt ttccccggag gatgatatga
     2701 aaccttttag taagtatttt gaccatacac tgcttaagcc ggatgcttca gaggcagaga
     2761 taaaacgtct ttgcagtgaa gctgtaaaat atgatttctg cgccgtatgc gtaaatccct
     2821 gtcatgtgag tctggcttca gaggaactgg aaaaaaccag cataagaatc gccgcagtta
     2881 caggttttcc gctgggtgcc tctgaaatgt cagcaaaggc atatgaggcc gaaaaggcat
     2941 gcggatcggg tgccgttgaa atcgatatgg tgatgaatat aggtgccctc aaagaaggaa
     3001 gatacaagta tgtatccgat gatatcggtc aggtggcatc cgctgtatat gataatgatg
     3061 ctcttttgaa agtaataatt gaaaccggta tgcttacaaa tgagcagata atcaaagctg
     3121 taacgatagc cgctgattcc ggtgcagatt ttataaagac gtccaccggt ttttgcgaaa
     3181 gcggtgcatc cgtagcatca gtaaaactaa taagaacgac tcttgatgat atcccgggaa
     3241 agcatgtaca gatcaaagct tccgggggca tacgggatct tgacacggct cagaatatga
     3301 taagagccgg agccgaaaga ctcggcacca gctcaacagt ttcaatattt tccgaatatc
     3361 tcaaaagagc ctgatatctg ttttctattc tttcagggct gctgttatta tggccattac
     3421 atagacctct tcagcattgc atcctcttga aagatcgttt atcggtgcat tgaggccctg
     3481 aagtaccggg ccatacgcat caaatccccc cagcctctgg gctatcttat atcctatgtt
     3541 gcctgcgctt atatcgggga atatgaatgt attggctctt ccggcaactt tggaaccttt
     3601 gcatttcttt tcggccacca ccggggatac tgccgcatca aactgcagtt ctccgtccac
     3661 gtccagctca ggtgcttttt cttttatttt ttctgtagca ttgcgaacct tgtccacgct
     3721 ctctcccgat ccggaacctt ttgttgaata tgaaagcatg gctactttgg gatctattcc
     3781 gaataccttt gcggtctttg cactttctat tgctatttca accaggtcat cttctttggg
     3841 atctatattt attgcacaat ctcccatgca aagcatttca ttttctttgt cttcatgctc
     3901 ccttaccatg ataaaggatg atgatacgat gttgtttccc ggtcttgttt ttatcagctg
     3961 aagagcgggt cttactgtat ctgcggtgga atatgtggct cctcccaaaa gggcatctgc
     4021 cttctccatt ttgaccagca ttgtaccaaa gtaattgccc ttctgcaaag ctgctctgca
     4081 ctcctcttct gtcattttgc cctttcgcag ctccaccatt ttttcgacca ttttatccat
     4141 ttcggcatat gtggcaggat cgtataccgt tgctccttcg atatcaaatc cgccttcttt
     4201 tgcggcggcg gtcacttcgt cagctctgcc cacaagaaca ggctcaagaa atccgtcctt
     4261 caaaagacgg tctgccgcac ttaggatacg gggatcgttt ccttccgtaa aaacgatttt
     4321 tctggggttt tgttttaata cctcgatcag gttttcaaac attctgtttt cctcctcgtc
     4381 aaatgttatt cttttaaaaa gcttatataa ggcaaatgtc tgtatttctg attgaaatcc
     4441 aggccatacc ctattatgaa cttgtcttct actataaatc ccacatagtc cgcttttata
     4501 tcggctctcc tgccttccgg cttgtcaagg agggcacata tccttatact tttcggtttt
     4561 cgattttcaa gatagccctt aaggtaattg agcgttatgc ccgaatctac gatatcctcg
     4621 actattataa catttttgcc ttctatgccg gaatcaagat ctttgtttat ctttactaca
     4681 ccgctggtct ttgtggttgc tccgtaactg ctgcatgcta taaaatcgat ttccacatcc
     4741 agtgcgatat gcttcattat atcggccatc caaggtacag caccccttaa tatgcctatc
     4801 agtatgactc cttctcccat gtagtcattg gtgatctgtt cacctatctc ttctgccctt
     4861 tctctgattt cctcttcggt gatcattact tcaccaactg tatcatactt tttcatgaac
     4921 tgttactttt cctccttcac ctcatactca gcatcatcta ttattgtttt gttcttatat
     4981 ttctttgaat aattaacgtt tttctcgccc atccagtatt tgtccagtgt atctctgaga
     5041 tgccaaagga tccatatcca taatatcaga tcatctaccc acgctatggg gaaaagaacg
     5101 ggagggatta tatccacagg aagaaaaaga taaattatgc ccaccacaac aagagccttt
     5161 ttcctcctgg gaacagtctt atctttcatc ataaagcgta tagcttttat tctttttatc
     5221 atcatgcgaa agccaatgat aaactgcatt ttttaatctc ctagtactct ttcgatctca
     5281 tccaaaagga catcgtgccc ggtttttttc agggcggata caggtatgac catgccggtt
     5341 tctcccatgc ccaggccttc tcttatcatt ttcactgatt tccttatctg agttttagat
     5401 atcttgtcac tttttgtggc caccaccaga ccgtccagtc cgtaatgctt aagatactca
     5461 tacattttta tgtctttgtc cgtgggcccg tgtcttatgt ccaccagctg aactgctttg
     5521 atcagacctt ttcgtgtgcc gaaatatttt tccatcatgg gtccccagtc ctctgtgacc
     5581 gatcttgaaa ccttcgcata tccgtatccg ggcaaatcaa ctatcctgaa ttcttcattt
     5641 atttcataaa aatttatagt cctggtcttg cccggactgc cgcttgtctt tgccagcttc
     5701 cgtctgttgg tgataagatt aagaagggaa gattttccca cattggaacg gcccacaaaa
     5761 gctatctctt tcatattatc cggaggatat tgttccggtt ttactgtaac ggcaattatc
     5821 tccgcctttt ttatgatcat ttcttttttg gtatcattac ctgtttaatg gcatcgtcaa
     5881 cggtttttat cagtatgaat ttcatctgtt tttttacctt ttcgggtatt tcctcaatat
     5941 ctttttcatt ctcataggga agaaggacct ttttgatccc ggccctgtgg gctgcaagaa
     6001 ctttttccct tatacctccc acaggaagga tattgcctct taaggttatt tcacccgtca
     6061 ttgccacatc ttttcttact cttgtttttg tcagtgtgga tatcatggca acacacatgg
     6121 ttacaccggc tgaaggcccg tccttgggag tggcgccttc gggtatatgc agatgcatat
     6181 ccatgttctt gtaaaaacct tttttgatgc ccagcttgtt cgccactgac cttatatagg
     6241 ttattcccgc cttggcggat tcctgcataa catcccccag ctggccggta agctgcagtt
     6301 tcccggtacc gggaacagct gttgtttcta tgaaaagtgt atcgcctcca accacggtcc
     6361 atgcaagacc ggtagtgact ccgatttcct tttcgccttt tatcttgtca tacctgtatt
     6421 ttttcttacc cagataatct tccagactgt cctgatctac atcgaacttc ttttttttgc
     6481 ccgatacggt tttccttgct gttttacggc agatattggc tatttcccgt tccaggtttc
     6541 tgactcccga ctctctggtg taataattta tcaggtctcg tacggctccc tctgttattt
     6601 ttatattttc ggctttcagt ccgtgttctt ctatcttttt cggtatcaga tatttttcgg
     6661 ctattttgac tttttcctct tctgtatatc ccatcacttc gattacttcc attctgtcga
     6721 gaaggggccc ggggatggtc tctgtggtat ttgccgtggt tatgaacatc actttcgaaa
     6781 ggtcgaaagg aatttccagg taatgatccg taaaatcttt gttctgttcc ggatccagta
     6841 cttccagaag tgccgaagcc ggatcccctt tgaaatctgc gccgatcttg tcgacttcat
     6901 caaaaaggaa taccgggttt tttgtccctg catcttttat ggaacttatt acccttccgg
     6961 gtatggctcc tatatatgtc ctcctgtgac ctcttatctc tgcttcatcc ctgactcctc
     7021 ccagagacat ccttacgaat tctctgtcaa tggcccttgc cacggatttt gcgatagatg
     7081 ttttccctgt tcccgggggg cccacaagac aaaggatggg gcctttgatc ctatgtgaaa
     7141 gttccacaac tgccaggtac tccaatactc tttcctttac tttagaaagc ccgtaatgat
     7201 cttcttccag tataccttcc gcctttttca tgtctatctc ggatttggat tctttgttcc
     7261 agggaagggc aagtatggtc tctatatatg tccttgccac tgtgccctct gcggaagaag
     7321 cctgcatttt tgaaaatctt tttatttctt tttctatttt ttctgcggtt ttttcgggga
     7381 tatcaagctc tttcagctgt ttgtgccagg cttctatctc atcttccaca tcctcgtcgt
     7441 tgcccagctc ctcctgtatg gccctgagct gttcccgtag ataatattct ctctggttgc
     7501 ggtttatctg agtcttgacc ttgctgctta tatcctgttc gattttgagt atctctattt
     7561 cccttgtgag tatcttgttg agcttttcaa gtctctcatc tacttccata cattccagaa
     7621 gttcctgttt ttcttccact tttatgtcaa gatgagaaga aactatatcg gcgaatcttc
     7681 cgggctcgtc aatggttgcc accgtgggaa atatctccgg agaaagtttc tggtccagtg
     7741 ccagatattc gtcaaaattt gccagggcag aacgcatcag cgcctcggtt ttgggaggca
     7801 cgggatccgg ttcttcatcc tcgatttttt gtactgcaca tttgaaatac ggaacttcaa
     7861 aaagcatctc tgttatcctg ccccttgttg ttccttccac caggacccgg atggaatctc
     7921 ccggcagttt gagcatctgt tttatctttg cgatcgtacc cacgtggtaa aaatcctttg
     7981 atgtgggcaa gtctgtggtt tcatccttct gtgcagcaag aaaaatatac tgattttcta
     8041 tcattgcttt ttcgagagca tttatcgact tctctctgcc tatgtcaaaa tgcagtacca
     8101 tgttggggaa aatagtcatc cctcttaagg gtatcatggg aagcatcagc atcccgtctt
     8161 ctctcttttc tattttttcc tctatcataa tatctcctta tttaataagg cgacctatga
     8221 agccgccttg tctttctcat cgaccagtac cagtgttggc ctcgtttccc catcaaccgt
     8281 cgctttggtt ataatgcatt tctcaacgtc tttccgtgat ggtatttcat acatgatctc
     8341 agtcattata ttttccatta tgttcctgag cccccgggcc cctgttttct tttccagagc
     8401 cttggcggct atccttctaa tggcatcttc ttctatttcc agtttcacat tatccatttc
     8461 gaagagttta ccgtactgct tcagcaaggc attttttggc tttgttatta tttctatcaa
     8521 ggcatcttct gacagttcct ccagagtgac tatggcaggc aacctgccta taaactccgg
     8581 tatcagtcca tatttcagaa gatcttcagg ctggactttt ttgagtattt cggaggtgtc
     8641 ttcttctttc tccactatgt ccgaattgaa acctattatt ttttcaccga tacgtttttg
     8701 tattatcttt tcaagtccgt caaaggctcc tccgcatatg aacataacgt ttgtggtgtc
     8761 gaactgtatg aagtcctgat ggggatgttt cctccccccc tggggaggta cattggccac
     8821 agttccttca agtatcttca gaagggcctg ttgcactccc tctccgctta catcccttgt
     8881 tatggaagga ttgtcactct ttctggatat cttgtctatc tcatccacat atataatgcc
     8941 tctctgggcc ctttcaacat cgaaatctgt cgcctgcagc agtttcagaa gtatgttctc
     9001 aacatcctct cccacatatc ctgcctcggt aagggccgtg gcatcggcta tggcaaaggg
     9061 tacatgaagt atccttgcca gggtctgggc aagcaaagtc ttaccggaac ctgtaggccc
     9121 tatcattacg atattgcttt tctgcagttc cacatcgtct tcgtcttttt tgctcaggcg
     9181 ctcaactctt ttgtaatgat tataaactgc aactgccaag gacttttttg catcttcctg
     9241 gcctattaca tactgggaaa gctgttctgc tatctcctta ggggtaggca gcaggaattt
     9301 ttcttcccct gcatcttctc ccgtatcctt ctgcagtccc tctgctatta tatcctggca
     9361 aagttccaca cattcgttgc atatgtaaac atcgggtcct gccaccagtc tcttgacctg
     9421 gctctggggt cttccgcaaa atgagcattt tatttcattg ttttcatcgt actttgtcat
     9481 ttttccctct gataattaaa tttttctttt attttcttat cttgattcta tcactttatc
     9541 tattattccg tattccttgg cttcctgaga actcataaaa ttgtctctgt ccgtgtcctt
     9601 ctcgactttt ttcatgttct gccccgtatt ctcacatagg atcctgttca gcttggctct
     9661 tgtttttatt atccattctg catgtatcct tatatcttcc gcctgtccgc ttatgccgcc
     9721 caaaggctga tggatcatta tttcggcatt gggaagagcc attcgttttc ccttttggcc
     9781 cgcagccaga aggaatgctc ccatgctggc cgccattccc acacatattg tggatatctc
     9841 cggttttaca tatttcatcg tatcataaat tgccattccg gcagtcaccg aaccaccggg
     9901 gctgtttatg tacatacata tatctttttt tggatcttcg gcttccagaa acagtatctg
     9961 tgctataact acactggcaa gatgttcatc tatctgttcg cctatgaata ttattctgtc
    10021 tttcaggagc cgtgaatata tatcgtatga tctttctccg cggccgtcct gttcaataac
    10081 atatggtacg agtgtcatat cctgtctcct atttgaaaac tgcactttcg aacatgaagt
    10141 ccattgcttt tctcattctt atgtccttct gaaggaatcc tatgttttct tctcccagca
    10201 tctcttttat tttgtcagga tcctgcttgt actgtatggc cattattttg atctcttcat
    10261 caacatcctc cgctggagct tctatgcctt ccgcatcggc aactgcagcc accagcatac
    10321 gggtcttgac ttttctgtag gcatcttccc tgatctcttc cctgaattca ttgggatctt
    10381 ttttcagata ctcgaagtat ttctgaaggt cgaggccctg gctcctgagc tgctgatcga
    10441 attcctgcat catgctgctt atttcgtcat ccaccataac atcaggtacc tggatatcgt
    10501 ttgtttcata taccttctcc aggactttgt ttttcatctg atcctctgca tgtttctttt
    10561 tttgctcttt gatattcttt ttaagatctt ttttcatctc ttccagagta tcgaattcac
    10621 ttacgtcttt tgcaaagtca tcatccagtt ccggtttttc ttcttctttt acttcatgga
    10681 ccttgcactt gaacaatgcg gctttgccgg ccagtttttc agcatgataa tccttgggaa
    10741 aatccacctt cacatctctt tcttcatcgg atcttatgcc caaaagctga tcttcaaatc
    10801 cgggtatgaa actgccggat cccaacttta gagtgaattt ttctgctgtt cctccttcaa
    10861 actgttcatc atccacaaat cctttataat caagaagcac cgtgtcatca ttttttgccg
    10921 gtctttcaac ggaaaccatt cttgcgtttc tctgacgcag tttctccagt tcttcatcaa
    10981 catcattatt tgtcacagat acttttatct tctcgagctc aatgcctttg taatccttga
    11041 cttcgatctc gggatatacg gcaacggtta tattgcatga gaaatcttcg ccttttttga
    11101 aattgtcgaa ttccactgca ggcctgtcga ttatatcaag ccccagttcc tcaatggccc
    11161 tcggataatt ttcgttaaaa atgccattga tggcaccttc aataaaaaca gactcgccat
    11221 aatgcttttc gattatgctt cttggtgcct taccttttct gaaaccatct atctcaaact
    11281 gatccttttt tcttttgtat tcgcctatga ctgcttcttc aaactcttcg gcagtaaact
    11341 ccatcttgaa ttttgcttct tttttgtctt ttgaaataag tgttgctttc atttttatca
    11401 gttctcctta actattggtt aaattttgcc cagcaaagca agaccccttg catatttgct
    11461 tcttttttca ggatcaagac catatttttg ctgcagtttt tctcccagct taagcatctc
    11521 ttcctgattc cccgagtaaa gttctatttc catctcacat acgggacatg taccgcctcc
    11581 ggcaacgatt ttaccgttgt ccagtgctat ttcaaaaatg ccttcacctg tatctaccct
    11641 gactgtcttt cttgaaacat acactttcat gatgccgcca agtgtcttgt cctttagcag
    11701 ctccataagt tctctgcccg tttcactctc tgaaaacaca ccgggatcgg gcatttcggg
    11761 gcaatcccct tctcccaggt tgatgttcag ttcttcccgg ctgtgcaatg cgccggaaca
    11821 ttttccgttc catttcaaag aagctacgac tttctgccct tcttttctta tcctgaatgc
    11881 aatatcgtta cggaataaaa tataatcatc cgtgtcaaag tatactgcct ttaactcttc
    11941 gtccgttctg gattcttcta tgcttttgat atactcgtct tcaaggatgc gttctgcagt
    12001 cgaaatgtta tctaccgcat acttcaattc aatttccata tggtcccttt ctttgcatgt
    12061 gaactctaat atgataccat tttttaatac aaaatacaac aaaaaaggca agatgcggct
    12121 cttttagaag tcgcatcctg tatggtcaat cattgaaatc tctttatttt tttttgttga
    12181 atgtgatttg ggttccggtt tctccctgca gcccttcaag ggctttgtta agagaagtta
    12241 tcactgccct tttgctcggg aaagctctta cgaacttcat tgccgcctgg acttttggaa
    12301 gcatgctacc tttagcgaac tgcccttctt ccatatattt cgtggcatct tcaagcgtca
    12361 tagcggaaag atttttttga tcgggtttat tatagtttat ggcaactttt tcaacctcgg
    12421 taagtatcat cagcacatcc gcttctacct gctctgccag gagttctgcg gcaaaatctt
    12481 tatctatgac cgcatccact ccttcaagta caccgttgct cttttcgatc acaggtacac
    12541 cgccgccgcc acaggttatt actattgtgg aatcccagag gctctttaca gcatcgatct
    12601 caacgatctt tctgggcatt ggggatgcca ctacgcgtct ccatcccctg ccggcatcct
    12661 ctttcatggc atatcctttt tcttccgaga tcttctttgc ttcttcttct gagtaaaaag
    12721 gccctatggg cttcgtggga tcattgaaag ccggatcatt ctcatcgact atcatctgtg
    12781 ttgccacagt aacaacaggt gtattcctgt ttctcatatt aagagcatat ttaaggctct
    12841 gctgcaaatg ataccctata tatccctggg acatggctcc gcacacatca aaaggcatgg
    12901 ccggagtaac atcctttgcc gtttccgagg aaagcagtat tcttcccacc tgaggaccgt
    12961 tgccgtgtac tatggctatc tcatatcctc tgcagctcag atcggcaata tgttcgcacg
    13021 tttcacgaac tactctgagt tgctcctctg cagtcgccgg accgctgccg gactgaagag
    13081 cattgcctcc cagagcgata acaatttttt ttgtttcatt catgatcgct ctttcctttc
    13141 ttattatttt gatcgtatct gctgatattt ccgaaagatc ggttagaaat ctttttctca
    13201 catcatttct gcagatggtc tctcaaattc attttgcaga tgctctctaa agatcgtccc
    13261 tgttcaccgg gcaactcata catctggggc cgcctctgcc tcttgaaagt tccgaactgg
    13321 gcatcgttat tacttttatg ccctttttct caagaagatc attggtaacg taatttcttt
    13381 cgtatgtaat aacaacgccg ggagctatgg ccagtgtgtt tgacccgtcg ttccactgct
    13441 ctctctgagc agccatcgca tcgtctcccc cgcatcttat gagttccact gccggaagtt
    13501 taagttcatc tctcaaaatg tcctcaagct cgccctgtat gctttcaaaa tgagctttgc
    13561 cgtctttgtt cagggttatc tcataaatct gaaggggccc ttctatctcc ggatgtatgg
    13621 tgaatttatc ataatccacc attgtaaata cagtatcaag atgcataaaa gctctgctct
    13681 tgggtatatc gaacacaagt atcttctgga agctggactt gttcagtatg ttttctgcaa
    13741 aacgctctat accgtttgcc gtcgttctct gagaaagccc cactgccact atatcctttg
    13801 aaaggaccag tacatcgccg ccttccatgg aaaaatcatg atcatagtca taccaaagat
    13861 tgcttccctc cggagcaaag tctttgttgt atttgaacat gtactgcaga agcagtgcct
    13921 ctcttcttct ggctcttgta ctcatatggt gtacattaag accgtttccc acacatgccc
    13981 cgggatccct ggtgaaataa aggttgggca tgggatctgt atagtaggga tatccggatg
    14041 cgatataatc cgccaaagaa ttggcatttt tcacattgac atcggttttt ttgatccctg
    14101 ctatggccgt tcccacaagt tctctgggtg gcatgcttat cagatagtcc tcaagaactt
    14161 ctttggcggc ctgggaatat acatcgcttt cttcaagaaa tctctttacg aaatcttttc
    14221 tgatctcatc atcagccagt gctttggctg tttcatcaac ataatatact acttctatcc
    14281 cgttttctct caatagtccg gcaaatatat catgttcctc gcgggccacc ttcaaataag
    14341 ggatatcatc aaaaagcagt ctttcaaaat tgtccggcac aagaccttct atttcttctc
    14401 ccagtctgtg cagcaggact ttgttgagtt ttcctatttc cgaataatta tgtattcctt
    14461 tttccataac ggacctcctt tgtccagagc aataccgttt ttctgtatga agtttttgta
    14521 tggatttctt atgctattgt ggcaacgata acagccttga tggtgtgcat cctgttttca
    14581 gcttcatcga aaacctttga attcctgctt ctgaagactt catcggtcac ttcccttata
    14641 tcgtatcctg cttctttctg ttcttttgcc attgcagttt caagatcatg gaaagaaggc
    14701 aggcagtgaa ggaaaataac atccggattt cctgtcgata ccagcatctc ccgggtgact
    14761 ctgtagggtg tcagtaactt gactctttcc ggtatctcag cttcttcgcc catggaagcc
    14821 catatgtcag tatatatcac atctgctccc tcaagacatt tgggatcgct ggacacttct
    14881 attgatgcgc cggtcattcc tgcaacttct tctgctttgg cgaccacttc cctgtctatt
    14941 tcctctgcca gctcagaagg cccgtatgcc acataactca tgcccatttt tgcacatccg
    15001 tacatccacg cataactcat attgtttctg acatctccgc agaaaacaac tttgattttg
    15061 ttaaggggct ttgatacatg ctcttcaatg gtcatcagat ccgccagtat ctgggtggga
    15121 tggtcaacat ctgtaagtcc gttccatacg ggaactcccg catgctttgc cagttcttcc
    15181 acaacagact gcttat
//
LOCUS       DAONHK010000011        14658 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_3232_length_14658_cov_4.796754, whole genome shotgun sequence.
ACCESSION   DAONHK010000011 DAONHK010000000
VERSION     DAONHK010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 14658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14658
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3232_length_14658_cov_4.796754"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..691
                     /locus_tag="PLM92_00955"
     CDS             <1..691
                     /locus_tag="PLM92_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPR23745.1"
                     /translation="LAPPILVKCDKKHGVPYIAVLTVAITNIILCQFAFTTVVVVDVF
                     LLVSAYVMIFISALILRKRIPREEVPFRIPGGYGFLILICIVPCIIAFIGFFINGTDY
                     FIGGMLGIVSGPVLYYIWKRMYGGLAKKDPVKYPINPKTKLAVGDLKRMAFNFIILAA
                     FGGLGVPWLGWFEGGWGPDYYIETYGSGFFADFDGMVNVIKIAAIVFAIVAVILFLLA
                     MALEPKKQKNA"
     gene            887..2296
                     /locus_tag="PLM92_00960"
     CDS             887..2296
                     /locus_tag="PLM92_00960"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPR23746.1"
                     /translation="MRAATRATRAKKHTEEQKSTLRVSLTTAFITTFMGSALNLAIPD
                     MGEYFDVSAAFLGWIVTAYMLAVATMSVPFGKVADTTGRRRVMILGILFFTLFNIISV
                     FAPNMATVIVLRTAQGVAGAMIFATNTAILISAFPGKERGKVLGKMTAATYVGLSAGP
                     FIGGFLNHYLGWQSIFGTAALVGIFAFITAVRKLPRGEKLITEGRHDYAGSILYVAMI
                     ILTMYGLSNITSVRFAWIWVLAGLLVGIFFVSVELRAANPVIQVRIFIRNIVYTLSNI
                     AALLNYGATFAIGYLMSIYLQVVMGYDSQKAGLILVAQPLIQAFFSPMAGRWSDRTEP
                     YKLASAGMGICALCLIFFSSVSLNTPLWAIITALCVTGFGFALFSSPNTNAVMACIKP
                     KDYSVASSILATMRSLGHTSSMSLVTVIVGAYMGTASLSSARPELLIKTMHTAFIVFT
                     GLCIAGIFMSLKRKTASEK"
     gene            complement(2307..3092)
                     /locus_tag="PLM92_00965"
     CDS             complement(2307..3092)
                     /locus_tag="PLM92_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009643565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP-associated domain-containing protein"
                     /protein_id="HPR23747.1"
                     /translation="MKFDLHCHTREGSIDSKVALKKYIKLLADQGFGGMMITDHDTYR
                     GCSFWEEIKKDIKKDPRYSNFVVLKGVEYDTKDAGHILVVMPDDVFLHVLALRGMTLK
                     RLMKIVRHYGGILGPAHPFGVRSSSAMFFKKLKYDPNIAKKFDFIETFNTCESAKSNE
                     MAGMMAHMYQKPGFGGSDAHEEKYVGMAYTEIHADITSNNDLIRAVREGAYVTCGGTE
                     RNKTKKSKHKNMRYAVWGFKFYNRGLGYLFSPYRKYKIRKLAI"
     gene            3662..4870
                     /gene="trpB"
                     /locus_tag="PLM92_00970"
     CDS             3662..4870
                     /gene="trpB"
                     /locus_tag="PLM92_00970"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HPR23748.1"
                     /translation="MDFRTYLKSYPDKNGYFGQYGGAHVPPELIKPFEEITEAYKTIC
                     HSAQFISELRRIRKQFQGRPTPVYHCNRLSKELGTTQIYLKREDLNHTGAHKLNHCMG
                     EGLLAKYMGKKKLIAETGAGQHGVALATAAAYFGLKCEIHMGEVDIVKQAPNVARMKV
                     LGAEVIPVSHGLKTLKEAVDSAFEAYLKDYKEAIYCIGSVVGPHPFPMMVRDFQMVVG
                     NEAREQFMEMTGYLPDAVVAAVGGGSNAMGIFPAFLNDPVSIYGVEPLGKGSKMGDHA
                     ASLTYGTKGVMHGFESIMLKDEKGEPAPVYSVASGLDYPSTGPEHAFLRESGRVNYDV
                     INDEEAIDAFFKLSKTEGIIPALESSHALAYAMKLARKEDNPGFVLVNLSGRGDKDMD
                     YVLEHYGDRI"
     gene            4969..6444
                     /gene="cls"
                     /locus_tag="PLM92_00975"
     CDS             4969..6444
                     /gene="cls"
                     /locus_tag="PLM92_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016838324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HPR23749.1"
                     /translation="MQGIMYIIESIPWTYITLSLYAVNIIIALTIIFLERKNPAATLA
                     WIMILFLLPGIGILFYFLLSQNIARKKIFKLTKDEERLLTSSLEKQTKAIETGTYEFT
                     SYEADLWKDLIRLNQIYGRAYYTQDNNVEVITDGKEKMDQLLWDIENAKESINIMYFI
                     LKRDKVGRKFIDTLTKKAAEGVKVRLLLDAMGSKNISFIFLHEFINSGGEYALFFPPK
                     LKFINMKLNYRNHRKLAVIDDTIGYIGGFNIAKEYLGLKKKFGYWRDTHLRITGGSVQ
                     DINARFLLDWRLASKEKKVEVSKVFFEPPMIAGETGIQIVSGGPDTEHDEIKRAFMKM
                     ITSASRTINIQTPYFVPDAPVLESIKMAALSGVDVRIMIPCKPDHVFVYWATYSYVGE
                     LLRDGARVFIYENGFLHAKTMTVDGEVATVGSTNFDSRSFKLNFESNAFIYDAATTRK
                     LEKIFEDDIANCRELTYEKYLKRSLWIKFKESISRLPSDLL"
     gene            6468..7190
                     /locus_tag="PLM92_00980"
     CDS             6468..7190
                     /locus_tag="PLM92_00980"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HPR23750.1"
                     /translation="MRSFFNPWTIGMLLIMAFFSLRSGTFDHPIDWLMSIVMMLPGIV
                     IGLSFHEYSHALVAYKLGDDTPKLQGRVTINPAAHMDPLGLIALIFVHFGWGKPVQIN
                     PGNFKKPRRDELLVSLSGVLMNLIIAVIFTFVLKFYVISTGYSSMSAGMGGTVWMIIF
                     YVIYINLILMIFNLLPIPPLDGFNIITEVFNLKRTDFYYKIYDKGFIILMILIVFDFT
                     GRIMTPCIMFFLRILSNITPFY"
     gene            7359..9317
                     /locus_tag="PLM92_00985"
     CDS             7359..9317
                     /locus_tag="PLM92_00985"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HPR23751.1"
                     /translation="MRTRDNNIKRIIISIFTALCMAAAMIPVDLSSVNAEETDSALQQ
                     DTTYQEEQNDYSLAGEDVPEAEINGIQYSTLQEALNAVNDGEKIKVLADIDQENLVIN
                     RPGIKFLVDLNGKTITGVTGDAAVIIEAGSAGFYDSTCEGTTGTSGTIEAAAGQQVIS
                     VTSQDLFLDASTRSGEITGLSVSFGARHSYAITSSVEPVSKVGGSITMPLTVKYGDSF
                     TYTATPSKGYSVKQILVDGINKGTSKTGTVNWVTAAVTVKAVFYRSGLFIMLDSGHYA
                     GYNKGYKGTGYYEGTQMWKLHLYLKDALEAYPGIVVDTTRARNDTTYSTTIQSYERGC
                     MARNYDLLLSLHSNASSSSATDYVISICSTKSSVKTVSQGLGLKLAEAVKATMGTNQG
                     AWVYTRKNSSGTDWYGINRGASEYDVPSVLIEHSFHTNQKMATWLMSDTNLKKLAARE
                     AYTVATYFGEANYPKPGKPGSFRATSYGYKGTKLTWSKSARAKGYRVYVSTTKNGKYR
                     YLKQLSGGTNTTNINTGLKTGKTYYYRVRSYDIVGGGVKIFSSYTSKLGAKPKPAKPK
                     NMVLKPGSKKVTVSWSKVKGATRYQIYRATSKTGKYSRIATIKGTKITNKKLKKGKNY
                     YYKVRAYRSQKGKKIYGSFCAKARAKAK"
     gene            9526..12117
                     /gene="clpB"
                     /locus_tag="PLM92_00990"
     CDS             9526..12117
                     /gene="clpB"
                     /locus_tag="PLM92_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HPR23752.1"
                     /translation="MNLEKYTNKAQEAIMECRNIAIQEGHQQLEGEHLHLALMLQHEG
                     LIPKLVKYMEADAGKIIGDLQGEIEKLPKVAGGGDNMYSTRRFEKIFLNAEKAARDQK
                     DEFVSVEHIYMALLDEKGTPSARIFDKYGITKEKFTKALMKVRGDQKITSQNPEEQYE
                     ALEKYGRDLVQMARDGKLDPVIGRDDEIRHTIQILSRRTKNNPVLIGEPGVGKTAVVE
                     GLAQRILAGDVPEGLKDKTIYALDMGALIAGAKFRGEFEERLKAVLNEIEKNEGRIIL
                     FIDELHNIVGAGKTEGSMDAGNILKPKLARGELHCIGATTLDEYRKYIEKDAALERRF
                     QKIMVDQPTVEDTISILRGLKERFEIHHGVRITDNALIACAVLSERYISDRFLPDKAI
                     DLMDEAASKIRTEIDSMPSELDQINRKIMQLEIEKQALGKESDEASGRRLENIEKELA
                     ELKNENDAMRAQWESEKKAISEVKETKQRIEDVKLEIEEAERNYDLEKLAQLKYGTLP
                     DLEKKLEEDKEQASEAKESRLLKEEVGEEEIAEVVSKWTGIPVSKLVESEKEKLLKLP
                     DILHRRVIGQDEGINAVSEAILRARAGLKDRNRPMGSFIFMGPTGVGKTELAKALSEA
                     LFDSEKNIIRIDMSEYMEKHSVSRLVGAPPGYVGYDEGGQLTEAVRRKPYSVVLFDEI
                     EKAHPDVFNVLLQLLDDGRLTDNQGRTVDFKNTLVIMTSNIGSIYLTENIKEDGTISN
                     EVKEQVVTELKKNFRPEFINRIDDIVVFSPLTEEEIEKIVDLSMKDIESKLAEKNITL
                     QLTDASKKLIAKEAYSPIYGARPVKRYLQKHIENELAKRIISGEIKEGETVTIDNKGD
                     KQDFTSR"
     gene            complement(12270..12935)
                     /locus_tag="PLM92_00995"
     CDS             complement(12270..12935)
                     /locus_tag="PLM92_00995"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HPR23753.1"
                     /translation="MPVKRTDLTGVKTTAAPLSTDLFFRIKEDILSGKLKENQKLTEQ
                     AVCDQYKVSRTPVREALLQLEMEGLVENIPNRGAYVLGLTRQDIEDMYTLRKIYEIQA
                     VRWAISRITEKEMDELEETFEFMEFYTMKNDMNKMLNININFHQVIYKASHNRMLRQL
                     LSSYQLYIKHHKKPQKDTEKYLTEVLEEHRQIFRAFKDGDVDAGVRAMEMHMDNSRER
                     NQY"
     gene            complement(12950..14089)
                     /locus_tag="PLM92_01000"
     CDS             complement(12950..14089)
                     /locus_tag="PLM92_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016146312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HPR23754.1"
                     /translation="MQYREKFDDIISRQLQRIEEMKKDSGFTDYRSLEKTVIGVIGGD
                     GIGPAITSHAQKVLSSLLSEEMKKGRIEFRVIEGLTIENRAAKGKAIPEDVLEEIKKC
                     NVLLKGPTTTPRKGDKWANVESANVAMRKALDLFANVRPVSIPEKGIDWTFFRENTEC
                     LYAMGSQGMNITEDLAIDFRVITSQGTERIARMAFDYAKENRKKKVTIVTKANVVKTT
                     DGKFLDICKKVSGDYPEIETDDWYIDIMTAKLIDEKRRKQFQVMILPNLYGDILTDEA
                     AELQGGVGTAGSANIGKHYAMFEAIHGSAPRMVLEGREKYADPCSVIRAGAMLLSHIG
                     YQEKSEKLYNAIEECTVKEKNIRLTGRDDGATGAEFADYILSKIS"
     gene            complement(14079..>14658)
                     /locus_tag="PLM92_01005"
     CDS             complement(14079..>14658)
                     /locus_tag="PLM92_01005"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005838454.1"
                     /note="Catalyzes the conversion of citrate to isocitrate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HPR23755.1"
                     /translation="KPFPRNERLKDNIKVTVVLSAGDNITTDDIMPSDSRLLPYRSNI
                     PHLSEYCFEKIDPDFPRRCKNAGSCAIAGGDNYGQGSSREHAALVPLFLGVKFVMAKS
                     FARIHRSNLINSGIIPLVFSDPSDHDRIKVGHDLVIENTLSQMKSPVITVKNLTTGEK
                     YKTETNFSETEKGIILAGGKINYIKQEADHAV"
BASE COUNT         4493 a         2917 c         3373 g         3875 t
ORIGIN      
        1 tctggcaccg cctatacttg ttaaatgcga taagaagcac ggagttccgt atatcgccgt
       61 tctgactgtt gccataacaa atatcattct atgccagttc gcattcacca cagtagttgt
      121 tgtggacgtg ttcctgctgg tttccgcata tgtaatgatc ttcatatcag cacttatcct
      181 gagaaaacgt ataccaagag aagaagtgcc cttcaggata cccggaggtt acggtttcct
      241 gattctgata tgcatagtgc cctgtataat cgcatttata ggattcttca taaatggtac
      301 cgactacttc ataggcggta tgctgggcat agtttcaggc cctgttctct attacatctg
      361 gaagaggatg tatggcggcc tggccaaaaa agatccggtg aaatatccga tcaatccaaa
      421 aaccaaactg gctgtgggcg acctgaaaag aatggccttc aacttcatta ttctggcagc
      481 attcggtgga cttggtgttc cctggctggg atggttcgaa ggcggctggg gcccggatta
      541 ttatatagaa acatacggtt caggattctt tgcagacttt gacggtatgg taaatgtaat
      601 aaagatagca gccatagtgt ttgccatcgt ggcagttata ctgttcctgc tggcaatggc
      661 actggaacca aaaaaacaaa aaaacgcctg ataaaaactg aaaatcaata aaaaagcaaa
      721 agctgctcca ttaaggagca gcttttgctt gtctattgaa atctttctta ttatcatttt
      781 tacgcttttc tgatgaacat tataaagcaa ttcttttgac ttggatcctt tcaaatgaac
      841 aaacaacgac ttgaaaaaac ccttggcagg gtgcataatt ggatttatga gggcagcaac
      901 aagagcaaca agagcaaaaa agcatacaga ggaacagaaa tcaactttaa gagtttcttt
      961 gacaacggca ttcataacca catttatggg aagcgcactg aatctggcta tacctgatat
     1021 gggagagtat tttgatgtaa gtgccgcatt tctgggatgg atagtcacgg catatatgct
     1081 ggctgtggcc accatgagtg tcccctttgg caaggtggcg gataccacag gaagaagaag
     1141 agtaatgata cttggcatac tcttctttac tttgttcaat ataatttctg ttttcgcacc
     1201 aaacatggca actgtaatag tattaagaac cgcccagggt gtggcgggag ccatgatatt
     1261 tgccaccaat acagccatac tgataagcgc ttttccggga aaagaaagag gcaaagtgct
     1321 ggggaaaatg accgcggcaa cctatgtggg gctttcagca ggtcctttta taggaggatt
     1381 cttgaatcat tatctgggat ggcagtccat ttttggcact gccgccttag tgggcatatt
     1441 cgcatttatt acagctgtga gaaaacttcc aagaggagaa aaacttatta ccgaaggaag
     1501 acatgattat gccgggagca tcctttatgt ggccatgatc atacttacga tgtacggtct
     1561 ttccaatatt acttctgtaa gatttgcatg gatatgggtt ttggcagggc tgctggtggg
     1621 aatatttttc gtttcagtgg aacttcgggc tgcaaacccg gtcatacagg taaggatatt
     1681 cataagaaat attgtttata cgctttcaaa tatagccgca cttttgaact atggagccac
     1741 ctttgccatc ggatatctta tgtcaatata ccttcaggtg gtaatgggat atgattccca
     1801 gaaagctgga ctcatactgg tagcacagcc tcttatacag gcttttttct cgcccatggc
     1861 aggcaggtgg tctgacagaa ctgaaccata caaactggcc agtgcaggta tgggaatatg
     1921 cgcactttgt cttatttttt tctcgtcggt ttctttgaac accccgctgt gggccataat
     1981 cacggcactt tgtgttactg gatttggatt tgctttgttt tcttctccca ataccaatgc
     2041 agtaatggca tgtatcaagc caaaggatta cagtgtggca tcttccatac tggcaaccat
     2101 gagatccctg ggccatacat ccagcatgtc ccttgtgaca gtgatagtag gagcatatat
     2161 ggggactgct tccctgtctt ctgccagacc ggaactgctg ataaagacca tgcacacggc
     2221 atttatcgta tttactggcc tttgcatagc aggcattttt atgtcgttaa aaaggaaaac
     2281 agcttctgag aaataaaaaa agccggctaa atagccagct ttctgatctt gtattttcgg
     2341 tatggggaga acaggtaacc cagccctctg ttgtagaatt tgaatcccca tacggcatat
     2401 ctcatgtttt tatgtttgct cttttttgtt ttatttcttt cggttcctcc gcaggtcaca
     2461 tatgcccctt ctcttacagc tcttatgaga tcgttgttgg aggttatatc tgcatggatc
     2521 tctgtataag ccatccccac atatttctcc tcatgggcat cactgccgcc gaagccgggt
     2581 ttttgataca tgtgagccat cataccggcc atttcattgg acttggcgga ttcgcacgta
     2641 ttgaaagttt caatgaagtc gaactttttt gcgatattgg ggtcatattt gagtttcttg
     2701 aaaaacatgg cgctggaact cctgactccg aaaggatgtg caggccccag gataccgccg
     2761 taatgacgga ctattttcat cagccttttc aaggtcatcc cgcgcaaagc aagaacatgc
     2821 agaaaaacat catcaggcat tactaccaga atatgtcccg catcctttgt atcatattca
     2881 acacctttta gtacgacaaa attggaatac cttggatctt ttttgatatc ttttttaatc
     2941 tcttcccaga aagaacaacc cctgtaggta tcatgatctg ttatcatcat accgccaaac
     3001 ccctggtcgg ctaaaagttt tatatatttt ttcagtgcta cttttgagtc aattgagcct
     3061 tctcttgtgt ggcagtgcaa atcaaatttc aagtatcaat atcctccttt tgatttctga
     3121 taatactata tacccggtga aaccatttta caacagaaaa tataagaaaa aagcaagtat
     3181 tatggagata catatgtttt atacaaaaaa tacagaaaaa gttgttgaca gaaaacaaag
     3241 gtggtgctat catgactgta aatcaatatt aaacctatga cgagggagta aagctgacaa
     3301 acgatttcaa agcgattccg ggcaggtgcg agccggaaag atgacagtct cagcagaatg
     3361 ggcctctgag ggcgaagcga aaagcataag caaatgctga ttagcggaga cgcgaagccg
     3421 gtgttacggg cttggggtgt gacggcactc tgcagagagg gcagataata tctgtcaaac
     3481 aaggtggtac cgcaggcata ttatgacctg tccttgtacg atagtacagg acaggttttt
     3541 tgtttgagac agtaagccta tgtactggaa aagataattg cagagaagga cccttctaca
     3601 actgaagtgt aaagaacgca gaaaaacgga ggaaaaaatg gaaaacacaa aagaaaaaaa
     3661 cattgatttc aggacatacc tgaaaagcta tcccgacaaa aatggctatt tcggacagta
     3721 tgggggagca catgtgccgc ctgaactgat caagcccttt gaagagatca cagaggcata
     3781 caagactata tgtcattcgg cacagttcat aagtgagcta aggagaataa gaaaacagtt
     3841 ccagggaaga ccgactcctg tttaccactg taacagactt tccaaagaac tgggcacaac
     3901 acagatatat ctgaaaagag aagatctcaa tcatacgggt gctcacaaac tcaaccactg
     3961 tatgggagaa gggctccttg ccaagtatat gggaaagaaa aaacttatcg ccgaaaccgg
     4021 cgcagggcag catggagtgg ctttggccac ggctgcggca tacttcggac taaaatgtga
     4081 aatacatatg ggagaagtgg atattgttaa gcaggctccc aatgttgcca gaatgaaagt
     4141 actgggagct gaagtgatac ccgtgagtca tgggctcaaa acactgaaag aagcggtgga
     4201 ttccgctttt gaagcgtatc tgaaagatta taaagaggcc atttattgta taggttcagt
     4261 tgtggggccg catcctttcc caatgatggt aagggatttc cagatggtag ttggaaatga
     4321 agcaagagag cagttcatgg aaatgaccgg atatctgcct gatgcggtag ttgcagctgt
     4381 gggcggcgga agcaatgcca tggggatatt tccggctttc cttaatgacc cggtcagcat
     4441 ttacggagtt gaacctttgg gaaaaggctc aaaaatggga gatcatgcgg catctcttac
     4501 ttacggcaca aaaggtgtta tgcacggatt tgaaagtata atgctgaaag acgaaaaagg
     4561 agaaccggca ccggtttact ctgttgccag cggtcttgac tatccttcta ccggaccgga
     4621 acatgcattt ctgagggaaa gcggcagggt caattatgat gtgatcaatg atgaagaagc
     4681 aatagacgct tttttcaaac tgtcaaaaac ggaaggcata atacctgccc ttgaaagttc
     4741 ccatgcgctg gcctatgcca tgaaacttgc caggaaagaa gacaatccgg gattcgttct
     4801 tgtcaatctt tcgggaaggg gagataagga tatggactat gttctggagc attacggcga
     4861 caggatttaa ggatttttac tgatgacagg acaccatttt caatgtataa ttaaaatggt
     4921 gttcttttat tacagagaag tgtctttttg tcggaaagga gggaaaagat gcaggggata
     4981 atgtatatca tcgagagcat accttggact tatattacac tttcattata cgcagtcaat
     5041 ataataatag cattaactat tatttttctt gagcgtaaaa acccggctgc cacattggca
     5101 tggatcatga tacttttcct tttgccgggc ataggcatac ttttttattt ccttctttcc
     5161 cagaatattg ccagaaaaaa gattttcaaa ctgacaaagg atgaagaaag acttcttaca
     5221 agttcgctgg agaaacagac aaaagccata gaaacgggaa catacgaatt tacctcatat
     5281 gaggctgacc tctggaagga tctgataaga ttgaaccaga tatacggccg tgcatactat
     5341 acacaggaca acaatgttga ggtaattaca gacggaaaag aaaagatgga tcaactgctt
     5401 tgggatatcg aaaatgccaa agaaagcata aacataatgt atttcatact aaaaagagac
     5461 aaagtcggca ggaaattcat tgatactctt acaaaaaagg ctgcagaagg agtgaaagta
     5521 agactactgc tggatgccat ggggagcaaa aacatcagtt ttatttttct ccatgagttc
     5581 ataaattcag gaggagagta tgctctgttc tttcccccga aattaaaatt cataaatatg
     5641 aaactcaatt acagaaatca ccgaaaactg gcggttatag atgacactat aggatatatc
     5701 gggggattca acatagccaa ggaatacctg gggctcaaaa agaagttcgg atactggaga
     5761 gatacgcatc ttcgcataac cggcggcagt gtccaggata taaatgcaag attcctgttg
     5821 gactggagac tggcttcaaa agaaaaaaaa gttgaagttt ccaaagtgtt ttttgaacct
     5881 ccaatgatcg ccggagaaac aggcatacag atagtatcgg gagggcccga cacggaacat
     5941 gatgaaatca agagggcttt catgaaaatg atcacctctg cctcaaggac cataaatatc
     6001 cagactccct atttcgttcc ggatgctcct gtactggaat ccataaaaat ggcagctctt
     6061 tcgggggtcg atgttagaat aatgataccc tgcaaaccgg accatgtttt tgtatactgg
     6121 gcgacttatt cttatgtggg agaacttctg agggacggag caagggtctt tatatatgaa
     6181 aacggatttc tgcatgcaaa aacaatgact gtggacggag aagtggcaac ggtgggatcc
     6241 acaaattttg atagccgcag tttcaagctt aactttgaat caaacgcttt tatttatgat
     6301 gcggcaacca ccagaaaact tgaaaaaata tttgaagatg acatagcaaa ctgcagggaa
     6361 cttacatatg aaaaatacct caaaaggtcg ctctggataa agttcaagga atcgatctca
     6421 aggcttcctt cggacctgct ttgatttttt aaaaaaggag aaacgatttg cgtagttttt
     6481 ttaatccatg gacaataggc atgttactga taatggcttt cttttcactg agaagcggga
     6541 cttttgatca tcccatcgac tggctgatga gcatcgtaat gatgctcccc ggaatagtta
     6601 taggactttc gtttcatgaa tactctcatg cccttgtggc atataaactg ggggacgata
     6661 cgccaaagct ccagggaaga gttaccataa atccggcggc ccacatggac cctcttggcc
     6721 ttatcgccct aatattcgtg catttcggat ggggcaaacc ggtgcagata aacccgggaa
     6781 atttcaaaaa accaagaagg gatgaacttc tggtttcgct ttccggagtt ctaatgaatc
     6841 tcataatagc cgtgatattt acttttgttt tgaaatttta tgtcatatca acgggttatt
     6901 cttctatgtc cgcagggatg ggaggaacag tatggatgat aatattctat gttatataca
     6961 tcaatcttat actgatgata ttcaatctgc tgcccatacc gcccctggac gggttcaata
     7021 taataacaga agtgttcaac cttaaaagaa cagacttcta ttataaaata tatgacaaag
     7081 gatttattat acttatgata ctgatagtct ttgattttac cggaagaata atgactccct
     7141 gtatcatgtt ttttctaagg atcctgagca acataactcc attctattga taagataagt
     7201 tacaaacaca ggtgtttttg aaacaacttt tatttgtgaa atttctgtga aatttacatt
     7261 ttttcacatt ttcatctttt cgtgatgtta taatctacag gcaagaatac taaaggagga
     7321 gaccggagcc ggtaaaaaag ccgagagggt ggattggaat gagaaccaga gataataata
     7381 taaaaagaat aattataagt atttttacgg cgctgtgcat ggcggcggcc atgatacctg
     7441 ttgatctttc atcagtgaat gccgaagaaa cagattcggc ccttcagcag gacactactt
     7501 atcaggaaga gcaaaacgac tattctttag ccggcgagga tgtgccggag gccgagataa
     7561 acggtataca gtacagcact ttacaagagg ccttgaatgc cgtcaatgat ggagagaaga
     7621 taaaggttct tgctgatatt gaccaggaaa accttgttat aaatagaccc ggcataaaat
     7681 ttctcgttga cttgaacggt aaaaccataa cgggtgtaac gggagatgcg gcagtaatca
     7741 tagaagcggg cagtgcagga ttttatgaca gtacttgcga agggactact ggtacgagcg
     7801 gcactataga ggccgccgca ggacaacaag ttatatcggt aacaagccag gatttatttt
     7861 tggatgcttc aacaagaagc ggagaaatca ctggtctgag tgttagtttc ggagcccgtc
     7921 attcatatgc gatcacgtct tcggtggagc cggtttccaa agtaggcggc agcattacaa
     7981 tgcccctgac agtgaaatac ggtgacagtt ttacatatac ggccaccccg agcaaagggt
     8041 attcggtaaa acagatactg gtagacggta taaacaaggg cacctcaaag acaggcacag
     8101 taaactgggt cacggcggcg gttacagtaa aagccgtatt ctacagaagc ggccttttca
     8161 taatgctgga ttcgggccat tatgcgggat ataacaaagg gtataaaggt accggctatt
     8221 atgaaggcac acagatgtgg aagcttcatc tttatctgaa agatgccctg gaagcatatc
     8281 cgggtatcgt tgtggacacc acaagggcca ggaacgatac cacatacagt actaccatac
     8341 agtcatacga aagaggctgc atggcaagaa attatgacct tcttttgtct ctgcactcca
     8401 atgcatcttc atcatctgcg actgactatg ttatttccat atgcagtaca aagtcttccg
     8461 tgaaaacagt atctcaggga ctcggtctta agcttgccga agcggtgaaa gctacaatgg
     8521 gcaccaatca gggagcatgg gtatatacaa gaaaaaacag cagtggcacc gattggtacg
     8581 gaataaacag gggagcctca gaatatgatg tccccagtgt gcttatagaa cattcttttc
     8641 atacaaacca aaaaatggcc acatggctta tgagtgacac aaacctaaag aaactggcag
     8701 ccagggaggc atatactgta gccacttatt tcggggaggc aaattatccg aaaccgggca
     8761 agcccggctc tttcagggca acatcctacg gatataaggg aacgaagctg acctggtcaa
     8821 aatcggccag agcaaaagga tacagagtat atgtttccac tacaaaaaac gggaaataca
     8881 gatatttaaa acagctttcc ggaggaacga atacaacaaa catcaatacc ggactaaaga
     8941 ccggtaaaac atattattac agagtcaggt cttatgatat cgtaggtgga ggcgtcaaga
     9001 tattcagcag ctacacttcc aagctgggcg ccaagcccaa gcctgcaaaa ccgaaaaata
     9061 tggttctgaa accgggatcc aaaaaagtta ctgtttcatg gagcaaggtc aagggagcca
     9121 ccagatatca gatttacagg gccacctcaa aaaccggtaa atatagcagg atagcaacga
     9181 tcaaaggcac gaaaataact aataagaaat taaaaaaagg taaaaactat tattacaaag
     9241 tgagagcata caggagtcaa aaaggcaaga aaatttacgg aagtttttgt gcgaaggcca
     9301 gagctaaggc aaaataaaga gaatacaaag agaatataaa gaaatttaac aggggcagtc
     9361 cggatgaagg gctgtctctt tctataaaaa agaaagaaaa cagaaaaatt atcagcactc
     9421 taaagtggag agtgctaaaa agtgtttact ttttgtttgt gacggggtaa aatgatatcg
     9481 taagtaaaaa agtcccatag gacagaaaat acaggaggtg ttagcatgaa cctggaaaaa
     9541 tatacaaaca aggcacagga agcaataatg gagtgccgca atatcgccat ccaggaaggc
     9601 catcagcagc ttgaaggcga acatcttcat cttgcgctta tgttacagca tgaagggctc
     9661 atacccaagc tggtaaaata tatggaagcc gatgcaggaa aaatcatcgg tgatctgcag
     9721 ggggagatag aaaaactgcc gaaagtggcc ggaggcggag ataatatgta ttccacaagg
     9781 agatttgaaa aaatcttttt gaatgccgaa aaagcggcaa gagatcagaa agatgaattc
     9841 gtgagtgtgg aacacattta tatggcattg ctggacgaaa aagggactcc ttctgccagg
     9901 atatttgaca aatacggtat aacaaaagag aaattcacaa aagctcttat gaaagtaaga
     9961 ggagatcaga aaatcacaag ccaaaatccc gaagaacagt atgaagccct tgaaaaatac
    10021 ggaagggacc tggtccagat ggcaagagac ggcaagctgg atccggtaat aggacgggat
    10081 gatgagatca gacatactat acagatattg tcaagaagga caaaaaataa tcccgtactg
    10141 ataggagaac ccggtgttgg taaaacagcc gtagttgaag gcctggctca aaggatactg
    10201 gccggtgatg tgccggaggg attaaaagac aaaaccatct atgctcttga catgggagct
    10261 cttattgcag gtgcaaaatt caggggagag tttgaagagc gtctgaaagc agttctgaat
    10321 gagatagaaa aaaacgaagg aaggatcata ctgttcatag atgagcttca caatatagtg
    10381 ggtgcaggaa aaactgaagg ttccatggat gcaggcaata ttctgaagcc gaaacttgca
    10441 aggggagagc ttcactgcat aggggcgacc acccttgatg agtataggaa gtatatagag
    10501 aaagatgccg cgttggagag acgttttcag aagataatgg ttgatcagcc tacggtggag
    10561 gacaccatat ccatactcag aggactgaaa gaaagattcg agatacatca tggtgtcaga
    10621 ataacagaca atgcactcat tgcctgtgca gttctttccg aaagatacat aagtgacaga
    10681 ttcctgcccg acaaggcaat agaccttatg gatgaagccg cttcaaaaat caggaccgag
    10741 atagacagta tgccttcgga actggatcag ataaacagaa agataatgca gctggagatt
    10801 gaaaaacagg ctttaggcaa agaaagtgat gaggcctcgg gcagaagact tgaaaatata
    10861 gaaaaagagc tggctgaact gaaaaacgaa aacgatgcca tgagagccca gtgggaaagt
    10921 gaaaaaaaag caataagcga agtaaaagaa accaagcagc gcatagagga tgtcaagctt
    10981 gaaatagaag aggcagaaag aaattatgac cttgaaaaac tggcacagtt gaaatacggc
    11041 acattgccgg accttgaaaa gaaacttgaa gaagataaag aacaggcttc ggaagcaaaa
    11101 gaatcccgcc ttctgaaaga ggaagtcggt gaagaagaga tcgccgaagt tgtttccaaa
    11161 tggacgggaa tacccgtaag caaacttgta gaaagtgaaa aagaaaagct tctcaaactg
    11221 ccggacatac ttcacaggag agtgataggc caggatgagg gtataaacgc agtatcggag
    11281 gccatattaa gggcaagagc aggattaaaa gacaggaatc gcccaatggg atcttttatt
    11341 ttcatgggac cgacaggcgt aggcaagacc gagctggcaa aggccctttc cgaagccctt
    11401 ttcgattcgg agaaaaacat cataaggata gatatgtccg aatatatgga gaaacactcg
    11461 gtttcaagac tggtaggcgc acctccggga tatgtgggat atgatgaagg aggtcagctt
    11521 actgaggcag taagacgcaa gccctacagc gttgtacttt ttgatgaaat agaaaaggct
    11581 catcccgatg tgtttaatgt gctcctgcag ctgctggatg acggaaggct tacagacaac
    11641 cagggcagga ccgtagattt taaaaacaca ctggtcataa tgacatcaaa tataggcagc
    11701 atttatctta ccgaaaacat aaaggaagac ggcactattt caaacgaagt caaggagcag
    11761 gtggtgaccg aactgaaaaa gaatttcaga cctgaattta taaacaggat agatgatatc
    11821 gtagttttca gcccccttac cgaagaagaa atagaaaaga ttgttgacct ttcaatgaaa
    11881 gatatagaaa gcaaactggc agaaaaaaac ataacactgc agctgacaga tgccagcaag
    11941 aaacttattg ccaaggaggc ctattctccc atatacgggg caagacctgt caaaagatat
    12001 ctgcaaaagc atatagaaaa cgagctggca aagcggataa taagcggaga gatcaaggaa
    12061 ggcgagaccg ttaccataga caacaaaggc gacaaacagg actttacaag ccggtagtca
    12121 gagacttttc ataagaaaaa gaaccttaat aagcaaccga tggtaataac agcccatatc
    12181 aaacgataat aaaaaaagag atcttaggat ctctttttaa atacttggat tttcggctta
    12241 tgtataaata ctgtttaaat ttccgtatat taatactgat tgcgttctct ggaattatcc
    12301 atatgcatct ccatggccct tactcccgca tccacatcgc cgtctttgaa agctctgaaa
    12361 atctgacgat gttcttccag aacttcggtc aggtatttct cggtatcttt ctgaggtttc
    12421 ttgtgatgtt tgatatacaa ttgatacgaa gaaagaagct gcctcagcat acggttatgg
    12481 gatgccttgt aaatgacctg atggaagttg atattgatat tcagcatttt attcatatcg
    12541 tttttcatgg tgtaaaactc catgaactca aaagtctcct ccagttcatc catttctttt
    12601 tcggtgatcc tgctgatggc ccatctgacg gcctgtatct catatatttt tctgagagta
    12661 tacatgtcct ctatatcctg ccttgtaagg cccaggacgt aggctcctcg gtttgggatg
    12721 ttttccacca gaccctccat ttcaagctgc agcagagcct ctctgacagg tgtacggctg
    12781 accttgtact ggtcacagac agcctgttcg gtgagcttct ggttttcttt cagcttgcct
    12841 gaaagtatat cttccttgat cctgaaaaaa agatctgtgg aaaggggagc ggcagtggtt
    12901 tttactcccg ttagatctgt acgtttgacc ggcatttttt ctccttcttt cagcttatct
    12961 ttgacagtat gtaatcggcg aattctgccc ctgtggcacc gtcatcacgc cctgtcaaac
    13021 ggatattttt ttctttgact gtgcattcct cgatggcatt ataaagtttc tctgattttt
    13081 cctgatagcc tatatgggaa agcagcatag ctcctgctct tattacactg cagggatctg
    13141 catacttttc acgaccttcc agaaccatcc ttggtgcgct gccgtggatg gcctcaaaca
    13201 tggcataatg ctttcctatg ttcgcacttc ctgcagtgcc tacaccaccc tgcagttctg
    13261 ccgcctcatc ggtcaggatg tctccgtaaa ggttgggtag tatcatgacc tggaattgct
    13321 ttcttctttt ttcatctata agtttggctg tcattatgtc tatgtaccag tcatcggttt
    13381 cgatttcggg ataatcaccg gagacttttt tacaaatatc caggaatttt ccgtcagtgg
    13441 ttttcacgac attggccttt gttacaatgg tgactttttt ctttctgttt tcttttgcat
    13501 aatcgaaggc catacgggct atcctttcgg tgccctggga ggttattacc ctgaagtcaa
    13561 tggccaggtc ttctgttatg ttcatgcctt gagatcccat tgcatataga cattctgtgt
    13621 tttcgcggaa aaaagtccag tctatgcctt tctccgggat acttacggga cgcacatttg
    13681 caaaaagatc cagagccttt ctcatggcca catttgcact ttccacattc gcccatttat
    13741 ctcctttgcg gggagtggtg gtgggacctt tgagaagcac attgcatttc tttatttctt
    13801 ccaggacatc ctccgggatg gccttgccct tggcggcacg gttttctatg gtaagtcctt
    13861 cgatgacccg gaactctatt cggccttttt tcatttcttc cgaaaggagt gaagagagca
    13921 ctttttgtgc atgggatgtt atggcgggtc cgataccgtc gcctcctatt acacctatta
    13981 ctgttttttc aagagaacgg taatccgtaa aaccgctgtc ttttttcatt tcttcgatcc
    14041 tttgaagctg acgggatatg atgtcgtcga atttttctct atactgcatg atcggcctcc
    14101 tgcttgatat aattgatttt tcctccggcc agaataatgc ctttttctgt ttcggaaaaa
    14161 ttggtttcag ttttatactt ttcacctgtt gtaagattct ttactgttat cacagggctc
    14221 ttcatttgag acagagtgtt ttctatcaca aggtcatggc cgacttttat acggtcatga
    14281 tccgatggat ccgaaaatac cagaggtatg ataccgctat ttataagatt gctcctgtgt
    14341 atcctggcga aagattttgc catcacgaat tttactccca ggaaaagagg aaccagtgct
    14401 gcatgctctc tgctactgcc ctgaccatag ttatctcctc cggctattgc acagctgcct
    14461 gcatttttac agcgccgggg aaaatccgga tcgattttct caaaacagta ttccgaaaga
    14521 tgaggtatgt tggatctgta cggcaacagc ctggaatcgg agggcatgat atcatccgta
    14581 gttatattgt caccggcact taagacaacg gtgaccttta tattgtcttt cagcctttca
    14641 tttctcggga aaggtttt
//
LOCUS       DAONHK010000012        14560 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_3261_length_14560_cov_5.265633, whole genome shotgun sequence.
ACCESSION   DAONHK010000012 DAONHK010000000
VERSION     DAONHK010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 14560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14560
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3261_length_14560_cov_5.265633"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(109..1383)
                     /locus_tag="PLM92_01010"
     CDS             complement(109..1383)
                     /locus_tag="PLM92_01010"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPR23756.1"
                     /translation="MRKKAKTTALMTALIMIVGIFAGCTTYDNFKEAFLGDSSGRDTV
                     KIGVFEPSTGEFKEEGELETRGMELANKLYPEVLGKKVELIYADTGSDLNVAKTAAKD
                     LVANEPSVILGSYGSVFSMLGGSYFEKKNIPAIAVTNTNPLVTSEKDNYFRVCFVESD
                     QGTALADFTAESLKNEKAAVMMPKDDDQAMEVSREYSDRMIKLTEDEDAVLSYQEYSP
                     GDVDFGRQLYRIKETGARVVFLPGAANDSLNIIRQARDLSYPFIFLGTELWQDEDFIK
                     EIKDRKEKEVFWSAVFDADARQTEMTGVFLEAYRKEYGKSAPKEATALGFDAYLLALN
                     AIEKAGDPQNGRKISEALRNNKNFEGASGFITFDQKGDPERSVNINGLSEGKVTAVYV
                     AKPASVIKAEKEKEKKEKEKEKRDKDKEKEKD"
     gene            complement(1453..2463)
                     /gene="dusB"
                     /locus_tag="PLM92_01015"
     CDS             complement(1453..2463)
                     /gene="dusB"
                     /locus_tag="PLM92_01015"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005340585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HPR23757.1"
                     /translation="MPSIGNIELENPFFFAPMAGISDAPTRSLARSMGAALVYSEMVS
                     GKGLLYNSKKTERLLNIYPEEKPLAFQIFGAEPLVIEETARILSKRDNVILDINMGCP
                     VSKVIKNNEGCALMKDPSLAGKIVEAAVKGTKKGLSEEAHSAKGNPVPGEKPVTVKIR
                     AGWDSEHINAVEVALAAQEAGAMAVAVHGRTRQQYYGGKADREIITRVKDALVIPVIG
                     SGDIFSGEDANEMMEETGCDFVMIARGALGNPWIFKEASALWQGKSVPPGPSTEEKKA
                     MIIKHLEMLEKEKGERTAVREMRKHVGWYLKGERGVAAIRCKVNEAVTVTELKQIVEN
                     LV"
     gene            complement(2467..3312)
                     /locus_tag="PLM92_01020"
     CDS             complement(2467..3312)
                     /locus_tag="PLM92_01020"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009644257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HPR23758.1"
                     /translation="MLLAFDVGNTNIVLGVFKDGELIQNWRLESDNNKSADEYGMIIN
                     QLFSYENLDIKDVEDVIVSTVVPSVLFTIQHLAVKYFRKRAIVIEPGVKTGLIIKYDN
                     PKQVGADRIVNAVAAFAKYGGPLVIIDFGTATTFCAVSERSEYLGGSIAPGLKISSEA
                     LFEKTAKLPKVELEEPGHVVCRNTIQSMQAGLVYGHMGMVDYIVRKMKSELHELTESG
                     KEPIVIATGGFADLINEGIDCIDHVDKLLTLEGLSIIYEKNKKAQKIKHNDECHQESP
                     GNQKE"
     gene            complement(3424..3837)
                     /locus_tag="PLM92_01025"
     CDS             complement(3424..3837)
                     /locus_tag="PLM92_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020071777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HPR23759.1"
                     /translation="MKQYNIKVNGVSYTVEVEEAGSVAPAPAPAPAASPAPAASPAPA
                     ASPAPAPAPAAAPPKPAAAPAAGAQTVESPMPGTVLDVKVAPGDQVSEGDLLIVLEAM
                     KMENEIMAPAAGTVDTVPVAKGASVNAGDVLATLK"
     gene            complement(3892..4320)
                     /locus_tag="PLM92_01030"
     CDS             complement(3892..4320)
                     /locus_tag="PLM92_01030"
                     /inference="COORDINATES: protein motif:HMM:TIGR01195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family protein"
                     /protein_id="HPR23760.1"
                     /translation="MSLMEIFADPSKMDSLSFAEKMAGSGVTMLMGLGLTFTVLLLLW
                     ACIAVMGLLMGRNSRKPAKAIKEKAPAKKLTDSPQVLPASAEEVPQNDLIAVITAAVA
                     VAEGKALDDGLVIRKIQRMSGPSTPWHKAGQMECIDSRRV"
     gene            complement(4336..5883)
                     /locus_tag="PLM92_01035"
     CDS             complement(4336..5883)
                     /locus_tag="PLM92_01035"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HPR23761.1"
                     /translation="MDKLEQLREFKAEIAQGGGEKKIEGQHAKGKLTARERLDILFDK
                     GSFVEIDTFVSHRCPNFDMPEKKFHGDGVVTGYGTVDGRLVFAFAQDFTVIGGSLGEY
                     HAEKIVKVQGMALKMGAPIVGLNDSGGARVEEGVNALSGFAKIFKNNTVSSGVIPQIS
                     VIMGPCAGGAVYSPAITDFIFMVDKTSQMFITGPQVIKTVTGEEVSAEDLGGALSNNT
                     KSGNAHFMGSNDEDTLMQVRDLLSYFPSNNMETPPVYPAKDDPNRLIPEFNTIIPENP
                     NKAYDMYEIIKGLADDGIILDVASHFAKNMITCFIRMDGQTVGVIANQPRHEAGCIGI
                     NASDKAARFVRRCDAFNIPMLTLEDVPGFLPGTDQEWGGIIRHGAKLLYAYCEATVPM
                     VTMILRKAYGGAYIGMCNKELGSDMVLAWPTAQVAVMGASGAANIISSVKKEIKESDD
                     PVAKTKEKIEEYEEKFNNPYRAAEMGYVDDVIEPATARKRIISAFDMLATKKSSLPSK
                     KHGNIPL"
     gene            complement(6122..7687)
                     /locus_tag="PLM92_01040"
     CDS             complement(6122..7687)
                     /locus_tag="PLM92_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006001554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HPR23762.1"
                     /translation="MKVTVKDFFELAAFADAKVLAGEKNLGNPVKKVSVLEATGREDV
                     IRYCSAKNQMMLTSFFGIQDDTKAQAEVIKALAEVGNTALVVFHVGEVVKRILPEVIE
                     AAEKAGLPLITMSEKSKFDMGDVINETMSKLFYGESSSFGNDLINNTIFHLLNFEKYN
                     SFQSAVRAAALNNDFQLILLSEDFNPIFYVETRHKTTIDEAIRLGREKEVEKQSTVYT
                     MIDVNGVLTYWGPVSIKDSKYFMFIVDNEDSYSKAEITKLAEIIELAMGMWKYTPERD
                     AKAELIKALRRGNKSLAYTLKEEAEIEGEEIRSVFFARGIENNEAAGHMAEFEKKEEL
                     KVLTINEGDETYGIIIGPQKEGNPCTNLYNTIKTDKSVRIFHVTGIDGTEGAADAYKL
                     IGETWSFVQSVFPYKRVFTKYELVLVSNCINIQLQGGYIKKNYLELLEVFKQMGENKG
                     KQLLDTLETFVLDAGMNSGKTADFMGIHTNTVQYRLKKINELLGVEITGNRVIPGLTI
                     ALALKRLERVVSK"
     gene            complement(7705..9975)
                     /gene="ftsH"
                     /locus_tag="PLM92_01045"
     CDS             complement(7705..9975)
                     /gene="ftsH"
                     /locus_tag="PLM92_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009644240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HPR23763.1"
                     /translation="MKKLSRNIGIYLIIFGLVLAMAWFYNKSPGEDIKDISFSKFTQY
                     VSQEKVEKLYIDENDMALTATLTNGDQVKSVASSLVEVDWLNRTYIYKQMEEGKVILT
                     NAPPKVTPWYISMLPTVIMIIVIVFFWFMIMNQQGGGGKAMSFGKSRAKLQKDSDLRK
                     LTFKDVAGLDEEKEELQEIVDFLKNPKKYNNLGARIPKGILLVGPPGTGKTYISKAAA
                     GEAGVPFFTISGSDFVEMFVGVGASRVRDLFEQAKKNSPCIIFIDEIDAVGRKRGAGL
                     GGGHDEREQTLNQLLVEMDGFAENMGIIILAATNRPDILDPALLRPGRFDRQIVIGVP
                     DVKAREEIFKVHARNKPLADGVEPKVLARRTPGFTPADIENMLNEAALLTARRNGRKI
                     RMDEVEEAITKVIAGPEKKSRVISDEERKLTAFHEAGHAIVAASLPHSDPVHQITIVP
                     RGRAGGFTMVLPKEDRYYGTKTDMEEQIVHLLGGRVAEKLTLDDISTGASNDIMRATE
                     IAKDMVTKYGFSSKLGPVNYSSSDEVFLGKDFSTRQNYSEETASEIDEEIKLIIETAF
                     ARAEEILKKHSKQLKAVADALLELETLDGEQFTELYKGKITAKKLARRVREEEEHIER
                     ANEKEAREMRELIAQQEEEEKEKLDENGIPLLADDDLPSEEELAVYDGDYLNDDEEED
                     LAESAYEDIENKAAQNRKKSSKAGKVKTYDDLQAEKEGEEEGKNPDADEAEGKSKKTD
                     KTKSKGGTAKKKGSGN"
     gene            complement(10072..11535)
                     /gene="tilS"
                     /locus_tag="PLM92_01050"
     CDS             complement(10072..11535)
                     /gene="tilS"
                     /locus_tag="PLM92_01050"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HPR23764.1"
                     /translation="MLEQTVRDTIEKHRLIEQGDHIVLGLSGGPDSMCLFHVLRTVFK
                     EQEIGLTAVHVNHKFRPGAAEEEQEYVEKLCKKAGVDCRSFVFDCSKIAEESHMTGEE
                     AGRKVRYDSFFEVTKELRKKGVPAKKIKTAVAHNMNDQAETVLFRILRGTGTDGLSGM
                     EYKRKDEKGMTVIRPLLDVKRTDIDEYCRSNKLDPRKDKSNEEPLYARNKIRLDLIPY
                     IEENYNPNIKDALIRLCGIAYEDSSFINTEAEKVLKKALVSNKKEKCVIDIREIKDLH
                     SAVIGRVIIKAMGMAGLSSDLTNAHIKQAEELIIKGETSKSTNFPHGYIMKTVYGKAE
                     IYRQEVVETATGTELEMQAEAADGTKLTNKPGLKISILMKEDFTGKKDVAIFDFDMFE
                     EAYKDNEKTFESIVLRTRKPGDFIVLPDVGGRKKIQDLFVDTKVREEKRENVYMVAIN
                     SEILWIPAGITKARYSGNFKVNEDTKRVLVLEIHGSL"
     gene            complement(11535..12392)
                     /locus_tag="PLM92_01055"
     CDS             complement(11535..12392)
                     /locus_tag="PLM92_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005838643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HPR23765.1"
                     /translation="MYFDSHAHLDYEDIKGAGFDENVKAIERSDVDYVMDIGVDLATS
                     KKAAENAGKYSWCYAAIGYHPHEAKGMSDIELEMIKALAKNPKVMAIGEIGLDFHYEY
                     SPKDVQRDVFRRQIELANQLKMPIVIHSREADQEVMDILKEEGAFSEERKSWFPKRPL
                     PVEESEKSRSWLNAAYDARVLLHCFSGSREMGEQYVKLGGTISIAGPVTYKNNRKTTE
                     VVGSVPLEFMLIETDAPFLAPEPKRGKANMSPYIEFTAMRVAAIKGITLDETASKTTE
                     NAKRFFGID"
     gene            complement(12426..14426)
                     /gene="metG"
                     /locus_tag="PLM92_01060"
     CDS             complement(12426..14426)
                     /gene="metG"
                     /locus_tag="PLM92_01060"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006001547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HPR23766.1"
                     /translation="MTQKSEKGKYYISTPIYYPSDNLHIGHTYCTVMADAMARFKRAT
                     GYDVHFLTGTDEHGQKIQTVAQNAGVSPQEYVDKIVGNIKDLWKTMEISYDDFIRTTE
                     PRHIKRVQAIFMKMYEKGDIYKGEYEGLYCTPCESFWTESQLVDGKCPDCGRPVQKAA
                     EEAYFFKISNYADKLLDIFKNQPEFLQPDTRRNEMISFVEQGLEDLCISRSSFDWGIP
                     VPIEENHVIYVWLDALSNYITALGWPDDRELYDKYWPADVHLVGKEIVRFHSIIWPAM
                     LMSIDEPLPKQVLGHGWLLMDDGKMSKSKGNVVDPVELIGRYGIDALKYFLLREYTFG
                     QDGLFTNEVMLRRMNFDLANDLGNLVSRTVAMIEKYNEGKVPGAALTHPEEKDASKEG
                     SDKESHGEYAFHTDRELREIAEGVADRVEKRMDKFEFNLALEEIWVLVRRANKYIDEN
                     TPWILAKDEKDKQRLDTVMHNLAEAIRIVSILIYPFMHTTSGKIRKQLGLWYAEPVWE
                     DAKKFDMMDGEEVKRKDVIFPRLDIEKELKELAELKGGKEDESIPLELKDEIQYEDFD
                     KLDLRVGTILECEKHPKADKLLVFQVKMGTEKRQIISGVAECYKPEDTIGMKVVVVAN
                     LAPRKLRGMPSNGMLLFADNIADGKERFEFVTTVADDGNPVT"
BASE COUNT         3214 a         3683 c         2708 g         4955 t
ORIGIN      
        1 ttgaaggaaa ggtctgatct gttcaagacc ttctttgatt ttctcttcca tttttaaact
       61 cctttttttg atttggctat tgttttcaca taaaatttta tttatgtttc agtctttctc
      121 tttttctttg tctttgtccc ttttctcttt ttctttctct tttttctctt tctctttttc
      181 cgcttttatt accgaagcag gtttggccac atatacagcc gtcactttgc cctccgataa
      241 accgttgata ttaacagacc tttcgggatc tcccttttga tcaaaggtta tgaaaccgga
      301 agctccttca aaattcttgt tgtttcttaa agcttcactt atctttctgc cgttttgtgg
      361 atcacctgct ttttctatgg cattaagtgc caaaagatat gcgtcaaatc ccagagctgt
      421 tgcttccttg ggggcgcttt tgccgtactc tttcctgtaa gcctccagaa aaacaccggt
      481 catttctgtc tgtcttgcat ccgcatcgaa caccgccgac cagaaaactt ccttttcttt
      541 tctgtccttt atttctttta tgaaatcctc atcctgccac aattcagttc ccagaaaaat
      601 aaaaggatac gaaagatctc ttgcctgcct tattatatta agagaatcat ttgcggctcc
      661 gggaaggaaa acgaccctgg caccggtttc ttttattcga taaagctgtc ttccgaaatc
      721 aacatctccc ggagaatatt cctgatagga caaaaccgca tcttcatctt ctgtcagttt
      781 tatcattctg tccgaatatt ccctgctgac ttccatggcc tggtcgtcat cctttggcat
      841 cattacagct gctttttcgt ttttcagact ttctgcagtg aagtccgcca gtgcggtgcc
      901 ctgatccgac tcaacgaaac agaccctgaa ataattgtcc ttttccgatg tgacaagagg
      961 attagtgttt gttacagcta ttgcagggat gttctttttt tcaaaatagc tgcctcccag
     1021 catagaaaaa acgcttccgt agctacccag gatcacagaa ggctcattgg ccaccagatc
     1081 ttttgctgcc gtcttagcca cattcagatc ggatcctgta tctgcatata tcagctcgac
     1141 cttttttccc agaacctcag gataaagctt gttagccagc tccattcccc ttgtttccag
     1201 ttccccttct tctttgaatt ctcctgtaga tggttcgaaa acacctattt ttacggtgtc
     1261 ccttccgcta ctgtctccaa gaaaggcttc tttaaaattg tcatatgtag tacaacctgc
     1321 aaatatgccg actatcatta ttaaagccgt cattagcgca gttgtttttg ctttctttcg
     1381 catatatttc ctcattcata aagttctcca cccaagtcct tccagcagag acactatatg
     1441 attatactat ctttatacta aattttccac tatttgctta agttccgtga ctgtaaccgc
     1501 ttcattgact ttacacctta tcgccgctac tcctctttct cccttgagat accatcccac
     1561 atgttttctc atttctctta ctgccgttct ctcgcctttt tccttttcca gcatttcaag
     1621 gtgttttatt atcattgctt ttttttcctc tgttgaaggt cccggcggga cagactttcc
     1681 ctgccataat gccgatgcct ctttgaatat ccaggggttt cccagggctc ccctggcaat
     1741 cataacaaaa tcacaaccgg tttcttccat catttcattg gcatcctctc cactgaaaat
     1801 atctccgctg cctattaccg gaataacaag agcatccttg acccttgtta ttatttcccg
     1861 gtctgctttg cctccgtagt actgttgtct ggtccttccg tgaactgcca cagccatggc
     1921 tccggcttcc tgcgccgcaa gggctacttc aacggcattt atatgttcgc tgtcccaccc
     1981 ggctcttatc ttaacggtca ccggtttttc tccgggaacc ggatttcctt tggctgaatg
     2041 tgcctcttcc gaaagtcctt tttttgtgcc ttttaccgct gcttcaacta ttttccctgc
     2101 cagggaggga tctttcatca gggcacatcc ttcattgttc tttattactt tgcttaccgg
     2161 acagcccatg tttatatcaa ggatcacatt atctcttttt gacagtattc gtgctgtttc
     2221 ttctataact aaaggttctg caccgaatat ctgaaaggcc agaggttttt cttcgggata
     2281 tatgttaaga agcctttctg tttttttgct attatataaa agtcccttgc cgcttaccat
     2341 ttcagaatac actaaagccg cacccatgga tcttgccagg cttctggtgg gtgcgtctga
     2401 tatccccgcc atgggggcaa agaaaaaagg gttttcaagt tcaatgtttc ctattgacgg
     2461 cattaattat tccttttgat ttcccgggct ttcctgatgg cattcatcgt tatgtttgat
     2521 tttctgggct ttcttgttct tttcatatat tatggaaagt ccctcaaggg tcagaagttt
     2581 gtccacatga tctatgcagt ctataccttc atttatgaga tccgcaaatc cgccggtagc
     2641 tattacgatt ggttctttac cgctttcggt cagttcatgc agttcacttt tcatctttct
     2701 tactatatag tccaccattc ccatatgccc gtaaaccaga ccggcctgca ttgactgtat
     2761 tgtatttctg caaaccacgt gccccggctc ttccagttca accttcggaa gttttgctgt
     2821 tttttcgaaa agagcttccg atgatatttt cagtcccgga gctatgctgc cgcccagata
     2881 ttcggatctt tcgcttacgg cacaaaatgt ggtagcggta ccgaaatcga taataacaag
     2941 gggaccgccg tattttgcaa atgccgcgac tgcattcacg atcctgtcgg cacccacctg
     3001 tttgggattg tcatacttga ttattaatcc ggtttttacc cccggttcaa tgacaattgc
     3061 cctttttctg aaatacttta cagccaaatg ctgtatcgtg aaaagcactg aagggactac
     3121 agtggatacg ataacgtctt ccacatcttt tatgtccaga ttttcgtatg aaaaaagctg
     3181 gttgataatc attccgtact cgtcggcgct cttgttgttg tcactttcaa gtcgccagtt
     3241 ctggatgagt tcaccatctt tgaatacacc aaggactata ttggtgtttc ctacatcaaa
     3301 tgccagtagc atggatattc ctccttcact ctgctttgtg cagtagatat tcaatatgcc
     3361 ggaatacttt ccggttttca aaaaaccagg gcgccgtaaa ccggcgccct tattataaac
     3421 tttttatttc agtgttgcaa gaacgtcccc tgcattaact gatgctccct ttgctaccgg
     3481 tacagtatcc actgttcccg ctgcgggtgc cattatttca ttttccattt tcatggcttc
     3541 aagtactata agcaaatcgc cttcgcttac ctggtctccg ggagccactt ttacatccag
     3601 aactgttccg ggcatgggtg actcaactgt ctgcgctccc gctgccggtg ctgctgcagg
     3661 tttgggtggt gcggccgccg gtgcaggtgc aggagcaggt gatgccgccg gtgcaggtga
     3721 tgctgccggt gcaggtgatg ccgccggtgc aggtgccgga gcaggtgcta cgcttcctgc
     3781 ctcttcaact tctactgtgt atgatactcc gttcactttg atgttatact gtttcattaa
     3841 ttctttctcc ttttcgcaat atataagatt tctttcgatt tatatcacaa ttcaaactct
     3901 tctgctgtct atacattcca tctgtccggc tttatgccag ggtgtggaag gcccggacat
     3961 cctttgtatc ttccttatca ccagaccgtc atctaaagct tttccttcag ctactgctac
     4021 agccgctgtg atcactgcga taaggtcgtt ctgaggcact tcttcagcag atgccggtaa
     4081 cacctggggc gaatctgtca gtttttttgc cggtgctttt tctttgatgg ctttcgcggg
     4141 ttttcttgaa tttcttccca ttaacaggcc catcacagct atgcaagccc acagaagcag
     4201 gagtacagta aatgtgagac ccaatcccat caacatggtc acgccgcttc ctgccatctt
     4261 ttctgcaaaa gacagggaat ccatcttgga gggatctgca aaaatttcca ttaaactcat
     4321 tcagtacacc tccgtttaaa gtggaatatt gccgtgcttc ttggaaggca gagaactctt
     4381 ctttgtagcc agcatatcaa atgcacttat tatcctcttc cttgccgttg ccggctctat
     4441 aacatcgtct acataaccca tttcagctgc tctgtacggg ttgttgaatt tttcttcgta
     4501 ttcttcgatt ttttcttttg ttttggccac gggatcatcg gattccttga tttctttttt
     4561 gaccgaggaa atgatgtttg ctgcgcctga tgctcccatt acagccacct gtgccgttgg
     4621 ccaggccagt accatatccg atcccagttc tttgttgcac attcctatat atgctccgcc
     4681 gtatgctttt cttaatatca tcgttaccat gggaactgtt gcttcacaat atgcataaag
     4741 aagtttcgcc ccgtgtctta ttatgcctcc ccattcctga tcggtgccgg gaaggaaccc
     4801 gggaacatcc tcaagagtca gcatggggat attgaatgca tcgcatcttc ttacgaatct
     4861 tgctgctttg tctgaagcat ttatgccgat acatccggcc tcatgacggg gctgattcgc
     4921 gatcacgccc actgtctgtc cgtccattct tataaagcat gtgatcatgt tttttgcgaa
     4981 atgcgaagcc acatccagga tgatgccgtc atctgccaaa ccctttatta tttcatacat
     5041 atcgtatgct ttgttgggat tctccgggat tatcgtgttg aattccggta tcagtctgtt
     5101 aggatcatct tttgccggat aaacgggagg agtttccatg ttattggaag ggaagtagct
     5161 gagaagatct cttacctgca taagggtatc ttcatcatta ctgcccatga aatgagcgtt
     5221 tccactcttt gtattattgg aaagagctcc gcccagatct tcagcagaaa cttcttcgcc
     5281 ggtaactgtc tttattacct ggggtcccgt tatgaacatc tggcttgttt tgtctaccat
     5341 gaatatgaaa tctgtgatgg ccggtgaata aacagctccg ccggcgcatg gtcccattat
     5401 tactgatatc tgagggataa ctcccgatga taccgtgttg tttttaaata tctttgcaaa
     5461 accggaaaga gcatttacgc cttcttcaac tcttgctccg cccgaatcgt ttaatccaac
     5521 tatgggagct cccattttaa gagccattcc ctgtacctta acgatctttt cggcatgata
     5581 ttcgcccaaa gatcctccga taactgtgaa atcctgtgcg aatgcaaata ccagtctgcc
     5641 gtctacagta ccgtatcctg taactacacc gtcaccgtgg aattttttct cgggcatatc
     5701 gaaattagga catctgtgag aaacaaaagt gtctatttca acaaaactcc ctttgtcgaa
     5761 aagaatatcc agtctttctc tggccgtcag tttgcctttg gcatgctgtc cttctatctt
     5821 tttttctcct ccgccctggg caatctcagc tttgaattcg cgaagttgtt caagcttgtc
     5881 catttatgtt tcctccttgg taaattatgg ggttatttat taatatttca acgctttgaa
     5941 aggttttata ttgttgattt tacaacaaaa cgcctggcaa aacgcaccac tcatactata
     6001 actcacttat ttttattttt caaggaatat ttccttgtat acattattct ttgccaaaat
     6061 tttaacagct ataaatgtgc aaaaaactgc ttttatcaat cggctcaaat catgctgcaa
     6121 actattttga aaccactctt tccagcctct tcaatgccag ggctatggtc agcccgggga
     6181 tcaccctgtt tcctgtgatc tccactccca gcagctcatt gatttttttg agtctgtact
     6241 ggacagtgtt tgtatgtata cccatgaaat cagcagtttt tccactgttc ataccggcat
     6301 ccagaacaaa agtctcaagg gtatccagca gctgtttgcc tttgttttct cccatttgtt
     6361 tgaagacttc cagcagctcc agataattct tttttatgta tcccccctgt agctggatat
     6421 ttatacagtt gctgacaaga accagttcat atttcgtgaa tactcttttg taggggaaca
     6481 cactctgaac aaaggaccag gtctcgccga tgagcttata tgcatcagcc gctccctcgg
     6541 taccatcgat gccggtcaca tggaatatcc ttacactctt gtccgtcttg atggtattgt
     6601 aaagatttgt acagggattt ccttcttttt gaggacctat tattattccg taggtttcgt
     6661 ctccttcgtt tatagtaagg accttgagct cctctttttt ctcgaattcc gccatatgtc
     6721 ctgcagcttc attgttctcg ataccccttg caaaaaatac gctccttatt tcttcccctt
     6781 ctatttccgc ttcttctttt aatgtatagg caagactttt gttgcctctg cgaagggcct
     6841 tgataagttc cgcctttgca tctctttcag gtgtgtactt ccacattccc atggcaagtt
     6901 cgattatttc tgccagtttt gttatttctg ctttagaata tgagtcctca ttatcaacga
     6961 tgaacataaa atatttcgag tcctttatgc tgacgggacc ccagtatgta agcactccgt
     7021 tcacatcgat catcgtgtag actgtggact gcttttcgac ttccttctcc ctgccgagac
     7081 ggatggcctc gtctattgtt gttttatgtc tggtttccac ataaaatatg gggttaaaat
     7141 cttcagaaag cagtatcagc tgaaaatcat tgttcaaagc agccgctctt acagctgact
     7201 ggaagctgtt atacttttca aaattcaata aatggaatat ggtgttgttg ataagatcgt
     7261 ttccgaagct gctgctttct ccgtaaaaaa gcttactcat ggtctcgttt ataacatctc
     7321 ccatatcaaa cttggatttt tcactcattg ttataagagg cagtcccgcc ttttctgcgg
     7381 cttcgatcac ttccggcaat atccttttta ctacttctcc cacatgaaaa acaaccagtg
     7441 cggtgtttcc aacttcagcc aaagctttga ttacttccgc ctgagctttt gtatcatcct
     7501 gtataccgaa aaacgatgtg agcatcatct ggttttttgc agaacaataa cggatcacat
     7561 cttccctgcc ggtggcctca agtacagaaa cttttttgac cggatttccc agattctttt
     7621 caccggccag cacctttgcg tccgcaaaag cagccagttc aaagaaatct tttactgtta
     7681 ccttcatttg gtttccttcc gggtttaatt tccggatccc ttctttttgg cagtgccgcc
     7741 ttttgatttt gtcttatcag tcttcttgct tttcccttcg gcttcatctg catccgggtt
     7801 tttgccttct tcttcgcctt ctttttcagc ttgcagatca tcgtatgttt tgacttttcc
     7861 tgctttactg ctcttcttgc gattttgggc cgccttgttt tctatatctt cgtatgcact
     7921 ttccgcgagg tcttcctctt catcatcatt aagataatcc ccgtcataaa cggccagctc
     7981 ctcttcagaa ggcaaatcat catcggccag aagaggtatc ccgttctcat ccagcttttc
     8041 tttttcttcc tcttcctgtt gggcgataag ttctctcatc tcacgggcct ctttttcgtt
     8101 tgcccgctcg atgtgctcct cttcttccct tactctccgg gccaatttct tggccgttat
     8161 ttttcctttg taaagctctg tgaactgttc gccgtccagg gtctccagct caagcagtgc
     8221 atcagccaca gccttaagct gtttgctgtg cttcttcagt atctcctctg ctcgtgcaaa
     8281 agccgtttct attatcaact ttatctcttc atcaatttcc gaagccgttt cttccgaata
     8341 attctgtctg gtggaaaaat ctttgcccag gaaaacttca tcggaggagc tgtaattaac
     8401 aggtcccagc ttggagctga atccgtattt cgttaccata tccttggcga tttccgtggc
     8461 tctcattatg tcgttgctgg ctccggtgct tatatcatcc aaggtgagtt tctctgccac
     8521 acgtccgccg agaagatgaa ctatctgttc ttccatatct gtttttgttc cgtaatacct
     8581 atcctctttg ggcagtacca ttgtaaatcc gccggccctg cctctgggaa ctatggttat
     8641 ctggtgaacg ggatcgctgt ggggcagtga tgcggcaact atggcatgtc ccgcttcatg
     8701 gaaagcagtg agcttcctct cttcatcact gattactctg ctctttttct ccggtcccgc
     8761 aattaccttt gttattgctt cttccacttc gtccatacgg atcttacggc cgtttcttct
     8821 ggctgtcaga agtgcggctt cattcagcat attctctata tcggcgggag tgaatcccgg
     8881 agttcttctt gccaatactt ttggttccac accatcagcc agaggtttgt tcctggcatg
     8941 gaccttgaaa atttcttctc ttgcctttac atcgggcact cctattacta tctgtctgtc
     9001 aaagcgaccc ggtctgagca gagcagggtc caatatgtca ggtctgtttg tggctgccag
     9061 tattattatt cccatgtttt cggcaaatcc gtccatctca acaaggagct ggttcaaggt
     9121 ctgttctctt tcgtcatgac cgccgcccag gccggcacct ctcttacgtc ccactgcatc
     9181 tatttcatct atgaagataa tgcagggcga attctttttt gcctgttcga aaagatctct
     9241 tactcttgat gctcccacac caacaaacat ttccacgaaa tcagatccgc tgatggtgaa
     9301 aaagggcaca ccggcttctc ccgctgctgc ctttgagata tatgttttac ccgttccggg
     9361 aggtcccacc aaaagtattc ctttcgggat ccttgctccc aggttattgt atttcttggg
     9421 atttttcaga aagtcaacta tttcctgcag ttcttctttt tcttcatcaa gacctgccac
     9481 atccttgaat gtgagttttc tgagatcgct gtctttttga agttttgccc tgctctttcc
     9541 gaaggacatg gctttgcccc cgcctccctg ctgattcatt atcataaacc agaagaacac
     9601 tatcacgatt atcattatga ctgtgggcag catggatatg taccaaggcg tcacctttgg
     9661 aggtgcattg gtcaagatga ccttaccttc ttccatttgc ttatagatat atgtcctgtt
     9721 cagccagtcg acttcaacaa gggatgatgc aacgcttttt acctgatctc cgtttgtaag
     9781 ggtagccgtc agagccatat cgttttcatc gatataaagt ttctctactt tctcctggct
     9841 cacatactgg gtaaattttg aaaaagaaat atcctttatg tcttcgccgg gggatttgtt
     9901 ataaaaccag gccattgcca gcacaagtcc aaaaattatc aaatatatgc ctatgtttct
     9961 cgaaagcttt ttcaatcttt tctctccttt agatttatta tttaatgttt agatttattg
    10021 tttgatatca acaaatttgt attaaaaacc aaaatttatt ttgtaatttt atcataggga
    10081 accatgtatt tcaagtacaa ggaccctttt tgtgtcttcg tttaccttga aatttcctga
    10141 gtaacgggct tttgttattc ccgccgggat ccaaaggatc tcgctattga tcgcaaccat
    10201 ataaacattt tctctttttt cttctctgac tttcgtatcc acaaaaagat cctgtatttt
    10261 ctttcgtccg ccaacatccg gcagcactat gaaatccccg ggtttccttg ttctcaaaac
    10321 tatggattca aaagtttttt cgttatcttt atatgcctct tcaaacatat cgaaatcaaa
    10381 tatggccaca tctttctttc ccgtaaagtc ttctttcata agtatgctta ttttgagccc
    10441 gggtttattt gtgagcttag ttccatccgc cgcttcggcc tgcatttcaa gctctgttcc
    10501 ggtggctgtt tcaacgactt cctgcctgta tatctcagct tttccgtaaa cagtcttcat
    10561 tatatatcca tggggaaaat tcgttgattt tgaagtttct ccttttataa taagctcctc
    10621 cgcctgtttt atgtgagcgt ttgtcagatc gcttgaaagt cccgccatac ccattgcctt
    10681 tattattact ctgcctataa ctgcactatg gagatctttg atctctctta tatcaattac
    10741 gcatttctct tttttattgg ataccaatgc ttttttcaga actttttctg cctcagtgtt
    10801 tatgaaactg ctgtcctcat acgcaatgcc gcaaagtctt ataagtgcat cctttatgtt
    10861 gggattatag ttttcctcaa tgtagggtat caaatccagt cttatcttgt tcctggcata
    10921 aaggggttcc tcattactct tgtcttttct gggatccagc ttatttgatc tgcagtattc
    10981 atctatatct gtccttttca catcaagaag gggtcttatg acagtcatgc ctttttcgtc
    11041 ttttcttttg tattccattc ccgaaagtcc gtcggttccc gtacctctaa gtatcctgaa
    11101 aagaaccgtt tctgcctgat cattcatgtt gtgggcaaca gcagttttta tctttttggc
    11161 cgggacccct ttttttctga gttcttttgt gacttcaaaa aaagaatcgt accggacttt
    11221 acgaccggct tcttcaccgg tcatatggct ttcttcggcg atcttactgc agtcaaaaac
    11281 aaatgacctg cagtcgacac cggccttttt acaaagtttt tcgacatatt cctgttcttc
    11341 ttcagcagct cccggtctga atttatgatt cacatgcacc gccgtgagac ctatctcctg
    11401 ttccttgaaa acagtcctca acacatgaaa aagacacatc gaatccgggc ctcccgaaag
    11461 tcccaaaacg atgtggtctc cttgttcgat caaacggtgc ttttcaattg tatctcttac
    11521 tgtttgttcc agcatcagtc aataccaaaa aatctctttg cattttctgt cgttttgctt
    11581 gccgtttcat caagagttat gccttttatc gccgcaactc tcatggcagt gaattcaata
    11641 tacggagaca tgtttgcttt gcctcttttg ggctcagggg caagaaaagg tgcatcagtt
    11701 tctatgagca tgaattccag cggcacagat ccgaccacct ctgttgtctt gcggttgttt
    11761 ttataagtta cgggaccggc gatggaaatc gtccctccca gtttcacata ctgctctccc
    11821 atttctctgc ttccggaaaa acagtgaagc agaactctgg catcatatgc ggcatttaac
    11881 caactcctgc ttttttcact ttcttcaacc ggcaggggcc ttttgggaaa ccagcttttt
    11941 ctctcttcgg aaaaagctcc ctcttctttt aatatatcca ttacttcctg atcggcttcc
    12001 cgtgagtgga tcacgatggg catcttcagt tgattggcca gttctatctg cctgcggaaa
    12061 acatccctct gtacatcttt cggcgaatat tcataatgaa aatcaagccc tatctctcct
    12121 atggccatga ccttggggtt ttttgcaagg gccttgatca tttccagctc aatatcgctc
    12181 atcccctttg cttcatgggg atgatatccg atggcagcat aacaccatga atacttccct
    12241 gcattttctg cagccttttt ggaagttgcc agatctactc cgatgtccat gacatagtct
    12301 acatcagatc tctctatggc ctttacattt tcatcaaagc ctgcaccttt gatgtcctcg
    12361 taatcaagat gtgcatgtga atcaaaatac atttatatac ccctgcagct ttgttctttt
    12421 tcgcactaag ttaccggatt tccgtcatca gccacagttg tcacaaattc gaatctctct
    12481 tttccgtcag cgatattatc ggcaaataaa agcattccgt tggaaggcat gcccctgagc
    12541 tttctcggag ccagattggc aacaactacc actttcattc ctatggtatc ttcaggctta
    12601 tagcactctg ccacaccact gattatctgc cttttttctg tgcccatttt tacctggaaa
    12661 accagtagtt tatcggcctt cggatgcttt tcgcattcca gaatggttcc cactctaaga
    12721 tccagtttat caaaatcttc atactgtatc tcatctttga gttcgagggg tatactctca
    12781 tcttctttgc cgccctttaa ttctgccagt tccttcagct ctttttcaat gtcaagtctg
    12841 gggaatatga catcctttct cttgacttct tcaccgtcca tcatatcgaa tttcttcgca
    12901 tcttcccaaa ccggttcagc ataccagagc cccagctgtt ttctgatctt gccggaagtc
    12961 gtatgcatga aaggataaat aagaattgac actatcctga ttgcctccgc aagattatgc
    13021 ataactgtgt ccagtctctg cttgtctttt tcatcctttg ccagtatcca gggagtgttt
    13081 tcatcaatat atttgttggc tcttctaaca aggacccata tttcctccag cgccaggttg
    13141 aactcgaact tgtccattct cttttccact ctgtcagcca cgccttcggc tatttccctg
    13201 agttccctgt cggtatgaaa agcatattca ccatgagatt ccttatccga tccttctttt
    13261 gaagcatcct tttcttccgg atgggtcaaa gcagctcccg gaactttgcc ttcgttatat
    13321 ttttctatca tcgccacggt ccttgaaaca agatttccca gatcgttggc aagatcaaaa
    13381 ttcattcttc tgagcatgac ttcattggtg aaaagcccgt cctgaccaaa ggtatattcc
    13441 ctgagcagga aatattttag tgcatctatt ccgtaacggc cgatcagctc tacgggatca
    13501 acaacatttc ccttggattt gctcatctta ccgtcgtcca tcaaaagcca tccgtgcccc
    13561 agcacttgct ttggcagcgg ttcgtctatg gacataagca ttgccggcca tattatggaa
    13621 tggaaccgga cgatttcttt tcccaccaga tgcacatccg ccggccagta cttgtcataa
    13681 agctccctgt cgtcgggcca tcccaatgct gttatgtagt tggaaagtgc atcgagccat
    13741 acatatatca catggttttc ctctatgggt accggtatcc cccaatcaaa tgatgatctt
    13801 gaaatacaaa gatcttcaag gccttgttca acaaaggata tcatttcatt gcgccttgta
    13861 tcaggctgga gaaactccgg ctgatttttg aatatgtcaa gcagcttgtc cgcataattt
    13921 gatattttga agaaatatgc ttcttcagcg gctttttgaa caggcctccc acagtcagga
    13981 catttgccgt ccacaagctg gctttccgtc cagaaagatt cgcagggagt gcaataaagt
    14041 ccttcatatt ctcccttata tatgtctccc ttttcataca ttttcatgaa aatggcctgt
    14101 actcttttta tatgcctggg ttcggtggtc cttataaaat catcataaga tatctccatg
    14161 gttttccaga gatctttgat gttcccgaca attttgtcca cgtattcctg gggcgacacc
    14221 ccggcattct gggccaccgt ctgtatcttc tgcccatgct catccgttcc cgtaagaaaa
    14281 tggacatcgt atcccgtggc tcttttgaac cttgccatgg catctgccat tactgtacag
    14341 tatgtatgac ctatgtgcag gttatcgctg ggataataga tgggagtact gatatagtat
    14401 tttccttttt cgcttttctg tgtcattttc tgttcctttc atgccccctg tcgaatgaga
    14461 cagagttgct gcaggccttt ttccgcccat tgggtgcggt ggactgctaa ccattagaat
    14521 aacacaattt ttgtggtttc gcaaaccccg gtatcaactg
//
LOCUS       DAONHK010000013        13919 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_3458_length_13919_cov_5.088142, whole genome shotgun sequence.
ACCESSION   DAONHK010000013 DAONHK010000000
VERSION     DAONHK010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 13919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13919
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3458_length_13919_cov_5.088142"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..680)
                     /gene="selA"
                     /locus_tag="PLM92_01065"
     CDS             complement(<1..680)
                     /gene="selA"
                     /locus_tag="PLM92_01065"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNASec selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HPR23767.1"
                     /translation="MENNEVLRKLPKIDQVLNDQRLVAVFEEAPREVVVDAVRKTVDA
                     MRKEILAGKETDTENLFERVEKLVKKTGTKSLRRVINATGVIIHTNLGRSVLPESACN
                     NAKFVSTGYSSLEYNLKKGTRGSRHDHLEPLIKRITGAEAAMVVNNNAAATLLCLAAI
                     SGEKETIVSRGELVEIGGSFRIPDIMEISRARLVEVGTTNKTKIDDYKKALNRDSAMI
                     LKVHKSNYA"
     gene            complement(708..804)
                     /locus_tag="PLM92_01070"
     tRNA            complement(708..804)
                     /locus_tag="PLM92_01070"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(768..770),aa:Sec,seq:tca)
     gene            complement(905..3247)
                     /gene="fdhF"
                     /locus_tag="PLM92_01075"
     CDS             complement(905..3247)
                     /gene="fdhF"
                     /locus_tag="PLM92_01075"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(2534..2536),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HPR23768.1"
                     /translation="MAGKKNIYDFKDPDYKGEDYNDDHPYVLRDPNKCIHCGLCVRMC
                     NEVMGIGALAFVNRGFDTVVKPALERPLEESGCVSCGNCVSVCPTGALQARVTQRKPI
                     PLEVELVRTTCPYCGVGCQIDLKVRGNQVVGVQPAFGAANEGILCVKGKFAYDFIGHE
                     DRLKTPYIRKRGKLTPVSWDEALDHIAENISKIREESGPDAIAGFSSARVTNEENYLF
                     QKLFRAGIGTNNVDHCARLUHSSTVAGLAAALGSGAMTNSIPEVMDADAILITGSNTT
                     VGHPVLGARIGQAVREGAKLVVIDPRKIPLAKEADVFLQIKPGTNIAVINGLINVIIK
                     EKLYDREYISKRTEGFEELKETVKDYTPERVAEICGVSAKDVVKAARIYAEAEKSPIY
                     YAMGVTQFATGTAGVMNICNLALVCGKIGKYGCGVNPLRGQNNVQGACDMGCLPGDLT
                     AYQKVANPDALKKFSDFWGVELSNAPGKTITEIMNSICNDDIRCLYIMGENPMISDPD
                     INHVDHALEHCEFLVVQDIFMTETAELADVVLPAACYAEKDGTFSNTERRVQKIRKAV
                     DAPEGVLPDWEILAGLLRRLGVPVEYDSAEDIFNEMRKVTPSYAGITYGRIEELGSLQ
                     WPCLDEEHPGTPVLHTAKFTRGEHALFKPAEYKEPAEQTDSEYNFTFTTGRILFHYHT
                     RTMTGKSDGLNRIAGKSFVEIHPRDAAKYNIKDGEKVTVASRRGQVSIDARVTPDVKE
                     GVLFMPFHFADGPANLLTNSAIDPTAKIPEYKVCAAKIVK"
     gene            complement(3569..5032)
                     /locus_tag="PLM92_01080"
                     /pseudo
     CDS             complement(3569..5032)
                     /locus_tag="PLM92_01080"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
     gene            complement(5048..6643)
                     /locus_tag="PLM92_01085"
     CDS             complement(5048..6643)
                     /locus_tag="PLM92_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007061526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HPR23769.1"
                     /translation="MKVVIGQGSCGIASGAQQTAGEFERYIKEMNIRNVTIEKTGCIG
                     ACFLEPIVDIYSAEEELEGRYVNVFPEKVEEIAKAHLICGKTADTYLISKENEEYLNL
                     QKRVVMRNCGQINPESIDDYTKKGGYKAAEKAVKEMQPENIIDEMEASGLRGRGGAGF
                     PVWKKWKATREQESDVKYVICNGDEGDPGAFIDRALLEGDPHNIIEGMIICAYAVGAS
                     SGIMYVRAEYPLAVERLEKALKQAEGKGFLGKNIFGSDFSFDITLRIGAGAFVCGEET
                     ALIASLEGQQGLPKIRPPYPSKRGYWKKPTNVNNIETLANVPYIIENGGALLASMGTE
                     KSKGTKVFALAGKAKRGGLIEVPMGTTLKEIIFDIAGGIRDGGKFKAAQVGGPSGGCI
                     PEELLHTPVDYESITATGAIIGSGGIIVMDETTCMVDMARYFLDFTAKESCGKCNYCR
                     IGTRRMLEILERITTGTSKDGDIEMLNELAEKVQDGSMCGLGQTAPNPVITTIRYFRN
                     EYKDHIVDRKCTAGVCKFETAQQ"
     gene            complement(6640..7161)
                     /gene="nuoE"
                     /locus_tag="PLM92_01090"
     CDS             complement(6640..7161)
                     /gene="nuoE"
                     /locus_tag="PLM92_01090"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006000951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HPR23770.1"
                     /translation="MTCNPNEYGCGKYPELEPVLDKYADVRGSLITVLQKTQDLYGYI
                     PVDAINHIGERIHVKPSKIYSVATFYAQFRLEPVGKYLIMLCKGTACHVNGTDSIEEA
                     ITEFLGIKDGETTEDGFFTLNNVACLGCCSLAPAMLVRSSEGTEAYGNLTKEKTKKIL
                     NDIKGREAKEVKI"
     gene            complement(7310..8275)
                     /locus_tag="PLM92_01095"
     CDS             complement(7310..8275)
                     /locus_tag="PLM92_01095"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HPR23771.1"
                     /translation="MKKIRIVMLGHGNAGTAFKKLLESKEQEIKEKYNVKCEITAIGT
                     GSRGNLMEPKGISSMEILEKTEYDIMIELTPLAIKTGQPAITHIETALCRGKHVITAN
                     KGPIAWDHRRLRDIAEKNGCMFLYETTVMDGTPVFNMVSENLEMCKVTEVMGILNSTT
                     NYILEEMSKDRPYEEIIREGRERGFIEADASMDIEGHDAAAKLTALMNVLMDAEMTPD
                     DISRKGIEDIVLQDIKSARAKGKVIKLICRGTCSDEKVIGKVAPEEIDKEAIYAGIKG
                     TSSVVSITTDLMGTVHVIEEEPEIEQTAYGIFGDLLKVIKGTNEV"
     gene            complement(8442..11954)
                     /locus_tag="PLM92_01100"
     CDS             complement(8442..11954)
                     /locus_tag="PLM92_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006000947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPR23772.1"
                     /translation="MKKFRMNIDGKEVLGIPGQTILEVAKENEIEIPTFCFDERTKIY
                     GSCGICVCEVEGNPKLVKACATEIAPGMVVRTNTERVIESRKTNLELLLSDHVGDCRP
                     PCVLGCPAQTDCQGYAGLIANGEYTEALYLIKNKIPFPGSLGRVCPHPCETECRRGLI
                     DEPVSIQMLKRFAAENGQTDPEMLMPEPEELTGKRVAVIGGGPMGLSAAFFLRQMGHD
                     VTVFESMPKAGGMLRYGIPEYRLPKEVVDEEVALIEEIGVEIITDTKVGEDIPFETVR
                     SDFDAVLLGIGAWTSTGVGCKGEEAEGVIGGIDFLRKVVRNEPIKLGKKVAVVGGGNT
                     AMDACRTAVRLGADKVYNIYRRTKEEMPADMVEIVEAEEEGVVFKNLTNPIEFKKDKD
                     GHVKEALLQVMQLGEPDESGRRRPVPIEGKTETIEIDNVILAIGQAVDSRIFKGIDKT
                     KKGALAYDEDTYMTSMEGVFAGGDCGNDKISIAVEAIADAKKAATVIDAYLAGEKIRY
                     RKPYVVERDDITEKTFEDRERECRPQISELQVKERKDNFAEVIPDGFSEEDAEKEAKR
                     CLECGCHDYFECKLIDYANQYDVEPKRFAGDKNEIEFEDDHPFIVRDPNKCILCGLCV
                     RVCDEVMGVGALGLVNRGFDTVVKPTLEKPLLESGCVSCGQCVTVCPTGALQERQTVI
                     KEVPLDTVVTETTCAYCSVGCSLELESCGEMLIKANPDKLGPVNAGLACGRGKWGFDC
                     SVLEGKIEDPVVKMGGKARNTDYHDAIIMTAKKAQSIAAKYGKESVAVAVSDRYTNEE
                     AYTVKKLADVMGAKTLCFNNRESGIKKVMGTDASPNTINELLSTDVIIAAGFSTKDNP
                     VIQLKIKQAAENGAKVILINPKGKEQHFEFAEKSVYTKNDLGFLKEMAKYLLESGKTS
                     GAKGFDAFKKSLKDVVPGKEAAEIAETYSKAKKAMIVFTQNFVTTEAAVLIADMALLS
                     GHMGSPRDGILQVKSKNNSQGIIDLGISGGAEVMDGVKALLSFGEDVPAFALKGLEFL
                     MVSDTHMTSSAKKADVVIPGTGSVSTDGTYTNTERRLMPVMAAIDEGIVLSNWEVASE
                     IASVYEVDFGFEDATDISLEMDDFVPSYKYAETGEVTGGVLSPKDPEFVVVGDAKFTD
                     PLKCTDNLMNMITERLPEPAKMTE"
     gene            complement(11971..13719)
                     /locus_tag="PLM92_01105"
     CDS             complement(11971..13719)
                     /locus_tag="PLM92_01105"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HPR23773.1"
                     /translation="MRVLVGEGSCGIATGAKKTAAELEKQIKEKGISAEVEITGCVGN
                     CYLEPIVDVYDDEGRLTRYIKVHPEKVTEIVDNHLAAGRIPDDMTISDQDKEFIEKQK
                     RIVLRNAGRINPEKIEEYIARDGYKAAEKAVKSMTPEDVIEEVKISGLRGRGGAGFPT
                     WFKWNAAKENQGRNKYIVCNADEGDPGAFMDRSVLEGDPHALLEGMIIGGYAMGATEG
                     VIYCRAEYPLAIKRLGIAMEQARGKGFLGKNIFGSDYSFDIRIKAGAGAFVCGEETAL
                     IASLEGERGMPRLKPPFPAAKGYWQEPTNINNVETFANVPWILFNGGASFGAMGTEKS
                     KGTKVFALAGKIKKGGLVEVPMGLPLREIIFGIGGGIKEDKEFKAVQMGGPSGGCIPA
                     SLIDTPVTYEDITKTGAIVGSGGMIVMDESTCMVDMARYFLDFTRKESCGKCNYCRIG
                     TKRMLEILERITEGKGKDGDIELLEELAGKVKDGSMCGLGQTAPNPVLTTIRYFRNEY
                     EDHIYRKKCTARSCKALIKFEITDACVGCTLCAKKCPVDAIAGKVKEKHVIDQDKCVK
                     CGRCYEACKFSAITVE"
     gene            complement(13731..>13919)
                     /locus_tag="PLM92_01110"
     CDS             complement(13731..>13919)
                     /locus_tag="PLM92_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005838096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HPR23774.1"
                     /translation="HLDIKDGETTEDGFFTLNNVACLGCCSLAPVMMVKTADGEETYG
                     NLTKDSVKDVLDSIRERA"
BASE COUNT         3070 a         3634 c         2536 g         4679 t
ORIGIN      
        1 gcatagttgc tcttgtggac cttaaggatc atggcagaat cccggttcaa ggcctttttg
       61 tagtcgtcta ttttcgtttt attggtagtg cccacttcaa caagccgggc tctgcttatt
      121 tccattatat ccggtatcct gaaagagccg cctatctcaa caagttctcc ccttgagact
      181 atggtttctt tttctccgct gatagctgcc agacaaagca atgttgcggc ggcattgtta
      241 ttcactacca tagcggcttc ggctccggtt attcttttga tcaggggttc aagatgatca
      301 tgtcttgagc ctcttgtccc ttttttcaaa ttatattcaa gagaagaata tcctgtagaa
      361 acaaatttcg cattattgca ggcagattca ggcaacacag agcgccccag gtttgtgtgt
      421 attattactc ctgtagcatt tattactctt cttaagcttt ttgtaccggt tttttttacc
      481 agcttttcaa ctctttcaaa aagattttca gtatctgttt ctttccctgc caggatctct
      541 ttacgcattg catctacagt ttttcttact gcatcaacga cgacttctct gggcgcctct
      601 tcaaaaactg caaccaggcg ctgatcattc agcacctggt ctatttttgg aagtttacgc
      661 agaacttcat tattctccat attattcacc cggcctttca tgataaatgg cggggatatg
      721 tgggaatcga acccacccgg gaagcttcta actccccaaa ccggttttga agaccgtcag
      781 gcacaccagc acccatctat ccccctatct aaaaccgcaa ggaatattaa tatgttatca
      841 taatggcatc aaaaaaacaa taacgggacg gcaaagccgt cccgcatttt tgggccttat
      901 ttgattattt gactatcttc gctgcacaaa ctttgtactc gggtatcttt gcagtcgggt
      961 ctattgctga attcgtcaaa aggtttgcgg gtccgtctgc aaaatggaaa ggcatgaaca
     1021 ggaccccctc ctttacatcc ggtgtaactc tcgcatcaat tgagacctgt ccgcgccttg
     1081 atgccacagt gaccttttct ccgtctttga tgttatattt tgctgcatcc cggggatgta
     1141 tttcaacaaa ggatttgcct gctatcctgt tgagtccatc actctttcct gtcattgttc
     1201 ttgtgtggta atggaaaagt atcctgcctg tggtgaatgt gaagttatat tcggaatccg
     1261 tttgttcagc cggctctttg tattctgcag gcttgaacag ggcgtgttct cctctggtga
     1321 atttcgccgt atgcaaaaca ggggtccccg gatgttcctc gtcgagacac ggccactgca
     1381 gtgatcccag ttcttcgatc ctgccataag ttatgcctgc atatgaaggc gtcacttttc
     1441 tcatctcatt gaaaatatct tcggcagaat catactctac gggaacgccc agtctccgaa
     1501 gaagtcctgc aagtatctcc cagtcaggta aaacaccctc cggtgcatcc acagccttcc
     1561 ttattttctg tactcttctt tcggtatttg agaaagtacc gtctttttcc gcatagcagg
     1621 ctgcaggaag aacgacatcc gccagttcag ctgtttctgt catgaatatg tcctgtacca
     1681 ccaggaactc gcaatgctcg agagcatgat ccacatggtt tatatccggg tcagatatca
     1741 tcggattctc tcccattatg taaagacatc tgatatcgtc attgcatatc gagttcatta
     1801 tttcggttat tgtcttcccg ggtgcattgg aaagttcaac gccccagaag tcactgaatt
     1861 ttttcagtgc gtccgggttt gcgactttct gatatgctgt cagatctcca ggcagacatc
     1921 ccatatcaca ggctccctgc acgttgttct gacctcttaa tggatttacc ccacatccgt
     1981 atttgcctat tttgccgcat accagtgcca gattgcatat attcattacg cccgccgtac
     2041 ccgttgcgaa ctgggtcaca cccatggcat agtaaattgg tgatttttcg gcttctgcat
     2101 atatcctggc tgcttttacc acatcctttg cagagactcc gcatatttct gccacccttt
     2161 caggtgtgta atctttgact gtttccttga gttcctcaaa tccttcggtc cttttgctta
     2221 tatattccct gtcatacagt ttttctttta tgatcacatt tatgagacca ttaataaccg
     2281 caatatttgt tcccggtttg atctgcaaaa atacatctgc ctcttttgca aggggtattt
     2341 tcctgggatc tattactaca agctttgctc cttcccttac ggcctgtcct attctggctc
     2401 ccagaacggg atggcctacc gtggtgttgg accctgtgat aagtatcgca tcagcatcca
     2461 taacttccgg aattgaattt gtcatggcgc ctgaaccaag tgctgccgca agacctgcga
     2521 ctgtggagct gtgtcacaac cgggcgcagt ggtccacatt gttggtacct attcctgcgc
     2581 ggaaaagttt ctggaacaaa taattttctt catttgttac tcttgcactg gaaaaacctg
     2641 caatcgcatc cggtcccgat tcttccctta tcttcgaaat attctctgct atgtggtcaa
     2701 gtgcctcatc ccagcttacc ggagtcaatt tacctctttt tcttatatat ggtgtcttca
     2761 atctgtcttc atgacctatg aaatcatatg cgaacttgcc cttgacacaa agtattcctt
     2821 cattggcagc cccaaaggca ggttgcacac ctaccacctg attacctctc acctttaagt
     2881 ctatctgaca tcccacacca cagtatgggc atgttgtcct tacgagctcc acttcaaggg
     2941 gtatgggttt tctctgggtc actcttgcct gcagtgctcc agtgggacat acgctgacgc
     3001 agtttccgca ggaaacacag ccggattctt caaggggccg ttcgagagcg ggttttacta
     3061 ctgtatcaaa tcccctgttc acaaaggcca aagctcctat tcccattact tcattacaca
     3121 tcctcacgca gagcccgcaa tgtatgcact tgttggggtc tctaaggaca taagggtgat
     3181 catcgttgta gtcttctcct ttgtaatcgg gatccttgaa atcatagata tttttctttc
     3241 ctgccattct ggcatgtccg ctggcaccga aaagatttgt atactctata agtctacagg
     3301 taaagtaatt cccgcatccg cattccaggc agcgcttcgc ttcttctatg gcttcctctt
     3361 catcatatcc gtatacgact tccttgaagt tgccttttcg cttgtttgct tccatctggc
     3421 ccattctctt gcggaatttt ctttctctgt cttcgaaggc ctcttcatca aagtcatcac
     3481 gtatatcaac ataaggcatc tgatgtttga ttttttcccc gtgcagatat gcatctatta
     3541 cttctgctga cttattggca tctgccatgg cttctactgc tatggaaatt ttatcattgc
     3601 cgcagtctcc gcctgcaaaa acgccctctt cacttgtcat gtaggtgtcc ggatcatatg
     3661 cgataccttt tcttctggta agctcgatat tctttatgcc ttctgaattt acagcctgac
     3721 ctatggccag aagaacataa tccacatcca gggtctctgt tttcccttct acaggaacag
     3781 gttttcttct tccggattca tcaggttcac ccagctccat cacctgaagg tgtatctgtt
     3841 ttacccttcc ttcttccccg ggtattattt cgatgggatt tgtaaggttt ttgaaaataa
     3901 caccttcttc ttcaccttcc agtatttcta tttcatcagc cggcatttcc gcctttgttc
     3961 ttctgtaaat attatatgca ttttttgcac ccagtcttac ggcggtcctg cagcagtcca
     4021 ttgctgtgtt tcctccgcct actatggcca cattctttcc ctttagatcc ggcggtgttt
     4081 tatttatgac tgactgaaga aaatcgatgc ctccgataac accttctgta tcttctccct
     4141 tacaaccggt acctgtggac acccatgctc ctatagctac cagtaccgca tcatatttct
     4201 ttttgagttc ttcaaaggtt atatctcttc ctatccttgt attaactttg atctcaacac
     4261 cggttttttc tataagtttt acttcctgat caagcacttc ttcctttgga agtctgtact
     4321 gggggatacc gtatctgagc attccgccgc aatatggcat ttgttcatat accgttacgg
     4381 catgtccctt ctggcgaaga aaatatgcag ccgaaagtcc catgggacct ccgcctatta
     4441 cagcgacttt tttgcctgtg tcaggttcac attcgggtat aaagggatta ccgctttctg
     4501 catccacatc tgcggcaaaa cgcttgagcc atgcaatgga tatgggttcc tccacaagac
     4561 ctcttcgaca gtttgtttca caggggtggg gacataccct tccaatggcc cccggcagag
     4621 gtatcttttc ttttataagt tttatagctt cttcaaactg tccgttggct ataagtccaa
     4681 catatccctg acagtctgtc cctgccggac aatttaagga gcatggtcct ttgcagtctc
     4741 catgatgatc ggacatcaaa agctccatat ttgtttttct ggattctttg atcctttctg
     4801 tatttgttct gacggccata tcgggctcta tttcagtgct gcatgatttg agaagctttg
     4861 gatttcccgc cacctcaaca acacatatac cgcaggctcc gtatgcttcc actctcttat
     4921 cataacaaag agacggaatg aaaatatcat tctcttttgc gacctccaat atagtttgcc
     4981 ccgggcttcc gaagcattcc tttccgtcga tcaaaactct gattttatcc attttagaaa
     5041 ttctccttta ctgctgcgca gtttcaaact tgcagactcc cgccgtacat ttgcgatcta
     5101 caatatgatc tttatactca tttctgaaat acctgatcgt tgttataaca gggtttggtg
     5161 ccgtctgacc caaaccgcac attgaaccgt cctgaacctt ttcagccagc tcgttgagca
     5221 tttctatatc accgtctttg cttgtgcctg ttgttatcct ctccagtatt tcaagcatac
     5281 gtcttgtccc tatacggcag tagttacatt ttccgcagga ctcctttgct gtaaaatcaa
     5341 gaaaatatct ggccatatca accatacatg ttgtttcatc cattacgatt atgccaccgg
     5401 atccgattat tgctcccgtt gcagttatgc tttcatagtc aacgggtgtg tgcagcagtt
     5461 cttcaggtat acatcctccc gaaggacccc cgacctgggc agccttgaat ttgcctccgt
     5521 ctcttattcc tccggctata tcgaatatta tttcttttaa tgtggttccc atgggaacct
     5581 cgatcagccc tcctcttttg gcctttcccg ccagagcaaa aacctttgtt cctttgcttt
     5641 tttcggttcc catactggcc aataaagctc ctccgttttc tatgatataa ggaacattgg
     5701 caagtgtttc gatattattg acattagtcg gttttttcca gtaacctctt tttgagggat
     5761 atggaggcct tatttttgga agtccctgct gtccttcaag tgatgcaata agtgcagtct
     5821 cttctccgca tacaaatgct cctgctccta tcctcagagt tatatcaaag ctaaaatcac
     5881 tgccgaaaat atttttgccc aaaaaccctt ttccctcggc ctgcttcagg gctttttcaa
     5941 gcctttccac agcaagggga tattctgctc tcacatacat tatccccgag gatgctccta
     6001 cggcataggc acatattatc atgccttcaa ttatgttatg aggatctcct tccaggagag
     6061 ctctgtcaat aaatgccccg ggatcccctt catcaccgtt gcatattaca tatttcacat
     6121 cactttcctg ctctctggtg gctttccatt ttttccatac gggaaatcct gcgccgcccc
     6181 tgcctctgag tcccgatgct tccatttcat cgatgatatt ttcgggttgc atttctttga
     6241 cggctttttc cgctgcttta tagccgcctt tttttgtgta gtcatctata ctttcggggt
     6301 ttatctgccc gcaattcctc ataacgactc ttttttgcag attcagatat tcctcgtttt
     6361 ccttgcttat aagatatgta tctgctgttt tgccgcagat aaggtgagct tttgcaatct
     6421 cttctacttt ttccggaaac acattgacat atctgccttc cagttcttct tcagctgaat
     6481 atatgtcaac tatgggttca agaaaacacg cccctatgca gcctgttttt tcaatggtca
     6541 cattccggat gttcatttct ttgatatatc tttcgaactc tccggccgtt tgctgggcgc
     6601 cggaagctat accacagctg ccttgtccaa taacgacttt catattttta cctccttagc
     6661 ttcccttccc tttatatcat tgagtatctt tttagttttt tctttggtga gattcccgta
     6721 ggcctctgtt ccttcactgc ttctgaccag cattgccggt gccagagaac aacatcccag
     6781 gcatgccaca ttgttcagag taaaaaaacc gtcttctgtg gtttctccgt cttttatccc
     6841 aaggaactcg gttattgctt cttctatact gtcagtaccg ttcacatgac atgcggtgcc
     6901 tttacacagc attatcagat atttgcccac cggttccagc ctgaactgag cataaaaagt
     6961 agccacactg tatattttag aaggttttac gtggatcctc tctcctatat gatttattgc
     7021 atctacaggt atatatccgt aaagatcctg tgttttctgc aaaactgtga tcaggcttcc
     7081 tctcacatca gcatacttat caagcacagg ctcaagttcc ggatattttc cgcaaccgta
     7141 ctcatttggg ttacaagtca tgtcaatcct cctgttccct aaaaaaacca gtcgaaaccg
     7201 gttgtacttt caagtattaa ttatatcaat agggcaatag aaaaacaagt ataaggcatg
     7261 ttttgggtat aatatttata aataatatct attaaaaaat agattatcct tatacctcgt
     7321 tagtcccctt tataactttt aggagatccc cgaatattcc gtatgctgtc tgttctattt
     7381 ccggttcctc ttcgatcaca tgaactgtgc ccataagatc agtagtaatt gaaacaacag
     7441 aagacgttcc cttgattccg gcatatatcg cctctttgtc tatttcttcc ggtgccactt
     7501 tgcctatcac cttttcatct gaacatgtac ctctgcagat cagttttatt actttcccct
     7561 ttgctcttgc tgattttata tcttgaagta caatatcttc aatgcctttt ctgctgatat
     7621 catcgggagt catttcggca tccatcagca cattcatcaa agcagtaagt tttgctgcag
     7681 catcatgccc ctctatatcc attgaagcat cagcttctat aaaacctctt tccctgcctt
     7741 ctcttattat ttcttcatag ggtctgtcct ttgacatctc ctcaaggata taatttgtag
     7801 tgctgttcaa tatccccatc acctctgtta ctttgcacat ttcaagattc tcgctgacca
     7861 tgttgaacac cggcgtaccg tccattacag tggtttcata taaaaacata catccgtttt
     7921 tttctgcaat atcccttaat cttctgtgat cccaggcaat cggtcctttg ttggcagtta
     7981 tcacatgttt acctctgcaa agagcggttt caatatgagt tatggccggc tgtcctgttt
     8041 ttattgccag cggtgtaagc tcgatcatta tgtcatactc tgtcttttcg agtatttcca
     8101 ttgaacttat accctttggc tccataagat tccctctgct tccggtgccg atggcagtta
     8161 tttcacattt gacattatac ttttctttta tttcctgttc cttgctttca agaagtttct
     8221 tgaatgcggt gccggcattc ccgtgcccca gcataactat ccttattttt ttcatggttt
     8281 tgtcctccgt ctttgactta tgacgctaac atttgatatg attatatcct tttcctatac
     8341 tattataaag catatccttg aacatacttt cataagaaaa gagccttccc cggccctgaa
     8401 ggtctcggga agactccttt aactgttttt ttatttttgg gtcactctgt catttttgcc
     8461 ggttcaggca gtctttctgt tatcatgttc ataagattat ccgtacattt gagaggatct
     8521 gtgaattttg catctcccac tacgacaaat tccggatctt tcggtgaaag gactccgcct
     8581 gttacctctc cggtctccgc atacttatat gacggtacga aatcatccat ttcaagggat
     8641 atatccgtgg catcctcaaa tccgaaatcc acttcatata cagatgctat ttcagatgcc
     8701 acttcccagt tggaaagcac aattccctca tcaatggcag ccatgacagg catcagtctt
     8761 ctttctgtgt ttgtataggt tccgtccgtt gaaacagaac cggtaccggg tataacaaca
     8821 tctgcttttt ttgcggaaga ggtcatatgt gtatctgata ccataaggaa ttccagaccc
     8881 ttcaatgcaa atgcaggaac atcttcgcca aatgacagca aggcctttac tccatccatc
     8941 acttcggctc cgccactgat accaaggtct attattccct gagaattgtt ttttgatttc
     9001 acctgcagta tgccgtctct tggtgatccc atgtgccccg aaagaagtgc catatccgca
     9061 atgagaacag cagcctcggt cgtaacaaag ttctgggtaa atactatcat tgcttttttt
     9121 gccttggaat atgtttctgc gatttctgca gcctcttttc cgggcaccac atccttgagg
     9181 ctttttttga atgcatcaaa tcccttggct ccggaagttt taccgctttc taacaggtat
     9241 tttgccatct ctttaaggaa gccaaggtca ttttttgtat aaacggattt ctcggcaaac
     9301 tcaaagtgct gttctttgcc tttgggattt atcaatatga cttttgcgcc gttttctgca
     9361 gcctgtttta ttttcagttg gatcacagga ttgtctttcg tgctgaaccc ggccgctatt
     9421 atgacatcag tggaaagcag ttcgttgatg gtatttggag acgcatctgt tcccataact
     9481 ttttttatcc cgctttctct gttattgaag catagtgttt ttgctcccat aacatcagcc
     9541 agttttttta ctgtataagc ttcctcattg gtatatctgt cggaaacagc aaccgcaact
     9601 gattctttgc catatttcgc ggctatggat tgggcctttt ttgcagtcat gattattgca
     9661 tcatgataat ccgtgtttct ggctttcccg cccattttca cgacaggatc ttctattttg
     9721 ccttcaagta cagaacagtc gaatccccat tttccccttc cgcatgccag accggcattg
     9781 accggcccca gtttgtcggg atttgctttt atgagcattt ctccgcatga ctcaagttca
     9841 aggctgcatc ccactgagca gtatgcacat gtggtttctg ttacgaccgt atccagaggc
     9901 acttccttta tgactgtctg tctttcctgg agagctcctg tgggacatac tgtaacacac
     9961 tgcccgcacg aaacacatcc cgactcaaga agtggctttt cgagagtggg tttgacaaca
    10021 gtatcaaatc ctctgttcac aagtccaagg gcgcctactc ccatgacttc atcacatacc
    10081 cttacgcaaa gaccgcaaag aatacacttg ttgggatccc ttactatgaa gggatgatcg
    10141 tcttcaaatt cgatctcgtt cttatctcct gcgaatcttt ttggttccac atcatactga
    10201 ttggcataat ctatgagttt gcactcgaaa taatcatgac atccgcattc aagacatctt
    10261 tttgcttctt tttctgcatc ctcttctgag aaaccatctg gaatgacctc ggcaaaatta
    10321 tcttttcttt cttttacctg aagctctgag atctgtggtc tgcattctct ttccctgtcc
    10381 tcgaaagttt tttctgttat gtcgtccctt tcgacaacat agggtttcct gtatcttatc
    10441 ttctctccgg ccagataagc atctattacc gttgctgctt tttttgcatc tgcgattgct
    10501 tcgaccgcta tggatatctt gtcattacca cagtctcctc cggcgaaaac gccttccatg
    10561 cttgtcatat aagtgtcttc atcataggcc agtgcgcctt tttttgtctt gtcaataccc
    10621 ttgaaaattc tgctgtccac agcctgacct atggccagta taacgttatc aatttcaatt
    10681 gtttctgtct ttccttcaat gggcaccggc cgtcttcttc ccgattcatc cggttcaccc
    10741 agctgcatta cctgcaaaag agcttctttt acatgtccgt ctttgtcttt tttgaattct
    10801 atgggattgg tcagattctt aaaaacaaca ccctcttcct cagcctcaac tatctcgacc
    10861 atgtcggcgg gcatctcttc ttttgtcctt ctgtaaatat tgtacacctt gtccgcaccc
    10921 agccttacag ctgtcctgca tgcatccatg gcagtattcc ccccacctac cacagcaact
    10981 tttttgccaa gctttatggg ttcgttcctt actacttttc tcaggaaatc tatgccgcct
    11041 atgacgcctt cggcctcttc acctttacat cccacaccgg tagatgtcca tgcacctatt
    11101 cccagaagga cggcatcaaa atcggaacgt acagtttcaa aaggtatgtc ctctccgact
    11161 tttgtatctg ttattatctc tactccgatc tcttctatga gtgccacctc ttcatccact
    11221 acttccttgg gaagcctgta ttccggtata ccgtatctga gcattccgcc tgcctttggc
    11281 atggactcga atactgtcac atcatgcccc atctgcctca ggaagaatgc agccgaaagc
    11341 cccatgggtc ctccgcctat tacagcaacc cttttgcctg tcagttcttc cggttcaggc
    11401 ataagcattt ccggatccgt ctgtccgttt tctgctgcaa atctttttag catctgtatg
    11461 gaaacgggct catcaataag gcccctacgg cactccgtct cacatggatg gggacatact
    11521 ctgcccagac ttcccgggaa gggtatcttg ttttttatca ggtacaaagc ttcggtatat
    11581 tccccgtttg caataagtcc ggcataaccc tgacagtccg tctgtgccgg acaaccaagc
    11641 acgcaaggcg gtctgcagtc tcccacatgg tcggaaagaa gcagttcaag atttgttttc
    11701 ctcgactcta ttactctttc tgtattggtc cttaccacca tccccggagc tatttcggtt
    11761 gcgcatgcct taaccagttt agggttgcct tcaacttcgc atacacatat accgcatgat
    11821 ccgtatatct ttgttctctc atcgaaacaa aatgtaggta tttcaatttc gttttccttt
    11881 gcgacctcaa gtattgtctg tccgggaatg cccagcactt cttttccgtc tatattcatt
    11941 ctgaattttt tcattgtgct cctgccccct ttactctact gttattgcgc tgaacttgca
    12001 ggcctcataa catctgccgc atttaacgca tttatcctga tcaataacgt gtttttcttt
    12061 tacctttcct gctattgcat ctacagggca tttttttgca caaagggtgc aacccacaca
    12121 tgcatcggtt atttcaaatt ttatgagagc cttgcaggat cttgcagtac atttttttct
    12181 gtatatatga tcttcatatt catttctgaa ataccttatg gttgtaagaa cgggatttgg
    12241 tgctgtctga cccagtccgc acatggaccc gtctttaact ttgccggcca gttcctcaag
    12301 aagttctatg tccccgtctt ttcctttccc ttcagttatt ctttcaagta tttccagcat
    12361 acgcttggta cctatcctgc agtaattgca cttgccacag gattctttcc ttgtgaaatc
    12421 cagaaaatat ctggccatat cgaccataca agtgctttca tccattacta tcattccgcc
    12481 tgatccgact attgcacctg tctttgttat gtcctcatat gttacaggtg tgtctataag
    12541 actggcgggt atgcatccgc ccgaagggcc tcccatctgc acggctttga attctttatc
    12601 ttctttgatt cctccgccta taccgaaaat tatttctctg agaggaagac ccatggggac
    12661 ttccacaagt ccgccttttt ttatctttcc tgccagtgca aatacttttg tacctttgct
    12721 cttttctgtt cccatagctc cgaaagaggc accgccgtta aaaagtatcc atggaacatt
    12781 tgcaaatgtc tccacattat ttatattggt gggctcctgc cagtatccct ttgcagccgg
    12841 gaaaggaggt ttgagtctgg gcatccctct ttctccttca agagatgcaa taagtgcagt
    12901 ttcttctcca catacaaaag ctcccgcacc tgccttgatc cttatatcaa aactgtaatc
    12961 gctgccgaaa atatttttgc ccagaaatcc ttttcctctg gcctgttcca tggctatgcc
    13021 aagtcttttt attgccagag gatattcggc acgacagtat ataactcctt ctgtggctcc
    13081 cattgcgtag ccgcctatta tcattccttc aagcagggca tgggggtcac cttccaaaac
    13141 agatctgtcc ataaatgctc ccgggtctcc ctcgtcggca ttgcatacta tatacttgtt
    13201 cctgccctgg ttttcctttg ctgcattcca tttgaaccag gttggaaagc cggctccgcc
    13261 tctgcctctt aaacccgata tctttacttc ctctatgaca tcctccggtg tcatggattt
    13321 taccgccttc tctgcagctt tatatccgtc cctggctatg tactcttcta ttttttccgg
    13381 atttatcctt cctgcatttc taaggacgat ccgtttttgt ttttctataa attccttgtc
    13441 ctgatcactg atagtcatat catccggtat cctgccggct gccagatgat tatcaactat
    13501 ttcggttact ttttcaggat gaacttttat gtatcttgtc agtctgcctt catcatcata
    13561 aacgtcaact atgggctcca aataacagtt gcccacacag cctgtgattt caacttcagc
    13621 tgaaatgcct ttttccttta tttgtttttc cagttctgca gcggtttttt ttgctccggt
    13681 agcgataccg cagctgcctt ctccaactaa tactctcatc tattcacctc ctatgccctt
    13741 tctctgatac tgtcgagcac atcttttact gaatccttgg tcagattccc atatgtttct
    13801 tcaccatcag cagtttttac catcatgacc ggagccagtg agcagcatcc gagacaagcc
    13861 acattattca aagtgaaaaa tccgtcctct gttgtttccc cgtcttttat gtcgagatg
//
LOCUS       DAONHK010000014        13499 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_3584_length_13499_cov_4.672121, whole genome shotgun sequence.
ACCESSION   DAONHK010000014 DAONHK010000000
VERSION     DAONHK010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 13499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13499
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3584_length_13499_cov_4.672121"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            224..2920
                     /locus_tag="PLM92_01115"
     CDS             224..2920
                     /locus_tag="PLM92_01115"
                     /inference="COORDINATES: protein motif:HMM:NF019217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GLUG motif-containing protein"
                     /protein_id="HPR23775.1"
                     /translation="MRSSKKTYLNRKVPALIITLSMIAGLLGNMGYAKAEETVWDGTS
                     DVSWYTVDKDALSYEIATAEDLAGIAEIVNGTAEDEYGQIEKNNFEGVTFTLTADIVL
                     NTDPIPGRDDTLKEWTPIGGGANKEVFRGTFDGQQYSIKNIYINADGSWGEDENRNIG
                     LFGYIGGEAAVRDLTIKDGWIFGRRAVGAVVGRTEDYNVSLGHGAIIENCHNEGTEVT
                     STDKTGVGGIVGAGYNMALISNCTNSGDIKADGQYDAGGIVGDCEGIIQNCSNSGKVS
                     SITGNAGGIVGYNQHFDTYVPQTINCYNTGEVSSNGYAGGIIGYQEGGSRNCYNVGAL
                     SGDGNKGGVIGDLNSRYDNENLYFLNSEDRDIPGVARQQAGTTGNVETTEKTEDEMKG
                     EPFPGLLNGDDTDYGEGDAFRYEAGDYPVFPAYNDGREKVVSDIKITVPPSTVYYKTG
                     DVLSLSGMKVMISYADGSAATEITYENNTPAGILTTLSDSDTSVAGTKTADIRYGYRT
                     ANQALMVCDVNIVYGLIADLGEIKWDSGGKLVEARSAYAVLEGDEGTNKGVENYNALL
                     TAENSYKNTEGQYGIAYIGFGEYIDNIRDEYKESGIAELNDIKSETLGIIGSQEIEVA
                     GIWEKLKEAESGITALSKYLKPSLSFAQIAVETQTYTGSALMPEIVVTYSGRTLVRNT
                     DYEVAYSNNIKARASGKATVTGTGDYRGTTSATFYIVQAANKIYCKDRIDKTYGATAF
                     SLGATAREGALISYASGNTSVAAVSAGGKITIKGTGIASITVTAKSTANYKTTSKTIT
                     IYVKPKKARIYKATSPKKRTLKATWKRDKKATGYQVICATNKRFTKNKKTALVKKSKT
                     TSKTIKKLKKGKKYYVKVRAYKTARGQKIYGSYSKVKTVRVR"
     gene            2976..4166
                     /locus_tag="PLM92_01120"
     CDS             2976..4166
                     /locus_tag="PLM92_01120"
                     /inference="COORDINATES: protein motif:HMM:NF014427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HPR23776.1"
                     /translation="MKEIMYKNKKIILIIAAGLVLLFSVMSFSVTTADASASKLDIYV
                     GYYGGPYYLKKSYSVNDMYSLPTHRRVYSFIDAAPRPCLDCAIGVDMEDLISDAKIDM
                     NSIETFYFYTSDSGDTYYTSFPKSELIDTVRYYYPELYRHFDKLQGGTEGAEEGAVAV
                     PAMLAISDDFIKMEAGDIFVENYDDQSSENRFRLLFGQTDTVTRNATRSAKYVGRINV
                     MLGGYPTITAPNSYLDMKVGSKKNISITIDAADILLGKKIKESITWTSSDESVATVDR
                     NGMVTVTGEGEAVITAKYKDQSVEVTINGEKGSAAGYGGGNGGGIGSISEVSLGEGDG
                     IDVETSGGSGQAWRVYEMDEKAEELPEIKKNNDLIPVIIMIMILLISGIAGRTLKFYR
                     EVRG"
     gene            4169..4816
                     /locus_tag="PLM92_01125"
     CDS             4169..4816
                     /locus_tag="PLM92_01125"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HPR23777.1"
                     /translation="MISQGLSDALRAVAEAALTPVVIILLVLIAVTIILLGTLIAEFF
                     TERRHLKVKMPVLINQIRAAEKSPEEIIAGSGLIKKQKETLKELVLQSELTNTMREAL
                     AVRLLAETRARYDGIVNITNVIAKIGPMLGLLGTLIPLGPGIIALGRGDTYTLSTSLL
                     TAFDTTVAGLICAAIALIVSVIRKKWYADYMSMLETLMECILDKEKKDDKALWNR"
     gene            4791..5105
                     /locus_tag="PLM92_01130"
     CDS             4791..5105
                     /locus_tag="PLM92_01130"
                     /inference="COORDINATES: protein motif:HMM:NF021440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2149 domain-containing protein"
                     /protein_id="HPR23778.1"
                     /translation="MIRRFGTGKLKGEGIAKEDINPMEGIANLADVMLILAVGIMLAL
                     VMHWNVDISTKEVTQKQEIKDMEDQEISEKIESQKGFEELGTVYKDPETGKMYMVVRE
                     EK"
     gene            5102..7198
                     /locus_tag="PLM92_01135"
     CDS             5102..7198
                     /locus_tag="PLM92_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPR23779.1"
                     /translation="MNRKKLIIIIALVAVVLAGVGFWAFSSGLDKGAAHTNYSAEENY
                     GKGNYVFSTVTFTGSGMERETVLSVKEIEQLAYDNDKDLGYEEKYSLLTSGSIFSEST
                     FTGIKLYETLLYLGMDKSSSDDTRITVVSSDGYTMVMTLGEIRSSKYCRFGSASNPKV
                     EEEGLPVLLSFGSGGLPLVGPTGSEDTGKVMTEKDGFEEEAQNTGGPVKLTIGQKTCD
                     DFNAPQNAKWVKEVIVGDPYDHTFHAGESGKEKALNVSVFDADSGNKKISSKEYTFDE
                     IEKFASRKSENTQKNYYGEKDFYEGAGIWEFLREETEVAAREGTVKFNFSDGKSERVS
                     LSYLRDLNGDHENYITEKEGLYITCVKPAFGYSKNGGPTELGELFALLPKDGKEKKKS
                     TVKKCLSVDVYVGDDYFGSKNPYSNKGIEIEGSGMEEDVYIYIEEIEKQIDLAASSGD
                     YKGVSLIGLLEEKGLAVDAQKAVIESSSGSTEISISTLKKEERNTILATRKRGKALDE
                     EEGPLALRGRDELNNVKKIIVYAKDGKWSHSYKGYKKYLGRKVKITGSEVADDRTYTL
                     KELESLGDGYTVKNAFGAGGGTNAFCGVVLKKLVNDNLKEGIKRPSRITVAGKDGYSI
                     SISVKDVYNGIESKYQPGETRDVILAYSIDGMPMVPSEKSDGYSGGNAFGPVRLVVEN
                     QTSKWVKNVNRIIIGE"
     gene            7201..7785
                     /locus_tag="PLM92_01140"
     CDS             7201..7785
                     /locus_tag="PLM92_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPR23780.1"
                     /translation="MKKNKIKKILAASLCIIIALAFSACGGSESNESDNNGSGKEKSE
                     AALVITGYNDDKTEFSLEDIEEMGMEKYIFSGRDKKADNGRQIQEFTGVPLKEVLEAA
                     GYGATGEIMKVICSDGYSREYEIDDLFGLYAYADEEDTEGVKVDPILAIEEKDGEKII
                     RLVYGQEDYDAPETKDFNMQGWASWIEKIEVGNE"
     gene            7778..8935
                     /locus_tag="PLM92_01145"
     CDS             7778..8935
                     /locus_tag="PLM92_01145"
                     /inference="COORDINATES: protein motif:HMM:NF021348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HPR23781.1"
                     /translation="MNRLLQKFTVYDLVIIAIMAAIGIVIKPVVVPLAHIVTGPLMIP
                     SGALSGGLYMMWMVIAYGIVKKPGTATLTALVQALLVFFTGIIGSHGIMSLITYTCPG
                     IVMDIVLLAIGHRVCCRGCAVIAGAAANVTGTITVNVVFFQVPGVYLILVLSLALLSG
                     AAGGFLAWELIKVIEKYHLVPKTARRKAKDKKTETGTVGPEMSGKKPFKLKLNKWLGF
                     AVIVALFAAAGTAAYMNAESTDKTAKGYSLCISEGDETKGNRTIRTYTLNEIKKMPAE
                     SFKAEIKSSSGEDEEGTYKGVELKYILDSADKKLLENHDRFILSAGDGFSSAVTAEEV
                     KKAGTVFVVYEKDGKELLHLNEGGKGPMRIVMPTDTYGTRSTQFLVKIIGI"
     gene            8932..9615
                     /locus_tag="PLM92_01150"
     CDS             8932..9615
                     /locus_tag="PLM92_01150"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPR23782.1"
                     /translation="MNIIEAKNLTFAYEENTKRHVIENMSFGLKAGTVTVITGLSGCG
                     KSTLCQILCGIIPDFTGGELTGTVMLEGKDITLMEMHEIAKKVGYVMQDPDRQIIAST
                     VEDELAFAPENLCVEPKKIRAEVDRVMNKLGITGLALKNPGKLSGGEKQITAIASVLT
                     LDPDIVIMDEPFSFLDEKGRRMVIEVIEELKREGKTVLVVLHDTAVLPFADKRIHMGE
                     EVIDEPYGK"
     gene            9596..10282
                     /locus_tag="PLM92_01155"
     CDS             9596..10282
                     /locus_tag="PLM92_01155"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPR23783.1"
                     /translation="MSLTVSNVTFSYKKTTKPVLKDFSAEFHSGRITALTGENGCGKT
                     TLAKVIMGILKPYEGRIMLAEEGESDRANGPKETDLSGLSLAETGRKIGYVMQNPARQ
                     IFFTSVSEEIRYGLKNMGFTEDEINFRATEFLRYFDIEKYADTFPFFLSQGEKQRLVL
                     ASVMVMEPAWLILDEPTASLDSKRKILLGDYLTKIRKDKNCGIIVITHDREFAKKYAD
                     SEIRMGGSDV"
     gene            10275..12260
                     /locus_tag="PLM92_01160"
     CDS             10275..12260
                     /locus_tag="PLM92_01160"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbiQ family ECF transporter T component"
                     /protein_id="HPR23784.1"
                     /translation="MFELSRLDPRTKIVMVISISTAAMIIKDILLLTGILLFAIITLA
                     VGGVELKKQMHQAKTVLGMIFFLFILQSLFAWIGGSLYEGALLAGMLSLRLLIIVLSA
                     LIIMTGESRDYLLGMVQWKMPYEIAYMVLIGLHFFPILKEEALDVYYSIQLRGTEMKK
                     TSLRRKLAVYLKISLPVLAGAMARAKAVSISMEARCFRIKPKRTYMRKLKLKTRDILI
                     MVIFPIAAAIIIMTATGGLFAGHLSEGPSVADQKGGDNYEIVLSWTGEPSSTQTVTWH
                     GEKQYEGVVQWTKGETLTKDADAEEAFFEGNSHKKKAEITEVRENEYFSYSVEIAGLK
                     ADTKYSYRVGDGKSWSRTETFTTAPKEEQNKEKGFSFIYMGDIQYELMNRDYKKWDKF
                     ADNAYSQNTDAAFALFGGDLVVNNARTEEYESVMKYGRPMFSNMSIMITPGNHETSIG
                     PYTYKKIFSFPENGPSREGETDPGQIKSTDLAEEVYSFDYGDCHIVSLNSNIFLEERK
                     NDMGEEEWQKMTGDVEDWLTGDLSESHKEWKIAFMHHPAWPVSEDENDYSDTKVYKMI
                     RERWVPIFEKYGIDLVLCGHQHIYMRTEDINGITYIMGRSGEKYSRYYKKGDPLPSYI
                     KKLNEEKTYQVVSVKENRMTVESFGPGGKRVDKWTKN"
     gene            12245..>13499
                     /locus_tag="PLM92_01165"
     CDS             12245..>13499
                     /locus_tag="PLM92_01165"
                     /inference="COORDINATES: protein motif:HMM:NF016032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HPR23785.1"
                     /translation="MDQKLIVRSMKWYNHYMTNQKEKNRKTIAEFFETGSKKDSGCDR
                     IGLELEHFIVDKDTGKSVFYYGEKGIESILKALSPCYEEEIISDGHLIGLKKSDCTVT
                     LEPGAQLELSIAPSKSLKNIMDIQRGFIEDLSVLTDPLGYNIVCLGYREKDKADDIPL
                     IPKNRYRNMDTHFKKTGTMGRNMMRGTGASQVSIDYRDEKDFKRKFRAACFISPLFAL
                     VADNAPFFEGRIYKKNTLRQRIWQNVDDARAGIPWVIDEPDFGFLRYAEYISGIPLIA
                     PGDYDDSYTTKTVDEIYSGRPIKENETAHAASMVFPDVRLVKYIEIRVGDSMPIDLSI
                     SYASMIKGLFLNEKWLDIQEDRFRTFSAADIKRAKKEIEEKLKATMAKLQAATSGKSS
                     RQKLRRAKRQGIAEAEEAKAELRSAD"
BASE COUNT         4634 a         2382 c         3391 g         3092 t
ORIGIN      
        1 taaaaaagta tggagtgagt ggtcaaaggt aaaaactata aaactgtaag aacagaataa
       61 ataaaaaaaa tttcacttct cattatctaa aggtaatgag aagttttttt gtgttgatat
      121 tatatcatca aagctaaaaa atgttgttac aacaacattt tttatgttgt caaaaagata
      181 tactgaatgt taacaaaaag acaattaacg gagaaataaa acaatgagat catccaaaaa
      241 aacatatttg aacagaaaag tgccggctct gataattaca ttatccatga ttgccggtct
      301 tttgggaaac atgggatatg ccaaagcaga agaaactgta tgggatggga cttcggatgt
      361 atcctggtat acggtcgata aggatgcctt gtcatatgaa atagcaacgg ccgaggactt
      421 ggccggcatt gctgaaatag taaacggtac agccgaagac gaatatggcc aaatagaaaa
      481 aaacaatttt gagggagtca cttttacatt gactgctgat attgttctta atactgatcc
      541 cattccaggc agggatgata cccttaagga atggacaccc ataggaggcg gagcaaataa
      601 agaagtattt cgggggacat ttgatgggca gcaatattct attaaaaaca tatatataaa
      661 tgccgacgga tcctggggag aagatgaaaa cagaaatata ggcttgttcg ggtatatagg
      721 cggggaagct gcagttagag atctgacaat aaaagacggc tggatattcg gaagaagagc
      781 agtaggtgca gtggttggca gaacagaaga ttataatgtt tctctggggc acggagccat
      841 aatagagaat tgccataatg aaggcacgga agtgacatca acagataaaa caggagttgg
      901 cggtatcgtg ggagccggtt acaatatggc actaataagc aattgcacca acagcggaga
      961 cataaaagca gatggacaat atgatgccgg cggtatagtg ggagactgcg aaggaataat
     1021 acaaaactgc agtaattcag gcaaagtttc cagcataaca ggaaatgcgg ggggcatagt
     1081 aggctacaat cagcatttcg atacatacgt acctcagacg ataaattgtt ataatacagg
     1141 agaggtttca agcaacggct atgccggagg cattatagga tatcaggaag gcggttcccg
     1201 gaattgttat aatgtgggtg ccctttcggg agatggcaat aagggaggag ttatcggaga
     1261 ccttaacagt cgttatgata atgaaaatct ttattttttg aatagtgagg atagagatat
     1321 tcccggagta gcccggcagc aggcgggaac tacaggaaat gtagaaacaa cagaaaaaac
     1381 tgaagatgaa atgaaaggcg aaccttttcc cggcttattg aacggggacg atacagatta
     1441 tggggaaggg gacgctttca gatatgaggc cggtgattat ccggtatttc cggcatataa
     1501 tgacggcagg gaaaaagtgg tttctgatat caaaataaca gttcctccat ctaccgttta
     1561 ttataaaaca ggagacgtat tgagcctttc cggcatgaaa gttatgataa gttatgccga
     1621 tggcagtgcg gcaacagaga tcacatacga aaacaatacg cctgcaggta ttcttacaac
     1681 tctttcagac agtgatacta gcgtggccgg cacaaaaaca gccgacataa ggtatggcta
     1741 ccgcacagcc aatcaagccc ttatggtgtg tgatgtcaat atcgtctatg gcctgatagc
     1801 tgatcttggg gaaatcaagt gggacagcgg aggaaaactg gttgaagcaa ggtcggcata
     1861 cgctgtactg gaaggcgatg aaggcacaaa caaaggagtt gaaaattaca acgccctgct
     1921 aactgctgaa aactcatata aaaacacgga aggccaatac ggtatagcat atatcggttt
     1981 tggcgagtat atcgataaca taagagacga atataaggaa tccggcatag cagaacttaa
     2041 tgatataaaa agcgagactt taggtataat cgggagccag gaaattgaag ttgcaggaat
     2101 atgggaaaaa ctcaaagaag cggaatccgg aatcactgct ctttcaaaat atctgaaacc
     2161 gagtctttcc tttgcacaga tcgcagttga aacacagaca tatacgggat cggcattaat
     2221 gcctgaaata gtagtgacat acagcggtag aacacttgtg agaaacacgg attatgaggt
     2281 ggcatattca aataacataa aagccagggc atcgggtaaa gctactgtta caggaacagg
     2341 agattacagg gggaccactt cagcaacctt ttatatcgtt caggccgcaa ataaaatata
     2401 ctgcaaggac aggatagata agacatacgg agcaacagct ttcagcctgg gagccaccgc
     2461 ccgtgagggt gccttaatta gttatgcttc gggcaatacc tcggtggcag ctgtttcggc
     2521 cggcggtaaa attacaataa agggaacggg gatcgcaagt ataactgtaa ctgcaaaatc
     2581 aacggccaat tacaaaacaa catcaaaaac aatcacgata tacgttaaac caaaaaaggc
     2641 acgtatttac aaagcgactt caccgaaaaa aagaactttg aaagcaacct ggaaaagaga
     2701 taaaaaagca acagggtatc aggtaatatg tgccaccaac aagagattta caaaaaacaa
     2761 aaaaaccgct ttggtaaaaa agagcaaaac gacatccaag actataaaga agctaaaaaa
     2821 agggaaaaag tattatgtga aagtcagagc atataaaacg gcccggggac agaagatata
     2881 tggttcatac agcaaagtca agacagtaag agtcagatag aagtaggcgg catcattgag
     2941 gcaggaccgg accagaaaat aaaaagacaa aagtaatgaa agaaataatg tataaaaaca
     3001 aaaagataat actgataata gcagcaggtt tggtgctgct gttttcagtt atgtcttttt
     3061 cagttacgac agccgatgct tcagcttcga aacttgatat atatgtggga tattacgggg
     3121 ggccctatta tttgaagaag tcctacagtg tcaatgacat gtattctctt cctactcaca
     3181 gaagggtata ttccttcatt gatgcagcgc caagaccctg tttggactgt gccataggag
     3241 tagatatgga agatcttata agtgatgcga aaatagacat gaattccatt gagactttct
     3301 atttttatac cagcgacagc ggagacacct attatacatc attcccaaaa agtgaattga
     3361 tagatactgt aagatattat tatcctgaac tttacagaca ttttgacaaa ctgcagggag
     3421 gaaccgaagg agcagaagaa ggtgcagtag ccgttccggc tatgctggcc atatcggatg
     3481 attttataaa aatggaggcg ggagacatat ttgttgagaa ttacgatgat cagagttcag
     3541 aaaaccgttt caggcttctg ttcgggcaga ctgataccgt cacaagaaat gcaacaaggt
     3601 cagccaaata tgttggaagg ataaacgtca tgctgggcgg atatcccacc ataacagccc
     3661 ccaattcata cctggacatg aaggtgggaa gcaaaaaaaa catcagtatt acaatagatg
     3721 cggcagatat ccttcttggg aaaaagataa aagaaagcat aacatggaca tcaagtgatg
     3781 aaagtgtggc cactgtggac agaaacggta tggtgacagt cacaggtgaa ggggaagcag
     3841 taataacggc caaatacaaa gatcagtcag tggaagtgac aataaacggg gagaaaggca
     3901 gtgcagccgg ttacggaggg ggaaatggtg gaggcattgg cagtataagt gaagtgagcc
     3961 tgggagaagg agacggaata gatgtcgaaa cctcgggtgg cagcgggcag gcctggcgtg
     4021 tatacgaaat ggatgaaaag gcagaagaac tgccggaaat aaagaagaac aatgatctga
     4081 taccggtcat aataatgata atgatcctgc tgatttcagg aatagcggga agaacattga
     4141 agttttacag agaagtgcga ggttaaaaat gatttcacaa ggactgagtg atgcgctcag
     4201 agctgttgct gaagcagcgc ttacaccggt ggtaataata ctgctggttt tgatagcagt
     4261 gaccataata ctgctgggga ctcttatagc tgaatttttt actgaaaggc gtcatctgaa
     4321 ggtcaaaatg cctgttctga taaaccagat cagagcagct gaaaaaagcc cagaggagat
     4381 aatcgcggga agtggtctga taaaaaaaca aaaagagacc cttaaggaac tggtgcttca
     4441 gagtgaactc accaatacca tgagagaagc gcttgcagta agacttttgg cggagacaag
     4501 ggcccgatat gacggaatag taaatataac caatgtgata gcaaagatag gtcccatgct
     4561 gggacttttg ggaacattga tacctctggg gccggggata atcgccctgg gaaggggcga
     4621 tacttatacc ctttccacat cactgcttac tgcgttcgat actacagtgg caggtcttat
     4681 atgtgccgcg atagcattga tagtttcagt cataagaaaa aagtggtatg ctgactacat
     4741 gtctatgctg gaaacgctta tggaatgtat actggacaag gagaagaaag atgataaggc
     4801 gctttggaac aggtaaatta aaaggcgaag gcattgcaaa agaagatatc aatcctatgg
     4861 aaggcatagc caatctggcg gatgtcatgc ttatactggc ggtcggcata atgctggcgc
     4921 tggtaatgca ctggaacgtt gacataagca caaaagaggt gacccagaag caggagatca
     4981 aagatatgga agatcaggaa ataagcgaaa agatcgaatc acaaaaaggt tttgaggaac
     5041 tgggaactgt ctataaggat cctgaaacag gcaaaatgta tatggtagta agggaggaaa
     5101 aatgaacagg aagaagctta taataatcat agcccttgtt gcggtggttt tggccggtgt
     5161 ggggttttgg gccttttcat cggggctgga taaaggcgca gcccacacaa attactctgc
     5221 agaagagaac tacgggaaag gcaactatgt tttcagtaca gtcaccttta caggaagcgg
     5281 aatggaaagg gaaacagtcc tttcggtaaa ggaaatagag cagctggcat atgataatga
     5341 taaggatctg ggctatgaag agaaatacag ccttctgacc agcggttcca ttttttccga
     5401 gagcactttt acagggataa agctttatga aacactgctt tatctgggga tggataaaag
     5461 ctcaagtgat gatacaagaa taacagttgt ttcctcggac ggatacacca tggtcatgac
     5521 ccttggagaa ataagaagca gcaagtattg tcgttttggc agcgcctcaa atcccaaagt
     5581 tgaggaggag ggactgccgg tacttctctc tttcggttcc ggcggactgc ctctcgtggg
     5641 accgacgggc agtgaagata caggaaaagt gatgacagaa aaagacggat ttgaagagga
     5701 agcccagaac acaggcggcc cggtcaagtt gaccatagga caaaagacct gtgatgattt
     5761 caatgcgcct cagaatgcca aatgggtaaa agaagtgatt gtgggagatc cttacgacca
     5821 cacatttcat gcgggagaat ccggaaaaga aaaagctctt aatgtaagcg tatttgatgc
     5881 agattcggga aacaagaaaa taagcagtaa agaatacaca tttgatgaaa tagaaaaatt
     5941 tgcatcccgg aaaagcgaaa acacgcaaaa gaattactat ggcgaaaagg atttttatga
     6001 aggagccggc atatgggaat tcctgagaga agaaacagaa gtggcagcca gagaaggcac
     6061 agtgaagttc aacttttcag acggcaaaag cgagagagta agtctttcat atttaagaga
     6121 tcttaacggg gatcatgaaa actatatcac tgagaaagaa ggactttata ttacatgcgt
     6181 caaacctgct ttcggataca gtaaaaatgg cgggcccact gagctgggag aactttttgc
     6241 tcttcttccc aaagacggaa aagaaaagaa aaaaagcaca gtaaagaagt gcttatcagt
     6301 tgacgtttat gtgggagatg attatttcgg aagtaaaaac ccctattcca ataaggggat
     6361 cgaaattgaa ggaagcggca tggaagaaga tgtctatatt tatattgaag aaatagagaa
     6421 acaaatagat ctggctgcat catcgggaga ttataaaggg gtcagcctta taggactgct
     6481 ggaagaaaag ggcctggctg ttgatgcaca aaaagctgta atagaaagca gcagcggcag
     6541 cacggaaata agcatcagta cacttaagaa agaagaaaga aatactatac tggccacgag
     6601 aaaaagaggc aaagcccttg atgaagaaga aggacctttg gctcttcgag gcagagatga
     6661 actcaataat gtaaaaaaaa taatcgttta tgccaaagac gggaaatgga gccattcata
     6721 taagggatac aaaaaatatc tgggcagaaa agtaaagatt accggatcgg aagtggcaga
     6781 tgacaggacc tatacactaa aagagctgga atcattaggt gatggatata cagttaaaaa
     6841 tgccttcggt gccggaggag gcaccaatgc attttgcggc gtggttctca aaaagctggt
     6901 gaacgacaat cttaaagagg gcataaaaag accttcaagg ataacagttg cgggcaaaga
     6961 cggatatagt attagtattt cagtaaagga tgtgtataat ggtatagaaa gcaagtacca
     7021 gccgggcgaa acaagggatg taatactggc ttacagcatt gacggaatgc ccatggtgcc
     7081 ttctgaaaaa tctgacggat atagtggcgg aaatgctttc gggcctgtca gactggttgt
     7141 agaaaaccag acttcaaaat gggtaaagaa tgtaaacaga ataataatag gagaatgata
     7201 atgaaaaaaa acaaaatcaa aaagatactg gcggcttctc tttgtatcat cattgccctt
     7261 gcattttctg catgtggcgg cagcgaaagc aacgaaagcg ataataacgg cagcgggaag
     7321 gaaaaatcag aagcagccct tgtgatcacc ggatataacg atgataaaac agaattttcc
     7381 ctggaagata tagaagaaat gggaatggaa aaatatattt tcagcggcag ggacaaaaaa
     7441 gcagacaatg gaagacagat acaggaattc acaggggtgc ctcttaaaga ggtacttgaa
     7501 gcggccggat acggtgccac cggagaaata atgaaagtga tctgcagtga cggatacagc
     7561 cgtgagtatg aaatcgatga tctttttggg ctttatgcct atgcggacga agaagataca
     7621 gaaggagtga aagtggatcc catacttgcc atagaagaaa aagacggaga gaaaataata
     7681 agactggtat acggacagga ggattatgat gctcctgaaa cgaaagattt caacatgcag
     7741 ggatgggcat catggataga gaaaatagaa gttggcaatg aatagattac ttcagaaatt
     7801 tacggtatat gaccttgtaa ttatagcaat aatggcagcc attgggatcg ttattaaacc
     7861 cgtggtggtg ccccttgctc atatagtgac aggtcccttg atgataccca gcggagctct
     7921 ttccggaggg ctttatatga tgtggatggt tattgcatac ggcatcgtaa aaaaaccggg
     7981 taccgcgacc cttaccgccc ttgttcaggc actgcttgtt ttttttaccg gaataatcgg
     8041 ctcccacggg ataatgagtc ttataaccta cacgtgcccg ggcatagtga tggatatagt
     8101 cctgcttgcc atcggacaca gagtctgctg caggggatgt gccgtcatag caggagctgc
     8161 tgcaaatgtg accgggacta tcacagtaaa tgttgttttc tttcaggtgc cgggtgtata
     8221 tctgatactt gtcctttctt tggctcttct atcgggggcg gcgggcggat ttctggcctg
     8281 ggaattaata aaagtaatag agaaatatca tcttgtgcct aagaccgcaa ggagaaaagc
     8341 aaaggacaaa aagacagaga cgggaacagt cggtcccgaa atgtccggca agaagccctt
     8401 caaactgaaa ctgaacaaat ggctgggctt tgcagttata gtagccttgt ttgccgcggc
     8461 cggcactgct gcatatatga atgcagaaag caccgataaa acagccaaag gatattccct
     8521 ttgcatctct gagggagatg aaaccaaggg aaacagaacg atcaggacat atactttgaa
     8581 tgaaataaaa aaaatgccgg cagaaagttt caaggcggaa ataaagtcgt cttcgggaga
     8641 ggatgaagaa ggcacatata aaggagttga actgaaatac atattggatt cagctgataa
     8701 aaaactcctt gaaaatcatg accggttcat actcagtgca ggagacggat tttcatcggc
     8761 tgtcactgca gaggaagtca aaaaagccgg aacggttttt gttgtatatg aaaaagacgg
     8821 caaagaactt ttacacttga atgaaggcgg caagggacca atgaggatag taatgcccac
     8881 cgacacatac ggaacaagaa gtactcagtt cttagtaaag ataataggga tatgaacatc
     8941 atagaagcaa aaaatctgac atttgcatat gaggaaaata cgaaaagaca tgttatagaa
     9001 aacatgtctt ttggtctaaa agccggaaca gttactgtta ttaccggtct ttcaggatgc
     9061 ggcaaaagca ctctttgtca gatcctctgc ggaataatac ctgattttac aggtggggag
     9121 ctgaccggca ctgttatgct ggaaggaaaa gatataactc ttatggaaat gcatgagatt
     9181 gcaaaaaaag tcggatatgt tatgcaggat cccgacaggc agatcattgc ttcgactgtg
     9241 gaagacgagc tggcttttgc tcccgagaat ctctgtgtcg aaccaaagaa gatacgagcc
     9301 gaagtggaca gagtaatgaa taaactgggg ataacaggac tggccttaaa aaatccgggc
     9361 aaactttccg gcggggaaaa acagataaca gcaattgctt cagtgctcac ccttgatccc
     9421 gacatcgtta tcatggacga accttttagt tttcttgatg aaaaaggacg caggatggtg
     9481 attgaagtaa tagaagaact gaaaagagaa ggtaaaacgg tacttgttgt tttgcatgac
     9541 actgctgtac ttccctttgc agacaaaagg atccatatgg gagaggaggt cattgatgag
     9601 ccttacggta agtaatgtga ccttcagtta caaaaaaaca acaaagcccg tactaaaaga
     9661 tttttcagca gagtttcatt cgggaagaat aaccgctctg acaggagaaa acggatgtgg
     9721 caaaaccacc ctggcaaagg taataatggg catcctgaag ccatacgagg gcaggataat
     9781 gctggccgaa gagggggaaa gcgacagggc aaacggaccg aaagaaacag atctttcggg
     9841 tctttccctg gccgaaacag gcagaaaaat aggatatgtt atgcagaacc cggccaggca
     9901 gatatttttc accagcgtca gtgaagaaat acgatacggt ctcaaaaaca tggggtttac
     9961 ggaggatgaa ataaatttca gggcaacaga gtttttaaga tattttgata tagagaaata
    10021 tgcggacaca ttcccttttt ttctgagcca gggagaaaag caaaggctgg tactggcatc
    10081 ggtaatggta atggagcccg catggctgat acttgatgaa cctacagcat cccttgacag
    10141 caaaagaaaa atactgctgg gagactatct gacaaaaatc agaaaagata aaaactgcgg
    10201 gataatagtg ataacccatg acagagagtt tgcaaaaaaa tatgcagaca gcgagataag
    10261 gatgggtggc agcgatgttt gaattatccc ggctggatcc caggaccaag atagtgatgg
    10321 tcatttccat atcgacggcg gcaatgataa taaaagatat tctgctcctg accggcattc
    10381 tgcttttcgc cataattacc cttgccgtcg gaggagtaga gctaaaaaag cagatgcacc
    10441 aggcaaaaac tgttctggga atgatttttt ttctgttcat actgcagtcc ctttttgcct
    10501 ggataggagg ctctctttac gaaggagccc tgctggcagg gatgctttca ctgcggcttt
    10561 tgataatagt gctttctgca ctgataataa tgacaggaga gtcaagggat tatctgcttg
    10621 gaatggtcca gtggaaaatg ccctatgaaa tagcatatat ggttcttata ggtcttcatt
    10681 ttttccccat actgaaagaa gaagcactgg atgtgtatta cagcatacag ttgagaggga
    10741 cagaaatgaa aaagacctcg cttcgaagaa aacttgcagt ttatctcaaa ataagtcttc
    10801 cggtccttgc gggagccatg gccagagcaa aggccgtttc catatccatg gaagcacgat
    10861 gtttcaggat aaaaccaaaa agaacatata tgagaaagct aaaactgaaa acacgtgata
    10921 ttctgatcat ggtcattttt cccattgccg cagcaattat tataatgacg gccaccggag
    10981 gcctttttgc aggacattta tccgaaggac cctctgttgc agatcaaaaa gggggagaca
    11041 actatgagat agttctttcc tggaccggtg aaccttcgtc cacccagacc gtgacctggc
    11101 atggggaaaa gcagtacgaa ggggtcgtcc agtggacaaa aggagaaact cttacaaagg
    11161 atgcggatgc agaagaagct ttctttgagg ggaactctca taaaaaaaag gctgaaataa
    11221 ctgaagtaag agaaaatgaa tacttttcat acagcgtgga aatagcaggc ttgaaggcgg
    11281 atacaaagta cagttaccgg gtcggtgacg gaaagagctg gagcagaacg gaaaccttca
    11341 ctacggcacc caaagaagag cagaacaaag aaaaaggatt ttccttcata tatatgggag
    11401 acatacagta tgaacttatg aatagagatt ataaaaaatg ggataaattc gcagacaatg
    11461 cgtattcgca gaatactgat gctgcctttg ctctttttgg cggggatctg gtggtgaata
    11521 atgccaggac agaggaatat gaatcggtga tgaaatacgg caggcccatg ttttccaata
    11581 tgagtataat gataacgccg ggcaatcatg aaacctccat aggtccgtat acatataaga
    11641 agattttttc ttttcccgaa aacggccctt cccgtgaggg agaaacagat ccggggcaaa
    11701 taaaaagtac tgaccttgcc gaagaagtat attcattcga ttacggcgac tgccatattg
    11761 tttctctgaa cagtaatata tttcttgaag aaagaaaaaa tgatatggga gaggaggagt
    11821 ggcagaagat gaccggggat gtagaagact ggctgaccgg tgatctttca gaaagtcata
    11881 aggaatggaa gatagctttc atgcaccacc ctgcatggcc ggtcagtgaa gatgagaatg
    11941 actacagcga tacgaaggtt tacaaaatga taagggaacg ctgggtgcca atttttgaga
    12001 aatacggtat agatctggtc ctttgcggtc atcagcatat ttatatgcgc acagaggata
    12061 taaacgggat cacatacata atggggcgtt cgggagaaaa atattcccgc tattacaaaa
    12121 aaggcgatcc cttgccttca tatataaaaa agctgaatga agaaaagact tatcaggtag
    12181 tttccgtgaa ggaaaacaga atgacggtag agtccttcgg gcccggaggc aaaagggttg
    12241 ataaatggac caaaaactga ttgtacggtc catgaagtgg tataatcatt atatgacgaa
    12301 tcaaaaagaa aaaaacagaa agacaatcgc agaatttttt gagacgggtt caaaaaaaga
    12361 ctcgggctgt gacaggatag gacttgagct ggaacacttt atagttgata aggatacagg
    12421 caagagtgtt ttttattatg gagaaaaggg aatcgaaagc attctaaagg ccttgtctcc
    12481 ttgttatgaa gaggaaataa tttccgacgg gcatctcatc ggacttaaaa aaagtgactg
    12541 caccgttacg ctggaaccgg gggctcagct ggaactttcc attgccccgt ccaaaagcct
    12601 gaaaaatata atggacatac aaagaggatt catagaagac ctttctgttt tgaccgatcc
    12661 tctgggctat aacatagtct gtctcggtta cagggaaaaa gataaagcag atgatatccc
    12721 tttgatacct aaaaacagat acaggaatat ggatacccat ttcaaaaaaa ccgggaccat
    12781 gggcaggaac atgatgaggg gtaccggggc ctcccaggtt tcaatagatt atagagacga
    12841 aaaagacttc aaaagaaaat tcagggcagc ctgtttcata agtcctcttt ttgcccttgt
    12901 tgcagacaat gctccctttt ttgagggaag gatatataaa aaaaatacac tcagacagcg
    12961 catatggcag aatgtggatg atgccagagc aggcatacca tgggtaatag acgaaccgga
    13021 tttcgggttt ctcaggtatg ccgaatatat atccgggatt cctttgatag cacccggtga
    13081 ttatgatgac tcatatacca caaaaacagt tgatgaaata tattctggaa gaccaatcaa
    13141 agaaaacgag acagcccatg ccgcatccat ggttttcccg gatgtgaggc ttgttaaata
    13201 tatagaaata agagtcggcg acagcatgcc catagacctg agtatttcct atgcatccat
    13261 gataaaaggt cttttcctga atgaaaaatg gctggatata caggaagata gattcaggac
    13321 cttttctgcg gctgatataa aaagggcgaa aaaagaaata gaagagaagt tgaaggctac
    13381 tatggcaaaa cttcaagctg ctacttctgg aaaaagtagt cgtcagaaat tacgtcgtgc
    13441 aaaaagacaa ggaattgcag aagcagaaga ggctaaagca gaattaagaa gcgcagatg
//
LOCUS       DAONHK010000015        13418 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_3611_length_13418_cov_4.109332, whole genome shotgun sequence.
ACCESSION   DAONHK010000015 DAONHK010000000
VERSION     DAONHK010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 13418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13418
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3611_length_13418_cov_4.109332"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..634)
                     /locus_tag="PLM92_01170"
     CDS             complement(<1..634)
                     /locus_tag="PLM92_01170"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPR23786.1"
                     /translation="MKKGYGGFITLYTFSYMAIGVMMPLIGQYLKHAGFSGTQIGIIT
                     ASGTFAAILGSTFWGKVYANAVSKKILLVFLCFFAAVTSVALSGIYIYVVFILGYAVM
                     YFFQAPVMALSDALTIDEKKPFGYVRMWGAVGFALGIFVSANIAEALGLGSIFFIYAG
                     CFIICAWAVIMICRREKSGRECSAPVGKTKKVQRYRYRDLRNNKKLLMLLL"
     regulatory      707..799
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            874..1401
                     /locus_tag="PLM92_01175"
     CDS             874..1401
                     /locus_tag="PLM92_01175"
                     /inference="COORDINATES: protein motif:HMM:TIGR04518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HPR23787.1"
                     /translation="MKTKNNTYVIVIVALMIALEVILNRFVGITAEAIHVGIDFLPIA
                     VVAMLYGPLWAGAAYAIGDLIGALLFPYGAINPGITITLALVGITYGIVFYRKDLSGR
                     KIIWRSLVASLITAVPIKLFGTTLALALFYGTPYTVLLVTRIPNCAILFAAQMILIPI
                     IYKLVVSKLTIYKTA"
     gene            complement(1482..1724)
                     /locus_tag="PLM92_01180"
     CDS             complement(1482..1724)
                     /locus_tag="PLM92_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HPR23788.1"
                     /translation="MRTDIFLKNSRLIKRRTVAKEACEKGRIDVNGRQAKPGTELKVG
                     DMITISFGEKKIKAVVKELNASPKKEESAEMYEIVE"
     gene            complement(1780..2058)
                     /locus_tag="PLM92_01185"
     CDS             complement(1780..2058)
                     /locus_tag="PLM92_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HPR23789.1"
                     /translation="MNKAELIAAVAEKTGFTKKDAEVVVNATMSTIEGALVKGEKVQL
                     IGFGTFETRSRKARTGRNPRKPGETIQIAASKAPVFKAGKALKDSVNK"
     gene            complement(2079..2762)
                     /gene="mazG"
                     /locus_tag="PLM92_01190"
                     /pseudo
     CDS             complement(2079..2762)
                     /gene="mazG"
                     /locus_tag="PLM92_01190"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
     gene            complement(2778..2933)
                     /locus_tag="PLM92_01195"
     CDS             complement(2778..2933)
                     /locus_tag="PLM92_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPR23790.1"
                     /translation="MKNEKYADLMDEAKNSEEGLSRLYEILKVLRKECPWDRKQTHES
                     IRTCMIE"
     gene            complement(2941..4575)
                     /locus_tag="PLM92_01200"
     CDS             complement(2941..4575)
                     /locus_tag="PLM92_01200"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HPR23791.1"
                     /translation="MSGKTLIKGAATLGAAGLIVKLLGAAFRIPLVNWIGDTGMANYN
                     PAYYIYNFFIILATAGIPVAISKMVSERMAVGDFADAHRVFKLSMMLMTAIGTVSFLI
                     LFIFADPIAVLCNNPQAALAMRAISPCLIFVPLQAAYRGYFQGMQNMRPTAVSQVTEQ
                     FFRVAIGLFLAYTLFNMAGGGDFLMSFDKYERGAAGATFGATAGSVGGLLIILIIYGL
                     SRKAIKYRIRRSQNEDGERNGEIFKRILMIAVPITIGASIMPLMNFLDVPIVIGRLQH
                     AGYGYEEAKSLYGQLGGFAGSLVYFPQVLIQAVAMSLVPLVSSYYRTGDLDLMRSNVS
                     LGLRMSCIIGFPCALGLMSLSEPIMLLLYPAQTESAISAADLLFTLSIGIVFLSVSQT
                     LTGVLQGIGKQMIPVRNLAIGVAVKIAITWILTGIPTFNVQGAAIGTVCAFAIASILN
                     YTAARKYTGAFINKKNTFGKPLLASLIMYGAVVGIYKLLGYFISGNTLPTLIAITAGV
                     VVYAAMIFATRAVTKEEISRLPKGAKLVRVFGRFLR"
     gene            complement(4590..5876)
                     /locus_tag="PLM92_01205"
     CDS             complement(4590..5876)
                     /locus_tag="PLM92_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016147659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HPR23792.1"
                     /translation="MLFKDISILDRDFEIREHCFVGTEGERISFIGNKMPQKDFGEVF
                     PGKGRLLMPGFYNSHAHSPMTLLRGYGENMALQDWLNKKIFPFEAKLKGEDIYWGTML
                     AMAESIRYGIVSTSDMYYFTDEMARAVDESGAKNNMSRAITNFGDADIWDIEGAMEMK
                     KAYEGLNGTAGGKILIDMSLHAEYTSTPKTVRQLAEYTKEIGANMHVHVSETRTEHES
                     CKEKYKMTPLAYLNSHGLFDTPTTAAHCVFIEGDDYSILSEKGITVACNMTSNMKLAS
                     GICDAGKLLKRGINVTVGTDSVASNNSLNMIEEIKFFALAPKVKTGDPSVITPKEALR
                     AATINGAKAQGRKDCGILETGYRADMIVLDITGPSMHPSHDMKNNIVYSASGSDVIMT
                     MVDGKVLYKDGEYTTIDIEKTIFEAERATKEILAGL"
     gene            complement(5920..9144)
                     /gene="mfd"
                     /locus_tag="PLM92_01210"
     CDS             complement(5920..9144)
                     /gene="mfd"
                     /locus_tag="PLM92_01210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HPR23793.1"
                     /translation="MGIVSITGISDSRVAAVCADIIKKKGGQFLIVAPTFVRAGQLAS
                     DLSFFTDEKICVMPSEEETFLNYEAKNNDSLIERLNILKTLAEGDRCIVVAPVSRVIK
                     KLPPHSTFLESVSVLRTGETMDVHLLEERLTETGYERVPMVYAKGQYSKRGGIVDIFA
                     PYNELPYRIDFFDDEIESIRLFDPESQRSLKKIHMVSLYPARQIVGGPSIYDDALRKI
                     EKNYADMDERREQLKEAVITRTNMQHLENYIDYFYKSPEYIWDYMHGGTVIIDDPSKA
                     MEKAEAKAKGFRHDFDVFLEKGVVSKADRKYLPGVSDYLKLYEREKVFFMMPFAKVVK
                     GVDTYEETHNIKSRQSMSFNGKMDILERELKMLIEDGYKVTLVLSTNERMANMQDFID
                     RCGLGERVWLKNGMLSEGMVFPEEKKCMITDGDIFGIRKYSSKRRSRYRGGEPIKSFS
                     DIGKGDYVVHDSHGIGRFIGIQQLNVRGIKKDYFKIEYAGKDMLYVPVEQMDIVQKYA
                     GADAKVPRINKLAGKEWQNVKAGAKAAVAGMARELLETSAARKEQGGYAFGRDTVWQK
                     EFEESFPYEETPDQLRCIEEIKADMEKPMAMDRLLCGDVGYGKTEVAARAMFKCVVEG
                     KQAVVLVPTTILASQHYHTLKDRFEKFPFSVEMLSRFRTDAQQKKILHDAENGTVDVI
                     IGTHRLLSRDVRFKNLGLLVIDEEQRFGVRHKEAIKKLRMNVDVLTLSATPIPRTLHM
                     SLMGIRDMSLIEEPPEERYPVQTYVTEQEDYIIKEAIQRELERGGQVYVIYNRVSGIY
                     KTADMIDRLVPGRKISVGHGRMNETALEDVMLEFIGGGSEILVATTIIESGIDIQNVN
                     TVIVLDADKYGLSQLYQLRGRVGRGNRLAYAYLMHKKNKILSETADKRLRAIKDFTEF
                     GAGFKIAMRDLEIRGAGNLLGSKQHGHMINVGYEMYLRLLAEAVKALGGETGMPEKEE
                     ALVELSVEAVIPQTYIEDEMTRLEMYKKIAGVTDKASAEEMRQELMDRFGEIPEETEN
                     LIKVSYIKSLAENLGIKKVTQKGAKTPDEIIETLSLVQNNENMI"
     gene            complement(9135..9719)
                     /gene="pth"
                     /locus_tag="PLM92_01215"
     CDS             complement(9135..9719)
                     /gene="pth"
                     /locus_tag="PLM92_01215"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004223891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HPR23794.1"
                     /translation="MFVVVGLGNPGRKYAKTRHNMGFIVADEFAEKLDIKINKIKHKS
                     LVGEGYLAGQKVLLAKPQTYMNLSGEAVREIMHYYKVEPEKMILIYDDIDIETGRIRI
                     RKKGGPGTHNGMKSVVAAMGTEEFPRIRIGIGGDIKMHLMDFVTGGMNKKEKEILKGA
                     VITAAEATECIIEKGIDAAMNRYNSRVSEKEEWE"
     gene            complement(9734..10684)
                     /locus_tag="PLM92_01220"
     CDS             complement(9734..10684)
                     /locus_tag="PLM92_01220"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006001952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HPR23795.1"
                     /translation="MKNAGFSDFKVFAGNSHLELADEITSIMGKPLGKANVTKFSDGE
                     ISVNLWETVRGVDCYIIQSTCYPVNDNLMELIIMMDAMKRASAGRINAVVPYYGYARQ
                     DRKAKARDPITAKVVADLLVTAGADRVITMDLHAAQIQGYFNIPVDHLIGLPILVKYF
                     TKKNIKDLVIVSPDHGSVTRARSMAEPLDAPIAIVDKRRPEPNKSEIMNIIGDIKDKN
                     CVLVDDMIDTAGTICNAGKALMEMGAKSVSACATHPVLSGPAIERLDASDFDEVVLLN
                     TIPMAEEKKHKKFKMLSVAPLFAESMMRIHTNDSISKLFD"
     gene            complement(10704..11399)
                     /locus_tag="PLM92_01225"
     CDS             complement(10704..11399)
                     /locus_tag="PLM92_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006001950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HPR23796.1"
                     /translation="MNFEEYEKQEKERIRKNLFFAEKGVRFIDIKQVYIEGNKVDIKK
                     GTVLYPGVILEGSVTIGEDCVIGPGTRITESEIGDRTQIQNSVITESRIGTETKIGPF
                     AYLRPGSDVGSGCRIGDFVEIKNSVFGDGSKASHLSYIGDSDIGKDVNIGCGVVFVNY
                     DGSKKYRSKVGDGTFIGCNVNLVSPVTLEEGSYIAAGSTVTKDVPGGALYVERSKGRV
                     LEGWVEKRGILKK"
     gene            complement(11396..12805)
                     /gene="murC"
                     /locus_tag="PLM92_01230"
     CDS             complement(11396..12805)
                     /gene="murC"
                     /locus_tag="PLM92_01230"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005838580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HPR23797.1"
                     /translation="MIDLANVKQVHCIGIGGVGLSAIAGILLSEGYLVTGSDMNQNDK
                     TEQLIKAGANIFLGHRAKNVEGADIIVYSAAVTKDNPEMVRGKEMGIPCVSRAQMLGH
                     LMKGCKNSIAVAGTHGKTTTTSMISLILEKAKKDPTILVGGNLSEIGGNVKVGKSQFF
                     ITEACEYMDSFLELKPRIEIILNIDSDHLDYFKDIEHIASSFEKFAALVPEDGMIFAY
                     DANPFVNRIIQDLPNVITFGFNDKCDYYAKEIEFNADGMPAFEISSKGKKLCKLQLSI
                     PGEHNILNALVATACCSVLGVDINDIKETLEKYKGTQRRFDVLGETQSGIRIVDDYAH
                     HPAEIRATIEAAINTPHKNIWCIFQPHTYTRTLALFDEFPASFEKADKVVLAEIYAAR
                     EKNVYQISSKDLMAEIKKSYPEKEVYYFGSLEEIASFVYNNAQPGDLIITMGAGDVYR
                     AGEMILEKDAVTGRGEKIS"
     gene            12937..>13418
                     /locus_tag="PLM92_01235"
     CDS             12937..>13418
                     /locus_tag="PLM92_01235"
                     /inference="COORDINATES: protein motif:HMM:NF020744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HPR23798.1"
                     /translation="MKRTERIGAIIKILSDSPSRPFGLSYFCDLLGAAKSSISEDLKS
                     AREIVSSMELGLIETIPGARGGVIFIPHIYDDQCLALQQELCDLIKDPFRMLGGGFLY
                     TSDIMFNASLTKKMALVFARKFRNSGAEYVATIETKGIPLALMTSWMLNIPAVVVRRE
                     T"
BASE COUNT         3187 a         3316 c         2563 g         4352 t
ORIGIN      
        1 acagcaaaag catcaaaagt tttttattgt tccttaaatc cctgtatctg tatctttgta
       61 cttttttcgt ttttcccaca ggggcagagc attcccgtcc ggacttttct ctcctgcata
      121 tcataatgac cgcccaggca catataataa aacagccggc ataaataaaa aagatgctgc
      181 caagtcccaa agcctcagca atatttgcac ttacaaatat gcccagggcg aaacccactg
      241 ctccccacat ccttacataa ccgaaaggtt ttttttcatc aatggtaagg gcatcggaaa
      301 gagccattac cggagcctgg aaaaaataca tcactgcgta gcccaaaata aaaactacat
      361 atatataaat accggataaa gcgacagatg tgacggcagc aaaaaaacaa agaaacacca
      421 gcagaatttt tttgcttacg gcgttggcat aaactttgcc ccagaatgtg gacccaagta
      481 tcgccgcaaa agtgccggat gcggttatga ttccgatctg tgtaccggag aatcccgcat
      541 gtttaaggta ctgtccgata agaggcatca tgactcctat ggccatatat gaaaatgtgt
      601 atagtgtaat gaatccgccg tatccttttt tcattttacc acctcaaaga aagtatatca
      661 gagtaacctg ttgcatacaa taacggcctg tgctaaaatc atggcatgta gagtaaatac
      721 cgtgcgtcaa gtgtcataag atgggatgtt gcttatgaac gaaaagcatg gcttacgatt
      781 cggtattgca tccactacta cattaaagat gatgaaaagc aactcttttc actttagtgt
      841 agttgcgcta aagtaaagaa aggagttttt ttgatgaaaa caaaaaacaa tacctatgtc
      901 attgttattg ttgcactcat gatcgcactc gaggtgatac ttaaccgttt tgtaggtata
      961 acggcagagg ctatacatgt gggcattgat ttccttccga tagccgttgt ggccatgctt
     1021 tacggtcccc tttgggccgg ggccgcttat gccataggag acctcatagg agcacttctt
     1081 tttccttacg gagccataaa cccggggata acaatcactc ttgctctcgt tggaataacc
     1141 tatggtatag ttttctaccg gaaagatctt tccggtagaa aaataatatg gcgttccctg
     1201 gtggcttctc ttattacagc tgttccaata aaacttttcg gcaccactct ggcccttgct
     1261 ttgttttacg gaactcctta tacggtcctg ctggtgaccc ggataccaaa ttgcgccata
     1321 ttgtttgctg cacagatgat tctgatcccc ataatataca aactggtggt gtcaaaactt
     1381 actatatata agactgcata ataaaaagcc gcacggaatc atatgtgcgg ctttactttt
     1441 ttatttcgca gggctttgct tctatttttg gagccgttct atcattcaac tatttcatac
     1501 atttctgccg actcttcctt ttttggagaa gcattgagct ctttcacaac agcttttatt
     1561 tttttttcac cgaaactgat tgtgatcatg tctcccactt tcagctcagt tccgggtttt
     1621 gcctgtctgc cgttcacatc gatacgtccc ttttcacagg cttcctttgc aacggtcctt
     1681 ctttttatca gtcttgaatt tttcagaaaa atatctgtcc tcatttcact tctcttcctg
     1741 acataaaaaa ggggcaactc cgttgcccct ttcgtttttc tatttgttta ccgagtcttt
     1801 tagtgctttt cctgctttga atacaggtgc ctttgaggct gctatctgta tagtctctcc
     1861 gggtttcctc gggttccttc cggttcttgc ttttctactg cgtgtttcaa aagttccgaa
     1921 accgataagc tgtacttttt ctccctttac aagagctccc tcgatggttg acattgttgc
     1981 gttcacaaca acttctgcgt ctttttttgt gaatcctgtt ttttctgcta ctgcagcaat
     2041 caattcagct ttgttcatta gtagatcctc cttaaaaatt atctttgttt catgcgctta
     2101 tgcgcaaacc cttgcagggt ctcccgagat ttagcttctt cccatatttg atccatttcc
     2161 tgaatactca tttcttcaag cttcatgcct ttttttcggg ctacttcttc tatccgttca
     2221 aatcttttta tgaatttgtc cgttgccgca ttcagtgctt cttcagggtc aatattcaga
     2281 aatcgagccg agttcactac cgaaaacaat aagtctccta tctcaatagc aatttcatcc
     2341 ccgttttcat tctgataagc gataaataat tcctcagttt cttctgaaat tttgttgaaa
     2401 gcccccgcaa tatcatccca gtcaaaaccg tatcgtgccg ctcgctcttg cacctttttg
     2461 cttcttataa gcgccggaag tgatcttggc actccatcaa gtctggctgt aaggtcgtct
     2521 tttatacgct cttcatcttt aatattctcc catttttcaa ggactttgtc aatagtttta
     2581 gcgtttccgt ctgaaaaaac atgagggtgt ctccttatca ttttgtcaca ttcctcgttt
     2641 atcacatctg tcagtgaaaa ctctttttct tcttccgcca gtgatgcatg aaaaaggacc
     2701 tgaagcaaaa catcccccag ttcttctctt agattttctt tgtcttgatt gtttatggct
     2761 tcaacagtct cataagccta ttcgatcata catgtcctta tgctttcatg ggtctgtttt
     2821 ctgtcccagg gacattcttt tcgcaaaact ttcagtattt cataaagtct tgaaagaccc
     2881 tcttcgctgt tttttgcctc atccatcaga tctgcatatt tttcattttt catatcagta
     2941 ttacctcagg aaccttccga ataccctgac aagtttagct cccttgggaa gccttgatat
     3001 ctcttctttt gttacagccc ttgtagcaaa tatcattgcc gcatatacta ctactcccgc
     3061 cgttattgct ataagcgtag gcagtgtgtt cccggagatg aaatacccca gcagtttata
     3121 gatgccgaca acagcaccat acatgataag ggaagccaga agaggctttc cgaaagtgtt
     3181 ctttttattt ataaaagctc cggtgtattt tcttgccgca gtataattca gtatcgatgc
     3241 aatggcaaag gcacatacag tacctatggc ggcaccctgt acattgaagg tgggtatgcc
     3301 cgtaagtatc catgttatag ctatttttac ggcaacgcct attgcaagat ttcttacggg
     3361 tatcatctgc tttcctatcc cctggagcac tcccgtcaag gtctgggaaa cagaaagaaa
     3421 tactatcccg atagaaaggg tgaacaaaag gtctgctgca cttatcgcac tttccgtctg
     3481 agccggataa agcaacagca ttatgggttc tgacagggac atcagcccca gtgcgcaggg
     3541 aaatccgatt atgcaggaca tacgaagtcc cagagaaaca ttgcttctca tcaggtcaag
     3601 atcccccgtc cggtaatagg atgagaccag tggaacaagg ctcatggcaa ccgcctgtat
     3661 caacacctga ggaaaatata ccagagaacc ggcaaaacct cccagctgcc cgtaaaggct
     3721 ttttgcctcc tcgtatccgt atcctgcatg ctgcagtctt ccgatcacaa tgggcacatc
     3781 aaggaaattc ataaggggca ttatagatgc tcctatggta atgggcacag caatcattag
     3841 tatcctctta aatatttcgc cgtttctttc accatcttcg ttttgtgatc ttcttatacg
     3901 gtattttatg gcttttctgc taagaccata tatgatcagt attataagaa gcccgccgac
     3961 ggatccagct gttgctccga aagtcgctcc cgccgctccc ctctcatact tatcgaagct
     4021 cataaggaaa tctcctccgc ctgccatatt aaaaagagta tatgcaagga aaagtcctat
     4081 ggccactctg aagaactgtt cagtgacctg tgaaacggcc gtgggtctca tattctgcat
     4141 gccctggaaa tatcctctgt atgccgcctg cagaggaaca aaaatgagac acggggaaat
     4201 ggctctcatg gccaatgccg cctggggatt gttgcacagc actgctatgg gatcggcaaa
     4261 aataaatagt atcagaaaag ataccgtgcc tatggcagtc ataagcatca tggaaagttt
     4321 aaacaccctg tgagcatcag caaagtcacc taccgccatt ctttcggaaa ccatttttga
     4381 gatggcaaca ggtatccccg ccgtagccag tattataaag aaattgtaaa tataatatgc
     4441 gggattataa ttcgccatgc cggtatcgcc gatccagttc acaagaggta tcctgaatgc
     4501 ggctcccagc agttttacta taagccccgc ggcgcccagt gtagccgctc cttttataag
     4561 ggtttttcct gacatcttac ctccgcatat cagagcccgg ccagtatttc ttttgtggct
     4621 ctttcagctt caaaaatagt tttctctata tcgatggtag tgtattcacc gtctttgtaa
     4681 agcactttgc cgtcaaccat ggtcattata acatcgcttc ccgatgcaga atagactata
     4741 ttgtttttca tgtcatggga tggatgcatt gaagggcctg taatatcaag cactatcata
     4801 tccgccctgt agcctgtttc cagtatcccg cagtcttttc tgccctgggc ctttgccccg
     4861 tttatggtgg ctgctctgag ggcttctttg ggggttatca ctgatggatc tcctgttttc
     4921 accttgggag ccagggcaaa aaacttgatt tcctctatca tgttcaagct attattactg
     4981 gccacactgt cggttcccac agtaacattt atgccccgtt tcagcagttt cccggcatcg
     5041 catatgccgc tggcaagttt catgttgctg gtcatattgc atgcaacagt tattcctttt
     5101 tcggaaagta tgctgtaatc atccccttct atgaatacac agtgagccgc cgtcgtgggc
     5161 gtatcaaaaa gtccgtggct gttcagatat gcaagggggg tcattttgta tttttcttta
     5221 caactctcat gttccgttct ggtttcggaa acatggacat gcatattggc tccgatctct
     5281 tttgtgtact cggccagctg tcttactgtt tttggggtgc tggtatactc ggcatgaaga
     5341 ctcatgtcga tcaggatctt tcctcctgcg gtaccgttta atccttcata ggcctttttc
     5401 atttccatgg ccccttcaat gtcccagata tccgcatcac caaagtttgt gatggctctg
     5461 gacatattgt tttttgcgcc gctctcatca actgcccttg ccatttcgtc agtgaaataa
     5521 tacatgtcgg aagtagatac tattccgtat cttattgatt ccgccatggc aagcattgtt
     5581 ccccaataaa tatcctctcc cttgagcttc gcctcaaagg gaaatatttt tttgttcagc
     5641 cagtcctgaa gtgccatgtt ctctccatat ccccttagaa gagtcatggg tgaatgggca
     5701 tggctgttat agaatcccgg catcagcagc cggccttttc cggggaaaac ttctccgaaa
     5761 tctttttgag gcattttatt tccgatgaaa gatattcttt caccttcggt gcccacaaag
     5821 cagtgttctc ttatttcaaa gtccctgtcc agtattgaaa tgtccttaaa cagcatgaaa
     5881 aatgcctcct tgcagatgta tgaaataaac caacgaatat tatatcatat tttcgttgtt
     5941 ctgtaccaga ctcagtgttt ctatgatttc atcgggagtc ttagctccct tttgcgttac
     6001 ctttttgatg cccagatttt ccgccaggga ttttatatat gatactttta tcaggttttc
     6061 cgtttcttct ggtatttctc cgaatctgtc cataagttcc tgccgcattt cttcagcaga
     6121 tgctttgtcc gttacccctg cgattttttt gtacatttcc agtcttgtca tttcatcttc
     6181 tatgtaggtc tgaggaatta ccgcttcaac agaaagttca actaatgctt cttccttctc
     6241 aggcataccg gtttctccgc caagggcctt tactgcttct gcaagaagtc tcaaatacat
     6301 ctcatatccc acattgatca tatgaccatg ctgctttgaa ccaagcaggt ttcccgcccc
     6361 tcttatctcc agatctctca ttgctatttt gaatccggct ccgaattcgg taaaatcctt
     6421 gatggctctt aatcttttgt ccgccgtctc cgaaagtatc ttgttctttt tgtgcataag
     6481 atatgcatat gccagtctgt tgcctcttcc cactcttcct ctcagctgat aaagctgaga
     6541 gagtccgtat ttgtcggcat caagtacgat cacggtattg acattttgta tatctattcc
     6601 cgattctata atagttgtgg caacaagtat ttcagacccc ccgcctatga attccagcat
     6661 tacatcttca agggctgtct cgttcatgcg gccgtgtccc actgatattt tccttccggg
     6721 aaccagtctg tctatcatgt ctgcagtctt atatatgccg cttacccggt tatagataac
     6781 atatacctgc cctcctcttt ccagttctct ttggatggct tcctttatta tgtagtcctc
     6841 ctgctcagta acataggtct gcacagggta tctttcttcg ggtggctctt cgatcaggct
     6901 catgtccctt atgcccataa gggacatatg aagggtcctt ggtataggcg tggcggaaag
     6961 agtcagaaca tccacattca ttctgagttt ttttatggct tctttgtgcc tgacgccgaa
     7021 gcgctgttct tcatctatca ccagaagtcc cagattcttg aatctgacat ccctggaaag
     7081 caggcggtga gttcctatta tcacatctac tgttccgttt tctgcatcat gcaatatttt
     7141 tttctgctgt gcatctgttc tgaaccttga aagcatttca acggaaaagg gaaatttctc
     7201 gaaacggtct tttaatgtgt gataatgctg acttgccagt attgtcgttg gcaccagcac
     7261 cactgcctgt tttccttcga cgacacattt gaacattgct cttgcagcca cctctgtctt
     7321 gccgtatccc acatcaccgc aaagaagtct gtccatggcc atcggttttt ccatatctgc
     7381 ttttatttct tcgatgcagc gcagctgatc tggcgtttcc tcatagggga aactctcttc
     7441 gaattccttc tgccagacgg tatccctgcc aaaagcataa ccgccctgtt cttttcttgc
     7501 tgcagatgtc tccaacagtt ctcttgccat gcctgcaacg gctgcctttg ctccggcctt
     7561 gacattctgc cattcttttc ctgccagttt gtttatcctg ggaacttttg cgtcggcacc
     7621 ggcatatttc tggactatat ccatctgctc caccggaaca taaagcatat ccttaccggc
     7681 gtattctatt ttgaaataat ctttctttat cccccgtaca ttgagctgct gtatgcctat
     7741 gaatcttcct atcccatggc tgtcatggac cacataatca cccttgccaa tgtcggagaa
     7801 gctttttatc ggttccccac ccctgtacct gctccgtctt tttgaagaat acttcctgat
     7861 gccgaatatg tccccatcgg tgatcataca ttttttttct tcggggaaca ccataccttc
     7921 tgaaagcata ccgtttttca gccataccct ttccccgagg ccgcaacgat caatgaaatc
     7981 ctgcatattg gccatacgct cattggtgct caataccagg gtcactttgt agccgtcttc
     8041 aataagcatt ttcagttctc tttccagaat gtccatcttg ccgttgaaag acatgctctg
     8101 acggcttttt atgttgtgtg tttcttcgta agtgtctaca ccttttacga cttttgcaaa
     8161 gggcatcata aaaaacactt tttccctttc ataaagtttc aggtagtcag atactccggg
     8221 aagatatttt ctgtcagcct ttgataccac ccctttttca aggaatacgt caaagtcatg
     8281 cctgaatccc tttgctttgg cttcggcttt ttccatggct tttgatgggt cgtctattat
     8341 tacagtgcct ccgtgcatgt aatcccatat gtattcgggg gatttgtaga aataatctat
     8401 atagttctca agatgctgca tgtttgttct tgtgatcaca gcttccttaa gctgctctct
     8461 tctttcatcc atatcagcat aatttttttc tattttccgg agggcatcat catatatcga
     8521 aggacctccg actatctggc gggccggata aaggctcacc atatggattt tcttgaggga
     8581 tctctggctc tcgggatcaa acagtcttat ggattctatt tcatcatcaa aaaaatcaat
     8641 tctgtaagga agttcgttat atggagcaaa gatgtctact attcctcccc gtttactgta
     8701 ctggcccttc gcatatacca ttggtactct ttcataaccg gtttccgtca gtctttcttc
     8761 cagaaggtgc acatccatgg tttctccggt ccttagcact gaaacgcttt caagaaatgt
     8821 gctatggggg ggcagcttct ttatgacccg gctcacaggt gccacaacta tacatctgtc
     8881 cccttccgcc aaagtcttta atatgttcag tctttctata agagaatcat tgttttttgc
     8941 ttcataattc aaaaaggtct cttcctcgga aggcataacg catatttttt catccgtgaa
     9001 aaaagaaagg tcagaagcca attgcccggc tctgacgaaa gtaggtgcca ctataaggaa
     9061 ctggccgccc ttttttttta ttatgtcggc acaaaccgca gcaactctgc tgtccgatat
     9121 gccggttata cttactattc ccattcttct ttctcgctga ccctgctgtt ataacggttc
     9181 attgcggcat ctatgccttt ttctattata cattctgtcg cttcggcggc agttatcacc
     9241 gcccccttca ggatctcctt ttcttttttg ttcatgcctc cggtgacaaa atccatcaga
     9301 tgcattttta tgtctccgcc aatacctatc cttatcctgg gaaattcctc tgtccccatg
     9361 gcggcaacaa ctgatttcat cccgttatgc gtcccggggc ctcccttttt tcttattctt
     9421 attctgcctg tttctatgtc tatatcatcg tatatcaaaa tcatcttctc cggctccact
     9481 ttgtaataat gcattatttc tctcaccgct tcaccgctca gattcatgta tgtttggggt
     9541 tttgccagca aaactttttg gccggcaaga tatccctcgc cgaccaatga cttgtgtttg
     9601 attttattga ttttgatgtc aagcttttca gcaaactcat cagcgacaat aaaccccata
     9661 ttatgtcttg tttttgcata ttttctgccg gggttgccca gacctacaac aacaaacatt
     9721 ttttgtcctt ctttcaatcg aaaagtttgc ttattgaatc atttgtatgt attctcatca
     9781 tggactccgc aaacaaaggt gcaacggaaa gcatcttgaa ttttttgtgc tttttttctt
     9841 ctgccatggg tatcgtattc agcaatacca cctcatcgaa gtcagaagca tccagccttt
     9901 cgatggccgg acccgaaagc accgggtggg tggcgcatgc agaaacactt tttgctccca
     9961 tctccataag ggctttgcct gcattgcata tggtccccgc tgtatctatc atatcatcca
    10021 cgagaacaca gtttttatcc tttatatctc ctattatatt cattatttcg cttttgtttg
    10081 gttcgggtct tctcttgtca actatggcta taggggcatc gaggggctct gccatgctcc
    10141 tggccctggt aacagaaccg tggtcgggag aaactatcac aagatccttt atgttttttt
    10201 tggtaaagta tttcaccagt attggaagac caatcagatg gtccacgggt atattgaaat
    10261 atccctgtat ctgtgcagca tgaaggtcca ttgttattac ccggtcggca ccggcggtca
    10321 caaggaggtc tgcaacgact ttcgccgtta tgggatctct cgcctttgct ttcctgtcct
    10381 gtcttgcata tccgtaatag ggcacaacag catttatcct acctgccgac gctcttttca
    10441 tggcatccat cattatgatc agctccatca gattgtcatt tacgggataa catgtggatt
    10501 gtattatgta gcaatccaca cctctcacgg tttcccacag attcactgat atttcaccgt
    10561 cactgaattt tgtcacattg gcttttccaa ggggcttacc cattatggat gttatctcat
    10621 cagccagttc aagatgtgaa ttcccagcaa atactttaaa atccgaaaac cctgcatttt
    10681 tcattactct tttacctcta ctttcatttt ttgagaatgc ctcttttttc tacccagcct
    10741 tccagcactc ttcctttgct tctttcaaca taaagagcgc cgccgggcac atcctttgtg
    10801 accgtgctcc ctgcggctat gtacgatcct tcttccagag taacggggga aacaagattt
    10861 acattacaac ctataaaagt tccgtctccc acctttgatc tgtatttttt gctgccgtca
    10921 tagtttacaa aaaccacacc gcatccgata ttgacatcct tccctatatc cgaatctccg
    10981 atataggaaa gatgggatgc tttgctgccg tctccgaaaa cagaattttt tatttcaaca
    11041 aaatcgccta ttctgcaacc gctgccaaca tcacttccgg gtctcagata ggcaaaaggt
    11101 cctatttttg tttctgtgcc tatcctgctt tctgtaatta cagaattctg tatctgtgtc
    11161 ctgtctccta tttcggactc ggtgatcctg gtacccggtc cgatcacaca gtcttctcct
    11221 atggtcacac tgccttcaag tatgactccg ggataaagga ccgtgccctt ttttatatcc
    11281 actttatttc cttctatata gacctgcttt atatctatga aacgcacgcc cttttctgca
    11341 aaaaagaggt tcttccttat gcgttctttt tcctgttttt cgtattcttc aaaattcatg
    11401 agatcttttc tcccctgcct gtaacagcat ctttttcgag tatcatttct cccgccctgt
    11461 aaacatcccc ggcgcccatg gttattatca gatctcccgg ctgggcattg ttatatacga
    11521 aactggcgat ctcttcaaga ctgccgaaat agtaaacttc tttttcgggg taggattttt
    11581 ttatttctgc catcagatcc tttgagctta tctgataaac gtttttttct ctggcggcat
    11641 atatctctgc cagcaccacc ttgtccgcct tttcgaagga ggccgggaac tcgtcaaaaa
    11701 gtgccagagt cctggtatat gtgtgaggct ggaaaataca ccagatattt ttgtgtggcg
    11761 tatttatagc agcttctatg gtggctctta tttctgcggg atgatgggca taatcatcaa
    11821 cgatccttat cccggactga gtctctccca gaacatcgaa tcttctctgg gtccccttgt
    11881 atttttcgag ggtctctttt atatcattga tatctactcc gagaacactg cagcatgccg
    11941 ttgccaccag cgcattcagg atgttgtgct ctcccggtat ggacaactgc agcttgcaga
    12001 gttttttgcc tttgcttgat atttcgaatg cgggcatccc gtcggcattg aactctatct
    12061 cctttgcata atagtcgcac ttatcattga agccgaaggt tattacatta ggaagatcct
    12121 gtattattct gtttacaaag ggattggcat cataagcaaa aatcattccg tcttccggca
    12181 ccagtgccgc gaatttctca aaagaactgg cgatatgctc aatgtctttg aaataatcaa
    12241 ggtgatccga atctatattg agtattatct ctatcctggg tttgagttcc agaaaactgt
    12301 ccatatattc gcaggcttcg gtgatgaaga actggctttt gcccaccttt acattgccgc
    12361 cgatctcaga aaggttcccc cccacaagta tagtcgggtc tttttttgct ttttcaagta
    12421 taagggatat catggaagtg gtggtggtct tgccgtgagt tccggcaaca gctatgctgt
    12481 ttttgcatcc tttcatgaga tgccccagca tctgagccct tgaaacgcag ggtatgccca
    12541 tctccttgcc tcggaccatt tccgggttgt ccttggtgac agccgccgag tagactatga
    12601 tatctgctcc ttccacattt ttggctctgt gtcccagaaa aatgtttgcc cccgccttta
    12661 tcagctgctc agttttatca ttctgattca tgtcggaacc tgtcacaaga tatccttcag
    12721 acaataaaat acctgctatt gccgacaggc ctactccacc tatcccaatg cagtgcactt
    12781 gtttaacatt tgccaagtcg atcattaaaa atcctccgat gtttttatgc caagattata
    12841 acatactttg ctgttattat atatggattt gacaaaaata agtaaaacga ataatatcat
    12901 aatatctttc aattattgtt cgtaaaggag tgaataatga aaaggaccga gcgcatcggc
    12961 gccatcataa aaatactctc tgattctccc agcaggccct tcggtctaag ctatttctgt
    13021 gatcttttag gggctgcgaa atccagtata agcgaagatc tgaaatcggc cagagaaatt
    13081 gtgagcagca tggaactggg tcttatagaa accattcccg gcgccagagg aggcgttatt
    13141 ttcatacctc atatatacga tgaccagtgc cttgctctgc agcaggaatt atgcgacctc
    13201 ataaaggacc ctttcaggat gctgggaggc ggatttttat atacctcaga tataatgttc
    13261 aatgcatccc ttacaaagaa aatggctctg gtctttgccc ggaaattcag aaattccggt
    13321 gctgaatatg ttgccacaat tgagacaaag ggtatccctc ttgcactaat gacatcctgg
    13381 atgctaaaca ttcccgcggt ggtcgtaaga agagaaac
//
LOCUS       DAONHK010000016        13241 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_3671_length_13241_cov_4.342939, whole genome shotgun sequence.
ACCESSION   DAONHK010000016 DAONHK010000000
VERSION     DAONHK010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 13241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13241
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3671_length_13241_cov_4.342939"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(79..1758)
                     /locus_tag="PLM92_01240"
     CDS             complement(79..1758)
                     /locus_tag="PLM92_01240"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HPR23799.1"
                     /translation="MREKKQNREKGKLRVIPLGGLNEIGKNMTLLESGDDMMIIDCGL
                     SFPGDEMFGIDIVIPDFSYLKENRNKIRGIIITHGHEDHIGSTPYLLKELNVPIYATR
                     LPLALIKSKLEEHGLSGKLNAIEAGSKIRLGAFKVEAIRTTHSIVDSVCLCIDTPAGR
                     VFHTGDFKVDYTPVDGEPIDFARLAELGTKGIMFMMSDSTNATRPGYTSSEKTVGQAL
                     ENIFRRSRSRIIVATFSSNVHRVQKIIDNAVLFKRKVAFSGRSMVKVANLAAELGYLR
                     IPSGTLVDINKIKNMPDSELVIITTGSQGEPMSALTRMAAADHRAVQIKKGDMIILSS
                     TPIPGNEKTVSDVVNKLIQKDAEVIYSDIADTHVSGHACSEELKLMLSLIKPKYFMPV
                     HGEHRHLKAHADIAQSMGIKKENIFILENGNILDITEKNAYIKKEKVEADAVMVDGLG
                     VGDVGNIVLRDRRLLSESGLIIVVASVNRATRQVVAGPDIISRGFVYVRENEHLIDQA
                     TEVVSNTLKNCFDKNKKDWNEMKSAVRDDLRKFIYEKTKRNPVILPIFMEV"
     gene            complement(1769..4237)
                     /gene="leuS"
                     /locus_tag="PLM92_01245"
     CDS             complement(1769..4237)
                     /gene="leuS"
                     /locus_tag="PLM92_01245"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006000736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HPR23800.1"
                     /translation="MKQKYDFSTIEAKWQKNWEDTDAFAVYEDETKEKYYVLEMFPYP
                     SGKLHMGHVRNYSIGDVVARFKTMNGFNVLHPMGYDAFGLPAENAAIKNKVEPARWTD
                     DNIDEMTRQLKQMGISYDWNRTIATCRPDYYKWMQWIFIQFYNKGLAYKKENPVNWCP
                     SCKTVLANEQVIDGRCERCDTEVGKKELSQWYFRITEYAESLLENLETLDGWPEKVKT
                     MQKNWIGKSEGAEVTFEIEGYDKGLDIFTTRPDTLFGVTYMVLAPEHPYLKDLVTGSA
                     YEKEVLDYVEKVQHMSDIQRTAATGEKTGVFTGRYCINPVNGKRVPVYISDYVLMGYG
                     TGAIMAVPAHDQRDFDFAKTFGLEIIPVVDTEDPGIDVYDLKEAFAAEGNMINSGIFD
                     GMNNKEAILKITGHLEEKGIGKKDINYKLRDWLISRQRYWGTPIPMIYCEDCGWVPEK
                     EENLPVMLPTDVEFTGKGESPLSTSKEFASVFCPNCGKPATREMDTMDTFLDSSWYFL
                     RFCDARNEKEAFSYEKVKYWMNVDQYIGGVEHAILHLMYSRFFQMALYDCGLVSTREP
                     FRNLLTQGMVIKDGKKMSKSLGNVVSPSEIIEKYGADTARLFILFASPPERELDWSDA
                     GVEGSFRFINRVYRMVYEFTEKYGCGHADNNLENERDKELLYKMNATVKKVTEDIDER
                     FNFNTAISAVMELVNEMYKYKEGTINEKLFRGAIEELILILAPFTPHLCEELWRQIGK
                     ENSVHKMKWPKYDEAALKKDTVEIVVQLNGKVKQKLNVTNNISKEELEKTAMNDTGVK
                     KVLENRDIIKIIAVPNKLVNIVVR"
     gene            complement(4221..4571)
                     /gene="rsfS"
                     /locus_tag="PLM92_01250"
     CDS             complement(4221..4571)
                     /gene="rsfS"
                     /locus_tag="PLM92_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006000738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HPR23801.1"
                     /translation="MNSKKTALLAAEILDGKKASDVIIIDISDKSSFADYFVIASGRS
                     ERQIGTLADYAEEGLAGKGITAKNIEGKKESGWILMDYGDIIVNVLTEEMREKYNIES
                     IWADCEVIRNEAEI"
     gene            complement(4537..5139)
                     /gene="yqeK"
                     /locus_tag="PLM92_01255"
     CDS             complement(4537..5139)
                     /gene="yqeK"
                     /locus_tag="PLM92_01255"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: protein motif:HMM:TIGR00488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HPR23802.1"
                     /translation="MIFTNDHTIEKYSIKRKEHTEEVRKLAVALAKRYGADTEKAEKA
                     AVFHDMFRGKGKDILDVYVREMGLPERYIQNPDLSHSKIAAFLMEKELDIRDEDVLNA
                     VKYHTTGRANMSKLEKVIFLADSIEPGRDYPGIEKIRELSNKDLDAACLSAIENTIEY
                     LHRQDINIDPDTEKAREWLQKTISKKGDLDEQQKNSITCG"
     gene            complement(5123..5728)
                     /gene="nadD"
                     /locus_tag="PLM92_01260"
     CDS             complement(5123..5728)
                     /gene="nadD"
                     /locus_tag="PLM92_01260"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HPR23803.1"
                     /translation="MKRIGVLGGTFDPIHLGHLSLARDAIFQAKLDKVIFVPAGKQPF
                     KLWNDISSAAERMDMLRLAVLDEKDMEVSGVEIDSEEISYTYKTLRALRNKMDSGDKL
                     YFITGTDTFLKLETWKEAEALLRENAFVVGVRPGYIKKEITAFKKHIEKVFYTEAIII
                     NNIRLDISSTEIRKRVKNGEDISGMVKKEVERYIVKNDLYK"
     gene            complement(5730..6014)
                     /locus_tag="PLM92_01265"
     CDS             complement(5730..6014)
                     /locus_tag="PLM92_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HPR23804.1"
                     /translation="MNQKDKGEKERICQSCAMPMGQAVEFYGTNKDGSKNLDYCKFCY
                     QNGSFTEDQSMDEMIEIAAGYMAKYNENMSLQTARRLMQGLFPSLKRWKK"
     gene            complement(6011..7789)
                     /gene="aspS"
                     /locus_tag="PLM92_01270"
     CDS             complement(6011..7789)
                     /gene="aspS"
                     /locus_tag="PLM92_01270"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006000744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HPR23805.1"
                     /translation="MDGLFKRTNMCGSFRIKDIGRETVINGWIQKRRNLGGLIFCDVR
                     DRTGIVQVVFDDNIPKELFERADSLRSEFVVGIKGEVRERQAKNPDIPTGEIEIFVND
                     LVVYSKAETTPIYIKDDDNAEDNLRLKYRYLDLRKPSMQKNLILRHKITKLARDYFDE
                     QGFIEVETPMLIKPTPEGARDYLVPSRISPGNFYALPQSPQMFKQLLMVSGIDRYFQI
                     ARCFRDEDLRADRQPEFTQIDMEMSFAEMDDIIKIQEGFLKRMLKEIKGKDIKTPFPR
                     LTYEEAMERYGSDKPDTRFGNELKKLNETVKNCSFDVFTKALQKGGDVRGICIEKGGE
                     HFSRKDIDRLTQAVKSYGASGLVWMKKSGEGITSSVNKFFSIEQLTEIADVFSAKEGD
                     LILIVADSKSVTLDSLGFLRKYTAEVMGLLDDDKYELLWVTDFPLFEYDEEEKRFKAK
                     HHPFTAPDQKDVERLESHPESVNAQAYDIVMNGVELGGGSIRIHDRGLQEKMFKALGI
                     SEEEARSKFGFLVDAFKYGAPPHGGLAYGLDRLVMLIAKEKSIREVIAFPKNQAAQCM
                     VSGAPASADQRQLDELSIEINREKKI"
     gene            complement(7790..9046)
                     /gene="hisS"
                     /locus_tag="PLM92_01275"
     CDS             complement(7790..9046)
                     /gene="hisS"
                     /locus_tag="PLM92_01275"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007789204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HPR23806.1"
                     /translation="MLTNAPRGTKDMLPEDAYKWHYIEEAFADICRRYGFREIRTPVF
                     EHTELFTRGVGDTTDIVQKEMYTWNQSDRSLTLRPEGTSPVVRAFVEHKMYADPQPSK
                     YYYNIPCFRYEKPQSGRQRQFHQFGIEIFGTNDMMADTEVIALGKEFIEGLGIRDVEL
                     QINSIGCPKCRDSYRNALREYLKPKYDQLCDTCKDRYERNPMRILDCKSPADQELVKD
                     APLMIDYICDECKTAFTDLKADLDSMGIEYVVNPGIVRGLDYYTKTAFEFVALNIGSQ
                     GTVCGGGRYDHLSEELGGPPIPGVGFGLGKERLLMLMESNGVEIPKPMGADAFIAFVG
                     EDSKASALKLMRGLRKEGLHIEMDCMARNIKGQFKYADRLGVRKTVIIGENELALGQV
                     TIKDMETSKQKQIPFGEILTELKKEK"
     gene            complement(9072..9692)
                     /locus_tag="PLM92_01280"
     CDS             complement(9072..9692)
                     /locus_tag="PLM92_01280"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPR23807.1"
                     /translation="MEILNMPSGPLMVNTYLVIDEKNKEGFIVDPGGYDTGLIGEIRK
                     KQTDIKYIILTHGHGDHIGGVKGYRKYMPGAKVVASAAEKELLADASMNMSMMTQNEA
                     IEVHPDMLVDEGDELFVGDMKLRFIMTPGHSPGGMCIHTENALFSGDTLFHSSVGRTD
                     FPGASFEELSKAIHEKLFVLPEETMVYPGHMGPTSIGFEKKNNPFL"
     gene            complement(9707..11890)
                     /locus_tag="PLM92_01285"
     CDS             complement(9707..11890)
                     /locus_tag="PLM92_01285"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006000753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HPR23808.1"
                     /translation="MEGKQDFLDHILKMNPKYNIDYIGKAYDVAEKVHDGQLRRSGEP
                     YVTHPVEVAKILAELGMDEETLAAGLLHDAVEDTSYTEKELIDDFGEGVGLLVDGVTK
                     LGSIVFDNKEERQAENMRKMFLAMSKDIRVLIIKLADRLHNLRTINYLNEEKIQDKCR
                     ETLDIYAPLASRLGIYAMKFELEDMAFKHLYPDAYEQLTGNVAAKKEERQTALDHVIE
                     EIEEALDDLGIHYDVSGRSKHFYSIYKKMEFQKKQLDEIFDLMAVRVIVDSVKDCYSV
                     LGIVHTMWTPIPGRFKDYIAMPKPNMYQSIHTTVIGESGKPFEIQIRTYDMHRIAEYG
                     IAAHWKYKEGVVNDQEDVKLAWLRQTLEWQKDMEDPTEFMESLKVDLFENQVFVFTPQ
                     GDVIELPSGSTPLDFAFKIHTDVGAKCVGAKVNGKMVPIDHKLENGTIIEIITSPNAK
                     GPSIDWLKIAQSSTARNKIRQWLRKENKSDDIDKGKDNIDKSIRKKGYDPKQVLINSY
                     ITKYIKEVGLTGTDELYTQLANGGTYVSRVAAAFIKYYTEDKKEEQKKQKNEAADIID
                     EKAKKEKQRSRDNPGVKVKDMDNLLIRVSKCCNPVPGDEIIGFITKGRGISVHRTDCD
                     NIVALPVSEKARLIQVEWDDSKEKSYNADISIIAEDRKGLFSDLSKACEAMDVHIAGV
                     NARSGKDRSIDVILTLSISNTAQMKKILRALKNVQGVSEVYRTRS"
     gene            complement(11928..>13241)
                     /gene="secF"
                     /locus_tag="PLM92_01290"
     CDS             complement(11928..>13241)
                     /gene="secF"
                     /locus_tag="PLM92_01290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HPR23809.1"
                     /translation="KGFAWTLMLGIIASIFTAVVVTQLYLNIIAGTKTFGKNKFFGIK
                     DDGTIAFGIKRHFRFISRRKIFYVISVLVIAAGFLVGGIRGLNYGIDFTGGTMMQFDM
                     GRQVTTEEIKDVMKDHDIDVNQMEVVFSGNDKEEVVIRTNEALENRQRAQVAEDFQKT
                     FDLTDDDVLATELFGPSVGKELRNNAIKAILLAALGMLIYIRLRFRKWKFGAAALAGV
                     VHDVLWVLAFYAIFQVTVNNPFIAAILTVVGYSINDTIVVFDRIRENNRFMRKESEEE
                     IIDASINQTLTRSVMTSMTTLVVLIPLYIMTTSSIREFVLPLMVGIIVGMLSSIFVCS
                     PLYYEFSKLGSRKHYKSAASKGGKKKKYEKIESTKKRPERKPAEEAPEEIAMPEKEPE
                     EVEYIVEVEEDILSEEEPAKEVQTNKSKKNKNRSKKSKKSKKKKR"
BASE COUNT         3057 a         3205 c         2287 g         4692 t
ORIGIN      
        1 tccctgtatt ccatcgattc tttgatggct gcttcaagtc tgtgtgcttc atcgtaaacg
       61 ttcatttgtt atctcctttc atacttccat gaatattgga aggataacgg gatttctctt
      121 ggttttttcg tatatgaatt ttctcagatc atcgcggacc gcagatttca tttcgttcca
      181 gtcttttttg tttttgtcaa aacagttttt aagtgtattt gaaacaacct ctgttgcctg
      241 atcaataaga tgttcattct cacgaacata aacaaatccc cttgatatta tatccggccc
      301 tgccacaact tgtcttgttg ctctgttaac ggatgccaca acaataataa gaccggattc
      361 agaaagcaac cttctgtctc tgagtacgat attgcccaca tcacctacgc ccagtccatc
      421 gaccattact gcatcagctt ctaccttctc tttttttatg tatgcattct tttctgtaat
      481 atcaagtata ttcccgtttt caagtatgaa tatattttct ttttttatcc ccatactctg
      541 ggctatatcc gcatgcgcct taagatgtcg gtgttctcca tgaacaggca tgaaatattt
      601 tggttttatg agggataaca taagtttgag ctcttccgaa catgcatgac ccgagacatg
      661 agtatccgca atatcggaat aaatgacttc cgcatccttt tgtatgagtt tatttactac
      721 gtcagaaacc gttttttcgt tgccgggtat gggtgttgat gaaagtatta tcatatcacc
      781 tttttttatc tgtaccgccc tgtgatctgc cgctgccatt cttgtcaatg cggacatggg
      841 ctctccctga ctaccggtgg taattataac caactcacta tcaggcatat ttttgatttt
      901 atttatatcc acaagcgttc ccgaaggtat ccttaaatat cccaattcag cggcaagatt
      961 tgctaccttt accatacttc ttccggaaaa agcaactttt cttttgaaaa gcactgcatt
     1021 gtcgataatc ttctgtactc tatggacatt cgatgaaaat gtagcaacta ttatcctgct
     1081 gcgggatctt ctgaaaatgt tttccaatgc ctgtcccact gttttttcag aagatgtata
     1141 gccgggtctt gtggcatttg tactgtccga catcatgaac atgatgcctt ttgttccaag
     1201 ttccgctaat ctggcaaaat caattggttc gccgtctaca ggtgtataat cgaccttaaa
     1261 atcacccgta tgaaaaactc tgcctgcagg cgtatcaata caaagacata cagagtcaac
     1321 tatggaatgt gtggttctta tggcttcaac tttaaaagct cccagtctta ttttgcttcc
     1381 ggcttctatt gcgttaagtt tgccggaaag accgtgctct tccagtttgc tttttataag
     1441 tgccaatgga agccttgttg cataaatcgg aacattcaac tctttaagca gatatggtgt
     1501 tgatcctatg tgatcctcat gtccgtgggt tattataatg ccccttattt tatttctgtt
     1561 ttctttcagg tatgaaaagt caggtataac tatatctatt ccgaacattt cgtccccagg
     1621 aaatgaaaga ccacagtcga ttatcatcat gtcatcgccc gattccaaaa gggtcatgtt
     1681 ctttcctatc tcattcagtc cgcctaaggg aatcaccctc agcttacctt tttctctatt
     1741 ttgttttttc tctctcatat tttttctttt atctgacaac tatattgacc agcttattgg
     1801 gaacagcgat gatcttaatt atatccctgt tttccagcac ttttttcaca ccggtatcat
     1861 tcatagcagt cttttcaagt tcctccttgc taatattatt tgtaacattt agtttctgtt
     1921 tcacctttcc gttcagttgc acaacgattt cgacggtgtc tttttttaaa gctgcttcat
     1981 catatttggg ccatttcatt ttgtgtactg aattctcttt gcctatttgt ctccacagct
     2041 cttcacacaa atgtggtgtg aaaggagcca gtataagtat aagctcttca atagccccac
     2101 ggaaaagttt ttcatttatt gtgccttctt tatatttgta catttcgttg accagttcca
     2161 ttacagcact tattgctgta ttgaaattga atctttcgtc gatgtcttct gtgacttttt
     2221 ttactgtagc attcattttg tataaaagct ctttgtctct ttcgttttca agattattat
     2281 cggcatgacc gcagccgtat ttttccgtaa actcataaac catcctgtat actctgttaa
     2341 tgaatctgaa gctgccttcc actcccgcat ccgaccagtc cagttcccgc tcggggggtg
     2401 aggcaaacag tataaacagt cttgctgtat ctgctccgta tttttctatt atttctgagg
     2461 ggctgacaac atttcccaga gatttggaca tttttttgcc gtcttttatt accatcccct
     2521 gagtaagcag attacggaaa ggttccctgg tactgacaag accgcagtca taaagtgcca
     2581 tctggaaaaa tctcgaatac atcaggtgca gtatggcatg ctcaacgcct ccaatatact
     2641 gatccacatt catccagtat tttacctttt cgtaactgaa agcttctttc tcatttcttg
     2701 catcacagaa tctcaggaaa taccatgaag agtcaagaaa tgtatccatt gtgtccattt
     2761 cccttgtggc cggtttgccg caattgggac aaaatacact tgcaaattct ttgcttgttg
     2821 acagggggga ttctcccttt ccggtaaatt ccacatctgt aggaagcatt acaggaaggt
     2881 tttcttcttt ttccggcacc catccgcagt cttcacaata tatcatgggg ataggtgtcc
     2941 cccagtaacg ctgccttgat atgagccagt cacgaagttt gtagtttata tcttttttgc
     3001 ctattccttt ttcttccaga tggccggtta tcttcaaaat ggcctctttg ttgttcatgc
     3061 catcaaagat gcccgaattt atcatattcc cttcagctgc aaaggcctct ttaagatcat
     3121 aaacatctat tccgggatct tcggtatcta ctacaggtat gatttcaaga ccgaaggttt
     3181 tggcaaaatc aaagtctctc tggtcatgag cgggtactgc cattatcgct cctgtgccat
     3241 atcccataag aacataatcc gagatatata ccggtaccct tttgccgttt acgggattta
     3301 tacagtatct tcccgtgaag actcctgtct tttcccctgt ggctgcagtt ctttgtatgt
     3361 cgctcatgtg ctggactttt tctacataat cgagaacttc tttttcatag gcgctcccgg
     3421 taacaagatc tttcagataa ggatgctcgg gagccaggac catataggtc actccaaaaa
     3481 gtgtatccgg tctggttgta aaaatatcaa ggcctttatc ataaccttct atctcgaagg
     3541 tgacctctgc accttcactt tttcctatcc agtttttttg cattgtcttg accttttcgg
     3601 gccagccgtc cagagtttcc agattttcca ggagactttc agcgtattcg gttatacgga
     3661 aataccactg tgaaagttct tttttcccta cttcagtgtc acatctctca catcttccgt
     3721 caattacctg ctcatttgca agtacggttt tgcagctggg acaccagttg acaggatttt
     3781 cttttttgta tgcaagtcct ttgttgtaaa actgtatgaa tatccactgc atccatttgt
     3841 agtaatccgg tctgcatgtg gcaatggtcc tgttccagtc atatgatatg cccatctgct
     3901 ttagctgcct tgtcatttca tctatgttgt catctgtcca tctggccggt tccactttgt
     3961 tttttattgc tgcgttttca gccggaagcc cgaaggcatc ataacccatg ggatgaagaa
     4021 cattaaatcc gttcattgtc ttgaatcttg caacaacatc tcctatggag taattcctca
     4081 catgacccat atgcagtttg cccgaaggat aagggaacat ttcaagtaca taatactttt
     4141 ctttggtttc atcctcataa acagcaaatg catcggtatc ttcccagttt ttctgccatt
     4201 ttgcctctat agtgctaaaa tcatatttct gcttcattcc tgattacctc acagtctgcc
     4261 caaatacttt ctatattata tttctctctc atttcttcgg tgagtacatt tactattatg
     4321 tcaccgtaat ccatgagtat ccatcccgat tcttttttgc cttcgatatt ttttgcagtt
     4381 atgcctttcc cggccagacc ttcttctgca taatcagcaa gggtgcctat ctgtctctcg
     4441 gatctccctg acgctataac aaaataatct gcgaaagatg atttatccga tatgtctata
     4501 atgataacat ccgatgcttt tttgccatcc agtatttcag ccgcaagtaa tgctgttttt
     4561 ttgctgttca tccaaatctc cttttttact tattgttttt tgaagccatt ccctggcttt
     4621 ttctgtatcc ggatctatat ttatgtcctg tctatgcaaa tattctattg tattttctat
     4681 ggctgaaaga caggcagcat ccagatcctt attggaaagt tctcttatct tttcgatccc
     4741 cggataatct cttccgggtt caatactgtc tgccagaaaa atcacttttt ccagttttga
     4801 catatttgct cttcctgtcg tatgatattt tactgcattg agtacgtcct cgtccctgat
     4861 atccagttct ttctccatca aaaatgctgc tattttgctg tgggaaagat ccgggttttg
     4921 gatatacctt tcaggcaatc ccatttccct gacatacacg tcaagaatat cttttccttt
     4981 gcccctgaac atatcatgaa aaacagcggc tttctcggct ttttctgtgt ccgctccgta
     5041 tcgttttgcc aaagccaccg ccagcttcct gacttcttcg gtgtgctctt ttctttttat
     5101 tgagtatttt tctatagtat gatcatttgt aaagatcatt ttttactatg tacctttcca
     5161 cttctttctt gaccattccg gaaatatctt ctccgttttt gacccttttt cttatttctg
     5221 ttgatgaaat atcaagtctt atattattaa ttattattgc ctctgtgtaa aacacttttt
     5281 caatatgttt tttgaaggca gttatctctt tcttgatata tcccggtctc acgcctacca
     5341 caaatgcatt ttccctcaaa agggcttccg cctctttcca tgtttcaagc ttaagaaaag
     5401 tatctgttcc tgttatgaaa tacagtttgt ccccggaatc cattttattt ctaagagccc
     5461 ttaaggtttt gtatgtatag gatatttctt cagagtcgat ttccacaccg gagacttcca
     5521 tgtctttttc atccaaaacc gcaagtctca gcatatccat tctttcagcg gcactgctta
     5581 tatcattcca gagcttgaaa ggctgctttc ccgcaggaac aaaaattacc ttgtcaagtt
     5641 ttgcctgaaa aatcgcatct cttgccagag aaagatgccc cagatgtatc ggatcgaatg
     5701 tgcctcccag aactcctatc cttttcattt tattttttcc acctttttaa tgaaggaaaa
     5761 agtccctgca tcagcctcct ggcagtctga agagacatat tctcattata cttagccata
     5821 tatcctgctg ctatttcaat catttcatcc atgctttggt cctctgtgaa acttccgttc
     5881 tgataacaga atttgcagta atccagattt ttgcttccat ctttgtttgt tccgtaaaat
     5941 tccaccgcct ggcccatggg catggcacat gactgacata tcctctcttt ttctccttta
     6001 tctttttgat tcatatcttt ttctcccggt ttatttctat tgaaagctca tcaagttgtc
     6061 tctgatctgc cgatgcaggt gcgccgctga ccatacactg ggccgcctgg ttctttggga
     6121 atgctataac ttcccttatg cttttttctt ttgcaatcag cataaccagc ctgtcaaggc
     6181 cataggccag acctccgtga ggcggtgccc cgtatttgaa cgcatcaaca agaaatccga
     6241 atttgctgcg ggcctcttcc tcacttattc ccagagcctt aaacatcttt tcctgaagtc
     6301 cgcggtcatg tatccttata cttcctccgc ccagttcaac accgttcata actatatcat
     6361 atgcctgtgc attaacactt tcaggatgac tttccagtct ttccacatcc ttttggtcag
     6421 gtgcagtaaa aggatggtgc tttgctttga atcttttttc ttcctcatca tattcaaaaa
     6481 gaggaaaatc cgttacccac aaaagctcat atttgtcatc gtctaaaaga cccattacct
     6541 ctgctgtata ttttctcaga aatcccagac tgtccagagt tactgacttg ctgtctgcaa
     6601 ctatcagtat gagatctcct tcctttgccg agaaaacatc ggcgatttcg gtaagctgct
     6661 ctatgctgaa gaacttattg acggaagaag ttatgccttc cccgcttttt ttcatccaaa
     6721 ccaggcccga tgctccgtaa ctctttactg cttgagtaag tctgtcaata tcttttctgc
     6781 tgaaatgctc cccgcctttt tctatgcata tgcctctaac gtctcctccc ttttgaagtg
     6841 ctttcgtgaa aacatcaaaa ctgcagtttt ttacggtttc attcagtttc ttcagttcat
     6901 tgccgaatct ggtgtcgggt ttatcgctgc cgtaacgctc cattgcttct tcataggtca
     6961 atcttggaaa gggggttttt atatcctttc cctttatttc ctttagcatt cttttaagaa
     7021 aaccttcctg tattttgatt atatcatcca tttccgcaaa ggacatttcc atatctatct
     7081 gcgtaaattc cggctgcctg tcagctctca gatcctcatc tctgaaacat ctggctatct
     7141 ggaaatatct gtcgatgccg ctgaccatta ggagctgttt gaacatctgt ggtgactgag
     7201 gcagtgcata gaaatttccc gggcttattc tgctgggcac aagataatcc ctggcccctt
     7261 ccggtgtggg tttgatcaac atcggagtct caacttcaat aaagccctgc tcatcaaaat
     7321 aatctcttgc caattttgtt attttatgcc tcaaaatcag gtttttctgc attgaaggtt
     7381 ttctgagatc cagatacctg tatttcaatc tcagattatc ttctgcattg tcatcatcct
     7441 ttatatatat gggcgttgtt tccgccttgg agtatacaac aagatcattg acaaatatct
     7501 cgatttctcc cgtgggtatg tccggattct tggcttgtct ttctctgact tctcctttta
     7561 ttccaaccac aaattcgctc ctcaaactgt ccgccctttc aaaaagctcc tttggtatat
     7621 tatcatcgaa caccacctgc actatgccgg tcctgtctct tacatcacag aatataagtc
     7681 cccccaggtt tcttcttttc tgtatccatc cgtttataac ggtctctctg ccaatgtctt
     7741 ttatacggaa actcccgcac atatttgttc ttttgaataa tccgtccatt tatttctcct
     7801 ttttaagctc cgtgagaatc tctccaaagg gtatctgctt ttgctttgaa gtctccatat
     7861 ccttgatcgt cacttggccc agagccaatt cgttttctcc gattatcact gttttccgaa
     7921 ctcccagtct gtcggcatat ttgaactgac cttttatgtt tctggccata cagtccattt
     7981 caatatgcag tccttctttc cttagacctc tcataagctt cagtgctgat gcctttgaat
     8041 cttctcccac gaatgcgatg aatgcatctg cccccatggg ctttggtatt tccactccgt
     8101 tggactccat cagcataagg agtctttcct ttccaaggcc aaagccgact ccaggtatgg
     8161 gcggacctcc cagttcttca ctcagatggt cgtaacgtcc cccaccgcaa acggtgccct
     8221 gggaaccgat attcaatgca acgaattcaa atgctgtttt tgtgtaatag tccagccctc
     8281 ttactattcc cgggttgacc acatactcaa tacccatgga atcaagatcg gctttcagat
     8341 ctgtgaaggc cgttttacat tcatcacaaa tataatcgat cataagcgga gcatctttta
     8401 ccagctcctg atctgcagga gatttgcagt ccagtatcct catgggattt ctctcatatc
     8461 tgtccttgca tgtatcacaa agctggtcat atttcggctt aagatattcc cgaagagcat
     8521 ttcggtaact gtctctgcat ttcggacatc ctatactgtt tatctgaagt tctacatctc
     8581 ttattcccag accttcgatg aattcctttc caagggctat tacctccgta tcggccatca
     8641 tatcatttgt gccgaatatt tctattccga actgatggaa ctgtctttgc ctgccggatt
     8701 gaggtttttc gtatctgaag cacggtatat tataataata cttggaaggc tgagggtccg
     8761 catacatttt gtgctcaaca aaagccctta ccaccggcga tgtgccctca ggccttaatg
     8821 tcaggctacg gtctgactga ttccaggtat acatttcttt ctgaacgata tcagtagtat
     8881 ctcccactcc ccttgtgaaa agttcggtat gctcaaaaac cggtgtacgt atttctctga
     8941 acccgtatct tctgcatata tccgcaaaag cctcttcaat gtagtgccat ttatacgcat
     9001 cctccggcag catgtctttt gtccctctgg gtgcatttgt aagcattttt atctcctcct
     9061 gttagttttc tctataagaa aggattgttc tttttttcaa acccaatact tgttgggccc
     9121 atatgtcccg gatataccat ggtttcctcc ggaaggacga acaatttttc gtgtatagct
     9181 tttgaaagtt cttcaaatga tgcccccgga aaatccgttc tgcccacaga tgaatgaaaa
     9241 agagtatctc cgctgaaaag ggcattttct gtatgtatgc acatacctcc cggcgaatgt
     9301 cccggtgtca ttatgaaccg cagtttcata tcaccgacaa aaagttcgtc tccttcatcc
     9361 accagcatat cagggtggac ttctatggcc tcattctgtg tcatcattga catattcatt
     9421 gatgcatcgg ccagtagctc cttttccgcc gctgatgcca cgactttggc tccgggcata
     9481 tacttcctgt atcctttgac gcctccgata tggtctccgt gaccatgagt aagtattata
     9541 tactttatat cagtctgctt ttttcttatt tctcctatca gccctgtatc atatcccccc
     9601 ggatccacaa tgaatccctc tttgtttttt tcatcgatta ccagatatgt gtttaccatc
     9661 aaaggtccgc tgggcatgtt taaaatttcc atttgctttt gatctcctaa cttcttgttc
     9721 tgtatacttc cgaaacgccc tgaacatttt tgagtgctct taatatcttt ttcatctggg
     9781 cggtatttga tatggaaagg gtaagtatca cgtctatgct tctgtcttta ccgcttcttg
     9841 catttacacc ggctatatgg acatccattg cctcacaggc ttttgaaagg tcggaaaaaa
     9901 gaccttttcg atcttctgca attatggaaa tgtctgcatt gtaggatttt tccttgctgt
     9961 cgtcccattc cacctggata agtcttgctt tttcagaaac cggaagtgct actatattat
    10021 cacaatcagt cctgtgtacg gatatacctc tgcccttagt tataaatcct attatctcat
    10081 ctccgggaac agggttacaa catttggaaa ctctgatcag cagattgtcc atatccttta
    10141 cttttacccc cggattgtct cttgatctct gtttttcttt ttttgctttt tcatctatta
    10201 tgtccgcagc ttcgtttttc tgttttttct gctcttcttt cttgtcttcc gtataatact
    10261 ttatgaatgc agctgcaact ctactgacat atgtgccacc gttggccagc tgagtataaa
    10321 gctcatctgt ccccgtaagt ccaacttctt ttatgtactt tgtgatatat gagtttataa
    10381 ggacctgctt gggatcatat cctttttttc ttatgctctt atcaatatta tccttgccct
    10441 tgtcgatatc atcggatttg ttttcttttc tgagccactg tcttattttg tttcttgctg
    10501 tgctgctttg agctatctta agccagtcta tactggggcc tttggcattt ggtgatgtta
    10561 ttatctctat tatagttccg ttttcaagct tgtgatctat gggaaccatc ttcccgttca
    10621 ccttggcacc tacacatttt gcacccacat ccgtatgtat cttaaatgca aaatccaggg
    10681 gtgtggatcc tgaaggaagt tctattacat ctccctgggg agtaaaaaca aaaacctggt
    10741 tctcgaaaag atccactttc agagactcca tgaattccgt cggatcctcc atatctttct
    10801 gccattcgag ggtctgtctc agccatgcaa gcttgacatc ctcctgatca tttaccacac
    10861 cttctttgta tttccagtgt gcggcaatac cgtattccgc tatgcgatgc atatcgtaag
    10921 ttcttatctg tatctcgaag ggctttccgc tttctcctat taccgttgta tgtattgact
    10981 ggtacatgtt aggtttcggc atggctatat aatccttgaa acggccgggt ataggtgtcc
    11041 acatggtatg tactataccg agtacagagt aacagtcctt tacactgtcc actattaccc
    11101 gaactgccat aagatcaaag atttcgtcca gttgcttttt ctgaaattcc atctttttgt
    11161 atatgctgta aaaatgtttt gatcttcctg acacgtcata atgtatgcca agatcatcca
    11221 gtgcctcttc tatttcttct ataacatgat caagggctgt ctgcctttct tctttttttg
    11281 ccgccacatt gcctgtcagc tgctcgtagg catccggata caaatgcttg aacgccatat
    11341 cttccagttc gaatttcatt gcatatatac cgagtctgct ggcaagaggg gcataaatgt
    11401 caagggtttc cctgcactta tcctgtattt tttcctcatt cagataattt attgttctga
    11461 gattatgaag tctgtcggcc agtttgatta tcagtactct tatatctttt gacatggcca
    11521 gaaacatttt tctcatgttc tcagcctgtc tttcttcttt gttgtcaaaa acaatagacc
    11581 ccagctttgt gactccatcc accaaaaggc ccacgccttc tccaaaatca tcaatcagtt
    11641 ctttttctgt ataggacgtg tcttcaaccg catcatgcag aaggcccgca gcaagagtct
    11701 cctcatccat ccccagttcc gccagtattt ttgcaacctc aacaggatgt gtgacatatg
    11761 gctccccgga tcttcgtagc tgtccgtcat gcactttttc ggccacatcg taagctttcc
    11821 caatataatc aatattatat ttgggattca tttttagtat gtgatctaaa aaatcctgtt
    11881 taccttccat tttgaacttt aacgctttcc gctgatctca cctgaaatca tcttttcttt
    11941 tttttgcttt tcttgctttt cttgctccgg tttttgtttt ttttgctctt gtttgtctgt
    12001 acttcttttg ccggctcttc ttcggaaaga atatcttcct ctacttctac tatatattcc
    12061 acctcttccg gttctttttc gggcattgct atttcttcgg gtgcttcttc tgccggcttt
    12121 ctttccggtc gttttttagt gctttctatt ttttcatatt tctttttttt gcctcccttt
    12181 gatgccgcac ttttataatg cttcctgctt cccagttttg aaaattcata atacaagggg
    12241 ctgcatacga atatggatga aagcatgcct actattatgc ccaccataag aggcagtaca
    12301 aattctctta tggatgatgt ggtcataata taaagaggaa taaggacgac aagtgtcgtc
    12361 atggatgtca tgaccgatct tgtcagtgtt tgatttatgc tggcatctat gatctcttct
    12421 tcagattctt ttcgcatgaa cctgttgttt tctcttattc tgtcaaatac tacaatggta
    12481 tcatttattg aataccctac cacagtaagt atagctgcaa taaaaggatt gttgactgta
    12541 acctggaata tggcgtagaa ggccagtacc cagagcacat catgcaccac gcctgccagg
    12601 gctgcagcgc cgaatttcca ttttctgaag cggagcctta tatatatgag catgcccaat
    12661 gcagctaata atatagcttt tattgcattg ttcctgagtt cctttcccac cgatggtccg
    12721 aacagttctg tggccaggac atcatcatcg gtcaaatcaa atgttttttg gaaatcctct
    12781 gccacctgag ccctctgacg gttttcaagg gcttcgtttg ttcttataac gacttcctcc
    12841 ttatcgtttc cggaaaacac cacttccatc tgattgacat ctatgtcatg gtccttcata
    12901 acatctttta tttcctcagt ggtcacctgc cttcccatat cgaactgcat cattgtgccg
    12961 ccggtgaaat ctattccgta attaagacct cttatgcctc ccacaagaaa tcctgccgct
    13021 attactagaa cacttatcac atagaatatc tttcttctac tgataaaccg gaagtgtctt
    13081 ttgatgccga atgctatagt cccgtcatcc tttatcccga agaatttatt ttttccaaag
    13141 gtttttgttc ctgcaataat gttgagataa agctgggtaa ctactactgc cgtgaatata
    13201 cttgctatga tgcccagcat aagagtccat gcaaatcctt t
//
LOCUS       DAONHK010000017        13157 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_3696_length_13157_cov_4.585178, whole genome shotgun sequence.
ACCESSION   DAONHK010000017 DAONHK010000000
VERSION     DAONHK010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 13157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13157
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3696_length_13157_cov_4.585178"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      224..369
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            494..1633
                     /gene="metK"
                     /locus_tag="PLM92_01295"
     CDS             494..1633
                     /gene="metK"
                     /locus_tag="PLM92_01295"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006307349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HPR23810.1"
                     /translation="MKKLFTSESVTEGHPDKICDQISDAIVDAILASDPKGRVAAETS
                     VNTGYAMVMGEISTKCYIDIPKIVRQVICEIGYTKSEYGFDGDTCAVMTSIDEQSGDI
                     AMGVDPGGAGDQGIMFGYACNETKELMPMPISLSHKLARQLAKVRKNGKIKYLRPDGK
                     TQVTVEYDGDKVKRIDTVLISTQHDSDVKLSAIKRDLVEFVIKPVIPKKLMDKDTKIL
                     VNPTGRFVIGGPHGDSGLTGRKIIVDTYGGYGHHGGGCFSGKDPTKVDRSATYAARYV
                     AKNIVAAGLANKCEIQLAYAIGVAEPVSVMVDTFGTGRVPDEKLADIVVKHFDLRPKS
                     IMRNLDLRKPIYRQVAAYGHFGRSDLDLPWERTDKAEALKKELKK"
     gene            1621..2940
                     /locus_tag="PLM92_01300"
     CDS             1621..2940
                     /locus_tag="PLM92_01300"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003359029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HPR23811.1"
                     /translation="MEKMKVIKFGGSSAADDIQIAKIKNIVETDKSRRYVVVSAPGRR
                     FDQDSKITDILYSCFSSREQNVPYEQLFQVICDRFYAMEMNLGINVNMKAVLADIKEK
                     FQNGASLDYMASRGEFLSGKMIAEYIGYDFVDAADIIKFDKKGRLLEENTEEAIKDSL
                     KNHERAVIPGFYGSKPDGSIKTFPRGGSDITGALVASAMKADVYENWTDVSGFMVADP
                     RIIKDPKPISTISYMELRELSYMGASVLHEEAIYPARVANIPINIRNTNEPDNPGTMI
                     TAEPCEEANDRTVTGIAGSRDFAVIGINKNHLATERGFVRKLAGILEDNDVPLEHMPS
                     GIDTLSVVVSKKNVNGKLDDIMDEFEQQLKPDTMEIFEDMALIATVGCGMSFTPGVAA
                     RLFTALADAGVNIRMIDQGSSEMNIIVGVSNKDFEKSIKAIYKAFIE"
     gene            2956..4533
                     /locus_tag="PLM92_01305"
     CDS             2956..4533
                     /locus_tag="PLM92_01305"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HPR23812.1"
                     /translation="MRLRKRILSWVVVLALAVSVIAIDTDKADAASSRTYWIKVNKQA
                     NVATVYKRSGSKWKPIRAMLVTTGTNAWYAKEKMWCITPGGNFRIGIKMRWGEMMEEV
                     YAQYCSQIRGSILFHSVFYNKKNSPSSQASGQFYRLGSKGSHGCVRLCVMDAKWIYEN
                     CPRGTKVTVYSSKKAGPLGKPKRVGVKVYGWDPTDPNPKNPGFNMRRPVIKISKKKPA
                     TVQFGNSYSLKSKVTAKNTNANQSLTSKLKVSSVKKWKSGKWKKVKKFTTRSLGKYKI
                     TYKVRDKYCGKRGTAKKSFVVKVVDKKAPVIYAYNRTTKPGLSTAVTGVSAKQADGTS
                     RTAAMTVTVTGPQGSRTMSYAQARGYVLSREGVYTITYSVRNTYKPYKKAVRTIAVRC
                     YGDAVMSLTDPLPETITVDPDSGMIMIPAQEQAVEPETVDYITADKLKEEIGTIIRIR
                     DVNAAGTRSDKMLNTVLNYKNFISLSPILNEGENYITGDEVTATITYRGFNGTTISKP
                     ITIIIVEAPDPDPLPEK"
     gene            4606..4681
                     /locus_tag="PLM92_01310"
     tRNA            4606..4681
                     /locus_tag="PLM92_01310"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4639..4641,aa:Ala,seq:cgc)
     gene            5063..7870
                     /locus_tag="PLM92_01315"
     CDS             5063..7870
                     /locus_tag="PLM92_01315"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HPR23813.1"
                     /translation="MLLNTETTEKSGDDPNIKRRKQIHAIVLLIVILMCAVYLSFAYN
                     RHSNTMSSEAIMLGESLCSLLDTEHIKELSGDPGDTSSIDYKSTKQSLIRVIQEDNWL
                     HFAYIMSVKDGKMLFLLDSEPPDSPDYSPPGQIYEEATDEDWEPFRGEKTVLTKPVSD
                     RWGTWISTLVPIEDEKTGKVIASFGIDYDALEWNVRLWKKMIPDIIIVICFFALSMVL
                     MHTWNQQEVLRKLTRRLAVDEALYKGLFEQMPIGVSVMNDTNWYFGSEFKKININQKF
                     EDIMGRTRDQLKNLNWMDITYPEDLAEDVEKFRQFKSGEIKGYSMEKRFIKPDGSVIW
                     VRMTISPFIFSEDKRPLHLCLIEDITERKEIAETLKETERNKAVLFSNLPGMAFICKY
                     DRNWTMEFVSDGCYGLTGYESDSLVNNSKIAFNEIVMPEYRDILWQEAEKTLGNKMPL
                     KYEYEIITAEGEVKWVLMMAQAVYDEKEKIKSVEGIILDITDRKKLEQRLEYQSMHDE
                     ATGLHNRIYLEEVLRKEIKKDEKEYRALICLNLSTMNNLTLTYGFEYKYQLMKRITGS
                     LKVFCDKYHSLFITDETHLVFYVRGYKTGKEVLALCQNISEKLERVLEIERLGAGIGV
                     MEIDKDNRDDIDTILKDLMTVSEKALNTMNKEIGISFFDDEMKQQVFRDEEISRELSQ
                     IAAGERTERLFLQFQPIMDLDSKKICCFEALARMKSDNPKLIPPLEFIPIAEKTKLIV
                     PLGDHIIQKSLEFMKKLEANGHKDISVSINISAIQLLREGFAENLISVVDEMGITHGN
                     VALEFTESMFSSDFDYINEVLKKLRSYGIKTAIDDFGTGYSSFARERELNIDYLKIDK
                     FFIDKIMVLESKETITGDIISMAHKLGHGVVAEGVEYEMQFEYLKEHGCDKIQGYLVS
                     RPLDENVAIELLKEQKNMGWE"
     gene            7914..9689
                     /locus_tag="PLM92_01320"
     CDS             7914..9689
                     /locus_tag="PLM92_01320"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPR23814.1"
                     /translation="MDKKIYEEAIQKSIVAFAYHKGVFDDSGRMIDYIFIDMNPVFEK
                     YTGLKKEDIIGKRFADEIALDKEHAAEWVQRYAKVIAEQKEDRFEEYAKEYRRNFDII
                     AYPTDEKHFVTLFVNRTFEKKVQEIARFFIDNMGGHIDYDKLTEFAYEVSGAQYVAFN
                     LFDKNGKDFKTVSIYGETEIFNKNLELIKRHLLKRKWAHDPVKEQKTGRNDITVFENL
                     HDLTGEVIPQETVMQIERDYDIGDVVVAKITKERKVIGDFTLFFKKGKSRINHDLFKL
                     FLLQTGLFIEKTRLERSLFRSQKRFFTLAEYAPIGFMACDRKGNITYANKKLLEIMDS
                     PSYEATKSINLIEFVPLKKEGFSDKLVECMEKNREITYEMGYRSLWGKKTWLRVYYKP
                     FKTEEEVSGAHMVVDDITERKKEEDILKNMAERDALTKTFNRHALETVLIDRLNEAED
                     NDLVSCICIIDIDGFKMINDTYGHDVGDSVLKYLASRAKKELREKDLLIRTGGDEFLI
                     YFHDIKSEENTEDLVKRIFDKISTNYRFQDLNEENKLSLEVGCSIGVSLYPRHGRTIK
                     ELMAKADTALYEVKKSGKSNYHIID"
     gene            complement(9706..10644)
                     /gene="trxB"
                     /locus_tag="PLM92_01325"
     CDS             complement(9706..10644)
                     /gene="trxB"
                     /locus_tag="PLM92_01325"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002600954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HPR23815.1"
                     /translation="MDHNKYDTIIIGGGPGGYSAALYCARANLKTLIIEKLYPGGQMA
                     TTSMIDNYPGFDMGIDGVELAGKMKSGADRFGAETIFAEVTEVDMKGPDKTIKLADGK
                     VLHTKTIVIATGAAPRNLGLPYEKQLTGNGISYCATCDGMFYKEKKVVIVGGGNSAAA
                     DALILSKICKKVYLVHRHDTLKASPTYMAPLENASNLTFVWDSKIKEVMHDNKVTGVK
                     VENLKDGSVSEIACDGVFIAIGRMPNTEIFHKKIEMNPEGYIVADETTRTSEPGVFAV
                     GDLREKPLRQVVTATSDGAVASKFIEDYITHGFWKD"
     gene            complement(10905..11384)
                     /locus_tag="PLM92_01330"
     CDS             complement(10905..11384)
                     /locus_tag="PLM92_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPR23816.1"
                     /translation="MKFLENKLAEFNKTLPQIPENGKSVIANILWILIIISIVFNIIG
                     IITLLGVGSFGFSVLAGFGLGFHSMAIWGIVIFGVIGMAIVVVLEIIAIKPLKNKSYL
                     GWRLAFYVIWIQLIFSVLNDIFMGSIGGLIGVIIGTIIALFFLMQVRDYFIPDTEKS"
     gene            complement(11554..>13157)
                     /locus_tag="PLM92_01335"
     CDS             complement(11554..>13157)
                     /locus_tag="PLM92_01335"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HPR23817.1"
                     /translation="FVSDGCLELTGYAPDILYDSGDVTFNDIIAPEYKESVWTNRKQA
                     AENKEKLNFEYEITTIDSKRKWVLEMGECVYDEKGDVIALEGIILDITDRKNIEDHLK
                     YISEHDALTGLYNSYYLDLLLEKDSKKKYERKRAAISINLNTFYLLTAYSGYQYTQDM
                     IKNVAFALSEYCTDKRLLFKTSEGRFVFYLRDYKDKNELIDFTETILGLLRTLFIQDR
                     VSGGVGVVEIEQDQNNFDIDMIRRKLIIATENSIGIFDKDFKACFYNKELELEKNREK
                     EIRKALSTIAVNDQGGELFLQYQPILDLETESICAFEALARLKTDELGSVSPAEFIPI
                     AEKSKLIIPIGEKIILRALDFQKKLEEKGFEGIIIAINISVIQLLTPGFAEMLIEIIH
                     KKKINPKNVVIEITESIFEYDFDFINSVLVELKKSGLALAIDDFGSGYSSLAREMNLN
                     VNYIKIDKFFIDHILAADRPITGHIIEMAHKQGHYVVAEGVENIKQIQYLKEHGCDKV
                     QGFLISKPLDENAAVKFINEYVNNY"
BASE COUNT         4410 a         2476 c         2873 g         3398 t
ORIGIN      
        1 catggctccc atcagacggg tatctgtaac ataggctgaa acaaaatatt tttcggcctt
       61 ccacaaagga ggcagtccgc cttcgattat tctgagtgca ggtttattca tattaaaagt
      121 atacattata taaaagaaaa aacaagcaac agccaaaaaa gcctttcaag gcttgcaatg
      181 agccaaaaaa acatatataa ttatcatcgt aaaagttaca aaatccttat tcagagaggc
      241 tgagggaata ggccctgtga agcccggcaa cctggatgtc tgcaaacctt atagagaagt
      301 tgcagacaga caaaggtgcc aattcctacg gactgtatca caaacacatt caagtccgga
      361 agatgaggaa aagataagat gttatctgcc tcttctgcaa agaagaggtt ttcttaaaaa
      421 gggattttga cttaagtgaa tcaaaaaaaa catgaagaag gcggtattta ctgccggagg
      481 aggaaaaggc aagatgaaaa aactgttcac atcagaatct gttacagaag ggcatccgga
      541 caagatatgc gaccagatat ccgatgccat agtggatgcc atactggcaa gtgatccaaa
      601 ggggagagtg gcggcagaga caagtgtcaa tacgggatat gccatggtca tgggggagat
      661 aagcaccaaa tgttatattg atatacccaa gatagtaaga caggtcattt gtgagatagg
      721 atatacaaaa agcgaatacg gatttgacgg agacacatgt gcagtaatga catccataga
      781 tgaacagtct ggagatatag ccatgggagt ggatcccggc ggtgccgggg atcagggaat
      841 aatgttcggt tacgcatgca atgaaacaaa ggaacttatg cccatgccca tatctctttc
      901 tcataaactg gcaagacaac tggcaaaggt aaggaaaaac gggaaaatca agtatctgag
      961 acccgacggc aaaacccagg taacagtgga gtatgacggg gataaagtaa aaagaataga
     1021 tactgttctg atatccaccc agcatgattc ggatgtgaaa ctttcggcaa taaaaaggga
     1081 ccttgtggaa tttgtgataa agcctgtgat acccaaaaaa ctcatggaca aagacacaaa
     1141 gatactggtc aatcccaccg gaaggttcgt aataggcgga cctcacggag attccggtct
     1201 tacggggaga aagataatag ttgatactta cggtggatac ggacatcatg ggggagggtg
     1261 tttcagcggc aaggacccca cgaaagttga cagatcagcc acatatgcag ccagatatgt
     1321 ggcaaagaac atagttgcgg cagggcttgc aaacaaatgc gagatacagc tggcatatgc
     1381 catcggtgtg gcggagcccg tttctgtcat ggtcgatact ttcgggaccg gcagggtacc
     1441 cgatgaaaaa ctggctgaca tagtagtcaa gcatttcgat cttcgcccca aatcaataat
     1501 gagaaatctt gatctgagaa aacccatcta cagacaggtg gcggcatatg gccatttcgg
     1561 aagatccgat ctggatctgc cgtgggagag aacagacaaa gcggaagcac tgaagaaaga
     1621 attgaaaaaa tgaaagttat aaaattcgga ggatcgtcag cagctgatga tatccaaatc
     1681 gcaaaaataa aaaacatagt cgaaacagac aaaagcagaa ggtatgtagt cgtttccgct
     1741 ccgggcagaa ggtttgacca ggacagcaaa ataaccgaca tactttattc ctgcttttcc
     1801 agcagggaac agaacgtacc atatgaacag ctttttcagg tcatatgcga caggttctat
     1861 gccatggaaa tgaatcttgg gataaatgta aatatgaagg ctgttttggc ggatatcaag
     1921 gagaaatttc aaaatggggc atcactggat tatatggcca gcagagggga gtttttaagt
     1981 gggaaaatga tagccgaata tatcggatat gattttgttg acgcggcaga tatcattaaa
     2041 tttgacaaaa agggaagact tctggaagag aatacggaag aagccatcaa agacagtctc
     2101 aaaaaccacg aaagagcagt catacccgga ttttacggaa gcaagcccga cggcagcatc
     2161 aagacctttc caagaggagg ctcagacata accggagcgc ttgtggcaag tgccatgaag
     2221 gcagacgtct acgaaaactg gactgatgtt tcaggattta tggtggcgga tcccaggata
     2281 ataaaggacc cgaaaccaat aagcactatt tcatacatgg aattaagaga actttcatat
     2341 atgggagctt ctgttttgca tgaagaggcc atttatcccg cccgtgttgc gaacataccc
     2401 ataaacataa ggaacaccaa tgaaccggat aatcccggaa ccatgataac tgcagagccc
     2461 tgcgaagaag caaatgacag gacagttacc ggcatagcag gaagcaggga ttttgcggta
     2521 ataggtataa acaagaacca cctggccacc gaaaggggtt ttgtcaggaa actggcgggc
     2581 atactggagg ataacgatgt tcccctggaa catatgccca gcggcattga tactctttct
     2641 gtggttgttt caaagaaaaa tgtcaatgga aaactggatg acataatgga cgaatttgaa
     2701 cagcagctga aacccgatac catggagata tttgaggaca tggcacttat cgccactgtg
     2761 ggatgcggca tgtcatttac acccggtgtt gcggccaggc tgtttacggc ccttgccgat
     2821 gccggagtga acataagaat gatcgatcag ggttccagcg aaatgaatat aatcgtagga
     2881 gtaagcaata aagattttga aaaatccata aaagcgatat acaaggcatt tattgagtaa
     2941 aaggagaaaa ggagaatgag gctgagaaaa agaatactgt catgggtagt agttttggct
     3001 ctggctgttt ctgtgatcgc aatagacaca gacaaagcgg atgctgccag ttcacgtaca
     3061 tactggatca aagtcaacaa acaggcaaat gtggcaactg tatacaaaag atccggaagc
     3121 aagtggaaac ccataagggc gatgctggtg accaccggta caaatgcctg gtatgccaag
     3181 gaaaagatgt ggtgtatcac tcccggcggc aatttccgga tagggatcaa aatgcgttgg
     3241 ggagaaatga tggaagaagt atatgcccag tactgttctc aaataagggg cagtatactt
     3301 tttcattcgg ttttttataa caaaaaaaac agtccgtctt cccaggccag cggacagttc
     3361 tacagactgg ggagtaaagg ttcccacggc tgcgtcagac tttgtgtcat ggatgccaaa
     3421 tggatatacg aaaattgccc cagagggacc aaagtcacag tttacagcag caaaaaagcg
     3481 ggacccctgg gcaaacccaa aagagtgggc gtaaaggtct acggatggga tcccacagat
     3541 cctaatccaa aaaatcccgg gttcaacatg agaagaccgg taattaaaat atcaaaaaag
     3601 aagcctgcga ccgtacagtt tggaaattca tacagtctga agtccaaggt gacggccaaa
     3661 aatacgaatg caaaccagag ccttacttcc aagctgaagg tttcttcagt caagaagtgg
     3721 aagagcggca aatggaaaaa agtaaaaaaa ttcaccacca gatccctggg gaaatataag
     3781 ataacatata aagtacggga taaatactgc gggaaaaggg gaacggcgaa aaaatctttt
     3841 gttgtaaagg tggtggataa aaaggcacct gtgatctatg cctataacag aacaacaaag
     3901 ccgggactca gcaccgctgt aacaggagta tccgcaaaac aggcagacgg cacaagcaga
     3961 acagcagcaa tgaccgttac agtcacagga cctcagggca gcagaaccat gtcatatgcc
     4021 caggcaagag gatatgtact tagcagggaa ggcgtatata ccataactta ctccgtcagg
     4081 aatacttaca agccatataa aaaggcagtc agaacaattg cagttcgctg ttacggagat
     4141 gcggtaatga gcttaacaga tcctctgcct gaaacaatca ctgttgatcc cgacagcggc
     4201 atgatcatga tcccggcaca ggaacaggcg gtggaaccgg agaccgttga ttatattacc
     4261 gcagacaaac tcaaagaaga aatcggaaca ataatcagaa tccgggatgt aaatgccgcc
     4321 ggaacaagaa gcgataagat gctgaatact gttctgaatt acaaaaactt tatttcactg
     4381 tccccgatac tcaatgaggg agaaaattat ataacaggag acgaagtgac ggcaacaatc
     4441 acatatagag gttttaacgg aacaacaata tcaaaaccaa taactatcat aatagttgag
     4501 gccccggatc cggatccgtt gccggaaaaa taatatcgat ctacataaag aaacaagaaa
     4561 atacagatga cccggtttct tgccgggcca tctgtattgg ataatggggc attagctcag
     4621 ccgggagagc gcgtggttcg caatcacgag gccaggggtt cgatccccct atgctccacc
     4681 atcgatgata aagcacctct gcataatttt actgcggggg tcttttttta tgtttttaat
     4741 aaacaaaaag tatttcaaaa accgggagag aggcatagcc ctgatcacca cgcttaaagg
     4801 caaacaaaaa aagatatggc agaaagaaaa gatcattatt gccggacaaa aacaggatat
     4861 atcgaatcaa tgtaagcgga cagaaaattt ttctgcccgc tttttttgtt gattataggt
     4921 gtatctgcga gtataatatc attaaatggg gtgctgtttg aaaaaaacag tatgcaggga
     4981 agttaccttt ctttagaaaa ataaaaccca aaaaagattt tcacaccaat gtcacaataa
     5041 gtgaccgaaa gggtgggtca ttatgctcct taatacagag acaacagaaa aatcagggga
     5101 tgatccaaat ataaaaagac gcaagcagat acatgcaata gtgcttctta ttgtaatact
     5161 tatgtgtgca gtttatttga gttttgccta taacagacac agcaatacga tgtcttctga
     5221 agccataatg ctgggagagt ccctttgctc tcttcttgat actgaacaca ttaaagaact
     5281 ttccggagat ccgggagata caagcagcat agactataaa agtacaaagc agagccttat
     5341 cagagtgata caggaagata actggttgca ttttgcctat ataatgtcag taaaagacgg
     5401 caaaatgcta tttttattgg attcagagcc tcctgattct cccgattatt ctcctcccgg
     5461 acaaatatat gaagaagcca ccgatgaaga ctgggaacct ttccgtggag aaaagaccgt
     5521 gctgacaaaa cctgtatcgg acagatgggg gacatggata agtactctgg tacccattga
     5581 agatgaaaag accggcaagg taatcgccag tttcggtatc gattatgatg cattagaatg
     5641 gaatgttcgc ctgtggaaaa agatgatacc ggatatcatc attgttattt gtttttttgc
     5701 tctttcaatg gttctgatgc acacatggaa tcagcaggaa gtattaagaa agcttaccag
     5761 aagacttgca gttgacgagg ctctctataa aggccttttt gaacagatgc ccataggagt
     5821 gtcagtaatg aacgacacaa actggtattt cggcagtgaa ttcaaaaaaa taaatattaa
     5881 ccaaaagttc gaggatataa tgggaaggac aagagatcag cttaaaaacc ttaattggat
     5941 ggacataact taccccgaag atctggcgga agatgttgag aagtttaggc aattcaagag
     6001 tggcgaaatc aaggggtaca gcatggagaa acgatttatt aaaccggatg gaagtgtgat
     6061 ttgggtaagg atgacgattt ctcctttcat tttcagcgaa gacaaaaggc cattgcattt
     6121 gtgccttatc gaggacataa cagaacgtaa ggaaatcgca gaaacactca aagaaactga
     6181 acggaacaaa gcagtacttt tttcaaatct tccgggaatg gctttcatat gcaaatatga
     6241 caggaactgg acaatggaat tcgtatccga tggatgctat ggccttacag ggtatgaatc
     6301 cgatagtctt gtcaacaaca gtaaaattgc ctttaatgag atcgtgatgc ccgaatacag
     6361 agatatcctg tggcaggaag cagaaaaaac attagggaac aaaatgccgc ttaaatatga
     6421 atatgagata ataacagccg aaggagaggt aaaatgggtc ctgatgatgg cacaggccgt
     6481 atatgacgaa aaagagaaaa taaaatcagt tgaaggaata atacttgata taaccgacag
     6541 aaagaaactg gaacaaaggc ttgagtacca gagtatgcat gatgaagcaa caggacttca
     6601 caaccgcatt tatcttgagg aagtgttaag aaaagaaata aagaaggacg agaaagagta
     6661 cagagctttg atatgcttaa acttaagcac tatgaacaat ctgactctca cctatggatt
     6721 cgaatacaag tatcagctta tgaagaggat aaccgggtcc ctgaaagtgt tttgtgacaa
     6781 atatcacagt ttgtttatta cagatgagac ccatcttgtt ttctatgtaa gaggctataa
     6841 aaccggaaaa gaagtattag cactttgcca gaacatttcg gagaaactgg agagagtcct
     6901 tgaaatcgaa aggctcggag cgggcatagg tgtgatggaa atagacaaag acaaccggga
     6961 tgatatcgat acgatcctca aggatcttat gactgtgtca gagaaagctc tgaatacaat
     7021 gaataaagaa atagggatat catttttcga tgatgaaatg aaacagcagg tattccgtga
     7081 tgaagagata agcagagagc tttctcagat cgccgccggt gaaagaactg aaaggctgtt
     7141 tcttcagttc cagcccataa tggatcttga ttcaaaaaag atatgctgtt tcgaagcact
     7201 ggccagaatg aaaagcgata atcccaaact cattccccca ctggagttca taccaatagc
     7261 agaaaaaacc aagctgatag tacccctggg ggatcatata atacaaaaat cccttgagtt
     7321 tatgaaaaag ctggaggcaa acggccacaa ggacataagt gtatctatca atatttccgc
     7381 aatacagtta ttaagagaag gctttgcgga aaacctgatt tctgttgtgg atgaaatggg
     7441 cataactcat gggaacgtgg cgctggagtt cacggaatca atgttttctt cggatttcga
     7501 ttatataaac gaggttttga aaaaactgag gtcatatgga ataaaaacgg caatagatga
     7561 tttcggcaca ggctattcat cctttgcaag ggaacgggaa ctcaatattg attatctgaa
     7621 aatcgacaaa ttctttatag ataagataat ggtacttgaa tccaaggaaa ccataacagg
     7681 ggatataata tcaatggcgc acaagctggg acacggcgtt gtagccgaag gtgtggaata
     7741 tgagatgcag tttgaatatc tgaaggaaca tgggtgtgac aagatacagg gatatcttgt
     7801 aagcaggccc cttgatgaaa acgtggctat cgaacttctg aaagaacaaa aaaatatggg
     7861 atgggaatag tgccttcaga ccggagaaaa gggggaaaag gaggtggaaa gaaatggaca
     7921 aaaaaatata tgaagaagcc atacaaaaaa gcattgtcgc atttgcttat cataaaggag
     7981 ttttcgatga cagcgggaga atgatagatt atatttttat tgacatgaac cctgtatttg
     8041 aaaaatatac tggactgaaa aaagaagata taatcggcaa aagatttgcg gatgagatcg
     8101 cgctggacaa agagcatgca gcagaatggg tacaaagata tgcaaaggtc attgctgaac
     8161 aaaaggaaga tcgttttgaa gagtatgcaa aagaatacag aagaaatttt gatataatcg
     8221 cttaccccac agatgagaaa cactttgtta ctctctttgt gaacagaacc tttgagaaaa
     8281 aagttcagga aatagcaagg tttttcatag acaatatggg aggacatata gattatgaca
     8341 aactaacaga atttgcgtat gaggtatcgg gagcgcagta tgttgcattc aaccttttcg
     8401 ataaaaacgg aaaggatttt aaaacagtat ccatatatgg agagacagaa attttcaata
     8461 aaaatctgga actcatcaaa aggcatcttc ttaaaagaaa atgggctcat gatcctgtga
     8521 aagaacaaaa gaccggcaga aacgatataa cggtttttga aaatctgcat gatcttacgg
     8581 gagaagtaat tcctcaggaa acagtgatgc aaatcgagag agactatgat ataggcgatg
     8641 tggtggtggc aaaaataaca aaagaaagaa aagttatcgg tgattttact ctgtttttca
     8701 aaaaaggcaa aagccgtatc aatcatgatc ttttcaaact ttttctgctg cagacaggac
     8761 tttttatcga aaaaacaaga ctggaaagat ctcttttcag gagccagaaa agatttttca
     8821 ccctggcaga atatgcacct atcggattca tggcctgcga caggaaaggc aatataactt
     8881 atgcaaataa aaaactactt gaaataatgg attcgccttc ctacgaagcc acaaaatcca
     8941 tcaatttaat tgaatttgtt cctcttaaga aagaagggtt ttctgacaaa ctcgtagaat
     9001 gcatggaaaa gaatcgtgaa attacatatg aaatgggata tagaagtctg tggggcaaaa
     9061 aaacctggct aagagtttac tacaaaccgt tcaaaacaga agaagaagta agcggcgctc
     9121 atatggttgt tgacgatata acggagagga agaaagaaga agatatttta aaaaatatgg
     9181 ctgaaagaga tgctctgaca aaaacattca accgtcacgc ccttgaaaca gttcttatag
     9241 acagattgaa cgaggccgaa gataatgatc ttgtaagttg catctgtatc attgatatag
     9301 atggattcaa aatgataaat gatacctatg gtcatgatgt gggagacagc gtacttaaat
     9361 atcttgcttc cagagcaaaa aaagaactca gagaaaaaga tcttcttata aggaccggag
     9421 gagacgaatt ccttatttat tttcacgata taaaaagcga agaaaacaca gaggatcttg
     9481 taaaaaggat attcgataaa atatccacca attacaggtt tcaagatcta aatgaagaaa
     9541 ataaactttc ccttgaagtc ggatgcagca taggagtctc tctatatccg aggcatggca
     9601 gaacaattaa agaactgatg gcaaaagcag ataccgctct ttatgaagtc aaaaagagcg
     9661 gcaaatcaaa ttatcatatt attgattgag atatccctga ggatctcagt ctttccaaaa
     9721 cccgtgtgtt atatagtctt caatgaattt tgatgcaacg gcaccgtctg atgtggctgt
     9781 aacaacctga cgcagtggtt tttccctgag atctccgaca gcaaaaactc cgggttcgct
     9841 ggttcttgtg gtttcgtctg cgactatata cccttccggg ttcatttcta tttttttatg
     9901 aaaaatctca gtgttgggca tcctgcctat ggctatgaaa acaccgtcac aggctatttc
     9961 agaaacagag ccgtccttca ggttttcaac ttttacgcct gtaactttat tgtcatgcat
    10021 tacttctttg atctttgaat cccagacaaa agtaagattt gaagcatttt ccaacggagc
    10081 catataagtg ggggaagctt tcaaggtgtc atgcctgtga accaggtaaa cttttttgca
    10141 tatcttagaa agtatgagtg cgtcagctgc agccgaattg ccgcccccta cgatcacgac
    10201 cttcttttcc ttgtaaaaca ttccgtcaca tgtggcacag tatgatatgc cgttgccggt
    10261 aagttgtttt tcgtagggca gtcccagatt tctgggagcc gcaccggttg cgatgactat
    10321 ggtctttgtg tgcagcactt taccgtcggc cagttttatg gttttgtccg gacctttcat
    10381 gtccacttct gtaacttctg cgaaaattgt ttcggcaccg aaacggtctg cacccgattt
    10441 cattttgccg gccagttcaa ctccgtctat ccccatgtca aatccgggat aattgtctat
    10501 catgctggtt gtagccattt gtccgccggg ataaagcttt tctattatca gtgttttgag
    10561 gttcgctctg gcacagtata aagcagcaga atatccgccg ggacctccgc cgattattat
    10621 tgtatcgtac ttattatgat ccattttcta cctcctatta tgacagggct gcgtttcatg
    10681 atttatttac aaataattat acccttttta taaagcataa aacaggacaa aaaagcgaaa
    10741 cttcataaac aaagatatcc tcctgcgttt tttcatttgc cataggagga tatcttaata
    10801 aaatttaatt gtatttcata gcttcgtttg caatgtactg ttttttgcgt caatcattac
    10861 ccatagttgt cacctccctg ttatatttca aaagatttaa gctctcagga cttttcagta
    10921 tcaggaatga aatagtcacg cacctgcata aggaagaaca gtgcaattat ggtgcctata
    10981 ataacaccta tcaagccacc tatgcttccc atgaagatgt cattaagtac agaaaaaatc
    11041 agctgtatcc aaataacata aaatgccagg cgccaaccca gatagctttt gtttttcaaa
    11101 ggtttgatgg ctattatctc aagaactact acgatagcca ttcctataac accgaatatg
    11161 actattcccc atattgccat tgaatggaag cccaaaccaa atccggccaa cactgaaaaa
    11221 ccgaaggatc caactcccaa aagggttatt attccaataa tgttaaaaac aatgcttata
    11281 atgatcagga tccacaaaat gttcgcaata acacttttgc cattttcggg gatctgcggc
    11341 agggtcttgt tgaactctgc cagcttgttt tccaggaatt tcattttttt cacctctttt
    11401 cattgatcta aaaatacaca ctaaataata tcatatatat aactttatca aatcatgggt
    11461 ttttacgcaa gagataaagg agaaagtgtc ttaaaacaat gaaaaaaagt aaaaaagtca
    11521 agaagtactt ttattgagca atacgcaaaa cgatcaataa ttatttacat attcatttat
    11581 aaatttgacc gctgcatttt catccagcgg tttgctgata agaaatccct ggactttgtc
    11641 acaaccatgc tcttttaagt attggatctg tttgatattt tcaacacctt cggctacaac
    11701 gtagtgtcct tgcttgtgag ccatttcaat aatatgaccg gttatgggtc tgtcggcagc
    11761 cagtatatga tcgataaaaa atttatcgat ttttatgtag tttacgttca aattcatttc
    11821 ccgtgcaaga gaggagtatc ccgaaccgaa gtcgtctatg gccagagcaa gaccgctttt
    11881 tttgagttca acgagtacac tatttataaa atcaaagtca tattcgaaaa tggactctgt
    11941 aatttcaatg acgacatttt tcggatttat tttcttttta tgaataattt caatcagcat
    12001 ttctgcgaag cccggtgtta aaagctgaat aacggatatg ttgatcgcaa taattatccc
    12061 ttcaaatccc ttttcttcaa gttttttctg gaagtcaagg gcacggagaa tgattttttc
    12121 tccgatggga ataatcagtt tgcttttttc agctatggga ataaattcag caggtgaaac
    12181 tgatcccagt tcatcagttt ttaatctggc taaagcctca aaagcgcaga tagattctgt
    12241 ttccaagtct aaaattggtt gatactgtaa aaacagttca ccgccctgat catttaccgc
    12301 tatcgtggat aaggcttttc ttatttcttt ttcacggttt ttttcaagtt ccagttcttt
    12361 gttataaaaa caggctttga agtccttatc gaatatacca attgagtttt cagtagcaat
    12421 aatcagtttt ctgcgaatca tatcaatatc aaagttattt tggtcttgct caatttcaac
    12481 aacgccgact cctccggaca ctctgtcctg aataaacaaa gttcttaaca atcccaatat
    12541 agtctcagtg aaatcaatca actcgttttt gtccttgtaa tctctcaggt aaaaaacaaa
    12601 acgcccttca ctggttttga acaataaacg tttgtcagtg caatattcgc tcagtgcaaa
    12661 agcaacattt tttatcatat cttgcgtgta ctgataaccg gaatatgccg ttagcagata
    12721 aaatgtattc aaattgatac ttatagcggc tctctttcgt tcgtactttt tcttgctgtc
    12781 tttttcaaga agcaaatcta aatagtaact attatataaa cctgtcaaag catcatgctc
    12841 actaatatat ttcagatgat cttctatatt ttttctgtca gtgatgtcta gtatgattcc
    12901 ctcaagtgca ataacatcac ctttttcatc atatacacat tcgcccattt caagtaccca
    12961 tttgcgtttt gaatcgattg tagtgatttc atattcgaaa tttaattttt ccttgttttc
    13021 tgcggcctgc ttacgattgg tccaaacgct ttccttgtat tcaggtgcta taatatcatt
    13081 aaaggtcaca tcacccgaat cataaagtat atcgggggca tatcctgtca gttccaggca
    13141 accgtctgaa acaaact
//
LOCUS       DAONHK010000018        13007 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_3760_length_13007_cov_4.389747, whole genome shotgun sequence.
ACCESSION   DAONHK010000018 DAONHK010000000
VERSION     DAONHK010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 13007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13007
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3760_length_13007_cov_4.389747"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(113..189)
                     /locus_tag="PLM92_01340"
     tRNA            complement(113..189)
                     /locus_tag="PLM92_01340"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(154..156),aa:Thr,seq:ggt)
     gene            complement(287..931)
                     /gene="sigH"
                     /locus_tag="PLM92_01345"
     CDS             complement(287..931)
                     /gene="sigH"
                     /locus_tag="PLM92_01345"
                     /inference="COORDINATES: protein motif:HMM:NF006148.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigH"
                     /protein_id="HPR23818.1"
                     /translation="MEKNNRKKEYAKKREVTKFGDLSDEQIAVLAQEDDQEALEFLIR
                     KYMSIVKKKKNTYFIMGAESDDVVQEGTIGLFKAIKSYDKEREASFSTFAEICVNRQI
                     LSAIKAASRMKHFPLNNSLSLSSPAEETEKTLEDVISSRKITDPETLIILKDVFGYIS
                     KNEGKLFSELEKTVWSEYVKGKSYVEISQKLGKSQKAVYNAMERMKKKIILYLG"
     gene            complement(918..1705)
                     /gene="rlmB"
                     /locus_tag="PLM92_01350"
                     /pseudo
     CDS             complement(918..1705)
                     /gene="rlmB"
                     /locus_tag="PLM92_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282452.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
     gene            complement(1692..2168)
                     /locus_tag="PLM92_01355"
     CDS             complement(1692..2168)
                     /locus_tag="PLM92_01355"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HPR23819.1"
                     /translation="MVADEKWGIGKDGKLLAHLPGDLKYFKEKTKGKTLVMGRKTLES
                     LPGHKPLPDRKNIILTSDDCFEKDGCVVCNGMDQFFDEIFFDDGQDVFVAGGESIYEQ
                     FLPYCEEIYVTRIMETFDADRYFIDLDESEDFIKTWSSDICEDKGVRYRFEKYERK"
     gene            complement(2192..3022)
                     /gene="thyA"
                     /locus_tag="PLM92_01360"
     CDS             complement(2192..3022)
                     /gene="thyA"
                     /locus_tag="PLM92_01360"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HPR23820.1"
                     /translation="MSRADRLFIDMCEDILQNGFSTEGQTVRARWEDGAPAHTIKKFG
                     VINRYDLSKEFPALTLRPTAIKSAVDELLWIWQKKSNNINDLNSHIWDQWADKEGSIG
                     KAYGYQLGVKYKFAQGEMDQVDNVIWQLKNQPYSRRIMTNIFNFRDLLEMGLEPCAYS
                     MTFNVTGDRLNAILNQRSQDILAANNWNVVQYSVLVHMLAQVTGFRAGELVHVISDAH
                     IYDRHIPIIKELIKRPVYPAPKFKLDPDIKDFYQFKTEHVIIEDYEKNPQIKGIPIAV
                     "
     gene            complement(3034..3243)
                     /locus_tag="PLM92_01365"
     CDS             complement(3034..3243)
                     /locus_tag="PLM92_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPR23821.1"
                     /translation="MSKKTRNKFRDMVGDYYMTNREVDKENRSRRKEERKAGGEEKFW
                     TPTEKILLAVIITGAIGIIVKYLVF"
     gene            complement(3221..3616)
                     /locus_tag="PLM92_01370"
     CDS             complement(3221..3616)
                     /locus_tag="PLM92_01370"
                     /inference="COORDINATES: protein motif:HMM:NF012843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III domain-containing protein"
                     /protein_id="HPR23822.1"
                     /translation="MTEDLLKRDTTALAFVGDAVQELYIRKMLFEKGPQKTSRMHKNA
                     VMHVRADGQAKAIKDIFEDLTEDEKVLVKRARNKRSTSRPQNADPVAYKWATAFEALI
                     GYIYLTGDSKRLEEILGKAVGCIVKENQK"
     gene            complement(3624..5027)
                     /gene="cysS"
                     /locus_tag="PLM92_01375"
     CDS             complement(3624..5027)
                     /gene="cysS"
                     /locus_tag="PLM92_01375"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005998641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HPR23823.1"
                     /translation="MKIYNTLTRSKEEFIPADSSEIKIYVCGPTVYNYFHIGNARPFV
                     VFDTLRKYLQYRGEKVRFVQNFTDVDDKIINKAKEEGVSASEISEKYIEEYYKDAEAL
                     NVAKADFHPKVTENIDEIVKFVKDLIDKGYAYEVDGDVYFSARKFKDYGKLSKQNIED
                     LESGARIMVEEKKKDPLDFALWKARKKDDEIAWESPWGMGRPGWHIECSTMSKKYLGD
                     TIDLHAGGQDLTFPHHENEVAQSEAHNGKPFAKYWMHNGYITIDNEKMSKSRGNFFTV
                     RDILRSYSGEVIRYFLLSGHYRNPINFSDELMEQAKNSLERIRNASLNLKHLIKNGSG
                     TMTEEEKKKIEDMDKYRQMFISAMDDDLNTADAITAVFELITEINTAVKQNVSREFAE
                     KCLETLMEFCNVLGFLQDGQEEDGAVDPEIDKLIEERRLARQKKDFARADEIRDMLKA
                     KGITLKDTPQGVQIIKE"
     gene            complement(4996..5661)
                     /gene="cysE"
                     /locus_tag="PLM92_01380"
     CDS             complement(4996..5661)
                     /gene="cysE"
                     /locus_tag="PLM92_01380"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005838496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HPR23824.1"
                     /translation="MAFFKTTREDIKTVLEKDPAARNGFEVFLCCPGIWARMWHIPAH
                     FLYKHNIKLLARIISQLTRFFTGIEIHPGAIIGRRCFIDHGMSVVIGETTEIGDDVSI
                     YQGVTLGGTGKDTGKRHPTIGDRVIVSSGAKVLGPFKVGNDVKIGAGSVVLKEVPDGC
                     TVVGVPGIIIKRPGQHTEEMNQVDLPDPTTVEMECLRRRIVMLEQKVKELENENIQHS
                     DEK"
     gene            complement(5738..6766)
                     /locus_tag="PLM92_01385"
     CDS             complement(5738..6766)
                     /locus_tag="PLM92_01385"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HPR23825.1"
                     /translation="MDLIKKHHKKKISAAALIVFVFSIALGLLMTGEPIDKPLITATG
                     QVMQHPWVIKVEDKKAAILSSEEAAMLAIEGIKKQYINEENKILFLKFKEEVKIEKLD
                     ADNGCNAPELFTIDEAIKELTEGRNSLTVISGELCEDIKITKCKTIRKSVTSLEAGTE
                     KIKKNGSSGKTEIKTKIIKENGKVVSKEIIEKNVLSKASSRVILVGTAGADLKNTGSG
                     EFGRPLSSMNVISGFGPRGGAFHDGIDIGAGYGTAVYASQSGYVLQAGESGYGWGIIV
                     KVRHKNGMTTIYAHCQSVNVRAGQSVKRGQKIAEVGSSGNASSPHLHFEIWRGSSLST
                     AVDPMKYL"
     gene            complement(6879..8177)
                     /locus_tag="PLM92_01390"
     CDS             complement(6879..8177)
                     /locus_tag="PLM92_01390"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HPR23826.1"
                     /translation="MLKEKAIKAGSSLVITVKRLLTGAGDRIRIKDLGRVKTLLAPTE
                     IIKNLKNRVLDIRRAGIKGTAVGIKNGLRSFLNETDIFHLTLPQKKKLGVFAAVIFVF
                     SLTVGWASTSQAIDRPFVSAEGEVMETPWVISVEGEDKIVARSRDAAQLIMDGVKQNY
                     VCEKNQIIDVKIKEDIKICEMDIKNGQQEPDICDIDEGISKITEENAVTVAAKEICTA
                     KKETKYKTIYKKTDSLPAGTKKVKTEGAKGEKEVVTELIKENGKVIEKEVLDEQTTKK
                     AKAKVVLVGTAASSSSSSSYGSSYSSSGGSIDNHSGTLGRPLSSMRHLSSFGMRWGRM
                     HYGIDLGGSMGTPIYASESGKVTQACYSGTYGLIVKISHGGGLETRYAHCSRVHVKVG
                     QRVSRGQKIASMGATGNARGTHLHYEVRVNGVARNPYNYL"
     gene            complement(8288..9088)
                     /locus_tag="PLM92_01395"
     CDS             complement(8288..9088)
                     /locus_tag="PLM92_01395"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HPR23827.1"
                     /translation="MLTGEKTDMHLHTWYSDGKMSPEEVVRYAKNKGLNKISVTDHDG
                     VSGIQEALEAGRKYNIEVIPGIELSAAADGKGVHILGYFIDYENKELNRACHRARRWR
                     KERNKKLLDLLRKKGFDLEEKDLVFRKGQDYLGKPQIARAMVNKGYIKCEKDAFEPGG
                     IFDQKDIRSIKKEKITAALAISLINGAGGKAVLAHPAKIRHIGERESSEFEENLEKLL
                     RKMKDMGLYGLECLYTEHSQKENRIFLQMADEFGLVPTKGSDFHGNNT"
     gene            complement(9090..9896)
                     /locus_tag="PLM92_01400"
     CDS             complement(9090..9896)
                     /locus_tag="PLM92_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPR23828.1"
                     /translation="MANMFKRTKRDPRIVPKKKTLASRIEGLKRSFRRNSRKNPWKQN
                     KKRKSVPWAKIMAPLLIVMIPVAVMYAGDNILLRTTALYSFHLNSTEILSEEMVYAEN
                     EDVVGLFGDYMMGKTDEFRMKENLEYMPEELFNEIDALTVKNIRYNLDKEAYAAGVIL
                     LLVIGIYIYLYRKKEKDLLLRNYFYSIVGFAVLAIFDTLTVAVKPLRQAIHGDLSSFN
                     FPKDDLLVKILDTSFMWRYSLFSILVAAIMMGIGLYITLAVAGRHHMFRR"
     gene            complement(9935..10861)
                     /locus_tag="PLM92_01405"
     CDS             complement(9935..10861)
                     /locus_tag="PLM92_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPR23829.1"
                     /translation="MESFLAAAIVAAMPLLYATIGEIMTEKVGNLNLGVEGMMLMGAV
                     IGFIAGHTTGSPQIAVLAACVAGASGAAIFAVLTVSLRTNQVVTGLTLTIFGTGFANL
                     VGRSYVGVPLTPVVRDAFDSIAIPGLSQIPIIGRALFDQDILVYMGFILVAVTAFYYN
                     KTRIGLNTRAVGENAAAADASGINISLYKYANIIIGGALCGLGGAYMSLGTIPVWQEN
                     VVAGRGWIAVALVIFASWKSIKGFLGALLFGALSIIGFRLQAMGISVSQYLVDMIPYL
                     ATIIIVIISTHKNRKEDLPPGDLSNPYFREER"
     gene            complement(10863..11939)
                     /locus_tag="PLM92_01410"
     CDS             complement(10863..11939)
                     /locus_tag="PLM92_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPR23830.1"
                     /translation="MIKITKKAALPKKQEAVIQVIAILLSLACAGIVILAMGYDPLEA
                     YYRIVEGSIGSVMRIEQTITKSIPLIIVSMGIVVAFKMKFWNIGGEGQIMMGAFGATL
                     VALNFPDTLPAPVMILCMMAASILCGAIWAFIPAFFKARFGTNETIFTLMLNYIAIKF
                     VTYLQFGPWKDPNSGGFPKIETFSQNAQLPDFLGIHSGWVVAILSVAFLYVYINYTKK
                     GYEISVVGESLETARYAGMNIKGIIISAMLVSGGLCGLAGMIQVSAVERTLTYSISAG
                     YGFTAIITAWLGRLSPPIVMIVCILFAMLLQGGAYIQVAMSIPSSVADMIQGVILFFV
                     LGSEFFMQYKVSFKKRPGSKKEVA"
     gene            complement(11923..>13007)
                     /locus_tag="PLM92_01415"
     CDS             complement(11923..>13007)
                     /locus_tag="PLM92_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012198795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPR23831.1"
                     /translation="IKVLYRGAHILILDEPTAVLTPQEIEKLFHIIRNMRDQGCAVVI
                     ITHKLNEVMEISDRVTVLRKGRSVATVETADVDVGTLIEMMVGKKVDLAIKKEKGQEA
                     GVLLTMDGVEVQDKDGKNALENISFELEGGEILGVAGVAKSGQKELCECIAGLIRTNK
                     GRIFFQGENIVGKTPADIIKLGIRMGFIPEDRLGMGLVGSMDIVHNIVLKDYQGQPGI
                     FLKRGPCARKAEKIVKALDIMTPDIYTPVKKLSGGNIQKVLLGREIDSDPKVLITAYP
                     VRGLDIGASYKIYDLLNEQKQKGVGVLFIGEDLDVLMSLCDRIMVLCQGEITGIVDPD
                     EVTKEDIGLLMTGGSLEEEAKHDKNN"
BASE COUNT         3038 a         3277 c         2338 g         4354 t
ORIGIN      
        1 tttatgtgcg gcttattcct ttcaaatttc tcctttgcca ttttttcttc ctccttggca
       61 ccttcctact gccggttatt ttggccggtc aacaaaaaat cattatctat gttggagctg
      121 ttgagcagga ttgaactgcc gaacctcttc cttaccaagg aagtgctcta ccaactgagc
      181 tacaacagcg catcaacaat gcgtatttta ctatattttc aacaggctgt caacgggaac
      241 gatgcttatc tcacgttgcg ctgccgtcac caagagtctt ttgatgctat cccagataaa
      301 ggattatttt cttcttcatc ctttccattg cattatacac ggctttctgg cttttgccca
      361 gtttttgtga tatttctacg taactcttgc cttttacata ttcgctccag actgttttct
      421 ccagttcact gaaaagtttg ccttcatttt ttgaaatata cccaaacaca tctttaagta
      481 taataagcgt ctcgggatcc gtgatcttgc gggatgagat aacatcttcc agggtctttt
      541 ctgtttcttc cgccggactg ctcaaagaaa gggagttgtt aaggggaaaa tgtttcatgc
      601 ggcttgcagc ttttattgcg gaaaggatct gccggttcac acatatctct gcaaaggtgc
      661 tgaaagaagc ttctctttct ttgtcgtaac tttttattgc cttgaaaagt cctatagtgc
      721 cttcctgcac cacatcatcg ctttccgctc ccataataaa atatgtgttc ttctttttct
      781 tcactatgct catatatttt ctgatgagga actcaagagc ttcctgatcg tcttcctggg
      841 caagtacggc tatctgctca tcggaaagat caccaaactt cgtgacttct cttttttttg
      901 catattcttt tttccgatta tttttttcca tttctctgcc ttctgatttc atacatgagg
      961 actgcagctg catttgatgc attaagtgat tttatttttc caaccgccgg tatcgatacg
     1021 gcaaaatcac acttttcttt tattaatctt cctatcccgc ttccctctcc tccgataaca
     1081 aggccgacag gtcccgtaag atcgggaagt gcttcttctc catccggata tatgcttttg
     1141 ccgtccatgt cacatgcaac gatccataat cctctttttt ttaaatcttc caaagtcctg
     1201 ctcagatttg tcacttgggt gcacatcata tgctccgatg cgccggcgga aactttcgcc
     1261 gccgtctctg tcaccccgac agatcttctt tttggtatta ctataccgtg agccccggca
     1321 cattcagctg tccttattat ggctcccaga ttatgtggat cttctatgcc gtccagcagc
     1381 aaaagaaagg ggtcctctcc tttttgctcc gccagcagca tcatgtcgtc aatgctgcta
     1441 tatttgtatt ctgaaacata tgctattatg ccctggtgat ttttccgcct gatattttat
     1501 caagcactgc tctttcttca taccttacag ggatatttct ttctcttgca gaagcgatta
     1561 tttttttcac tgatccttcc gatctctttg cagctattat aaaagctgtt tctcttcccg
     1621 ccgataaggc ttctgaaaca gcgttcctgc cgataagtat attctctttt gccacgcttc
     1681 ccgcatgcct gttattttct ttcatatttc tcaaatctgt atctgactcc tttgtcttca
     1741 cagatgtcac tactccatgt ttttataaaa tcctctgact catccagatc aatgaagtat
     1801 ctgtcagcat caaaggtttc cattatacgg gtaacgtata tctcttcgca atatggaagg
     1861 aactgctcat atatagattc tcctccggcc acgaaaacat cctgtccgtc atcaaagaat
     1921 atctcgtcaa aaaactgatc cataccgttg catacaacac atccgtcttt ctcaaaacaa
     1981 tcatcactgg tcaggattat atttttccgg tcaggaagag gcttgtgacc cggcaatgat
     2041 tccagagttt ttctgcccat cacaagagtt ttaccctttg ttttttcttt aaaatatttc
     2101 agatcgccgg gcagatgagc cagaagtttg ccgtctttgc ctatacccca cttttcatct
     2161 gcaaccacta ttgctttgat tgtaagactc cttatactgc tattggtatc ccctttatct
     2221 gaggattctt ttcataatct tcaattatta catgttccgt tttgaactgg tagaaatctt
     2281 ttatatcggg atccagtttg aattttggtg caggatatac aggtcttttt atcagttctt
     2341 ttataatagg tatatgcctg tcataaatat gtgcatccga tatcacatgc accagttccc
     2401 cggctctgaa tccggtgacc tgggcgagca tatgtaccag cacggaatac tggacaacat
     2461 tccagttgtt tgccgcaagg atatcctggg aacgctggtt caatattgca ttcagtctgt
     2521 cgcctgtcac attgaaggtc atggaatatg cacatggttc cagtcccatt tccagcagat
     2581 cgcggaaatt gaatatattt gtcattatac gtctggaata gggctggttc ttaagctgcc
     2641 atatcacatt gtccacctga tccatttctc cctgggcaaa tttatatttg actcccagct
     2701 gatagccgta tgcctttcct atggatcctt ccttgtcggc ccactgatcc catatatgtg
     2761 aattaagatc atttatatta ttggatttct tttgccatat ccaaagaagc tcatccaccg
     2821 cagacttgat tgccgtaggt cttagggtca atgcgggaaa ttcttttgac agatcatacc
     2881 tgtttatgac accgaatttt tttatcgtat gggccggcgc gccgtcttcc catctggccc
     2941 ttaccgtttg cccttctgtg gaaaatccgt tttggagtat atcttcgcac atgtcaataa
     3001 acaatctgtc tgccctgctc atatctcttc ctcctaaaaa actaaatatt ttacaattat
     3061 ccctatggct cctgttataa ttactgccag aaggattttt tccgttggag tccagaattt
     3121 ttcttcgccg cctgcttttc tttcttcctt ccggcggctt ctgttttctt tatcaacttc
     3181 ccggttggtc atatagtaat cgcccaccat gtcccggaat ttatttctgg ttttctttga
     3241 caatacagcc gaccgccttt cctaatattt cctccagcct ttttgaatct cccgtaagat
     3301 aaatatatcc tataagagct tcaaatgccg tggcccattt atatgccacc gggtcggcat
     3361 tttgaggtct gctggtgctt cgcttgttgc gggccctttt gaccagtacc ttctcatctt
     3421 cggtaagatc ttcaaatata tcttttatgg cctttgcctg cccgtccgct cttacatgca
     3481 tgaccgcgtt cttatgcatc ctgcttgttt tctgaggacc tttttcaaaa agcatctttc
     3541 ttatataaag ttcctgaacg gcatccccca caaaagccaa tgccgttgta tcccttttta
     3601 ataggtcttc cgtcattgct gccttactcc tttattatct gtactccctg gggagtgtct
     3661 tttaaagtga tgccctttgc tttgagcata tcccttattt catctgccct ggcaaaatct
     3721 tttttctgcc tggccagccg gcgctcctca atgagcttgt ctatctcggg atcaactgca
     3781 ccatcttctt cctgcccgtc ctgcaaaaag cccaaaacat tgcagaattc cataagtgtt
     3841 tcaaggcact tctctgcaaa ttctctgctg acattctgct ttacagccgt attgatttcg
     3901 gtgatgagtt caaagaccgc tgttatggca tccgcagtgt taaggtcgtc atccatggcg
     3961 gatatgaaca tctgcctgta tttatccatg tcttctattt tctttttttc ttcttcagtc
     4021 atggtgccgc tgccgttttt gatcaggtgc ttaagattaa gacttgcatt tcttattctt
     4081 tccagactgt tttttgcctg ttccataagt tcgtcgctga aatttatggg gttcctgtaa
     4141 tgtccggaaa gcagaaagta ccttattacc tctccgctgt aagatcttag aatgtctctt
     4201 acagtaaaaa agttgcctct ggattttgac attttctcat tgtctatggt tatgtagccg
     4261 ttgtgcatcc agtattttgc aaaaggtttg ccgttatggg cttctgactg ggccacttcg
     4321 ttctcgtgat gaggaaacgt aaggtcctgt ccacccgcat gcagatctat tgtgtctcca
     4381 agatactttt ttgacatggt ggaacattct atgtgccatc cgggcctccc catcccccag
     4441 ggtgactccc atgctatttc gtcgtccttt tttcttgctt tccagagagc aaaatccagc
     4501 gggtcttttt tcttttcttc aaccattatc ctggctcccg attccagatc ctctatgttc
     4561 tgttttgaaa gcttaccgta atctttgaat tttctggcac tgaaataaac gtctccgtcc
     4621 acctcatagg catatccctt atctattaga tcttttacga attttactat ctcatcaata
     4681 ttttctgtta ctttcggatg aaaatccgcc ttggccacat tcagagcttc ggcatctttg
     4741 taatactctt cgatgtactt ttcacttatt tcagaggcgc tcactccttc ttcttttgcc
     4801 ttgtttatta tcttatcgtc cacatcggtg aagttctgaa caaatcttac tttttccccc
     4861 ctgtattgaa gatattttct aagggtatca aaaacaacaa aaggccttgc attgccgata
     4921 tgaaaataat tgtataccgt tggtccgcag acatatattt tgatctcact gctgtcggcg
     4981 ggaataaatt cttctttact tctcgtcaga gtgttgtata ttttcatttt ccagctcctt
     5041 aactttttgt tcaagcatta caatacgtct tctcagacat tccatttcaa ctgtggttgg
     5101 atccggaaga tccacctgat tcatttcttc tgtatgctgt cccggtctct ttattattat
     5161 tccggggact cccaccactg tgcagccgtc aggcacttct ttcagtacta ccgatccggc
     5221 tcctatcttt acatcgtttc ccactttgaa gggaccaagt acctttgctc cggaactcac
     5281 tatcactctg tcaccgatgg tggggtgcct ttttcccgta tccttgccgg tccctccaag
     5341 ggtcacaccc tgatagatgg aaacgtcatc tcctatttca gttgtttcgc cgatgaccac
     5401 agacatgccg tgatcaataa agcatctccg gcctattatg gctccgggat gtatttctat
     5461 cccggtaaaa aatcttgtca gctgagaaat tatcctggcc agaagcttga tattgtgctt
     5521 ataaagaaaa tgagcgggta tatgccacat ccttgcccat attcccggac agcacagaaa
     5581 cacctcaaat ccgtttcttg cggccgggtc tttttcaagc acagttttta tatcttctct
     5641 tgtggtttta aaaaaagcca ttttttctcc ccgttgatac acatatcccc cgtatatcaa
     5701 acttttgatc tgtaaatttt cttaatgccc gattctatta taagtatttc attgggtcta
     5761 ccgctgtgga aaggctggat cccctccata tctcaaaatg gagatgagga gatgaagcat
     5821 tgccgctgga tccgacttca gctattttct gtcctctttt tacggattgt cctgctcgga
     5881 cattcactga ctggcagtga gcatatatgg tagtcatacc gtttttgtgc ctcaccttta
     5941 ctattattcc ccatccgtat cctgattctc ccgcctgaag cacataaccg ctttgtgacg
     6001 catagaccgc agtcccgtat ccggctccta tatctatgcc gtcatgaaaa gccccgcctc
     6061 ttggtccgaa tcccgatata acattcattg aggaaagggg tctgccgaac tcgcctgatc
     6121 ctgtgttctt gagatcggcc cccgcagtac ccacaagtat cactctgctg ctggccttgg
     6181 acaaaacatt cttctcgatt atctctttgc ttacgacttt accattttct tttattattt
     6241 ttgtctttat ttctgttttg ccgctggatc cgtttttctt tatcttctct gttcccgctt
     6301 caagggaagt cacacttttc ctgatggtct tgcatttggt gatcttgatg tcttcacaca
     6361 attctcccga gatcactgta agagaatttc taccctcagt aagttctttt atcgcttcat
     6421 caatggtaaa aagttcaggt gcattacacc cgttatctgc atcaagcttc tcgattttta
     6481 cttcttcttt gaacttcaga aaaagtattt tgttttcttc attaatatac tgtttcttta
     6541 tgccttctat tgcaagcatg gcagcttctt cgctactaag gatcgctgct tttttgtctt
     6601 ctacttttat tacccatgga tgctgcatta cctgtcccgt tgccgttatc agaggtttat
     6661 cgattggctc ccctgtcatc agcaatccca gtgcaataga aaaaacaaaa acaataagcg
     6721 ccgctgctga tatctttttt ttgtgatgct tttttattaa gtccatcaac ttctccatca
     6781 acttctccct atatttgata tttttcttaa atacaagtcc agacaggttt tgacctgtct
     6841 ggaatattat acattatcga attggcataa tcaataaatc acagataatt gtaaggattt
     6901 ctggctacgc cgttaactct tacttcgtaa tgaagatgtg ttcctctggc gtttcccgtg
     6961 gctcccatgg aagcgatctt ctgtcccctg gaaacccttt gtccgacttt tacatgaacc
     7021 ctgctgcaat gggcatatct tgtctccagt ccgcctccgt gacttatttt tacgataagt
     7081 ccatatgttc cagagtaaca ggcctgtgtg acttttccgc tctcggacgc ataaatggga
     7141 gttcccatgc ttcctccaag gtcaatgccg taatgcatcc ttccccatct cataccgaat
     7201 gaagaaaggt gtctcatgct cgaaagaggt ctgcccagtg taccgctgtg attgtctata
     7261 gaacctcctg agcttgagta tgaggagccg taagaagatg acgatgatga acttgcggcg
     7321 gtgcccacaa gcacaacttt agcttttgct ttttttgttg tctgctcatc cagcacttct
     7381 ttttcgatca ccttgccgtt ttcttttata agttcggtga ctacctcttt ttcaccttta
     7441 gctccctcgg tctttacttt ttttgttccg gccggaagag aatcggtttt tttgtatatg
     7501 gttttatatt tggtttcttt ttttgcagta catatttcct ttgccgcaac tgttactgca
     7561 ttctcttcag tgatcttaga tattccttca tctatatcac atatgtccgg ttcctgttgg
     7621 ccgtttttta tatccatctc acagatcttt atatcttcct ttattttgac atctatgatc
     7681 tgattttttt cacatacata attctgcttt actccgtcca tgataagttg ggctgcatcc
     7741 ctgctccgtg ccactatctt atcttcccct tcaacggata tgacccaggg agtttccatt
     7801 acttctcctt ctgcagatac aaaaggtctg tcgattgcct gagaagttga agcccaccct
     7861 acggtcaggg agaaaacaaa aatgaccgct gcgaaaactc cgagtttttt cttttgcggt
     7921 aaagtaaggt gaaatatatc tgtttcattc aaaaacgaac gcagtccgtt ttttatccca
     7981 actgctgtgc cttttatacc ggcccttctt atgtcaagga ccctgttttt gagattcttt
     8041 attatttctg tcggtgccaa taaagttttg actcttccca agtcttttat tcttatccta
     8101 tcccctgccc ccgttaaaag tcttttgacc gtaatgacca gagatgatcc ggcctttatc
     8161 gccttttctt ttaacattgg tactcctctt tctttgttat caataattca catttcacat
     8221 tcaaggcctt tttatgacct ttttatgttt tactataaca aaaggttaca acaatgtcac
     8281 ttttctgtca agtgttgttt ccatgaaagt ctgaaccttt tgtgggtacc agaccaaatt
     8341 catctgccat ttgaagaaaa atccggtttt ctttttgtga atgctcagtg tatagacatt
     8401 caagcccgta aagacccata tccttcattt tgcggagaag tttttccaga ttttcttcga
     8461 attcagagct ttctctttcg cctatatgtc ttatctttgc cggatgagcc agcaccgcct
     8521 ttcctcccgc accattgata agacttatgg ccaaggccgc tgttattttt tcttttttta
     8581 tgcttcttat atctttttga tcaaatattc cccccggctc aaaagcatct ttttcgcatt
     8641 taatgtatcc tttgttcacc atcgcccttg ctatctgcgg cttgcccaaa taatcctgtc
     8701 cttttctgaa aaccaaatct ttttcttcca ggtcaaaccc tttttttctt aaaagatcca
     8761 ggagtttttt gtttctttct tttctccatc tccttgccct gtggcaggct ctgttaagct
     8821 ctttgttttc ataatcaata aaataaccga ggatatgtac tcctttgcca tcagccgcag
     8881 ccgacagttc tatccccggt attacttcga tgttgtattt tctgcctgct tccagagcct
     8941 cctgaatgcc ggaaacacca tcgtggtctg tgacggatat tttattcagt cctttgttct
     9001 ttgcatatct aacaacctct tccggagaca ttttgccatc ggaataccat gtgtgaaggt
     9061 gcatgtcggt tttttctcct gtaagcattt tacctcctga acatatgatg tcttcctgca
     9121 acagctaaag ttatataaag tccgataccc atcattatgg ctgcaacaag gatggaaaac
     9181 agcgaatagc gccacataaa agaagtatcc aggatcttga caaggagatc gtctttggga
     9241 aagttgaaag agctcaggtc tccgtgtatg gcctggcgca gcggttttac ggccactgtc
     9301 aaagtgtcga aaatagccag cacagcaaac ccgacaatag aataaaaata atttctcaga
     9361 agcagatctt tttctttttt tctgtaaaga tatatgtata tccctatcac aagcaacagt
     9421 atgactccgg cggcatatgc ctccttatca aggttatatc ttatattctt tacagtcagg
     9481 gcatctattt cgttgaaaag ttcttcaggc atatattcca ggttctcttt catccggaat
     9541 tcatcggtct tgcccatcat atagtcaccg aaaagtccga ccacatcttc attttcggca
     9601 taaaccatct cttcactaag gatctctgtg gaattcagat gaaaactata aagggctgtt
     9661 gtacggagca aaatattgtc cccggcatac atgacagcaa cagggatcat aacaataagc
     9721 agcggtgcca tgatttttgc ccacggcacc gattttctct ttttattctg cttccaggga
     9781 ttttttcttg agttccttct aaaactgcgt ttgagccctt caatacggct ggccaaagtt
     9841 tttttctttg gaactatcct gggatccctt ttggtcctct taaacatatt tgccattttc
     9901 aatctccgta ttgatcgatc tatgaaaatg tcgatcagcg ttcttcacgg aaatacggat
     9961 tgctcagatc tcccggagga agatcctcct tgcggttttt gtgtgtgctt ataattacta
    10021 ttattatagt tgcaagataa ggtatcatgt ccaccagata ctgagaaaca cttattccca
    10081 tggcctgcaa tctgaatcct attatgctca gtgctccgaa aagaagagcc cccagaaaac
    10141 cctttatgga tttccatgat gcaaagataa caagtgcaac tgctatccag cctctgcctg
    10201 ctacaacatt ttcctgccaa acgggtatag tcccaaggga catatatgca ccgccaagtc
    10261 cgcacagggc tccacctatt attatattag catatttgta aagagaaata tttattcccg
    10321 atgcatcggc cgccgcagca ttttcgccta ctgcccgggt attcagccct attcttgtct
    10381 tattgtaata gaatgccgtg actgccacaa gtataaaacc catataaaca agaatatcct
    10441 gatcaaaaag tgcccggcct ataataggta tctgagaaag cccgggaatg gcaatactgt
    10501 caaaggcatc tcttacaaca ggcgtaaggg gcactcccac ataacttctt cccaccaggt
    10561 ttgcaaaccc ggttccgaaa atcgtaagcg taagtcctgt taccacctga ttcgttctta
    10621 atgaaaccgt aagcaccgca aatatggccg caccggaagc acccgccacg catgccgcca
    10681 aaacggcaat ttggggactt cctgtggtat gacccgctat gaatccaatc acagctccca
    10741 taagcatcat gccttcaaca cccagattaa ggttaccgac tttttcagtc attatttcgc
    10801 cgatcgtagc atatagcaaa ggcatcgccg caactatggc tgccgccaga aaactttcca
    10861 tactatgcca cctccttttt tgatccgggt ctctttttga atgagacttt atactgcatg
    10921 aagaattcgc tgccaagaac gaagaaaagt atcacgccct gtatcatatc cgccaccgat
    10981 gacgggattg acatggcaac ctgtatatat gcaccgccct gcagaagcat ggcaaataat
    11041 atgcacacga tcatcactat tggcggagac aatctgccaa gccaggccgt tattattgcg
    11101 gtaaaaccgt accctgcgga aatgctgtat gtaagagtcc tttccacagc agagacctgt
    11161 atcatcccgg caagtccgca aaggcctccg gaaaccagca tggcacttat tattatgcct
    11221 ttaatattca tgcctgcata tcttgccgtt tccaggcttt cgcccacaac agatatctcg
    11281 taaccttttt ttgtatagtt tatataaaca tacaaaaatg ccaccgaaag gatcgccact
    11341 acccatccgc tgtgaatccc cagaaagtcc ggaagctgag cattctggct gaaggtctcg
    11401 atctttggga atcctcccga attcggatct ttccacggtc cgaactggag atatgtaacg
    11461 aacttgatgg ctatatagtt cagcatcaat gtaaatatag tttcgttggt accgaaacgc
    11521 gccttgaaaa aagcaggtat aaaggcccat atcgctccgc acagtataga tgctgccatc
    11581 atgcaaagaa tcataacggg tgcgggaaga gtgtccggga aattaagtgc caccagagtc
    11641 gctccaaagg ctcccatcat tatctgtcct tcaccgccta tgttccagaa cttcattttg
    11701 aaagcaacga ctatgcccat ggaaacaatg atcagaggta tggactttgt tatggtctgc
    11761 tctatcctca tgaccgagcc tatcgatcct tccactatcc tgtagtatgc ttccagggga
    11821 tcatatccca tggcaagtat cactatccct gcacaagcca gagaaagaag aatggcgatc
    11881 acctggatca ctgcttcctg tttcttcgga agagctgctt ttttagttat ttttatcatg
    11941 ctttgcctcc tcttccaaag atccgcctgt cataagcagt cctatgtcct ccttggtgac
    12001 ctcatccgga tcgactattc ctgttatttc tccctggcaa aggaccatta tcctgtcaca
    12061 aaggctcatg agcacatcca gatcttcgcc gataaacagg actccaacac ctttttgttt
    12121 ctgttcattc aaaagatcat atattttgta tgatgcacca atatcaagtc ctcttacggg
    12181 atatgccgtt ataagcactt ttgggtctga atctatctca cgccccaata gtactttctg
    12241 tatgttcccg ccggaaagtt ttttgaccgg agtatatata tcaggcgtca ttatatccag
    12301 agctttcact attttttccg cttttctggc gcagggacct ctttttagga aaatccccgg
    12361 ctggccctga tagtctttca gcactatgtt atgcactatg tccatgctgc ccacaagacc
    12421 catgcccagt ctgtcttcag gtatgaatcc cattcggata cccagcttta ttatatctgc
    12481 gggagttttg cccacaatat tttctccctg gaaaaatatt cgccctttgt tggtccttat
    12541 caggccggcg atacactcgc agagctcttt ctggccactt tttgccacac ctgcaacacc
    12601 caggatctct ccgccctcaa gttcgaagct tatgttttcc agagcatttt tgccgtcctt
    12661 gtcctgcacc tcaactccgt ccatggtcag cagtacccct gcctcctgtc ctttttcttt
    12721 ttttatggcc agatctactt ttttgcccac catcatctct atcagggtcc ccacatcaac
    12781 atcagctgtc tcaacagtgg ccacagatct gcctttacga agtaccgtca cccggtcgct
    12841 tatttccatt acctcgttca gcttgtgagt tattataaca acagcgcatc cctgatccct
    12901 catatttctt attatatgaa agagtttttc gatttcctga ggagtaagga ccgctgtagg
    12961 ttcatccagt atcagtatat gtgctccccg atagagcacc tttatta
//
LOCUS       DAONHK010000019        12808 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_3842_length_12808_cov_5.595703, whole genome shotgun sequence.
ACCESSION   DAONHK010000019 DAONHK010000000
VERSION     DAONHK010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 12808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12808
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3842_length_12808_cov_5.595703"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="PLM92_01420"
     CDS             <1..531
                     /locus_tag="PLM92_01420"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015555837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HPR23832.1"
                     /translation="PTHYVEQISETDTLVRAVNILPLEVIIRNVAAGSFSKRYGVEEG
                     TPLAKTVLEFSYKSDELGDPLINDDHILALKLATFEDLDTVRGYAFMVNDALKEFFLE
                     KGLKLIDFKIEFGKCIAGDEDDEPGDIILADEISPDTCRLWDAVTNEKMDKDRFRRDL
                     GNLEQTYKEVMERIKK"
     gene            544..1593
                     /gene="purM"
                     /locus_tag="PLM92_01425"
     CDS             544..1593
                     /gene="purM"
                     /locus_tag="PLM92_01425"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008537821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HPR23833.1"
                     /translation="MKSSEKLTYRDAGVDTLEGQRAVSLMKEHVRKTFDENVLADLGS
                     FGGLYELKGADIKEGSEPVLVSGTDGVGTKLKLAFMTGNHSTVGQDCVAMCVNDVLCQ
                     GARPLFFLDYIATGKVEAEKIAEIVGGIADGCREAGCALIGGETAEMPGFYADDEYDM
                     AGFCVGIVDKKKIITGEKISEGDRIIGLFSSGIHSNGYSLVRKVFFDENGFKLQDQIP
                     ELGETLSEALLRPTRIYSKACRAVCDFDIKGIVHITGGGFFENVPRVLPKDLSAEFDT
                     TAWEPAAIFRLIKKLGHMEEREMFSTFNMGIGMMFFAGSEDAGKILTALKEKGEDAAI
                     IGKVVKKQTDEVTLK"
     gene            1660..2247
                     /gene="purN"
                     /locus_tag="PLM92_01430"
     CDS             1660..2247
                     /gene="purN"
                     /locus_tag="PLM92_01430"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010240310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HPR23834.1"
                     /translation="MKKTKISVIVSGGGTNFQALAEAVKNGSIENAEIVRLISGSEKA
                     FALQRAKTLGIPSKVIKDTKDPAEILKLLEEAETDLVVLAGYLKILDPEIISAYKRRI
                     INIHPALLPKFGGHDMYGLNVHRAVIKAGEKESGATVHYVDEGVDTGEIILQRKVPVM
                     PCDTPEELAARVLETEHEILPLAVKEVIAEWKEKR"
     gene            2248..3726
                     /locus_tag="PLM92_01435"
     CDS             2248..3726
                     /locus_tag="PLM92_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008725232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HPR23835.1"
                     /translation="MGGIFGIVSNEDCVMDIFFGTDYHSHLGTKKGGMCILGDSGFNR
                     TIHNIENTPFRSKFEDDITEMRGKAGIGAISDGDPQPLTVLSRQGDYAITTVGRINNK
                     ADLVKELISDGHGQFMSMSGGGINNTELVAALVSQGKNIEEGIKNAQKRIEGSVTMMI
                     LTKEGLYAARDKLGRTPLIIGKKCGDSYCAASESFAFINIGYKYKRELGPGEIAFLTS
                     TEERTISPPGDKMRICTFLWTYFGYTTSIYEGKNVEKVRNRNGGILARGDRDLEVDYV
                     AGVPDSGIAHALGYSNASRIPYSRPLIKYTPTWPRSFMPQNQKARNLIAKMKLVPVFQ
                     IIKDKRFVLIDDSIVRGTQLSETVAYLIENGAREIHVRSACPPIMYGCKYLNFSRSVS
                     DMELITRRCIRELEGMDEAAGVNEASGNPAGSDSEKQSDIPPEVLLEYADASSERHRK
                     MVSLIEKKLNFDSLRFQTLEDVIESVQVDRCKLCTYCWNGKE"
     gene            3932..5674
                     /gene="purH"
                     /locus_tag="PLM92_01440"
     CDS             3932..5674
                     /gene="purH"
                     /locus_tag="PLM92_01440"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HPR23836.1"
                     /translation="MRAIISVSDKTGIVDFARELAKMGTEIISTGGTKRKLEEAGLAV
                     KGVEEITGFPECLDGRVKTLHPAVHGGILARRDIPSHMQKLKELHISPIDIVAINLYP
                     FKKTIMKPDVTLEDAIENIDIGGPAMLRASAKNHKDVVVICDPADYKKVTEELAKSGE
                     VSLETKYRLALKVFRHTAAYDAMIADHLRKQLPNEKKLSSDKTGAELPDDLTLTFEKI
                     QTLRYGENPHQEAFYYREIKPSEGSLVKAQKLHGKELSFNNINDTNGALECLKEFPEE
                     VPAAVAVKHANPCGVATGKTIFEAYRKAHDCDPVSIFGGIVALNRPVDKETALEMNKI
                     FLEIIIAPDFDEDALEILKQKSNVRLLKLPGICEATPAWEIDIKKVSGGILVQSKDRG
                     LFDVKNAPRPDQPASGGKPESQIKDQAADKAGDQTGDKPQGQVKAEGNNRASSAGNLV
                     YDDQLMVATKKSPTEAELRDMEIAMKVVKHTKSNGIVFVKDGKTIAIGPGQTNRIWAV
                     ENCIKRCLSDMKGSVMASDAFFPFSDCVEAAAEAGATAIIQPGGSIRDKESIEKADEL
                     GISMVFSGMRHFKH"
     gene            5758..7080
                     /gene="purD"
                     /locus_tag="PLM92_01445"
     CDS             5758..7080
                     /gene="purD"
                     /locus_tag="PLM92_01445"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002842528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HPR23837.1"
                     /translation="MRILIIGGGGREHAIAWKLAESEKVTKLYCAPGNAGIANNVECV
                     PVKDDDIEGMCDFAVSAEIDMAVIGPEVPLAMGMVDALEDAGKKAGKEIMGFGPNKKC
                     SQLEASKSFTKDFLTKHKIPTAGYREFTDKQELLENIGIFGFPMVLKADGLAAGKGVI
                     IAANEEEARKGIEALMGSRDFGTAGDKVVVEEFLRGVEASVLCFVDEKTIVPMESAQD
                     YKRIFSGDEGPNTGGMGTYSPSLVFDEKLEKRIRREILDPTLEGFRADGLDFKGVLFI
                     GLMIEEDGSPKVIEFNNRFGDPEAQSILPRMETDLTDVLLAVCKNRLSDIKIRWSEKK
                     AVCVVAASGGYPGEYLKGIEIKGLAKNGQIYGSDAVVFHAGTAMDHICHGSYCGQAFV
                     TAGGRVLGVTALGETHEEARKKAYENIEKISFEGMQYRNDIGLITPPK"
     gene            7729..8130
                     /locus_tag="PLM92_01450"
     CDS             7729..8130
                     /locus_tag="PLM92_01450"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HPR23838.1"
                     /translation="MIIRKRKTKIYIFIASMILIIVFFLIVLGLRAYCVAGKSMEPAV
                     PFGSIVFTIITEEPKQKLKKDDVIIFSSSKTTMPIMHRICSIEEDGVVTKGDNSNAVD
                     GRIPWIRIKSKVVFHIPAAGYPILFIQNMFK"
     gene            8164..8937
                     /locus_tag="PLM92_01455"
     CDS             8164..8937
                     /locus_tag="PLM92_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPR23839.1"
                     /translation="MGHNKSINIIFISLVITAVITILISVGAWLIDAEGFTNSFRVDA
                     DADTRYTLATSENNNEGEIILNGTDQSINRITLSGTGTTSVTSDGEGGVNIHTEPCLN
                     NIFPVGSIYMSANNQNPEDTLGGEWVSWGSGKVPVGVDALDTDFDTAEKTGGEKSHKL
                     TAAENGPHYHTGPLHTHGPGSGARFLNYTGTLSAETVGAIEGSGQKLAQILSGGSDVW
                     YGGTATAAAGNGNTGPSGLGDAHNNLQPYITCYMWKRIN"
     gene            9028..9693
                     /locus_tag="PLM92_01460"
     CDS             9028..9693
                     /locus_tag="PLM92_01460"
                     /inference="COORDINATES: protein motif:HMM:TIGR04088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SipW-dependent-type signal peptide-containing
                     protein"
                     /protein_id="HPR23840.1"
                     /translation="MKRKEDEKEIKTLERSRTKKKKSRFIPAFALMLMLIAGGVIALL
                     SDSETFQNVFGMSNLTVDISAEEMMASSEIGNEGSLIQYEDPAGVLPGDIVSKIPQVG
                     KVSENSLDCWVRAKVEFTQPENLSAEKTSLDQSALLGIPDSTWTVKNTADPGTIYYYL
                     NNGLTGQDISKTVLFTGVQIPDDWNETEIGKTPKVNVTFEAVQMENFQQDLTSDEPWG
                     NYN"
     gene            complement(9777..10397)
                     /locus_tag="PLM92_01465"
     CDS             complement(9777..10397)
                     /locus_tag="PLM92_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010680788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HPR23841.1"
                     /translation="MQYFLEGLLMGLAYVAPIGLQNLFVINTAISQKRSRVYATALIV
                     IFFDVTLALACFFGIGAIMEQSHLLEMIILLVGSIIVVKIGVGLLLPDKKTEESGDVD
                     IPILKVILTACVVTWFNPQALIDGSMMLGAFNATLPPEESSKFIIGVACASCLWFLSI
                     STILTFIGAKITDKILRIINVVCGVIIIFYGIKLFINFIQLIFTLI"
     gene            complement(10424..11035)
                     /locus_tag="PLM92_01470"
     CDS             complement(10424..11035)
                     /locus_tag="PLM92_01470"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018385112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HPR23842.1"
                     /translation="MTKYSGIFESPFGPIGIICSNDFVIEISWLNQRDVSISENRHSL
                     VRSAATQLDEYFSGKRSSFDLPLFIDDSLFVKDPVFIDDPLFANDPRYKSFGGTPFQR
                     RVWKALCDIPYGKTETYGQLANRIGCPGGARAVGSANAKNPVSIVVPCHRVIASGGRP
                     GEISRKNLGGYSAFGNGPKSQKGLALKQNLLEHEMKNKQNNMR"
     gene            11315..11926
                     /locus_tag="PLM92_01475"
     CDS             11315..11926
                     /locus_tag="PLM92_01475"
                     /inference="COORDINATES: protein motif:HMM:NF024587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HPR23843.1"
                     /translation="MKKQIITISREFGSGGHLVGKVLAEKMDVPYYDKSILDRIAEEK
                     GYSVEMMEEDEKRAKNSLLYSLTTAFGSAGTGPDYISINEKFFLAQFDYIRRIAKEGS
                     CVIVGRCADYVLREMPFVTNVFIYADKQQKFKRIVNEYGLSREDAEKGLRDVDKARAN
                     YYKYHTGQEWGRPENYHLCINSSDISLGGCTELILKYQEIKTK"
     gene            complement(12022..12537)
                     /locus_tag="PLM92_01480"
     CDS             complement(12022..12537)
                     /locus_tag="PLM92_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HPR23844.1"
                     /translation="MMDKKVIDLMNDQINKEFYSAYLYLDFANYYTDEGLDGFANWYK
                     VQAQEERDHAMLFAEFLQNNGIKVSLKAIGEPDVPMKKHADALKASFKHEQYVTALIH
                     KIYDVAFSKKDYRSMQFLDWFVKEQGEEEKNAEEMIRKYELYGSDAKGLYALNEELKS
                     RVYTPPSLVLD"
BASE COUNT         4228 a         2483 c         3121 g         2976 t
ORIGIN      
        1 ccgacacatt atgttgaaca gatatcagaa acggacactc ttgtccgtgc agtcaacata
       61 cttcccttgg aggtaataat aagaaatgta gcggcgggtt ctttttccaa aagatacgga
      121 gtggaagaag gtacgcctct ggcaaagacc gtactggaat tttcctataa aagtgacgag
      181 ctgggagacc ccctaataaa tgacgatcac atactggcac tgaagctggc cacattcgaa
      241 gatcttgata ccgtaagagg atacgcattt atggtcaacg atgccctaaa agaatttttc
      301 ctggaaaagg gacttaagct tatcgacttc aagatagaat tcggaaagtg tatcgccgga
      361 gatgaagatg atgagccggg agatataatc ctggcagatg aaatatcacc tgacacatgc
      421 aggctttggg atgctgtaac aaacgaaaaa atggacaagg accgcttccg gcgggatctg
      481 ggaaatttgg agcagacgta caaagaagtc atggagagga taaagaagta gaaaggatct
      541 tttatgaaat catctgaaaa gctgacgtac agggatgcgg gagtcgatac cctggagggg
      601 cagagagccg taagtctgat gaaggagcat gtcagaaaaa cctttgatga gaacgtgctg
      661 gcagacctgg gaagtttcgg cggattatat gaacttaaag gtgcagacat caaagagggt
      721 tccgagcccg tgctggtcag cggtactgac ggagtcggta caaaactgaa actggctttc
      781 atgacgggaa atcacagcac ggtgggacag gactgtgtag ccatgtgcgt caatgatgtg
      841 ctttgtcagg gagccagacc tttgtttttt cttgactata tagccacggg gaaagtggaa
      901 gcggagaaaa tcgcagaaat cgtcgggggg atagcagacg gatgccgtga agcggggtgc
      961 gccctcatcg gtggagagac tgcggaaatg cccgggtttt atgctgacga cgaatacgac
     1021 atggcgggtt tttgtgttgg aatagttgac aagaaaaaaa taataacggg ggaaaaaata
     1081 tccgagggcg acaggataat aggtcttttt tccagcggca tacacagcaa cggatactcc
     1141 cttgtacgaa aagtattttt tgatgaaaat ggattcaaac ttcaggatca gatcccggaa
     1201 ctgggagaga ccctttccga agccctgctc agaccaacca ggatatactc aaaagcctgc
     1261 agggcggttt gtgattttga tataaaagga atagtacata taacaggcgg cggttttttt
     1321 gaaaatgttc ccagagtact gccaaaggat ctttctgcgg aatttgatac gacggcatgg
     1381 gagccagccg ccatatttcg gttgataaaa aaactcggac atatggaaga aagagaaatg
     1441 ttttcaacat tcaatatggg aatcggcatg atgttttttg cgggcagtga agatgccggt
     1501 aagatactaa cggctctcaa agagaaaggc gaagatgcgg ctatcatcgg caaagttgta
     1561 aaaaaacaga cagatgaggt gactctgaaa tgatagcagg acaggcaaaa gaggcgactc
     1621 tgaaatgaca gaaggacagg caggggagga gagcatcaaa tgaaaaaaac caagatctct
     1681 gtaattgtat ccggcggcgg cacaaatttt caggcactgg cagaagcagt caaaaacgga
     1741 tccatagaaa atgcagaaat agtcaggctc atatccggca gcgaaaaggc cttcgcactt
     1801 caaagagcaa agaccctggg gatcccgtca aaggtgatca aagacacaaa ggatccggca
     1861 gaaatattaa agcttcttga agaagcggaa acggatctgg tggtgctggc gggatacctg
     1921 aagatacttg atcccgaaat aataagtgct tacaaaagaa gaataataaa catacatccg
     1981 gcacttttgc ccaaattcgg agggcatgat atgtacggac tgaatgtgca cagggcagtc
     2041 atcaaagccg gagaaaaaga aagcggagcg acggtgcatt atgtggatga gggagttgac
     2101 acgggagaaa taatcctgca gagaaaagtt cctgtgatgc cctgtgatac accggaagaa
     2161 ctggcggcgc gagtccttga aactgaacat gagatattgc ctctggctgt gaaagaagtc
     2221 atagcagaat ggaaggagaa acgttaaatg ggtggaatat tcgggatagt atcaaacgaa
     2281 gattgtgtaa tggacatatt tttcggcacc gattatcact cacatctcgg aacaaaaaaa
     2341 ggtgggatgt gcatacttgg ggatagtggt ttcaacagaa ctatacacaa tatcgaaaac
     2401 actcctttca ggagcaaatt cgaagatgat ataacagaga tgagggggaa agccggcata
     2461 ggagccataa gtgacggaga cccccagcct cttactgtac tgagcagaca gggggactat
     2521 gccataacca cagtgggcag aataaacaac aaagcggatc ttgtaaaaga gctcatatcg
     2581 gatggacacg gacagtttat gtcaatgagt ggaggaggca taaataacac cgagctggtg
     2641 gcggcccttg tttcccaggg caagaatata gaagaaggaa taaaaaacgc tcaaaaaaga
     2701 atagaaggtt ccgtgaccat gatgatcctg accaaggaag gcctttatgc ggccagggac
     2761 aagctgggca ggacccccct tattatcggc aagaaatgcg gagacagtta ttgtgccgct
     2821 tcagaatcct tcgcctttat aaacatagga tacaaatata agcgtgaact gggacccggc
     2881 gaaatagctt ttcttacttc cacggaggaa aggaccatat ctcctcccgg ggataaaatg
     2941 cgaatatgta cttttctttg gacttatttc ggctacacca cttccatata tgaagggaaa
     3001 aatgtagaaa aagtaagaaa tagaaacggt gggatactgg ctcgcggaga cagagatctg
     3061 gaggtggatt atgttgcggg agttcccgac agcgggatag cccatgctct tggatattca
     3121 aacgcatccc ggatccctta ctcgagaccc cttataaaat acacacccac atggcccagg
     3181 agctttatgc cccagaacca gaaggcaagg aatcttatcg caaaaatgaa actggtaccg
     3241 gtttttcaga taataaagga caaaagattt gttcttatag atgattccat agtcagagga
     3301 actcagcttt ccgagacggt ggcatatctc atagaaaacg gtgccagaga gatacacgta
     3361 agatctgcct gtccacccat aatgtacggc tgcaagtacc tgaacttttc cagatccgtc
     3421 agcgatatgg agctcataac caggcgatgc ataagagaac tggagggcat ggatgaagcc
     3481 gccggtgtga atgaggcatc cggaaatccg gcggggtctg atagtgagaa gcagtcggac
     3541 ataccgcctg aggtattgtt ggaatatgcc gatgcatcca gtgaaaggca cagaaaaatg
     3601 gtgagcctga tcgaaaagaa actcaatttt gacagcctcc ggttccagac actggaagat
     3661 gtaatagagt ccgttcaggt ggacagatgc aagttgtgca cctactgctg gaacggaaaa
     3721 gagtgatggc atcaaacatg agaaaactgc atcattgaaa aatgtgtgaa tgagaaatgt
     3781 tttatgaaaa atgattataa atgatccgac aaaaagagag aaacaaaagc tataaacaaa
     3841 aatgacaaac aaaagcgaca aacaaaagca atcagaaaat gaaaatgaat caaaagcaaa
     3901 ataataattt ataaaaatga aaggagtagc aatgagagcc attataagcg tatccgataa
     3961 aaccggcatc gttgactttg cccgtgaact tgccaaaatg gggacggaaa tcatatccac
     4021 cggcggcaca aaacgaaagc ttgaagaggc cggacttgcc gtcaaaggtg tggaggaaat
     4081 aacagggttc ccggaatgtc tggacggaag agtgaagact cttcatcctg cagtgcacgg
     4141 aggtatactg gccaggaggg acattccttc ccatatgcaa aaattgaaag aactccatat
     4201 cagccctata gatatagtgg cgataaatct ctatcctttt aagaaaacta taatgaaacc
     4261 cgatgttact ttagaagatg ccatagagaa tatcgacata ggcggcccgg caatgttaag
     4321 ggcctcggcc aaaaatcata aagacgtggt ggttatatgc gaccctgcgg attacaaaaa
     4381 agtgacggaa gagctggcaa aaagcggaga agtttcactt gaaacaaaat acaggctggc
     4441 cctcaaagtt ttccggcata cagccgcata cgatgccatg atagcagatc acctgagaaa
     4501 gcagctgccc aacgaaaaga aactatccag tgacaaaacg ggagcggaac ttcccgatga
     4561 ccttactctg actttcgaaa agatacaaac tctgcgttac ggagaaaatc cccatcagga
     4621 agctttttat tacagagaaa taaagccgtc tgaaggatca ctggtaaaag cccaaaaact
     4681 tcacgggaag gaactttcct tcaataatat aaatgatacg aatggagcac tggaatgtct
     4741 caaggaattt ccggaggaag tgcctgcggc ggtagcagtc aagcatgcaa atccctgcgg
     4801 tgtcgcaacg ggaaaaacaa tttttgaggc atacaggaaa gctcacgact gtgatccggt
     4861 ttcgattttc gggggaatag tagcactcaa caggcctgtc gacaaggaaa cggcccttga
     4921 aatgaacaag atatttcttg agataataat cgcgccggac ttcgacgaag atgctcttga
     4981 gatcctgaag cagaagtcaa atgtaagact gctcaagcta cccggtatat gcgaagctac
     5041 gcccgcatgg gaaatcgaca tcaagaaagt ttcgggagga atactggtac agagcaaaga
     5101 ccgtggcctt tttgatgtca aaaatgcacc aagacctgat cagcctgcat ccggaggcaa
     5161 acccgaaagc caaataaaag accaggcagc agacaaagcc ggagaccaaa ccggagataa
     5221 accccaaggc caagttaagg cagaaggaaa taatcgggct tcttcagccg gcaatctggt
     5281 atatgatgat cagctgatgg tggcaacaaa gaaatctccc acggaagcgg agctgcggga
     5341 catggaaata gccatgaaag tggtcaagca tacaaaatcc aacggtatcg tttttgtaaa
     5401 ggatggcaaa accatagcca tcgggcccgg acagacaaac aggatatggg ctgtggaaaa
     5461 ctgtataaaa agatgccttt cagatatgaa aggctccgtt atggcttcag acgcattctt
     5521 tcctttcagc gactgtgtag aagctgccgc agaagccggt gcgacggcaa tcatacagcc
     5581 cggcggatcc ataagagata aggaatccat cgaaaaagcc gatgagctgg gcatatcaat
     5641 ggtattttcc ggaatgagac atttcaagca ctgaaacgtg gggaaatatc aaggctcgac
     5701 ccgggttcat atagcataaa cctggacaaa tacatattaa tagaacggag aaataatatg
     5761 aggatactga taataggcgg cggaggtcgt gaacatgcca tagcatggaa actggcagaa
     5821 agcgaaaaag tgacaaaact ttactgcgct cccggcaatg ccggtatagc aaataatgtt
     5881 gagtgtgtgc ctgtaaaaga cgatgacatt gaaggcatgt gcgactttgc cgtctcggca
     5941 gaaatagaca tggcggtcat aggtcccgag gtgcccctgg ccatgggaat ggtcgatgca
     6001 ctggaagatg ccggcaaaaa agcagggaaa gagataatgg gtttcggacc caacaaaaaa
     6061 tgctcacagc tggaggccag caaatctttc acaaaggatt ttcttacaaa gcataaaata
     6121 cccaccgcag gatacagaga gttcacagac aagcaggagc ttttagaaaa catagggata
     6181 ttcggattcc caatggtgct gaaggcagat gggctggctg caggaaaagg tgtcataata
     6241 gcagcaaatg aagaggaagc gagaaaaggt atagaagccc ttatgggaag tcgtgatttt
     6301 ggcactgcgg gagacaaggt ggtagttgag gaatttttaa gaggggttga ggcatccgtg
     6361 ctttgttttg tggatgagaa aaccattgtt ccaatggaat cggctcagga ttataagagg
     6421 atattttcgg gagatgaagg acccaacacc gggggcatgg gtacatattc cccttcactg
     6481 gtgtttgatg aaaaactgga aaaaaggata cgccgggaaa tacttgaccc cacattggaa
     6541 ggatttcgcg ccgacggtct tgatttcaaa ggtgtacttt ttatagggct tatgatagag
     6601 gaggacgggt ctcccaaggt cattgaattc aacaacagat ttggggatcc tgaagcccag
     6661 tcgatcctgc cccggatgga gaccgatctt acggatgttt tgcttgccgt atgcaagaac
     6721 cgtttgtctg atataaagat aaggtggagc gagaaaaagg ctgtttgcgt ggttgccgca
     6781 tccggcggat atcccggaga atacctgaag ggcatagaga taaagggttt ggcaaaaaat
     6841 ggccagatct atggctccga tgcagtagta ttccatgcgg ggactgccat ggatcacata
     6901 tgtcacggaa gttactgcgg gcaggctttt gttactgcag ggggaagagt cctgggggtt
     6961 acggctcttg gagagaccca tgaagaggca aggaaaaaag catatgaaaa catagagaag
     7021 ataagttttg agggaatgca gtacagaaac gacatcggcc ttattacacc acccaaataa
     7081 gctgtttcgg aacacttttc ccggaaaatg gagagtgcgg taaacgtggg agccggaaca
     7141 cttttcccgg caaacgccgc ccgtgggaat cgagcccctt ggaaaagccg ccccggcaac
     7201 agtgatcctc gggatccaaa agagaaacac ggcaaaataa tagcaaattc tattttttaa
     7261 ccctttgacc tcgtttttga gaggtcgagg gttaattttt tgtagaaaag cagggttttt
     7321 tggtcatttc agaataaaaa atcaatttgt atagctgaaa tccgtacata ttggcattaa
     7381 aacagcataa taaaaaaaat aaagggcgat tttggaaaga ctggaaacag aaaacagaaa
     7441 tttttaaccc tgaagcctta agaattgaga taaaagggtt aaaaatccta aacaagtctg
     7501 ttttttgctt tatttggaat caaccggaga ctgtatggcg gatatccaaa acataaaaga
     7561 aaaaaaataa cataatttac aaataatggt tgacaaatat ttccagtaat gctaccataa
     7621 ccataccaaa ggcaaaataa aagtacaaac agataccaac agaaacataa cgataccaaa
     7681 taatttattt caccatcaga caattatggc aagtcaaaag gggccgaaat gattatcaga
     7741 aaaaggaaaa cgaaaatcta catatttata gcatcaatga ttttgattat tgttttcttt
     7801 ttaatcgtcc ttggtcttcg tgcctactgt gttgcgggaa aaagcatgga acccgcagtt
     7861 ccctttgggt cgattgtctt tacgattatt acagaagaac ccaaacagaa attgaagaaa
     7921 gatgatgtga tcattttttc ttcttcaaaa actactatgc ccatcatgca cagaatatgc
     7981 agtatagaag aagacggagt ggtcaccaaa ggagataaca gtaatgcagt agatgggagg
     8041 ataccctgga tcaggataaa aagcaaagtg gtttttcata tacctgctgc agggtatccc
     8101 atcttattca tacagaatat gtttaaatga ttttctgata agccgataca gcaaggagaa
     8161 aaaatgggac ataataagag cataaatatt atttttatat ctctggtgat cactgcagtt
     8221 attacgattt tgatcagtgt tggagcatgg cttatagatg ccgagggctt cactaattct
     8281 ttccgggtcg atgcggatgc ggacacgaga tatacgctgg ctacttcgga gaataacaat
     8341 gaaggggaaa tcattcttaa cggaacggat caaagcataa atagaataac tttgtccgga
     8401 accgggacaa catctgttac ttctgatgga gagggaggag tcaatatcca tacagaacct
     8461 tgcctgaata atatattccc tgtgggcagt atatatatga gtgcaaacaa ccaaaatccg
     8521 gaagatacat tgggaggaga gtgggtatca tggggaagcg gaaaggttcc cgttggagtc
     8581 gacgcgctgg atacggactt tgatactgcc gaaaaaacag gcggggaaaa aagccacaaa
     8641 ttgacagcgg cagaaaacgg tccccattat cacacaggtc ctttgcacac tcatggaccc
     8701 ggctccggag caagattctt gaattatacc ggaacattat cagctgaaac cgttggtgct
     8761 attgaaggca gtggccagaa gctggcacag atattatcgg gcggcagtga cgtatggtac
     8821 ggagggactg caacggcagc tgcaggaaac ggaaacacag ggccatccgg cttaggtgat
     8881 gcgcacaaca atctgcaacc ctatatcacc tgttatatgt ggaaaagaat aaactgatcc
     8941 gtatttctgc cggcagactt tgaccaaaaa gcagtggctc agcacaaaag gatcaacaaa
     9001 taaaaatgat cacaaaggag gaaaagcatg aaaagaaaag aagatgaaaa agaaatcaaa
     9061 actttagaaa gaagcaggac taaaaagaaa aaatccagat tcataccggc atttgcgctg
     9121 atgctgatgt tgattgcagg cggcgtgatc gcactgcttt ccgacagtga aacatttcaa
     9181 aatgtctttg gaatgtcaaa tctaacggta gatatatctg ctgaagaaat gatggcaagc
     9241 agtgaaatcg gcaatgaggg ttcgctgata cagtatgagg atcctgcagg tgttttgccg
     9301 ggagacattg tttccaagat ccctcaagtg ggaaaagtat cggaaaactc attggattgc
     9361 tgggtcagag cgaaagttga gtttacacag ccggagaacc tgagcgcaga aaaaacatct
     9421 ttggatcaaa gtgctcttct ggggataccg gatagcactt ggacggtgaa gaatacagct
     9481 gatccgggaa cgatatacta ttacttgaac aatggactga caggacagga tatttcaaaa
     9541 acagttcttt tcacaggtgt tcaaatacct gatgactgga atgaaacaga aatagggaaa
     9601 accccaaaag ttaatgttac attcgaggca gtacagatgg agaattttca gcaggatctt
     9661 acaagtgatg agccttgggg aaactataac taaaaataaa agccggacaa ttgcagatca
     9721 gagaagaaaa accaaatacc aaatattgaa aactgacaag ggcaaaacag gaaaagctat
     9781 attagggtaa aaataagctg gatgaagttt ataaagagtt taataccgta aaaaataata
     9841 atgacaccgc acacgacatt tattatgcgg aggatcttgt cggtgatctt tgctcctatg
     9901 aaagttagaa ttgtagagat acttaggaac cagagacagc tggcacaggc cacaccaatg
     9961 atgaattttg aactttcttc aggaggaaga gttgcattga aagctccgag catcatggaa
    10021 ccgtcgataa gagcctgagg attgaaccac gtcactacac atgccgttaa aatgactttc
    10081 agaatgggga tgtcaacatc accgctttcc tccgtttttt tatcgggaag aagcagtccc
    10141 acccctattt ttacaactat aatgctgccc acaagaagaa tgatcatttc cagcaggtgg
    10201 gactgctcca ttatagcacc gatgccgaag aaacaggcaa gggccagcgt aacatcaaaa
    10261 aatataacta ttaacgcagt cgcataaacg cgactgcgtt tctggcttat ggccgtattt
    10321 attacaaaaa gattctgcag gccgatggga gccacatagg ccagacccat caaaagacct
    10381 tctaaaaagt attgcatttg tgttttcctg ttcttgccgg atattacctc atattatttt
    10441 gtttgttttt catttcatgt tcaagaagat tttgtttgag agcaagaccc ttttgacttt
    10501 ttggaccgtt cccaaaagca ctgtagccac caagattttt tcgggagatc tcaccgggcc
    10561 tgcctccgga ggctattacc ctgtggcacg gtacaactat agaaaccgga tttttggcat
    10621 tggcagaacc aacagctctt gctccgccgg ggcaccctat acggtttgcc agctggccgt
    10681 aagtttcagt tttgccatag gggatatcgc aaagagcttt ccagaccctt ctctgaaagg
    10741 gagttcctcc gaatgattta taccgggggt cattagcaaa taaaggatca tcaataaata
    10801 cagggtcttt aacaaataga gaatcatcaa taaaaagagg aaggtcaaaa gagcttcttt
    10861 ttcccgaaaa atattcatcc agttgagtag cggcactacg gacaagagaa tggcgatttt
    10921 ccgatattga aacatctctt tgattgagcc atgaaatttc aattacaaaa tcattggagc
    10981 aaataatacc tatcgggcca aatggactct cgaatattcc cgagtatttt gtcatggatg
    11041 tttcctttct tgatattgga gcggtcttaa cctggagcaa ctctatgtat gatcttgcgc
    11101 acaccctttt gtatcgtacc ggaaagattc ttcatatctt aatatacggg aattgacatg
    11161 ttttttgccg gacacttcag aagctatgcg tcggatgctg caaaatattt tcagtaataa
    11221 ttgtatcaca gaagataaaa atagtgtata atatatatac tcaaacttgt ttaagatata
    11281 gggatacaag ggaaaaatat tggaggacag ggttatgaaa aagcagataa taaccatcag
    11341 ccgtgagttc ggcagcggag gccatctggt ggggaaagtg ctcgccgaaa aaatggatgt
    11401 gccttattat gacaaaagta tactggaccg gatcgcagaa gaaaaaggct attcagttga
    11461 gatgatggaa gaggacgaga aacgcgcaaa aaacagtttg ctgtacagcc ttactactgc
    11521 tttcggcagt gcgggaacag gaccggatta catttccata aacgaaaaat tttttcttgc
    11581 tcaattcgat tatataagaa ggatcgcaaa agaaggttca tgtgtaatag taggaagatg
    11641 tgcggactat gtgcttaggg agatgccttt tgtcaccaat gtttttattt atgcggacaa
    11701 acagcagaag ttcaagcgga tagtaaacga gtacggactt tccagagaag atgctgaaaa
    11761 gggtcttaga gatgtggaca aggcaagggc gaactactat aagtatcata cgggacagga
    11821 atggggcaga cccgaaaact accatctttg cataaacagc agtgatatca gcctcggcgg
    11881 atgtacagag ctgatactga aatatcagga aataaaaacc aagtaggtca ttaaaaatac
    11941 tgatcagaca aatataaaag cagacaattt tttcgcttgt ctgctttttc atattatatg
    12001 cttgattcaa tgtcaaaagc tttagtcaag tacaagactt ggaggagtgt agaccctgct
    12061 cttaagctct tcattgagag catacagccc ctttgcatca gatccgtaaa gctcgtactt
    12121 cctgatcatt tcttctgcgt ttttttcttc ttctccctgc tcctttacga accagtcaag
    12181 aaactgcata ctgcgataat cttttttaga aaaagccaca tcatatattt tgtggataag
    12241 ggccgttaca tactgctcat gcttgaaaga tgccttaagg gcatcagcat gttttttcat
    12301 aggtacatca ggctccccaa tggctttcag tgaaaccttt atgccattgt tctgcagaaa
    12361 ctctgcaaaa agcatggcat gatctctttc ttcctgggcc tgtaccttgt accagttggc
    12421 aaatccgtca agcccttcat cagtatagta attcgcaaaa tccaggtaaa gatatgcaga
    12481 ataaaactct ttgtttattt gatcgttcat taaatctatt acttttttgt ccatcattat
    12541 taatacctcc gctttaaaaa ttttaatatg tttatcctat acccggacaa aaataaatca
    12601 aacaaaataa cagaggctat aaagacccgg caataagaaa actcaaagca aacagatcgg
    12661 atttacaaaa acctgatttt taggaaacaa aggaaatcag ccaagcccgg ggtttgtgaa
    12721 acaacggaaa tcggtcaacc ccggaattta taaaacaacg gaaagcagct aagcccggag
    12781 tttatataac cacagaaatc agttgata
//
LOCUS       DAONHK010000020        12677 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_3895_length_12677_cov_4.707416, whole genome shotgun sequence.
ACCESSION   DAONHK010000020 DAONHK010000000
VERSION     DAONHK010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 12677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12677
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3895_length_12677_cov_4.707416"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..619
                     /locus_tag="PLM92_01485"
     CDS             <1..619
                     /locus_tag="PLM92_01485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPR23845.1"
                     /translation="MKTLGAEMIMIDGAVDRKSIAAPDASDAVILATGAVLSRNMKKV
                     VQETAYVVSICSLPRLSDQKTRKLIEENQDKITLISGDRAKTLDLATGLNSGKFLQQE
                     MNDETEYIYMPGAFTKSVIADMGNAELKKTHFILKDPMKIFFDAIGWHQLMKKGTKVS
                     VLENIKVAAVTVNPVSPAGYSFGHEDLREAVSKALPEIPVIDVRL"
     gene            652..2298
                     /locus_tag="PLM92_01490"
     CDS             652..2298
                     /locus_tag="PLM92_01490"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPR23846.1"
                     /translation="MIKRLANVKTRTNTGFDHVMNGMELQTPFGTKRLKEQKPFFPGE
                     EASLRRELDRIGSLFDIAREDEKYISKISDIFMDMKDVEFTVERSKSSILTVVEIFEM
                     KCLLLKMEQLSSLFQKQSKKIPDEFILSDIRILLDELDPRGDRLNTFYIYEEFSKKLA
                     ELRNEKRMLETGVRKSQKERREKLHREKGIMLTPKFDCVVSKADEEAIRTFEAAPELE
                     RSCQDIISVTFQLVRTEKEIETIEKTEMLTAQIEEEEEKIREKISGRVWAFQKEIREN
                     CIRIGSFDLTLAKALYAIRHRCVKPEIIKEYAVSIKDGRQLQAEEILDSKGKEYMPVS
                     ISLDPGVTCITGANMGGKTVSLKMIGLTVLLAQYGYFVPCKSAKIGLSNFMQMLMGDS
                     QSLERGLSSFGSEMEELKEIFDHCTERSLIMVDEIASGTNPSEGLALTKSIIDFLKDE
                     LYISVITTHFDVGEGGGKVKNVQVTGLANADFTRLDRELRYANRKERISIIGKYMDYR
                     LSPAKEGKTIPKDAINIVKMLGIENETIENATKYLKGEKG"
     gene            2295..3851
                     /locus_tag="PLM92_01495"
     CDS             2295..3851
                     /locus_tag="PLM92_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006306965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 5,6-aminomutase subunit alpha"
                     /protein_id="HPR23847.1"
                     /translation="MKSKLNLDPKVIDSARSCASRIAHSMQEFIDVHTTVSTERTVVR
                     LLGVDGVDDVETPLPNIVIDQILEAGGLPRGAAYWMGNAMIQTGLDSQEIAEKISAGE
                     LNITELPTAGIEEAKEKIMPHVKDSLDKIKAQTAKREEYLATIGEGRTPYLYVIVATG
                     NIYEDVVQAKAAVRQGADIVAVIRTTAQSLLDYVPYGPTTEGFGGTYATQENFRIMRK
                     ALDEVGEEVGKYIRLCNYSSGMCMAEIAAMGALERLDVMLNDALYGILFRDINMQRTL
                     IDQYFSRVIIGYCGTMFNSGEDNYLTTDDAYEAAHTVLASQLINEQFAVLANIPEEQM
                     GLGHAFEMDPDIENGFLYELAQAVMAREIFPNAPLKYMPPTKYMTGNIFKGHIQDALF
                     NMIAVWSGQSLQLLGMPTEAIHTPHMHDRYLSIENASYIFNNARDIGDEVEFKENGIM
                     QQRAQKVLKEADELLHEIEKDGLFSTLEKGKFGGVKRPRDGGKGLSGVCLKDEKYFNP
                     FITIMLGGEK"
     gene            3851..4618
                     /locus_tag="PLM92_01500"
     CDS             3851..4618
                     /locus_tag="PLM92_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006306963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OAM dimerization domain-containing protein"
                     /protein_id="HPR23848.1"
                     /translation="MTDKTAVAQVDLKKVKPYGDTMNDGMTETAFTLPVPFGEEGNEA
                     AKILAKKMGLDEPNVVYSKDQGGGYTFFIVYGKCRHTVDYTEIKVTKVEVEIMDRIEC
                     AKFIEEKIKRDVVIVGASTGTDAHTVGIDAIMNMKGFHGHFGLERFDGIEAYNYGSQV
                     PNEVLIEKAKEVNADAIIISQTVTQKDIHIANLTNMVELLEAEGMRDKVILCCGGPRI
                     SHELAQELGYDAGFGAHTYAEHVASFILNEIVEKGRA"
     gene            4633..5313
                     /locus_tag="PLM92_01505"
     CDS             4633..5313
                     /locus_tag="PLM92_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016321248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HPR23849.1"
                     /translation="MKNKIMTAKEAIAGIKDGSSIMVGGFMCCGTPEILIDALVEKGT
                     KDLTIICNDAGVPGRGVGKLITNGQVKTLIASHVGLNPEVAQRMNTEDPDEKLECILV
                     PQGTLAERIRACGAGLGGFLTPTGVGTIVADGKKVINVGGKDYLLEEPLKADFAFLRG
                     SVTDKFGNTTYNATTRTFNPMMAAAADYVIVGACEIVEMGEIDPNNIVTSGIFVDAIV
                     GGEKQWQI"
     gene            5301..5954
                     /locus_tag="PLM92_01510"
     CDS             5301..5954
                     /locus_tag="PLM92_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016323720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HPR23850.1"
                     /translation="MADLKARIATRVARELKDGDVVNLGIGLPTKVANYLPGDVHIIL
                     QSENGIMGMGPAAEEGKEDVDIINAGAQYVTINTGAMYFDSATSFGIIRGGHVDATVL
                     GALQVDKHGNLANWIVPGKMVSGMGGAMDLVVGAKKVIISMLHTQKGAHKILDECTLP
                     YTAIKVVDMIVTEMGVMEVTDEGIVLKELHPDFTLEEIKEATGCRLIISPDLKPMED"
     gene            6153..6941
                     /locus_tag="PLM92_01515"
     CDS             6153..6941
                     /locus_tag="PLM92_01515"
                     /inference="COORDINATES: protein motif:HMM:NF019142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HPR23851.1"
                     /translation="MLGSLLKRSNLKYCARAFLLIISIAMIAGTTALAADEPAGKGHV
                     EVSIAVDDMEIHAKTAQMTVSEVIDSQDIPLGPDDMVTPAEDTVVEDGTKIVIKRILR
                     KEMTVTETVDYRTIKKQDSDLAYNEKKVVQEGSEGLDRLTYEVLYSRGKEVSKKEISR
                     TTIKKPRNRIIKTGYKDKFEVRAFAYTGGGTTATGTRARVGAIAVDPDVIPLGTKVYV
                     EGYGSAVAEDTGGAFIQGKSIDLYMNSTAACYSWGKRYVTIYIL"
     gene            complement(6994..8316)
                     /locus_tag="PLM92_01520"
     CDS             complement(6994..8316)
                     /locus_tag="PLM92_01520"
                     /inference="COORDINATES: protein motif:HMM:NF019585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HPR23852.1"
                     /translation="MEQKNGTKKIFLDDVYKKEHQSVITDVFSEGGYPVLVLGSTIFF
                     PGGGGQSCDLGETEGVPLRDVFEKDNEIFHVLEKSETLFSKGQSVLCRLDWARRFDNM
                     QRHCGEHILSGVFSREYGGVNRGFHMGSDYMTIDISLEKKDDEKENDNENNAQNSGMK
                     INEIDWEIAMRAQDLANEIIWQNKDISIHLFTDPEDANSLPLRKPADFDEDVSVVLIG
                     EKDNPSDCVACCGTHPHSSSEVGLVKIYKVEKYKGMFRIYFEAGARAMADYNKKHSIV
                     NDLCIKHSAGTEDLMGKIAAADTRVKVMRDELNTLRHSVITSRIEETAEILDKLTKGE
                     DPLCKGPFSVRGDILVCRWSDMKTDDLLNIGRPLSDRVKKLLVIVSQKDNTLLFFSDG
                     SVDCSKLVRENAGIYNGKGGGNATSSRAIFPKEEDMDLFIDLAEKHLR"
     gene            8671..9114
                     /locus_tag="PLM92_01525"
     CDS             8671..9114
                     /locus_tag="PLM92_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006599195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HPR23853.1"
                     /translation="MKYSLDVKTSSHTQMLDITKDIEKIVKESKIRNGTCTVFIPHTT
                     AAVTINENADPDVQKDFIKTINKIVPWEDDYLHMEGNSAAHLKASMIGFSEQIIIEEG
                     RLLLGTWQGIYFIEFDGPRNRKVWVGILADQKSANGRQETTDGSE"
     gene            9604..11148
                     /locus_tag="PLM92_01530"
     CDS             9604..11148
                     /locus_tag="PLM92_01530"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HPR23854.1"
                     /translation="MLKERITFKSELPVNVLVADIEEYPIHFHDELEMVFVLKGHVKL
                     RNGYYNYDLKQGDIFTLNDREIHSFSRIENQENMVMLLQMDLTYFSRYYNNLKNNFFV
                     TDMEDDDDESLDILRKFLAKIMMDILEKGFGYEQKVIESTHNLIASLQADFQYFVMED
                     GRFVNEVKKKGNKILAGRLNRITDYMYDNYYRKLTLKEIAEKEHLSIYYLSHVIKEAT
                     GLSFQDLLNFIRVEESEKLLLGTNKRIGAIAEETGFSAVRYYIKHFETWFHMHPAEYR
                     KKFTGKVSGRESGAKMRRAAPAEIEEALKQQVKGLYRDYFFRNAIKPIIVDLDIDKEV
                     SGTFGKSVMDELMQRDIMKQVSAPYIMMKNLGEEIIASEDNYLISADKDEAGKINALS
                     ILIYNFSEDLGRELGMVSSKEELINSLSVYEAEGEILVRCAGMTGDFRISRYRISRQN
                     CLMAIDSSGIHDKSHSAREILRGKLAAMPVVEFEEFHVADTISVRSTIKGFGAELILI
                     DKKTAK"
     gene            11258..12528
                     /locus_tag="PLM92_01535"
                     /pseudo
     CDS             11258..12528
                     /locus_tag="PLM92_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003889390.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
BASE COUNT         4288 a         2417 c         3034 g         2938 t
ORIGIN      
        1 aatgaaaacg ctgggtgcgg aaatgataat gatcgacgga gccgtagaca gaaaatccat
       61 cgcggctccc gatgcatccg atgcggtgat acttgccacg ggagcggtac tttcaaggaa
      121 catgaaaaaa gtcgttcagg aaacggctta cgtggtgagc atatgctcac tgccgcgact
      181 ttctgaccaa aagacaagaa agctgattga agaaaaccag gacaaaataa ctcttatatc
      241 gggtgatcgg gcaaagacac tggatcttgc cacggggctt aacagtggga aatttcttca
      301 gcaggaaatg aacgatgaaa cggaatatat atacatgccg ggagctttta caaaaagtgt
      361 gattgcagac atgggaaatg cggaactgaa aaaaacccat tttatactta aagatcccat
      421 gaaaatattc tttgatgcca taggctggca tcagctgatg aaaaagggaa caaaagtgtc
      481 ggtgctggaa aacataaagg tggcagccgt tactgtgaac cccgtttcgc cggcaggata
      541 ttctttcgga catgaagatc tgagagaagc cgtcagcaag gctctgcctg aaataccggt
      601 tattgatgta aggctttaaa caaggatatc atttagtccc ggaggataaa agtgataaaa
      661 agactggcaa atgtaaaaac cagaacaaat acaggcttcg atcatgtcat gaacggaatg
      721 gaactgcaga ctcccttcgg aacaaaaagg ctaaaagaac agaaaccttt tttcccgggg
      781 gaagaagctt ccttgagaag agaactggac aggatcggga gtctctttga tattgcccgt
      841 gaagatgaaa aatacatatc aaagatatcc gatatattta tggatatgaa ggatgtggag
      901 tttacagtcg aacgtagcaa aagcagcata ctgactgtgg tggagatctt cgagatgaaa
      961 tgcctgctcc ttaaaatgga gcagctgtct tctctttttc aaaaacagag caaaaagata
     1021 cccgacgaat tcatattaag cgatataagg atccttcttg atgaattgga tcccagagga
     1081 gacagactaa acacatttta tatttatgag gaattttcca aaaagctggc ggaactgcgc
     1141 aatgaaaaaa ggatgctgga aacaggggtc agaaaaagcc agaaggaaag aagagaaaaa
     1201 cttcacagag aaaagggcat aatgctgact ccaaaatttg actgtgtggt ctcaaaggct
     1261 gatgaagagg ccatcaggac attcgaggcg gctcctgaac tggagagatc atgccaggat
     1321 ataatttctg tgacattcca gctggtaagg acagaaaaag aaatagaaac gattgaaaaa
     1381 acagaaatgc tcactgctca gatagaagaa gaagaagaga aaataagaga aaagatttcc
     1441 gggagagtat gggcgtttca aaaagaaata cgggaaaact gcattcgcat cggatctttc
     1501 gatctgacac tggcgaaagc actttatgca ataaggcata gatgtgtaaa gccggaaata
     1561 ataaaagagt atgcggtatc cataaaagac ggcagacagc tccaggccga agaaatactt
     1621 gattccaagg gcaaggagta tatgcctgta agcataagtc ttgatccggg tgtgacatgc
     1681 ataaccggag ccaacatggg agggaaaaca gtttccctga aaatgatagg actcacggta
     1741 ctgctggccc agtacggtta ttttgtacca tgcaagagtg caaaaatcgg tctttcaaat
     1801 tttatgcaga tgctaatggg agacagtcag tctcttgaaa gaggcctttc aagtttcgga
     1861 tcggaaatgg aagagctgaa ggagatattc gaccattgca cggagagatc ccttatcatg
     1921 gtggatgaga tagccagcgg aacaaatccc tctgagggac ttgcacttac aaaaagcata
     1981 atagactttc ttaaggacga actttatata tctgtgatca ccactcattt cgatgtggga
     2041 gaaggcggcg gcaaagtaaa aaacgtgcag gtcacaggcc tggccaatgc cgacttcacc
     2101 agactggatc gggaacttag atatgcaaac cgtaaagagc ggataagcat aatcggaaag
     2161 tatatggatt acagactgtc accggcaaaa gaaggtaaaa cgataccaaa agatgctatc
     2221 aatatagtga aaatgctggg tatcgaaaat gaaacaatag aaaatgcaac aaaatactta
     2281 aaaggagaaa aaggatgaaa agcaaactga atctggaccc gaaagtaatc gacagcgcac
     2341 gttcctgtgc ttcaaggatc gctcacagca tgcaggaatt catcgatgtg catactactg
     2401 taagcacgga aagaactgtg gtgagacttt tgggcgtaga tggggtagat gatgtagaaa
     2461 caccccttcc aaacatcgtt atcgatcaga tacttgaagc aggaggactg ccaagaggtg
     2521 cggcatactg gatgggcaat gccatgatcc agaccgggct cgattctcag gagatagctg
     2581 aaaaaatttc tgcgggagaa ttaaacataa cagaacttcc cacggcgggc atcgaagagg
     2641 caaaagagaa aataatgcca catgtcaaag attctctcga caaaataaag gctcagacag
     2701 caaagagaga agagtatctg gccacaatag gtgagggaag gactccttac ctttatgtta
     2761 tcgttgcaac gggaaacata tatgaagacg tggtgcaggc caaggcggca gtaagacagg
     2821 gagcagacat agttgccgtt ataaggacaa ctgcccagag ccttttggac tatgtgccct
     2881 acggacccac aacggaaggc ttcggcggca cttatgccac ccaggaaaac ttcaggataa
     2941 tgagaaaagc ccttgacgaa gtcggtgaag aagtgggcaa gtatatcagg ctctgtaatt
     3001 attcttccgg catgtgcatg gctgagatcg cggccatggg agctcttgaa aggctggatg
     3061 taatgctcaa tgacgctctt tacggaatac ttttcagaga cataaatatg cagaggacac
     3121 ttattgacca gtatttctcc agggtaataa taggatactg cggaaccatg ttcaactcgg
     3181 gagaagacaa ttaccttacg actgatgacg catatgaagc cgcccatacc gtactggctt
     3241 cccagctgat caacgagcag tttgcagtac tggcaaatat acccgaagaa cagatgggtc
     3301 ttggtcatgc cttcgagatg gatccggata tcgaaaacgg attcctttat gaactggctc
     3361 aggcggtcat ggcaagggag atattcccca atgcgcccct caaatacatg cctccaacaa
     3421 aatacatgac aggcaatata ttcaagggcc atatccagga tgctcttttc aatatgatag
     3481 ccgtatggtc gggacagtcc ctgcagcttc tgggaatgcc cacggaagcc atacacaccc
     3541 cccacatgca tgacagatat ctttccatag aaaatgcttc atatatattc aacaatgcca
     3601 gagatattgg cgatgaagtt gaatttaagg aaaacggtat aatgcagcag agggctcaga
     3661 aagttcttaa ggaagccgat gaacttctcc atgagatcga aaaggacggt cttttctcaa
     3721 ctcttgaaaa aggaaaattc ggaggagtaa agagaccccg tgacggaggc aaaggactga
     3781 gcggcgtgtg cctcaaagac gaaaaatatt ttaacccgtt tattaccatt atgctgggag
     3841 gtgaaaaata atgactgata aaacagcagt agctcaggtc gacctgaaaa aagtcaagcc
     3901 ctacggggac acaatgaacg acggaatgac agaaacagcc ttcactctcc cggtgccttt
     3961 cggagaagaa ggaaatgagg cagcaaaaat acttgcgaag aaaatgggcc ttgatgagcc
     4021 taacgttgta tattcaaaag accagggcgg cggatacaca ttcttcatag tttacggaaa
     4081 gtgcagacat accgttgatt atacggaaat caaggtaaca aaagttgaag tcgaaataat
     4141 ggacaggatc gaatgtgcaa aattcatcga agaaaaaata aagagagatg tggttatcgt
     4201 aggtgcatcc acaggaacag acgcgcatac ggtgggaata gatgcgataa tgaatatgaa
     4261 gggatttcac ggacattttg gactggaaag atttgacggg atagaagcat acaattacgg
     4321 aagccaggtg cccaacgagg tactgatcga gaaggcaaaa gaagttaatg cggatgcaat
     4381 aataatttcc caaacagtca cacagaagga tatccacata gcaaatctga ctaatatggt
     4441 tgaacttctg gaagccgaag gtatgagaga caaggtcata ctttgctgcg gcggacccag
     4501 gatatcacat gagctggctc aggaactggg ctatgatgcc ggtttcggag cccatactta
     4561 tgccgagcat gtagcatcat ttatacttaa cgaaatcgtt gaaaaaggaa gagcataaga
     4621 aaggagagtt ttatgaaaaa taaaatcatg accgcaaaag aagcaatagc aggcatcaaa
     4681 gacggaagct ctataatggt aggtggtttt atgtgttgcg gaacaccgga gatacttatc
     4741 gatgctttag tggaaaaggg gacaaaagac cttactatca tatgtaatga cgcaggagta
     4801 ccgggaaggg gagtaggtaa gcttatcaca aacggacagg taaaaaccct tatagcttcc
     4861 catgtgggac ttaacccgga agtagctcaa agaatgaaca ccgaggatcc cgacgagaaa
     4921 ttggaatgca tccttgtacc ccaggggacc cttgccgaaa ggataagagc ctgcggagca
     4981 ggtctgggag gttttctgac tcccacaggg gtaggcacaa tagttgccga tggcaagaaa
     5041 gtaataaatg tgggaggaaa agactatttg ctggaggaac ccctgaaagc tgactttgca
     5101 tttttacgcg gttcggtcac ggataaattc ggaaatacca cttataatgc gacgaccaga
     5161 actttcaatc caatgatggc tgcagcggca gactatgtta tagtgggtgc ctgtgagatc
     5221 gttgagatgg gagaaatcga tccgaacaat attgtgacct cgggcatatt tgttgatgcg
     5281 atcgtaggag gtgagaaaca atggcagatc taaaggcaag gatagctacc cgtgtagcca
     5341 gagagttaaa agacggagat gttgtaaatc ttggaatagg acttcctaca aaagttgcaa
     5401 attatctccc cggcgatgtg cacataattc tccagtcgga gaacggaata atgggcatgg
     5461 gtcctgctgc cgaagaagga aaagaagacg ttgatataat caatgcagga gcccagtatg
     5521 tgaccattaa taccggggcc atgtactttg acagtgccac ttccttcggt atcataagag
     5581 gcggtcatgt ggatgccaca gtactgggag ccctgcaggt ggacaagcac ggcaatctgg
     5641 caaactggat agtccccggc aaaatggtgt ccggaatggg cggagcgatg gacctggtag
     5701 taggagcaaa aaaagtaata atatccatgc tgcatactca aaaaggcgca cacaaaatcc
     5761 tggatgaatg tactcttccc tatacagcga tcaaggtagt agatatgatc gtaacggaaa
     5821 tgggcgtaat ggaagttacc gatgaaggga tagtattaaa agaactgcac ccggacttta
     5881 ctctggagga gataaaggaa gccaccggat gccggctcat aataagcccg gatctcaagc
     5941 ccatggaaga ctagaggtca tataaaaaag aatagcgata atacccgccg cataaacaaa
     6001 agtgacgcaa ctgtaacaat tttctgaata attaccagaa cttgacattt tttaaatatg
     6061 tggtagactt taaacataaa gtgtgcttca atcggcacac ttttgttttg ggtgaaagcc
     6121 cattatggtg gcaagatgaa aaggagaaca gaatgcttgg cagcttattg aagcgcagta
     6181 atttgaaata ctgcgcccga gcgtttcttc taattatcag cattgctatg atagccggaa
     6241 caacagcact ggcagctgat gaaccggcag gaaaaggaca tgtggaagtc agtatcgccg
     6301 tggacgatat ggagatccat gcgaaaacag cacagatgac agtaagtgag gtcattgatt
     6361 cgcaggatat ccctcttggc ccggatgata tggtgacacc cgcagaagac accgtcgtag
     6421 aggacggaac gaaaatcgtg atcaaaagga tcctgaggaa agaaatgacc gtaacggaga
     6481 ctgttgatta caggacaatc aaaaagcagg actcggatct tgcgtacaac gaaaaaaaag
     6541 ttgtgcagga aggcagcgaa ggccttgaca gactgacata tgaggttttg tattccaggg
     6601 gcaaagaagt atccaaaaaa gaaatttcaa ggaccaccat aaaaaagccc agaaacagga
     6661 taatcaaaac aggctacaaa gataagtttg aggtcagagc atttgcctat accggcggcg
     6721 gtactaccgc cacaggaaca agggccagag tcggagcaat agctgttgat cccgatgtta
     6781 tacctttggg aaccaaagtg tacgtcgagg gttacggatc cgccgtggcg gaagacacag
     6841 gtggagcatt tatacagggc aaatcaatag acctgtatat gaactccaca gctgcatgtt
     6901 acagctgggg caagagatat gttactatat atatcttgta aaaagaataa gaaagaagaa
     6961 acgaaaagag ccgcatatgc ggctctttca tttttagcga aggtgctttt cagcaagatc
     7021 aataaacaga tccatatctt cttctttggg gaatatggcc cttgaggagg tggcattgcc
     7081 gcctcctttt ccgttgtata tgccggcatt ttctctcacc agcttactgc agtccacact
     7141 gccgtcggaa aagaatagaa gggtattatc tttttgcgaa actataacaa gaagtttttt
     7201 taccctgtct gaaagaggcc ttcctatgtt cagaaggtca tcggttttca tatcgctcca
     7261 tctgcagaca aggatgtcgc ctctgacgga aaaaggacct ttacaaaggg ggtcctcacc
     7321 ctttgtcagc ttgtccagga tctcagctgt ttcttcgatg cgggatgtta ttactgaatg
     7381 gcgaagtgta ttaagttcat ccctcataac tttgacacgg gtgtctgctg ctgcaatttt
     7441 tcccataagg tcttctgtgc ccgcggaatg tttgatgcaa agatcattta ctatactgtg
     7501 cttcttattg taatcagcca ttgctctggc gccggcctca aaatatatgc ggaacattcc
     7561 cttgtatttc tcaaccttgt atattttgac aaggcccacc tccgaggagg aatgaggatg
     7621 tgtgccgcag caggcaacac agtcggaagg gttatctttt tctcctatga gaaccacact
     7681 tacatcttcg tcaaaatcag cgggttttct cagaggaaga ctgttggcat cttcaggatc
     7741 ggtgaaaaga tgtatcgata tatccttgtt ttgccatatt atttcatttg caagatcctg
     7801 ggccctcatg gctatctccc agtcgatctc atttattttc atgccggagt tttgtgcatt
     7861 gttctcattg tcattttctt tttcgtcatc ctttttttca aggcttatat ctattgtcat
     7921 ataatcactg cccatgtgaa aaccccgatt gacacctccg tactcccttg agaaaactcc
     7981 ggacaggata tgttctccgc agtgacgctg catattgtcg aaccgtcttg cccagtcaag
     8041 ccggcaaagg acactctgcc ccttggaaaa taaggtttct gatttttcaa ggacatgaaa
     8101 tatttcattg tctttttcaa aaacatcccg aagaggaaca ccttctgttt ctccaaggtc
     8161 gcaggactgt cctccgccgc cgggaaaaaa gatggtagat ccaagaacca aaaccggata
     8221 tccgccttca gaaaaaacat ctgttatgac agattgatgt tcctttttat atacatcatc
     8281 caagaatatt ttttttgttc catttttttg ttccattttt ttctcctcta taaaaagtat
     8341 tatagcacat aaaccggaaa gcagagaaaa aaggggtatc cgccatgtaa ttgattattt
     8401 cggcaaatac agatttttat atatcatgag cagatataac ttcttgatta actgaggaca
     8461 taaaaagaaa aacaaatata cataaatttt agtttatatg gtatataaca aatacaatca
     8521 gcataaagca tttgtgatat aataaaaaaa gcgaaaaacc ataaatcaca aatcaataag
     8581 ccacaaatca acaaatcaat aactaagcaa accgataaga aaataaaccg gcaagttcgg
     8641 aaaacggata catataacgg agaggtgaac atgaaatact cactggatgt caaaacaagt
     8701 tctcataccc aaatgcttga tataacaaaa gacattgaaa aaatcgtaaa agaaagcaaa
     8761 atcagaaacg gaacatgcac ggtattcatt ccgcatacaa cagctgctgt aactataaat
     8821 gaaaatgcag atcccgatgt ccaaaaagac tttataaaga cgataaacaa aattgtgcca
     8881 tgggaggacg attatcttca tatggaaggt aactccgctg cacatctcaa agcaagtatg
     8941 atagggtttt cagagcagat aataatagaa gaaggaaggc ttttgctggg cacatggcag
     9001 gggatatatt ttatagaatt tgacggtccc agaaacagaa aggtgtgggt cggaatcctt
     9061 gcggatcaga agagcgcaaa tgggaggcaa gaaacaactg atgggtcaga ataaaaaaca
     9121 gcaagaagca acatagtaca agtgataggg tgaacacaaa atgttgtgcg cataaagaag
     9181 agagaaatcc tgtaaatgcg aataacacgt gtaacagtcc ggatacgcat aatcagacaa
     9241 ttttgaaaaa acaatatatt gtgcgtgcta aaaaataata attgttgtat aataaaaaaa
     9301 catagagtac taatttgcgg tatgaagcag tgcgaacaaa aacaaacggg tgaaataaaa
     9361 agagaacaac ctgtggataa agaaaaggaa aaaggtgtag aaaacaataa taagccttgg
     9421 aaacactaac ttatgcaaaa aatagttttc cacaggacac ggtgcataaa attgtatacg
     9481 atttggtata tgctgtggac aaacatcaaa aaacaagaaa aacagacact ttcggcttgt
     9541 tgatattata agcccggaag tgcgaacaaa ggttgacata aaacatcggg aacggaaaca
     9601 aaaatgttaa aagagagaat cacattcaaa agcgagctgc cggtaaatgt gctggtagcg
     9661 gacatagaag aatatcccat acatttccat gatgagctgg aaatggtatt tgttcttaaa
     9721 ggacatgtaa agctgagaaa cggatattat aattacgatt tgaagcaggg agacattttt
     9781 actttgaacg acagagagat acatagcttc agcaggatag aaaaccagga aaacatggtc
     9841 atgctactgc agatggatct tacttatttc tcccgctact ataataatct gaaaaacaat
     9901 tttttcgtta cggacatgga agatgatgac gatgagagtc tggatatact gagaaagttc
     9961 ctggccaaga taatgatgga tatactggag aagggtttcg gatacgaaca gaaagttata
    10021 gaaagtaccc acaaccttat tgccagtctt caggccgatt ttcagtattt tgtcatggaa
    10081 gacggacgtt tcgtgaacga agtaaaaaag aagggcaaca aaatcctcgc aggtcgtctt
    10141 aacaggataa ccgactacat gtacgacaat tattacagaa aactgacact caaagaaata
    10201 gcagaaaaag aacacctctc catttattac ctttcccatg tcataaaaga ggcaacgggt
    10261 ctcagttttc aggatcttct caatttcatc agagtagaag aatcggagaa actgcttttg
    10321 ggaaccaata agcgtatagg agccattgca gaagaaacag ggttttcagc tgtccgctac
    10381 tatatcaaac actttgagac atggttccac atgcatccgg cagaatacag aaaaaaattc
    10441 acaggcaaag tcagcggcag agaaagcgga gcaaaaatga gaagggctgc gccggctgaa
    10501 atcgaagagg cacttaaaca gcaggtaaaa gggctttaca gagactactt cttcaggaac
    10561 gcaataaagc ccataatagt tgatcttgat atagacaaag aagtatccgg cacctttgga
    10621 aagagtgtga tggatgagct tatgcaaagg gatatcatga agcaggtttc ggctccctat
    10681 ataatgatga aaaacctggg agaggaaatt atagcatcgg aagacaatta tctgatctca
    10741 gcagataaag atgaagcagg aaaaataaat gccctttcca tacttatata taatttcagc
    10801 gaagaccttg gcagggaact tggcatggtt tcatcaaagg aggaacttat aaacagcctt
    10861 tctgtttatg aggcggaggg agagatactg gtaagatgtg ccggcatgac cggagatttc
    10921 aggatatcca gataccgcat atccagacaa aactgtctta tggcaatcga cagttcggga
    10981 atacatgaca aaagtcattc tgcaagagag atcttaagag ggaagctggc tgccatgccg
    11041 gttgttgaat ttgaagagtt tcatgttgcc gataccataa gcgtaagatc caccataaag
    11101 ggctttggag cagaactgat tcttatcgac aagaaaaccg caaaatagta cacaataaga
    11161 gcaaatgcgt gattgtattt tatcaccggg gtgataaaat aaaagcacaa aggaaaatgt
    11221 gcacttattg aagtgttcaa ataatggagg taaaaatatg aaaattctta ttatcggagc
    11281 aggcggcgtg ggcacatcca ttgccaccat catcaaaaga atgggcaaag aggttgactg
    11341 ggcagaaaaa gtcgttctgg cagattacaa ctttgacaga gccaaggaag tggcagacaa
    11401 gatctgcggc ggcggaatat ttgttgcaga aaaggtgaat gcaagagatt ctgcaaatat
    11461 cgaagaaata atcaagaagc atgagatcga tattactatg aacgcagtag aacctgagtt
    11521 caatgaatgt atatttgaca catgccttaa aacaggatcc aaatatatgg acgctgcaat
    11581 gacactttcc acaccggatc ccgacaaacc cttcgaaaag gcaaacatca aactgggaga
    11641 ctatcagttc gacaggcatg aagactggaa gaaagccggt aacatggcac ttctgggaat
    11701 gggtgttgag cccggaatgg cagacgtatt tgccaggttt gcagaaaaac atctttttga
    11761 cgagatcgac caggttaacg ttcgtgacgg agataactat gacggcgaag atgtgggcta
    11821 cttcggattc tcagtatgga ccacaataga agagtgtctc aatcctcctg ttgtatggga
    11881 aaagggcaag ggcttcttct gcacagagaa ctttccgaac ctgaaatgtt caatttcccc
    11941 ggaggcatag gcgatgttga agtaataaat gttgagcatg aagaagttct tctggtaccc
    12001 agagtaatag actgtaacag agtcacattc aaatatggtg ttcccaaaga aacaagagac
    12061 atgcttcttt atctgaagga cctgggtctt tccgaaaaga caaagaaaat aaaagtcggc
    12121 gacagtgaaa taacacccag agatttcctg gtaaaagtaa taccgagtcc tgtggaagcc
    12181 accatgagaa tggaaggcaa aggctgtgca ggtacatggg tcacaggtac aaaagacgga
    12241 aagagaagat cggtctatct gtatcaggta gctgacaatc aggactgcat caaaaagtac
    12301 acaacaaatt cagttgttgc ccagactgca gtaggtcctg ttattgctct tgaacttata
    12361 gcaaaaggca tctggaatct ggaaggcgta tacggtcccg aaagctttga tccggatccg
    12421 tttgtagaaa gacttgccaa gtatgagttc ccggcgggaa taagagaaat ggattcagaa
    12481 tatgcagatt tcctgaatga aaaagcattc gaagaagctg taaaatagga tctgttaaaa
    12541 ggacgccgtt tcataaattg aaaagcggca aaagcccaag aaaatgacaa agcaaacagc
    12601 caccttttgg tggctgtttt tgtttaatag ggcgtttaag cccgtgtcga gtgcgtgggc
    12661 gcacgagata aaaacca
//
LOCUS       DAONHK010000021        12582 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_3931_length_12582_cov_5.085016, whole genome shotgun sequence.
ACCESSION   DAONHK010000021 DAONHK010000000
VERSION     DAONHK010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 12582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12582
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3931_length_12582_cov_5.085016"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1061
                     /gene="recF"
                     /locus_tag="PLM92_01540"
     CDS             <1..1061
                     /gene="recF"
                     /locus_tag="PLM92_01540"
                     /inference="COORDINATES: protein motif:HMM:NF000823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HPR23855.1"
                     /translation="KEKVSFHKKVNMILGENAQGKTNLLESIYLLSLGKSFRTPREKE
                     MIKMGSEFCRVKTVYKEKGDEEETLEIFISKEGKKGVKKNSLKTEKISQLLDNIYVVV
                     FSPEDLKIVKEDPEKRRIFMDREMCQMKYSYMSSLYSYKKILAQRNAYLKEKNTDENI
                     MEVWEEQLSEAGAVLISGREEFIKKTDEISRDLHSEITEGKEKLEIIYRPSIENCENK
                     EELKKNIKKTLEKNRKKDLFYGNTGKGPHKDDFDIYINGNEAKKYGSQGQQRTAALSI
                     KLAETKIIEKEKKEKPILLLDDVLSELDANRQKYLINSLWDMQLFITTTEINPDIKKT
                     LPEGKEYRIENGKVLQKA"
     gene            1228..3168
                     /gene="gyrB"
                     /locus_tag="PLM92_01545"
     CDS             1228..3168
                     /gene="gyrB"
                     /locus_tag="PLM92_01545"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HPR23856.1"
                     /translation="MAANTKQQKYDAEQIQVLEGLEPVRKRPGMYIGSTGEKGLHHLV
                     YEIVDNSIDEALAGFCNNINVTIQKDDSIVVEDDGRGMPVDMHPKLKISALEVIHTVL
                     HAGGKFGGGGYKVSGGLHGVGASVVNALSTKMEVEIKRNGKIYYQEYSKGKAVTRLTE
                     KGTSKKTGSRTAFWPDPDIFEEIDFDFATLEYRLREMAFLNKGVSITLKDEREGLKRK
                     EVFFYEGGIKEFVKYQNQSKEPIHPEVIYFEAKAKDKTGKGDMEVEVAMQYTDRYNEV
                     ILSYANNINTTDGGFHLIGYKTALTRAFNDYARKNKFLKENDQPLTGEDVREGLTSII
                     SVKLTSPQFEGQTKAKLGNSEMRGFVETATNENLTAFLQENPNQAKIIIAKCLQAARA
                     REAARKAREITRKNSILDGLALLGKLADCSEKDPAFSEIFLVEGDSAGGSAKQGRDRK
                     RQAVLPLRGKILNVEKARLEKILNSDEIKNMITAFGCSIGQEFDVQKLRYHKIIIMTD
                     ADVDGAHIRTLLLTFFYRYMTPLIEGGYVYAAQPPLFRIKKGKDTYYTYSEREQMKVL
                     ASLTDKPGKVDIQRYKGLGEMDFQQLWETTMDYKKRTLIQITLEDTAAADAIFTTLMG
                     DKVPPRKKFIEDNAKYVQNLDI"
     gene            3179..5701
                     /gene="gyrA"
                     /locus_tag="PLM92_01550"
     CDS             3179..5701
                     /gene="gyrA"
                     /locus_tag="PLM92_01550"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005999122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HPR23857.1"
                     /translation="MTNLLEDQKLIQHEIHSEMKNSYIDYAMSVIVGRALPDVRDGMK
                     PVHRRILYGMSELGVTPDKPHKKSARIVGEVMGKYHPHGDSSIYDAMVRMAQDFSIRY
                     MMVDGHGNFGSVDGDGAAAMRYTEARMTPFALQMLKDIDKETVEFVPNFDEEEKEPVV
                     LPARFPNLLVNGSNGIAVGMATSIPPHNLGEVIDAAIKMIDEPDCSIKDLTKCVKGPD
                     FPTGGIIMGKSGMREAYNNGQGRVKVRALVETEETARGRYQIIVKEIPFQVNKSKLIE
                     KIAELVKEKKIEGISYIRDESNRDGMRIVIELKKDANPQITLNRLYKHTQMEDSYSMI
                     MIALVDGQPRILNLQEILFEYLKHQKDVITRRTKYDLAKAEARAHILEGLRIALDNID
                     AIIKIIRTSYDDAKERLMKEYRLSDIQAQAILDMRLARLQGLEREKIENEYAELQKRI
                     KYYKEILSDENLLMGVIKDEMLEIKQKYADKRRTKIMADVAEMDEEDLIEEQQVTITL
                     THLGYLKRIPTDTYKAQRRGGKGIMGVTTREKDFVTDLIMTSTHDTLMFFTNLGKAHK
                     IKAYEIPEAGRTAKGTPAVNFLSLLQRERINAIIPIKEFSEERYLIGVTKHGTIKKTA
                     LSNFDTNRKAGLIAINLKDGDELVGIKQTSGTDNVIIVTKHGKCICFSENDVRPMGRI
                     AGGVRAIKLEKDDEVVAMQLVQPKEQLLVVTKKGFGKRTAAKEYKVQVRGGKGLLTYD
                     KRKFKKTGELIGAMVVDDEDEVLLINSNGIIIRIKAGDVSRLGRATQGVKIMKVSDDT
                     EIISMAKVIKEEEITLEKKENGSKEKDHGQLSMETLGGEKKK"
     gene            5725..6147
                     /locus_tag="PLM92_01555"
     CDS             5725..6147
                     /locus_tag="PLM92_01555"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPR23858.1"
                     /translation="MIEKIKMTMPGNPKYTVVTELAVEAAAVQAGFDLEAVNDIKLAT
                     GEACKQVICHGYEGWCSCYEVECETDGEKIVIKIFETDCGHKVPKKGKICLDCPREGD
                     LSLEIIKTLMDETEIKKTGSGEKGKKSIIMIKKKSHNK"
     gene            6177..6920
                     /locus_tag="PLM92_01560"
     CDS             6177..6920
                     /locus_tag="PLM92_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005999118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPR23859.1"
                     /translation="MDKKEMFRKYRATGDKEIRNRIVEEHLYMVDILIRKYLNKGMEY
                     DDLYQVGAMALINAVERFDPEKGFEFSSFATPTILGEIKKHFRDTGWSMKVPRKLKEN
                     ASAIPAAKDALGLKLGRTPTVKELAEEMNIGEDELLEAMQGSLAYGTFSLDQTFEEDD
                     KNGDNPVFEKYAYQEEKGFDRFENREIIGSVLKKLNSTNQYVFKKRFLEERSQAEIAK
                     KLGVSQMTISRAEKNIKEKFAEELQKALN"
     gene            6997..7977
                     /gene="trpS"
                     /locus_tag="PLM92_01565"
     CDS             6997..7977
                     /gene="trpS"
                     /locus_tag="PLM92_01565"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006000407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HPR23860.1"
                     /translation="MKRVFSGVQPTGNIHIGNYLGALKQFVELQKENQCIYCIVDMHS
                     ITFPQDPKQLKKHILDVAALYMAVGIDTKKSIVFVQSDVPGHAELAWILACNSYTGEL
                     SRMTQFKQKNKGRESAPTGLFTYPILMAADILLYDTDIVPVGNDQKQHIELCRDIAIR
                     INNKYKKTFIVPDGRFLKAGARIMALDDPLCKMSKSAENIHSRISLLDDEGKIKKSIM
                     RATTDSEGSIRFDMKEKPGISNLMNIYSAFSGMETSEVEKAFGGKGYGDFKKELVQVT
                     KEALTPIKEKYEEIRPSKELLDILKDGAERANAISEKTIKRVKENFGLGL"
     gene            complement(8019..8759)
                     /locus_tag="PLM92_01570"
     CDS             complement(8019..8759)
                     /locus_tag="PLM92_01570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPR23861.1"
                     /translation="MKIIRNIPQVIKIYRSLRYLNEYKKEIDSGRFSGDMKKEKENIL
                     KATKTWGSKLSEMIGVEINVYGTENLPKDSPVVYVSNHQGYADIVVLCAVLDTVQFGF
                     IAKNGLEKVPLYGTWIKRIRSIMIERDDPRESLKAISRGIDYINLGFSLLIFPEGTRS
                     RGPVMGEFKKGSLKLATKPQVSIVPVSVNNTWHIFEETGIVTPHPVSVMIHPAIETCA
                     LDKEQEKALTEKVRAVIKNGLDELQKKL"
     gene            8876..9124
                     /locus_tag="PLM92_01575"
     CDS             8876..9124
                     /locus_tag="PLM92_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPR23862.1"
                     /translation="MTTKKKKTGKYAAAVLCAVAIILYEAAVVYGVFKLGLDEKIPVW
                     AVVLIITIPALAAAGIIAALILRIKEIKGGEEDVASKY"
     gene            9105..9419
                     /locus_tag="PLM92_01580"
     CDS             9105..9419
                     /locus_tag="PLM92_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019163048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HPR23863.1"
                     /translation="MLLLSINYIPDQEIEALGIVKGNVVQSKNVGRDIMAGMKGLVGG
                     ELSGYTDMLNQARQIATKRMVDEAEAMGADAIIRVEYASSAIMQGAAEIIAYGTAVKF
                     KK"
     gene            9416..10063
                     /locus_tag="PLM92_01585"
     CDS             9416..10063
                     /locus_tag="PLM92_01585"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HPR23864.1"
                     /translation="MTKKTKTLQVAILSGAVVCFALITYTVTTGISSGFDNAVMEFFY
                     GIRKPGLDTLMELITYMANWETIVIICLLLVCFPQTRKKAGISVSIAALLSTLIKAVI
                     KTAVARPRPEESLFLIEQSGSAYPSGHAMTGMAVYLLLFLFTREYIKDSRKKNLLSFV
                     FVFAAFAVGISRVYLGVHFPTDVLGGWTLGFATVILSLIIWEILKKRLPYCSDLS"
     gene            complement(10042..10578)
                     /locus_tag="PLM92_01590"
     CDS             complement(10042..10578)
                     /locus_tag="PLM92_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007050537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2148 domain-containing protein"
                     /protein_id="HPR23865.1"
                     /translation="MTIKSGDAERKAAFAVADLMAAAVRTAPKGCGVDEIETFIIEGK
                     DKDLLAGHMKDIAKETGEDFYERDGKNVDNSHCVVLIAVKNEPLGLTNCSLCGFPDCA
                     AMEKAGANCAFKVTDLGIAIGSAVSVAADHRIDNRVLFSAGKGAMRMDLFSEKVRVCF
                     GIPLGVNGKSLFYDRSEQ"
     gene            10715..>12582
                     /locus_tag="PLM92_01595"
     CDS             10715..>12582
                     /locus_tag="PLM92_01595"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HPR23866.1"
                     /translation="MRDGYNIKKSRWLTAGSIGAVVCILIIVFSYYSYEKKLDENLKD
                     YAYSKLDETVKDQAFVISSKIHDESRILVNIADYLAKEGTDTKKNLLMASMEKGSTFE
                     AIGYSDKNGIITEPDGKQTDISKREYFEKAIKGEETTQYVEKSIEEGKPEIIIAVPVK
                     DGSEIKGVVAGKFGTDQVEKALKTEDFGGKGYFCLCNSKGTVVAATKRKSCIFNNAEN
                     IFEKYENMQITGGDTIETVKEKIKAKKSGYIEFSYKNRSYYTRYVAKAPSWILLYTAP
                     TEVIEENMSFMKKGTVELVIGLMIAMAVIFGALFYEQTRATRRYKQINHEMNNINESL
                     DIALSQTPRMILDYDKKTDTAKVFKNYMDEKAYPIGHVFGEFSESDGFVYVEDKEIHK
                     RIIKRTEQDERADDGYIQEYVRYTFDGGSTFIWCYVTGQRKLDEQGNIEGAIFVLEDV
                     NVDKEELKNIEKKAETDLLTGLLNKKFTEKKVTELIGPKKTEGFLFMMDLDDFKRVND
                     EKGHAIGDIILEKTADAVRRSFREEDIKGRVGGDEFLVFMPGTTSGKGAADRANKIAE
                     ELKKISEYVPGTDITCSIGIASYPKDGRTFRELYEAVDKAMYHVKRHGKKGYSFTKG"
BASE COUNT         4591 a         2227 c         2859 g         2905 t
ORIGIN      
        1 aaaaagaaaa ggtgagcttt cataaaaaag taaatatgat ccttggagaa aatgcccagg
       61 gaaaaacaaa tcttcttgaa agcatatatc tgttgtcttt gggaaaatcc ttccgcacac
      121 caagagaaaa agaaatgata aaaatgggtt cggaattttg tcgtgtaaaa accgtttata
      181 aagaaaaagg tgatgaagaa gaaactcttg aaatattcat aagcaaagaa ggaaaaaaag
      241 gagtaaaaaa aaacagtctt aaaacagaaa aaatatctca gctccttgac aacatatatg
      301 ttgttgtttt ttctccggaa gatctgaaaa tagtaaaaga agatcctgag aaaagaagaa
      361 tcttcatgga cagagaaatg tgtcagatga aatactcata tatgagcagt ttatactcat
      421 ataaaaagat actggcacag agaaatgctt atctgaagga aaaaaataca gatgaaaaca
      481 taatggaagt ttgggaagag cagctttcgg aagccggagc ggttttgatt tccggaaggg
      541 aagagtttat aaaaaaaaca gacgaaataa gcagggattt gcattctgaa ataacagaag
      601 gaaaagagaa acttgaaata atatacagac ccagtattga aaactgtgaa aataaagaag
      661 aactgaaaaa aaatataaaa aagacactgg aaaaaaacag aaaaaaagac cttttttacg
      721 gaaatacggg aaaaggaccg cataaagacg attttgatat ttatataaac gggaatgaag
      781 caaaaaagta tggatctcag ggacagcaaa gaacagcggc gctttccatt aaacttgccg
      841 aaacaaaaat tattgaaaaa gaaaaaaaag aaaagcctat acttcttttg gacgatgtcc
      901 tttctgagct tgatgcaaac agacaaaagt accttataaa ttccctttgg gatatgcagc
      961 tttttataac gaccaccgaa ataaacccgg acattaaaaa gactttgccg gagggaaaag
     1021 aatacaggat agaaaatgga aaagttttac aaaaagcata aacacatata agcacctgaa
     1081 aaggcgcctg ttcataaaaa actaactttt gaagcattta aaaaaactta attaacaaaa
     1141 agatcaaaca gcttattttc attgtaaaaa gggctgtttt gttatataat aaaaggcgat
     1201 aagaaagaca aagaggtgat ttttttaatg gcagcaaaca caaaacagca aaaatatgac
     1261 gcggagcaga tccaagttct ggaaggcctg gaacctgtaa gaaaaagacc cggtatgtat
     1321 atcgggagta caggggaaaa aggtcttcat catttggttt atgagatagt tgataacagc
     1381 atagacgaag ctctggcagg cttttgcaac aatataaatg ttactataca gaaagatgac
     1441 tccatagtag ttgaagatga cgggcgggga atgcccgttg atatgcaccc caaattaaaa
     1501 atatcggcac ttgaggtaat acatacagtt cttcatgccg ggggaaaatt cggcggcggc
     1561 ggatacaagg tttccggagg acttcacgga gttggtgctt cggtggtcaa tgctctttcc
     1621 accaaaatgg aagtagagat aaaaagaaac ggaaaaatat actatcagga atattccaag
     1681 ggcaaagcag tgaccaggct tacggaaaaa ggaacatcaa aaaaaacggg atcaaggaca
     1741 gcattctggc cggatccgga tatattcgaa gagatagact ttgattttgc cactctggag
     1801 tacagactga gagaaatggc ctttcttaac aaaggtgtaa gcataaccct taaggatgaa
     1861 agagaagggc tgaaaagaaa agaagtcttt ttctatgagg gcggaataaa agaattcgta
     1921 aaataccaga atcaaagcaa agaaccgatc catcccgaag ttatttattt cgaggcaaag
     1981 gcaaaggaca agaccggcaa aggggatatg gaagttgaag tggccatgca gtatacagac
     2041 cgctataacg aagtcatcct ttcatatgca aacaatataa ataccacaga tggaggcttc
     2101 catctgatag gatataaaac ggctcttaca agagcgttta atgattatgc aagaaaaaac
     2161 aaattcctta aagaaaatga tcagcccctt acgggagaag acgtaaggga aggccttact
     2221 tccataattt ccgtaaaact tacaagcccc cagtttgaag gtcagacaaa ggcaaagttg
     2281 ggaaacagtg aaatgagagg atttgtggaa acggcaacca atgagaatct tacagcattt
     2341 cttcaggaaa atcccaacca ggcaaaaatc ataattgcaa agtgtcttca ggcggcaaga
     2401 gccagagaag cggcaagaaa agccagagaa ataaccagaa agaacagtat actggacgga
     2461 ctggcacttc tgggcaagct ggcagactgc tcagaaaaag atccggcctt ttcagaaata
     2521 ttccttgttg agggagattc tgcgggagga agcgcaaagc agggaagaga cagaaagcgt
     2581 caggcagtac ttcctttaag aggcaagata ctgaatgttg aaaaagccag gctcgaaaag
     2641 atcctaaact ccgatgaaat aaaaaatatg ataacggctt tcggatgtag catagggcag
     2701 gaattcgacg ttcagaaact ccgttatcac aagataataa taatgacaga tgccgatgtg
     2761 gacggagctc atataaggac actccttctg acattcttct acagatatat gacacctctt
     2821 atcgaaggcg ggtatgttta cgctgcccag cctccgcttt ttcgtataaa aaagggaaaa
     2881 gacacatatt acacctattc cgaaagggaa cagatgaaag ttcttgcttc tctgacggac
     2941 aagcccggaa aagtggacat acaaagatat aagggcctgg gagaaatgga ctttcagcag
     3001 ctttgggaaa ccacaatgga ttataaaaaa aggaccctta tccagataac actggaagat
     3061 acagcggcag cggatgctat ttttaccacc cttatgggag ataaagtacc gccaagaaag
     3121 aaatttatag aagataatgc caaatatgtt caaaaccttg acatttagga gataaaaaat
     3181 gacaaattta ctcgaagatc agaaacttat acagcacgag atccacagcg aaatgaaaaa
     3241 ctcctatatt gattatgcca tgagcgtaat agtgggacga gcccttcccg atgtgaggga
     3301 cggaatgaaa cccgtgcaca gaaggatact ttacggcatg agcgaactgg gagtgactcc
     3361 ggataaacca cacaaaaagt ccgcccgtat cgtgggagaa gtaatgggta aatatcatcc
     3421 tcacggtgat tcgtctattt acgatgcgat ggtaagaatg gcccaggact tttccataag
     3481 gtacatgatg gtggacggac acgggaattt cggttctgtg gacggtgacg gagctgcggc
     3541 gatgcgttac actgaggcga ggatgacccc gttcgcattg cagatgctaa aggatataga
     3601 taaagaaact gtagagtttg tacccaattt tgatgaagaa gaaaaagaac ccgtagtgct
     3661 tccggccaga tttcccaatc tgctggtaaa cggatcaaac ggtatagccg ttggaatggc
     3721 cacatcgata cctcctcata atcttggaga agtcatagat gcagcgataa aaatgatcga
     3781 cgaaccggat tgcagcataa aggatctcac aaaatgtgta aaaggaccgg attttcccac
     3841 cggcggcatc atcatgggaa aaagcggcat gagggaagct tataataacg gccagggaag
     3901 ggtcaaggtc cgcgctcttg tagaaacaga agaaacagcc agaggcagat atcagataat
     3961 agtaaaggaa atccctttcc aggtaaacaa gtcaaaactt atcgaaaaaa tcgctgaact
     4021 ggtaaaagaa aaaaagattg aaggcatatc atatataaga gatgaatcca acagagacgg
     4081 gatgcgtata gttatagaac tgaaaaaaga cgcaaatcca cagataacac tgaatagact
     4141 ttataaacac acgcagatgg aagacagtta cagcatgata atgatcgcgc tggtggatgg
     4201 tcagccgagg atactgaatc tgcaggaaat actttttgaa tatctgaaac accagaagga
     4261 cgtaataaca agaagaacaa aatacgatct tgccaaggcg gaagccagag cacatatact
     4321 ggaaggactg aggatagccc ttgataacat agatgcaata ataaaaataa taagaaccag
     4381 ctatgatgac gcaaaagaaa ggctgatgaa agaatacagg ctttcggaca tacaggccca
     4441 ggccattttg gacatgagac tggccaggct tcagggtctt gaaagagaaa aaatagaaaa
     4501 cgaatacgcg gaacttcaga agagaataaa atattacaaa gaaatacttt ccgatgaaaa
     4561 tcttttgatg ggtgtcataa aagatgaaat gctagagata aaacaaaagt atgccgacaa
     4621 aagaaggaca aagataatgg cggatgtggc agaaatggat gaagaggacc ttatcgaaga
     4681 acagcaggtc actattactc ttactcattt gggatatttg aagaggatac cgacagacac
     4741 atataaggct cagagaagag gcggaaaagg tataatgggc gtaacaacaa gggaaaagga
     4801 ttttgttaca gaccttataa tgacatctac tcatgatact cttatgttct ttacaaatct
     4861 ggggaaagca cataaaatca aggcttatga gatccctgaa gccggcagga cggcaaaggg
     4921 aacccctgcg gtgaacttcc tttccctgct tcagagagaa agaataaatg caataatacc
     4981 cataaaggag ttttcagaag agcggtatct tataggtgtc acaaaacacg ggaccatcaa
     5041 aaaaaccgca ttgtccaatt tcgataccaa cagaaaagcc ggtctgatcg ccatcaacct
     5101 caaagacggg gatgaactgg tgggaatcaa acagacttcc ggtaccgata atgttataat
     5161 cgtaacgaag cacggaaaat gcatatgctt ttcggaaaat gatgtaagac ccatgggcag
     5221 gatagcagga ggcgtacgtg cgataaaact tgaaaaagat gatgaagtcg tggcgatgca
     5281 actggtacag cccaaagagc agctccttgt ggtgacaaaa aaaggtttcg gcaaaagaac
     5341 tgccgcaaaa gaatacaaag ttcaggtaag aggcggcaag ggccttttga cttatgacaa
     5401 gaggaaattc aaaaaaaccg gagagcttat aggcgctatg gtcgtggatg atgaagatga
     5461 agtactcctt ataaattcca acggaataat aataaggata aaagcaggag atgtctcaag
     5521 gctgggtcgg gcgacccagg gggtcaagat aatgaaggtt tcagatgaca cagaaataat
     5581 atccatggcc aaggtcataa aagaagaaga aatcacttta gaaaaaaaag aaaacggcag
     5641 caaagaaaaa gaccatgggc agctatccat ggaaactctt ggtggagaga aaaagaaata
     5701 aaaacagtaa aaaaggagaa aaaaatgata gagaaaatca aaatgaccat gccgggcaat
     5761 ccaaaatata ccgtggttac agagctggcg gtggaagcgg ctgccgttca ggcgggattt
     5821 gacctggaag ccgtaaatga tataaaactt gccaccgggg aggcctgtaa gcaggtcata
     5881 tgccacggtt atgagggctg gtgttcctgt tatgaagttg agtgtgaaac agacggagag
     5941 aaaatagtaa tcaaaatatt tgaaaccgat tgcgggcata aggtaccaaa aaaaggcaag
     6001 atctgtcttg actgtcccag agagggagac cttagcctgg aaataataaa aacattgatg
     6061 gatgaaacag aaataaaaaa aaccggatcc ggcgaaaaag gaaagaaaag cataatcatg
     6121 atcaagaaaa aaagtcataa caaataagtt tcaagaagat acgcagaggc attaaaatgg
     6181 acaaaaaaga aatgttcagg aagtacagag caaccggaga caaggaaata agaaaccgta
     6241 tcgtcgagga gcatttgtac atggttgaca ttttaataag aaagtatttg aacaagggca
     6301 tggaatacga tgatctttat caggtgggag ccatggcgct tattaatgct gttgaaagat
     6361 tcgatcccga aaagggattt gaattcagca gttttgcaac gccaaccata ttgggagaga
     6421 taaaaaaaca ttttcgcgat acgggctgga gcatgaaagt gccaaggaaa ttaaaagaaa
     6481 atgcatcagc tatacctgcg gcaaaagatg ctcttggact aaaactggga cgcacaccca
     6541 cagtaaaaga actggcggaa gaaatgaaca taggagaaga tgagcttctt gaagctatgc
     6601 aaggaagcct ggcctacgga actttttctc tggaccagac atttgaagaa gacgataaaa
     6661 acggagacaa tccggttttt gaaaagtatg cctatcaaga agaaaaagga tttgacagat
     6721 ttgaaaacag agaaataatc ggatccgtgc tcaaaaaact aaacagcaca aaccaatatg
     6781 ttttcaaaaa aagatttctt gaagaaagat cccaggcgga gatagcaaaa aaactgggtg
     6841 tatctcaaat gaccatttca cgggcagaaa aaaacataaa agaaaaattt gcggaagagc
     6901 tgcaaaaggc cctcaactaa acagcagata gaagcagatc aaaaaataca taacaataca
     6961 catacagata tagcagacaa aagacggagg caatagatga aaagagtatt ttcgggcgtt
     7021 caacccacag gcaacataca cataggtaat tatctgggag cccttaaaca gttcgttgaa
     7081 cttcagaaag aaaaccagtg catatactgc atagtagata tgcattcaat aacatttcca
     7141 caggatccga aacaactgaa aaaacatata cttgatgtgg cagcccttta tatggccgtg
     7201 ggaatagata caaaaaaatc aattgttttc gtccagtctg acgttcccgg acatgcggag
     7261 ctggcctgga tactggcatg taattcttat acgggagaac tgtccagaat gacacagttc
     7321 aagcaaaaaa acaaaggaag agaatccgca cccacaggac tttttactta ccccatactt
     7381 atggctgcag acatacttct gtatgatact gatatagtcc ccgtcggtaa cgatcagaag
     7441 caacacatag aactatgcag agatattgcc ataaggataa acaacaaata taaaaaaact
     7501 tttatcgttc ccgacggacg ttttcttaag gcgggagcca ggataatggc gctggacgat
     7561 cctttatgta aaatgagcaa gagtgcagaa aacattcaca gcaggatatc actccttgat
     7621 gatgaaggga aaataaagaa atccataatg agggccacga cagactcgga aggaagcata
     7681 agattcgata tgaaagaaaa acccggaata agcaatctta tgaacatata cagtgcgttc
     7741 tcgggcatgg agacatctga agtagaaaaa gcctttggcg gaaagggtta cggcgacttc
     7801 aaaaaagaac tggttcaagt gacaaaagaa gcactgacac cgataaaaga aaaatatgaa
     7861 gagataagac cttcaaaaga gcttcttgat atactgaaag acggggcgga aagagcaaat
     7921 gccatttccg aaaaaacaat aaaaagagtg aaagaaaact ttggactcgg cctataggtc
     7981 gcaaaaaaca gtaaagcaac gcagtggccg gctaaaaatc ataatttttt ctgcagttca
     8041 tcaagaccat tttttataac agctctgact ttttcggtca gggctttttc ctgctccttg
     8101 tcaagagcgc aggtctctat ggccgggtgt atcattacgc ttaccggatg aggagttacg
     8161 ataccggttt cctcaaatat atgccaggtg ttgttaacgg aaacgggaac gatggaaacc
     8221 tgtggttttg tggccagctt caatgatcct tttttgaatt ctcccataac agggccccgg
     8281 cttctcgtac cttccggaaa aataagaaga gaaaaaccaa gatttatgta atcaatgccc
     8341 cttgatatgg ctttaagaga ttctctcgga tcatctcttt cgatcataat gcttctgata
     8401 cgttttatcc atgtgccgta caatggcact ttttcaagtc cgttttttgc tataaagccg
     8461 aactgtacag tatcgagcac agcacaaagg acaactatat cggcataacc ctgatgattt
     8521 gaaacgtata ctacgggaga gtctttgggc agattctctg tgccgtaaac atttatttcc
     8581 accccgatca tttcagaaag tttgctgccc caggtctttg tggctttgag tatgttttct
     8641 ttttcttttt tcatgtctcc ggaaaacctg ccggagtcta tttctttttt gtattcattt
     8701 agatacctca aggaccggta gatctttatg acctgtggta tgttcctaat tattttcata
     8761 aaaatattcc ccttcaaaga tcaaaaattt agtttgtctg aatgacagta taccatgttt
     8821 ttaatgactt ttagaaaaaa agagaatata atattcacag aagcaaggag ccctcatgac
     8881 gacaaaaaag aaaaaaacag gaaaatatgc cgctgcggtt ttatgtgcgg tggccataat
     8941 tctctatgaa gctgcagtcg tctatggagt gttcaaactt ggacttgacg aaaaaattcc
     9001 cgtctgggca gtcgttctca tcataacgat accggccctg gcggccgcag ggataattgc
     9061 ggcacttata cttcgtatca aagaaatcaa aggaggagaa gaagatgttg cttctaagta
     9121 ttaattacat accggatcag gaaattgagg cattgggcat tgtaaagggt aatgtcgttc
     9181 agtcaaagaa tgtaggcaga gatatcatgg ccggcatgaa aggtcttgtg ggaggagagc
     9241 tttcgggtta taccgatatg ctgaatcaag caaggcagat agccaccaaa agaatggtgg
     9301 acgaagcaga agccatgggg gcagatgcga taataagggt agaatatgca tcttctgcaa
     9361 taatgcaggg agcggcggaa ataatagcat acggaacagc tgtgaaattc aaaaaatgac
     9421 aaaaaaaaca aaaaccttac aagtggcaat attgagcgga gcagttgttt gtttcgctct
     9481 tataacatac actgtaacaa cagggatctc ttccggattt gataatgctg taatggagtt
     9541 tttttacgga ataagaaaac ccggtcttga tacactgatg gaacttataa cttatatggc
     9601 aaactgggag acgatagtca taatctgcct tttgctggtt tgttttccgc agacgagaaa
     9661 gaaggccgga ataagcgttt ctattgcagc gcttttgtcg actttgataa aagctgtcat
     9721 taagacggca gtggcaaggc caagacccga agaaagtctt tttcttattg agcagagcgg
     9781 atccgcttat cccagcggac atgcaatgac aggaatggca gtttatctgc tgctttttct
     9841 ttttacaagg gaatatataa aggacagcag aaagaaaaat ttgctgtcct ttgtttttgt
     9901 ttttgccgct tttgccgttg gcataagcag agtatacctt ggagttcatt tccctactga
     9961 tgtactgggg ggatggacat tgggttttgc tactgtcata ttatccctta ttatatggga
    10021 gattctaaaa aaacgtttgc cctactgttc ggatctgtca taaaaaagac tttttccgtt
    10081 gactcccaga gggatgccga agcacactct tactttttca gagaaaagat ccattctcat
    10141 ggcacctttg ccggcagaaa aaagaactct attatctatt ctgtgatccg ccgcaacgga
    10201 aacggcggaa cctattgcta tgcccaggtc tgtaactttg aaagcacagt tcgcgccggc
    10261 tttttccatg gccgcacaat cggggaatcc gcacaaactg cagtttgtaa gtcccaaagg
    10321 ttcatttttt actgctataa gaaccacaca atgactgtta tccacatttt tcccgtctct
    10381 ttcataaaaa tcctcaccgg tttcctttgc gatatctttc atgtgccccg ccagcagatc
    10441 tttgtccttt ccttcgatta tgaaagtttc gatctcgtct acaccgcaac ccttgggggc
    10501 tgtccttact gctgcagcca taagatcagc cactgcaaag gcagcttttc tttcggcatc
    10561 tccgcttttg attgtcattt tttatctcct cctgtgttgt tttttgataa aaaaaaatac
    10621 atttctgttt gcgtttttat tatatcattt ttttggttgt ttttaccact aaataagtat
    10681 aaaatattgg tacaaaaacc aaagggagaa aaacatgaga gatgggtaca acattaaaaa
    10741 aagcagatgg cttacggcgg gaagcatcgg agcagtggtt tgcatcctga tcatcgtttt
    10801 ttcttattac agctacgaaa aaaaactgga tgaaaacctc aaggattatg catacagcaa
    10861 gcttgatgaa accgttaagg atcaggcatt tgtcataagt tcaaagatcc atgacgaaag
    10921 caggatactc gtcaatatag cagattattt ggcaaaagaa ggaacagaca caaaaaagaa
    10981 tttgcttatg gcatccatgg aaaaaggaag tacatttgaa gctataggtt acagcgataa
    11041 aaacggaata ataacagaac cggatgggaa acaaacagat atatcaaaaa gagagtattt
    11101 tgaaaaagcc ataaaaggcg aagaaactac tcagtatgta gaaaaaagca ttgaagaggg
    11161 aaaaccggaa atcattatag ctgtaccggt aaaagacggc tcggaaataa aaggagttgt
    11221 tgccggtaaa ttcggtactg accaggtgga aaaagccttg aaaaccgaag atttcggagg
    11281 aaaaggctat ttttgtcttt gtaacagcaa aggaaccgtg gtagctgcta caaaaagaaa
    11341 aagctgtatt ttcaataatg cagagaatat ttttgagaaa tatgagaata tgcaaataac
    11401 aggaggagac acaatagaaa cggtaaagga gaaaataaag gcaaaaaaaa gcgggtacat
    11461 tgaattttct tataaaaaca gaagttacta tacaagatat gttgcaaaag ccccgagttg
    11521 gatcctcctt tatacggcac caacggaggt catagaagaa aacatgtctt tcatgaagaa
    11581 aggaacagta gagcttgtaa taggacttat gatcgccatg gcagtaattt tcggagcatt
    11641 attctatgaa cagacaagag caaccagaag atataagcag ataaatcacg aaatgaacaa
    11701 tatcaatgaa agtctcgaca ttgcattatc acagactcca agaatgatac tggattatga
    11761 caagaaaacg gatactgcaa aggtatttaa aaactacatg gatgaaaaag catatcccat
    11821 aggacatgtc tttggggagt tcagtgaaag cgacggtttt gtatatgttg aagacaaaga
    11881 aatacacaaa aggataataa aaagaacaga gcaggatgaa agggcggatg acggatacat
    11941 acaggaatat gtgcgttata catttgacgg cggaagcaca ttcatatggt gttacgtcac
    12001 aggacaaagg aaactggatg agcagggaaa cattgaaggt gcaatttttg ttctggaaga
    12061 tgtaaacgtg gataaggaag aactgaaaaa tatagaaaag aaagcggaaa cggatctgct
    12121 cacaggactt ttgaacaaaa aattcacaga aaaaaaagta acagaactga taggacctaa
    12181 aaaaaccgaa ggatttcttt tcatgatgga tttagatgat ttcaaaagag taaatgacga
    12241 gaaaggacat gccataggag atatcatcct ggaaaaaacc gctgacgcag tcaggagatc
    12301 ctttagagaa gaagacataa agggaagagt aggcggagat gaattccttg tatttatgcc
    12361 gggcaccaca tcgggaaagg gagcagcgga cagagcaaat aaaatagcag aggaactgaa
    12421 gaaaatcagt gaatacgttc cgggaacaga catcacctgc agcataggta tagccagcta
    12481 cccaaaggac ggaagaacat tcagggaact ttacgaagct gtcgataagg caatgtatca
    12541 cgtaaagaga catggcaaaa aaggatattc cttcacaaaa gg
//
LOCUS       DAONHK010000022        12575 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_3937_length_12575_cov_4.592812, whole genome shotgun sequence.
ACCESSION   DAONHK010000022 DAONHK010000000
VERSION     DAONHK010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 12575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12575
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3937_length_12575_cov_4.592812"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..718
                     /locus_tag="PLM92_01600"
     CDS             <1..718
                     /locus_tag="PLM92_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HPR23867.1"
                     /translation="SPGDKINITVPTGNFGNILSAYYAGEMGIPVNRLICASNKNRIL
                     MDFINTGVYDLHREFYLTNSPSMDIIISSNLERLLWHLSGNGEEIKELMENMEKEKRY
                     AVSPAIMKGMEKFYGGYTDVDATLRVMGDMYRENGYLIDTHTAVAYDVYDQYRKDTGD
                     ETPALIAATASAYKFADSAAEAIGIGKGNDGFESIKKLAAETGVPVPKALKNLDKKEI
                     RHEGVIDIKAMKDSVREAIL"
     gene            721..1998
                     /locus_tag="PLM92_01605"
     CDS             721..1998
                     /locus_tag="PLM92_01605"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HPR23868.1"
                     /translation="MKKIAGNDLIIFILCALVLLWPLGTAKIAYGETGLSFPAENGEP
                     FPAETGGYFPADNEADVLTRETSVTGITTVYKAKKGTILTDVITVSPALGRTVYLQRY
                     DETLGQWVTMTEYVSEDTETSQVTITYPDELYNKTWSVWRIYLPEEEIANQNDPEIIT
                     DRFSAYESQAIKITATQIQNLYLYGKGAIIMCVDTGETLYEKSAKKKLYNASTTKIMS
                     AIVAIERKSMGSKVRISKKVTRTPYRELFMKKKDRFYLRDMLYAMLITSSNDASVAVA
                     EKVGGSVSRFAKLMNKRAKSLGCVNTHFVNPHGLHSKKHYSCAYDLALMTKQAIKYST
                     FLKAVGKKSYKFKNVKKTRKYKVKNGNSLLGKYQGVIGGKTGYTGPAGYCFVSIFKYQ
                     GKTYITVTLGSKTGSKRWTDTKRMLSYIRTYCK"
     gene            2097..2184
                     /locus_tag="PLM92_01610"
     tRNA            2097..2184
                     /locus_tag="PLM92_01610"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2130..2132,aa:Ser,seq:gga)
     gene            complement(2281..2739)
                     /locus_tag="PLM92_01615"
     CDS             complement(2281..2739)
                     /locus_tag="PLM92_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013842064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2680 domain-containing protein"
                     /protein_id="HPR23869.1"
                     /translation="MKNMRKLIIAGIVGLTLVAGTVTAFAASQYNTPAEAVAGLSGRE
                     VQSVIDERAQTGKTFGAIANEAGILDKFKAEMLKMKKNMVAERVADGSMTQEQADAII
                     ARIEKNQANCDGTGTGCEGNGMGLGTGAGFGQNNDQGRGMGNGLRDGSCK"
     gene            2908..3594
                     /locus_tag="PLM92_01620"
     CDS             2908..3594
                     /locus_tag="PLM92_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006805903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPR23870.1"
                     /translation="MPMILIADDNKQIVSILEEYAKKEGFAVTTAFDGDTALDKAMTT
                     LFDIILLDVMMPGMDGFEVCREIRKQSNVPIIMITARGEDFERIMGLDIGADDYIVKP
                     FSPEEVMARIRAILRRIERDDDKKVHIFTFRDLTVNLDEYVTEIDGIRISLTKKETEL
                     LWTLGINKNKVFSRDNLLNSLWGYDYYGDTRTVDSHIKRLRAKLDVVPHDGWEISTIW
                     GVGYKFEVMS"
     gene            3591..5057
                     /locus_tag="PLM92_01625"
     CDS             3591..5057
                     /locus_tag="PLM92_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019850246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPR23871.1"
                     /translation="MKNKIRFRLFLYFSGSLLVFSLIIGLVFFALFSFHNMNVHKAQL
                     EDRATSIAGALGEFWADSPNQVQGRGMVGQGKGYGAYLRFLDDIAMTDIWIVDRNLDQ
                     ISRGHEQDSLTYNDLPSDAEAVIMAAMDGEITFSENFGAFVGAPSITVASPIALSSGE
                     IVGVVLLHEQIKNIRGATENGLIILLLSVVTAVVVSFFISSALAGRFTKPLGKMKTAA
                     LQISGGDYSVSTNVIQDDEIGELATALDSMAEKLHDASKESLKLENLRRDFVANVSHE
                     LRTPVTVIRGSLEALCDGVVTEERMVEDYHCQMLSESIYLERLVSDLLELARLQNPDF
                     TMEMSEVSLKDIAQDAVRSIQRIAETKTIEIQFSCIGDNFIVLGDYGRLRQVLIILID
                     NAIKFSPVEGIVEVVLVQAQDRITLSVRDEGPGIAAEDLPYVFERFYKQRSEANKKGT
                     GLGLAIAKQIADRHNAVLKAINRPDGGCEFILSIDITK"
     gene            5392..6492
                     /locus_tag="PLM92_01630"
     CDS             5392..6492
                     /locus_tag="PLM92_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006352926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPR23872.1"
                     /translation="MNDADLTKYMNDSVKLLIRDIIKNTFNDPKETAFLVKCRKQARK
                     SMKLRTEQEKKGHHIPPFLIASITKECNLTCVGCYARENGICRGDQPPDLMTPREWAG
                     LFDQAGACGIQFILLAGGEPLLRKEVLEQASKFPDMVFPVFTNGTLIDDEYIKFFDLY
                     RNMVPVLSIEGGEETTDIRRGRGTYRVLNEKMKLLKDSRLLYGTSITVTQTNLDEVTG
                     EDFVAKLEDNGCRIVFYVEYVPIDSSTREIALSNQERDRLAGNLLKLKREHPSIFFFA
                     FPGDEQAMGGCLAAGRGFFHISPTGNAEACPFAPYSDMDLRHNTLIEVLQSEFFSKLK
                     TENLVGAEHIGGCTLFEERDNVEKILKEITRC"
     gene            6665..7018
                     /locus_tag="PLM92_01635"
     CDS             6665..7018
                     /locus_tag="PLM92_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014117645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HPR23873.1"
                     /translation="MDLKEVMDQKKFVIVGDTLNEEKYASKIKRKMTKNGYEVSAVGK
                     ELESINDVGGDIDVIDLCINPVKGLKLMKECKKDFKTVVIQPGAESPELTSYLKENDI
                     EYLEGCLLVGLKLYK"
     gene            7027..8487
                     /locus_tag="PLM92_01640"
     CDS             7027..8487
                     /locus_tag="PLM92_01640"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPR23874.1"
                     /translation="MKNIPADDKDRLILEELETTEERFKIIVENAPIGLSVWGTETFN
                     CYEINQMTLDIMGRTKKEVIRESWARFTHPDDIEENIVLLDKMVKRETDKFSLEKRFF
                     RSDGTMVWARITNVFFGYSKGGDHLHLTMMEDVTEKKRIHDELREMSYKDQLTGLGNR
                     RYFEELFVSMDKPENLPLTLLLGDVNGLKLTNDTFGHEAGDRLLQKVASDIESITRNI
                     GKAARIGGDEFVVLLPGFGEEEGKLVAAEIIARIAGDKIDETIVCSISIGRATKTRPE
                     QSMKNIYKTAEYKMYRNKLEESRRMRGRTIREIVKNLNEKTSREKEHSERVAFLCGGI
                     ADTLGFSNKQKADLNVAALLHDIGKIGIRKEILDKETPLTEEEYEEIKKHPEKGYQLL
                     RGTNEFSHLAEAILSHHERFDGKGYPREISEQDIPYYGRIICIADAYDAMTGYRPYKK
                     SMSKEEAIEEIKRCSGTQFDPELAEIFIGYIKEVDR"
     gene            8484..10577
                     /locus_tag="PLM92_01645"
     CDS             8484..10577
                     /locus_tag="PLM92_01645"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HPR23875.1"
                     /translation="MKRLVTALTAVVLAFCLSLPSGAIAASDEQTGNEKNRISIMFTG
                     DMHSHLDEHKGQGGFARLKTKADEINQKYPESFFFDAGDFSMGTPFQTIFESDASELM
                     MMGELGYDATTLGNHEFDYGAGGLAKMLQKAAENVETITEITRVYDPETYTYSTLTTQ
                     RQSMPALVQANIDWDKTLSGKKTKEDGQKLKKAMDKYGAQDYAVIEKQGISVAVFGIM
                     GEDSVDMMPEGGVKWKDRISRAKEIVNEIERNGEADMIVCLSHGGYYESLGKDSEDVE
                     LAKEVEGIDLIISGHSHEATETPIREGDTVIVAAGMNAENLGHIVLKKAGDAYDVDSY
                     RLNSLNSNVEEDYSIKSLVSSYKNQVNYRYLADYGFTYDEVLAENEYDFSSIDGFVNN
                     YGEDTLADLMTDSYIYAVKEVEGDDYEKVDAAVIPSGIVRASLEKGDITTADAFALSA
                     IGMGADGTPGYPLVSVYLTGKELKALVEVDASASDAEDGTILHFAGINYSVNKHRIIM
                     NRATDIELYTEDGKKQEIEKGRLYRVVTDLYSCQMLGLVDKKSHGLLSVMPKDKKGKK
                     INNYEEHIIKDGDEEVKVWYAAASYIDSFKNGKVPEEYSRNQGREVTDNTFNPFALLK
                     QPNTVGLMLIGVILIPVVIIAGLIIALRKRIIRRRGYDKSIFKKKTRRRDRPKMKMRK
                     MNISRSSRWNRRKKY"
     gene            10579..11247
                     /gene="rdgB"
                     /locus_tag="PLM92_01650"
     CDS             10579..11247
                     /gene="rdgB"
                     /locus_tag="PLM92_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005999800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HPR23876.1"
                     /translation="MERIVAASRNRNKIREIGRITGKFGIEIISRDDAGVPKTEIEED
                     GTTFEENSYKKAFEIMKLCGMPTLADDSGIEVDCIGKAPGVYSARFAQPEVSETGEKA
                     EDADAEMPTNDPQEIQDKLNNKKLISMIRDFSYEERRGRFVCVITMVWPDGRKLTARG
                     EVEGHILLEERGDGGFGYDPLFVPLGHNRTFAEMSSEEKNSMSHRGKALTELARLLSA
                     QYKK"
     gene            11244..12092
                     /locus_tag="PLM92_01655"
     CDS             11244..12092
                     /locus_tag="PLM92_01655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HPR23877.1"
                     /translation="MKKRFFTMLTLGIKQFRDPYYQGFAGQLSFYFMLSLVPVIILIS
                     QILGGIFGSSLDLAVDWILQYTKGVVAEELNNLLSYEFATGNNILLLLIALWASSRAQ
                     FAMMRITNFTFTEGRSTGRGYWRERFRAVITMFLTLLAIIFSLLVLVYGEQIFGLVLG
                     GIGAEKGAAEIWLKLRWPVAALLYFLMISYNYYTLPTQKIKFREVIPGSIFASLGLLI
                     VTLIFATYVGRIAAYDILYGSLASIVALLFWFYFLAWVLCLGVLFNKVWRDTGENGKN
                     NEDNFV"
     gene            complement(12142..>12575)
                     /locus_tag="PLM92_01660"
     CDS             complement(12142..>12575)
                     /locus_tag="PLM92_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HPR23878.1"
                     /translation="KYYVSDFPNDKVINNDKIILIPHLGASTKEAEDNCAVMAADELM
                     DYIENGNINNSVNYPSCSLGRLDPDATARVCILNKNIPAMLGKITGIMADQNINIRNL
                     TNKSKDDYACTLMDIDAHVTEEALENALNVEGIIRIRVIKQ"
BASE COUNT         3984 a         2459 c         3004 g         3128 t
ORIGIN      
        1 aagccccgga gataaaatca acataaccgt gccaaccggc aatttcggaa atatcctttc
       61 cgcatattat gcaggagaaa tggggattcc cgttaaccgg ttgatctgtg catccaacaa
      121 aaaccgtatc ctcatggatt tcatcaacac gggagtatat gatcttcaca gagaatttta
      181 tctgaccaat tcaccatcca tggatattat catatcaagc aatctggaaa gactgctctg
      241 gcacctttcc ggaaacggcg aagaaataaa agaacttatg gaaaacatgg aaaaagaaaa
      301 gagatatgca gtgagtcccg ccatcatgaa aggcatggaa aagttttacg gcggatatac
      361 ggatgtggac gccactctta gggtcatggg agacatgtac agggaaaacg gatatcttat
      421 agacactcat actgccgttg catacgatgt atatgatcaa tacagaaaag acacgggaga
      481 tgaaacacct gcactgatcg cagcaacggc cagtgcctac aaattcgccg acagtgctgc
      541 ggaagccata ggtataggaa aaggaaatga cgggtttgag tccatcaaaa agctggcagc
      601 tgaaaccggg gtacctgtgc ctaaggcatt aaagaatctg gataaaaaag agatacgcca
      661 tgaaggtgta atcgatatta aagccatgaa agattcagtc agggaggcta ttttataatc
      721 gtgaaaaaaa ttgccggaaa tgatttgatc atattcatcc tttgtgcttt ggttttgctt
      781 tggcctcttg gaactgccaa aattgcatac ggcgagaccg gactatcttt tcctgccgaa
      841 aatggagaac cttttcctgc cgaaaccgga gggtattttc ctgcagataa cgaagctgat
      901 gttttaactc gggaaacatc agtaacggga ataacaacag tatataaagc aaaaaaaggg
      961 accatactta cggatgtgat aacagtatca cctgcattgg gaaggacagt atatcttcag
     1021 aggtatgatg agaccctcgg ccagtgggtt accatgacag agtatgtttc tgaagacaca
     1081 gaaacctccc aggtcacaat aacctatccg gatgaattgt ataataaaac atggtctgta
     1141 tggagaatat atctgcccga agaagaaata gcgaatcaga atgatcctga gataattact
     1201 gatagatttt ccgcatatga aagtcaggca ataaaaataa cagccaccca aatacaaaac
     1261 ctttacctat acggcaaagg cgcaataatt atgtgtgttg acacagggga gacgctttat
     1321 gaaaaatccg caaaaaagaa actctacaat gccagcacca caaaaataat gagcgctatt
     1381 gtagccatcg aaagaaaatc catgggcagt aaggtcagga tctcaaaaaa ggtcacaagg
     1441 acaccctaca gagaattgtt catgaagaaa aaagacaggt tttatctcag ggatatgctt
     1501 tatgccatgc tcattacatc ctcaaacgat gcctctgttg ctgttgcgga aaaggtggga
     1561 ggttcggtca gcagatttgc aaaactcatg aacaaaagag ccaaaagtct cggctgcgtc
     1621 aatactcatt tcgttaatcc tcacgggctt cacagcaaaa aacactattc atgtgcttat
     1681 gacctggcat tgatgacgaa acaggccata aaatacagta ctttcttgaa agccgtgggc
     1741 aagaaatcat acaaattcaa aaatgtaaaa aagacaagaa aatataaggt gaagaacggc
     1801 aattcgctcc tgggcaagta tcagggagta atagggggaa agaccggata cacagggcct
     1861 gcaggatact gtttcgtttc aatattcaag taccagggga aaacatatat aactgttact
     1921 ctgggaagca aaacaggcag caaaagatgg accgacacaa aacgtatgct gagctacatc
     1981 aggacatact gcaaataaac aggcattgaa atattccgcg atccgacagc gtatgacagg
     2041 gaatatttat cttgaaaaat gtaaagctcc atgttaaatt aataggtggc tgctttggag
     2101 ggttgaccga gtggctaagg agctcgcctg gaaagcgagt agggtgtaac agcctcgtgg
     2161 gttcgagtcc catgccctcc gccaatatta aaagagagtg gtttatccac tctcttttaa
     2221 tattggtaga acatagtaag gggactcgaa cccacggagt gagtgaagag gttcatgcta
     2281 tcacttgcaa gatccatcgc gcagaccatt acccatgcca cgaccttggt cattgttttg
     2341 accaaatccg gcacctgtcc ctaaccccat accattgcct tcacatcctg ttccggtgcc
     2401 atcacagttt gcctgatttt tctcaattct ggcgatgata gcgtcagctt gctcttgtgt
     2461 catcgaaccg tctgccacgc gctcagcaac catattcttt ttcattttca gcatttcagc
     2521 cttgaactta tcaagtatgc ctgcttcgtt tgctattgca ccaaaagttt ttccagtctg
     2581 tgcacgttca tcgataacag actgtacctc gcgtccggat agcccggcaa ccgcttcggc
     2641 gggggtatta tactgtgatg ctgcgaaagc cgtgactgtc ccggcaacca gcgtcaaacc
     2701 tacaatacct gcaataataa gttttctcat gtttttcata ctatgacctc cttttataaa
     2761 gtgctttttt gttactgtgg gttgattata aatcccagct atggcaaaag tgtgtttttt
     2821 tatttctctt tcaatgttaa aatagatttg aaaaacataa aaaaaacata ttttcccatt
     2881 atcttatatc aggagaggaa tgacgtcatg cctatgatac tgattgctga tgacaacaaa
     2941 caaattgttt ccatattgga agaatatgct aaaaaagaag gatttgccgt aacaactgct
     3001 tttgacgggg acactgcatt ggataaagcg atgacgacgc tttttgatat aatattgctt
     3061 gatgttatga tgcccggaat ggacggcttt gaagtctgcc gcgaaatccg aaaacaaagc
     3121 aatgtgccca ttatcatgat tacagcgagg ggcgaagatt ttgaacgcat catgggtctt
     3181 gatatcggtg cagacgatta catcgtcaaa cccttttcac cagaggaagt tatggcacgc
     3241 atccgggcta ttttgcgccg cattgaacga gatgacgata aaaaggtaca tatttttact
     3301 tttagagatc taactgtcaa ccttgacgaa tacgttaccg aaattgacgg catccgaatt
     3361 tctttaacaa aaaaggaaac ggagctgtta tggacgttag gtataaataa aaacaaggtt
     3421 ttttcccgcg ataatctgct gaacagcctg tggggctacg actactatgg cgatacacga
     3481 acagtggact cccatatcaa gcggctacgt gcaaagttgg atgtagttcc tcatgatgga
     3541 tgggaaatca gtaccatctg gggcgttgga tataaatttg aggtgatgtc gtgaaaaaca
     3601 aaatccgttt tcgacttttt ctttattttt cagggtctct actcgttttc tcccttatca
     3661 taggactggt atttttcgcc cttttttcat ttcataatat gaatgtccac aaagcccaat
     3721 tagaagaccg cgccaccagt attgccgggg cattgggcga gttttgggct gacagcccta
     3781 atcaagtcca agggcgcggt atggtagggc agggaaaggg gtacggtgca tatcttcgat
     3841 ttctagacga tattgccatg acagatatct ggatcgttga ccgcaacctg gatcaaattt
     3901 cccggggcca cgagcaggat agtctgacat acaatgacct tccatcagac gcagaggccg
     3961 tcattatggc agcaatggat ggggaaataa cctttagtga aaattttggc gcatttgtcg
     4021 gcgctccttc catcactgtg gcttcgccaa ttgctctttc aagcggagag atagtcgggg
     4081 tcgttcttct tcatgagcag attaaaaata tccgtggtgc gaccgaaaac ggtcttatca
     4141 ttttactgct gagcgtggtg accgcagttg ttgtttcatt ttttatctca agcgcgctag
     4201 caggacgttt caccaaacca cttggtaaaa tgaaaaccgc agctcttcaa atcagtggag
     4261 gcgattattc ggtaagtaca aatgtcatac aagacgacga aattggcgaa ttggctacag
     4321 cactggacag tatggcagaa aagctgcacg acgcttctaa agagagctta aaactcgaaa
     4381 atctacgacg ggactttgtg gcgaacgtct cacacgaatt gcgtacccca gtcactgtaa
     4441 ttcgcggttc gctggaagcg ctttgcgatg gtgtggtcac ggaagagcgc atggttgaag
     4501 actatcactg tcaaatgctc tcagaaagca tctatctgga acgcttggtt tccgatctgc
     4561 tcgagctcgc gcgactacaa aaccctgact ttactatgga aatgagcgaa gtgagtttga
     4621 aggatattgc acaggacgct gttcgtagta tacagcgcat cgcagagaca aaaactattg
     4681 aaattcagtt ttcctgtatt ggtgacaact ttattgtact tggcgattac ggaaggttgc
     4741 gtcaagtatt gataatcctt atagacaatg ccatcaagtt ttcacctgta gaggggattg
     4801 tagaagttgt gctcgtccag gctcaggata gaattaccct atctgtacga gatgaaggtc
     4861 caggcattgc tgctgaagat ttgccgtatg tttttgaaag gttttataaa cagcgttcag
     4921 aagcgaataa aaaaggcacc ggcttaggtc ttgctattgc aaaacaaata gcggataggc
     4981 acaacgccgt tcttaaagca ataaacagac cggatggagg ttgtgaattc atattatcta
     5041 tcgatattac caaatgaata attataatga aatcacagtg gatccaaaat ctgatagcag
     5101 gaagcaggag gcgactggag gattcggtga gttggagcaa ggaccaaagg tccgagcgtt
     5161 gacctccgtc aatagaatta tcaaaaaacc ggtcttttgt ggactggttt ttattttgtc
     5221 aatgatcaag tgtcagatcg cagcaaaaca acttttccgg aaattctttt ttctacctga
     5281 tactgttgga tatcaagggc aatagtgtta aaatatatca atcaaaagat tagcgaaaaa
     5341 agcaaggggt tttgatataa caaaacagaa cacagaagga gtcatcaaac aatgaatgat
     5401 gcagacctta caaaatatat gaacgactcg gtgaaacttc tgatccggga tatcatcaaa
     5461 aatactttta acgatccgaa ggagacagca tttttagtga aatgccgcaa gcaggcgcga
     5521 aagtcgatga aacttagaac ggagcaggaa aaaaaaggac accatatacc accgtttctg
     5581 atcgccagca ttacaaaaga gtgtaacctg acttgcgttg gttgttatgc acgtgaaaac
     5641 gggatctgca gaggggatca gcctcccgat cttatgaccc caagggaatg ggcgggattg
     5701 tttgatcagg caggtgcatg cggtatacag ttcatccttc ttgcgggagg tgaacctctg
     5761 ctaagaaaag aggtactgga acaggcctcg aaatttcccg acatggtatt tccggttttt
     5821 acaaacggga ccctgattga tgatgaatat ataaaattct ttgatcttta ccgcaatatg
     5881 gtgccggttt tgagtattga aggcggggaa gagactacgg atatcagaag aggcagggga
     5941 acttaccgag tattaaatga aaagatgaaa cttcttaaag acagcagact tctttatgga
     6001 acatccatca ctgtgacgca gacgaatctc gatgaagtca caggtgagga ttttgttgcc
     6061 aaattagagg ataatggctg caggatcgtg ttctatgttg aatatgtacc gatagacagc
     6121 tcaaccagag aaatagcact gtcaaatcaa gagcgggaca ggcttgccgg aaatctgctc
     6181 aaactcaaaa gggaacatcc ttctattttc ttttttgctt ttcccggaga tgaacaggct
     6241 atgggcggct gcctggctgc cggccgggga tttttccata tcagcccgac aggcaatgcg
     6301 gaagcatgtc ccttcgcacc ctattcggac atggatcttc gtcataacac tttgattgag
     6361 gtcctgcagt ctgaattttt cagtaaactg aagactgaaa atctggttgg ggctgagcac
     6421 atcggcggat gcacattgtt cgaagaacgg gataacgtgg agaagatcct gaaagagata
     6481 acccgctgct gacccacata ccctgcgcat tttccttaaa aagtcttttg atcttccgga
     6541 ttaagcccgg tgccggacaa atcttcccat atattaagaa ttttcttatt ttattgaaaa
     6601 aaacggataa aaacattata ataaaggaac gaagtttatg ccgcagaacg gagggaaaag
     6661 cattatggat ttaaaagaag ttatggatca gaaaaaattc gttattgtgg gcgatactct
     6721 caatgaagaa aagtacgcaa gtaaaataaa aagaaaaatg acaaaaaacg gttacgaagt
     6781 aagtgccgta ggaaaggaac ttgagtccat caatgatgtg ggaggtgata tcgatgttat
     6841 agatctctgc atcaatcccg tcaaaggtct gaagctcatg aaagaatgca aaaaagattt
     6901 caagaccgtt gttatacagc ccggagccga aagcccggaa ttaacaagct atctaaaaga
     6961 aaacgatata gagtatctcg aaggatgcct tctggttggt ttaaagttgt acaaataagg
     7021 agatttatga aaaacatccc tgcagatgac aaggatcgcc tgatactgga agagctggaa
     7081 actactgagg aaaggttcaa gataatagtt gaaaatgcgc caatcggact aagtgtatgg
     7141 ggcacggaga cttttaattg ctatgaaata aatcaaatga ccctcgatat aatgggaaga
     7201 acaaaaaaag aggtcataag ggagtcatgg gcaaggttca ctcaccctga cgatatcgaa
     7261 gaaaatattg tactgcttga taaaatggtc aaacgcgaaa cagacaagtt ctcgctggaa
     7321 aaacgttttt tcaggtctga cggtaccatg gtgtgggcca ggatcacaaa tgtctttttc
     7381 ggatacagca aaggcggaga tcatcttcat cttaccatga tggaggatgt gacagagaaa
     7441 aagcggatcc atgacgaact cagggaaatg agctacaaag accagctgac cggccttggc
     7501 aaccgcagat attttgaaga acttttcgtt agcatggata agcctgaaaa tcttccgctg
     7561 acactgcttc ttggggatgt aaacgggctc aaacttacta acgatacttt cggtcatgaa
     7621 gcgggagaca gacttctgca gaaggtggcc tcggacatag aaagtatcac caggaacatc
     7681 ggcaaagcag ccagaatagg cggggatgaa tttgttgtgc ttcttccggg ctttggggaa
     7741 gaagagggca agttggtggc agcggagatc attgcccgga tagccggaga taaaatagat
     7801 gaaacaatag tatgttcgat ttccattggc cgggcaacga agacaagacc ggagcagagt
     7861 atgaagaata tatataaaac cgcagaatac aaaatgtacc gtaataaact ggaagaaagc
     7921 cgcaggatga ggggcaggac cataagggaa atagtcaaaa accttaatga aaagacttcc
     7981 agagaaaagg agcacagcga aagagtggca tttctttgcg gaggaattgc ggacacgctg
     8041 ggattcagca acaaacaaaa agcggatctc aatgtggcgg ctcttctgca tgacatagga
     8101 aagataggga taagaaaaga gatccttgac aaagagacac ctttgactga agaggaatat
     8161 gaagaaatca aaaaacatcc cgaaaaggga tatcagcttc tccggggcac caatgaattt
     8221 tcccatctgg cagaagcgat actttcccat catgaaaggt tcgacggcaa aggctatccc
     8281 agggaaataa gcgagcagga cataccttac tacggcagga taatatgtat cgcagatgcc
     8341 tatgatgcca tgacaggcta caggccctac aaaaaaagta tgtccaagga agaggcaata
     8401 gaagagataa aaagatgttc cggcacgcag ttcgatccgg agcttgcaga aatatttata
     8461 gggtatatca aagaggtgga tagatgaaaa ggttggtgac tgcattaacg gcagttgttc
     8521 ttgctttctg tttgtcgttg ccgtccggtg caatcgctgc gtccgatgag caaacaggca
     8581 atgaaaaaaa cagaatatca ataatgttca caggagacat gcattcgcat cttgacgaac
     8641 ataaaggaca gggcggattt gcacgactca aaacaaaagc agatgaaata aatcaaaaat
     8701 acccggaatc atttttcttt gatgcgggtg atttttctat gggaactccc ttccagacca
     8761 tattcgaaag cgatgcttca gaacttatga tgatgggaga gctgggatat gatgcaacga
     8821 ccctgggtaa tcacgagttt gactacggag cgggaggcct tgcaaaaatg cttcaaaagg
     8881 ccgcagaaaa tgttgaaaca ataacggaga tcacaagagt atatgatccg gaaacataca
     8941 cttacagcac cctgacaaca caaaggcaaa gtatgccggc tctggttcag gcaaacatcg
     9001 actgggataa aaccctttcc ggcaagaaaa caaaagaaga tggacaaaag ctgaaaaagg
     9061 ccatggataa atacggggcc caggactatg ccgtgatcga aaaacaggga ataagcgtgg
     9121 cagtattcgg gatcatgggg gaagactccg tagatatgat gcccgaaggc ggtgtcaaat
     9181 ggaaagacag gatcagcaga gccaaagaaa tagtaaatga aatagaaaga aacggtgaag
     9241 ccgacatgat agtttgtctt tctcacggcg ggtattatga gagccttgga aaagacagcg
     9301 aagacgtaga actggcaaaa gaagtagaag gtatcgatct tatcataagc gggcacagcc
     9361 atgaagctac ggagaccccc ataagggaag gcgataccgt aattgtggcc gccggcatga
     9421 atgcagagaa ccttggacat atagttctta aaaaagcagg ggacgcatat gatgtggaca
     9481 gttacagatt gaattctctc aattcaaatg tggaagagga ttacagcatc aaatcgctgg
     9541 tcagcagtta caagaatcag gtcaactacc ggtatctggc ggattacgga tttacctatg
     9601 atgaagtcct ggcagaaaac gaatatgatt tctcatccat cgatggattt gtaaacaact
     9661 acggagaaga cactctggcg gatctgatga cagattcata tatatatgct gtcaaagaag
     9721 tcgaggggga cgactatgag aaagtcgatg cggcggttat tcccagcggc atagtaagag
     9781 catctctcga aaagggagat ataaccacgg ccgatgcctt tgctttgagt gctatcggga
     9841 tgggagcgga cggtactccc ggatatcctc ttgtcagcgt ttaccttaca gggaaggaac
     9901 tgaaggccct ggttgaagtc gatgcctctg cttccgatgc ggaggacgga actatacttc
     9961 attttgcagg gataaattat tctgtaaata agcacaggat aataatgaac agagcaacag
    10021 acatagagct ttatacagaa gacggcaaaa aacaggaaat cgaaaaagga agactttaca
    10081 gggtagttac agatctttat tcctgtcaaa tgctggggct tgttgacaaa aaatctcacg
    10141 ggcttctttc ggtcatgccc aaagacaaga aaggaaagaa gataaataat tatgaagagc
    10201 atatcatcaa agatggcgat gaagaagtga aagtatggta tgctgctgcc agttacattg
    10261 attccttcaa gaacggcaaa gtgccggagg aatacagcag gaatcaggga agggaagtta
    10321 cagacaatac cttcaatcct tttgcactgc tcaaacagcc aaatacggtg ggactcatgc
    10381 ttatcggggt aatattgata ccggtagtaa taatagcagg tctcataata gccctgagaa
    10441 aaaggataat cagaagaaga ggatatgata agagcatttt caagaagaaa accagaagaa
    10501 gagacagacc caaaatgaaa atgagaaaaa tgaatataag caggagcagc cgctggaaca
    10561 gaaggaaaaa atactgatat ggaaaggatc gttgcagcct cacgtaacag aaacaaaata
    10621 cgtgaaatag gaaggataac cgggaaattc ggaatagaaa tcatatcaag agatgatgcc
    10681 ggagtgccaa aaacagagat agaagaggac ggcaccacat ttgaagaaaa ctcttacaaa
    10741 aaagcatttg aaataatgaa actctgtgga atgcccactc tggcagacga ttcgggcatt
    10801 gaagttgact gcataggaaa agcacccgga gtatattcgg cacgttttgc acagccggaa
    10861 gtttcagaga ccggagagaa agccgaggat gccgacgctg agatgccgac gaatgaccca
    10921 caagaaatcc aggataaact aaacaacaaa aaactgataa gcatgataag ggatttttct
    10981 tatgaggaaa gaagaggccg ttttgtctgc gtaattacca tggtatggcc cgacggaaga
    11041 aaactgacag ccagaggaga agtcgaagga catatattac ttgaggaaag aggagacggt
    11101 ggctttggct atgatccgct ttttgtccct ctggggcata atagaacctt tgcggagatg
    11161 tcttcagaag aaaaaaacag tatgagtcat agggggaagg cgctgacgga actggcaagg
    11221 ctgctgtcgg cacagtacaa aaaatgaaaa aacgattttt cacaatgctt actttgggga
    11281 taaagcagtt cagagatcct tattatcagg gatttgcagg gcagctttcg ttttatttta
    11341 tgctttctct ggtgccggtc ataatcctta tttctcagat acttggaggt atattcggat
    11401 cctctctgga tctggccgta gactggatat tgcagtatac aaaaggagtg gtggcagaag
    11461 aattaaacaa tctgctttcg tatgaatttg ccacaggaaa caatatactg cttctcctga
    11521 tcgcactatg ggcatcttca agagctcaat tcgcaatgat gaggataaca aatttcacct
    11581 ttactgaggg cagatccaca ggaaggggtt actggaggga acgtttcaga gccgtgataa
    11641 ccatgttcct cactcttctg gcaataatat tttcacttct ggtgcttgta tacggagaac
    11701 agatatttgg acttgtatta ggagggatcg gggctgaaaa aggggccgct gaaatatggc
    11761 tgaaactgag atggcctgta gcggcgctgc tttatttcct tatgataagt tataattact
    11821 atacacttcc gactcaaaag ataaaattca gagaagttat acccgggagt atttttgctt
    11881 ccctgggcct tttgatagtg actcttattt ttgccactta tgtgggaagg atagcggctt
    11941 atgacatact ctacggatct cttgccagca ttgttgcttt gcttttctgg ttttattttc
    12001 tggcctgggt actctgcctg ggagtccttt tcaacaaggt atggagagac acaggggaaa
    12061 acggcaaaaa taatgaggac aattttgtct gaaacacgtc cttatcctca tataccgatc
    12121 ccaatatact gattctcctg attattgttt tattactctt atccttatta tcccttcaac
    12181 gttaagggca ttttcaagtg cctcttctgt cacatgggca tctatatcca taagagtgca
    12241 ggcataatca tctttgcttt tgtttgtaag gtttctgata tttatgttct ggtccgccat
    12301 tatgccggtt atcttgccca gcatggccgg tatgtttttg ttcagtatgc atacccttgc
    12361 ggtggcgtca ggatccagtc ttcccagaga acatgacgga taattcactg aattatttat
    12421 gttcccgttt tcaatgtagt ccatgagctc gtccgcagcc attactgcgc agttgtcttc
    12481 agcttctttt gtggaggcgc caagatgggg aataaggatt atcttatcat tattgatcac
    12541 cttgtcattg gggaaatcgc tgacatagta tttga
//
LOCUS       DAONHK010000023        12351 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_4022_length_12351_cov_4.241867, whole genome shotgun sequence.
ACCESSION   DAONHK010000023 DAONHK010000000
VERSION     DAONHK010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 12351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12351
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4022_length_12351_cov_4.241867"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            108..905
                     /gene="proB"
                     /locus_tag="PLM92_01665"
     CDS             108..905
                     /gene="proB"
                     /locus_tag="PLM92_01665"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009106590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HPR23879.1"
                     /translation="MTALSELLKKSRKIVIKIGSNVLSSDNGTVNKEVMKDIAEQVNS
                     LMKSGKQVIIVSSGAGICGAGAIDRWSRRDDMNYKQALCAIGQVELMMAYKEYFAAHG
                     INVGQMLLTRDDFTESQRNLHIRNTLFTMVDEGVVPIINENDSVSVEEIKIGDNDTLA
                     AAAASLWNADVLIMLSDIDGVFDKDPKENKDAGLIEEIYDLDSLLENIDAKGKSDFGT
                     GGIVTKIEAAKRARKYYIPTVLMNGRKRSIIIDAAEGKAKGTTFLCP"
     gene            947..1747
                     /gene="proC"
                     /locus_tag="PLM92_01670"
     CDS             947..1747
                     /gene="proC"
                     /locus_tag="PLM92_01670"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HPR23880.1"
                     /translation="MKIGFIGTGNMGGAILKGYSKKAMVSGSEILAYDADPQKLMEIA
                     EQYRVYPAKSAGLLVEGSDIVILGLKPNIFEKVMPEISEAYADDKVVVSMAAGVSISF
                     IEKYLGKTAKVVRIMPNTPALIGQGMTAVAKNPNVDDALFSTVMDIFESTGKAEEVTE
                     DLMDCVTALSGSSPAYAYMFIEALVSAAEKNGMEREKAIVFAAQAMLGASKMVLETKE
                     SPGKLRENVCSPGGTTIEAVQVLKKRDLTGVVEEAFSAALNKSKVMTK"
     gene            1744..2280
                     /locus_tag="PLM92_01675"
     CDS             1744..2280
                     /locus_tag="PLM92_01675"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006000467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HPR23881.1"
                     /translation="MIFEEKTLSSERIYEGAILNLRRDKVTVKNGTSYREIVEHNGGA
                     VAAAINDEGKIVLVKQFRKPVECVVLEAPAGKIEKGEEPAETIQRELKEETGYTADSM
                     EFMTAIKTSVGYTDEVLYIYLAKGLTPGETDFDENEAIEVVEYDFEEAYSMVIRGEIR
                     DAKTVVAILLAKNHLQNQ"
     gene            2349..3230
                     /locus_tag="PLM92_01680"
     CDS             2349..3230
                     /locus_tag="PLM92_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase"
                     /protein_id="HPR23882.1"
                     /translation="MHIEGFIKYLTKEKKMSKNSLEAYERDVMDFFAFEEEKGIKDPA
                     ETTNTEIVGYLLKLKNEGKSAATINRKLASTRSFFNYMQNAGIISGNPTSGIKTPKVE
                     RKELEYLTIDEVDRLLDSPSEDPRGLRDRAILELLYATGIRVNEVIAANVEDVNLRIG
                     FFTCPGEHGKARIIPIGRPARQALERYIYDARDMIIKDNRSEKSLFVNYYGQRMTRQG
                     LWKVLKEYAEKEKIENKLTPHILRNSFAVHMIQNGADLKSLQELLGHEDIAATQIYLS
                     VTKNRIKDVYDKTHPRA"
     gene            3291..4385
                     /locus_tag="PLM92_01685"
     CDS             3291..4385
                     /locus_tag="PLM92_01685"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HPR23883.1"
                     /translation="MDESNRMICLIGIYLCAFVVTAALTPAAIALAKKTGAMDVPKDA
                     RRIHTKPIPRIGGTAIFAGVTIAVALALFLGFLEGENVTTSKILGILVGGVLIYILGT
                     IDDFKDLPAPVKLAGQIGCAMVVFFAGNRIDFIANYFGEGTQQLAGVVGFIITVIWIV
                     AITNTINLVDGADGLAAGIVAIASLCIAYVAYIFGYYPGCICMLAVAGGALGFLPFNF
                     YPAKTFMGDGGALFLGFMIANFSILEPVKRATMVAVLIPALVLALPIFDTLWAIIRRK
                     INGRPVMQADSEHIHHRLLRAGMGQRRTVLCMYGVCGIMGTAAVLLSRELYVEATGLM
                     AVAAMYVFIVMTDVHTKSPRIKEKNDKKNE"
     gene            4571..5572
                     /locus_tag="PLM92_01690"
     CDS             4571..5572
                     /locus_tag="PLM92_01690"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HPR23884.1"
                     /translation="MKRKPILWTVIVFMAFSVFCTPTAVLAEDDTATDPQTTQDIQGP
                     GTGEQPGENGGETADPPVLTEEPDQQMIQTPVVQEIPAVGLISAEVFGPPILKIKSTA
                     KTSVIIKWNVVKDADGYVIYRAKGKKYVKIKTIKKANTISYKNKKLKKNKKYKYRIKA
                     YYYSGNKKIYSKYSKTLSAKTKSGKVVRSAGFWKTNAGKIIKKAESKLGARYRYGGTG
                     PKSFDCSGFVYWVYKNSGAKVKKVPRMGRTTYRKYKKYNIGRNIKKAKPGDIIMFSRS
                     GSVKRITHVAIYYGNGKIIHASDYSTGVIVGRIGSRKTVAIIRLPGMGSSTSTASSK"
     gene            5708..6037
                     /gene="rplS"
                     /locus_tag="PLM92_01695"
                     /pseudo
     CDS             5708..6037
                     /gene="rplS"
                     /locus_tag="PLM92_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013361788.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
     gene            6474..7340
                     /gene="ylqF"
                     /locus_tag="PLM92_01700"
     CDS             6474..7340
                     /gene="ylqF"
                     /locus_tag="PLM92_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009644094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HPR23885.1"
                     /translation="MTNVINWYPGHMKKTKELIQENLKLVNVVVEVIDARIPISSRNP
                     VLNEILKDKPRVTALNKSDLADPITNEDWLIKLRSEKKNAAAVALNCMSGKGIKELYD
                     ILNKMRSQSKSKKPFRIMIAGVPNSGKSSLINRMTGRKSTRTGNRPGVTKGKQWLNLK
                     QQKTGSGADMQVLDTPGILWPKFEDPNIGKNLAFCGSIKDEIMDPQEMALELIAVLAK
                     DHPDLLAGRYNLKVNEIVESKPINIMELIASKRGFILPGQKIDYERTGRTVLDEFRSG
                     KIGRITLEKPNE"
     gene            7333..7995
                     /locus_tag="PLM92_01705"
     CDS             7333..7995
                     /locus_tag="PLM92_01705"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HPR23886.1"
                     /translation="MNKKHTEEYSKQKLKEMRTFEENLYNQGIYFVAGVDEVGRGPLA
                     GPVVSAAVILPIDFDVLGIDDSKKLSEKKRDMFCPIIKEKALAYGIGMAGEKTIDSIN
                     ILEATKLSMKEALKNAEIMLGEKVQHILFDAVKISEIETPQTSLIHGDSKSISIAAAS
                     IVAKVTRDAMMKSYHEQYPWYSFDRNKGYGTKAHYEGIKAHGICPIHRITFLKKIIAT
                     KQ"
     gene            8054..8917
                     /locus_tag="PLM92_01710"
     CDS             8054..8917
                     /locus_tag="PLM92_01710"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009246089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HPR23887.1"
                     /translation="MKISELLKRQMTFSFEVFPPKEDKPMEPLTETLNELARFKPDYI
                     SCTYGAGGTNVGRSKETCKAIIDAGRNVMTHFTCIGSSREEVTKKIGEYKEMGVDNIM
                     ALRGDLPEGWKDTQGYFLHADRLISHIKDKFPDMCIGAACYPEKHISAVSKEADIAHL
                     RSKQDNGAEFFLTQLCYDVEEYERFREKLKKAGVTVPVIVGIMPVLFKDGLIRMTLSN
                     GCSIPSDLADVIGKYSDDPDDFKKAGKEYTIKLMYKYMNMGIDGMHIYSLNKYKDLAE
                     IINDSGIRGVF"
     gene            8947..10623
                     /locus_tag="PLM92_01715"
     CDS             8947..10623
                     /locus_tag="PLM92_01715"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005424269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HPR23888.1"
                     /translation="MAYKSDIEIAQENKTRDIKEIAEKAGIDEKYLELYGNHKAKINY
                     SLLEEMKNKDDGKLILVTAITPTPAGEGKTTTTVGLADGLSKKAKKTFVALREPSLGP
                     VFGVKGGAAGGGYAQVVPMEDINLHFTGDIHAVGAANNLLAAMIDNHIQQGNVLGIDS
                     KKITWRRCVDMNDRQLRNIVDGLGGKPHGVPREDGYDITVASEIMAILCLSKDIDDLK
                     ARISRIVVGYTFDDKPVTAGQLKAQGAITALLKDALKPNLVQTLEHTPAFVHGGPFAN
                     IAHGCNSVMATKMALKLADYVVTEAGFGADLGAEKFLDIKCRMSGIKPSAVVIVATVR
                     ALKHHGGVSKAELEKEDLKALERGMPNLLQHVENVTKVYKLPVVVAINAFPKDTKAEL
                     DMVEEKCRELGVNVALSRVWSMGGSGASELADEVLRLCSNGNGFEEAYDLDMSIREKI
                     ESIAKRIYRASGVSFTDNAKKQMQQLEDLGFGNVPVCMAKTQYSFSDDMKLLGAPKDF
                     TLTVRSMKISAGAGFIVAITGDIMTMPGLPKVPAAENIDVNKDGKISGLF"
     gene            10625..11257
                     /locus_tag="PLM92_01720"
     CDS             10625..11257
                     /locus_tag="PLM92_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HPR23889.1"
                     /translation="MTFTKKNCEEFVDMLAGSDPVPGGGGASALVAAVGTALGNMVGS
                     LTVGKKKYDDVQKDISILKEKASMLQKDFLLLVQKDAIVFEPLAKAYGMPESNQEEKT
                     EKTKVMEECLRRACSVPFEIMEKCCDAIEVCDGFAKKGSRMALSDAGAGAVFCKAALK
                     AASLNVFINTKSMNDRAYAEEINERAEKMLEEYTAFAEAVYKNVECELKG"
     gene            11261..12130
                     /locus_tag="PLM92_01725"
     CDS             11261..12130
                     /locus_tag="PLM92_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013978151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HPR23890.1"
                     /translation="MAKLLTGKEVADALNKEKAEKTKTLKAEGIIPALAVVRVGAKPE
                     DETYEKAILKKAEKTGIDVDQYTLPEEASTEEVVEIIKMLNRDKDIHGILVMRPLPFA
                     IDDEKVCGVLDAEKDVDGITEKSMAALYSGKDDFFAPCTAAACMEILEFYDMDPAGKK
                     STVLGRSNVSGRPLAMLLLKADSTVTLCHSKTPDTAEICRKSEIIIAAAGKAGMADRS
                     FFNKNQIVIDVGINVSTDGKICGDVDTEAALSEVHAITPVPGGVGSVTVSVLMRHVAE
                     AAISSCQNPYPRS"
     gene            complement(12115..>12351)
                     /locus_tag="PLM92_01730"
     CDS             complement(12115..>12351)
                     /locus_tag="PLM92_01730"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006877202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HPR23891.1"
                     /translation="ANVSGIICDGAKSSCAAKIASSIDAAIMGHEMTKSKMTFPCGEG
                     IVEDDIEETIDNIGLIGKEGMKETDIDHASYDRG"
BASE COUNT         4261 a         2231 c         3022 g         2837 t
ORIGIN      
        1 tcaagggttt tttataaaat ggaatctctt ttttgaaaat caatttttat ttgcggtaca
       61 aagatgatat aatgatttca tcttaaaaca aggagaacgg atatagaatg acagccctaa
      121 gcgaattgct aaagaaaagc agaaaaatag ttatcaagat aggaagcaat gtactttcca
      181 gtgataacgg aactgtaaac aaggaagtga tgaaagacat agctgaacag gtaaacagcc
      241 ttatgaagag cggtaaacag gtgattatag tctcttccgg ggctggcata tgcggcgccg
      301 gcgccataga cagatggagc cgcagagatg atatgaatta caaacaggct ctttgtgcca
      361 tagggcaggt agaattgatg atggcataca aagaatattt tgccgcccat ggaataaatg
      421 tagggcaaat gcttctgacc agggatgatt ttaccgaaag ccagcgaaat ctccatataa
      481 gaaatactct cttcaccatg gtcgatgaag gagtcgttcc cataataaat gaaaacgaca
      541 gcgtaagcgt cgaggagata aaaataggcg acaacgacac tttggcggcg gcggcggcca
      601 gtctttggaa tgcggatgtt cttattatgt taagcgatat tgacggagtc tttgataaag
      661 atcccaaaga aaacaaggat gcgggattga tcgaagaaat atatgatctg gattcactcc
      721 ttgagaatat cgatgccaag ggcaagagtg atttcggtac cggaggcatt gttacaaaaa
      781 tagaagcagc aaaaagagca agaaagtact acatacccac agttctgatg aacggaagaa
      841 aaagatccat tattattgat gctgcagaag gcaaagctaa agggactact tttttatgcc
      901 cataaaaaca aaagggttca gatcataaga ggagaataat gaataaatga aaatcggatt
      961 cataggtacc ggaaatatgg gaggagcgat ccttaaggga tactcaaaaa aagctatggt
     1021 atcaggaagt gagatactgg cttatgatgc ggatccccaa aagctcatgg aaatagcaga
     1081 acaataccgg gtatacccgg caaaaagcgc aggccttctt gtggagggca gcgatattgt
     1141 aatactggga ttgaaaccca atatttttga aaaagtcatg ccggagatat cggaggcata
     1201 tgcagacgat aaggtggtcg tgtctatggc ggccggagtg agtatatctt ttattgaaaa
     1261 gtatctgggg aaaacagcaa aagtggtcag gataatgccg aatacacctg ctcttatagg
     1321 acaggggatg acggcagttg caaaaaatcc caatgtggac gatgcacttt tcagcacagt
     1381 tatggatata ttcgaatcca ccggaaaagc tgaagaagtc actgaagatc ttatggattg
     1441 tgttaccgca ttaagcggca gcagtcccgc atatgcatat atgttcattg aagctcttgt
     1501 atccgccgcg gaaaagaacg gaatggaaag agaaaaagca atcgtttttg ccgcacaggc
     1561 catgctgggg gcttcaaaaa tggtccttga aacaaaagag tctcctggaa aactaaggga
     1621 aaatgtctgc tctcccgggg gaacaaccat agaagctgtt caggtactta agaaaagaga
     1681 tctgacaggt gtagtagaag aagccttcag tgccgccctg aacaaatcaa aggtgatgac
     1741 aaaatgatat ttgaagaaaa aaccctgagc agtgaaagaa tatatgaagg tgccatactg
     1801 aatctcagaa gagataaggt gacagtgaaa aacggcactt cctacagaga aatagtcgag
     1861 cacaatggtg gggctgttgc agcggcaata aacgatgaag gaaagatagt tctggtgaaa
     1921 cagttcagaa aacccgtcga atgcgtagtt ctcgaggctc ccgccggcaa aatagaaaag
     1981 ggagaagaac ccgctgaaac aatacaaagg gaactaaaag aagaaaccgg atacaccgcc
     2041 gattccatgg aattcatgac agcaattaaa acttccgttg gatatacaga tgaagtttta
     2101 tatatttatc tggcaaaggg gcttacaccg ggtgaaacgg atttcgatga aaacgaagcc
     2161 attgaggtgg tggagtacga ttttgaagag gcatacagca tggtaatccg gggggagatc
     2221 cgggacgcaa aaacagtagt tgccatatta ctggcaaaaa accaccttca aaatcagtga
     2281 ttgttattgt caaccaaatg catgtacggt atactgatgt caggttttct ttaagcgggg
     2341 gatgatcgat gcacattgaa gggttcataa aatatttgac aaaagagaaa aaaatgtcca
     2401 aaaactcact ggaggcatat gaacgtgatg taatggattt ttttgctttt gaagaagaaa
     2461 aaggaataaa agatcctgca gagaccacaa acactgaaat tgtcggatat ctgcttaaac
     2521 tgaaaaacga aggtaaatct gctgctacca tcaacagaaa actggcatcc accagatctt
     2581 tttttaacta tatgcaaaat gccggcatta tttccggcaa tcccacatcc gggatcaaaa
     2641 caccaaaagt agaacgtaag gaactggaat atcttacaat agatgaagtg gacagattac
     2701 ttgattctcc ctcggaggat cccaggggac tgagggacag agcaatactg gaactgcttt
     2761 atgccacagg tataagagta aatgaagtca tagcggcaaa tgtcgaagat gtgaatctaa
     2821 ggataggttt tttcacatgt ccgggagaac atggcaaggc caggataata cccattggaa
     2881 gacccgcaag acaggctctg gaaagatata tatatgatgc cagggatatg ataataaaag
     2941 ataacagatc ggagaaatcc ctttttgtga attattacgg ccagagaatg acaagacagg
     3001 gattgtggaa agtgctgaag gaatatgccg aaaaagaaaa aatagagaac aaactcaccc
     3061 cgcacatatt gagaaattcc tttgcagtac acatgataca gaacggagca gatttaaaat
     3121 ccctgcagga acttctcggg catgaggata tagccgccac ccagatatat ctcagtgtaa
     3181 ctaaaaacag gataaaagat gtttatgaca agacccaccc cagagcatga gatcgcatat
     3241 tttttgatga taggagaagc atttgacggg gcaggaggaa aatacagata atggatgaat
     3301 cgaacaggat gatttgcctg atagggatat atctttgtgc atttgttgta actgccgccc
     3361 tcacgccggc agctattgct ttggctaaaa agacaggagc aatggatgtc ccgaaggatg
     3421 ccagaaggat acataccaag cccataccaa gaataggcgg tactgcaata tttgcgggag
     3481 ttacaatagc agtggccctg gctttgtttt tagggttcct ggaaggagaa aacgtcacca
     3541 catcaaaaat tttaggcata ttggttgggg gtgttctgat ttatattctg gggactatcg
     3601 atgattttaa agatttgcct gctcctgtca aactggccgg acagattgga tgcgcgatgg
     3661 tggttttctt tgcgggaaac cgtatagatt tcattgccaa ctattttgga gaaggcaccc
     3721 agcagctggc aggagtagtg ggttttataa tcacagtcat atggatagtt gccatcacca
     3781 atacgataaa ccttgtggac ggtgctgacg ggctggcagc gggcatagtt gccattgcat
     3841 ctctttgtat tgcatatgtt gcatatatct tcgggtatta tcccggttgt atttgtatgc
     3901 tggctgtggc aggcggggcc ttgggctttc ttccttttaa tttctatccc gccaagacat
     3961 tcatggggga cggcggggca ctttttctcg gtttcatgat agccaatttc tccatactgg
     4021 agccggtgaa gagggcgacc atggtggcgg tccttattcc cgccctggta cttgctctgc
     4081 ccatatttga tactctttgg gcaataataa gaagaaaaat caacggcaga ccggtaatgc
     4141 aggctgacag tgaacatata catcacaggc ttttgagagc cggcatggga caaaggcgaa
     4201 cagttctttg catgtacgga gtatgcggta ttatgggaac agcagcagtg cttttaagca
     4261 gggaacttta tgttgaggca acaggactta tggcagtggc agcaatgtat gtgttcattg
     4321 taatgacaga tgtacataca aaatccccac gcataaaaga aaaaaatgac aaaaaaaatg
     4381 aatgatcgca aacacaacat attgcgcccc aagggcgctt ttttaatgat ttgaaggcaa
     4441 tatgctgtat taagtatgtg agtgttttgt gaacatcaac tttttatatt gacactaatg
     4501 tgacaaaatg gtaacattca ttatgttggg atccaaagac aaattagaaa tgtgaaaccg
     4561 gagagtattt atgaaaagaa aaccaatatt atggacggta atagttttta tggctttttc
     4621 agttttttgt acgccgaccg cagtactggc agaagatgac acagcgaccg atcctcagac
     4681 aacacaggat attcagggac ccggaacggg agaacaaccc ggagaaaatg gtggagaaac
     4741 agcagatcca ccagttctga cagaggaacc ggatcagcaa atgatacaga cacccgtcgt
     4801 tcaggaaatt cctgccgtgg gactcatatc tgcggaggtt tttggtcctc ccatacttaa
     4861 gatcaagtcc accgctaaaa catctgttat aatcaaatgg aatgtcgtaa aggatgcgga
     4921 cggatatgtg atctacaggg ccaagggtaa gaaatatgta aagatcaaga caataaaaaa
     4981 agcaaatacc atcagctata aaaacaaaaa gctcaagaaa aataaaaaat acaagtacag
     5041 gataaaagca tattattatt caggtaataa aaaaatatac agcaaatact caaaaacctt
     5101 atcagcaaaa acgaaatccg gtaaagtagt aagaagtgcc ggattctgga aaacaaatgc
     5161 cggaaaaata atcaaaaaag cagaatcaaa actgggagcc agatacagat acggaggtac
     5221 cgggcccaaa tcatttgact gtagcggatt tgtatactgg gtatacaaaa acagcggggc
     5281 aaaagtaaaa aaagtcccca gaatgggacg tacgacatac agaaaatata agaagtacaa
     5341 tataggaagg aacatcaaaa aagccaaacc gggagatata ataatgtttt ccaggagtgg
     5401 tagcgtgaaa agaataacac atgttgcgat ctattacgga aatggcaaaa tcatacatgc
     5461 ctctgattac agcacaggtg tcatagtagg aagaataggc agcaggaaaa cagtggctat
     5521 aataaggttg ccgggcatgg gatcaagtac aagcacagca tcttcaaaat agtttagata
     5581 agaattgaga tataaaaaac cccttgcatt tgcaggggtt ttgtgctata ttgttgtagt
     5641 tattacgggc ggtcctctga gtatgagcag ataccaagaa cgtcttggag ggaaggagga
     5701 atcacaaatg gatgtaatac aggcaatcac agatgaatat aagaagagtg atatccctgc
     5761 gtttgatgtg ggcgacactg taaaagtgca tatcaaaata aaagaaggct caagagaaag
     5821 gatccagata tttgaaggat ttgttctgaa aagacaacat ggcggagtca atgagacttt
     5881 cacagtaaga aggatagctt caggtgttgg tgttgaaaaa acattcccgc ttcattcacc
     5941 gagaatagac aaaatagaag ttgtcagaaa aggtaacgta agaagagcta agctgaatta
     6001 tatgcggaag agaacaggca aatcagcaaa gatcaaatca aagatataca cagaagaaca
     6061 actgaaagcc atggaagctg ccaggataag agctgaggaa gaagctgcga aggcagtgca
     6121 ggaggccaaa gccgcagcag aagctgaggc tgccgaaaca gccgaagcag aaggcgttaa
     6181 agaggttgaa gcaactgaaa cagccgaagc agaaggcgtt gaagaggttg aagcaactga
     6241 aaaagtcgaa gcagaaggcg aaaccgagac agaagaaatt cccgaagcag aggcttcaga
     6301 aaccaaagca gttgaagacg aaaacaagac agacgaagaa acgaaagagt aaagcttttt
     6361 ccggaccatc ccaaaaggga tggtcttttt ttataaatta tggagtggca agcgataaac
     6421 atgcccgcag aagacaactt tctgttataa tagcgccatg gagaggcaaa aaaatgacaa
     6481 acgttataaa ctggtatccg ggtcacatga aaaaaacaaa agaacttatc caggagaacc
     6541 ttaaactggt taatgttgtt gtggaagtca tagatgcaag aataccaata tcaagccgca
     6601 atccggtatt aaatgagatc ctgaaagaca agcccagagt gaccgctctt aacaaaagcg
     6661 acctggcaga cccaataacc aacgaagact ggctgattaa gctgagaagt gagaaaaaaa
     6721 atgccgcggc agttgcatta aactgtatga gcggtaaagg tataaaggaa ctttatgata
     6781 tactgaacaa aatgcgaagt caaagcaaat caaaaaaacc ctttcgaata atgatagcag
     6841 gtgttccgaa ttcgggcaaa tcgtctttga taaaccgtat gacaggaaga aaaagcacca
     6901 ggacggggaa cagacccgga gtcacaaagg gcaaacagtg gctgaatctt aaacagcaga
     6961 aaaccggcag cggagcagat atgcaggtac ttgacacccc cgggatactc tggcccaaat
     7021 ttgaagatcc aaacattgga aagaatcttg ctttctgtgg ctctataaaa gatgaaataa
     7081 tggatccgca ggagatggca ctggaactga tagccgtact ggctaaagat catcccgatc
     7141 ttctggccgg ccgctataat cttaaggtga atgaaatagt agaaagcaaa cctatcaata
     7201 ttatggagtt gattgcatca aaaaggggat tcatccttcc cggtcagaaa atcgattatg
     7261 aaaggacggg gcggactgtt ctggatgaat tcagatccgg taagatcggc agaatcactc
     7321 tggaaaaacc caatgaataa aaagcatacc gaggaatatt caaaacaaaa attaaaggaa
     7381 atgagaacct ttgaggaaaa cctgtacaat caaggcatat attttgttgc gggtgtagat
     7441 gaagtgggca gaggccctct ggcaggaccg gtggtttctg cggcggttat actgcctatt
     7501 gactttgatg tacttggaat agatgattca aaaaaactca gcgaaaagaa aagagatatg
     7561 ttctgtccga taataaaaga aaaagccctg gcatacggga taggtatggc aggtgaaaaa
     7621 acaattgata gtataaacat acttgaagca acaaagcttt caatgaaaga agcattgaaa
     7681 aatgcagaga ttatgctggg agaaaaggtg cagcatattc ttttcgatgc cgttaaaata
     7741 tcagaaattg aaactccgca gacatcattg atccatggcg attcaaaaag tatttccata
     7801 gcggcagcct cgatagttgc caaggtgaca agagatgcaa tgatgaaatc ataccatgaa
     7861 cagtatccat ggtattcatt tgacagaaac aaaggatatg gaactaaagc ccattatgaa
     7921 gggataaagg ctcatggcat atgccctata catcgtatca cattccttaa aaaaatcata
     7981 gccacaaagc agtaaaaaaa cacttttata tatccgtaaa atattatagt atattcggta
     8041 taaggagcag aaaatgaaga taagtgaatt attaaaaagg caaatgactt tttcgttcga
     8101 agttttcccc ccaaaagaag acaaaccaat ggaaccgctt accgaaactc tgaatgaact
     8161 tgccagattc aaacctgatt acataagttg tacttacgga gccggaggca caaatgtggg
     8221 ccgcagtaaa gaaacatgca aagcaataat tgatgccggg cgcaatgtca tgacacattt
     8281 tacttgtatt ggcagttcca gagaagaagt aacaaaaaaa atcggcgagt ataaggaaat
     8341 gggagtagac aatataatgg ctctccgggg agacctgccg gagggatgga aagatacgca
     8401 aggatacttt cttcatgcag acaggcttat atcacatata aaggataagt ttccggatat
     8461 gtgtatagga gctgcatgct atccggaaaa gcatatatca gctgtttcca aagaggcgga
     8521 tatcgcccac ctgagatcca agcaggacaa tggcgctgaa ttttttctga ctcagctttg
     8581 ctatgatgtg gaagagtatg aaaggttcag ggaaaaactg aagaaagcag gagttactgt
     8641 gccggtcatt gtgggaataa tgccagtgct tttcaaagac ggacttataa gaatgacatt
     8701 gagcaacgga tgctccatac catcggatct tgctgatgta ataggaaaat acagcgatga
     8761 tcctgatgat ttcaaaaaag cgggaaagga atatactatc aagttgatgt acaagtacat
     8821 gaatatgggt atagacggaa tgcatatcta ttctttgaat aaatataaag atctggccga
     8881 gatcataaat gattcgggca taagaggcgt tttttaaaaa aaaggactta tttacaggag
     8941 ggaaaaatgg catataaatc agacatcgag atcgcacagg aaaataaaac aagagacata
     9001 aaagaaatag cagaaaaagc aggtatagat gaaaaatatc tggagcttta cgggaatcat
     9061 aaagcaaaaa taaattacag ccttcttgaa gagatgaaaa ataaggatga tggtaaactc
     9121 atacttgtaa cagcaattac accaacgccg gcgggagagg gaaaaaccac aactaccgta
     9181 ggtcttgccg acgggttatc aaagaaagcc aagaaaacat ttgtggcatt gcgtgaaccc
     9241 tcactggggc cggtctttgg agtcaaaggc ggagctgccg gaggcggata tgctcaggtg
     9301 gttcccatgg aagatatcaa tcttcatttt accggtgaca tacatgctgt cggagctgcc
     9361 aacaatcttc tggcagccat gatagacaat catatacaac aggggaacgt tctgggtatc
     9421 gatagcaaaa aaataacctg gagaaggtgt gttgacatga atgatcgcca gctaagaaat
     9481 atcgttgacg gtcttggagg caaacctcac ggtgtaccca gagaagacgg atatgacatt
     9541 acagtggcca gcgaaataat ggctatatta tgcctttcaa aggatataga tgatctgaaa
     9601 gccaggatat ccaggatagt tgtcggctat acttttgacg acaagcctgt tacagcgggt
     9661 cagttaaaag cacagggagc aataactgcg cttttgaaag atgccttaaa acccaatctg
     9721 gtgcagaccc ttgagcacac accggcattc gttcatggcg gaccttttgc aaatatagcc
     9781 cacggctgca attcggtaat ggcaacaaaa atggcgctta agctggcgga ctatgttgtt
     9841 acggaagccg gtttcggggc agatttggga gcggaaaagt ttcttgacat aaaatgccgt
     9901 atgtccggga tcaaaccttc agctgtagtt atagttgcaa cggtccgtgc gcttaagcat
     9961 cacggaggag tttccaaagc agaacttgag aaggaagatc tgaaagccct tgaaagaggt
    10021 atgccgaatc ttctgcagca tgttgagaat gtaactaaag tctataaact gccggtggtg
    10081 gtggcaatca atgcattccc aaaggacacc aaagccgagt tggatatggt tgaagaaaaa
    10141 tgcagggaac tgggagtcaa tgtggctttg agccgggtat ggtcaatggg cggatccggt
    10201 gcttctgagc tggccgacga agttttaaga ctttgcagca acggaaacgg gtttgaagaa
    10261 gcatacgatc tggatatgtc cataagggaa aaaatcgaaa gtattgccaa gaggatatac
    10321 agagcttccg gtgttagctt caccgataat gcaaagaaac aaatgcaaca gctggaggat
    10381 ctgggatttg ggaatgtgcc cgtatgtatg gcaaagaccc aatacagctt ttcggacgac
    10441 atgaagcttc tgggcgctcc gaaggatttc acacttactg tacgcagcat gaagatatct
    10501 gccggagcgg gctttatagt tgccatcaca ggagacataa tgactatgcc gggacttccc
    10561 aaagtgccgg cggccgaaaa catagacgtt aacaaagatg gaaagatatc gggtttgttt
    10621 tagcatgaca tttactaaaa agaactgcga agaatttgtt gatatgctgg caggaagtga
    10681 tcctgtgccg gggggaggag gcgcatctgc cctggtggct gcagtaggaa ccgcccttgg
    10741 taatatggta gggtctttga ctgtgggcaa aaaaaagtat gatgatgttc aaaaagacat
    10801 atccatatta aaagaaaaag cttcaatgct tcaaaaagac tttcttttac tggtacaaaa
    10861 ggacgccata gtatttgagc cactggcgaa ggcctatggc atgccggaaa gtaaccagga
    10921 agaaaaaaca gaaaaaacaa aagtgatgga agaatgtctc agacgcgcat gttcagtccc
    10981 ttttgaaata atggaaaaat gctgtgatgc aatcgaagtc tgtgacggct ttgcaaaaaa
    11041 aggcagcagg atggcattga gtgatgcagg agcaggagct gttttttgca aagcagcatt
    11101 aaaggctgca agtcttaatg tttttattaa tactaaatca atgaatgaca gagcatatgc
    11161 ggaagaaata aacgaaagag cagaaaaaat gctggaagaa tacacggcct tcgcagaggc
    11221 tgtgtataaa aatgtcgagt gtgaactgaa aggataaaaa atggcaaagc ttcttacagg
    11281 caaggaagtt gcagatgcgc tgaacaaaga aaaagcagaa aaaacaaaaa ccctcaaagc
    11341 cgaaggcata attcctgcac tggcagtagt aagagtcgga gcgaaaccgg aagatgaaac
    11401 atatgaaaaa gccattttaa aaaaagccga gaaaacaggt atagatgttg atcagtatac
    11461 actgcccgaa gaggcatcca cagaagaggt cgttgaaata ataaaaatgc taaacaggga
    11521 taaagacata cacggaatat tggtaatgag acctctgcca tttgccatag atgatgaaaa
    11581 ggtctgtggg gtgctggatg ccgaaaagga cgttgacgga ataacagaaa agtccatggc
    11641 agccctttat agcggcaaag atgatttttt tgctccatgt acggcggcgg cctgtatgga
    11701 gatactggag ttttacgata tggatccggc gggaaagaaa agcactgtgt taggccgaag
    11761 taatgtgtca ggaagacctc tggccatgct gcttcttaaa gctgattcca cagtgactct
    11821 atgccattca aaaaccccgg atacagcaga aatttgcaga aagtctgaaa taataatagc
    11881 tgcggcagga aaagccggca tggcagacag atcatttttc aataaaaatc agatagtgat
    11941 agatgtgggt ataaatgttt ccactgacgg aaagatctgt ggagacgttg atacagaagc
    12001 agctttatcg gaagttcatg cgataacccc tgtccccggg ggagtgggca gcgttacagt
    12061 aagtgttttg atgcgtcatg tggcggaagc tgccatcagc agttgccaaa atccctatcc
    12121 ccgatcataa gatgcatgat caatgtccgt ttccttcata ccctctttac caatcaaacc
    12181 tatattatcg atggtctctt caatatcatc ttctactatg ccttcaccgc atggaaaggt
    12241 catttttgat tttgtcattt catgacccat gatggcagca tctattgatg aggctatctt
    12301 tgcggcacag gatgatttgg ctccgtcaca gattataccg cttacattgg c
//
LOCUS       DAONHK010000024        12032 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_4159_length_12032_cov_4.811973, whole genome shotgun sequence.
ACCESSION   DAONHK010000024 DAONHK010000000
VERSION     DAONHK010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 12032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12032
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4159_length_12032_cov_4.811973"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..974)
                     /locus_tag="PLM92_01735"
     CDS             complement(<1..974)
                     /locus_tag="PLM92_01735"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HPR23892.1"
                     /translation="MNAEKKIAKFKRFGSVLGLERIEKLMELLGDPQEELKIIHVAGT
                     NGKGSVCRFIYEVLRANGYRAGLYNSPFVEDFSERIEYDGERIPEKELEVCTDLVIAK
                     SRVMVENGYDSPTEFEILTAVAFLYFARMKPDIVVMEVGLGGRGDSTNIIKNPLISVI
                     TSISHDHGDRLGDTIREIASEKAGIIKSGVPVVINVKGEAKAVLQEVARQKGSSIFDA
                     ERTKHRILECGPGGSVFDVFINGTEYKEIRLKMAGLHQVDNALTALAAIEILHKNSII
                     KVNPEKTRKGMAGAFMKGRFEVVDIGIPCVLDGAHNVDGIAKLTDSIG"
     gene            complement(971..3679)
                     /locus_tag="PLM92_01740"
     CDS             complement(971..3679)
                     /locus_tag="PLM92_01740"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HPR23893.1"
                     /translation="MIEKEINIAKTYDPKDFEERIYERWEKSGAFKAERDPDKTPYTI
                     VMPPPNITGQLHMGHALDQTLQDILTRWKRMQGFSALWLPGSDHASIATEVKVVEKIR
                     EEEGKEKEEIGREEFLKRAWEWKKVYGGKITRQCRKLGDSCDWSRERFTMDEGCNKAV
                     NEFFVRLYNKGLIYRGNRLINWCPECGTSISDAEVEHENKKGAYWYFRYPSKDGGKDM
                     VVATSRPETMFGDVAIAVHPSDERYKDMVGKKVLLPIVNREIPVIADTYPDPEKGTGA
                     VKITPAHDFSDFEVGERHSLERLSCIDENAKMNSLAGKYKGMDRYECRKEWVADLEKG
                     GYLVKTEEIEIPIGGCYRCGTAVEPMLSDQWFVAMEELAKPAMRAAKDGELIHVPERF
                     EKTYLHWLENIRDWCVSRQLWWGHRIPAWYCEDCGEINVAADKPESCEKCGSKRMHQD
                     EDVLDTWFSSALWPLSTLGWPDDTKDLRYFYPTSTMVTGYDIIFFWVVRMVFSAMEIT
                     GEVPFKHVFIHGLVRDHDGRKMSKSLGNGIDPLEVIHEFGADALRFMLATGITPGNDM
                     RFQEERLQMARNFANKLWNASRFVIMNIKDEEGKFLPVATIDAESERHLQPEDKWIIS
                     ATNDTIKKVTETMEKFDLGQAGQKVYEAIWNQYCDWYIEIAKGRLYGSDEGDKQVVRG
                     VLIHVLKDMLKILHPFMPFITEEIWGALPGNGNTNEDPGKFLMKSHWPSYENKELYPE
                     ETKKLEMAMEIIRAVRNIRAEVEAPPSKKLSLVILSYGHLQQTARAGERYIKKLANIT
                     NISFIEDKEDIPEDSMSSVTGGAEIFIPSDELIDYDAETLRLVKEKDRLKNEVQRIEK
                     KLANPGFVSKAPEEVISKEKEKLVKYRSMFDKVCERLAYTESKKKK"
     misc_binding    complement(3730..3917)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(4042..5661)
                     /locus_tag="PLM92_01745"
     CDS             complement(4042..5661)
                     /locus_tag="PLM92_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPR23894.1"
                     /translation="MIRRKSKIHKKHMLITVFFTMVIFLITMSVPVIAQITGAKPSPK
                     EEVIYGDLSVSGNVKDIYAVNIFETERAGKITDYGDYSSVTNLTSAKKIKNNGGMITA
                     DVGEGRFYYKGQIKKNDLPWNIDVKYFLNGETVSPGALSGATGNVRIQITTEKNQNVD
                     PSYFENYMLQISIKLDAEKCKKIKASGGTFANSGNDKIVSFSIMPGKEDIVSLTTDSS
                     AFSMEGIDITALPFSVGIEIPDMSSLEKGMTSIVYGIGQLEQGVREFEKGLKKIDAGS
                     SQLIGSSEKIKSALQIINNSLAAGQGGFDAEALGLLADALDNANDTLGEAINDIPADD
                     LGQEDMDDLIGALTYARDNGQATEEDITNLNTLIDTYDKAQIVKSIYGSADVSEVFAS
                     SSLALGAAGEGIDELQQNISLLNENYSLFHSGLLSYTEGISESTKGSSALKEGATGLY
                     QKTSGIPSLIAEKIESVIAEYDKSDYRATSFVSKKNTKVSMVQFVIRVDGIEKKDNCS
                     KCGTADKRSPGKGAGIKNGIEGITERFKALF"
     gene            complement(5665..7725)
                     /locus_tag="PLM92_01750"
     CDS             complement(5665..7725)
                     /locus_tag="PLM92_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010199326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HPR23895.1"
                     /translation="MEHIGRIIVKKRKFVVVVFIIFTAICAVSFFGVYVNYDLTDYLP
                     EESKSTKALKIMENEFDQAVPNARVMLKNVTVGEALEYKNEIENIDGISDVIWLDDVS
                     DIKKPLEIADKETSDKYYKNGNALISFAVEEGRELEITSKVYEVIGERNALEGNALAL
                     ASVQTATVWETLKATIILLPIILLLLILTTTSWIEPILFLLAIGVSVIINMGTNLLIG
                     EVSYITQSVTPILQLAVSLDYAIFLLHRFSEMQKQKDDPEEAMIAAMKKSFPAIAASS
                     ATTLFGFVALTFMDFEIGADLGFNLAKGIIFSFVSVLVFLPALTLCCLKMIKRTKHKR
                     ILPEMSGSSRAFMKISTASLILMAIIIYPCWLAQNSNEFLYESTGVVEGQRTGEDEAS
                     VKEVFGDSTAIVILLPKGEVGKEVQLCSELEKKENVTEVVSYVSSVGAQIPDEYIGEE
                     IASGFYSKHHSRIVVYTSTSAEGEEAFTTVDEVRRTAEKYYGTNVYLCGESANLRDMK
                     DVVTLDNRRVNIIAVLAIALVIMITFRSLLIPVLLVLSIKAAIWVNLAIPYFEGSPMN
                     YIGYLIVNTVQLGATVDYAILLTDTYKKKRKSMYPKEAMKESLAERFRSILLSAVILS
                     SAGFVLGALSSNQMVAEMGILLGRGAILSLIIVVCFLPALLMMLDKPLGATTYKSDFL
                     KKEK"
     gene            complement(7932..8516)
                     /locus_tag="PLM92_01755"
     CDS             complement(7932..8516)
                     /locus_tag="PLM92_01755"
                     /inference="COORDINATES: protein motif:HMM:NF014072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF111 family protein"
                     /protein_id="HPR23896.1"
                     /translation="MDLYFDCSNGISTDMVLLALLDLGADRDFVLRETDKLNICERPN
                     EIVKKKKKNLCDHNTKRSFREIEDILRKSGLDDNTKRRALKIYTVIAEAEAEVHGKSM
                     DTVHFHELGRDRAIINVVGVSAALSNLYPDNIYCSEIHDGFGHIDCDHGRIPVPVPAV
                     RAMMKGTGYRFVQDEIETEMVTPGGLGILKGSGA"
     gene            complement(8583..10769)
                     /locus_tag="PLM92_01760"
     CDS             complement(8583..10769)
                     /locus_tag="PLM92_01760"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HPR23897.1"
                     /translation="MKFKKLVCIAIALSMVVWTFAACGEKEEAKEDTFQTKITSDYSF
                     AGDVQDYLDNVDSKYAYGIAKTLAYDEEYFDNELGWRSAGSDAEHACSDYLMEEMENI
                     GLEDVEKVGVPCDKFQFNDSKLTVEGTKINLMPASYQQTGTGEDGITAEIVDCKTGFE
                     ADYEGKNVAGKIVLVQVDQANESWIDGYIRQAYQKGAAAVVSYANSGYGELNKDTINV
                     QDVCCDDLLPTAAISANEAKKIKKAIKDGNKKATLMIDAVVEKGTGTTYNVVGKIKGK
                     DSDQQTIVSAHYDKYWFGFQDDSIAIGTVLSMAKALKDSGYEPENDLVFVCHGSEEWG
                     LSNAQDDWTTGAWGMVTRGKPEWPAKTKSLFNFELNAKDDGTDKIFAVTVPELSTFVK
                     NFVDDSGLVVRSGHIKRIKAKTVDSDTMEDGVSYRWAGVPYILNGFQSGDFSATTYHT
                     QFDTKDTWDKDVYVSNANFYGAMLVYTDKMPAMELDLRATCDDLEEALNEDTAKEADA
                     DIEGYKAELADLREACEVLYDKMQQVNVNYEKLVAEEDEEGIKQCREEGAELNKQVRE
                     VYKEIQKELIGTDTWGLAIRHALVDNNIGILQGVMDGLDKQKTWTKKENGALDMAWQL
                     NGGMDYNYYLFSKEVAGQTLIQYDDTKWTGKRQFFTDKLPPVYWVGDTTYDLVRKDAA
                     GEKIDYKAAKEVYKAALDEALVDLKAEISKEVSSMRSLADKIDDID"
     gene            complement(10815..11888)
                     /locus_tag="PLM92_01765"
     CDS             complement(10815..11888)
                     /locus_tag="PLM92_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPR23898.1"
                     /translation="MRTKSILWLGVLIIAMIFSACSSTGGNELKIDPAPEVNAKLQSY
                     DGDFFSMKLPKGWEIHTVGEYENFGFRAYDPDKPSRQIFFYGNMKYFMKSSEGKEAWN
                     RYMAEGGYADAGVYADAPVLSPATTEEFYNTFDEFTSYAKKYGIDHDFPVFNDLEILE
                     TTPRNSPIASNCIEDSILRVLFKNDDIPCEGLFAAGVADTMTSYMYDVDAGYYTVYVI
                     TGISAPADEFYQLKDTLLKSLSSFRYTDRYVEQGVARSRWGTEMALQVGRTLSEAADS
                     YNEAWSNRQRVNDALSQKRSDATMGYDRLYDTETGEVYRAELGFYDQYDTHREEFENI
                     DLQPVPDDDYDLYEKEIKGYIYK"
BASE COUNT         2909 a         2974 c         2117 g         4032 t
ORIGIN      
        1 ctacctatgc tgtctgtcag ttttgctatc ccgtcaacat tatgtgctcc gtcaagcaca
       61 catggtatcc caatatctac gacttcaaat ctccctttca taaaggcccc ggccatacct
      121 ttccgggttt tttcggggtt gacttttatt atactatttt tgtgtaaaat ttcaatagct
      181 gcaagagctg tcagggcatt atcgacctgg tggagtcctg ccattttcag tcttatttct
      241 ttatattccg tgccatttat aaagacatca aaaacgcttc cgccgggacc gcattccaat
      301 atccggtgct ttgttctttc cgcatcaaaa atactgctcc ccttttgacg ggccacctct
      361 tgaagcactg cctttgcttc acctttcaca ttaataacaa caggtacacc tgattttatt
      421 attccggctt tttcagaggc tatttccctg atggtgtccc ccagtctgtc accgtgatca
      481 tgggaaatag acgtgatcac cgatatcaag ggatttttta tgatgtttgt ggagtctccc
      541 ctgcctccga gtcccacttc cattaccaca atatccggtt tcattctggc gaaatacaaa
      601 aatgccactg cagtcagtat ttcaaattcc gtaggagaat catatccgtt ttccaccatt
      661 acacgggatt ttgctatcac cagatcagta cagacctcca gctctttttc ggggatcctt
      721 tccccgtcat attcgatcct ttcggaaaag tcctcaacaa aaggcgagtt atagaggcct
      781 gccctataac cgttagctct cagcacctca tatatgaatc tgcagacaga gcccttcccg
      841 ttggttccgg ccacatggat gatcttcagc tcttcctgag gatctcccag aagttccatg
      901 agtttttcta ttctttcaag acccagcaca ctcccgaatc ttttgaattt tgctattttc
      961 ttttctgcat tcattttttc tttttgcttt ctgtataggc aagcctttca caaactttgt
     1021 caaacataga tctgtattta accagttttt ctttttcttt tgatatgact tcttccggtg
     1081 ctttgctgac aaatcccggg ttagccagtt tcttctctat tcgctgcacc tcatttttta
     1141 acctgtcctt ttcttttacc agtcgcaggg tctccgcatc ataatcaata agttcatcgg
     1201 aaggaatgaa tatttcagcc cctcccgtaa ctgaagacat ggaatcttcg ggaatatcct
     1261 ctttgtcctc aataaatgat atattcgtta tatttgccag cttttttatg tacctttcac
     1321 ctgctctggc ggtttgctgc aaatgtccgt aagacagtat aacaaggctc agtttctttg
     1381 aagggggagc ttcaacttct gctcttatat tccttactgc ccttattatt tccatggcca
     1441 tttcaagctt ttttgtttct tccggataaa gttctttgtt ttcgtaagaa ggccagtggg
     1501 acttcataag gaacttcccg ggatcttcat ttgtgttccc gttgcccgga agagcacccc
     1561 atatctcctc ggttatgaaa ggcataaagg gatgcaggat tttgagcata tctttcaata
     1621 catggatcag tacgcctctt acaacttgtt tgtccccttc atcgctgccg taaagccttc
     1681 ctttggctat ctcgatatac cagtcacaat attggttcca tatggcttca taaacttttt
     1741 ggcccgcctg acccagatcg aatttttcca tggtttctgt gacttttttt attgtatcgt
     1801 ttgtggccga tatgatccat ttgtcttcag gctgaaggtg cctttcgctt tcagcatcta
     1861 tagtcgccac aggaaggaat ttgccttctt catctttgat gttcattatg acgaatcttg
     1921 atgcattcca aagtttgttg gcaaagtttc tggccatttg aagacgttct tcctggaatc
     1981 tcatgtcatt ccccggggtt atgcctgtgg ccagcataaa tctcaaagca tccgcaccga
     2041 actcatgtat gacttcaagg ggatcgattc cgtttcccag ggacttgctc attttacgtc
     2101 catcatgatc tcttacaagt ccatgtatga atacatgttt aaaaggaacc tctcctgtta
     2161 tctccatagc ggaaaaaacc atccttacta cccagaagaa tatgatgtca tatcctgtta
     2221 ccatggtcga tgtgggataa aaatatctca agtcctttgt gtcatcgggc cagcccaggg
     2281 tcgaaagggg ccacaatgcc gatgaaaacc atgtgtcaag gacatcttcg tcctgatgca
     2341 tccttttgct gccgcatttt tcacagcttt caggcttatc ggcggcaaca tttatctctc
     2401 cgcagtcttc acagtaccat gccggtatcc tgtgacccca ccagagctgt cttgaaacac
     2461 accagtctct tatgttttcc agccaatgca gatatgtttt ttcaaatctc tccggcacat
     2521 gtatcagttc accatccttt gccgctctca tggccggctt tgccagttct tccattgcca
     2581 cgaaccactg gtcagaaagc atgggttcaa ctgcagtacc acacctgtag catcctccaa
     2641 tgggtatttc tatttcttct gtttttacaa gatatccacc cttttcaaga tccgcgaccc
     2701 attcctttct gcattcgtat ctgtccattc ctttatattt tcctgccagc gagttcatct
     2761 ttgcattctc atctatacag gataatcttt ccagactgtg tctttcgccc acttcaaaat
     2821 cagaaaaatc atgtgccgga gttattttta cggcacctgt tcccttttcc ggatccgggt
     2881 aagtatctgc tatgaccggg atctctctgt ttactatggg aagaaggact tttttgccta
     2941 ccatgtcctt gtatctttca tctgacggat gcactgctat cgccacatca ccgaacattg
     3001 tctccggtct tgatgtggca acgaccatat cttttccccc atcctttgac ggatatctaa
     3061 agtaccagta agcacccttt ttgttttcat gctctacttc ggcatccgaa atagatgtac
     3121 cgcactcagg acaccagttt atgagacgat tgcctctgta tatgagcccc ttgttgtaaa
     3181 gacgaacaaa aaactcattt actgctttgt tgcatccctc atccatggtg aaccgctctc
     3241 tgctccagtc acaggagtca cccagttttc tgcattgacg cgttatttta ccgccatata
     3301 cttttttcca ttcccatgct cttttcagga actcttctct tcctatctct tctttttctt
     3361 tgccttcctc ttctctgatt ttttctacta ccttcacttc tgtagcaata ctggcatggt
     3421 cgcttcccgg gagccagaga gcagaaaacc cctgcattcg cttccatctt gtgagtatgt
     3481 cctgaagagt ctgatcaaga gcatgcccca tgtgcaactg gcctgtgata ttgggcggtg
     3541 gcataactat agtataagga gttttgtccg gatctctttc agctttgaaa gctccgcttt
     3601 tttcccatct ctcatatata cgctcttcaa aatcctttgg gtcataggtt ttggcaatat
     3661 tgatttcttt ttctatcatt atatctgctc cttttttata aaaaaatgtc cttttaatgc
     3721 tttttgcaat aaaaggacga aatattttcc gcggtaccac ctttattcca tacatatcag
     3781 gataatacat atggctcttt aaactgcaag gctcacaggt aaccttcgga aaattctttt
     3841 cccggaaatc tttcagccac ggatctcctt ctctgatggg cagttttttc cctactcctc
     3901 ccgctcttcg ccttttattc gattatgaag taagtatagg gcatttcaat ggattggtca
     3961 agaattttgt cgctgccgcg cattttttat agccggtttt tgttttcaaa aaaagcagct
     4021 tattatactt agtcacttgc tctaaaaaag tgctttgaat ctttcagtga tgccctctat
     4081 gccattcttg atccccgcac ctttgcccgg ggacctttta tctgcagtgc cacattttga
     4141 acagttatct tttttttcta tgccgtctac cctgattaca aactgcacca tactgacttt
     4201 tgtatttttc tttgaaacaa aggatgtggc tctgtaatct gatttgtcat attcggcaat
     4261 gacactttct atcttttccg ctatcaaaga aggtatcccg gatgttttct gatacagccc
     4321 ggttgcgcct tcttttagtg ccgatgaccc cttggtactt tccgatatcc cttcagtata
     4381 cgaaagcagt cccgaatgga aaagcgaata gttttcattc agaagggata tattctgctg
     4441 aagctcatct atgccttccc cggctgcccc aagagccagc gaagatgagg caaagacttc
     4501 tgaaacatca gcactgccat aaatgctttt tactatctgt gctttgtcat aggtgtctat
     4561 cagcgtattc aggtttgtta tatcctcttc cgtggcctga ccgttatctc tggcatatgt
     4621 taaggcccct atcaggtcat ccatatcctc ctgtccaagg tcatctgccg gtatatcgtt
     4681 tatcgcttct cccaaagtgt cgtttgcgtt atcaagagca tcggccaata agccaagggc
     4741 ctccgcatca aacccaccct gtccagctgc cagggaatta tttattattt gcagtgcact
     4801 ctttattttt tcagaactgc caattagctg tgaagaaccc gcgtcgattt ttttgagtcc
     4861 tttctcgaat tctctgactc cctgctccag ttgtcctatg ccgtatacga tggatgtcat
     4921 acccttttcc agtgatgaca tatccggtat ttcaatacca actgaaaaag gcagtgctgt
     4981 tatgtctatg ccttccatgc tgaaagccga agaatccgtg gtgagtgata cgatatcttc
     5041 cttgccgggc atgattgaga atgatacgat tttatcattt cccgaattgg caaaagtccc
     5101 tcctgatgcc tttatttttt tacatttttc tgcatccagc ttaatggata tctgcagcat
     5161 atagttttca aaatatgagg ggtcgacatt ttggtttttt tctgttgtga tctgtatcct
     5221 tacattgcct gtagcgccgg aaagagcgcc cggactgacc gtctccccat taagaaaata
     5281 cttgacgtct atattccagg ggaggtcatt tttctttatc tggcctttat aataaaatct
     5341 gccttcacct acatcggctg ttatcattcc tccgttattt ttgatctttt ttgcacttgt
     5401 cagatttgtt acagaagaat aatctccgta atctgttatt ttccctgccc tttctgtctc
     5461 aaaaatattc accgcatata tgtctttcac atttccggaa acagaaagat cgccgtaaat
     5521 gacttcttcc tttggtgagg gttttgctcc tgttatctgg gcgatcacag gcacagacat
     5581 ggttatcaga aaaatcacca ttgtgaaaaa caccgttata agcatatgtt tcttatgtat
     5641 tttacttttc ctccggatca tttttcactt ctcctttttt aaaaaatctg atttataagt
     5701 agtggctccc aatggtttgt ccagcatcat caagagggcc ggcaggaagc agactacaat
     5761 tatcaatgac agtatcgctc ctcttcccaa aaggatcccc atttctgcaa ccatctgatt
     5821 gctggataac gctcccagta cgaatcccgc cgaagacagt attacggctg ataacagtat
     5881 ggacctgaac ctttccgcca gagactcttt cattgcctct tttggataca tgctttttct
     5941 ttttttctta taagtgtctg tcagcagtat ggcatagtcc accgtggctc ccagctgcac
     6001 tgtattaact ataagataac ctatataatt catgggactg ccttcgaaat aagggatggc
     6061 aagattcacc catattgccg cttttatgga aagcaccagc aaaaccggaa taagaagaga
     6121 cctgaaggtt atcatgataa caagtgcaat ggccaataca gcaataatat tcacccttct
     6181 attatccagg gtgaccacat ctttcatgtc tcttaaattg gcgctctccc cgcaaagata
     6241 tacatttgtc ccatagtact tttctgccgt tcttctcacc tcgtcaactg tggtgaatgc
     6301 ctcttcccct tctgcggaag tactggtata tacaactatc ctggaatgat gttttgaata
     6361 aaagcccgat gctatttctt cacctatata ttcatcgggt atctgtgctc ctactgaaga
     6421 aacatatgaa accacttctg ttacattttc ttttttttca agttcgctgc aaagctgtac
     6481 ctctttcccg acttctcctt ttggcagaag aataacgata gccgttgagt ctccgaaaac
     6541 ttcttttaca gatgcttcgt cctctcctgt cctctgccct tcaaccaccc cggtactttc
     6601 ataaaggaat tcattgctgt tttgtgccag ccagcaggga tatattatta tagccattaa
     6661 tatcagactt gctgtactta ttttcataaa ggccctgctg gaacccgaca tctcaggcag
     6721 tatcctttta tgctttgttc ttttgatcat cttgagacag caaagcgtaa gggccggcag
     6781 gaatacaagg accgaaacaa aactgaatat tattcccttt gccaggttga atcccagatc
     6841 agctcctatt tcaaaatcca taaatgtcag agcaacaaat ccgaaaaggg tggtggcaga
     6901 gctggcggca atggcgggga aggatttttt cattgcggct atcattgctt cttcaggatc
     6961 atctttttgc ttttgcattt cggaaaatcg gtgcaaaaga aatattgcat aatccaggga
     7021 aactgccagt tgaagtatcg gtgtcacgga ctgggttata tatgacacct caccgatcaa
     7081 aaggttcgtc cccatattta ttattacgga aactccaatg gcaagaagaa aaagaatcgg
     7141 ctctatccat gaagtagtgg taagtatgag aagcagcaat atgatcggca acagtataat
     7201 tgtagcttta agcgtttccc acaccgtggc cgtctgtacg ctggccagag ccagagcatt
     7261 tccctccagt gcatttcttt ctccgataac ttcatatacc tttgaggtta tttccagttc
     7321 cctgccctct tcaactgcga atgatatgag ggcatttccg tttttatagt atttgtcgct
     7381 tgtttctttg tctgctattt caagaggctt ttttatatca gaaacatcat caagccatat
     7441 cacatcagat ataccgtcta tgttttcaat ttcattttta tattccaggg cttctcccac
     7501 tgtaacattt tttagcatca ctctggcatt gggaacagcc tgatcaaatt cattttccat
     7561 tattttcaga gcttttgtcg actttgattc ttccggaaga tagtcggtga gatcataatt
     7621 gacatacact ccaaaaaaag aaacagcaca tatggcagta aatattataa atacaacaac
     7681 cacaaatttt ctttttttta ctatgatcct gccgatatgt tccattaatg tctttccttc
     7741 tgccgggact tgacctttta tatatcttta tatcacttcc atatagtctt tatattatat
     7801 gccatattta taataataca atgataaaat cactttaata tatgtatttt tagaccgttt
     7861 cttcctgtat ctcttgtgcc ctttccgata ccggttttat atatatcttt gtcctccggt
     7921 ttttttctgt attaggcccc tgaacctttc aatataccca gccctcccgg tgttaccatt
     7981 tctgtttcta tttcatcttg gacaaatctg taccccgttc ctttcatcat ggcacggact
     8041 gcaggcaccg gcaccggtat cctgccatgg tcgcagtcaa tatggccaaa tccgtcatgg
     8101 atctcagaac agtaaatatt atcgggataa aggtttgaca atgcagcaga aactcccact
     8161 acatttatta tcgccctgtc tcttcccagt tcatgaaaat gtacggtgtc catgcttttt
     8221 ccgtgaactt ccgcctctgc ttctgctata acagtatata ttttgagtgc ccttctcttt
     8281 gtattatcat caagaccgct ttttctcaaa atatcttcaa tttccctgaa gcttcttttt
     8341 gtattatggt cacatagatt cttttttttc ttttttacta tttcattcgg tctttcacat
     8401 atattcagtt tgtctgtctc ccgcaaaaca aaatctctgt ctgctcccag gtccagcaga
     8461 gccagaagca ccatgtctgt actgattccg ttgctgcagt caaaatatag atccattttt
     8521 ttgtcctctc cgtaaaaacg ggcatgcatt actgcatgcc cgcaaaaaag atttagtgtt
     8581 ttttagtcaa tatcatctat cttatcagca agagatctca ttgaggatac ttccttggat
     8641 atctcagctt tcagatctac aagtgcttcg tcaagagccg ctttatatac ttcttttgct
     8701 gctttgtaat ctatcttttc tcctgcggca tcctttctga ccagatcata ggtggtatcc
     8761 cctacccagt aaaccggcgg cagcttgtct gtaaagaact gtcttttccc tgtccattta
     8821 gtatcgtcat actgtataag ggtctgtccg gccacttctt tggagaacag atagtagttg
     8881 taatccattc cgccgttcaa ttgccaggcc atatccagcg ctccgttttc tttttttgtc
     8941 caggtctttt gtttgtccag tccatccatt actccctgaa ggatacctat gttattgtct
     9001 accagggcat gtcttatcgc cagtccccag gtatctgttc cgataagttc tttctggatc
     9061 tccttataaa cttcccttac ctgcttgtta agttcagcac cttcttctct gcactgcttt
     9121 atgccctctt catcttcttc cgcaacaagt ttttcgtaat ttacatttac ctgctgcatc
     9181 ttatcataaa gtacctcgca tgcttcacga aggtcagcaa gttcagcctt gtatccttct
     9241 atatccgcat ccgcttcttt ggctgtatct tcattcagag cttcttccaa atcatcgcat
     9301 gtagcacgaa gatccagttc catggccggc attttgtcag tatatacaag cattgctccg
     9361 tagaagtttg catttgaaac atatacatcc ttgtcccatg tatcctttgt atcgaactgg
     9421 gtatgatatg tagtggccga gaaatctccg ctctggaatc cattaagtat atatggaact
     9481 cctgcccacc tgtatgaaac accgtcttcc attgtgtcag agtctaccgt tttagccttt
     9541 atcctcttta tatgacctga cctaactacc agaccggaat catcaacaaa gtttttcaca
     9601 aaagtactta attcgggaac tgttacagca aatatcttat ctgttccgtc atcttttgca
     9661 ttcagttcaa aattgaaaag acttttggtc tttgcaggcc attcgggctt gccccgggta
     9721 accattcccc aagctccggt ggtccagtca tcctgagcat ttgaaagtcc ccattcttcc
     9781 gaaccatgac aaacaaagac gagatcgttt tcaggttcat agcccgaatc tttaagggct
     9841 ttggccattg aaagaacggt acctatggca atgctgtcat cctggaatcc gaaccagtat
     9901 ttatcataat gtgcagaaac gatagtctgc tgatcggaat cctttccttt tattttgccc
     9961 actacattat aagtggtgcc tgttcccttt tctacaactg catctatcat aagtgtcgct
    10021 tttttgttac cgtcttttat tgcttttttg atcttctttg cttcattggc acttatggca
    10081 gcagtgggaa ggagatcatc acagcataca tcctgaacat ttattgtgtc tttatttagt
    10141 tctccgtatc cactgtttgc ataagacact actgctgccg ctcccttttg ataggcctgt
    10201 cttatatagc cgtctatcca tgattcatta gcctgatcta cctgaaccag cactattttg
    10261 ccggccacgt ttttgccttc ataatcagct tcaaatcctg tcttgcagtc aactatttct
    10321 gctgttatgc catcttcccc ggtaccggtc tgctgatatg aggccggcat aagatttatc
    10381 ttcgttcctt caacagtcag tttggaatca ttgaactgga acttgtcaca aggtacaccg
    10441 accttttcaa catcttcaag tccgatattt tccatttctt ccatcagata atcggaacat
    10501 gcgtgttccg catccgaacc cgcacttctc catccaagct cattgtcaaa atactcttcg
    10561 tcataagcaa gggttttggc tatcccataa gcatatttgg agtccacatt atccaagtaa
    10621 tcctgcacgt ccccggcgaa tgaataatcg gaagtgattt ttgtctggaa ggtatcttcc
    10681 tttgcctcct ctttctcacc gcatgctgca aacgtccaaa ctaccatgga taaagcaata
    10741 gcgatacaga ccaacttttt gaatttcatg ctaacctcct tataaataaa atataactac
    10801 aatattaact tttttcactt ataaatgtat ccttttattt ccttttcata aaggtcgtaa
    10861 tcatcatcgg gaaccggctg taagtcaata ttttcgaatt cttccctatg ggtgtcatac
    10921 tgatcgtaaa atcccagctc agctctgtag acttcaccgg tctccgtatc ataaagacgg
    10981 tcatagccca tggtagcatc gcttcttttc tgtgaaagag catcattgac tctctgcctg
    11041 tttgaccatg cttcgttata tgagtctgcc gcttccgaaa gagtgcggcc cacctgcagt
    11101 gccatttcag ttccccagcg gctcctggca actccctgct ctacatatct gtctgtatat
    11161 ctaaagcttg acaggctttt gagaagagtg tccttgagct gataaaattc atcggccgga
    11221 gcgcttattc cggttatgac atatacggta tagtaacccg catccacatc atacatatag
    11281 ctggtcatgg tatctgccac acctgccgca aaaagacctt cgcagggtat gtcatcattc
    11341 ttgaacaaaa ccctcagtat ggaatcttca atacagtttg aggcgatcgg cgagttcctg
    11401 ggtgtggtct caaggatctc cagatcatta aaaacaggaa aatcatgatc aattccatat
    11461 ttttttgcgt atgatgtaaa ttcatcaaaa gtattgtaaa actcctctgt ggtagccggc
    11521 gaaagcacgg gtgcatccgc atatactccc gcatctgcat atcctccctc ggccatatac
    11581 ctgttccatg cttctttccc ttcactgctt ttcatgaaat acttcatgtt gccgtaaaaa
    11641 aatatctgcc ttgaaggttt atccggatca tatgcacgaa aaccgaaatt ttcatattct
    11701 cctaccgtat gtatctccca gccctttggc agcttcatgc taaaaaaatc tccgtcataa
    11761 gactgaagct tagcattgac ttcgggtgcc ggatctatct tgagttcgtt tccgccagtt
    11821 gaagaacaag cagaaaaaat catagcaata atcaataccc ctaaccacaa tatacttttt
    11881 gttctcatag tattctcctt tttacataca aaacattttc ggtatatact atattaaatc
    11941 actaatttca gaatatttca ataaatatta tgctgttttt tcacgtcaat tttgcgttca
    12001 ggaaaaatgg ctcttcccca gcattcaacc ag
//
LOCUS       DAONHK010000025        11768 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_4263_length_11768_cov_5.074618, whole genome shotgun sequence.
ACCESSION   DAONHK010000025 DAONHK010000000
VERSION     DAONHK010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 11768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11768
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4263_length_11768_cov_5.074618"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /gene="atpH"
                     /locus_tag="PLM92_01770"
     CDS             <1..540
                     /gene="atpH"
                     /locus_tag="PLM92_01770"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HPR23899.1"
                     /translation="LRMAELTIDMTYGSALFQVAQDLKKEKKILEDAEGVLSVLQKEK
                     ELNAFIDNPTVPAEEKKEAIKKIFEQNICKELLNLLYVLVDKRRTRHYPKIIRYYKGL
                     VNKKEGFAYGTVFSVNPLTKKQIKDFESKTEVLMNSRVKLENITDASLIGGVKILING
                     KIIDASLRKRLNDLENILK"
     gene            556..2238
                     /gene="atpA"
                     /locus_tag="PLM92_01775"
     CDS             556..2238
                     /gene="atpA"
                     /locus_tag="PLM92_01775"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015923501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HPR23900.1"
                     /translation="MNLRPEEISAVIKEQIKNYENQLDVSDFGTVIQVGDGIARVYGL
                     DNCMSGELLEFPGGIYGMALNLEEDNVGTVILGSDKEIKEGDIVKPTKRVVEVPVGEE
                     LIGRVVNSLGQPLDGKGPIKTGRTRPVESPAPGVLQRQSVKEPLQTGLKAIDSMIPIG
                     KGQRELIIGDRQTGKTAIAIDTILNQKDEDVICIYVAIGQKKSTVAQLVQALESKGAM
                     RNTIIVSATASDVAPLQYIAPYAGCAMGEYFMYMGKHVLVIYDDLSKHAVAYRAMSLL
                     LRRPQGREAYPGDVFYLHSRLLERAAKLSDELGGGSLTALPIIETQAGDVSAYIPTNV
                     ISITDGQIFLETELFFTGQRPAINAGISVSRVGGDAQIKAMKKVASKVKLELAQYREL
                     ASFSQFGSDVDADTKARLDHGERLMEIIKQGQYKPLPVSHQVMIIYAAVNGYLKDVPV
                     ESIQAFEKAFYEHMDTHHPQIGKELVAKGELSKENESALVSALEDFKRITDFSVKPEN
                     PVLEKEIGDDSDEQCVCEPGADEKTSKKALKQAAKAEKKAAKEAKKADRKKE"
     gene            2242..3108
                     /gene="atpG"
                     /locus_tag="PLM92_01780"
     CDS             2242..3108
                     /gene="atpG"
                     /locus_tag="PLM92_01780"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HPR23901.1"
                     /translation="MASSDMQDVKRRIKSVTSMEHITNAMKLVSAAKLRRAKATFEKT
                     NEYFHFITQSIAEVFNNASEVPDKYLTGNREIVNTCYIIVTSCRGLCGSFNSNIIKEA
                     ETEMKSDPEKPVIVAIGTKGRDYFAKRDYEIKNQYLAPPESISFLETKQISEPIIEMY
                     NSGEIDEVVLIYTSYVSTLEQRVKNVTLLPFEMEKDPDIMRYGKEVEYEPSVEEVFNY
                     LVPKYVEIMIYGAVVESATCEHAARRMAMENATDNARDMLSDLSLFYNRARQSAITDE
                     IIEIVAGSEAQK"
     gene            3122..4519
                     /gene="atpD"
                     /locus_tag="PLM92_01785"
     CDS             3122..4519
                     /gene="atpD"
                     /locus_tag="PLM92_01785"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HPR23902.1"
                     /translation="MNKGIIHQIIGPVIDIKFDPEKMPELLNAINIPFGEKKIVAEAA
                     QHIGDDVVRCVALSSTDGLTRGMEAHDTGGPIKVPVGKEVLGRLFNVIGETIDDKGPV
                     NTKETSSIHRPAPDFEEQDTSSNIFETGIKVIDLIAPYTRGGKVGLFGGAGVGKTVLI
                     QELISNIAKEHGGISVFAGVGERTREGNDLYYEMIESGVIEKTAMVFGQMNEPPGARM
                     RVGLTGLTMAEYFRDTEGQDVLLFIDNIFRFTQAGSEVSALLGRVPSAVGYQPTLATE
                     MGALQERITSTKKGSITSVQAIYVPADDLTDPAPATTFAHLDATTVLSRAITELGIYP
                     AVDPLDSSSRIMDPLIIGEEHYNTARGVQEVLQKYKDLQDIIAILGMDELSDSDKLVV
                     NRARKIQRFMSQPFSVAEQFTGTPGKYLPLKETVRGFKEILEGKHDDIPENLFLFAGG
                     IDEVVERHEKGNKNG"
     gene            4512..4943
                     /gene="atpC"
                     /locus_tag="PLM92_01790"
     CDS             4512..4943
                     /gene="atpC"
                     /locus_tag="PLM92_01790"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HPR23903.1"
                     /translation="MARSIKLDVITPSKRFYSGDTELVIVRTLLGDEGFMAGHSWACK
                     LLDYGELWIKEAGERDFRVAAVSGGFVDVKDSIIIYTDAAEWSDEIDTERCMNMKEMA
                     ESWLCSEEEHDPNDVTKAKIALTKAIVRMNVAEGGRRRKRP"
     gene            4956..5858
                     /locus_tag="PLM92_01795"
     CDS             4956..5858
                     /locus_tag="PLM92_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPR23904.1"
                     /translation="MKRKALFILFTAFMMSFILAGCGEDEDATEEKGTLSGNISIVAQ
                     KGAPTETLNALGSDYDITSYYDHISVEAKIADGDFDAAIVSTNTAAQLYEKTDGNVIA
                     VSPVVMGDLYIVSNGISIPDDGISFLRGKTIIGCCEGGTGEYALKKLLEDNYINTEYG
                     ISFEWVSRPQDVKEALKEPYTVALLQEPYASEAAKSIKNAEKAVELDKLWEETYNSHI
                     PTDVLVVSRSFAEKREKDLKIFLAEYEDSLDKAIENTKSKLVLYARSNRGEMLIKNYI
                     NVMEEFDLESLGGKAPKSDFYYGI"
     gene            5886..6215
                     /locus_tag="PLM92_01800"
     CDS             5886..6215
                     /locus_tag="PLM92_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPR23905.1"
                     /translation="MAGPISIKLKETISINELAELVEKNWNEEKYGKASLVEFRDKPY
                     IHLDGSDWFNVLVYPGRKKVTVQSLPENGQIVLTKEGKKQMKMVNKEILPKVAEGFRE
                     ILSDILK"
     gene            complement(6208..7146)
                     /locus_tag="PLM92_01805"
     CDS             complement(6208..7146)
                     /locus_tag="PLM92_01805"
                     /inference="COORDINATES: protein motif:HMM:NF014609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HPR23906.1"
                     /translation="MKFYIVDAFSSAAFGGNPAGVVIIPEGDDFPSASLMQKTAAELR
                     YSETAFVKRLNEKEFHTRYFTPAAEVDLCGHATIGAFYVMLKEGILSKEQKYMNHTLA
                     GDLEISAQKDFIMMEMAIPEHIKTISGKTETETLFDIMGIPVGEVFIHQSPGDKKKLL
                     PMIISTGLPDILLPVSDRNTLFDIDPDFDALSRFSEEYEVTGVHAFTLDTAEDDITAH
                     CRNFAPLYDINEEAATGTANGALTYYLFLHDLVKDGQDSVFIQGESMGRPSRILSHIK
                     KVPRYENNRDSKKDDFFLKIQIGGTGMILAAGEIFI"
     gene            complement(7143..9221)
                     /gene="feoB"
                     /locus_tag="PLM92_01810"
     CDS             complement(7143..9221)
                     /gene="feoB"
                     /locus_tag="PLM92_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HPR23907.1"
                     /translation="MNKSRKGNENRRRRRHHDKKSDTVSVAIIGNPNCGATTLFNRMT
                     GGIKQRGRFFECCHTAKEGPVRAHKNVTVVELPGTYSLSAYTLNDITARDILINGKPD
                     VIVNIVDATGFERSLYLTLQLMELDIPMVVALNMMDEVRNNDIDFDLQKLEKELTVPV
                     IPISASKNRGIHDLIHYAIEAAKDKTVPEKIDFCSGHVHTAIHSIAHLIKDDAEKADL
                     PLRFCCTKIVEGDEPVLKQLDLKDPELKIIDDIISDMEHHLNTDREAAMADMRYAFIQ
                     DLAAKVITRTASTTREQDRSLIIDKILTHRIFAIPIFFGIMLLIFYFTFDSIGQWLSD
                     WLGMGIDKTVSLIDNGLSVSGVAPALHSLIINGVCAGVGSVLSFLPRIAVLFFFLSIL
                     EDSGYMPRVAFVMDKLLRKVGLSGQSIVPMLIGFGCSVPAIMATRSLPSKRDRHLTIA
                     LIPFMSCSAKLPLYAMFISAFFEDNRVFIMAAIYLMGIIVSVLCALIFKHTIYKGDPV
                     PFVLVLPPYRLPAMKSVWLRMWENVKEFIKKAFTIVFIATIVIWFLQSFDAGMNMVSD
                     PDDSILAAIGKVAVPLFEPLGFADWRASTALITGLSAKEAVVSTMAVLTGATDGAALS
                     AMLIEIFTPLTAFVFMVFCLLYMPCVATLATIKKEMNGWWHAILVVLFQTTVAWIVAF
                     IVYSVGGIFI"
     gene            complement(9214..9507)
                     /locus_tag="PLM92_01815"
     CDS             complement(9214..9507)
                     /locus_tag="PLM92_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HPR23908.1"
                     /translation="MTLDMLKPGARARITAVKGKGALRRRLLDLGLTPGTIVMIRKVA
                     PLGDPVEICLRGFELSLRKAEASEIEVAAEDCSENTNCNICPYSKPEDKTSDE"
     gene            9676..>11768
                     /locus_tag="PLM92_01820"
     CDS             9676..>11768
                     /locus_tag="PLM92_01820"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HPR23909.1"
                     /translation="MKKKYIIKGMGCAACAATIEKSVNAMPSVREARVNLVSDSMIID
                     FDEKQTGSSEIIKTVRESGYDAEETKSTYECKKNEEKTAVREIKELKYRFLISLIFLI
                     PLMVLSMGAMINLPVPEVSYWILGVLAVPVAAINYKYYTRGFKLLFKASPNMDSLIAI
                     GSLAALILGYFESAVMILTLVTLGKWLEARSKGRTGDAVKKLMALAPEEARIITKDGQ
                     EKIIPAEDLLPGDIIAVKSGESIPADGTVTEGTCTLDESTLTGESMPVDKGPGDEVFT
                     ATINKAGYLRMKADKVGEETAFSGIIRLVEEAANSKAPIARQADKVSAFFVPAVILIA
                     VITAVCWILVGAEADQVGSMSIAVLVISCPCALGLATPLAIMTGTGRGAEKGILFRTA
                     EALETVSKTRVAVIDKTGTLTKGKPEISEIVTIKGTEEDVIAYTASLEKLSEHPLGLP
                     IIERAEKKGLDIKKVNKFLVMPGRGIEGIIDGKRVLSGNKEFMHENDIFTEEGRTAKL
                     YETGRTLIYTGIDGDLAGIIALRDEPKESSREAVLELKEMGIETVMLTGDNQMTAEVI
                     AKETGIDKVVAQVMPEEKKNVIDDIKKEGKSVLMIGDGINDAPALKTADTGIAIGAGT
                     DIAIEAADIVLMHEDPKDGASAVKLSKAVVRNIKQNLFWAFFYNSLGIPLAAGVFYPF
                     TGWTLTSWTASSRSG"
BASE COUNT         3775 a         2277 c         2835 g         2881 t
ORIGIN      
        1 ctcagaatgg cagaactgac cattgatatg acatatggaa gtgctctgtt tcaggtggcg
       61 caggatctga aaaaagagaa aaagatcctg gaagatgctg aaggtgttct ttcggtactc
      121 caaaaagaaa aagaacttaa cgcatttatc gataatccca cagttccggc agaagaaaaa
      181 aaagaagcaa tcaaaaaaat ttttgagcag aacatatgca aggaactgct taatcttctt
      241 tatgttctgg tggataaacg gagaacaaga cattatccca aaataataag atattacaaa
      301 ggtcttgtta ataaaaaaga aggttttgca tacgggaccg ttttctcggt caatcctctg
      361 acaaagaaac agataaagga ttttgaaagc aaaaccgaag tactcatgaa cagcagagta
      421 aagcttgaaa atataacaga cgcttccctt atcggaggag taaagatcct tataaacggc
      481 aaaataatag atgcttcttt gcggaagaga ctcaatgatt tggaaaacat tctgaaataa
      541 gcaggaggaa atcaaatgaa tttaagaccc gaagaaataa gtgcggtcat aaaagaacaa
      601 atcaagaact atgaaaacca gcttgatgtt tcagatttcg gtaccgtaat acaggtagga
      661 gacgggatcg ccagagtata cggacttgat aactgtatgt ccggagaact tctggaattt
      721 cccggaggca tatacggaat ggcactaaac ctggaagaag ataatgtggg aacagtaata
      781 ctgggatctg acaaggaaat aaaagaaggg gatatagtaa agccaaccaa aagggtggta
      841 gaggtaccgg tgggcgaaga actgatcggc agagtagtaa attctctggg tcagccatta
      901 gacggcaaag gaccgatcaa aaccggcagg acccgccccg tggaaagccc ggccccgggc
      961 gttcttcaga gacagtcggt aaaagagcct ctgcagaccg gtcttaaagc aatagattca
     1021 atgataccca taggtaaagg tcagagagag cttattatcg gcgacaggca gaccggcaaa
     1081 accgccatag ccatcgacac catacttaat cagaaggatg aagatgtcat ttgcatatat
     1141 gttgcgatag gccagaaaaa atccacggtg gcgcagctgg tacaggccct ggagagcaag
     1201 ggcgcaatga ggaacactat catagtatct gccacagcca gtgacgtggc gcctcttcag
     1261 tacatagctc cctatgcggg atgcgccatg ggagagtatt tcatgtatat gggaaagcat
     1321 gttcttgtta tatatgatga cctttcaaaa catgcggtgg cttatagagc catgtcactg
     1381 ctgcttagaa gaccgcaggg aagagaagct tatcccggag atgtatttta ccttcacagc
     1441 aggttgctgg aaagagcggc aaagctttca gatgaacttg ggggaggttc tcttacggct
     1501 ctcccgatta ttgagaccca ggcgggagat gtttcggcat atataccgac taatgtaata
     1561 tccattacag acggccagat attccttgag acggaattgt tcttcacggg gcagaggcct
     1621 gccataaatg cgggtatttc ggtatcccgt gtgggaggcg atgctcagat caaagccatg
     1681 aaaaaagttg ccagcaaggt aaagctggag ttggcccagt acagagaact ggccagcttc
     1741 tcacagtttg gttccgatgt ggatgcagat acaaaagcca ggctggatca cggggaaaga
     1801 cttatggaga tcataaagca gggacaatac aaaccccttc ccgtaagcca ccaggtaatg
     1861 atcatatatg cggctgtaaa cggatatctc aaagatgttc cggttgagag tatacaggcg
     1921 tttgaaaaag ctttttatga gcacatggac actcatcacc cgcaaatagg aaaagaactt
     1981 gtagcaaagg gagaactgtc aaaggaaaat gaaagtgctc ttgtaagtgc ccttgaagat
     2041 ttcaagagga taacagattt ttccgtgaaa cccgaaaatc cggtattaga aaaagagatc
     2101 ggcgatgatt cagatgagca gtgcgtatgc gagcccggag cggatgaaaa gaccagcaaa
     2161 aaagcattga aacaagccgc aaaagccgaa aaaaaagcag caaaagaagc aaagaaggct
     2221 gacaggaaaa aggagtagca aatggcatca agtgatatgc aggatgtcaa aaggcgcata
     2281 aaaagtgtaa ccagcatgga gcatataaca aatgcaatga agctggtttc ggcagcaaaa
     2341 ctcaggagag ccaaagcgac ttttgaaaaa acaaatgaat attttcactt cataactcag
     2401 tccattgcag aggtgttcaa taatgcctct gaggtacctg acaaatatct tacaggaaac
     2461 agggaaatag taaatacctg ttatattatt gtaaccagtt gcaggggtct ttgcggaagc
     2521 ttcaattcaa atataataaa agaagcggaa acagagatga agtccgatcc cgaaaaacct
     2581 gttatcgtgg ccattggaac caagggcaga gattattttg caaaaaggga ttatgaaata
     2641 aaaaatcagt atctggcacc gcctgaaagc atttcttttc ttgagactaa acagataagt
     2701 gagccaataa tagaaatgta taattccggg gaaatagatg aggtggttct aatatataca
     2761 tcatatgtca gtaccctcga acagagagtc aaaaatgtga cccttctgcc ctttgaaatg
     2821 gaaaaggatc cggatatcat gagatacgga aaagaagttg aatatgaacc ttcggtagaa
     2881 gaagtattca attacctggt gcccaaatat gttgaaataa tgatatacgg cgctgttgtt
     2941 gaatcagcca catgcgaaca tgcggccaga agaatggcta tggagaacgc aacagataat
     3001 gcaagagata tgttaagcga tttatcgctg ttttataaca gggcgagaca atcggcaata
     3061 acagatgaaa taatagagat agtagctggt tcagaagctc aaaaatagga ggtagttcaa
     3121 ggtgaacaaa ggaatcatac atcagataat aggtcctgtt atcgacataa aatttgatcc
     3181 ggaaaaaatg ccggaacttc ttaatgccat aaacataccg ttcggtgaaa agaagatagt
     3241 cgctgaagcg gcccagcata taggagatga cgtagtaaga tgcgttgcac tttcctctac
     3301 agacggtctt acaagaggaa tggaagccca cgatacagga ggtcccataa aagtccctgt
     3361 gggcaaagaa gtactgggaa ggctttttaa tgtaataggt gaaactatag atgacaaagg
     3421 acctgttaat acaaaagaga catcttcgat acacagaccg gctccggatt ttgaagagca
     3481 ggacacatcc tctaatatat ttgaaacagg tataaaagta atagatctga tagctccata
     3541 tacaagaggc ggtaaagtag gtcttttcgg aggtgcgggc gtaggcaaaa cagttcttat
     3601 acaggagctt atcagcaata tcgccaaaga gcacggcggc atttcagtat ttgcaggggt
     3661 gggagaacgt acccgtgaag gtaatgacct ttattatgaa atgatcgaat ccggtgtaat
     3721 agaaaaaacc gcaatggttt tcggacagat gaacgagcct cccggggcaa gaatgagagt
     3781 ggggcttaca ggccttacaa tggctgaata ttttagagat acagaggggc aggatgtact
     3841 tctctttata gataatatat tcagatttac acaggcaggc tctgaagttt cggcattgct
     3901 gggacgtgta ccttcggcag ttgggtatca gccaactctt gctacggaga tgggggccct
     3961 tcaggaaagg ataacttcta cgaaaaaagg ttcgataact tcagtacagg ccatatatgt
     4021 gcctgcagat gacttgactg atcccgctcc ggctacgaca tttgcacatc ttgatgccac
     4081 tacagtactt tccagagcta taacggagct gggtatttat ccggcggtgg atccccttga
     4141 ttcatcttca agaataatgg atccccttat aataggggaa gagcattata ataccgcaag
     4201 aggagtacag gaagtgctcc aaaagtataa ggatcttcag gatataatag caatacttgg
     4261 catggatgag ctttctgatt cggataaact ggtcgttaac agagcaagaa agatacagcg
     4321 tttcatgtca cagcccttca gtgttgcaga gcagtttaca ggaacacccg ggaaatatct
     4381 gcctctcaaa gaaaccgtaa gagggttcaa agagatactt gaaggcaagc atgatgatat
     4441 accggaaaat ctcttcctgt ttgcaggagg gatagacgaa gtcgttgaaa gacacgaaaa
     4501 gggtaataag aatggctaga agcataaaac ttgatgtcat aacaccttca aaaaggtttt
     4561 acagcggaga tacggagctt gtcatagtaa ggacccttct gggagacgaa ggtttcatgg
     4621 cagggcattc ctgggcatgc aaacttcttg attacggcga attgtggata aaggaagccg
     4681 gagaaaggga tttcagagtc gctgctgttt caggtggatt tgttgatgtc aaggacagca
     4741 taataatata tacggatgcg gcagagtggt ctgatgagat cgatacagaa agatgtatga
     4801 acatgaaaga aatggcggaa tcctggctct gcagcgaaga ggagcatgac cccaatgatg
     4861 tgacgaaagc caagatagcc ttgacaaagg caatagtaag aatgaatgtg gctgaaggag
     4921 gtcgccgcag aaaaagacct tagggagatg atcctatgaa aagaaaggca ctttttatcc
     4981 tatttactgc atttatgatg tcgtttattt tggcaggatg cggagaagat gaagacgcaa
     5041 cagaagaaaa agggacactg tcgggaaata taagcatagt tgctcagaaa ggggccccga
     5101 cagaaacttt gaatgctctg ggcagtgatt acgatataac atcatattat gatcatatat
     5161 cagtagaagc taaaatagcc gacggtgatt ttgatgcagc catcgtatca acaaatactg
     5221 ccgcacagct ttatgaaaaa acagacggga atgttatcgc agtcagtccc gttgtaatgg
     5281 gagatcttta tatagtttca aatggtatca gtatacccga tgacggcatt tcatttttga
     5341 ggggcaagac cataatagga tgctgtgagg gaggtaccgg agaatatgct ctaaagaagc
     5401 ttctggaaga caattatata aacacggaat atggaataag tttcgagtgg gtatcaagac
     5461 cgcaggatgt aaaagaagca ctgaaggagc catatacggt ggcactgctg caggaaccct
     5521 atgcatcgga agcggcaaaa agcatcaaaa atgcggaaaa agccgttgaa ttggacaaac
     5581 tctgggagga aacatataat tcccatatac cgacagatgt tcttgttgtg agcagatcct
     5641 ttgcagaaaa aagggaaaaa gacctgaaaa tatttttggc ggaatatgaa gattcccttg
     5701 ataaagcaat agaaaacaca aaatccaagc tggtgcttta tgcaagaagt aacagaggtg
     5761 agatgcttat caagaattat ataaatgtca tggaagagtt cgatttggaa tccttggggg
     5821 gaaaagcacc gaaaagtgat ttttattacg gcatatagtc attaaagaaa aaaggagaat
     5881 tatatatggc aggaccaata tcaataaaac ttaaagaaac catatcaata aatgaacttg
     5941 cagagctggt tgaaaaaaac tggaatgaag aaaaatacgg gaaagcgtct ctggttgaat
     6001 tcagagacaa accctatata catcttgatg ggagtgactg gttcaatgtt ttggtatatc
     6061 cgggcagaaa gaaagtcact gtacagtctc ttcccgagaa tggtcagata gtacttacca
     6121 aagaagggaa aaaacaaatg aaaatggtca acaaggaaat cctcccaaag gtcgccgaag
     6181 ggttccgcga aatattatct gatatcctca aataaatatt tctcccgcag caagtatcat
     6241 tcctgtgccg cctatttgta ttttgagaaa aaaatcatct tttttagagt ccctgttgtt
     6301 ttcataacgg ggcacttttt tgatgtggct caatatcctt gagggccttc ccattgattc
     6361 accctggatg aaaaccgagt cctgtccgtc ttttacaaga tcatgaagga aaaggtagta
     6421 agtcagagct ccgtttgccg tcccggtggc cgcttcttcg tttatatcat aaaggggagc
     6481 aaaattcctg cagtgtgctg tgatatcatc ttctgccgta tcaagggtga aagcatgtac
     6541 tcccgtcact tcatattcct ctgaaaatct ggaaagagcg tcaaaatcag gatcaatatc
     6601 aaaaagtgta tttctgtccg aaaccggaag aaggatatcc ggaagacccg tagaaattat
     6661 catcggaagg agttttttct tatctccggg gctttggtga ataaaaactt cgcccacagg
     6721 aatacccata atatcaaaaa gtgtttcagt ctctgttttc ccgctgatgg tctttatgtg
     6781 ctcgggaatc gccatttcca tcataataaa atctttttgt gctgatattt caagatctcc
     6841 cgcaagagta tgattcatat acttctgttc ttttgatagt atgccttctt tgagcatcac
     6901 atagaaggcg ccgatagttg cgtgtccgca aagatccact tcggcagcag gcgtaaaata
     6961 tcttgtgtgg aattcctttt cattaagtct tttgacaaat gccgtctcgg agtatcttag
     7021 ttccgctgcg gttttttgca tcaatgatgc agaaggaaaa tcatctcctt caggtattat
     7081 aacaactccc gcagggttgc ccccgaaggc ggcggaagaa aaagcatcaa ctatgtaaaa
     7141 cttcatataa atatacctcc aacagaataa actataaacg caactatcca tgcaacggta
     7201 gtctgaaaaa gaaccaccag gatcgcatgc caccatccgt tcatttcttt tttgatcgtg
     7261 gcaagagttg caacacaggg catgtacaac agacagaata ccataaaaac aaaagcagtc
     7321 aagggtgtga atatctctat caacatggca gaaagggcgg ctccgtctgt ggctcccgta
     7381 aggactgcca tggtgcttac aacagcttcc tttgcggaaa ggccggttat aagagcagta
     7441 gaggcacgcc agtccgcgaa tcccagaggt tcaaacagtg gcactgcaac tttccctatg
     7501 gcagccagaa tgctgtcatc cggatctgat accatattca tgcccgcatc aaaactctgt
     7561 aaaaaccaaa taactatggt tgctatgaat actatagtaa aggctttttt tatgaattct
     7621 tttacgtttt cccacattct cagccacacg cttttcattg cgggaagcct gtagggaggt
     7681 agcacgagca caaaaggaac aggatctccc ttgtaaatag tatgcttgaa tataagagca
     7741 catagcacgg aaacaatgat acccataaga tatatggcgg ccattataaa gactctgtta
     7801 tcttcaaaga aagcggaaat gaacattgca tacagcggaa gtttggcact gcatgacatg
     7861 aaaggtatga gggcgatggt aagatgtctg tcccttttgc tgggcaggga tcttgtggcc
     7921 attattgccg gaacggagca gccgaagcct atgagcattg gtactattga ctgacccgaa
     7981 agaccgactt tgcgaagaag cttgtccatt acaaaagcca ctctgggcat atatccgctg
     8041 tcttccagga tggaaaggaa aaagaaaaga actgctatcc tgggtaaaaa agaaagaaca
     8101 ctgccgactc ccgcacatac tccgttaata ataagagagt gcagagcggg agcgactccg
     8161 ctgacggaaa ggccgttgtc tatcagagat actgtcttgt ctattcccat acccagccag
     8221 tcagaaagcc attgaccgat ggaatcaaaa gtaaaataga atataagcag catgatgccg
     8281 aaaaaaatcg gtatggcgaa tattctatgg gtcagtattt tgtctataat gagggaacga
     8341 tcctgctctc ttgtggtcga agccgttctt gttattacct ttgccgccag gtcctgtata
     8401 aaggcatatc tcatgtccgc catggcagct tcccgatccg tattcaaatg atgttccata
     8461 tcgctgatta tatcatctat tatttttaat tcgggatctt tgagatcaag ctgtttaagt
     8521 accggctcat cgccctccac tatttttgtg cagcagaacc taaggggaag gtcggccttc
     8581 tcggcatcat ctttaataag gtgtgctatt gagtgtatgg ctgtgtgcac atgccctgaa
     8641 caaaaatcta ttttttcggg aacggttttg tcttttgctg cttctatggc atagtgaata
     8701 aggtcgtgta taccccggtt tttgctggct gaaatgggaa taacgggcac tgtaagctct
     8761 ttttccagtt tttgaagatc aaaatcaata tcattgtttc ttacctcgtc catcatgtta
     8821 agggcaacaa ccattggtat gtccagttcc ataagctgaa gagtaagata aagacttcgt
     8881 tcaaagcctg tggcatccac aatgttgacg atcacatccg gtttgccgtt tatcagtatg
     8941 tctctggcgg tgatgtcatt tagcgtataa gcagaaagtg aataggtgcc cggcagttct
     9001 acaactgtta catttttgtg agcacgcaca ggcccttcct ttgccgtatg gcagcactca
     9061 aagaatcggc ccctctgttt tatgcccccg gtcattctgt tgaaaagtgt tgtggcgccg
     9121 cagtttgggt tacctattat ggcaacgctg acagtatcac tttttttatc gtgatgccgg
     9181 cgtctacgcc ggttttcatt cccttttctg cttttattca tctgatgttt tgtcctccgg
     9241 tttagaatag ggacaaatgt tgcagtttgt gttttctgaa cagtcttctg cagcgacttc
     9301 gatctcagaa gcttcggctt ttcgcagaga caactcaaaa ccccgaagac agatctcaac
     9361 aggatctccc agaggtgcaa ctttccttat cataactatg gtacctggtg tcagtcccag
     9421 gtcaaggagt cttctgcgca aagcgccttt gccttttact gcggttatcc ttgcccgggc
     9481 accgggtttc agcatgtcca gtgtcatatg atacctcatg atactttgtt tatgaacgct
     9541 aattgctcat atcggttagc caaagctaac tcaaaaaaat tataatccaa aacaaaacac
     9601 atgtcaattg aaacttggtt gagtatgata taataatacc cataaaaaca cagataaaat
     9661 cacggagaag gaataatgaa aaaaaagtat atcataaaag ggatgggttg cgccgcatgt
     9721 gctgcaacaa tagaaaagag tgtgaatgcc atgccttccg taagagaagc aagggtcaac
     9781 cttgtttccg attcaatgat aattgatttt gatgaaaagc agaccgggtc ttccgaaatc
     9841 attaaaacag tcagggaaag cggatatgat gcagaagaga caaaaagcac ctatgaatgc
     9901 aaaaaaaatg aagaaaaaac agcagtcagg gaaataaaag agctcaaata ccggttcctg
     9961 atatcactga tatttctgat ccctctgatg gttttgtcca tgggagcaat gataaatctg
    10021 ccggtcccgg aagtctctta ctggattctt ggcgttttag ctgtgcctgt ggcggccatc
    10081 aattacaaat attacacaag agggttcaag ctgcttttta aagcctcgcc caacatggac
    10141 tctctcattg ctataggctc tttggccgca ttgatactgg gatatttcga atcagcggtc
    10201 atgatcctga ctctggtgac cctgggtaag tggctggaag cacgatccaa gggcaggacc
    10261 ggtgatgccg taaaaaaact gatggccctt gctccggaag aagcaagaat aattacaaaa
    10321 gacgggcagg aaaaaatcat accggcagag gatcttttgc cgggagatat cattgcagtg
    10381 aaatcaggag aaagtatccc tgcagacggg acagtaacag aaggcacatg caccctggat
    10441 gaatcgaccc ttacggggga gagcatgccc gttgataaag ggcccggaga tgaagttttt
    10501 acagcgacca taaacaaggc cgggtacttg agaatgaagg cagacaaggt gggggaagaa
    10561 actgcatttt caggaataat aagacttgta gaggaagcag ccaattcaaa agctcccata
    10621 gcaaggcagg ctgataaagt gtctgctttt tttgttcctg ccgttatatt gattgctgtg
    10681 ataacggctg tctgctggat attagtcggg gcagaggctg atcaggtcgg ttccatgagc
    10741 attgcggtac tggtgatatc atgtccctgt gctttgggtc ttgctacgcc gttagccata
    10801 atgacaggaa cgggaagggg ggcagaaaag ggcatactct ttaggactgc agaggctctg
    10861 gaaacagttt caaagacccg ggtggcggtg atagacaaga ccggaacact tacaaaagga
    10921 aaacccgaaa taagtgaaat agtcacaata aaaggaacag aagaagatgt tatagcatat
    10981 acggcttccc ttgaaaaact ttcggaacac ccccttggct tacccataat agaaagagct
    11041 gagaaaaaag gactggatat aaaaaaagtc aataaatttc ttgtaatgcc cgggagaggg
    11101 atagaaggca taatagatgg aaagagggtt ttgtcgggca acaaagagtt catgcatgaa
    11161 aacgatatat tcacagaaga aggcaggacc gcaaaattgt atgagacagg aaggactctg
    11221 atatacacag gcatagacgg tgatcttgcc gggatcatcg cattaaggga cgagccgaag
    11281 gaaagcagcc gggaagccgt tcttgaacta aaagaaatgg gaatagaaac ggtcatgctt
    11341 acgggagaca atcaaatgac agcagaggtc attgcaaaag agacaggcat agacaaagtg
    11401 gtcgcacagg ttatgccgga agaaaaaaag aatgttatag atgatataaa aaaagaagga
    11461 aagtctgttt tgatgatagg agacggtata aatgatgcgc cggctctaaa aactgccgac
    11521 acgggaatag ccataggagc gggaacggat atcgcaatag aagcagctga tatcgttctt
    11581 atgcatgaag atccaaaaga cggggcttct gccgtgaagc taagcaaggc agtagtaaga
    11641 aacataaaac agaatctttt ttgggcattt ttctataact ctttgggaat ccctctggca
    11701 gcaggagtgt tttatccgtt tacgggatgg actctcacca gttggaccgc atcctctcgc
    11761 tcgggttg
//
LOCUS       DAONHK010000026        11700 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_4309_length_11700_cov_4.470846, whole genome shotgun sequence.
ACCESSION   DAONHK010000026 DAONHK010000000
VERSION     DAONHK010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 11700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11700
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4309_length_11700_cov_4.470846"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1262)
                     /gene="rpoN"
                     /locus_tag="PLM92_01825"
     CDS             complement(<1..1262)
                     /gene="rpoN"
                     /locus_tag="PLM92_01825"
                     /inference="COORDINATES: protein motif:HMM:TIGR02395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HPR23910.1"
                     /translation="MRLGYDLTIEQSQKLVMTPELIQAIQILQFNTQELDSYVQDQLL
                     SNPVLETSQAEEGSTENERKSFDEQTDLLREYIRERSYDETGYKQPQYANDDKDYSYE
                     QYISADITLPEHLMFQLQFASGNKTCRKIGRYIIESLDENGYMTLEDDEIAAAVETSI
                     KNVKKVINLIQTFEPMGVGARDLGQCLLIQLKSKGLYNEKYKSIIIEHIEDLAGNRLG
                     QIAKDLGISVKEVQEMSDVIKSLEPKPGRQFAGQTETRYIVPDVFVEKSEDGYSVAIN
                     DTSIPKLMVSSYYEKVLRESGNDQKLNEYLSERFNSAVWLIKSIEQRKQTIINVVRSI
                     VKFQKEFFDNGDKYLKTLTLKQVADDVGIHESTVSRSINGKYMQSPRGVFELKYFFSG
                     GVSGDRGEGISSNSIKTHIKEIIEKEDI"
     gene            complement(1308..1778)
                     /gene="trmL"
                     /locus_tag="PLM92_01830"
     CDS             complement(1308..1778)
                     /gene="trmL"
                     /locus_tag="PLM92_01830"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013920366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HPR23911.1"
                     /translation="MKFDIVLVEPEIPPNTGNIARSCAATGSVLHLVRPLGFSLDEKS
                     LKRAGLDYWPFVKLVTHENFGSFIAAYGKRRMHIVTTKGNKNYTEVSYQDGDMLIFGK
                     ETEGLPAGFIEENKEKTIRIPMRPETALRSLNLANSVSIVLFEALRQTGFSGLK"
     gene            1903..2913
                     /locus_tag="PLM92_01835"
     CDS             1903..2913
                     /locus_tag="PLM92_01835"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HPR23912.1"
                     /translation="MHTVKIGLLGLGNIGTGSYKTLEMNKDIILASTGIDLTITKILE
                     KDTQRKRDIKVSQDQFVKDPSEIFEDPEIEIVIELLGGIEPASSFMMEAMKNGKSVVT
                     ANKAAVAANFDKLNKTARENDVLFRFEASVGGGIPILNAITTVLRANQFQEILGILNG
                     TTNYILTQMTEYDLAYDGVLKTAQEKGFAEADPTADVEGEDCANKLSILMALAFGNYV
                     PPSDIPTKGITEITKEDIEAAKAKGCKIKLISQAKKENDKIIYSVKPMEIPFDHPLAG
                     VNNEFNAVFVKGNAVDELMFYGKGAGPLPTGSAIMGDVLEIARISAEKINNNKTEEKE
                     WK"
     gene            2904..3458
                     /locus_tag="PLM92_01840"
     CDS             2904..3458
                     /locus_tag="PLM92_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011098489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HPR23913.1"
                     /translation="MEMTSLYKEWKTLIENQTETSFDEFWENYSSTEERIYSKILDKA
                     LKEKTGNEESFENTGHVELQGKFSELAKDFEADPVIFEGFLDGINESLQNGLDIEKIT
                     PSSGISMDIDLEKLYFNMLKAGAKYLYDLPQWTSIFSAEKKSEIQKAYKRSKTLIKEK
                     KPGRNEPCPCGSGKKYKHCCGKGL"
     gene            complement(3461..4003)
                     /locus_tag="PLM92_01845"
     CDS             complement(3461..4003)
                     /locus_tag="PLM92_01845"
                     /inference="COORDINATES: protein motif:HMM:NF015999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HPR23914.1"
                     /translation="MRYKRYVQEGRALIAEEQQKVYDILKELDIEYRVCRHAPLVGAE
                     DRHVPGYHQPGLNVKNLVLKDKKTQEIYMVVLKDDARLDFKKYKEIAGWSKKISFADE
                     EDLKKYLGVSRGSCSVFSLINDREKRVRVVISEYIAKAPENEKISFHPNDNTATVTIS
                     VKDMYRFLAWTGNDVIHEGR"
     gene            complement(3964..4596)
                     /gene="nth"
                     /locus_tag="PLM92_01850"
     CDS             complement(3964..4596)
                     /gene="nth"
                     /locus_tag="PLM92_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009644605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HPR23915.1"
                     /translation="MTFSKQTINKILDRLKEEYPDAGCELLHEDVYQLLVKVTLSAQT
                     TDKSVNSISEKLFFEYPDAKAMAAADLQKLEDILRSVGMYKTKSKNIIAQAKILTEEF
                     DGIVPEDYESLVALPGVGRKTANVVLSVGFGHQRIAVDTHVFRVANRIGLTEEKDVLK
                     TELALMKSLPKDRWSESHHSLIFHGRRCCFARNPRCLECVIKDMCRRAGL"
     gene            4867..6462
                     /locus_tag="PLM92_01855"
     CDS             4867..6462
                     /locus_tag="PLM92_01855"
                     /inference="COORDINATES: protein motif:HMM:NF014218.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS1 family nucleobase:cation symporter-1"
                     /protein_id="HPR23916.1"
                     /translation="MATYTQVEKNGLFELTDAARAELVSSKYYNDDLAPTSVSQRTWT
                     TYNITMLWVGMSICIPSLSLASALIGMGVSPWLSVINVALGNLIVLIPMQLNSHVGTK
                     YGIPFPLFSRLTFGTIGAQIPALLRAVVACGWCSVQAYVGGGAVAALISIVAPKFKEA
                     DWTTNLPSWVGMQTANVGQVIGFFIFMAFILWVAYNGMENIKWVQNIGGPILIIVMIA
                     LLAWSANLAGDAGYSFGDVMHQTNDPSVIAASGGFAYIYMAGLTGNIAFWATMALNIP
                     DFSRYAKSQKVQFAGQLIGMPLPMFFCAFIGAFFAQATKLAMGAALFDPTAVFYYVDN
                     KFVVFISALGVVAATITTCVAANVVAPANGFSNMSPSKISYKKGVVITCLLSCFVLQP
                     WWIYGTGAAYIFGWLNNYGTILAPIAAIFIADYFVCKKMRVDVADLFKGSDGRYWYYS
                     GWNLAAIIAWVAAWIIPILGNTVFIYDPSTGVGPSFIQWMAANGYVVSFAIGFVIYWI
                     LMKGMAKNSFVTEEEHEAFTERI"
     gene            6615..8021
                     /gene="hydA"
                     /locus_tag="PLM92_01860"
     CDS             6615..8021
                     /gene="hydA"
                     /locus_tag="PLM92_01860"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013240213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HPR23917.1"
                     /translation="MDLLIKGGKVVGAKTTFKADVAVKNDKIVAIGNGLKAEKDTEVV
                     DARGKLVLPGAIDGHTHLAMPFGGTISTDDYYAGTRSAACGGTTTVFDFILQGQGEPM
                     LDSIKRRDALAAPDAAVDYSFHIGVTDVTTDKMLDSMEDAVKFGIPSFKVFMVYDFGV
                     DDGSFYKVLQKAKKIGALVGVHAENRDVNNVLVKQYLAEGKVEPWWHYMSKNEDVEGE
                     ADVRAINLAKMAGTSLYIVHLADKQGMDAVTAARAEGYPIFAETCPQYLNFTKEVYKS
                     KRAQDYVCSPPMKGKASQDAVWAGIKNGNIATLATDHCPFTRAEKDWGIKQKNGKPGN
                     FTTIPNGCAGIENMYPYILSEANKGRISFNRAVEISASNPAKLFGLDHLKGAIEVGLD
                     ADIVIYDPKKNLTVNNKKNMHGDLDHTIWEGANLKGYPVATYSRGKLVFDGKKFVGEK
                     GYGKLIKCKPIRHKGPIL"
     gene            complement(8177..8710)
                     /locus_tag="PLM92_01865"
     CDS             complement(8177..8710)
                     /locus_tag="PLM92_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPR23918.1"
                     /translation="MNSSKNTFKTAFGGICLAFTVVAVFMASIIPGLELTFYAVSSVF
                     TAAVIIESGIKSGFMLYGASCVLCFILIPDKAALIPFVFFFGIYGVIKFLAEKARNPF
                     VQIIIKTLFFMVIFGSGFFFFKSLFFGNISLPDYSLWVLIPGSLLIFILYDYIYTLMI
                     KLYRQRIKRDQKGIKLS"
     gene            complement(8749..9321)
                     /locus_tag="PLM92_01870"
     CDS             complement(8749..9321)
                     /locus_tag="PLM92_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HPR23919.1"
                     /translation="MGNPYEILGVSRNASETEIKEAYKKMVKKYHPDKYQDNPLGDLA
                     EEKLQEINQAYDQIMNERQGRSNDPYGQPNRPGPEFMEIRRDIDAGRLKQAQQKLDLI
                     QIKSAEWIFLDGMISYRKGWYDDAAGKIQQAVNMDPNNMEYQQALSRLMNAGQGYRNN
                     AYGRGYMSNQDAFCTACQCYLCADCCCDCI"
     gene            complement(9305..10237)
                     /locus_tag="PLM92_01875"
     CDS             complement(9305..10237)
                     /locus_tag="PLM92_01875"
                     /inference="COORDINATES: protein motif:HMM:NF039826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5685 family protein"
                     /protein_id="HPR23920.1"
                     /translation="MDCIIYGKRAIFVWGLILLGYVTCEKPEMKVRDFEIYNGYYCGI
                     CKSIGHRYGQIPRMTLSYDAAFLAVLLAGLEDEKDKVKREHCIIHHIKKKTIVSNGAV
                     DYAGDVMLILAWHKAMDDMRDEGKFSAKASLLLMKKIYNKIKGRYPGLCQKIQEQLKE
                     LAVIEKEKCSNLDMAAESFAIIMEEIFNGYVEKNEREQILLKRLGYHMGKWIYLMDAY
                     DDLEKDIENGSYNPLVYRYSKGEEEKAKDFAERIKEQCERNLLIYCSEMEKTIDLLDI
                     KKNKGIIENIVYMGLLRRTEKALGKEEAENGESV"
     gene            10327..>11700
                     /locus_tag="PLM92_01880"
     CDS             10327..>11700
                     /locus_tag="PLM92_01880"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005838454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HPR23921.1"
                     /translation="MSSTLAYKILKNHLISGELKAGEEITIAIDQTLTQDSTGTMVYL
                     QLEAMDIKKVKTELSVAYIDHNTLQTGFENADDHRFIKSVAKRHGIIFSKPGNGICHQ
                     LHLENYGKPGKTLLGSDSHTPTGGGLGMIAIGAGGLDVAVAMAKGTYSLTVPEVMGIK
                     LKGSLKPWVSAKDVILHILKELTVKGGVGKIIEYTGEGVSALSVTDRATITNMGAELG
                     ATTSIFPSDENTRQYLESQGREEDHIFLCADEDAVYDHTLEVDLSLLTPLAAKPHSPD
                     NVDTVFDIGEIKVDQVAIGSCTNSSYTDLMKVAAILKGSKVHPDVSLVISPGSSRILN
                     KMAENGALADLISSGARIIENACGPCIGMGQSPRSGAVSLRTFNRNFKGRSGTADADV
                     YLVSPETAAFSAIKGVLTDATTYGMILPSIEPAFFEPNYSFIVSADGGDTENTPLEMG
                     PNIKPFPR"
BASE COUNT         3309 a         2641 c         2369 g         3381 t
ORIGIN      
        1 tttatatctt ctttctctat gatttctttt atatgggtct ttatgctgtt tgaagatatt
       61 ccttctcccc ggtctccgga aacgcctccg gaaaaaaaat atttcaactc aaaaactcct
      121 cttggactct gcatgtattt gccgtttatt gaacggctca cagtggattc gtgtatgccc
      181 acatcatccg ccacctgctt gagtgtgagg gtctttaagt atttatctcc gttgtcaaaa
      241 aactcttttt ggaattttac aatagatctt acaacattta ttatggtctg tttccgctgt
      301 tctatgcttt tgataagcca tacggcagaa ttgaatctct ccgaaagata ttcattgagt
      361 ttctgatcat ttcccgactc tcttagaact ttttcgtaat atgaacttac cataagcttt
      421 ggtatgctgg tgtcatttat ggcaacagaa tatccgtctt ctgatttttc aacaaatacg
      481 tcaggtacta tgtacctggt ctctgtctgg cctgcgaact gtctcccggg ctttggctcc
      541 agggatttga tcacatcact catttcctgg acttccttaa cagatatacc cagatccttt
      601 gctatctgcc caagccggtt tcccgccaga tcttcgatgt gctctattat tatgctttta
      661 tatttttcgt tataaagtcc tttgcttttc agctgtatca aaaggcactg acccaggtct
      721 cttgccccca ctcccatggg ctcaaaagtc tgtataaggt ttattacttt ttttacgttc
      781 tttatgcttg tttctactgc tgctgcgatc tcatcgtctt cgagggtcat gtatccgttc
      841 tcatcaagag actctattat atatcgtccg atttttctgc aggtcttgtt accggatgca
      901 aattgcagtt ggaacataag atgttcggga agggttatat ccgcagatat gtactgttcg
      961 taggagtagt ctttatcgtc gtttgcatac tgaggctgct tatatccggt ctcatcatag
     1021 cttctttctc ttatatattc tctaagcaga tcagtctgtt catcaaaaga cttgcgctca
     1081 ttttctgtgc tgccttcttc cgcctgtgat gtctcgagca ccggattgct cagaagctga
     1141 tcctggacat atgaatccag ttcctgagtg ttgaattgca aaatctgaat cgcttgtatc
     1201 aactcaggag tcattaccag tttctgcgac tgttcaattg tcaggtcgta accaagtctc
     1261 atttcttttc tttcaacact ccaaatatat tatagcatac cgcctcttta tttcaacccg
     1321 gaaaagcctg tctgacgcag tgcctcgaac aaaacaatgc ttacagaatt ggccagattc
     1381 aaggatctca aggctgtctc gggccgcatt gggatcctta ttgttttttc tttgttttct
     1441 tcaataaagc cggctggcag tccttctgtt tctttcccga atataagcat atccccatct
     1501 tggtagctga cttctgtata atttttgttt ccttttgtgg taacgatgtg cattctcctc
     1561 ttcccatatg cagcgataaa ggagccaaag ttttcatgcg tcacaagttt tacaaagggc
     1621 cagtaatcaa ggcctgccct tttcaaactt ttttcatcaa gagaaaaccc caggggcctg
     1681 acaagatgaa gcacactgcc tgttgccgca caggatctgg cgatattacc cgtattaggg
     1741 ggtatttccg gttcaacaag aactatgtca aatttcattt tcagcctcct ttgtatatct
     1801 taataaaaat tacttctttt ataaagacaa atcaaagaaa atagtatata attttttaaa
     1861 ttcaccatat cggaatacac tatttaatcg ggaggtaaat caatgcacac agtaaaaatc
     1921 ggtcttctgg gccttggcaa cattggtaca ggctcatata aaactcttga aatgaacaaa
     1981 gatatcattc ttgcttctac gggcatagac cttactataa caaagatcct cgaaaaggat
     2041 actcaaagaa aaagagatat caaggtttct caggatcagt tcgtaaagga tccttccgag
     2101 atattcgaag acccggaaat cgaaatagtc atagaactgc tgggaggcat cgaacccgct
     2161 tcgtctttta tgatggaagc catgaagaac ggaaaaagcg tggtaactgc caacaaggct
     2221 gccgtggcag ccaattttga caagcttaac aaaaccgccc gggaaaatga tgttttgttc
     2281 agatttgaag ccagtgtagg aggaggcatc cctatcctta atgccataac aaccgtacta
     2341 agagccaacc aattccagga aatacttggg atacttaacg gaactacaaa ctatatactt
     2401 acacaaatga ccgaatacga tcttgcatat gacggcgtac tcaaaaccgc ccaggaaaag
     2461 ggttttgcag aagccgatcc tacagctgat gtggaagggg aagattgtgc caacaagctt
     2521 tccatactta tggctctggc tttcggtaat tatgtgccgc cttcagatat cccgacaaaa
     2581 ggtattactg agatcacaaa agaagacatc gaagcagcca aagcaaaagg atgtaagatc
     2641 aaactgattt ctcaggcaaa aaaagaaaac gacaaaatta tttacagtgt gaagcccatg
     2701 gaaataccct tcgatcatcc cctggccggt gtaaacaatg aatttaatgc ggttttcgta
     2761 aaaggaaatg cggtggatga acttatgttt tacgggaaag gtgcaggtcc gctgcccaca
     2821 ggcagtgcca taatgggaga tgtccttgaa atagccagga tatcagcaga aaaaatcaat
     2881 aataacaaaa cagaagaaaa ggaatggaaa tgacatcact atacaaagaa tggaaaaccc
     2941 ttatagaaaa tcaaaccgaa acatcctttg atgagttctg ggaaaactac agcagcacag
     3001 aagagcgtat atattcgaaa atactggata aagctcttaa agaaaaaacc ggaaatgaag
     3061 agtcctttga aaacaccggt catgtggagc tccagggcaa attcagtgaa cttgcaaagg
     3121 attttgaagc agatcctgta atattcgaag gttttctgga cggtataaac gaaagcctgc
     3181 aaaacggcct agatatagaa aaaatcacac cgtcctccgg tatttccatg gatatcgatc
     3241 ttgagaagct ttacttcaat atgcttaaag ccggtgcaaa gtatctttat gatcttcccc
     3301 agtggaccag cattttttct gcagaaaaga aaagtgaaat acagaaagct tataagagat
     3361 caaaaaccct tataaaagaa aaaaagcccg gaaggaacga gccctgcccc tgcggaagcg
     3421 gcaaaaaata caagcactgc tgcggtaaag gtttataaga tcagcgccct tcatggatca
     3481 catcgttccc tgtccatgca aggaatctgt acatatcttt tacacttatg gtgaccgtgg
     3541 cggtgttatc attgggatga aaacttattt tttcattttc tggggctttg gcaatatatt
     3601 cagagatcac tacccgcact cttttttctc tgtcgtttat tagactgaaa actgaacatg
     3661 aaccccggga gacaccaaga tattttttaa gatcttcttc atctgcgaag gagatctttt
     3721 tgctccatcc ggctatctct ttatactttt tgaagtccag ccttgcatca tccttaagaa
     3781 ctaccatgta tatctcttgg gtctttttgt cttttaagac cagatttttc acattgagtc
     3841 ccggctgatg ataaccgggc acatgacgat cttcggctcc caccagcgga gcatgccggc
     3901 aaactctata ttctatatca agctctttta atatatcata tactttttgc tgctcttccg
     3961 ctatcaaagc cctgccctcc tgcacatatc ttttataacg cattccaagc accgtggatt
     4021 cctcgcaaag cagcatcttc tgccatgaaa aatcaggctg tgatgacttt ctgaccagcg
     4081 atccttgggc aggctcttca tcaacgccag ttcggttttc agtacatcct tttcttctgt
     4141 aagccctatc ctgttggcca cccgaaatac atgggtatcc accgcgatcc tctgatgccc
     4201 gaagccgacg gaaagaacca catttgccgt ttttctgccc acaccgggca gtgccaccag
     4261 actttcgtaa tcttcaggaa cgataccgtc aaattcttct gtaagtattt ttgcctgagc
     4321 tattatattc tttgactttg ttttatacat cccaacagac cttaatatgt cttcgagttt
     4381 ttgcagatcc gccgctgcca tggcctttgc atccggatat tcgaaaaaca gcttttctga
     4441 tatggagttc acgctcttat ctgtagtctg ggccgaaagg gttactttca caagaagttg
     4501 atacacatct tcatgcagaa gttcacatcc cgcatcggga tactcttctt tcagtctgtc
     4561 aagtattttg ttgattgttt gctttgaaaa tgtcactaat ttgctccttc tgagaggtca
     4621 tatttcacag gatctacgtt tttcatacaa ggagtatagc atttatataa tcaaaaaaac
     4681 aaattttttc gcttgttttc caaaatcaat gatctttatt tggaatatat attatttttt
     4741 tgaaagaaaa gataacttga atgtttaaaa agtttctgaa aaaccaaata aatgggttac
     4801 ttttttgcta ttatgtagta taatcgtaat ataataatct acaatatttt atacggaggt
     4861 gtttaaatgg caacttacac acaagtagaa aaaaacggcc tgtttgaatt aacagatgcc
     4921 gcgagagctg aacttgtctc gtcaaagtac tataacgatg acctggcacc gacttcagtt
     4981 tctcagagga cctggacgac ctacaacatc acgatgctgt gggtgggaat gtcgatatgt
     5041 attccctctc tgtcactggc ctcagcactg ataggcatgg gagtgtcccc gtggctttcg
     5101 gttatcaacg ttgcactggg caacttgatc gtacttatcc ctatgcagct gaactcacat
     5161 gttggtacca aatacggtat cccgttcccg ctgttttcaa gacttacttt cggaacaata
     5221 ggtgctcaga tacctgctct cctaagggcc gtcgttgcct gtggatggtg ttccgtacag
     5281 gcctatgtag gcggcggagc cgttgctgct cttatcagta tcgtagctcc gaaattcaag
     5341 gaggccgact ggactaccaa ccttccttca tgggtaggaa tgcagacagc aaacgttgga
     5401 caggttatcg gattctttat cttcatggct ttcattctct gggtagcata caacggaatg
     5461 gaaaacatca aatgggtaca gaatatcggt ggaccgatcc tcatcattgt aatgatagct
     5521 ctgctggcat ggtcagccaa tctggccggt gatgcgggct acagcttcgg agatgtaatg
     5581 catcagacaa atgacccgtc agttatcgca gcatcaggag gatttgcata catatacatg
     5641 gcaggtctta cgggcaatat agcattctgg gctacaatgg cacttaatat ccctgacttc
     5701 tcacgttatg caaaatcaca gaaagtgcag ttcgcaggac agcttatagg aatgcctctg
     5761 ccaatgttct tctgtgcatt catcggagct ttcttcgcac aggccaccaa actggccatg
     5821 ggcgcagcat tgtttgatcc tacggcagta ttctactatg tagacaacaa attcgtcgta
     5881 ttcatttctg ctttaggcgt agtagcagct acaataacca cttgcgtagc agctaacgtt
     5941 gtagctccgg caaacggatt ctcaaacatg tctcccagca agatctctta caaaaaaggc
     6001 gttgttatca catgcttact ttcttgtttc gtactgcagc cctggtggat atacggtaca
     6061 ggcgcagcct acatcttcgg atggctgaac aactatggta ccatacttgc tcctatagct
     6121 gctatattca tcgctgacta cttcgtatgt aagaagatga gggtagacgt tgcggatctg
     6181 ttcaagggaa gcgacggcag atactggtac tacagcggct ggaaccttgc agcaataatc
     6241 gcatgggtag ctgcatggat aattccgatc cttggcaaca ctgtatttat ctatgatcct
     6301 tcaacaggcg taggtccgag cttcatccag tggatggcag ccaacgggta tgtggtatcg
     6361 ttcgcaattg gatttgtgat ttactggata ctcatgaagg gtatggccaa gaattctttc
     6421 gtaacggaag aagaacacga agcatttacg gaaagaattt aatctgaagg taaaacttca
     6481 taaaagcaag ttaaaatgca agagcctttg ggatgactca gaggctcttg ctttaccccc
     6541 taaaaaaagg gagtaacaat tatattttgt ttttgcgcaa aaacaaaaag acagtttata
     6601 aaggaggaca aaatatggat ttactaatca aaggcggcaa agtcgtaggt gctaaaacca
     6661 cattcaaggc ggacgttgcc gtaaaaaacg ataagatcgt tgccattggt aatggtctta
     6721 aagccgaaaa agacaccgaa gtagttgatg ccagaggcaa gcttgttctt cccggagcca
     6781 tcgacggaca cacacatctg gccatgcctt tcggcggaac catatccaca gatgactatt
     6841 atgcaggcac cagatccgcc gcctgcggag gaacaacgac agtatttgat ttcatcctgc
     6901 aaggccaggg tgaacccatg ctggattcca tcaagagaag agacgctctt gctgctccgg
     6961 atgcggctgt tgactattcc ttccatatcg gggttacaga tgtcaccacc gacaaaatgc
     7021 tggattccat ggaagatgcc gtaaaattcg gtattccttc attcaaggta ttcatggtat
     7081 atgatttcgg tgttgatgac ggttcattct acaaagttct gcagaaagcc aaaaaaatag
     7141 gagctcttgt gggagttcat gcagagaaca gggacgtgaa caatgtactt gtcaaacaat
     7201 acctggcaga aggcaaagtt gagccctggt ggcattatat gtccaagaac gaagatgttg
     7261 aaggcgaagc tgatgtaaga gccatcaatc tggcgaaaat ggcaggcacc tccctttata
     7321 tcgtacatct ggcagacaaa cagggtatgg atgctgttac cgccgccaga gcagaaggct
     7381 atcccatttt tgcagaaaca tgcccccagt atctcaactt caccaaagaa gtatataaaa
     7441 gcaaaagagc tcaggactat gtatgctcgc ctccaatgaa gggtaaggct tcccaggatg
     7501 ccgtgtgggc cggaataaag aacggtaaca tagccactct ggccacagac cactgcccgt
     7561 tcacaagggc ggaaaaagac tggggcatca aacagaaaaa cggcaaaccc ggcaacttca
     7621 caactatacc aaacggttgc gccggcatcg aaaacatgta tccctatata ctttccgaag
     7681 ccaacaaagg caggatcagc ttcaacagag ccgttgagat cagtgcatca aatccggcaa
     7741 aactgtttgg gctggatcat ctgaagggtg ctatcgaagt gggtcttgat gcggatatcg
     7801 ttatttatga tcccaagaag aatctgacag ttaacaacaa aaagaatatg cacggcgatc
     7861 tggatcatac catttgggaa ggcgcaaatc tcaagggtta tcctgttgcc acatacagca
     7921 gaggcaaact tgtattcgac ggcaaaaaat tcgtaggcga aaaagggtac ggcaaactta
     7981 tcaaatgtaa gcccatcagg cacaaaggtc caatactcta aaaacagcat tatgtaacga
     8041 cccgaataga aataaaagag aagcctgtgg cttctctttt atttctattt atctcttttc
     8101 tttgtatttt tatattttat aatgcatatc agtcgctgtc attctgcttc tttgttccgt
     8161 tcctgccatg gttaccttat gacagcttta tacccttctg gtcccttttt attctctgcc
     8221 tgtacaactt tatcataagt gtgtatatgt aatcatataa tatgaaaatc aaaaggctcc
     8281 ccggtatcag tacccataat gaataatccg gcaaagaaat atttccgaaa aagagagatt
     8341 tgaaaaagaa aaatccgctt ccgaaaataa ccataaaaaa aagagtttta ataattatct
     8401 ggacaaaagg gtttcttgct ttttcagcaa gaaacttgat cacaccatat ataccaaaaa
     8461 agaatacaaa ggggatcagt gcagctttgt caggtataag gataaagcaa agaacacaag
     8521 atgccccgta gagcatgaac cctgacttta tgcccgactc gataataaca gctgcggtaa
     8581 aaacagaact aactgcataa aaagtgagtt ccagcccggg aatgatggat gccataaaca
     8641 cagcaacaac tgtaaaggcc agacatatac cgccaaaggc tgtcttaaag gtattcttgc
     8701 tgctgttcat atgattctcc gttttcataa aactacagag tacttgtttt atatgcagtc
     8761 gcagcagcag tccgcacaca gatagcactg gcaagccgta cagaatgcat cctgatttga
     8821 catataacct ctgccgtatg cgttgtttct gtatccctgc ccagcattca tgagtctgct
     8881 cagtgcctgt tgatactcca tattgttggg atccatatta acagcctgct gtatcttgcc
     8941 ggccgcatca tcataccatc cttttctgta tgatatcatc ccgtcaagga atatccattc
     9001 cgcacttttt atctgtataa ggtcgagttt ctgctgtgct tgttttagtc ttcccgcatc
     9061 aatatctctt cttatttcca tgaactccgg cccggggcgg ttaggctgcc cgtatgggtc
     9121 gttgctcctt ccctgtcttt cgttcatgat ctgatcatat gcctgattta tttcctgaag
     9181 tttttcctcg gcaagatccc ctaagggatt gtcctgatat ttgtcgggat gatatttctt
     9241 caccattttt ttgtaagctt cttttatctc tgtctctgac gcattccggc ttactcctaa
     9301 gatctcatac ggattcccca ttctctgctt cttcctttcc aagggccttc tcggtccttc
     9361 tcaaaagtcc catataaact atgttttcta ttataccttt attcttttta atatcaagca
     9421 aatctattgt tttttccatt tccgaacaat atatcagcag atttctttca cattgctcct
     9481 ttatcctttc ggcaaagtcc tttgcttttt cttcttctcc cttggaatat ctgtaaacaa
     9541 gaggattata ggatccgttt tcaatatctt tttccagatc atcataggca tccataagat
     9601 atatccattt gcccatatgg tagcccagtc ttttgagcag aatctgttcc ctttcgtttt
     9661 tctcgacgta gccattgaat atttcttcca ttataatggc aaaagattcc gccgccatat
     9721 cgagattgga acatttttct ttttcgatga cagccagttc ttttaactgc tcttggattt
     9781 tttggcacag acccggatat ctgcctttta ttttattata aatttttttc attagaagaa
     9841 ggcttgcttt tgcggaaaac tttccttcat ctctcatatc atccattgcc ttgtgccatg
     9901 ccagaatgag cataacatct cctgcataat ccactgcccc gtttgagact attgtttttt
     9961 tcttaatatg atgtatgatg cagtgctctc tttttacttt atccttttca tcctcaagac
    10021 cggcaagaag cacggccaaa aaagccgcat cataactgag tgtcattctt gggatctgtc
    10081 cgtaacgatg tcctatggat ttgcatatac cacaataata tccgttgtat atctcgaagt
    10141 cccgcacctt catttccggt ttttcacaag ttacatatcc taacaaaatc aacccccata
    10201 caaaaatggc tcttttcccg taaattatac agtccatgaa catttatgta aagcagaact
    10261 tgaccgttta cacagtgtat cgtataattg tatacaaata tatgagtcat ccataaggag
    10321 gtaaaaatga gcagcacttt ggcgtacaaa atacttaaaa atcatcttat cagcggcgaa
    10381 cttaaggccg gcgaagaaat aaccatcgcg atcgatcaga cccttactca ggattctaca
    10441 ggcaccatgg tttatcttca gcttgaagct atggatatca aaaaggttaa aacagaactt
    10501 tcagtagcct atatagatca caatactctt cagacgggtt ttgaaaatgc cgacgatcac
    10561 cggttcataa aaagcgtggc aaaaagacac ggaataattt tttcaaagcc aggaaacggc
    10621 atatgccatc aactgcacct ggaaaactac ggaaaacccg gaaagaccct tctgggtagc
    10681 gacagtcaca caccgaccgg aggcggtctg ggtatgatag ccataggtgc cggcggtctt
    10741 gatgttgccg ttgccatggc caaaggcaca tacagcctta ctgttcccga agtcatgggc
    10801 ataaaactca agggctctct gaaaccctgg gtctccgcca aagatgtgat tctccatatc
    10861 ctaaaggaac ttaccgtgaa aggcggtgtg ggcaaaatca tagaatatac aggagaaggt
    10921 gtttctgccc tttccgttac tgacagagct accatcacaa atatgggtgc agaacttgga
    10981 gcaaccactt ctattttccc cagcgatgaa aacaccagac aatatcttga gtcccagggg
    11041 agagaagaag accatatctt cctttgtgcc gatgaagacg cagtttacga tcacactctg
    11101 gaggttgatc tttcactgct tacacctctg gcggcaaagc cccacagtcc cgacaatgtg
    11161 gatactgtct ttgacatcgg tgaaataaaa gtggatcagg tggccatagg cagctgcacg
    11221 aattcttcat ataccgatct aatgaaagta gcagcgatac ttaagggcag caaagtgcat
    11281 ccggatgtga gtcttgtaat atctcccggt tcatccagga tacttaataa gatggcagaa
    11341 aacggagctc tggccgatct tatttcttcg ggggcaagga taatcgaaaa tgcatgcggt
    11401 ccgtgtatag gcatggggca gtccccaaga tccggagccg tctctcttcg cacatttaat
    11461 cgtaatttca aagggagaag cggaacagcc gatgcagatg tatatcttgt cagccccgag
    11521 acagcggctt tttcggctat aaaaggtgtg ctcacagatg caactaccta cggcatgata
    11581 ctgccttcca tcgaaccggc tttttttgaa cccaattaca gtttcattgt ttctgccgat
    11641 ggcggcgaca ccgaaaacac tcctttggaa atgggtccca acataaaacc tttcccgaga
//
LOCUS       DAONHK010000027        11525 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_4384_length_11525_cov_4.888230, whole genome shotgun sequence.
ACCESSION   DAONHK010000027 DAONHK010000000
VERSION     DAONHK010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 11525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11525
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4384_length_11525_cov_4.888230"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="PLM92_01885"
     CDS             <1..294
                     /locus_tag="PLM92_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPR23922.1"
                     /translation="VGMMADKDVDGIISILADSAKDFIATEPDNSRKLKAEILCGKLT
                     EKGALCHVVPDVKEAFLEAQKRKDGFGLIVFAGSLYMIGDIRKNFWRASDNDK"
     gene            284..1225
                     /locus_tag="PLM92_01890"
     CDS             284..1225
                     /locus_tag="PLM92_01890"
                     /inference="COORDINATES: protein motif:HMM:NF009605.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HPR23923.1"
                     /translation="MINEKRKKVLLFYNPHSGDGMFKNNLDFIIEKVQTKGMQLIPIR
                     AARDNMIETVFKSIKQEDYRQIIVAGGDGTINICVNSMIQNNVDLPLSIFPAGTANDF
                     AYYFGLPHDIEDMLRIAMGENYTYADVGRCNQKYFVNVAAMGTLVDVSQKTDPNLKNT
                     IGVISYYLKGLSEVPNLKPLPIKLITPEKTYEEKMYFMVVMNGKSAGGFKRISPDSDI
                     NDGKLDVVLFKEMPIVELLPLLVSISQGRHPKHKKVLAFQTEKLRLESDVEVPTDVDG
                     ERGEKFPLEFEVVHNKLKIFTQSENIEEKDAGKKRRL"
     gene            1200..1646
                     /locus_tag="PLM92_01895"
     CDS             1200..1646
                     /locus_tag="PLM92_01895"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPR23924.1"
                     /translation="MLEKNADYKSLIEFFIENGLEYDNEKAAECIVCSWQIKEGPQDS
                     ITAGALLVKSKGEFILDGIAVSESYRKNSKGSLLLGEVIKKVRQLGGKRIYLTAKVPD
                     FFRKAGFETISFQDGPDYFDCLSCPQYGKKCQPEAMVLEVKNENNS"
     gene            1630..2649
                     /gene="plsX"
                     /locus_tag="PLM92_01900"
     CDS             1630..2649
                     /gene="plsX"
                     /locus_tag="PLM92_01900"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013780672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HPR23925.1"
                     /translation="MRIILDGMGGDNAPAAIVEGAVRASEEIDDEIYIIGIEDAINEE
                     LKKYKYDENRIHVFNADEVISNQEAPVKAVRTKKGSSLVKGIGMVKEEEGDLFISAGS
                     TGALMAAGLFTLGRIPGIDRPAIATIYPMMGGIPSLLVDAGANSECKPDNLLEFGIMG
                     SIYMEKVLDRKNPRVGLVNIGEEENKGSTLTKAAHELLDRSHINFIGNVEARDVPKGV
                     CDVIVCDGFVGNVILKLTEGLAWNILKLLKKKFTEGAKAKLGAVFLAGKMKELKAEFD
                     YSEYGGAPILGVKGPLIKMHGSSGAKAVKNTIIKGIPYAEEKVVDTIQNAVLDIEEII
                     ISEQD"
     gene            2636..3331
                     /gene="rnc"
                     /locus_tag="PLM92_01905"
     CDS             2636..3331
                     /gene="rnc"
                     /locus_tag="PLM92_01905"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003869194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HPR23926.1"
                     /translation="MNRTEFQQLIGYYFKDMELFEKALTHSSFFRGRGMGPGKDNERL
                     EFLGDALLDAVISEELYNRMPSGEEGKLSKTRALVVCEASLAQIGNSLLIGDILRLGK
                     GEDISGGRHRDSIIADAVEAVIGAVFLDGGFDASKNVVLPLFEKTLKLATEGKLKSDY
                     KSEFQEIVQSSGTAEINYVLNREEGPDHDKTFYVNLFVDGQEAGKGTGKSKKEAEQNA
                     AKEAIARRKQNVF"
     gene            3321..6869
                     /gene="smc"
                     /locus_tag="PLM92_01910"
     CDS             3321..6869
                     /gene="smc"
                     /locus_tag="PLM92_01910"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HPR23927.1"
                     /translation="MYFKRIEMQGFKSFAEPVTIEFDKGITCVVGPNGSGKSNISDAI
                     RWVLGEQSPKMLRGGKMEDIIFAGTSSRKSRGMAEVTLVIDNSTGILPIDYNEVAITR
                     RMYRSGESEYAINNNQCRMRDIRELLMDTGIGVDGYSLIGQGQIADIISNKSDSIRDI
                     FEETAGIVSYRTKKAEAERKLSSTTSNMERVNDIIREIEGRIGGLKEDSEKAALYLEL
                     RDRYKELEINITLKNIENIEKKNREFKNDAGSLQNELEEVRNSVDRIEKELGTDREKN
                     EELEKQCNETRARLLDIIEEINSLVNRSEVNGERLASIEKNNKRLLMEIAILEEKLSK
                     EKNDSKEQFESKERIDRELSSLSDVLEEKIADHARIYEALNEKTEETERLKERLFSLH
                     GDISSKKAEMKSLQNLKDTLKNRRVEVLQQKESGESDSINAVSALEDAKAKRDSLSSE
                     MKRIRENTEKTRKSYNDILQNERSLAVKLEELKIEISRLSSRKKTIEEMESNYEGYNN
                     AVKFIMRSETGGIHGVVAELINVPEGYEVAMETALGAALQNIICEDDRAAREAIDQLK
                     QNKAGRLTFLPMNSIRPGRKENRDDLIKMPGFMGYAVDRIGFDQKYKNVMEYLLGRVV
                     IADNMENAILMSKKTGGGARYVTLEGEIINSGGAITGGRYKNKTANLLERKAEITKME
                     TRLAEMSSEKQKLEEEQAGMRRALEEKLVVIKENEEIFREKEREFFAGENEVKVMQTA
                     LDDFENSSEKWQRELANIDREQALSDETVKNLDIGSRELEEEIKKTEREIENLSAVTE
                     KEKQRADVASEEITGARIALGAAESEKTKIDAIVNRINETIKEAEKEKETKVGELEYL
                     KKERETILSGVSSNEGLVHEKEEEKERLSKELEEMNSEKSSTNKKIGLASDEREELLK
                     KITTLQNNKYEAEIRIARQETQLENYKEKLWEEFEISYIQAIEFRKQDFVMAPAVKEN
                     REIKTKIKELGEVNVGAIKEYKTVKERYDFLNGQKNDIEISMTELRKIIEDMDKIIKA
                     RFKESFDRIAENFELVFKEFFGGGTANIRLDNEEDPLNAVIEITAQPPGKQLRHINLL
                     SGGEKTMTAIALMFAVLKTKPTPCCILDEVEAALDDSNIDIFANYLKNFDDIQFTLIT
                     HQKATMEHADVMYGVTMPERGISKIYSLSLGEEPLM"
     gene            6912..7811
                     /gene="ftsY"
                     /locus_tag="PLM92_01915"
     CDS             6912..7811
                     /gene="ftsY"
                     /locus_tag="PLM92_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009644632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HPR23928.1"
                     /translation="MTLEKQQKSFFGKMSRRLSDAIMARPSIDDGLLEDIEEILITSD
                     IGMETTEKIIYALREDVRKDRLDTPEKVRERLKEIMISLVDKEQRHQLSEEYPMVILM
                     IGINGGGKTTTIGKLTHKLNKQGKTVLLAAADTFRAAAGEQLSIWARKAGVNVIIHKE
                     GADPSAVIFDAIMSAKSKGTDILICDTAGRLQNKKNLMAELAKMNKVIDREFPEASRE
                     TLLVLDATTGKNAVSQVGEFSSITDITGIVITKLDGTAKGGIALTIADEFDMPIKFVG
                     TGESIEDLQKFVAEEFIGGIFDE"
     gene            7804..9129
                     /gene="der"
                     /locus_tag="PLM92_01920"
     CDS             7804..9129
                     /gene="der"
                     /locus_tag="PLM92_01920"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014314066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HPR23929.1"
                     /translation="MSKPLVAVVGRPNVGKSTFFNKVVGRRVSIVEDTPGVTRDRIYA
                     EAEWRNITFGIIDTGGIDAGSEEVIPASMREQAEIAMDTSDVILFMTDGREGLTADDR
                     DVAQILRMTGKKVVLVVNKIDTAKMPADFYDFYELGLGEPIAISAENMLGIGDLLDIV
                     IENFPEGSDQQEEDLIRIAVTGKPNVGKSSLINEILGENRVIVSPVAGTTRDSIDTPF
                     EKDGDKYLLIDTAGIRRHSKVNENIEKYSVIRAVTSIERCDVCLVMIDAQDGVTEQDK
                     KIAGLAHEAGKGIVIVVNKWDLIKKETGTMKEFQKQISREMAFLSYAPSVFISVKEKQ
                     RIEGVIAFAKSVAESRAMRVPTGQLNSLIADAVMMKQPPSDKGKRLKIYYAAQVGIKP
                     PLFSFQVNSRALMHFSYSRYLENRIRDVYGFSGTSIKFVFREKGEKNNV"
     gene            9122..9754
                     /gene="plsY"
                     /locus_tag="PLM92_01925"
     CDS             9122..9754
                     /gene="plsY"
                     /locus_tag="PLM92_01925"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HPR23930.1"
                     /translation="MYEFFTGYETDDIRIKVITVITMILAYFIGNISPATIIGRIMGI
                     DIKKEGSGNAGTTNVLRVLGAKAAVLTLAVDVLKGVAAVLIGYYAAGIPAAMLCAPSV
                     TAGHIWPVLLRFKGGKGVATIFGTLTALEPLMGLTALALVAAGAFISRRASVGSIVGA
                     VTLPFVAWFFMPKLIVIACLLGALVLFSHRSNIVRLVKGEEPRINFGGKS"
     gene            9751..10746
                     /locus_tag="PLM92_01930"
     CDS             9751..10746
                     /locus_tag="PLM92_01930"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HPR23931.1"
                     /translation="MKKIGVIGAGSWGTALAITLGSKGHEVKIWDLDAVHLEECKKNR
                     ENVRYLPGIKFNDNLQMADTAEEAIGGADIVLFSAPAQHFRSAFKGAMPYITEDMVVV
                     NVAKGIEQKTLCRMSQVAEELMPGVKYVVLSGPSHAEEVGKLLPTTVAVASRDRKLAE
                     YIQDIFITERFRVYTNDDVVGVELGGSLKNIIALGAGISDGVGFGDNAKAALMTRGMA
                     EISRLGIALGAEKETFLGLSGVGDLIVTCTSMHSRNRRCGIMIGEGMKPEEATKKVGM
                     VVEGMFTTEAAYELAKKAEVEMPITEGIYRIIKGEISAKEAAEELMGRNRRHEKE"
     gene            10733..>11525
                     /locus_tag="PLM92_01935"
     CDS             10733..>11525
                     /locus_tag="PLM92_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006000505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPR23932.1"
                     /translation="MKKNRKFHIQTLGCQMNERDSETIAGILSEMGYSVAADRNEADV
                     AVINTCSVREHADKRFFGTLGQLKKIKEARPDFVVCVCGCMMQQKHITDTIKEKYPWV
                     DIIFGTHNIHELPVLLQKAEKEKESRKRSEKNGIMGKGKGLISVWENGEGIVEDLPSL
                     RLYRHKAFVNIIYGCNNFCTYCIVPYTRGRERSRKPEDILNEVKKLAADGVKEITLLG
                     QNVNSYKGLSGPAGVSYGETNEGQKHTDFADLIYMLQETEGIQRIR"
BASE COUNT         4098 a         1961 c         2950 g         2516 t
ORIGIN      
        1 gttggcatga tggcggataa ggatgtggac ggtattatat ccatacttgc agattcggca
       61 aaagatttta ttgccacaga gcctgataac agccgcaaac tgaaagcaga gatcctttgc
      121 gggaaactca ctgaaaaagg cgctttatgc catgtggtac cggatgtaaa agaggccttt
      181 cttgaggcac aaaaaagaaa agacggattc ggactgatcg tttttgcggg atcattgtat
      241 atgatcggcg atataagaaa aaacttttgg agggcatcag acaatgataa atgaaaaaag
      301 gaaaaaagtg ctgctttttt ataatcctca ctccggtgac ggaatgttca aaaataacct
      361 ggattttata attgaaaaag tgcagacaaa gggtatgcag ctgattccca taagagccgc
      421 cagagacaat atgatagaaa ctgtctttaa aagcataaaa caggaagact acaggcagat
      481 aatagtagct ggcggtgacg gaacgatcaa tatatgtgta aattcaatga tacaaaacaa
      541 tgttgatctt ccgctgtcga ttttccctgc gggaactgca aacgattttg catattactt
      601 cggactgccg catgatatag aagatatgct gagaatcgcc atgggcgaaa actatacata
      661 tgctgatgtg ggaaggtgca accaaaaata ttttgttaat gtggcggcca tggggactct
      721 ggtggatgtt agccagaaaa cagatcccaa cctgaaaaac accatcgggg tgatctcata
      781 ttatctgaaa ggtctttcgg aggtgccaaa cctgaaaccg cttcccataa aactcataac
      841 acccgaaaag acatacgaag aaaaaatgta tttcatggtc gttatgaatg gaaaatctgc
      901 aggcggtttc aaaagaatat ctcctgattc tgatataaat gacggaaagc tggatgtagt
      961 tcttttcaaa gagatgccca tagttgagct tttaccgctg ctggtaagca tatcccaggg
     1021 gaggcatccg aaacataaaa aagttttggc ttttcagacg gagaaactga ggctggaatc
     1081 cgatgtggaa gtgcctactg atgtggacgg tgagagaggg gagaagttcc ctctggagtt
     1141 tgaagtagta cataacaaac ttaaaatatt tacgcagtcc gagaatatag aggagaagga
     1201 tgctggaaaa aaacgcagac tataaaagcc tgatagagtt ttttatagaa aatggcctgg
     1261 aatatgataa cgaaaaagcc gcagagtgta tcgtttgttc atggcagata aaggaaggtc
     1321 cgcaggacag cataactgcc ggtgctctgc ttgttaagag caagggtgag tttattctgg
     1381 atggaatagc cgtttcggaa agctacagaa aaaacagcaa aggctcgctc cttttgggag
     1441 aagtaataaa aaaagtcagg cagctgggag gaaaaagaat ataccttaca gcaaaagttc
     1501 cggatttttt caggaaggca ggatttgaaa cgatatcctt ccaagatggt ccggattatt
     1561 tcgattgttt gtcatgcccg cagtacggca aaaaatgcca acctgaggca atggttctgg
     1621 aggtgaaaaa tgagaataat tcttgatgga atgggaggag acaatgctcc tgcggcaatt
     1681 gtggaaggag cagtcagggc ctcggaagaa atagatgatg aaatatatat aataggtatc
     1741 gaagatgcca ttaatgaaga actgaaaaaa tataaatacg acgaaaacag aatacatgtc
     1801 ttcaatgccg atgaagtaat aagcaaccag gaggcaccgg tcaaagcggt aagaacaaaa
     1861 aaaggatcat ccctggtaaa agggatagga atggttaagg aggaagaggg agatcttttt
     1921 atttctgccg gatcaacagg tgctcttatg gcggcgggcc tttttaccct gggcaggata
     1981 ccgggaatag acagacctgc cattgccacc atatatccaa tgatgggggg catcccctct
     2041 cttctggttg atgccggcgc aaattccgag tgcaaacctg ataatctgct ggaattcggc
     2101 ataatgggaa gtatatatat ggaaaaggtg cttgacagaa aaaatcccag ggtcggactt
     2161 gttaatattg gagaagaaga gaataaaggc agtacactta caaaggctgc tcatgaactt
     2221 cttgacagga gtcatataaa ttttataggt aatgtggaag ccagagatgt gccaaagggc
     2281 gtttgtgatg ttatcgtatg cgacggcttt gtgggcaatg ttatactaaa actgacagag
     2341 ggactggcat ggaacatact gaagcttctt aagaagaaat tcacagaagg agcaaaggca
     2401 aaactgggtg cggtcttcct tgcaggcaag atgaaagaac tgaaagctga atttgattat
     2461 tccgaatacg gaggagctcc gatactggga gtaaaagggc cactcataaa aatgcacggt
     2521 tcttcaggag caaaggctgt caagaacaca ataataaaag gcataccata tgcggaagaa
     2581 aaggtagtag atactattca aaatgcggtt ttggatattg aggagataat tataagtgaa
     2641 caggactgaa tttcagcagc tgatagggta ttacttcaag gatatggaac tgtttgaaaa
     2701 agcattgact cacagctctt ttttccgcgg gagaggcatg ggacccggca aggacaatga
     2761 gcgccttgaa tttctgggag atgctcttct tgatgcggtt atcagtgaag aactttataa
     2821 caggatgccc tcgggcgaag aaggaaagct ttcaaaaaca agggctcttg tggtctgtga
     2881 ggcttctctg gcacaaatcg gcaattctct tcttattggg gatatacttc gtcttggaaa
     2941 aggagaagat ataagcggag gaagacatag agattctatt atagccgatg cagtagaagc
     3001 ggtaatagga gcagtattcc ttgacggtgg gtttgatgct tccaagaatg tcgtgctgcc
     3061 tctttttgaa aaaactctga aactggccac ggaagggaaa ctgaaaagtg actataagtc
     3121 tgaatttcag gaaatagtgc aaagcagcgg aaccgccgaa ataaattatg ttcttaatcg
     3181 ggaggaaggg ccggatcatg acaaaacttt ttatgtaaat ctttttgtgg atggacagga
     3241 agccggcaaa ggtaccggca aaagcaagaa agaggccgag cagaatgctg caaaagaggc
     3301 catagcgagg agaaaacaga atgtatttta agcgtatcga aatgcagggc ttcaagtcat
     3361 ttgcagaacc tgtgactata gagtttgata aaggtataac atgtgttgtc ggacctaacg
     3421 gaagcggcaa aagcaacata agtgatgcca taagatgggt acttggagaa cagagcccca
     3481 aaatgttaag gggcggtaaa atggaggata ttatatttgc gggaacatcc agcagaaaat
     3541 caaggggcat ggcagaagtt actcttgtaa tagataattc gacggggata ttgcccatag
     3601 attataatga ggtggctata acaagaagga tgtatcgctc gggagaaagc gaatacgcaa
     3661 taaacaataa tcagtgcagg atgagggaca taagggagct ccttatggat accggtatag
     3721 gtgtggatgg atattcactt attgggcagg ggcagatagc tgatataata agcaacaagt
     3781 cggacagtat ccgtgacatt tttgaagaga ctgccgggat agtaagctac agaacaaaaa
     3841 aagctgaagc cgaaagaaag ctttcatcca caacgtcaaa catggaaaga gtcaatgaca
     3901 taataagaga gatagaagga aggataggcg gactgaagga ggacagcgag aaggccgcat
     3961 tatatcttga actcagagac agatataaag aacttgaaat aaatataaca cttaagaata
     4021 tagaaaatat cgaaaagaaa aaccgtgaat tcaagaatga tgccggatcg ctccaaaatg
     4081 agcttgaaga agtcagaaac tccgtagacc ggatagaaaa agagctggga acagacagag
     4141 aaaaaaacga agagctggaa aagcaatgta atgaaacaag agccaggcta cttgatataa
     4201 tagaagaaat caattcactg gtgaacagaa gcgaagtaaa cggcgaaagg cttgcttcca
     4261 tagagaagaa caacaaaagg cttcttatgg aaatagccat attagaagaa aaactgagca
     4321 aagagaaaaa tgattcgaag gaacagttcg aaagcaaaga gcgtatagac agggaacttt
     4381 cttctctttc cgatgtgctg gaagaaaaga tagcagatca tgccaggata tatgaagcat
     4441 taaatgaaaa aacagaggaa acagagcggc tgaaggaaag acttttcagt cttcacgggg
     4501 atataagctc aaaaaaagcg gaaatgaaga gcctccagaa tttgaaagac acattgaaaa
     4561 accgccgggt agaggtgcta caacaaaaag aaagcggaga gagcgacagc ataaatgcag
     4621 tgtctgccct ggaagacgct aaggccaaaa gagattcatt aagctctgaa atgaaaagga
     4681 taagagaaaa taccgaaaaa acaagaaaga gctataatga tatcctccaa aatgaaagat
     4741 ctcttgcagt aaaacttgaa gaactgaaaa tagaaataag caggctttcc tccagaaaaa
     4801 agacaataga agaaatggaa agcaattatg aaggttacaa caatgcagta aagttcataa
     4861 tgagatcaga aacaggcggg atacacggtg ttgttgcaga gcttataaat gtacctgaag
     4921 gatacgaagt ggcaatggag acagctcttg gagcagcact gcagaacata atatgtgagg
     4981 atgacagagc tgcaagggaa gctattgacc agctgaaaca gaataaggcg ggcagactga
     5041 catttcttcc catgaactcc ataagacccg gaagaaaaga aaaccgggat gaccttataa
     5101 aaatgcccgg gttcatgggg tatgcagtgg accggatcgg ctttgatcag aaatataaaa
     5161 atgtgatgga gtacctgctg ggcagagtcg taatagcaga taatatggaa aatgcgatac
     5221 ttatgtcgaa gaagacgggt ggcggcgcaa ggtatgtcac tcttgaagga gaaataataa
     5281 attccggagg ggcaataaca ggaggcagat acaaaaataa aacagcaaat ctacttgaaa
     5341 gaaaagcaga aatcaccaaa atggaaacac gccttgcgga aatgagttcc gagaaacaaa
     5401 aacttgaaga ggagcaggcg ggcatgcgca gagcccttga agaaaaactt gtggtgataa
     5461 aagagaatga agagattttc agagaaaaag aaagagagtt ttttgccggt gaaaatgaag
     5521 tgaaagtaat gcagacagcc cttgacgact ttgaaaacag tagcgaaaaa tggcaaagag
     5581 aacttgcaaa tatagataga gaacaggcac tttctgatga gacagtcaaa aacctggata
     5641 ttggatcaag agaactggaa gaagaaatca aaaaaacaga aagagaaatc gaaaatctgt
     5701 cggcagtaac agaaaaagaa aagcaaaggg cggatgttgc ctcagaagaa ataacggggg
     5761 caaggatcgc acttggtgcc gcagaaagtg aaaaaacaaa aatcgatgca attgtaaaca
     5821 gaataaatga gactattaaa gaggcagaga aagaaaagga gacaaaggtc ggggaactgg
     5881 aatatctgaa aaaagaaagg gaaaccattc tttcgggagt cagcagcaat gaaggacttg
     5941 ttcatgaaaa agaagaagaa aaagaacgtt taagcaaaga acttgaagaa atgaacagtg
     6001 aaaagagcag tacgaacaaa aagatcggcc tggcttccga cgaaagggaa gaactgctga
     6061 aaaaaataac gacactgcag aacaataagt atgaagccga gataaggata gcaagacagg
     6121 aaactcaact ggaaaactac aaagaaaaac tctgggagga attcgagata tcctacatac
     6181 aggccataga gttcaggaaa caggattttg taatggctcc cgcagtaaaa gagaacaggg
     6241 aaataaaaac aaagatcaaa gagcttggag aagtgaatgt cggagcaata aaggagtaca
     6301 aaacggtaaa agaaagatat gactttctga acggacaaaa aaatgacatt gagatatcca
     6361 tgactgagct ccgtaaaatc atagaggata tggacaaaat aataaaggca agattcaaag
     6421 aaagttttga ccggatagcc gaaaactttg aactggtttt caaagaattt tttggtgggg
     6481 gaacggcgaa tataaggctg gataacgaag aagatcccct gaatgctgta atagaaataa
     6541 cagcacagcc gccgggcaaa cagcttagac atataaatct tctttcggga ggagaaaaaa
     6601 caatgaccgc tatagcgctt atgtttgcgg tcttaaagac aaaacccact ccctgctgta
     6661 tacttgatga ggtggaagct gccctggatg acagcaacat agatatattt gcaaattatc
     6721 tcaagaattt tgatgacata cagttcaccc tcataactca tcagaaagcg accatggaac
     6781 atgcagatgt gatgtacgga gtaacgatgc ctgaaagggg catatcaaag atatattctc
     6841 tttcccttgg cgaagaacct cttatgtaaa actgccgtaa taacaagcgg cagtcaaaaa
     6901 aggaagaaaa aatgactctt gagaaacaac agaaaagttt tttcggcaaa atgtcaagaa
     6961 gactgtcaga tgccataatg gcaaggcctt caattgatga tggactgctg gaagatatag
     7021 aagagatcct tataacatcc gatataggca tggaaacgac ggaaaaaatc atatatgcct
     7081 taagggaaga tgtaagaaag gaccgacttg acacgccgga aaaagtaaga gagcgtttaa
     7141 aagaaataat gatctctttg gtggataaag aacagagaca ccagctttca gaagaatatc
     7201 ccatggtcat acttatgata ggcataaacg gaggcgggaa aactactacc ataggaaagc
     7261 tgacacacaa gcttaataaa caggggaaaa cagttctttt ggctgcggca gataccttca
     7321 gagccgctgc gggagagcag ctttccatat gggcccggaa ggcgggggtc aacgttataa
     7381 tacacaagga aggagcagac ccttctgctg ttatattcga tgccataatg tcggcaaaat
     7441 caaagggaac agatatcctt atctgtgata ccgccggcag gctccaaaac aaaaagaacc
     7501 tcatggcgga acttgcaaaa atgaacaaag taatagatag agaatttccc gaagcatcca
     7561 gagagaccct gctggtactt gatgctacca ccggcaaaaa cgctgtttct caggttggag
     7621 agtttagtag cataacagat ataacgggaa tagtaataac aaaacttgac ggaacggcaa
     7681 aaggcggaat agctttgaca atagcagatg aatttgatat gccgataaaa ttcgttggta
     7741 caggagaaag catagaagac cttcaaaagt ttgttgctga agagtttata gggggaatat
     7801 ttgatgagta agcccctggt tgccgtcgtc ggaaggccca atgtggggaa atccacattt
     7861 ttcaataaag tggtgggacg cagagtttcc atagtcgaag acacaccggg tgtcacaaga
     7921 gacaggatat acgccgaagc cgaatggaga aacataacat ttgggatcat agatacggga
     7981 ggtatcgatg cgggaagtga agaggtcatc cctgccagta tgagggagca ggcggagata
     8041 gcaatggata cttccgatgt catacttttt atgaccgacg gaagagaagg ccttactgct
     8101 gatgacagag atgtggctca gatcctcaga atgacaggca aaaaagtcgt tcttgtggtc
     8161 aacaagatag atactgccaa gatgcccgct gatttttatg acttttatga actgggactc
     8221 ggagagccga tagccatatc tgcagagaac atgcttggca taggagatct gcttgatata
     8281 gtgattgaga atttccccga aggatcagat cagcaggaag aagatctgat aaggatcgca
     8341 gtgaccggca aacccaacgt gggaaagtcc tcactgataa atgagatact gggagaaaac
     8401 agagttatag tatccccggt tgcgggaacc acaagagatt ccatcgatac tccatttgaa
     8461 aaagacggag acaagtatct cttgatagat acggcgggaa taagacgtca cagtaaagta
     8521 aacgagaata tagaaaaata cagtgtaatc agagcggtga catcaataga aagatgtgat
     8581 gtctgcctgg taatgataga tgcacaggac ggagtgactg aacaggacaa aaaaattgcc
     8641 ggacttgccc acgaagcggg aaaaggcatc gtgatcgtgg taaacaaatg ggacctgata
     8701 aaaaaagaaa caggcaccat gaaggaattt cagaaacaaa taagcagaga aatggctttc
     8761 ttgtcctatg caccttcagt atttatttcc gtcaaagaaa agcaacgcat agaaggggtc
     8821 atagcatttg ccaaaagtgt tgccgaaagc agggccatga gggttcccac gggacagctc
     8881 aacagtctga tcgcagatgc tgtgatgatg aagcagcctc cttctgacaa aggcaaaaga
     8941 cttaagatat actatgcagc ccaggtaggg ataaagcctc cgcttttttc attccaggta
     9001 aactccagag ctcttatgca tttttcttat tcgagatacc tggagaacag gataagagat
     9061 gtttacggat tcagcgggac ctccataaaa tttgttttta gagagaaggg tgaaaaaaat
     9121 aatgtatgaa ttttttaccg gatatgagac agatgatata agaatcaaag tgatcactgt
     9181 cattaccatg atattggcct attttatagg gaatatttct cctgcaacca ttataggaag
     9241 gattatgggg atagacataa aaaaagaagg cagcggcaat gcaggcacta ctaatgtatt
     9301 gagggtactt ggagcaaagg ctgcagtgct gacactggct gtcgatgttc ttaagggagt
     9361 ggctgcagtt ctgataggct actatgcagc ggggatcccg gcagccatgc tttgtgcccc
     9421 ttccgtgaca gcaggacata tatggccggt tctcctcagg ttcaaaggcg ggaagggcgt
     9481 tgcaactatt ttcggcacgc ttacggccct ggagccgtta atggggctta ctgctcttgc
     9541 ccttgtggca gcgggagcat tcatatcaag aagagcctcg gtgggatcaa tagtaggggc
     9601 ggtgacattg ccctttgtag catggttctt catgccgaaa ttaatagtta ttgcatgttt
     9661 acttggagcg ctggtactgt tttcacacag aagcaatata gtaagacttg taaaaggcga
     9721 agaaccaagg ataaattttg gagggaaatc atgaaaaaaa taggagttat cggtgcggga
     9781 agctggggca cggctctggc tataaccctg ggtagcaagg gtcatgaagt aaagatatgg
     9841 gatcttgatg ccgtacatct ggaagaatgc aaaaaaaaca gagagaatgt aagatatctt
     9901 ccgggaataa agtttaatga taatcttcaa atggcggata ctgcagaaga agcaataggc
     9961 ggtgccgata tagttttgtt ttctgcaccg gcacagcatt tcagaagtgc tttcaaaggt
    10021 gcaatgccat atatcactga agatatggtc gtagtaaatg ttgcaaaggg aattgaacaa
    10081 aaaactcttt gcaggatgtc tcaggtggcg gaagaactga tgccgggggt aaaatatgtg
    10141 gttttatccg gaccttctca tgcggaagaa gtgggtaaac ttttacccac cacagttgct
    10201 gtggcatcaa gagacagaaa gttggctgaa tacatacagg acatttttat aacagaaaga
    10261 ttcagagttt ataccaatga tgatgtggtg ggagttgagc tgggaggttc cctcaaaaac
    10321 atcatagctt taggtgcggg aatatccgac ggggtaggat tcggtgacaa tgccaaggct
    10381 gcactaatga caagaggtat ggcggagata tcaagactgg gcatagctct gggagctgaa
    10441 aaagaaactt tcctcggcct ttccggagtg ggcgacctta ttgttacctg cacaagtatg
    10501 cattcgagaa acagaagatg tggaataatg ataggagaag gaatgaaacc cgaagaggcg
    10561 acgaaaaaag tcggtatggt cgtggagggg atgttcacca cagaagccgc atatgaactg
    10621 gcaaaaaaag cagaagttga aatgcctata acagaaggga tatacagaat aataaaagga
    10681 gaaatcagtg cgaaagaagc tgcagaggag ctgatgggac gaaacagaag acatgaaaaa
    10741 gaataggaaa ttccacatac agaccttagg ctgtcagatg aatgaaagag attcagaaac
    10801 gatagccggg atattatcag aaatgggata ttccgtggcc gcagaccgaa atgaagctga
    10861 cgttgcagta ataaacacct gtagcgtcag agaacatgcg gacaaacggt ttttcggcac
    10921 attgggacag ctaaaaaaaa taaaagaggc tcgtcccgat tttgttgttt gcgtatgcgg
    10981 atgcatgatg cagcagaaac atataacaga taccataaaa gaaaagtacc catgggttga
    11041 tataatattc ggcacacata acatacatga actgcctgtt ctgcttcaaa aagccgaaaa
    11101 agaaaaagag agcagaaagc gttccgaaaa aaacgggatc atgggaaaag ggaaaggcct
    11161 gatatcagta tgggaaaacg gagagggcat agtggaagac ctgccgtctt tacgacttta
    11221 cagacacaag gcatttgtga acataatata cggatgcaat aatttctgca catactgtat
    11281 cgtgccttat acaagaggca gggagagaag cagaaaacct gaagatattt tgaatgaagt
    11341 aaaaaaactc gccgcagacg gagtcaaaga aatcactctt ctggggcaga acgtgaattc
    11401 atataaaggc ctatccggtc cggcaggtgt ttcatatggt gaaacaaatg aagggcaaaa
    11461 gcacacggat tttgccgatc tgatatatat gctgcaggaa acagagggca tacagcgtat
    11521 aagat
//
LOCUS       DAONHK010000028        11356 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_4464_length_11356_cov_4.517034, whole genome shotgun sequence.
ACCESSION   DAONHK010000028 DAONHK010000000
VERSION     DAONHK010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 11356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11356
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4464_length_11356_cov_4.517034"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /gene="leuD"
                     /locus_tag="PLM92_01940"
     CDS             <1..239
                     /gene="leuD"
                     /locus_tag="PLM92_01940"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016321124.1"
                     /note="catalyzes the isomerization between
                     2-isopropylmalate and 3-isopropylmalate in leucine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HPR23933.1"
                     /translation="FARIFYRNAINIGLPILECEKAGENICNGDEVAVDFDSGEITDL
                     TKNEKYTALPFPDFIRNIIDKGGLLKSIEKTKEK"
     gene            240..1382
                     /gene="leuB"
                     /locus_tag="PLM92_01945"
     CDS             240..1382
                     /gene="leuB"
                     /locus_tag="PLM92_01945"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HPR23934.1"
                     /translation="MNYNIAVIPGDGIGPEVIRETIKVLDRIADKFGHKFSYQHLLAG
                     GSAIDKTGKALPEDTISAVKSSDSILLGAVGGWKWDNLPGDQRPETALLALRKILGLY
                     ANIRPALLFEELASACPLKPEIIKGGLDLVVVRELTGGIYFGEKGTKETNMGHCAYDV
                     ELYSEKEVKRIAQTAFDMASKRNKKVTSVDKANVLESSRLWRRVVSETAKEYPQIELN
                     NMYVDNAAMQLVRDPKQFDVIVTSNIFGDILSDEASQITGSIGMLPSASLATGNFGMY
                     EPVHGSAPDLAGKDKANPMAAILSAAMMLRYSFGLLEEAEEISSAVRNVLKKGYRTPD
                     LMETALPEETADMTNKAWVKKEIGTKEAGDLISEEICPFILSVEKA"
     gene            complement(1357..2163)
                     /locus_tag="PLM92_01950"
     CDS             complement(1357..2163)
                     /locus_tag="PLM92_01950"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HPR23935.1"
                     /translation="MRDHFTTGEFAKLCRVKKQTLFHYDDIGILSPEIVARNGYRYYS
                     YKQLETFYMIATLKELDMPLKDIREYMDRRSPENFVTLLEAQAVKAEESIRKLQHMKN
                     YINKKLNITKEAIDIEKEKIHIEAQPKEHFIITEYSGPNEDEDIDKAITRHFNYCKEL
                     GIQSAYGIGGMIKTGNVCEKNGYRYAYFYTRTEKLSVPGFFTKPGGEYLTYYDPNGYS
                     KIEEVAAKLISHANENGLKAGKYFYEELALDGLSSKKLYDYTIKLSLQIK"
     gene            2249..3583
                     /locus_tag="PLM92_01955"
     CDS             2249..3583
                     /locus_tag="PLM92_01955"
                     /inference="COORDINATES: protein motif:HMM:NF013703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HPR23936.1"
                     /translation="MEHLFDNGVTVKRFFGFVSPSVIMLLVIGLYSVIDSIFIANCVN
                     THALAALNIVYPVFGFIWGVSVMLAAGSSALVAIKMGEGKSLEANRKFTLIYIVAAII
                     ALVMILPAFIFIDELLLFLGASDVLWDYCRTYGIIIIFAMPAGFIGVLFEFFIRVDGK
                     PGFTLALYIVSGTVHVVLDVVFLVYLDWGIEGAAWATFAGMYSTVIMGACYFIFRDTK
                     LKFVKTKMDWAYIGHSMSNGSSEMVSESSVGIMVFFFNYIMMDLAGETGVAALTIVLE
                     ILYFMVSVYLGYVTGVAPLISYYYGAGRYEKVNCFLKYSKNFILFSSILISAVCLFFA
                     PMLSGVFVPAGSQVYDMATTGLRFMAIGFLFTGINIFASGFFTAYGNGKISALISLCR
                     GLVLVIVLGFLLSYLFGINGVWLALVMTDIVTVILSFAMFKRYREKYHYSFR"
     gene            3653..4207
                     /locus_tag="PLM92_01960"
     CDS             3653..4207
                     /locus_tag="PLM92_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008718274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HPR23937.1"
                     /translation="MSLFELLLTATGLAMDAFAVSVCKGVTLKEKTTRYAITAGLWFG
                     IFQGLMPAAGYFAGSAFSVLVDSFDHWIAFILLSFIGIKMIVESRKKDCPSNPSFKSA
                     IMFPLAVATSIDALAAGVSFGFLKVDILQASLIIAAVTFVLSFLGVKIGHIFGCRFRS
                     GAELFGGIALIAIGTKILVQSFLC"
     gene            complement(4232..4633)
                     /locus_tag="PLM92_01965"
     CDS             complement(4232..4633)
                     /locus_tag="PLM92_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPR23938.1"
                     /translation="MKIIRKNLIYCGIISALGAGISVAGFYREDDLLKSIGIALVIVS
                     LLKIAQLFILTRNDERRRKYELSMTEERIKYIASKSSSFVFYITVFCEVIAALILLVF
                     GYVFAASVVCYVAGAQVVLYLVLYIIFSRKY"
     gene            complement(4630..4863)
                     /locus_tag="PLM92_01970"
     CDS             complement(4630..4863)
                     /locus_tag="PLM92_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004014548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPR23939.1"
                     /translation="MENNIAKLRKERKITQEELADAVSVTRQTIISIENGRYMASLPL
                     AFKIAEFFEKTIEDIFVPGDFNNEKTKNREDRK"
     gene            complement(4959..5294)
                     /locus_tag="PLM92_01975"
     CDS             complement(4959..5294)
                     /locus_tag="PLM92_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005562318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HPR23940.1"
                     /translation="MDMTTAEMLLMVAAVTIGVQIMRWVPFALFPEGREVPEAMTLLS
                     KVLPAAMMGMLVVYCFRNISPLEYPFGIPELISAAVVLALHFRIGNSILSIVAGTICY
                     MIMQQYIFV"
     gene            complement(5285..5971)
                     /locus_tag="PLM92_01980"
     CDS             complement(5285..5971)
                     /locus_tag="PLM92_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012201173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HPR23941.1"
                     /translation="MGEKKRAFKAAFPNTISIMAGSTSLGVVYGMLMETAGISAPWAI
                     AMSAVAFCGSMQFAAVPVLAASVFEPMQVLVLSLMINARHLFYGISMLEKYKDMGKLK
                     GFMVYTLCDESFSTESHCTVPQKVDRKWFYFFMSLMIYFYWVAGTAIGSLAGMLITFD
                     TTGMDFAMTALFIVLFTEYWKKKENRSAAVMGVLASLTALLVFDGVTFMIPAMLLMLM
                     AVFVRKKIWI"
     gene            6099..6959
                     /locus_tag="PLM92_01985"
     CDS             6099..6959
                     /locus_tag="PLM92_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016838880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HPR23942.1"
                     /translation="MDKYSVFLKIVETGSFTKAAQDLGYTQSAVSQIVRSLEKELFSS
                     LLLRDRNGATLTSEGRDLLPYIRSLSNSHRQLTEKANDLHGLKGGTVRIGTYSSMSCH
                     VLPPLMKSFGSKYPEIKFELLQGDYDATANWIKTGRVDFGLLPPKAFPHLEITVLKKD
                     RMMAVLPPGHHLSKRKTVSLSDLADESYIHLEEGSFSEPFQAFDELGLFPHIRFRVHD
                     DYTIMSMVESGLGVSVLSELVLNRTSYNIEIRETDPAVIRTIGIACKSRRALSAANKE
                     FINYIMKEIK"
     gene            7220..8251
                     /gene="aroF"
                     /locus_tag="PLM92_01990"
     CDS             7220..8251
                     /gene="aroF"
                     /locus_tag="PLM92_01990"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HPR23943.1"
                     /translation="MIIILKENPDKKQLENLTDWLKTMGLDIHYSQGTSTTIMGLIGD
                     TSVVDTDLINALDIVETVKRITEPYKNANRKFHPFDTVVEVPLSGGGTVKIGGGNFQI
                     IAGPCSVESEEQVCTIAMEVKEAGAGLCRGGAFKPRTSPYAFQGMREQGINLLLEAKK
                     RSGLPIVTEVMDISHLPFFENVDVIQVGARNMQNFELLKELGHTGKPVLLKRGLSSTL
                     RELLMSAEYVMAGGNSNVILCERGIRTFETATRNTLDLSAIPLLKEKTHLPVIVDPSH
                     ATGSAALVMPMAMAATAAGADGLMIEVHNDPEHALCDGAQSITPATFRDIVKSVKSIL
                     PLAYKGGKP"
     gene            8248..9087
                     /locus_tag="PLM92_01995"
     CDS             8248..9087
                     /locus_tag="PLM92_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014119578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HPR23944.1"
                     /translation="MIGVIGLGLIGGSLAKALNQNTDYSVYGYDIDRSVLSKAKLVGA
                     IEDELNENMLCECDIVIVALYPSDTVDYVRSNAENFKKGSIVIDCCGVKKPVCSILEP
                     LAQKHGFIFIGGHPMAGLEHSGFEYSKKALFDKASMVLVPPAGIGIKHLEKIKGLCSS
                     IGFSNIQISTADEHDQMIAFTSQLAHITSNAYIKSSAAIRHAGFSAGSYYDMTRVAKL
                     NEKMWTELFINNPEYLSCEIQELIDHLNEYKNAIDSKDKEKLTALLKDGREKKEKIDN
                     EFF"
     gene            9102..10172
                     /gene="aroB"
                     /locus_tag="PLM92_02000"
     CDS             9102..10172
                     /gene="aroB"
                     /locus_tag="PLM92_02000"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HPR23945.1"
                     /translation="MNNIIIKTGIPYQVIMEEDSLEKVGFFASDITEPCRICIISDDN
                     VHSLYTPLVESSLKNAGFDVYKIVFPAGEATKDLGSVKKILEYLADENFTRSDVLMAL
                     GGGVIGDLTGFAASVFLRGIKYINVPTSFLAAADSSVGGKTGVNLSAGKNLAGTFWQP
                     SLVIYDPKTVKTLSPASFLDGLAESIKCAFIADASLFGFIDLSDMNNETKFVERCVTD
                     SVKVKQFFVENDEYDKGRRQLLNFGHTAGHAIEKLSGFRESHGHAVAQGMVIAARASY
                     NKGWSREDCCSPLIEVLNKYGLSSSCSYSAAELAAAALKDKKRLGNEINLVIPLEIGE
                     CALMRLPSHELQNFFEAGLTGR"
     gene            10200..>11356
                     /locus_tag="PLM92_02005"
     CDS             10200..>11356
                     /locus_tag="PLM92_02005"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HPR23946.1"
                     /translation="MDIMIMPKPLKGKIKAVDSKSHVHRLLIAAALTKDPAKVVTVTT
                     SDDIEATRSCLAELENDHPVFQCKESGSTLRFMMPISMALKERAVFRCKGRLPERPVS
                     PLKEAMEAHGCSFSKRMDGWELDGRLGSGDYFIPGDVSSQYVTGLLFALPLLAGHSAI
                     RLTSPLQSESYVDMTIKTLEDSGIKIETRAPAKDGSCNTAAGEFPGGSYLVPGRQKYR
                     APLSVRPEGDWSNSAFWYAANKMSSRNNIVCTGLSSGSLQGDKKIGKFLSLLPGNIDV
                     SDTPDLVPPIAAAAALTSGTTLIKGASRLRLKESDRLRTVSQTLSDLGADIKELSEGL
                     EIKGKNILTGGTVNGSGDHRIVMMAAIAASVCQTPVIISGAQAVNKSYPSF"
BASE COUNT         3225 a         2439 c         2422 g         3270 t
ORIGIN      
        1 gctttgcccg cattttttac cgcaatgcca taaacatcgg ccttcctatc cttgaatgcg
       61 aaaaagcggg tgaaaatata tgcaacgggg atgaagtcgc tgtagatttt gattcgggtg
      121 aaataacgga tctgacaaaa aacgaaaaat acactgctct tccctttcct gatttcatca
      181 ggaacataat cgataagggc ggccttctta aaagtatcga aaagacaaag gagaaataaa
      241 tgaactataa catagctgtc atacccgggg acggcatagg ccccgaagtc ataagagaga
      301 ccatcaaagt ccttgatcgt atcgcagaca agttcggaca taaattttca tatcagcatc
      361 ttttagccgg gggaagtgcc atagacaaaa ccggcaaggc actgccggaa gacacaataa
      421 gtgcagttaa atcatccgat tcaatccttc tgggagcagt cggaggatgg aagtgggata
      481 atcttcccgg agatcaaaga cccgaaactg cccttttggc ccttcgcaaa atactgggac
      541 tctatgccaa tataagacct gctttgcttt ttgaagaact tgcttcagct tgtcccctta
      601 aacccgaaat aataaaaggc ggtcttgatc ttgtagtggt gagggagctt acaggaggca
      661 tatatttcgg cgaaaaggga acaaaagaaa cgaatatggg ccactgcgcc tatgacgtgg
      721 agctttattc cgaaaaagaa gtaaaacgca tagctcaaac tgcctttgat atggcatcaa
      781 agagaaacaa aaaggttacc agcgtggaca aggccaatgt tcttgaaagc tcaagactat
      841 ggagaagagt ggtttctgaa accgcaaaag aatatcccca aatagaatta aacaatatgt
      901 atgtagacaa tgccgccatg cagctggtac gggatcccaa acaattcgat gtcatagtta
      961 caagcaatat cttcggagat atactttccg atgaagccag ccagataaca ggatcaatag
     1021 gtatgctccc ttcggcaagt ctggcaacag gcaatttcgg catgtacgaa ccggtgcacg
     1081 gttctgctcc ggatctggca gggaaagata aggccaatcc aatggctgcc atactgtctg
     1141 ctgccatgat gctcagatac agtttcggtc ttttggaaga ggctgaagaa atcagctctg
     1201 cagtcagaaa tgtcctgaaa aaaggttatc gaactcccga tcttatggaa acggcgctac
     1261 cggaagaaac tgctgatatg accaataaag cttgggtaaa aaaagaaatc ggcacaaaag
     1321 aagccggaga tcttatatcg gaagagatat gcccttttat tttatctgta gagaaagctt
     1381 gatcgtgtaa tcatagagtt tcttgcttga caacccgtca agagccagct cttcataaaa
     1441 atatttgcct gccttaaggc cgttttcatt tgcatgggaa atcagctttg cggcaacctc
     1501 ttctattttg ctgtagccgt taggatcata gtatgtcaga tattcccctc cgggtttagt
     1561 gaaaaacccg ggaacggaaa gcttttctgt acgcgtatag aaataagcat atctgtatcc
     1621 gtttttttcg cagacatttc ctgtctttat catacccccg ataccgtatg cactttgtat
     1681 gcccagttcc ttgcagtaat tgaaatgtct tgttatggct ttgtctatat cttcatcttc
     1741 attggggcct gaatattcgg ttataataaa atgctctttc ggctgcgcct ctatatgtat
     1801 cttttctttt tctatgtcaa tggcttcttt ggtgatatta agttttttgt ttatatagtt
     1861 tttcatatgc tgcagcttcc tgatactttc ttcagccttt acggcctggg cttcaaggag
     1921 agttacaaag ttttcaggac ttctcctgtc catgtattcc cttatgtctt taagtggcat
     1981 atccagttcc ttaagagtag cgatcatata aaaggtttca agctgcttat atgaatagta
     2041 tctgtaaccg ttccgggcaa cgatttccgg gctgagtata ccgatatcat catagtgaaa
     2101 aagagtctgc tttttgactc tgcatagttt tgcaaattct cccgtggtaa aatgatctct
     2161 catttttttc ttctcctatt gactatacgg ttaccgtata ctttaacata atcttgttgt
     2221 tttttcaata ctggtgaagg agcataacat ggaacatcta tttgataacg gagtaacagt
     2281 gaaaagattt ttcgggttcg tatctccttc ggtgataatg ctgctggtga tagggcttta
     2341 ttccgttatt gacagcattt ttattgccaa ctgtgtaaac acccatgccc ttgcagcgct
     2401 gaacattgtt tatcccgtat tcggttttat atggggagtt tctgtcatgc ttgcagccgg
     2461 ctcttctgct ctggtggcaa taaagatggg agagggaaaa tctctggaag ccaaccggaa
     2521 atttactctt atatatatag ttgcagctat tatagccctt gtgatgatac ttccggcttt
     2581 tatatttatt gacgaattac ttttgtttct gggagcttca gatgtacttt gggattactg
     2641 caggacttac ggaataatca ttatttttgc catgcctgca ggttttatag gtgtactgtt
     2701 cgagtttttt atacgagtcg acggaaaacc cggattcaca ctggctcttt atatagtcag
     2761 cggaacggtc catgtcgttc ttgatgttgt ctttcttgtt tatctggact ggggaattga
     2821 aggtgccgcc tgggccactt tcgccggaat gtattctaca gtaataatgg gtgcctgtta
     2881 ttttattttt cgcgatacca aactgaaatt cgttaagacc aaaatggact gggcctacat
     2941 tggtcattcc atgtcaaacg gctcttcgga aatggtttct gaatcatctg tgggaataat
     3001 ggtgtttttt ttcaactaca taatgatgga tcttgcagga gaaactggtg tagccgcatt
     3061 gaccatcgtt cttgagatac tttacttcat ggtttctgta tatcttggtt atgttacagg
     3121 cgtagcccct cttataagct attattacgg tgccgggaga tatgaaaaag tgaactgctt
     3181 tctaaaatat tcgaaaaact tcattctgtt ttcctcaata cttatttcgg cagtttgtct
     3241 tttctttgcc ccgatgcttt caggtgtttt tgttcctgcc ggcagccagg tctatgatat
     3301 ggccactacc ggcctcaggt tcatggccat aggatttctt ttcaccggca taaatatttt
     3361 tgcttccgga ttcttcaccg cctacggaaa cggaaagatc tccgcattga tatccctttg
     3421 ccgtggtctt gtactggtca tcgtcttagg atttttgctg tcatatcttt tcggcatcaa
     3481 cggtgtatgg cttgctcttg tcatgaccga tatcgtgaca gttatccttt catttgcaat
     3541 gtttaaaaga tacagagaaa agtatcatta ttctttcaga taggatcctc caaatatgaa
     3601 aatccgttct gtctttcttg actaagcgtg attaccgccg tataattttt tcatgagttt
     3661 atttgaattg cttctgacag ccaccggtct tgcaatggat gcttttgcgg tttctgtctg
     3721 caaaggtgtt acattaaaag aaaaaaccac gagatatgcc ataactgcag gtctttggtt
     3781 cggtatattc cagggactga tgccggcggc aggttatttt gccggttccg cattcagtgt
     3841 tctggtagac agtttcgatc actggatcgc atttattcta ctttctttta tcggcataaa
     3901 aatgatcgtg gaatcaagga aaaaagactg tccttcgaat ccgtctttca agtctgccat
     3961 tatgtttcct cttgccgtag ccaccagcat agatgctctt gcagccggtg tttcttttgg
     4021 gtttttaaaa gtggatatcc tgcaggcctc tctgattatc gccgccgtca ccttcgttct
     4081 ttcatttctg ggagttaaga tcggtcacat tttcggatgc aggttccgct ccggagctga
     4141 gctttttggg ggaatcgccc tgatagccat aggaacaaaa atacttgttc aaagctttct
     4201 ttgctgataa ataagaactt tttttctgtt cttaatattt ccggctgaaa ataatgtaaa
     4261 gcacaagata taaaaccacc tgcgcccctg ccacataaca aacaacactg gccgcaaaga
     4321 catatccgaa gaccagaagt atcagggctg ctatcacttc acaaaaaact gttatgtaaa
     4381 aaacaaaact actacttttg gatgctatgt actttatcct ctcttctgtc atggaaagct
     4441 catatttcct tcgcctctca tcgtttcttg taagaataaa gagctgtgca atttttagaa
     4501 gacttactat tacaagggcg ataccgatac ttttcagtaa atcatcttcc ctgtaaaatc
     4561 cggcaacaga tataccggcc cccagagcgg atattattcc gcagtatata agattttttc
     4621 tgatgatttt cattttctgt cctccctgtt ttttgttttt tcgttattga aatcaccggg
     4681 aacaaatata tcctctatag ttttttcaaa aaactccgca attttaaaag caagtggcag
     4741 agaggccatg tatcttccgt tttctatgga aataatagtc tgtctggtaa cagagactgc
     4801 atctgccagt tcttcctggg ttatctttct ttcctttcgc agttttgcta tattgttttc
     4861 caaagagaca cctcccgttc actcaaaaat aaagtaaagc acactttact ttatagtata
     4921 gtgtgcttta caaaaatgtc aagtatgttt tacaattttt atacaaaaat atactgctgc
     4981 attatcatat aacaaatggt acctgccact atactcagga tactgtttcc tattctgaaa
     5041 tgaagagcaa gaactactgc cgccgagata agctcgggga taccgaaggg atattccaaa
     5101 ggtgagatat tcctgaaaca gtatacgacc agcatgccca tcatggccgc cggcagtact
     5161 ttgctgagca aagtcatggc ttccggaact tctcttcctt ccggaaacaa tgcaaagggc
     5221 acccaccgca taatctgtac tcctatggta actgctgcca ccatcaatag catttcggct
     5281 gttgtcatat ccatatcttt ttcctcacaa atacagccat aagcatcaaa agcatggcag
     5341 gtatcataaa agttacacca tcaaaaacca gaagtgctgt taatgaagca agaacaccca
     5401 ttactgccgc agatctgttt tcttttttct tccagtattc tgtgaaaagc actatgaaaa
     5461 gagctgtcat ggcaaaatcc attcctgtgg tatcaaatgt tataagcatt cccgccagac
     5521 tgcctatagc ggttccggcg acccagtaaa aatatatcat cagtgacatg aaaaaataaa
     5581 accatttccg gtccactttt tggggcacgg tgcagtgact ttctgtggaa aaactttcgt
     5641 cacacaatgt atataccata aagcctttca gctttcccat atccttatat ttttcaagca
     5701 tggaaatacc gtaaaaaagg tgtctggcat tgatcataag agaaagaaca aggacctgca
     5761 tcggttcaaa cactgaagct gcaagaaccg gtactgctgc aaactgcata ctgccgcaga
     5821 aagcaacggc actcatcgct attgcccatg gagctgatat gcccgccgtt tccattagca
     5881 tgccgtatac cactccgaga gaagtgcttc ctgccattat tgagatcgta tttgggaagg
     5941 cagctttgaa agccctcttt ttttctccca tttcttttcc ctttcttttt gcttgctttt
     6001 ctatactatt caagatatca tgacagtatt gataagtaaa acaaatatat ttaattcttt
     6061 atataagttt tgctaatgta tttatgtagg agaaaaaaat ggataaatat tctgtttttc
     6121 taaagatagt cgaaacggga agcttcacaa aagctgctca ggatctgggt tatacccagt
     6181 ctgcagtgag ccagatcgtc cggtctctgg aaaaagagct tttctcatct cttctgttaa
     6241 gggatcgaaa cggcgcaact ctcacctcgg aaggaagaga ccttcttccc tatataagaa
     6301 gcttaagcaa ttctcaccgg cagcttacag aaaaagcaaa cgatctgcac gggctcaagg
     6361 gcggtactgt tcgcattggc acatattcca gtatgtcctg ccacgttctg ccgccgctga
     6421 tgaaaagttt tggcagcaaa tatccggaaa tcaaatttga acttctccag ggagactatg
     6481 atgccaccgc aaattggata aaaaccggac gggtggattt cggactgctt ccgccaaaag
     6541 cttttcctca tctggaaata acagtcctga agaaggacag gatgatggcg gttttgcctc
     6601 cggggcacca tctttcaaaa agaaaaaccg tttctctttc agatctggcg gatgaatctt
     6661 atatacacct tgaagagggc agtttcagtg aaccttttca ggcctttgat gagctcgggc
     6721 tttttcctca tatccgcttc cgtgtccatg atgattacac cataatgtcc atggtcgaaa
     6781 gtggtcttgg cgtctctgtc ctttctgaac ttgttttgaa ccgcacttca tataatatag
     6841 aaataaggga aactgatcct gccgttatca gaaccatcgg catcgcctgc aaaagccgcc
     6901 gggctctttc cgccgccaat aaagaattta taaattacat aatgaaagaa atcaaatgat
     6961 ccgcttttga tttttttctt tactacttaa cttttccatg atacaataat tatgcaataa
     7021 agcaagaaag gaactaatat cttgagaaga tcagcagctg taacagaatt ttacttttac
     7081 tactttgcct atggcaaagc tggttttgct gctgtgagat aagaagttca tgacttaata
     7141 aaaaagctga tactgtgaaa ctcttctcct caggcaaaac cggcctgggg agttttttta
     7201 tgacagacag gagaatgaga tgataattat tttgaaagaa aacccggaca aaaaacagct
     7261 ggagaatctt accgactggc ttaaaaccat gggccttgac atacattaca gccagggtac
     7321 cagtacaacg ataatggggc ttatcggtga tacttctgtt gtagatacag atctgataaa
     7381 tgccctggat atcgtagaaa ccgttaaacg cataacagag ccttacaaaa acgccaatcg
     7441 aaaattccat ccctttgaca cagtcgttga ggtgcccctg tccggtggcg gaactgtcaa
     7501 gataggagga ggaaactttc agatcatagc aggtccctgt tcagttgaat ccgaagaaca
     7561 ggtctgcacc atcgcaatgg aggtaaagga ggccggtgca gggctctgca gaggcggagc
     7621 gttcaaaccc cgtacttcgc cctatgcttt tcagggtatg agagaacagg gcataaatct
     7681 tctgctggaa gcaaaaaaaa gatccggtct tcccattgtt acagaagtaa tggatatttc
     7741 tcatctgccc ttttttgaaa atgtggatgt tatacaggtg ggtgccagaa acatgcagaa
     7801 ctttgaatta ctgaaagaac tgggtcacac aggcaaaccg gttttactga agcgcggcct
     7861 ttcaagcaca ttaagagagt tgctcatgag cgccgaatac gtgatggcag gcgggaacag
     7921 caacgttatt ctgtgcgaaa gaggaattag aacctttgaa acggccacaa gaaatacgct
     7981 ggatctctcc gctatacccc ttttaaaaga aaaaacacat ctccctgtta tcgtagaccc
     8041 cagtcatgcc acaggatcag ccgcccttgt tatgcccatg gcaatggccg ccacggcagc
     8101 aggcgctgac ggtcttatga tagaagtaca taatgacccg gaacatgccc tttgtgacgg
     8161 agctcaatcc ataactccgg ccactttccg tgacattgta aaatccgtaa aaagcatact
     8221 gcctctggca tataaaggag gaaaaccatg ataggcgtta taggactcgg tctaataggc
     8281 gggtctctgg caaaagcact taatcagaat accgattatt ccgtatatgg atatgacatc
     8341 gacagatccg tcttgagcaa agcaaaactt gtgggtgcca tagaagacga actcaatgaa
     8401 aacatgcttt gcgaatgcga tatagttatt gttgcccttt atccgtcaga cactgttgac
     8461 tatgtcagat ccaatgcgga aaatttcaaa aaaggatcaa ttgtaataga ctgctgcgga
     8521 gtaaaaaaac ccgtttgcag cattcttgaa ccgctggcac aaaagcacgg ctttattttc
     8581 ataggaggtc atccgatggc agggcttgaa cattccggtt ttgagtattc aaaaaaagca
     8641 ttgtttgaca aggcctccat ggtactggtg cctccggcgg gcatcgggat aaagcatctg
     8701 gaaaaaataa aaggcctgtg ctcttcaatc ggcttttcga atatacagat atcgactgcc
     8761 gatgaacacg atcaaatgat agcctttact tcacagttgg cacacattac ttcaaacgca
     8821 tatataaaga gcagcgctgc catccgccat gccggatttt cggcaggcag ctactatgat
     8881 atgacccggg tggcaaagct caacgaaaaa atgtggacag aacttttcat aaacaatccc
     8941 gaatatctgt cctgcgaaat acaggaactc atagatcatc ttaacgaata taaaaacgcc
     9001 atagattcta aagacaaaga gaaacttaca gctcttctaa aggacggccg tgaaaaaaaa
     9061 gaaaaaatag ataacgaatt tttctaggga gtatttttga aatgaacaat ataataataa
     9121 aaaccgggat cccttatcag gtaataatgg aagaagactc tttagaaaaa gtcggctttt
     9181 ttgcttctga tataacagag ccctgcagga tctgcatcat atccgatgat aatgttcaca
     9241 gcctttatac acctttggtg gaatcttctc tgaaaaatgc cggtttcgat gtttataaga
     9301 tagtttttcc ggcgggagag gctacaaaag atctgggttc tgtgaaaaaa atacttgaat
     9361 accttgcaga tgagaatttc acgaggtcag atgtacttat ggctttggga ggaggcgtta
     9421 taggagatct tacaggattt gcggcttctg tttttttgag gggcataaaa tacattaatg
     9481 tgcccacctc tttccttgct gcagcagact cctctgtagg aggaaagacc ggcgtaaacc
     9541 tttctgcagg gaaaaatctg gcgggaactt tctggcaacc ttccctggtg atttatgacc
     9601 ccaaaactgt aaaaaccctt tctccggcat cttttcttga cggtcttgca gagtcaataa
     9661 aatgtgcatt tattgcagat gcttctcttt tcggtttcat agatctgagt gatatgaata
     9721 atgaaacgaa atttgtggag cgctgtgtaa cagattctgt aaaggtgaaa caattctttg
     9781 ttgaaaacga cgaatatgat aagggacgac gtcagcttct gaatttcggg cataccgccg
     9841 gacacgcaat agaaaaactg agcggattca gagaatctca cggacatgcg gtggctcagg
     9901 gcatggttat cgctgcaaga gcctcataca ataaggggtg gtcaagggaa gactgctgtt
     9961 cgcctcttat tgaggtgctg aataaatacg gtctttcttc ttcatgctcc tattcggcag
    10021 cagaactggc agcagcagct ctcaaagaca aaaaacgtct gggaaacgaa atcaatctgg
    10081 tcatacccct tgagatcggt gaatgtgctc ttatgcgact gccttcccat gaacttcaaa
    10141 atttctttga agcaggactt actggaagat gatttttcga taaaacacgg agactctata
    10201 tggatataat gataatgccc aaacccctaa aaggtaaaat aaaggctgtt gattcaaaat
    10261 cccatgtgca tcgccttctt atcgccgcag ctcttacaaa ggaccctgcg aaagtggtca
    10321 cagtaaccac atccgatgat atcgaggcaa caaggagttg tcttgccgag cttgaaaatg
    10381 atcaccctgt ttttcaatgt aaagaaagcg gctctactct cagattcatg atgcccattt
    10441 ccatggcgct taaggaaaga gctgttttca gatgtaaagg ccgtctgccg gaaagacctg
    10501 tctctccgct caaagaagcg atggaagctc acggatgctc tttctcaaaa agaatggacg
    10561 gatgggagct ggatggacgt cttggaagcg gagattattt tataccggga gatgtgagtt
    10621 cccagtatgt taccggtctt ttgttcgctc ttcctcttct tgccggtcac agtgccataa
    10681 gacttacttc tccgcttcag tctgaaagtt atgtagatat gactataaag acccttgagg
    10741 attccggtat aaaaatagaa acaagagcgc cggcaaagga tggatcatgc aatactgcgg
    10801 ccggagaatt tccgggaggc agttaccttg ttccgggcag acaaaaatac cgggctcctt
    10861 tatctgtccg tcccgagggc gactggtcaa acagtgcctt ctggtatgcg gcaaacaaaa
    10921 tgtcttcacg gaacaatata gtatgtacag gcctttcatc cggatccctt cagggggaca
    10981 aaaaaatcgg aaaatttctt tctttactgc cgggaaatat tgatgtttcg gacacccccg
    11041 atcttgtccc tcccattgct gctgctgcgg cattgacctc gggaaccact ttgataaaag
    11101 gagcctcaag gctcagacta aaagaaagcg accggcttcg gacggtttca cagacccttt
    11161 ccgatcttgg tgccgatata aaagagcttt ctgaaggtct tgagataaaa ggcaaaaata
    11221 tccttaccgg cggtacagta aacggatcag gagatcaccg tatcgttatg atggcggcca
    11281 tagctgcctc agtatgccag acacctgtaa taataagcgg tgcgcaggct gtaaacaaat
    11341 catatccatc cttctt
//
LOCUS       DAONHK010000029        11141 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_4589_length_11141_cov_5.025167, whole genome shotgun sequence.
ACCESSION   DAONHK010000029 DAONHK010000000
VERSION     DAONHK010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 11141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11141
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4589_length_11141_cov_5.025167"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="PLM92_02010"
     CDS             <1..346
                     /locus_tag="PLM92_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPR23947.1"
                     /translation="AASFEDCFVITVDSPLVSTETMRKMLDEHIRGENEVTLLKHGEK
                     TEPLIGIYKREFYRKIYPVIQNGSAPVFRALDRADVTNLEIDGIEKEILNINTKDAYE
                     AFLKENENNCAV"
     gene            324..1127
                     /gene="fdhD"
                     /locus_tag="PLM92_02015"
     CDS             324..1127
                     /gene="fdhD"
                     /locus_tag="PLM92_02015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HPR23948.1"
                     /translation="MKIIAQYENFPVSKPESIKKMFKGSDDIKEHPDPVIQEHFMDVY
                     VNGVFCFDLCSSPCYLEQLVTGHLLAQGHIKNIKDIEKIEIKGLAAKADVTLAKGRVT
                     EMPVELKDAVRIAMGGGEKAKKAGLTPLSLVKNRITGDRERRKEIFIQAETLYESSEV
                     FFETGAAHSSSLAFDGEIKYSFYDVSRHNTVDKVIGQALIDGTDLSKAVLFTSGRIPL
                     DMIVKAVRCRIPIVCSRSAPSGEALKMARICNVSLAGFVRGERMNIYND"
     gene            complement(1176..2174)
                     /locus_tag="PLM92_02020"
     CDS             complement(1176..2174)
                     /locus_tag="PLM92_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003365418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGGtGRT protein"
                     /protein_id="HPR23949.1"
                     /translation="MTLFENYERRIDKVNKVLANYSISSLKEAREICTLKGFDPHQIV
                     KDVQPIAFDDAFWAYTAGAAIAIKEGCTTAKKAAEAIGHGLQAFCIDGSVAEERKVGL
                     GHGNLGAMLLSEETNCFAFLAGHESFAAAEGAIGIAKSANKSRKKPLRVILNGLGKDA
                     ALIISRINGFTYCQTKFNYCTCKIEILKETAYSDGERSKVRCFGADDVREGVAIMHME
                     TVDVSITGNSTNPTRFQHPVAGTYKKECVLAGKKYFSVASGGGTGRTLHPDNMSAGPA
                     SYGLTDTMGRMHSDAQFAGSSSVPTHVEMMGFIGMGNNPMVGATVAVAVAVEEAMK"
     gene            complement(2190..2882)
                     /locus_tag="PLM92_02025"
     CDS             complement(2190..2882)
                     /locus_tag="PLM92_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003477845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPR23950.1"
                     /translation="MIYSKEVSDMCPVRNNANHGPSPIPEEGKWIKAREIKDISGLTH
                     GVGGCAPHQGACKLTLNVKEGIIQEALIETIGCSGMTHSAAMASEILPGKTILEALNT
                     DLVCDAINTAMRELFLQIVYGRTQTAFSEGGLPVGAGLEDLGKGLRSQVATTYGTAVK
                     GPRYLDIVEGYVTRIGLDKNDEIIGYEFVHIGKMMAAIKKGTEPAKAVKDASGKYGRF
                     DDAVRYIDPREE"
     gene            2984..5230
                     /locus_tag="PLM92_02030"
     CDS             2984..5230
                     /locus_tag="PLM92_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013149961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HPR23951.1"
                     /translation="MTYLDDLNKKQLEAVMYTEGPLLILAGAGSGKTSTMTHRIAYLI
                     KEKGVDPYNILAVTFTNKAAGEMRERVEKLLGGPNPVWIMTFHAACLRILRTETGLSG
                     YTRDFAVYDPVDQKAVIKSIIKEDNIEEKDFPYRMLISVISKCKEAQHSPEDYRRIHE
                     KNLKTETIYNVYKKYEEILKKNNAMDFDDLLLNAVKLFEENEEIILKYRTRFRYVMVD
                     EYQDTNRIQYRLIKMLAQEHKNLCVVGDDDQCIYQWRGADISNILNFEKDFPGTKVVK
                     LEQNYRSTGNILKAAYSVIKNNIKRKPKMLWTESEEGSKIIYCRADDEKAEAHYVAQE
                     IDRLCGPGKSFSDFAVLYRTNVQSRTFEEAFSSRDIPYRVVGGTRYYERKEIKDMMAY
                     MRLVQNPDDDLSLARIINEPKRGVGQKTLDKLKALASVRGQSVFSVLGDEEIMASLSG
                     RTGRELKQMHDAVESYSIEKNNLRVSDIYDGLLVASGYIKALEAQKTTEADGRIENLM
                     EFKSVIYDYEKETIGEEVSLTGFLEKIALLSDVDNHDPSENAVVLMTLHSAKGLEFDF
                     VFMPGMEDGLFPGWRKFDETDEMEEERRLCYVGMTRAKERLCFTSAAYRTMYGRGDYT
                     RESSFLRELDPKLTEGDAIFRKREEIKLGEKVDLDGFSKEEPLRPFEERFSGSGNSEM
                     LRKAVSEVHEKSEKKENFIVGDKVAHSKFGPGLVVSSRGKVITVIFDKAGTKKLAADI
                     APLRKIEE"
     gene            5249..5593
                     /locus_tag="PLM92_02035"
     CDS             5249..5593
                     /locus_tag="PLM92_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HPR23952.1"
                     /translation="MMAAKVREINLESGMPDGGQAMKRMMNGLTTAKQSGCRAVILIH
                     GYGSSGAGGIIKKEVGSKLRDPSLRGIVRDNVKGENWIIKKKEFLDICPALREFERHI
                     DGNNGITVVILK"
     gene            5590..6480
                     /gene="pgeF"
                     /locus_tag="PLM92_02040"
     CDS             5590..6480
                     /gene="pgeF"
                     /locus_tag="PLM92_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002603972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HPR23953.1"
                     /translation="MNGIIWKKKNSTGTLFTREENGVYYLGFKALAKTGLVKHGFSTR
                     LGGVSEGCYSTMNLSFTTGDKKENVEENLRRMGDALGTMPSGMTAPYQAHTVNVRRVG
                     ERDKGKGITREKDPEETDGLMTDVPGLMLVTGHADCLPLYIVDTERRAIALSHSGWKG
                     TAHRIGQMTLLAMKKEFGTQPENVIVGIGPGICEECYEVGENVAVEVRMNFNKESWHE
                     ILIQKKEFHKGSLDHNAEGHKYMMDLSRANEIIFEEAGVKKENIHTADICTMCNSDLL
                     FSHRKMGKKRGGQAAFLCLE"
     gene            6548..7777
                     /locus_tag="PLM92_02045"
     CDS             6548..7777
                     /locus_tag="PLM92_02045"
                     /inference="COORDINATES: protein motif:HMM:NF015400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HPR23954.1"
                     /translation="MKKILVLGVGAQGSAAAKKLDEEPIVSRVVCADYDREAVDHLVG
                     ELSKGEGKVVDAHDKGSIVAAAKGADLILNALPLECTKNVLEAALEAGTDYQDYAGTT
                     ALHEDWVENYRIQFDEYGPRFKAIGRTAVIGTGSAPGLICAATRDAMKYLDTCDTILN
                     IVWEGVITKRFLPFWWSPVTALHDMSEDAYAFENGKLVRTPAFGMTINRKYPYMENEI
                     TFAEHCHDEPIHYSFNAEKYFKGVKNAYFKYAGEGMDFCRPLARAGLLSHKKESIKGV
                     EVAPFDVVLAHLPHPPKFREEIKEIIEEGLISDSGCMVIEAYGKKDGKDVKVETHVMA
                     PGLLESYEKAGITAEMYLTGQGGYLFSKLFINDEFKIPGLFTSDMLTMEQVDLYFEYA
                     KELGITLETNITEIQKK"
     gene            7788..9800
                     /gene="ligA"
                     /locus_tag="PLM92_02050"
     CDS             7788..9800
                     /gene="ligA"
                     /locus_tag="PLM92_02050"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HPR23955.1"
                     /translation="MDDKKSIQQEIRERTAFLKKAAKAYYRDNEEIISNFRYDKLYDE
                     LLALENETGIIMAGSPTRQVGYETLSDLVKEAHPKKMLSLDKTKEPEELISWLGERKG
                     ILSWKLDGLTIVLTYKEGKLFKAVTRGNGEVGEVITNNARVFLDLPLEIPFKGETVLR
                     GEAVITYSDFQKINESIPELQAKYKNPRNLCSGSVRQLNNRITAERNVRFRAFALVST
                     ALSDGTKANIEDLPDFGGSKEKQMKWLENQGFYVVGYRVVDAKNLKGTIDWFGRNIKA
                     NDIPSDGLVLSYDDIEYGRTLGETAKFPRDSMAFKWRDETATTKLMEIEWSASRTGLI
                     NPIALFEPVQLEGTTVSRASLHNLSIVEDLKLGVGDEIEVYKANMIIPQIAANYTKSG
                     SIAPPQKCPVCGGRTEIRDENQVRTLYCTNPKCPAKKTKAFSHFAGRNAMNIDGISES
                     TIEKFTAKGFIKEPADFFLLDRHRKEIEEMEGFGERSYKNLSAAIEKARETTASRLLY
                     SLGISGIGSANAKLIAKHCKNDWQRIESLSKDELTKINGIGEVMADAFVRYFADDRNI
                     KKVRELIKEIRLEPPEELPETGNQDLEGMSFVITGGLNKFENRDELKDLIEKRGGRTS
                     GSVSTKTTALINNDMNSGSAKNKKAAELGVRVISEEEFLKEYDIDA"
     gene            9793..10698
                     /locus_tag="PLM92_02055"
                     /pseudo
     CDS             9793..10698
                     /locus_tag="PLM92_02055"
                     /inference="COORDINATES: protein motif:HMM:NF014788.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase family protein"
     gene            10781..11021
                     /gene="ffs"
                     /locus_tag="PLM92_02060"
     ncRNA           10781..11021
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLM92_02060"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
BASE COUNT         3586 a         2146 c         2769 g         2640 t
ORIGIN      
        1 agccgcttct tttgaagact gctttgttat aactgtggat tctccccttg tttccaccga
       61 gactatgagg aaaatgcttg atgaacacat caggggcgaa aatgaagtta ctcttcttaa
      121 gcatggagaa aaaacagaac ccctgatagg tatatacaaa agagagtttt acagaaagat
      181 atacccggtc atacaaaacg gatccgcacc ggtttttagg gctcttgaca gagcagacgt
      241 gacgaacctt gaaatagatg gaattgaaaa agagatactg aacatcaaca caaaagatgc
      301 atatgaagca tttttgaaag aaaatgaaaa taattgcgca gtatgaaaat tttccggtat
      361 caaagccgga atccataaaa aaaatgttca aaggcagcga tgacataaaa gagcatccgg
      421 atcctgttat acaggagcat tttatggacg tatatgtcaa cggtgttttt tgttttgatc
      481 tttgctcatc gccttgttac cttgaacagc ttgtaacagg gcatcttctg gcccaggggc
      541 atataaagaa tataaaggat atagaaaaga ttgaaataaa aggtctggcg gcaaaggcag
      601 atgttactct tgccaagggc agagtcactg aaatgcccgt cgaattaaaa gatgcagtga
      661 gaatcgcaat gggcggaggc gaaaaagcaa aaaaggcagg gcttacgcct ctttcgttgg
      721 tgaaaaatag aataacagga gacagggaac ggagaaagga gatattcata caggcagaga
      781 ctctttatga atcttcggaa gtttttttcg agacaggagc agcacacagt tcttcactgg
      841 cgtttgacgg ggaaataaaa tactcttttt atgatgtcag ccgccataat acagttgaca
      901 aagtcattgg gcaggcactt atagacggaa cggatctttc aaaagcagtt ctttttacca
      961 gtggcaggat accccttgac atgatagtta aggccgtgag atgcaggata cctatagtat
     1021 gctccagatc tgcaccctca ggagaggctc ttaagatggc gcgaatatgt aatgtgtctc
     1081 ttgcagggtt tgtcagggga gagagaatga acatatataa tgattaaaaa aggccgcaga
     1141 aaattattct gcggtctttc tgaaatacaa gatgtctatt tcattgcttc ttccacagcc
     1201 acggctacag ccacggtggc gcctaccatc gggttgtttc ccataccgat gaatcccatc
     1261 atctcaacat gtgtgggaac ggaagaagaa cctgcgaact gggcatctga atgcatgcgg
     1321 cccatggtat cggtcaggcc gtaagaagca ggacctgcgc tcatattatc gggatgcaga
     1381 gttctgcctg tgcctccgcc cgaggccaca gaaaaatatt ttttgccggc aagaacacat
     1441 tcttttttgt aggtacccgc cacgggatgc tggaaacggg tagggtttgt ggaattcccg
     1501 gtgatagaaa catccactgt ctccatatgc attatggcta cgccctctct gacatcatca
     1561 gccccgaaac atctcacctt gcttctctca ccatcggagt aggctgtttc ttttagtatt
     1621 tctatcttgc aggtgcagta attgaacttt gtctggcaat atgtgaatcc gtttatcctt
     1681 gagatgataa gagcggcatc tttgccgagt ccgttaagta tgacgcgaag cggttttttt
     1741 ctcgatttgt tggcactctt tgcaatgcct atggctcctt ctgccgcagc aaatgactca
     1801 tggcccgcaa ggaaagcaaa gcagttggtt tcttcggaca aaagcatggc tcccaggttg
     1861 ccgtgaccca gaccgacttt tctttcttcg gccacagagc cgtctatgca gaaggcctga
     1921 agaccgtgac caatggcttc ggccgctttc ttggcggtag tgcagccttc ttttattgcg
     1981 atggcagcac cggcggtata agcccagaag gcatcatcga aggcgatcgg ctgaacatct
     2041 ttgacgattt gatggggatc aaaacccttg agagtgcaaa tttcccttgc ctctttaagg
     2101 gagctgatac tgtaatttgc cagcactttg tttactttgt ctattcttct ttcataattt
     2161 tcaaataaag tcatgatcag tcctccgttc tactcttcac ggggatcgat ataccttacg
     2221 gcatcatcga aacgcccgta tttgcctgaa gcatctttga cagcttttgc cggttccgtt
     2281 ccctttttta tggccgccat catcttccct atatgaacaa attcataacc tatgatctcg
     2341 tcatttttat caaggcctat tcttgtcaca taaccttcaa caatatcaag gtatctgggg
     2401 cctttgaccg ctgtgccgta tgttgtggcc acctgacttc tgagcccttt gccgagatcc
     2461 tcaagacctg cgcccacagg cagtccgccc tccgagaagg ctgtctgtgt ccttccgtat
     2521 acgatttgaa ggaaaagttc ccgcatggcc gtgtttattg catcgcaaac aagatccgta
     2581 tttaaggctt ccagtatagt tttgcccggc aggatctctg aagccatggc agcagaatgg
     2641 gtcatgccgc tgcagcctat ggtttctata agagcttcct gtattatacc ttctttgaca
     2701 ttaagggtaa gcttacatgc tccctggtgt ggtgcacatc ctccgacgcc gtgggtgagg
     2761 ccgctgatgt ctttgatttc tctggccttt atccatttgc cttcctcggg tataggagac
     2821 ggtccgtgat ttgcattgtt tcttacagga cacatatcgc ttacttcttt tgagtagatc
     2881 atttaagcac tcctctctga tcacatatat atgctgcttg gttaccataa aaattatatc
     2941 atattagttt cagcaattcc atattatatg ttagaataga accatgactt atttggatga
     3001 tttgaacaaa aaacagcttg aagctgtcat gtatacagaa ggtcctctgc tgatactggc
     3061 aggagccggc agcggcaaga ccagcacaat gactcacagg atagcatacc ttataaagga
     3121 gaaaggtgtt gatccttaca atatactggc ggtgactttt acaaacaagg cagcagggga
     3181 aatgagggaa agagtggaaa aactcctcgg aggccccaat cctgtctgga taatgacatt
     3241 tcatgccgca tgtttaagga tactgagaac agaaaccggc ctttccggat acacccggga
     3301 ttttgccgtg tatgatcccg tagatcagaa agcagttata aagagcataa taaaagaaga
     3361 caatatagaa gaaaaagact ttccttaccg tatgttgatc tctgtgatca gcaagtgcaa
     3421 ggaagcgcaa cactcaccgg aggattacag gcggatacac gaaaagaatt tgaagacaga
     3481 gaccatatac aatgtatata aaaaatatga agaaatattg aagaaaaaca atgcaatgga
     3541 ctttgacgac cttcttctca atgccgtaaa gcttttcgaa gaaaatgaag agattatttt
     3601 aaaataccgg accagattcc gttatgtgat ggtggatgag taccaggaca caaacaggat
     3661 acagtacaga ctcataaaaa tgctggccca ggagcacaaa aatctttgtg tggtaggaga
     3721 tgacgatcag tgcatatacc agtggagggg agcggatatt tccaatatac tcaactttga
     3781 aaaggatttt ccgggcacaa aagtcgtcaa acttgaacag aattacaggt ctacaggcaa
     3841 tatattaaaa gccgcttatt ctgtaataaa aaacaacata aaaagaaaac ccaaaatgct
     3901 ttggaccgaa tcagaagaag gaagcaagat aatatactgc agggcagatg acgaaaaagc
     3961 ggaagcgcat tatgtagctc aggaaataga caggttatgc ggcccgggaa agtcattcag
     4021 tgactttgct gtactttaca gaacgaatgt acagtccaga acatttgaag aggcattttc
     4081 ctccagagat attccctaca gggtcgtagg cgggaccagg tattacgaaa gaaaagagat
     4141 aaaggacatg atggcttata tgagactggt gcagaacccg gatgatgatc tttctctcgc
     4201 acggataata aatgaaccca aaagaggcgt tggacaaaag acactggata aattaaaagc
     4261 tctggcatca gtgcgggggc agtcggtttt ttctgttttg ggggatgaag agatcatggc
     4321 ttcattgtcg gggaggaccg ggagagagct gaagcagatg catgatgccg tagaaagtta
     4381 cagtattgaa aaaaacaatc tcagggtttc tgatatatat gacggattac ttgtggcaag
     4441 tggctacatc aaagctcttg aagctcaaaa aaccacagaa gccgatgggc gcatagagaa
     4501 cctgatggaa ttcaagtctg taatatatga ttacgaaaaa gagaccatag gggaagaagt
     4561 ttcgcttacg ggattccttg aaaaaatagc attgctttcg gatgtggaca atcacgatcc
     4621 ttctgagaat gcggtagttc ttatgacact gcacagcgca aaaggtcttg agtttgattt
     4681 tgtttttatg ccgggaatgg aagacgggct tttccccgga tggcggaagt ttgatgaaac
     4741 tgatgaaatg gaagaagaaa gaagactttg ctatgtggga atgacaaggg caaaggagag
     4801 gctgtgcttt accagtgccg cttacaggac tatgtatgga agaggggact atacaagaga
     4861 atcctctttt ttaagggaac tggacccaaa acttactgaa ggggatgcca tattcagaaa
     4921 gagagaagaa ataaagctgg gagaaaaagt ggatctcgac gggttctcaa aagaggaacc
     4981 gttaagaccc tttgaagaga ggttttcagg atccgggaat tctgagatgc taagaaaagc
     5041 cgtcagtgaa gtacatgaaa agagcgaaaa aaaagaaaac ttcattgtgg gagataaagt
     5101 tgcccacagc aaattcgggc ccgggcttgt tgtttcctcc cggggaaaag tcatcactgt
     5161 aatatttgat aaagcaggaa caaaaaaact tgcggctgat atagcccctc tcaggaaaat
     5221 agaagaatag gatcggtaag gaaggaaaat gatggcggca aaagtcagag agataaatct
     5281 ggaaagcggt atgcccgatg ggggccaagc catgaagaga atgatgaacg gccttacgac
     5341 cgcaaagcag tcaggctgca gagcggtgat acttatacac ggttatgggt cttcaggagc
     5401 cggcggcatt atcaaaaaag aagtcggatc aaagcttagg gatccatccc taagagggat
     5461 agtaagagat aatgtaaagg gcgaaaactg gatcataaaa aagaaagagt ttcttgatat
     5521 ctgtccggct ctcagggagt ttgaaaggca tatagacggg aataacggaa taacggtggt
     5581 gatccttaaa tgaacgggat aatttggaaa aagaaaaaca gtacggggac tcttttcaca
     5641 agagaagaaa acggcgttta ctatcttggg ttcaaagcac tggcaaaaac gggactggtg
     5701 aaacatgggt tttcaaccag actgggcgga gtaagtgaag gatgctacag caccatgaat
     5761 ctctccttca ccacagggga taaaaaagaa aacgttgaag aaaacctcag gcgaatggga
     5821 gatgctcttg gaacaatgcc atctggcatg acagcacctt atcaggctca tactgtaaat
     5881 gtaaggagag tcggggaaag agacaaaggg aaagggataa caagagaaaa agatccggaa
     5941 gaaacggatg gactgatgac tgatgtgccg gggctgatgc ttgtgacagg acatgcagac
     6001 tgtctccctc tttatatagt tgataccgaa agaagagcta tagccttaag tcattcggga
     6061 tggaagggaa cagcacaccg gatcggacag atgactcttc ttgcaatgaa aaaggaattc
     6121 ggtacgcaac cggaaaatgt gatagtaggt atagggcccg ggatatgtga agaatgctat
     6181 gaagtgggag aaaatgttgc tgttgaggta agaatgaatt tcaataaaga aagctggcat
     6241 gaaatactga tacaaaaaaa agagtttcat aagggttccc tggatcataa tgcagaaggt
     6301 cataaatata tgatggatct ttcaagggcg aatgaaataa tatttgaaga agcaggagtg
     6361 aaaaaagaaa acattcatac tgcggatata tgtactatgt gcaacagcga tttgcttttt
     6421 tcccacagga agatggggaa aaagagaggc gggcaggcgg cctttttgtg cttggaataa
     6481 attgacattt aatatataat aataatataa ctgacacagt atccgaatga agatttgagg
     6541 tgaaattgtg aaaaagatac ttgttctggg agttggggca cagggttctg ccgcagcaaa
     6601 aaaattggat gaagaaccaa tagtaagcag agtggtctgc gccgattatg acagggaggc
     6661 ggttgaccat ctggtgggag aactaagtaa aggagaaggt aaagtcgtgg atgcccatga
     6721 caagggcagc attgtggcgg cagcaaaggg agcggatctt atactgaacg cgcttccctt
     6781 ggaatgcaca aaaaatgtcc tggaggcagc ccttgaagca ggcacggatt atcaggacta
     6841 tgccggcacc accgcacttc atgaagactg ggtggaaaat tacaggatac agtttgatga
     6901 atacggaccc aggttcaaag ccatcggcag gacggcagta attggtacag gatctgcacc
     6961 tggactgata tgcgccgcca ccagagatgc gatgaaatat ctcgacacat gcgatacgat
     7021 attaaacatt gtatgggaag gagtaataac aaaaagattt ctgcccttct ggtggtcgcc
     7081 cgttacggct cttcatgata tgtctgagga tgcttatgcc tttgaaaacg gcaaactcgt
     7141 aaggactccg gctttcggta tgaccataaa cagaaaatac ccctatatgg aaaatgaaat
     7201 aacttttgcg gaacattgtc atgatgaacc catccactac agttttaatg ccgaaaaata
     7261 tttcaagggt gtcaaaaacg cttacttcaa atatgcggga gaaggaatgg acttttgcag
     7321 gccccttgcc cgggcgggac ttctttcaca caaaaaagaa agtataaaag gagtggaagt
     7381 tgcacctttt gatgtggttc tggcacacct gcctcatccg ccaaaattca gggaggaaat
     7441 aaaagaaata attgaagaag gccttatttc agattccgga tgcatggtca tagaggctta
     7501 cggcaaaaag gacggaaagg atgtcaaggt tgaaacgcat gttatggcac cgggacttct
     7561 ggagtcatac gaaaaggccg ggataacagc agagatgtat ctgacggggc agggaggata
     7621 tcttttttcc aaattgttta ttaacgatga attcaagatc ccggggcttt tcacttccga
     7681 tatgctgacc atggagcagg tggatcttta tttcgaatat gcaaaagagc tggggataac
     7741 ccttgaaaca aacataactg aaatacaaaa aaaatgaaag tgacataatg gacgacaaaa
     7801 aatccataca acaggagata agggaaagga cagctttcct caaaaaggcc gcaaaggcat
     7861 attacaggga taatgaagag atcatctcaa atttccggta tgataaatta tatgatgagc
     7921 tgctggccct tgaaaatgaa acaggcataa taatggccgg cagccctacc cggcaggtgg
     7981 gttatgagac tctttccgat cttgtaaaag aggcacaccc taagaaaatg ctttctctgg
     8041 acaaaacaaa agagccagaa gaactgatat catggctggg agaaagaaaa ggcatacttt
     8101 catggaaact tgacggtctc accatagtct tgacctacaa agaaggaaaa ctctttaaag
     8161 cggtaaccag gggcaacggt gaagttggag aagtaataac aaataatgcc cgggtattcc
     8221 tggatctgcc gctggagata ccttttaaag gagaaacggt tttgagaggt gaagcggtaa
     8281 taacttattc ggatttccaa aagataaacg aatctatacc cgaactccaa gcaaaataca
     8341 aaaatccgag aaatctctgc agcggttcgg taagacaact caataacagg ataacagcag
     8401 aaagaaatgt cagattcaga gcttttgcgc tggtcagtac cgcactttcc gacgggacga
     8461 aagcaaatat tgaagatctg ccggattttg gcggctcaaa agagaagcag atgaaatggc
     8521 tagaaaatca gggattttat gtagtcggat accgcgttgt agacgcaaaa aacctaaaag
     8581 gaaccataga ctggttcggc cggaatatca aagctaacga tataccctcc gacggactgg
     8641 tactttcata tgatgatatc gaatatggca ggactcttgg cgaaacagcc aaatttccca
     8701 gagattccat ggcattcaaa tggcgtgatg aaactgcgac caccaaacta atggaaattg
     8761 agtggagcgc atcaaggacc gggctcataa atcccatagc attgtttgag ccggtgcagc
     8821 tggagggtac gactgtgagc cgtgcatcgc ttcataatct cagtatagtc gaagacctga
     8881 agctgggtgt gggagatgaa atagaggtat ataaggcgaa tatgataata ccccagatag
     8941 ccgccaacta tacaaagagc gggagtatag cccctcctca aaaatgtccg gtctgtgggg
     9001 gcaggactga aataagggat gagaaccagg tcaggaccct ttactgcact aacccaaaat
     9061 gcccggcaaa gaaaacaaaa gcattcagcc attttgccgg tcgtaatgcc atgaatatag
     9121 atggtatttc ggaatccaca atagagaagt tcacggcaaa gggattcata aaagaacccg
     9181 ctgatttttt tcttcttgac agacacagaa aagagataga agaaatggaa ggattcggcg
     9241 agagatccta taagaacctg tctgccgcca tagaaaaggc cagggaaact actgcatcaa
     9301 gacttcttta cagcctgggg atatcaggaa taggctccgc aaacgcaaaa cttatagcaa
     9361 agcattgcaa aaatgactgg caaagaatag aaagcctttc aaaggatgag ctcacaaaga
     9421 tcaatggtat aggagaagtc atggcggatg ccttcgtaag atattttgcc gatgaccgga
     9481 acataaaaaa agtccgtgag ttgatcaaag aaatacgtct ggagcccccc gaagaattac
     9541 ccgaaacagg aaatcaggat cttgagggaa tgagttttgt tataaccgga ggcctgaaca
     9601 aatttgaaaa tagagatgag ctgaaagatc tcatagaaaa aagaggcggc aggacctcgg
     9661 ggtctgtaag cacaaaaaca acagcactta taaacaatga catgaattcc ggatccgcaa
     9721 aaaacaagaa agctgcagag cttggagtga gggtcatttc cgaagaagag tttttaaagg
     9781 agtatgatat agatgcttaa gataggcaaa cttgacagcg atcttctgga aaaaatagtt
     9841 ttcaggaaca tcaaattcaa aagaccggaa gtacttacaa gacccggtat cggagaagac
     9901 tgcgcagtaa tagactacgg ttcatatgaa tgtgttatgt caacagatcc aataacggct
     9961 gcaataggag atgtgggaag actttccgtt catatttcct gtaacgatgt ggcatccaac
    10021 ggtgttgaac ctgtaggaat aatgctgacg gtacttcttc ctgaaggcac caccgaaaaa
    10081 caggtggatg acatgatgca ccaggcggct caggcctccg aagagttggg tgtcgaaata
    10141 atagggggac acaccgaaat aaccaatgcc gtaaatcagc cggtaattgt ctccgctgcc
    10201 ataggaaggg gagaaaaatg ggcttctcag catactgaaa acatgaaaga aggagactat
    10261 atcctcatga caaaaaccgc gggcatagag ggaactgcca taatagcacg ggatttcgaa
    10321 aagcagctga agaatgttct cacaaaagaa gagataagtg aagcgatatc catgcttgac
    10381 aaagtgagtg ttgtcacaga aggtgttaca gcagggagga taggaacctc agggatgcat
    10441 gacataaccg agggaggcgt tttaggtgca ttgtgggaga tgtgccggat aggtaaaatc
    10501 ggtgctgaag ttttcatcga taaagtgccc gttgaagagg taacaaaaaa aatatgcgaa
    10561 gaatttgata tcgactacct gagactcata tcaagcggat gcatgatgat aatggcagga
    10621 cctgacaaaa aagatattct gatgagagaa ataagcgatg ccggtataca gataacctgt
    10681 ataggaaaga taaaaaaaga agagcatggc gttaagatga atgttaaggg agaactgata
    10741 gaaatagccc ctccggtcac cgatgaggtt tataaagccc tggcatataa aaagtaacag
    10801 ggcatgattg ccattgacag atcatacctg accatttaca attaagtaga aaaaatgccc
    10861 tttgacggct ccgcctggta tggtttgggt cctgcgcaat aggactctgt gaaccttgtc
    10921 aggtccggaa ggaagcagca ataagcggaa gctcttatgt gccgcagatc agccttctcc
    10981 tgcctgcgga gctttcaaag ggcgttttta tttataagcc gaaagaacgg catggcagtt
    11041 tatagtcata caaaagcaga aaaggaacga atatccatgt acaaagtttt gtatcgtgcc
    11101 tacagaccgg aagtcttcaa agagatgctg gggcaggagc a
//
LOCUS       DAONHK010000030        11093 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_4611_length_11093_cov_5.042490, whole genome shotgun sequence.
ACCESSION   DAONHK010000030 DAONHK010000000
VERSION     DAONHK010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 11093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11093
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4611_length_11093_cov_5.042490"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            80..1744
                     /gene="ilvB"
                     /locus_tag="PLM92_02065"
     CDS             80..1744
                     /gene="ilvB"
                     /locus_tag="PLM92_02065"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020005773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HPR23956.1"
                     /translation="MKFTGSQIVAEILLEHGVDTVFGYPGGAALNIYDALYEYRDQIR
                     HVLSAHEQGAAHAADGYARVTGKTGVVFATSGPGSTNLVTGIATAYMDSVPMVAITAN
                     VPSNLVGRDAFQEVYITGITLPVTKHNFFVNKIEDLPGALNDAFRIAGSGRKGPVLVD
                     ITKDVTAATIDYKSRKMPEEPKKVSGIDAEELKNIADIINKSKKPLVYLGGGVRAAGA
                     GEELGAFLKKGDIPAAHTIMAAGVLDADDPHNSGLIGMHGTIASNRAVDNADLLLAIG
                     TRFSDRVALNPKKFAKKATRVQIDIDKSEINKNVLVDIGMVCDAKDFFTALMPLIEKK
                     DRSEWVGEVMELKKTTGDVKCDDAELHPSEIIGTVCDMTDKETVYVTDVGQHQMWAAQ
                     YLKHDKTCCFLTSGGLGTMGFGYGASIGAQVGAPGKRVIHFTGDGSFHMNLNEACTAV
                     SQQLPIITIIMNNSVLGMVYQWQTSFYGSRFSATEPGRKTDFVKLAEGFGLKGFRASN
                     MDEFTDAFKKALKEKGPVWIECIIGKNEKVLPMIPAGGTVEDMIIG"
     gene            1758..2267
                     /gene="ilvN"
                     /locus_tag="PLM92_02070"
     CDS             1758..2267
                     /gene="ilvN"
                     /locus_tag="PLM92_02070"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007049822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HPR23957.1"
                     /translation="MALKKEVISIFVDNQSGVLARVVSLFGRRGFNIDSLTVSATNDP
                     SISRITIVFKGDDLSLAQIISQTEKLEVVRDIFTLEKEGSLFRELLLLKVKAGKEERT
                     TVKEIVDIYRGKIIDLSKESMIIEITGAPEKIDGLMNMLSCYEIVEVCRTGITAIERG
                     WQEKEKKND"
     gene            2260..3255
                     /gene="ilvC"
                     /locus_tag="PLM92_02075"
     CDS             2260..3255
                     /gene="ilvC"
                     /locus_tag="PLM92_02075"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008727498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HPR23958.1"
                     /translation="MIKKYYDQDCDLKLLDGKTIAVIGFGSQGHAHAQNLKESGCSVV
                     VGLRPDSKSAKSARDAGLDVMDVESAAEKGDIVMMLVPDEKAADIYNRQVAPHMKEGN
                     VLMFAHGFNIHYQLIKPDECLDVIMVAPKGPGHTVRSQYMEGKGVPSLIAVHHDRSGK
                     ARDYALAYASGIGAGRAGILETSYREETETDLFGEQAVLCGGVTELMKAGFETLVEAG
                     YEPEMAYFECIHEMKLIVDLINSGGFEMMRKSVSNTAEYGDYYAGKQIITEDSRKAMK
                     QILARIQNGEFASEFVSEMSSGRQARFLTTRMKENEHQLCKTGAELRKMMSWLKK"
     gene            3252..4916
                     /gene="ilvD"
                     /locus_tag="PLM92_02080"
     CDS             3252..4916
                     /gene="ilvD"
                     /locus_tag="PLM92_02080"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008978568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HPR23959.1"
                     /translation="MKERSDKITRGIERAPIRSLLYALGYTKEELERPLIGVISAHSE
                     IVPGHMNLDKITDAVKAGVRMAGGTPIEMPSIGVCDGIAMGHEGMKYSLASRELIADS
                     VETMIQAHMMDGMVLVPNCDKIIPGMVMAAVRVNIPTVVCSGGPMMAGNVDDEEVSLS
                     KIFEAVGARKAEMIDDDELTRYEQGVCPGCGSCAGMYTANSMNSLCEAIGIALPGNGT
                     IPAVHSDRLRLAKHAGMAIMDLLKNDIKAKDIINKKSIGNALACDMALGCSTNSVLHL
                     LAITHEAGIELDLSVFNEYSAKVPNLCHLAPAGPHHMQDLNKAGGIQAIFSELDKKGI
                     IDRDVITATGKTVGENIKGIKVKDSKVIRPIDDPYSETGGLAVMWGNIAREGCVVKRS
                     AVSPEMLVHKGPARVFDSEDEAINTIYEGKINDGDVVVIRYEGPKGGPGMREMLNPTS
                     ALAGMKKDKTVALITDGRFSGASRGASIGHVCPEAASGGEIGLIKEGDIIEIDIPSAK
                     LNAMVSDEEFAERKKQQKMPASSVTKGWLVRYRRNVAGACKGAVME"
     gene            4958..5794
                     /locus_tag="PLM92_02085"
     CDS             4958..5794
                     /locus_tag="PLM92_02085"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008397300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HPR23960.1"
                     /translation="MKMKIGCHLSAAGGYEKMGREALFIGASTFAFFTRNPRGARSKE
                     TDEKDIGALRKIMEDKEFAPLVAHAPYTLNPCSADKKVRDFAFFVMEDDLKKMQYLPG
                     NYYNIHPGNHLGQGIEKGIELTAELLNKIIRPEYETTVLLETMTGKGTEIGSVFGQLS
                     DIIERVRQKNKVGVCIDTCHISDAGYDIKNSLDEVIDEFDRTIGLKYLKAMHINDSLN
                     IMGAKKDRHARLGEGILGLDTIINVVNHPDLKALPFILETPNELEGYKREIELLKEAY
                     KG"
     gene            5791..7065
                     /gene="hisD"
                     /locus_tag="PLM92_02090"
     CDS             5791..7065
                     /gene="hisD"
                     /locus_tag="PLM92_02090"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HPR23961.1"
                     /translation="MKKLKEAKTRNMTNEKNLQKDVFEIIEKIRTEGDAAVLSYNEKF
                     DGSKRTDLRISKKEIDEAYTLTEKEDPCFISDIKKAAANIKAFAQKQMETFTDIDEFS
                     PAEGIKLGHRIIPLSSCCCYVPGGNYPLYSTALMLCIPAKTAGVKRVTACSPPIKGSG
                     KISPKTIVALDTAGADEIYALGGAQAVAAFSYGTCQIKPVDLIAGPGNRYVTEAKRQC
                     YGQVGIDFLAGPSEVLIIADETAEAEIIAADILAQSEHDIMAKGIALVTDERKGKEII
                     EAVKRQLKDLKTADIAEKAWKDNGEVLVFDDLDEIIEYANDHAPEHLEVFVAERDEKD
                     LCKKLKNYGSLFVGRNAAEVFGDYATGTNHTLPTGRAARYTGGVWIGTFLKVCTHQRL
                     TPEGMNDISDLVSRLAEGEGLFAHAKAAKIRK"
     gene            7199..8782
                     /gene="hisG"
                     /locus_tag="PLM92_02095"
     CDS             7199..8782
                     /gene="hisG"
                     /locus_tag="PLM92_02095"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016322280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HPR23962.1"
                     /translation="MEFETADLKPEEIVTLKLRGIFRKQGYKKYKMSRFEEYGLYAAN
                     KDFLGGDKVITFTDLDGRLLALKPDVTLSIIKNTRADLSSQEKLYYIENVYRESKESH
                     TFKEIPQVGLEFIGKVGKEGIIETVCLAAETLKTVSRNYVLEISHMDFAVELLQSMDL
                     NKEEYFHILRLIRSKNADGIKTASEKAGLTEKQTEKLCKLTELYGSVQKTLNSARKLV
                     INDTMEDALVQLEKVCRGISASGITGRLQIDLSMVNDIDYYNGIIFRGYLKNHRSNVL
                     AGGQYDKALRLFGKKAEGIGFALYLNEISREEKIKRQTSKNKKHQMLSIALPKGRLGS
                     RVYKLLEQAGYGCPGYDGKNRKLVIENKQKGIRYLFVKPADVAVYVEHHAADLGIVGK
                     DIIMETEPDVYELMDLKTGICSMVTAAPKGYKEDTDRTLKVATKYVNVAKQYYLEKNR
                     DIEIIKLNGSIELAPILGLSDIIVDIVETGSTIKENDLEIIEGFRQISAFLIANRSSF
                     KFCNKMITEMTERLRGIIG"
     gene            8779..9825
                     /gene="hisC"
                     /locus_tag="PLM92_02100"
     CDS             8779..9825
                     /gene="hisC"
                     /locus_tag="PLM92_02100"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006354328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HPR23963.1"
                     /translation="MSRFVRKDFLALMPYSPGQQPEEKMIKLNTNENPYPPSPGAVDA
                     IGKISTDELRLYWDPEASEAKNAIAEHYDIESSRVMVTNGSDEALAFAFHAFGEKICF
                     PEITYSFYKVFAMATGSHAVTFPLKKDMIIDITDYKKFSGTVILANPNAPTGIALCRE
                     QIREIVLSDPDRLVIIDEAYVDFGAESCISLVKEYDNILVIQTMSKSRSLAAMRIGFA
                     LGSRDVIADLNKIKYSFNPYSMDPFATAAAKAAIEDEAYFIKTTAEIIRTREKTAAGL
                     RDLGLEVIPSKTNFLLVSGGREYFEKLKEKKILVRYWDENFLRNWVRISIGTPGQMEK
                     LLKATKEIICEKVK"
     gene            9807..10430
                     /gene="hisB"
                     /locus_tag="PLM92_02105"
     CDS             9807..10430
                     /gene="hisB"
                     /locus_tag="PLM92_02105"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015822909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HPR23964.1"
                     /translation="MRKSEIKRKTAETDISISLDIDGNGNYDIRTDCGFFDHMLELFT
                     RHGRFDVSLKCKGDSHVDYHHTVEDTGIVMGRAFREALGDKKGIKRYGSMIMPMDEVL
                     MLCALDISGRCFLNFGVEFKVMKVTSGETEERPMMAGLFDTELVSVFLDAFCRELGLT
                     LHVMKLSGENTHHIIEAVFKGLGRALASAAEIDPKAKNEIPSTKGVL"
     gene            10430..11053
                     /gene="hisH"
                     /locus_tag="PLM92_02110"
     CDS             10430..11053
                     /gene="hisH"
                     /locus_tag="PLM92_02110"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HPR23965.1"
                     /translation="MIAVADYGVGNLFSLCASLKYLGIDTEVTKDPKKIKEAERIILP
                     GVGAFGDAIKKLEECGLSEVIKKEAKKGKPLLGICLGMQLLFERSLEYGIHSGLGLIE
                     GEVRSIADDMPKEARSGFKVPHIGWNRLDIDESEPLFKYINNGEYVYYVHSFYAGKCT
                     ENTIASSYHGIKITGACRRGSIWGTQFHPEKSGRVGLDILKAFGSVK"
BASE COUNT         3709 a         2051 c         2828 g         2505 t
ORIGIN      
        1 cattgtatca caaagggttg gtgcctcatt tttcattctt aactttacag accggcattt
       61 actgaaagga gatatcaaaa tgaaatttac aggctctcag atagtggccg aaatacttct
      121 ggagcatggt gttgatacag tgttcggtta tccgggagga gcagcgctga atatttacga
      181 tgccctttat gagtacagag atcagatacg gcatgtactt tcggctcacg aacagggagc
      241 cgcccatgcc gccgacgggt atgccagagt cacaggaaaa accggtgtgg tctttgccac
      301 aagcggaccc ggatcgacaa atctggtgac gggcatcgcc accgcatata tggacagtgt
      361 gccaatggtg gccataactg ccaatgttcc gtccaacctg gttggaagag atgcttttca
      421 ggaagtatat ataacgggaa taactctgcc tgttactaag cataattttt ttgtaaataa
      481 gattgaagat ctgccgggag cgctcaatga tgctttcaga atagcaggaa gcggcagaaa
      541 agggccggtt ctggtggata taaccaagga tgtgacggca gccacaatag attacaaaag
      601 cagaaaaatg cccgaggaac caaaaaaggt atccggcata gatgctgaag aactaaaaaa
      661 cattgccgat attataaata aatcaaaaaa accccttgta tatctgggcg gaggcgtaag
      721 agccgccgga gccggcgagg aactgggtgc ttttttaaaa aaaggagata tcccggctgc
      781 tcatacaata atggccgcag gagttcttga tgccgatgat cctcataatt cggggcttat
      841 aggcatgcat gggaccatag cctccaacag ggctgtggat aatgcggatc ttcttttggc
      901 cattgggacc agattcagtg acagagttgc attgaacccc aagaaatttg caaagaaagc
      961 caccagggta caaatagata tagataaaag tgaaatcaac aaaaatgttc tggtagacat
     1021 aggcatggtt tgcgatgcaa aggacttttt tacagctttg atgcctttaa ttgaaaaaaa
     1081 ggacagatcc gagtgggtcg gcgaggtaat ggaacttaaa aaaacaacag gagatgtcaa
     1141 atgcgatgat gcggagcttc atcccagcga aataataggc accgtctgcg atatgaccga
     1201 taaggaaaca gtctatgtaa ccgatgtagg tcagcatcag atgtgggccg ctcagtatct
     1261 taagcatgac aagacatgct gcttccttac cagcggagga ttgggaacga tggggttcgg
     1321 ctatggagct tccataggag cccaggtcgg tgctccgggc aaaagagtca tacatttcac
     1381 cggagacggc tcttttcaca tgaacctcaa tgaagcatgc acagcagtga gccagcagct
     1441 tcctataata acaataataa tgaataacag tgttctggga atggtttatc agtggcagac
     1501 atcattctac ggaagccgtt tttcagctac agaacccgga agaaaaacgg atttcgtcaa
     1561 actggctgag ggcttcggac tcaaaggatt cagagcttca aacatggatg agtttacgga
     1621 tgcattcaaa aaggcactga aggaaaaagg ccctgtgtgg atagaatgca taataggcaa
     1681 aaatgaaaaa gttcttccca tgataccggc gggcgggaca gttgaagata tgataatagg
     1741 atagaaagga gatgacgatg gctttaaaaa aagaagtgat aagtattttt gtcgacaacc
     1801 agtcgggagt attggccaga gtagtcagtc tctttggcag gcggggattc aacattgatt
     1861 cccttactgt atcggcaacg aacgatccct cgatttccag gataaccata gtgttcaagg
     1921 gcgatgatct ttccctggca cagataattt cacaaacaga aaaacttgaa gttgtcagag
     1981 atatttttac ccttgaaaaa gaaggcagtc ttttcagaga actgctgctg cttaaggtga
     2041 aagccggcaa agaggagcga acaaccgtaa aagaaatagt tgatatatac agaggaaaaa
     2101 taattgatct ttcaaaggaa agcatgatta tagaaatcac gggggcgcct gaaaaaatcg
     2161 acggtctcat gaatatgcta agctgctatg aaatcgtaga agtgtgcaga acaggaataa
     2221 cggccattga aagaggatgg caggaaaagg agaaaaaaaa tgattaaaaa gtattacgat
     2281 caggattgtg acttaaaact tctggacggc aagacaattg ctgtgatcgg attcggaagc
     2341 cagggacatg cccatgctca aaaccttaaa gaaagcggat gcagtgtggt tgtaggctta
     2401 agacctgatt caaaaagtgc aaagtctgca agggatgccg gacttgatgt tatggatgtg
     2461 gaaagcgctg ctgaaaaagg cgacattgtt atgatgctgg tcccggatga aaaggctgcc
     2521 gatatttata accggcaggt ggcaccccac atgaaagagg ggaatgtgct gatgttcgct
     2581 cacgggttca atatacatta tcagctgatc aaacccgatg agtgcctgga tgttataatg
     2641 gttgccccaa aggggccggg acatacggtg agaagccagt atatggaagg caaaggagtt
     2701 cccagcctta tagcggtaca tcatgacagg tccggaaaag ccagggatta cgctcttgca
     2761 tatgccagcg gaataggtgc gggcagagcc gggatacttg aaacatcata cagagaagag
     2821 acggaaacag atcttttcgg agagcaggcc gttttgtgcg gtggagtcac ggaacttatg
     2881 aaagccggct tcgaaactct ggttgaagcg ggatatgagc cggaaatggc ttacttcgaa
     2941 tgcatacatg aaatgaaact gatcgttgat cttataaaca gcggcggatt tgagatgatg
     3001 agaaaatccg tatcgaatac agctgagtac ggggattatt atgctggaaa acagataata
     3061 acagaagatt ccagaaaagc gatgaaacag atacttgcaa ggatacaaaa cggcgagttt
     3121 gccagtgaat tcgtcagtga aatgtcatcg ggcagacagg caagattcct gactaccagg
     3181 atgaaagaaa atgagcacca gctttgcaaa accggtgcag agctgagaaa gatgatgagt
     3241 tggttgaaaa aatgaaagaa cgaagcgata aaataaccag ggggatcgag agagctccca
     3301 taagaagcct tctttatgct ttgggttata caaaagaaga gctggagagg ccgcttatag
     3361 gagtcatttc agcccacagt gaaatcgtgc ctggccatat gaatctggac aagatcacag
     3421 atgctgtaaa agcgggagta aggatggccg gaggaactcc gatagaaatg ccatccatag
     3481 gagtatgtga cggcatagcc atgggacatg aaggcatgaa atactccctt gccagcagag
     3541 agcttattgc agacagtgta gagaccatga tacaggcaca tatgatggac gggatggtgc
     3601 ttgtcccaaa ctgcgacaag ataattccgg gcatggtcat ggctgctgtc agggtcaata
     3661 tccccacggt ggtatgttcc ggagggccaa tgatggccgg gaacgttgat gacgaggaag
     3721 taagcctttc aaaaattttt gaggcagtgg gagccagaaa agcagaaatg atagatgatg
     3781 acgagcttac aagatatgag cagggagttt gtccgggatg cggttcatgc gcaggtatgt
     3841 atactgccaa tagcatgaac agtctttgtg aggccattgg tatcgctttg ccgggaaacg
     3901 gtacgatacc tgctgtccac agtgacagac ttcgtctcgc caaacatgcg ggcatggcaa
     3961 taatggacct tctcaaaaat gacataaaag caaaagatat aataaataaa aaatccatcg
     4021 gaaatgctct tgcatgtgac atggcactgg gctgttccac caacagcgtt cttcatctgc
     4081 tggccataac ccatgaagcc ggcatagaac tggatctttc cgtgtttaac gagtacagtg
     4141 caaaagtgcc aaacctttgt catctggcac cggcaggacc tcatcacatg caggatctta
     4201 acaaagcggg aggcatacag gctattttca gtgaacttga taaaaaggga ataatagaca
     4261 gagatgtcat tactgccacc ggaaaaacag tgggagaaaa cataaagggc ataaaggtaa
     4321 aagacagtaa agtaataagg ccgatagatg atccctacag tgaaacggga ggccttgctg
     4381 ttatgtgggg caatatagcc agggagggct gtgttgtaaa aagaagtgcg gtttctcctg
     4441 aaatgctggt ccacaaggga cctgccaggg tttttgacag cgaagatgaa gcaataaata
     4501 ccatatacga aggaaaaata aatgacgggg atgtggtggt cataaggtac gaaggaccta
     4561 agggaggccc gggcatgagg gaaatgctga atcccacaag cgcccttgcc ggaatgaaaa
     4621 aagacaaaac agttgcgctt ataacagatg gaagattcag cggggcttca aggggtgcgt
     4681 ccataggtca tgtctgccct gaagcagcca gcggcggaga aatagggctc ataaaagagg
     4741 gagacatcat cgagatagat atcccttcag ccaaactgaa cgcaatggtt tcggatgaag
     4801 aatttgccga gagaaaaaaa cagcagaaga tgccggcatc ttccgtaacg aagggatggc
     4861 tggtaagata caggagaaat gttgccgggg catgcaaagg tgccgttatg gaataggaga
     4921 aaggtatttt gtatagtccg ggaaagagcg aaaatttatg aaaatgaaaa tcggatgcca
     4981 cctttctgcc gccggaggct atgaaaaaat gggaagagaa gctcttttta taggagcttc
     5041 cacttttgct ttttttacca ggaatcccag aggagccaga tcaaaggaga cagacgaaaa
     5101 agatataggt gcgctccgaa agatcatgga agataaggag tttgccccac tggtggctca
     5161 tgcaccttat acattgaacc cctgttccgc tgataaaaaa gtaagagatt tcgctttttt
     5221 tgtaatggaa gacgatctta aaaaaatgca ataccttccg ggaaattatt ataacattca
     5281 tcccgggaat catcttggac agggaatcga aaagggaata gaacttactg cagagttgct
     5341 taacaaaata ataagaccgg agtacgagac tacggtactt cttgaaacta tgacaggtaa
     5401 aggtacagag atcggcagtg tgtttggcca gctcagtgat ataatagaaa gggtccggca
     5461 aaaaaacaaa gtcggtgtct gcattgacac atgtcatata agcgatgcgg gatatgacat
     5521 aaaaaattcc cttgacgaag ttattgacga gttcgaccgc actataggtc taaaatatct
     5581 taaggccatg catataaatg acagtttgaa tatcatgggc gcaaaaaaag acagacatgc
     5641 cagactgggg gaagggatcc ttggccttga caccataata aatgtagtaa atcatccgga
     5701 tctaaaggcg ctgccgttta tactggaaac acccaatgaa cttgaaggat acaaaagaga
     5761 aatagaacta ttgaaagaag catataaagg atgaagaaac taaaagaagc aaaaacaagg
     5821 aatatgacaa atgaaaaaaa tctgcaaaag gatgtctttg aaatcataga aaagatcaga
     5881 actgaaggag atgcggcagt tttgtcatat aatgaaaaat ttgacggaag caaaagaacg
     5941 gatcttcgca tatcaaaaaa agaaatagac gaagcctata cacttacaga gaaagaagac
     6001 ccctgtttta tttcggacat aaaaaaagca gcggcaaata taaaggcctt tgcccaaaag
     6061 cagatggaga catttactga tatagacgag ttctctccgg cggaaggaat aaaactggga
     6121 cacagaataa tacccttatc atcctgttgc tgctatgtac ccggaggaaa ctatcctctt
     6181 tactccacag ctctcatgct ttgtattccc gcaaaaacag caggagtaaa aagagtaacc
     6241 gcatgttctc cgcccatcaa gggaagcggg aaaatatcac caaagaccat agtagccctt
     6301 gatacagcag gagcggatga gatatatgcg ctgggagggg cacaggctgt ggcagccttt
     6361 tcttatggta cctgtcagat aaaaccggtt gatctcatag ccggcccggg aaaccggtat
     6421 gtgactgagg ccaagagaca gtgctacgga caggtgggca tagactttct tgcaggcccc
     6481 agcgaagttc ttatcattgc cgatgagaca gctgaagcgg aaatcatagc tgccgacata
     6541 cttgcccagt cggagcatga tatcatggcc aaaggtatag cgctggttac ggatgaaaga
     6601 aaaggaaaag aaataataga ggcagtaaaa agacagttaa aagaccttaa gacggcggat
     6661 attgcagaaa aagcatggaa ggataacgga gaagtgttgg tttttgatga tcttgatgaa
     6721 ataatagaat atgccaacga tcatgccccg gaacatctgg aggtatttgt ggcagagagg
     6781 gacgagaaag acctgtgtaa gaaactgaag aattacggtt ctctttttgt gggtcgtaat
     6841 gcggctgaag tttttggtga ttatgccaca ggcaccaacc atacgctgcc gaccggaaga
     6901 gctgccaggt atacaggcgg cgtctggatt ggcactttct tgaaagtatg tacacatcag
     6961 agattgaccc cggagggaat gaacgatatt tcggatctgg tctcccgtct tgcagaggga
     7021 gaaggacttt ttgcacatgc aaaagccgca aagatacgga aatagtcaga ctttttacca
     7081 aggggtttac aagccccttt tttgttgcta taatacgcac ttgtacggtg tatcgcttta
     7141 acttgataac gcaataaaca aataataaag aaaagcgaaa aaagaaaagg aaaataaaat
     7201 ggaatttgaa acagcagatc tgaaaccgga agaaatagta actcttaaac taagaggaat
     7261 ttttcggaaa cagggctata aaaagtataa aatgagcagg tttgaagaat acggacttta
     7321 tgctgccaac aaagattttc ttggcggaga taaagtcata acctttacgg atctggacgg
     7381 gagacttctg gcccttaaac cggacgttac tttaagcata ataaaaaaca ccagagccga
     7441 tctttcttcg caggaaaagc tttattacat agaaaatgtg tacagagaaa gcaaggagag
     7501 ccatactttc aaggagatac cccaggttgg cctggaattc ataggcaaag tgggaaaaga
     7561 aggtatcata gaaaccgtat gtcttgcagc agagactttg aagacagtca gcagaaatta
     7621 tgtactggag atatcacaca tggattttgc ggtggagttg cttcagagca tggatcttaa
     7681 taaagaggag tattttcaca tccttagact gataaggagc aaaaatgccg atgggatcaa
     7741 aactgcatcg gaaaaagcgg gccttacgga aaaacagaca gaaaagttgt gcaaacttac
     7801 ggaactttac ggcagtgtgc agaagacttt gaactctgcc cgcaaacttg tcataaatga
     7861 cactatggaa gatgcacttg tacagcttga aaaagtatgc agggggatat cggcatcggg
     7921 gatcaccggc aggctgcaga tagacctttc catggtaaat gatattgact attacaacgg
     7981 gatcattttc agaggatatc taaaaaacca ccggtccaat gttctggcgg gaggccagta
     8041 tgacaaagca ctgaggcttt tcggcaaaaa agcagaaggg ataggcttcg ctttgtatct
     8101 aaatgaaata agccgggaag aaaaaataaa aagacagaca tcaaaaaaca aaaaacatca
     8161 gatgctcagt atagctcttc ccaagggacg tttgggcagc agggtctaca agctccttga
     8221 acaggcagga tacgggtgcc ccggatatga cggaaagaac aggaagctgg taattgaaaa
     8281 caaacaaaaa ggtataaggt atcttttcgt gaaacctgcc gacgtggcgg tttatgtgga
     8341 gcatcatgcg gcggatctgg gcatagtagg aaaagatata ataatggaaa cagagcctga
     8401 tgtttacgag cttatggatc tgaagacagg gatatgcagc atggtaacgg cagcaccgaa
     8461 gggatataaa gaagacacag acaggacact gaaagtggca acgaaatatg taaatgtggc
     8521 aaaacagtat tatcttgaaa aaaacagaga tatagaaata atcaaactta acggcagcat
     8581 tgaactggca ccgatactgg gactttcaga cattattgtt gacattgttg agaccggcag
     8641 cacaataaag gaaaatgatc ttgagataat agaagggttc agacagatca gcgcattttt
     8701 gatcgccaac agatcgagct ttaaattctg caacaaaatg atcacagaaa tgacagaaag
     8761 attaaggggt ataataggat gagcagattc gtcagaaaag attttttggc acttatgccc
     8821 tactctcccg gtcaacagcc tgaggagaaa atgataaagc ttaacacaaa cgaaaacccc
     8881 tatcctcctt cgcccggggc agtggatgcc atcggaaaaa tcagtaccga tgagttaagg
     8941 ctttactggg atcctgaagc ttcagaggca aaaaatgcaa tagcggagca ttacgatatt
     9001 gaaagtagca gggtcatggt gacaaacgga tccgatgagg ctctggcttt tgcctttcat
     9061 gccttcggag agaaaatatg ttttcctgaa ataacataca gtttctacaa agtttttgct
     9121 atggcaacag gcagccatgc ggtcactttt cctttaaaaa aagatatgat catagacata
     9181 acagattaca agaaattctc cggaactgtt attcttgcaa atcccaatgc gcccacaggc
     9241 atagcacttt gccgtgaaca gataagagag atagttttat cagatccgga caggctggtg
     9301 atcatcgatg aggcatatgt ggatttcggg gcagaaagct gtatatccct ggtgaaagaa
     9361 tatgacaata tactggtgat acaaaccatg agcaaatcca gaagtctggc tgccatgaga
     9421 ataggatttg cactgggaag cagagatgta atagcggatc tgaataagat aaaatacagc
     9481 tttaatccat acagtatgga cccctttgcc acagcggcag caaaagcagc aatagaagat
     9541 gaagcatact tcataaagac aacagcagaa ataataagga cccgtgaaaa gacagcggca
     9601 ggattaagag atctgggtct tgaagtgatt ccctcaaaaa caaatttttt gctggtttcc
     9661 gggggcaggg agtattttga aaaactaaag gagaaaaaaa tactggtaag atactgggat
     9721 gaaaatttct taagaaactg ggtaaggata agcatcggaa cgcctggtca gatggaaaag
     9781 ctcttgaaag ctacgaagga gataatatgc gaaaaagtga aataaaaagg aaaacagccg
     9841 aaacggacat atccatatcc cttgatattg acggaaacgg caattatgat atcagaacag
     9901 actgcggatt ttttgatcat atgctggagc ttttcacaag acacggccgt tttgatgttt
     9961 ccttgaaatg taaaggcgac agccatgttg actatcacca tacggtggaa gatacgggca
    10021 tagtcatggg tagggctttc agggaggcgc tgggagacaa aaaaggcata aaaagatacg
    10081 gaagcatgat aatgcccatg gatgaagttt tgatgctttg tgctttggat atcagcggaa
    10141 gatgcttttt gaatttcggt gtcgaattca aagttatgaa ggtaacttct ggagaaacag
    10201 aagaaagacc tatgatggcc ggtcttttcg atacggaact tgtatccgta ttcctggatg
    10261 ctttttgccg ggaactgggc cttactctcc atgtgatgaa attatccgga gagaacaccc
    10321 accatataat tgaggcggtt ttcaaaggac tgggcagagc actggcatcg gcagcggaaa
    10381 tagatccgaa agcaaaaaac gaaatacctt caacaaaagg agtactttga tgatagcagt
    10441 agcagattac ggagtcggta atcttttttc cctttgtgct tctctgaaat accttggtat
    10501 tgatacagaa gtaacaaaag atccgaaaaa aataaaagaa gcggaaagga taatactgcc
    10561 gggggtagga gcctttggcg acgcaataaa aaaacttgag gaatgcggtc tttccgaagt
    10621 gataaagaaa gaagccaaaa aaggaaaacc acttttgggc atatgcctcg gtatgcagct
    10681 tctttttgaa aggagtcttg aatacggcat acacagcgga ctagggctga ttgaagggga
    10741 agtgcgatcc atagcagatg atatgcctaa ggaagcaaga tccgggttca aggtgccgca
    10801 tatcggatgg aacagacttg atattgatga aagcgaaccg cttttcaaat atataaacaa
    10861 cggagagtat gtttactatg ttcatagctt ttatgccgga aaatgcactg aaaatactat
    10921 cgcatcatcg tatcacggca taaaaataac aggtgcctgc cgcaggggca gcatctgggg
    10981 tacccagttc catcccgaaa aaagcggaag agtgggactt gatatactga aagcatttgg
    11041 gtctgtaaag tagaaatgaa aaaatgaggc catgagaata atgtttctgc aag
//
LOCUS       DAONHK010000031        10812 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_4756_length_10812_cov_4.996189, whole genome shotgun sequence.
ACCESSION   DAONHK010000031 DAONHK010000000
VERSION     DAONHK010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 10812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10812
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4756_length_10812_cov_4.996189"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..575
                     /locus_tag="PLM92_02115"
     CDS             <1..575
                     /locus_tag="PLM92_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPR23966.1"
                     /translation="EIIEAAGKAPSGKNIQNWHFAVIKGQDMKDDIAAIITEKNESIA
                     LEMEKNDKLKADRFRKFCKNFTVFFTKAAVLTVVYSTFYKPSGFLEYESIGIDPTGEL
                     VTKRNPGMQSLGAALENFTLKAIDMGYGSCWLTSANYASNEIEEYIKNKTGFEKEGFF
                     MSALMSLGIPEENQKSPSKKDIDEICTFLK"
     gene            complement(579..1172)
                     /locus_tag="PLM92_02120"
     CDS             complement(579..1172)
                     /locus_tag="PLM92_02120"
                     /inference="COORDINATES: protein motif:HMM:NF014557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HPR23967.1"
                     /translation="MKEIIIIMMTMVLVDNIVLAKALGACSLIKLPASAGRSAAMGLA
                     VTFAMIGATLFTWPLDRYWLVPFDLEYFRIFLFIPLIFLAGYLVTAVLKKAAHSYWES
                     MGCTSSLISANCAVAGIAFINTDQGFTYFEAVYCALGAGLGFTGAMLIFAGMKYKIDK
                     ANNIPEAFRGIPAYIMALAILSMVFIGFSQAAAALIS"
     gene            complement(1169..1816)
                     /locus_tag="PLM92_02125"
     CDS             complement(1169..1816)
                     /locus_tag="PLM92_02125"
                     /inference="COORDINATES: protein motif:HMM:NF014557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HPR23968.1"
                     /translation="MIDHRSFTKKILTENPVLVMAAGICPAVAVTDSAYSGLLLGCGT
                     LLVLLLSSLPVTLMKSIIPEKAACASHIIIIAAITSVIQMLFSSWFPYVTEEVGIFIP
                     LIAVNCIVIESVNGFARNNPALPTLFNGLGAGMGFLIILLLMGIIRESIGKGTLFGDP
                     VFAEDMTLLPFAVLLPGGFFLLSLLAALIKHINRMISQKQKNAKNNHIEEEEISS"
     gene            complement(1809..2111)
                     /locus_tag="PLM92_02130"
     CDS             complement(1809..2111)
                     /locus_tag="PLM92_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPR23969.1"
                     /translation="MSEKSFFDKYMVPLAVIICFFVVSAAVVFGTHKILEYAALQKVV
                     KQKERTEKIICGNSILLLKQNEFFVKNAEKAIKDAKEKEKQEKEKDKEKDKEKDND"
     gene            complement(2108..3055)
                     /locus_tag="PLM92_02135"
     CDS             complement(2108..3055)
                     /locus_tag="PLM92_02135"
                     /inference="COORDINATES: protein motif:HMM:NF015100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HPR23970.1"
                     /translation="MNDLRKIPKSDIPEPEKMMALVLIALFPTLAAGTYIFGARVIIL
                     TVICVAACILFELLFCLAAGKKIALKDSSAAVTGVILAFLLPSEFPYWMAVAGCFFAI
                     VVAKMIFGGLGQNFVNPAAGGYAFLAVCFPEYIINLSLPSVASSQGDTAAQSPLMLWA
                     LGKTEEMPTYIEMFLGFISGGAGSVCVLTIMIGGAFLIWKKVISPLMPVSFLLGVLGT
                     SILIPGEDPLFHLLAGSTFIGAFFLAGDNATTPKGRTGKIIFGICAGVLSMGIRMSGI
                     NIDGVVFAILFMNMVTPYIDIMDKRIEKAVVHRKKGDAI"
     gene            3239..3592
                     /locus_tag="PLM92_02140"
     CDS             3239..3592
                     /locus_tag="PLM92_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007060271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HPR23971.1"
                     /translation="MAKSFNLPHDHDVNTSSLLEDIPGDDAFEKASGISALLSDARRL
                     EILWLLCHVEECVINIAAIMKMTSPAICHHLRLLKQSGLIKSRKGGKEVYYSVSDPGK
                     AEALKRLIKSMLEIN"
     gene            3626..5506
                     /locus_tag="PLM92_02145"
     CDS             3626..5506
                     /locus_tag="PLM92_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013497771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HPR23972.1"
                     /translation="MSLNKSQKKIILLISAASLFFAAGEFFAFFGNDFLRHYISLTLF
                     LMAYVTAGFDTVINAVKNILHGQVFDENFLMTIATIGALALKQFDEAAAVMIFFLLGT
                     FFENFAVSRSRDSISRLMDIKPNYANLLCEDHEEKVTPYDVAPGRIIIVRPGEKIPLD
                     GIVTEGSSSVDTSALTGESIPKDVFPGDEVVNGCINRNGLLKIKVTDTYEDSTVSRIL
                     EMTEKAADRKAPYEKFITRFARYYTPFILFAALVVVFLPPLLFPNQIFTDWVYRALIF
                     IMISCPCALVVSVPLTFFGGISKAAGSGILVKGGNYLEALSKIEIAIFDKTGTLTSGD
                     FSVSYAKALHMDSFKLLELAAHAENYSNHPIALSIKKAYGKTLDTERIEDVKEVPGKG
                     MIALIDDRLLYVGNENMMNSKGFFDLPEDESCSLVHLAFEEEYLGYIAVYDTIKEDAE
                     ETIRRIKDAGIKKTVMLTGDRREVADEVAKKTGIDEVKSDLLPGGKVAVAEKMISVNR
                     AKKGGKVAFIGDGINDAPVIARADIGISMGNMGSAAAMEASDIIIMDDRLSSLYKAIL
                     ISKKTLSVAKQNVVFSLAVKGVLLVLGAFGIASMWSAVFADVGVLLIAVLNALRSMRG
                     NE"
     gene            complement(5530..6534)
                     /locus_tag="PLM92_02150"
     CDS             complement(5530..6534)
                     /locus_tag="PLM92_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPR23973.1"
                     /translation="MEHYCFGMAVIYMKMKIIKKNNKGATLVEIIVGLAVCLIFLSVA
                     FAIISPIGNIFDRSGEKADAQQIANNVMESIRTKTAKSDTLSTSGNKIIYDSGTRFFA
                     VNNEGYLVYGETANPEEARLEYGRSFYSGKTISMTVSSQGTEKLKVIIEVKNKEGNRT
                     VYSTTGILKPILIQNEEESPAFSVEGLVNVNDSSLELTYEEKREKMGDNAEIFYYIFK
                     DYFDSLNGMYEITWNSNPIAFLAASNNEIRGYKRHDPQDTSLHIIYASREMAEIYRES
                     SVYGEVSDGKVYVTRSNGEISIDYVAVKIGDYWGKCDSTGKIVYGNQAADGWSAGTRP
                     "
     gene            complement(6504..7265)
                     /locus_tag="PLM92_02155"
     CDS             complement(6504..7265)
                     /locus_tag="PLM92_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPR23974.1"
                     /translation="MDTGRHKIIKRISGNKKGEILIESIVSFIIFIIVLIGVTAFVLS
                     SVNMNKEADISNRDLENEIKAAENDTAPVTGTGTMTIVFDGNTFYENINIKKQTAITY
                     FSPKNDEKTTHQLGEETMASIAQKFAKARAGSLPQTDGAFTGERIDGVDSSDPNAGDG
                     FAFAVYTALTTEQKSFADLISWSIVNTENGYYIFFVADRHYTSLEGATGVKVYKYDMK
                     STKYQYTGAGSVDAGRVSSSGSGWSSWNTTVSEWQ"
     gene            complement(7241..7816)
                     /locus_tag="PLM92_02160"
     CDS             complement(7241..7816)
                     /locus_tag="PLM92_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPR23975.1"
                     /translation="MIKKAIKNRKGISIPIVMAVAVILLVIGAVIMSVASGNLGTSNQ
                     EKNTKQAYFVAKSSIDVIDRSIRNGDMGEHIKNMVLTDLINSGNTAVNKNWGPFTLQV
                     SFSGKGLGNFSAEDMQITFKSSSQATSGSGSNLQSATVTINEMKVSFKSLYNDRESYR
                     VTVTYSYTGYANIQNGSRTWGTETWTLVDIK"
     gene            complement(7852..10389)
                     /locus_tag="PLM92_02165"
     CDS             complement(7852..10389)
                     /locus_tag="PLM92_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPR23976.1"
                     /translation="MSGKEAIKRKHGFTLVELIVVLVILTVVASVAVPSIAGYIIKMK
                     QMARDEMAAALFKISQTAVTDIYSAGNQEELEYNGQVDASAISPALDPEELENNADNI
                     VYMSINKSSQNKESNPLIKLLDPYVVDKEALQQTILLEYNVNTGKVLSVFYSDENDIG
                     YGQGTTYNAFERSKNNLKEGKMGYWAVDSTGMPVNTGSFEDMSIGLVDYDGELRDGET
                     QEGNEINGGRNYGLLTCECVLPAPQGSAYDYEITLDPQAGTDEVIEVNTQDWASMNTS
                     LSQALSGNGIYAYQRASGQTVLVIVLDTPYSSYSIKNKFPNLDSGNLSATLEASMGGS
                     SNTATSNTVNVFYGDQSLEGPKNVYSIYSVRHLNNIRYNTRRNSEFIQKKDVVCRDWN
                     DSILAFKALFANEAKGFTGTYKGITGNDSYKIYDLTVTGTSSAGLFAKVEGEARVTGV
                     YLDYTDEYYSAYEQASEIEKEDYFIRGTKYSGGIAGENQGIIKACTVEGRIFAKGSAS
                     AAGGIAGIISFEWWTQACPNGTPSTVTHCMSAASVNCEGMGTTGTAASAGGIVGSCDE
                     GKIYYCETGTACGVSAGTQEPVSILGVPYFGARPENTEYDYSTQNIMNNVSMITGTNA
                     YDSAGGIAGKTGVNNPAGDILYCVNAAKVISDRAGGLVGELRGYHSIVKSSYNAGDVQ
                     GDNYAGGLAGYFDIATMRSCYNTGSVKAGPVTGNRDSKYAGGLVAYMQGGAKIYDSYS
                     IGLVDQTAGPIIENSTYRNGGIGRIISGDVINCAFLRRVNHDTLNHCSALSQGQMKNS
                     ANFAGMTSHSGNYEAGIFAYYYPYLDVDNENCSLGEDFQRTPWSDHI"
     gene            complement(10406..>10812)
                     /locus_tag="PLM92_02170"
     CDS             complement(10406..>10812)
                     /locus_tag="PLM92_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006566790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HPR23977.1"
                     /translation="TAAETGHLVLSTLHTQGAAQTIDRIIDTCPPGIQKQMRGQLSMV
                     LRGIITQQLLPTASGDGRIAATEILIGNDAVANLIREDKCHQLPSVMQSSKAMGMHTM
                     NGDLAMLFKKGIITKETAISASSDKKDLAYSI"
BASE COUNT         2850 a         2528 c         2123 g         3311 t
ORIGIN      
        1 aagaaataat tgaagctgcg ggaaaggccc cctcaggcaa aaacatccag aactggcact
       61 ttgcagtaat aaaagggcag gatatgaaag acgatatagc cgcaataata accgaaaaaa
      121 atgaatccat agcccttgaa atggaaaaaa atgacaagtt aaaagcagat cgcttcagga
      181 aattctgcaa aaactttact gtttttttca caaaagcagc agtacttaca gtagtctatt
      241 ccacattcta taaaccttcg ggttttttgg aatatgaatc aataggcatc gatccaaccg
      301 gggagctggt aacaaaaaga aacccgggaa tgcagagtct tggcgctgcc ctcgaaaatt
      361 tcaccttgaa ggcaatagac atgggatacg gttcatgctg gcttaccagt gcaaattatg
      421 cctccaatga gatcgaagaa tacataaaaa acaaaacagg ttttgaaaag gaaggatttt
      481 tcatgtccgc tctcatgtct cttggtatcc cggaagaaaa ccaaaaaagc ccctcaaaga
      541 aggacattga tgagatatgt acttttctca aataaaaatc atgatataag ggcggcagca
      601 gcctgcgaaa accctataaa caccatggat aaaatggcta atgccattat atatgccgga
      661 atgcctctga aggcttccgg tatattattt gccttatcta tcttgtattt catgccggca
      721 aaaatcagca ttgcgcctgt gaagcccagc cctgcaccaa gggcacagta aacagcctca
      781 aaatatgtga acccctgatc tgtatttatg aaagctattc ccgcaacagc acaattggca
      841 gatatcaagg agcttgtgca tcccatgctt tcccaatatg aatgtgctgc ttttttcaaa
      901 actgcagtca ccagatatcc tgcaagaaat ataagcggaa tgaacaggaa tatacggaaa
      961 tactccaaat caaagggaac cagccagtac ctgtcaagag gccatgtaaa cagagtggcc
     1021 cctatcattg caaatgtaac tgccaggccc attgccgccg atcttcccgc acttgccggg
     1081 agtttgatca gagaacaggc gcccagtgct ttggctaata ctatattgtc caccaggacc
     1141 attgtcatca ttatgataat tatttctttc atgatgatat ttcttcctct tctatgtgat
     1201 tatttttggc atttttttgt ttttgcgaaa tcattctgtt tatatgttta atcaacgccg
     1261 ccagcagtga aagcaggaaa aaccctccgg gcaaaagaac agcaaagggc agcagagtca
     1321 tatcttcagc aaatacagga tcaccaaaca aagttccctt tccaatggat tcccttatga
     1381 tacccattaa aagaaggata atcagaaatc ccattcctgc tccaagaccg ttaaaaagtg
     1441 tcggcaaagc aggattgttt cttgcaaatc cgttgacact ttctataact atgcagttta
     1501 cagcaataag gggaatgaat atgccgactt cttctgttac atatgggaac catgaagaaa
     1561 aaagcatctg tatcacggaa gttattgcag ctattatgat aatatgactg gcgcaggcag
     1621 ctttttcggg tattatactt ttcatcagag ttaccggcag cgaagacaaa aggagcacta
     1681 aaagagttcc gcagcccaaa aggagtccgg aataagcgct atctgtcact gccacggcgg
     1741 gacatattcc cgccgccata acaagaacag ggttttccgt cagtattttt tttgtaaagg
     1801 atctgtgatc aatcattatc tttttccttg tctttttctt tgtctttttc cttttcctgc
     1861 ttttcttttt cttttgcatc ttttatggcc ttttcggcat tttttacgaa aaactcattt
     1921 tgtttcaata acaatatgga gtttccgcat attatttttt ctgttctttc cttctgcttt
     1981 actacttttt gcagagcagc atactcaagg atcttgtgag taccgaaaac aactgccgca
     2041 gaaaccacga aaaaacatat tataactgcc aggggcacca tatatttatc aaaaaaactc
     2101 ttttcactca tatggcatca cctttttttc tatgcactac tgctttctct atccttttgt
     2161 ccataatatc tatatagggg gttaccatat tcataaaaag tattgcaaat acaactccgt
     2221 ctatattaat tccgctcatt ctgatcccca tggacaaaac ccctgcacaa atgccgaata
     2281 ttatttttcc tgtcctgcct tttggtgttg ttgcgttatc tcccgcaagg aaaaacgccc
     2341 cgataaaggt actacctgca agcagatgaa acaaagggtc ttctcccggt atcagaattg
     2401 atgttcccaa aacacccaaa agaaaagaaa caggcatcag tggggaaatc acttttttcc
     2461 atatcaggaa tgctcccccg atcattatcg tcagcacaca aacgctgccg gctccgccgc
     2521 ttatgaatcc aagaaacatt tctatatatg tgggcatctc ttctgtcttt cccagtgccc
     2581 aaagcatcaa aggactttgt gcggcagtat ccccctgtga ggatgcgacg ctgggtaatg
     2641 aaagatttat aatgtattcg ggaaaacata ccgcaagaaa ggcatatccc cctgcggcag
     2701 gatttacaaa attttgtccc agacctccaa atatcatctt tgcgacaact atagcgaaaa
     2761 aacatcctgc taccgccatc cagtagggga actctgatgg taacagaaac gccagtataa
     2821 cccccgttac cgccgcactg gaatctttta gggcaatctt ctttccggcc gccaggcaaa
     2881 acaaaagttc aaacaaaatg catgcagcaa cacaaatcac cgtcagtatg atcactcttg
     2941 caccaaaaat atatgttcct gcggccagag tcgggaaaag ggctatcagt accagtgcca
     3001 tcattttttc cggttcgggt atatcacttt tggggatttt tctcaaatca ttcataacgt
     3061 taatacaata tcacaaagct ctattttttt ctactgtaaa tctctcttca cttcaagtac
     3121 tgctatggaa attagagtgt tttgacttga caaacaaaag cagcttatgt atcatataat
     3181 taaatagttg tttaattgtt taattgtttt atggaataaa attcagaaag gatcagatat
     3241 ggcaaaatct tttaacctgc ctcatgatca tgatgtgaat acaagctcac tgcttgagga
     3301 tatccccggt gatgatgcct ttgagaaagc ttccggtata tcggctctgc tttccgatgc
     3361 caggcgcctt gaaatattat ggcttctttg tcatgtggaa gaatgtgtga taaacattgc
     3421 cgcaataatg aaaatgactt ctcctgcaat ctgccaccat ctcaggttac tgaaacagtc
     3481 cggacttata aaaagccgga aaggcgggaa agaagtttat tattcggttt cagatcccgg
     3541 taaggctgaa gcattgaaga gactgatcaa aagcatgctt gaaataaatt aaaatatatg
     3601 ggtgttccca tgtaaggagt tctttatgtc gctgaacaaa agtcaaaaaa aaataatttt
     3661 acttatatcc gcagcttctt tgttttttgc agccggagag ttttttgctt tctttggtaa
     3721 tgatttttta cgtcattata tatctttaac actttttctg atggcatacg ttactgccgg
     3781 tttcgataca gtgatcaatg ctgtcaaaaa catattgcac ggacaggttt ttgatgagaa
     3841 tttccttatg accattgcca ccataggtgc cctggccctg aaacagtttg atgaagcggc
     3901 tgcagttatg attttcttcc ttcttggaac tttttttgag aatttcgccg tttccagatc
     3961 ccgggattcc atttcacgtc tcatggatat aaagcccaac tatgcaaatc tactttgtga
     4021 agaccatgaa gaaaaagtga ctccttatga tgtggctccc ggccggatca taattgtcag
     4081 gccgggagaa aaaatccctc tggacggaat cgtcacagaa gggtcttctt cggtggacac
     4141 ttccgccctt acaggagagt ccatcccaaa agatgttttc ccgggagatg aagttgtaaa
     4201 cggatgtata aacagaaacg gtcttctgaa aataaaagta accgatacat atgaggattc
     4261 cactgtttcc aggatactcg aaatgactga aaaagccgcc gacagaaaag ctccatacga
     4321 gaaattcatt acccgctttg caagatacta cactcccttt attttatttg cggctcttgt
     4381 tgttgtattc ctgccgcccc ttttgtttcc aaatcagatt tttacggact gggtctacag
     4441 agcacttatt ttcattatga tatcctgtcc ctgtgccctt gtggtttccg ttcctctcac
     4501 ttttttcgga ggcataagca aagcagccgg atccggcata ctggtcaagg ggggaaacta
     4561 tcttgaagct ttgtcaaaaa ttgagatcgc catttttgac aaaacgggca cgctgaccag
     4621 cggtgatttt tcagtcagct atgcaaaggc tcttcatatg gactcattca agctcctgga
     4681 actggcagcc catgctgaaa actattcgaa ccatcccata gctctttcaa taaaaaaagc
     4741 ctacggcaaa acattggata ctgaacgcat agaggatgtg aaagaggttc ccggcaaggg
     4801 catgattgca ctcatagatg acaggctttt atatgtcggc aatgaaaata tgatgaacag
     4861 caaaggcttc ttcgatctgc cggaagatga gtcatgttcc ctggtgcatt tggcttttga
     4921 agaagagtat cttggatata tagctgttta cgacaccata aaggaagatg cggaagaaac
     4981 catcagaaga ataaaggatg ccggaatcaa aaaaaccgtt atgctcaccg gggaccggcg
     5041 tgaagttgcc gatgaagtcg ccaaaaaaac cggtatagat gaagtcaaaa gcgaccttct
     5101 ccccggggga aaagttgctg tcgcagaaaa aatgatctct gtaaacagag cgaaaaaagg
     5161 aggcaaagtg gctttcatag gcgacggtat aaatgacgcc cccgtcattg ccagagccga
     5221 tataggaata tccatgggaa acatgggctc ggctgccgca atggaagctt ctgatatcat
     5281 cataatggat gacaggcttt cctccctcta taaagctatc ctgatatcaa aaaagaccct
     5341 ttccgtggca aagcaaaatg ttgttttttc cctcgctgta aaaggagtcc tgcttgttct
     5401 tggagccttc ggtatcgctt ctatgtggtc tgctgtcttt gcggatgtgg gagtgttgct
     5461 tattgctgtt ttgaatgcct tgcgttctat gagaggaaat gaataaatta aacatctgat
     5521 aattgctatt taaggccgtg tacccgccga ccagccgtca gcagcctgat taccatatac
     5581 tatctttccc gtggaatcgc atttccccca gtagtcccct atctttaccg ccacatagtc
     5641 tatacttatt tccccatttg atcttgtgac gtatactttt ccatcgctta cttcaccata
     5701 cacagaactc tccctatata tttccgccat ctcccggctt gcatatatga tatgcagtga
     5761 agtgtcctgg ggatcgtgcc ttttatatcc tcttatttca ttgtttgaag ctgccagaaa
     5821 agcaatcggg ttgctgttcc atgttatctc atacataccg ttaagagagt caaaataatc
     5881 tttgaaaata taataaaata tttctgcatt gtctcccatc ttctctcttt tttcttcgta
     5941 ggtaagttcc agagacgaat cgtttacatt aacaaggcct tccacggaaa atgcggggga
     6001 ttcctcttcg ttttgaatca atatgggttt tagtatgccg gttgttgaat aaacagttct
     6061 gttgccctct ttatttttaa cttctattat tactttcagc ttctctgttc cctgagaaga
     6121 taccgtcatt gatattgttt taccgctgta aaacgatctg ccatattcca gtctggcctc
     6181 ttcggggttc gccgtctcgc cgtagaccag atacccttca ttgttcacgg caaaaaatct
     6241 ggtaccggaa tcatatatga tcttgtttcc cgaggtggaa agagtatcac tttttgctgt
     6301 ctttgtacgg atactttcca taacattgtt tgctatctgc tgtgcatcag ccttctcccc
     6361 gctgcggtca aatatattgc ctattggcga tataatggca aatgccacac taagaaaaat
     6421 aagacatact gcaagtccca ctattatctc caccagcgtg gctcctttgt tgtttttttt
     6481 gattattttc attttcatgt atatcactgc cattccgaaa cagtagtgtt ccatgaggac
     6541 cagccgcttc ccgaagatga gacccttccg gcatcaaccg atcctgcccc cgtatattga
     6601 tatttggtac ttttcatatc atacttatac accttgaccc ccgtggcgcc ttccaggctg
     6661 gtgtaatgtc tgtctgctac gaaaaaaatg taatagccgt tttctgtatt tactattgac
     6721 cacgaaatta aatcagcaaa tgacttttgc tctgttgtaa gggcggtata aacagcaaat
     6781 gcaaaaccgt ctcccgcatt gggatcactg gaatccaccc cgtctatcct ttctcctgtg
     6841 aatgccccgt ccgtttgagg caggctccct gcccgggctt ttgcaaattt ctgtgctatt
     6901 gatgccatgg tctcttctcc cagctgatga gtggttttct catcattttt gggagaaaaa
     6961 tatgttattg cggtttgttt ttttatattt atgttttcat aaaaagtatt gccgtcaaag
     7021 actatcgtca tggttcctgt tcctgttacc ggtgccgtgt cattttctgc ggccttgatc
     7081 tcgttttcaa ggtctctgtt gctgatatcc gcctctttgt tcatgtttac ggaacttaag
     7141 acaaaggcag tcacaccaat aaggacgatt ataaatataa taaaactaac gatggattct
     7201 atgagaattt cgcccttttt gtttcctgat attcttttga ttattttatg tctaccagtg
     7261 tccatgtttc tgtcccccag gtcctagacc cgttttgaat attcgcatac cccgtatatg
     7321 aataggtgac tgtgactctg tagctttccc ggtcgttata caaagacttg aatgatacct
     7381 tcatctcatt tattgtgact gttgctgact gtagatttga tccggatccc gaagtggcct
     7441 gggaactgct cttaaaagtt atttgcatat cctctgctga aaaattaccc agccctttgc
     7501 cgctgaaact gacctgcaag gtgaacggcc cccaattttt gtttactgct gtgtttccgc
     7561 tgtttatgag atctgtcagt accatattct ttatatgttc tcccatatcc ccgttgcgta
     7621 tggacctgtc tattacatca attgatgatt tcgcaacaaa atatgcctgc ttcgtgtttt
     7681 tctcctggtt ggaagttccg agatttcccg atgccacaga cattataaca gcacctataa
     7741 caagcagtat caccgccact gccattacta tgggtatgct gatgcctttt ctgtttttta
     7801 tcgctttttt tatcatatcc tgcctttaca gcataaaagc cttataccaa atcaaatgtg
     7861 gtcgctccat ggtgttctct gaaaatcttc tcccaaactg cagttttcat tgtcaacatc
     7921 aaggtaggga taatagtatg caaatatgcc cgcctcatag tttccgctat ggcttgtcat
     7981 acctgcaaag ttcgcactgt ttttcatctg cccctgactc agggccgaac agtggtttag
     8041 agtatcatgg ttgacccttc ttagaaaagc acagtttata acatctcctg atattatcct
     8101 cccaatgcca ccgtttctgt aagtactgtt ttctattatt ggtccggctg tttgatctac
     8161 aagtcctata ctataagaat cataaatttt tgctcccccc tgcatatacg caaccagacc
     8221 tcctgcgtat tttgaatccc gattacccgt taccggtccc gcctttacag acccggtgtt
     8281 gtaacatgaa cgcatcgttg ctatgtcgaa ataacctgca agtcccccag catagttgtc
     8341 tccctgaaca tcccctgcat tgtagctgct ttttacgatc gaatggtaac cgcgaagctc
     8401 tcccacaagc cctcctgccc tgtcacttat gacttttgca gcatttacac aataaagtat
     8461 atcccctgcc gggttgttaa cgcctgtttt tcccgctatg ccaccggcag aatcatatgc
     8521 atttgttcct gttatcatgg aaacattatt cattatgttt tgtgtgctgt aatcatattc
     8581 cgtattctcg ggtcttgcgc cgaaatatgg aactccaagt atgctgaccg gctcctgcgt
     8641 tcctgcggaa acgccgcagg ccgttcctgt ttcacagtaa tatattttcc cttcatcaca
     8701 ggatcccact attccgcccg cagatgctgc ggtgccggtt gttcccattc cttcacagtt
     8761 taccgaagcc gctgacatgc aatgggtcac cgtgctggga gtcccgttgg gacatgcctg
     8821 ggtccaccac tcgaacgata tgatccctgc aatccctccg gctgcagacg cacttccctt
     8881 ggcgaatatc ctgccctcaa cagtgcatgc ctttattatt ccctgatttt ctcctgctat
     8941 gcctcccgaa tattttgtcc ctcttataaa gtaatcttct ttttctattt cagaagcctg
     9001 ctcatatgcg gaatagtact catccgtata atccaggtat actcccgtga ccctagcttc
     9061 tccttcgact tttgcaaaca gtcccgccga tgaagtgccc gtaacggtaa gatcatatat
     9121 cttgtaggaa tcattgccgg tgataccttt gtatgtccct gtgaagccct ttgcttcatt
     9181 ggcaaaaaga gctttgaagg ccaggatgga atcattccag tccctgcaaa ctacatcttt
     9241 tttctgtata aattccgaat ttcttcttgt gttatatctg atattgttca gatgacgtac
     9301 actgtatatg ctgtaaacat tttttggtcc ttccagactc tgatctccgt aaaaaacatt
     9361 tacagtgttt gacgttgccg tgtttgaact gcctcccatg gaagcttcaa gtgtggctga
     9421 aagattaccg ctgtcaagat tgggaaactt gtttttgatg ctgtaactgc tgtagggagt
     9481 atcaagtact attacaagga ctgtctgccc cgaagcccgc tggtatgcat atatcccgtt
     9541 accgctcaat gcctgggaaa gggatgtgtt catcgatgcc cagtcctgtg tattcacttc
     9601 tatcacttca tctgttcccg cctggggatc cagggttatt tcataatcat atgctgaccc
     9661 ctgaggtgcc ggcagaacac attcacaggt caaaagaccg taattcctgc ctccgtttat
     9721 ctcattgcct tcctgggtct ctccgtccct cagttctccg tcgtaatcca caagaccgat
     9781 gctcatatct tcaaaacttc ctgtgtttac aggcataccc gtactgtcca ctgcccagta
     9841 ccccattttg ccttctttga ggttgttttt gctgcgctca aaagcattat aagtggttcc
     9901 ctgtccgtat ccgatgtcat tttcgtcgct gtaaaaaaca gaaagcactt ttccggtatt
     9961 tacgttatac tccaaaagta tggtctgctg aagagcctct ttgtcaacca cataagggtc
    10021 aagcagtttg ataagcgggt ttgattcttt gttttgagag gatttattta tggacatgta
    10081 aacaatgttg tctgcattgt tctccagttc ttccggatca agagccggtg atatggccga
    10141 tgcatctacc tgtccgttat attccagctc ctcctggttg cccgccgagt aaatatcagt
    10201 tacagcagtc tggcttattt tgaaaagggc cgctgccatt tcatctcttg ccatctgctt
    10261 catctttatt atatatcctg cgatcgaagg cacggcaaca gaagcgacca ctgttaatat
    10321 gaccaggacc actattaatt ccacaagagt gaatccatgt tttcttttta tcgcttcttt
    10381 accggacatt gttttaagtt cctctctata tgctgtatgc aagatccttt ttatctgagg
    10441 aagcacttat cgctgtttct tttgttatga ttcccttttt gaaaagcatg gccagatcgc
    10501 cgttcattgt gtgcatgccc attgcttttg aagactgcat taccgaagga agctgatggc
    10561 atttatcttc ccttatgaga ttcgccactg catcgtttcc tataagtatt tctgtggcag
    10621 cgatacgccc gtcgccggaa gccgtaggca aaagctgctg ggttattatt ccccttaaca
    10681 ccattgacag ctggcctctc atttgttttt gtatacccgg agggcaggtg tctattatac
    10741 ggtcaatggt ctgcgccgct ccctgggtat gcagggtgga aagtaccaga tgtccggttt
    10801 ccgccgcagt ta
//
LOCUS       DAONHK010000032        10592 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_4863_length_10592_cov_4.280820, whole genome shotgun sequence.
ACCESSION   DAONHK010000032 DAONHK010000000
VERSION     DAONHK010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 10592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10592
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4863_length_10592_cov_4.280820"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(27..2717)
                     /gene="polA"
                     /locus_tag="PLM92_02175"
     CDS             complement(27..2717)
                     /gene="polA"
                     /locus_tag="PLM92_02175"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008679632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HPR23978.1"
                     /translation="MEKRIIIIDGNSLINRAYYAMQRPMITKEGVYTQGVYGFINMLE
                     KIKKEHPSGYIMVAFDKKAPTFRHKEYKEYKAGRKKMPPELAMQIPLLKEILSAMKIK
                     MLEKEGFEADDIIGTVAKKAEEQGLFPLIITGDKDELQLASDITKVMITKKGISQFKI
                     YDADAMKEEYGFTPFQFIDFKGLMGDQSDNIPGLPGVGEKTAKKLIYEYGSVENLLKN
                     AEQMPPGKLKDNIEDNAQLAVMSKRLASINTEMPMDIDFDEYTWQEAEKEKLISIYVK
                     LEFNSFLKRMEGKTGPHKRLQEDNREKKYKGEADLEIAEEILISSTEDVEMLDECLGN
                     AEKTVLKVFSNNDHKNPPQITGTAILVDNKYFYANYSFVEKTAQLLKKNEVTIIGHDL
                     KKDHYALMFQKTDRIKTGFDTCLSQYVLDPGRSNYDLKVLAQEYLNMEMKDEKTFMIE
                     RSQIDIFDDSSTEFSEYGKSWCWAVQKIMEEQKKRLIEENLEKVFYDVEMPLIEVMAF
                     MEYAGFMLDKSELSEIGKDVTERIEKLKELIYLQTGEEFNINSTQQLGKIIFEKLGLP
                     SGKKTKTGYSTDAEVLERLKDKHTVIPLLLEYRMLTKLKGTYIDGLLPLAAWDGRIHA
                     HFLQTVAATGRISCIEPNLQNIPIRQEMGRKIRKAFVPSSDEYILIAADYSQIELRVL
                     AHMSGDAGLIEAFNEGEDIHRTTASRVLGIPEEEINIEQRSRAKAVNFGVIYGMSAFG
                     LSSELKISRKEAEKYINDYFLKHKAVKSFMDSQVSEARENGWVKTIMGRKRYIKEINV
                     SNYTVRQIGERLAMNSPIQGSAADIIKLAMVKVHDALKKGGYRSRLILQVHDELIVEA
                     AKEEQDEVSKLLKENMESAMELKVPLFVELNAGKNWYELK"
     gene            2806..3606
                     /locus_tag="PLM92_02180"
     CDS             2806..3606
                     /locus_tag="PLM92_02180"
                     /inference="COORDINATES: protein motif:HMM:TIGR01856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HPR23979.1"
                     /translation="MKMYDYHTHTNFSEDCRVSMDDMIRAAAAKDFKEIAVTDHYDPD
                     YPNPDWTFDLDFDSYHQALLKAENDYSDHIKVIKGIEIGIQDGSTLKKCINAAGAFPY
                     DFILASFHAFDGMDICIADYEKIGHNKALYLFYSYMHKCLLKFNDYDVLGHINVIDRY
                     LGREPDYSSCMEVVETILRTIIYKGKGIEINTSSYRYGLGSRTHPTAEILKLYKTLGG
                     EILTIGSDAHFTKDIGSSFHLAKEIAEKHGFRYIACYRQRKPEMIPIF"
     gene            3728..5089
                     /locus_tag="PLM92_02185"
     CDS             3728..5089
                     /locus_tag="PLM92_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013378384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPR23980.1"
                     /translation="MTGNLLKEYLQIETIKKALSYMEKDPDKNLPKLIDWADKIDKNN
                     DFIKYRQYFRKWISDPENNWYKFMKSLWTDIDDEQRSAFFEKFILNAGFAGIKRQREL
                     REKYNCNIPWAILMDPTSACNLKCTGCWAAEYGNKLNMDLETLDGIIRQGKDLGTYMY
                     IYSGGEPLMRKDDIIKLCEKHDDCQFLAFTNATLIDEKFADEMLRVKNFFPAISVEGF
                     REETDSRRGKGTFAKVEKAMDILKKKKLAFGVSCCYTSRNTESIGSEEFIDYLIDKGA
                     KFAWYFTYMPIGNDAAVELIATADQRKFMYDRIRAFRKTKPLFTMDFWNDGEFVKGCI
                     AGGRSYLHINANGDIEPCAFIHYSDSNIKEKTLLEAYQSPLFMQYHDNQPFNDNMLMP
                     CPVLDNTGKLAEMVHACGAHSTDLKSPENVDEYCEKCRSAAEKWKPVADRIWHESHDQ
                     NNM"
     gene            complement(5169..5936)
                     /locus_tag="PLM92_02190"
     CDS             complement(5169..5936)
                     /locus_tag="PLM92_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015520407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPR23981.1"
                     /translation="MDIAVVTGASSGLGREFVRQISQKDAPDEIWAVARRKEKLEELN
                     DIADSPVRAIPLDLTKKESFDFLENLFIAENPKIKILVNAAGFGRMGKSEEIPRQEDE
                     GILDLNCRAAVSLTDAALPYMNKKSRILQICSVAAFQPLPGLNTYAASKAFLMSYTKA
                     LHYELIRSGIKVTAVCPFWIRETEFIAGIKKTDERAVRHFPFYSKPPFVVRWALRDSR
                     FNLWVSTPGPVATIMRIFSKITPHFIIVPLWDIMRRI"
     gene            complement(6150..6572)
                     /locus_tag="PLM92_02195"
     CDS             complement(6150..6572)
                     /locus_tag="PLM92_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018183246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3830 family protein"
                     /protein_id="HPR23982.1"
                     /translation="MTSIKIKSGDFLFIGDLLDKEAPMTCKVFREMLPIKSKFIHVRW
                     SGEGIWIPYGEMRTGLCYENHISHPAKGEFLFYPGGISEMEMILSYGRCCFASQYGQL
                     AGNHFMTIKEGAERLEEFGKKILWEGGQEILIEESDDE"
     gene            6765..7649
                     /locus_tag="PLM92_02200"
     CDS             6765..7649
                     /locus_tag="PLM92_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018594962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPR23983.1"
                     /translation="MHEHIMAVQRMQDHIEQHYSERITPAELSRVSLFSPWYSYRLFK
                     KYTGYTPADYIRRMRLSKSALRLRDENCRIIDVAYEMGFGSVDGYQRAFFREFGCNPG
                     SYAAEPVPIHLFTPYGVKFNELRKEKTDMENTKNVFIQIIEKPKRKVLIKRGTAAEDY
                     FDYCEEVGCELWGLLTSMRSLCGEPVSMWLPDEYKPAGTSKYVQGVEVSEDYEDNVPE
                     GFDAITLPECKYLMFQGEPFLEEKYCEAINEIRSAMDSYDPSTIGLIWDDSNPRIQLE
                     PVGERGYIEMRAVKPKVV"
     gene            complement(7683..8465)
                     /locus_tag="PLM92_02205"
     CDS             complement(7683..8465)
                     /locus_tag="PLM92_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015529946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HPR23984.1"
                     /translation="MTAEVIRGILIPFIGTSFGAACVFFMRRSIDLKLQKALTGFAAG
                     VMVAASVWSLLIPSIEQSKHMGGFSFLPAVVGFWCGILFLLLLDHLIPHFHVTGNESE
                     GPKSKLTKKTKLILAVTIHNIPEGMAVGVVYAGLISGQTEITIAAAMALSVGIAIQNF
                     PEGTVVSMPLRAEGMSKPKAFLMGMVSGAVEPAAALITIMAAGLIIPVLPYLLAFSAG
                     AMMFVVIEELIPEMTEGGHSNIGTVFFAVGFSVMMILDVALG"
     gene            complement(8481..9023)
                     /locus_tag="PLM92_02210"
     CDS             complement(8481..9023)
                     /locus_tag="PLM92_02210"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HPR23985.1"
                     /translation="MAKYAVIVVDMLNDFVTGALGCERGRDIVPHLEKLIKAAREKNI
                     PVIYSNDCHIKGIDHELELWGDHAIKGTKGAEVIPELEPEEGDYVVPKRRYSGFYQTD
                     MTLLLKELGVDTLVITGLHAHMCCRHTAADAYYLGYNIVVPGETTDSFTKDDYESGLK
                     YLEEVYGAKICSLDELISKF"
     gene            complement(9034..9582)
                     /locus_tag="PLM92_02215"
     CDS             complement(9034..9582)
                     /locus_tag="PLM92_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012424321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HPR23986.1"
                     /translation="MKNVVVYRSKYGTTKKYAEWISESLSCDIYESRQIDAGKLASYD
                     NIIYGGALYAGGVIGIDILKKNYDSICEKNIIVFTCGLGDPKEKENTNNIRQSLSKVL
                     TKEMQEGIKVFHLRGGIDYSKLNFAHRSMMSMMNRMLKKKDPEKLNDEEKQMLDTYGG
                     KVDFTDKNSIQPIIDHIKELDI"
     gene            complement(9695..>10592)
                     /locus_tag="PLM92_02220"
     CDS             complement(9695..>10592)
                     /locus_tag="PLM92_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014355446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HPR23987.1"
                     /translation="TTKEGIKVISINLLLENDTDPVIWRGPLVAKTVKQFWTDVIWGD
                     IDYMFIDMPPGTGDVPLTVFQSMALDGIIIITSPQQLVSMIVSKAVKMAEKMKIPVVG
                     IIENMSYFKCPDNGKEYRVFGESHIDEIAEKHDLKVLAKIPIEPKISVNYDKGTVELM
                     EFNWLDQVIKLLEKTEEKEMMKIAVASEKEMVTEHFGHCINFNIFEAENGVITKAKSV
                     GNPGHRPGFLPNFLNDMGVNVVISGGMGGGAIDIFNEKNIEVIVGASGDAKEAAEAYL
                     KGELKSTGSVCHEHMHHGECGE"
BASE COUNT         2941 a         2331 c         1953 g         3367 t
ORIGIN      
        1 tattcctcct gtcaatccga cctttttcat tttagttcat accagttctt tcctgcattc
       61 agttcaacaa aaagggggac ctttagttcc atggcacttt ccatattctc tttcaagagt
      121 tttgaaactt cgtcctgctc ttcttttgcg gcctccacaa taagttcatc gtgcacctgc
      181 agtatcagtc tggatctgta tcctcccttt ttgagcgcat catgaacttt taccatggcc
      241 agttttatta tatcggccgc acttccctgt atggggctgt tcatggccaa tctttctcct
      301 atctgcctta ccgtatagtt tgagacattt atttctttta tgtacctctt cctgcccatt
      361 atggttttta cccatccatt ttcccgggct tccgaaacct gactgtccat gaagcttttg
      421 accgctttat gtttcaggaa ataatcattt atatattttt ccgcttcttt tctgcttatt
      481 ttcaactctg aagaaaggcc gaaagcactc attccatata tgacaccgaa attcactgcc
      541 tttgctctgc ttctctgctc tatatttatt tcttcttccg ggatccccaa tactcttgat
      601 gctgttgttc tgtggatgtc ttcgccttca ttaaaggctt caataagccc agcatcaccg
      661 ctcatatggg caagtaccct taattctatt tgagaataat ctgctgcaat aagtatgtat
      721 tcatccgaag aaggtacaaa agctttcctt atttttcttc ccatttcctg tcttattggg
      781 atgttctgaa gattgggttc tatgcagctt atccttccgg tggccgccac agtctgcaga
      841 aaatgggcat gtatcctgcc gtcccaagct gccagaggca gaagtccgtc tatatatgtt
      901 cccttaagtt ttgtaagcat cctgtattcc agcaacagag gtattacggt atgtttatcc
      961 tttagtcttt caagtacttc agcatcagtg gaatatcctg tttttgtttt tttgcccgaa
     1021 ggcaaaccga gtttttcaaa aattatttta cccagctgct gagtcgagtt tatattgaat
     1081 tcctctcctg tctgcaaata tattagttct ttcagttttt ctatacgttc tgttacatcc
     1141 tttcctatct ccgaaagttc acttttatcc agcataaaac ccgcatattc cataaaggcc
     1201 atgacctcta taaggggcat ttccacatca tagaacacct tttcaagatt ctcttctatt
     1261 agtcgttttt tttgctcttc catgatcttt tgaactgccc agcaccatga ttttccgtat
     1321 tcactgaact ctgtggaact gtcatcaaaa atatcaattt gacttctttc tatcatgaag
     1381 gtcttttcat ccttcatctc catgttgaga tactcctgag ccagtacttt caaatcataa
     1441 ttgcttcggc ccggatccag cacatactga gacagacagg tgtcgaaacc ggtctttatt
     1501 ctatctgttt tttgaaacat caatgcataa tgatctttct tcagatcatg tcctatgatc
     1561 gtgacctcat tttttttcag aagctgcgct gttttttcaa caaaactata gtttgcataa
     1621 aaatatttat tgtcaacaag tattgctgtt cccgttattt gaggagggtt tttatgatcg
     1681 ttgttgctga atacctttaa tactgtcttt tctgcatttc caaggcattc atccagcatt
     1741 tccacatctt ccgtggagga tatgaggatt tcctctgcta tctcaagatc tgcttcccct
     1801 ttatatttct tttccctatt gtcctcctgc agccgtttat gcggtccggt tttgccttcc
     1861 atacgtttca gaaaactgtt gaattccagt ttcacatata tggatatcag tttttctttt
     1921 tctgcttcct gccatgtata ttcgtcaaaa tctatatcca tcggcatttc tgtattgatt
     1981 gaagcaagtc ttttgctcat cacagccaac tgtgcgttat cctctatatt gtccttgagc
     2041 ttgcccggcg gcatctgttc tgcatttttc agaaggtttt cgacacttcc gtattcgtat
     2101 atgagttttt ttgctgtctt ttctccgact ccgggaagtc ccggtatgtt gtcggactga
     2161 tcccccataa gtcctttgaa atctatgaac tgaaaaggtg taaaaccgta ttcttccttc
     2221 attgcatcgg catcatatat tttgaactga gatatgcctt tttttgttat cataaccttt
     2281 gtgatgtcac tggccagctg cagttcgtct ttgtctcctg ttattataag agggaaaagg
     2341 ccctgttctt ctgctttttt tgcaacagtc cctattatgt cgtctgcttc aaatccttct
     2401 ttctccagca tttttatctt catggcagaa agtatttcct tgagcaaagg tatctgcatc
     2461 gccagttcgg gcggcatttt ttttcttccg gctttgtatt ctttgtattc tttatgcctg
     2521 aatgtgggtg cttttttatc aaaggccacc attatatagc cggaaggatg ctcttttttg
     2581 attttctcca gcatatttat aaagccgtat accccttggg tatatacacc ttctttggtt
     2641 atcatgggcc tttgcatagc ataatatgct ctgtttataa ggctgtttcc gtctattatt
     2701 attatccttt tttccataaa gcacctcaaa gaaagtttaa cagatatttt tacataactc
     2761 aacaagatat gatatactgt tagacgcaaa aatgcagaaa ggcaaatgaa aatgtacgac
     2821 tatcacacgc acacaaactt ctctgaagac tgccgtgtat ccatggatga tatgatcagg
     2881 gctgcggcag caaaggactt taaggagatc gctgttacag atcactatga tcccgattat
     2941 cccaatcccg actggacttt tgatctggat tttgacagtt atcatcaagc tcttctaaaa
     3001 gcagaaaatg actatagtga ccatataaaa gtcataaagg gcatagagat aggtatacag
     3061 gacggcagca ccctgaaaaa gtgcataaat gccgcaggtg cttttcccta tgatttcatt
     3121 ttggcctctt ttcatgcctt tgacggtatg gatatatgta tcgcggatta cgaaaaaata
     3181 ggtcataata aagcacttta tcttttttat tcgtatatgc ataagtgtct tctgaagttc
     3241 aacgattatg atgttttagg ccacatcaat gtcatagaca gatacctggg ccgcgagccg
     3301 gactactcct cctgcatgga agtcgtggaa acaatcttaa ggacaatcat atacaaagga
     3361 aagggaatag aaatcaacac atcaagctac agatacggcc tcggttcccg cacccatccc
     3421 acggcggaga tacttaaatt gtataagact ctgggtggcg aaatactcac aattggatct
     3481 gatgctcact tcacaaaaga cataggatca agctttcatc tggcaaaaga aatagcagag
     3541 aaacacggtt tcaggtacat tgcctgctat cggcagagaa aaccggaaat gatcccgata
     3601 ttttaacact tatctgtttt tgttaactaa attaacattt tcttttattt gtatgaagat
     3661 atatgacctc tttcttaaga taatatatat taagataaaa aaatatataa gaaagaagga
     3721 gatgaaaatg accggtaatt tattaaagga atatcttcag atcgagacaa tcaaaaaagc
     3781 attgtcatac atggaaaagg atccggataa gaatctgcca aagcttattg actgggcaga
     3841 caaaatcgat aagaataatg attttataaa gtacagacaa tatttcagaa aatggataag
     3901 tgacccggaa aacaactggt acaagttcat gaaaagtctt tggactgaca tcgatgatga
     3961 gcagagaagt gcattcttcg agaaattcat tctgaatgca ggatttgcgg gtatcaaaag
     4021 acaaagggaa ttaagagaaa aatataactg taatatacca tgggccatcc taatggaccc
     4081 cacttctgcg tgcaacctca aatgcaccgg atgctgggct gccgaatacg gcaataaact
     4141 taatatggac ttggaaacat tggatggaat aataaggcag ggcaaggatc tgggtactta
     4201 tatgtacatc tactccggcg gggaaccgct tatgcgaaaa gatgatatta taaaactctg
     4261 tgagaagcat gatgactgtc agttcctggc cttcacaaat gccactctca ttgatgagaa
     4321 gtttgccgat gaaatgctta gggtcaagaa tttcttcccg gctataagtg tggagggttt
     4381 ccgcgaagag acagattcca gacgtggtaa aggcaccttt gcaaaagtcg aaaaggccat
     4441 ggacatatta aaaaagaaaa aactcgcctt cggagtttcc tgttgctata ccagcagaaa
     4501 taccgagtct attggcagtg aagagttcat tgactatctc attgataaag gcgccaagtt
     4561 cgcatggtac ttcacctaca tgccgatagg aaacgatgcg gccgttgaac ttattgcaac
     4621 tgcagaccaa agaaagttca tgtatgaccg gataagagca ttcaggaaga ccaagccact
     4681 ttttacaatg gacttctgga acgacggtga gtttgtaaag ggatgtattg ccggcggcag
     4741 aagctatctg cacataaatg ccaacggtga catagagcca tgtgctttca tccactattc
     4801 agattccaat ataaaagaaa aaacactgct ggaagcatat cagtctcccc tgttcatgca
     4861 gtatcacgat aatcagccct tcaatgataa tatgcttatg ccctgtcccg tacttgataa
     4921 cacaggtaaa ctggctgaga tggtacatgc ctgcggagca cactctacgg accttaagag
     4981 tccggaaaac gtggacgaat actgtgaaaa atgcagatct gcagcagaga aatggaaacc
     5041 tgtggcggat cgcatatggc atgaaagcca tgatcagaat aatatgtaat gactaaaaat
     5101 ataaaaggag ctgactgtaa aagtcagttc cttttatatt tttaatgtcc tttatactcc
     5161 tatgtccttc atatccttcg cataatatcc catagaggta caatgataaa atgaggggtt
     5221 atttttgaga atatcctcat tatcgtagcc acaggtcccg gagtcgatac ccaaagattg
     5281 aacctgctgt cacgaagggc ccatctaacc acaaaaggag gctttgaata aaaaggaaag
     5341 tgtcttactg cacgttcatc tgtctttttg atgcctgcta tgaattcggt ttcgcgtatc
     5401 caaaatgggc atacagccgt cacttttatt ccggaccgaa taagttcata atggagagcc
     5461 tttgtataac tcataaggaa tgcttttgat gcggcatatg tgttcagacc gggtagtggc
     5521 tgaaaggctg ccacagagca tatctgaagt atcctgctct tcttattcat gtatggaaga
     5581 gctgcatccg tcaatgaaac agccgcacgg caattcagat ccaggatacc ttcatcttcc
     5641 tgtcttggga tttcttcgct tttccccatt ctgccgaatc ctgccgcatt gacaagtatc
     5701 tttattttcg gattttcggc gatgaaaaga ttttccagaa aatcaaaact ttctttttta
     5761 gtcaaatcaa gagggattgc tcttacagga gaatcggcaa tgtcattcag ttcttcaagt
     5821 ttttcttttc tgcgtgccac ggcccatatt tcgtcgggtg catccttttg gcttatctgt
     5881 ctcacgaact ctcttcccag cccgcttgaa gccccggtca caactgctat atccattttc
     5941 actctctttt ctataagatc tctcctatat caggaaatac tatttataac gatctcctgc
     6001 ttctttaaga gataaaggtg tgtatccctt ttgggtcttt atatcttttc cgtctgtaat
     6061 caatcttata tccccatctt ttgacgtcat gtaaacattc ggcgtaacag agaatgccgc
     6121 atcaacagtc tcataatggg agcgattttc tattcatcat cagactcttc gatcagtatt
     6181 tcctgtccgc cttcccaaag tatttttttg ccgaactctt ccagcctttc tgctccttct
     6241 tttatagtca taaaatgatt tcccgccaat tggccgtact ggctggcgaa gcagcatctt
     6301 ccgtaggata agatcatttc catttcactt ataccaccgg gataaaagag gaattcacct
     6361 tttgccggat gtgatatgtg gttttcatag caaagtcctg tacgcatttc tccataaggt
     6421 atccatatgc cctcaccact ccacctgaca tgtatgaact tgctctttat gggaagcatt
     6481 tccctgaaga ctttgcatgt cattggagcc tccttatcaa gaaggtcgcc tatgaataaa
     6541 aagtctcccg attttatctt tatactggtc attttctgtc ctccttacaa tttatcctgt
     6601 tttttattat accatattgt tttacagcaa aaaaggtcga gaaattattt aattgtgtct
     6661 gtattatctt atcagctctt ggctttttca aacaggaata ggcaaaaata gtcgggatat
     6721 gattttgccg ctatgttaga ataaataagg agatggatac aggtatgcat gaacatataa
     6781 tggctgtaca aagaatgcag gatcatatag aacaacatta tagtgaaagg ataacacccg
     6841 ctgaactttc aagagtatca cttttttctc cctggtattc ctacagactt ttcaaaaagt
     6901 ataccggata tacgccggca gattatataa gacgcatgcg gctttcaaag tcggccttaa
     6961 gactgcgtga tgaaaactgc aggataatag acgttgccta tgaaatggga tttggcagtg
     7021 tggacggata tcagagggct ttttttcgtg aattcggatg taatcctggc agctatgctg
     7081 ccgaacctgt ccctatccat ctttttactc cctatggagt aaaattcaat gaactcagaa
     7141 aggaaaaaac tgatatggaa aatacaaaaa atgtttttat tcaaataata gaaaaaccaa
     7201 aaagaaaagt gcttatcaaa cgcggtacag ccgctgaaga ctattttgac tactgtgagg
     7261 aagtcggttg cgaattatgg gggctcttga ccagcatgag atctctttgc ggagagccgg
     7321 tttccatgtg gctacctgat gaatacaagc ctgccggaac ctcaaaatat gttcagggag
     7381 tagaagtttc cgaagactac gaagataatg tgccggaagg atttgacgca ataaccctgc
     7441 cggagtgtaa gtatctgatg tttcagggcg aaccgttttt ggaagaaaaa tactgcgaag
     7501 caatcaatga gataaggtcc gcaatggata gttatgaccc ttcgaccata ggccttatct
     7561 gggatgactc aaatcccaga atacagctgg aacctgtcgg agaaagagga tatatagaaa
     7621 tgcgtgcggt aaaaccgaaa gtagtttgat ccgctaatac aaaaacttct ccaaataatt
     7681 tttcatccga gagcgacatc aaggatcatc atgaccgaga acccgaccgc aaagaacacg
     7741 gtcccgatgt tggaatgacc gccttctgtc atttccggta taagttcttc aataacaacg
     7801 aacatcattg ccccggcaga aaatgccagc aaataaggca ggaccggtat tataagtcct
     7861 gcagccatta ttgttatcaa tgcagctgca ggctctaccg ctcctgaaac cattcccatc
     7921 aaaaaagctt ttggcttact cattccctct gccctcagag gcatagagac aactgtacct
     7981 tcaggaaagt tctgtatagc tatccccacc gaaagagcca tggctgcagc aatggttatt
     8041 tcagtttgtc ctgaaatcaa tcccgcatat accaccccga ctgccattcc ttccgggatg
     8101 ttatgtatgg tcacggcaag aataagtttt gttttttttg taagcttact ttttgggcct
     8161 tcactttcgt tgcccgtcac atggaaatga ggaatcaaat gatccaatag aagtaagaaa
     8221 agtataccgc accagaaacc aacaactgcc ggcagaaaag aaaaaccacc catgtgtttg
     8281 gactgttcaa tagaggggat caaaaggctc cagactgaag ccgccaccat tacacccgct
     8341 gcaaatccag taagtgcttt ctgcagtttg agatcgatgg atcttcgcat aaaaaaaaca
     8401 caggcagcac caaatgatgt gccgataaaa ggtatcaata ttcctcttat tacttctgct
     8461 gtcataaagt ctttcctcta tcagaacttc gaaatcaact catccagcga acatatcttt
     8521 gctccgtata cttcttcaag atatttcaga ccgctttcat agtcatcctt tgtaaatgag
     8581 tcggttgttt ctccggggac tacaatatta tatcccagat agtaagcatc tgctgcggta
     8641 tgcctgcagc acatatgggc atgaagaccc gttattacaa gagtgtccac ccccagttcc
     8701 ttgagaagaa gcgtcatatc tgtctgatag aaaccgctgt aacgcctctt ggggactaca
     8761 tagtcacctt cttcaggttc aagctccggg atcacttctg cacctttagt gcctttaatg
     8821 gcatgatcac cccaaagttc aagttcatga tctatgcctt ttatatgaca atcgtttgaa
     8881 taaataacag gtatattttt ttcacgggca gcttttatga gcttttcaag atgaggaaca
     8941 atatcccgcc ctctttcgca tccaagggct cctgtaacaa aatcgttgag catatcaaca
     9001 actatcaccg catattttgc cataactgac ctcctaaata tccaattcct ttatgtgatc
     9061 tattatgggc tgtatggaat ttttatctgt aaaatcaact tttcccccat atgtgtcaag
     9121 catctgcttt tcttcatcat tcagtttttc aggatctttc tttttcagca ttctgttcat
     9181 cattgacatc attgacctgt gggcaaaatt cagcttggag taatcgattc cccctcttaa
     9241 atgaaatacc ttgattcctt cctgcatttc ctttgtcaaa acctttgaaa ggctttgcct
     9301 tatattgttt gtgttttctt tttctttcgg atccccaagc ccgcaggtga aaacgattat
     9361 gtttttttca catatggaat catagttttt tttcaaaata tcaatgccga tcacacctcc
     9421 cgcataaagc gccccgccgt aaattatgtt atcatacgat gcaagctttc cggcatctat
     9481 ttgtctgctt tcatatatat cacatgaaag agactcgctt atccattctg cgtatttttt
     9541 tgtggtcccg tatttcgaac gataaacgac aacatttttc atggtgtgac tcctcataga
     9601 actacatcaa gctcttgata ttccgggaaa taaaaaactt tgatgtttat aattatatat
     9661 ataaacatca aagttcacaa gagaaaaaga ctttttactc tccgcattca ccatggtgca
     9721 tatgttcatg gcagacactt cctgtggatt tcaactctcc ttttagatat gcctcggctg
     9781 cttcttttgc gtccccgctg gcgcctacta tcacctcaat atttttttca ttaaaaatat
     9841 ctatagcgcc gcctcccatt ccgcctgaaa taacaacatt cactcccata tcattcaaaa
     9901 agtttggaag gaatcccggt ctgtgtcccg ggttgccaac agatttggcc tttgttataa
     9961 caccgttttc agcttcaaat atattgaagt tgatgcaatg tccaaaatgc tccgttacca
    10021 tttctttttc gcttgctact gcaattttca tcatttcttt ttcctccgtt ttttctaata
    10081 attttataac ctgatcaagc cagttgaatt ccatgagttc tactgttcct ttatcgtaat
    10141 ttaccgagat tttaggttca ataggtattt tagcgagaac tttcaaatca tgtttttcgg
    10201 ctatctcatc aatatgactt tctccaaata ccctatattc ttttccatta tccggacatt
    10261 tgaaataaga catgttttca ataataccaa caactggtat tttcattttt tcagccattt
    10321 taacggcttt tgacacaatc atagacacaa gttgttgggg agaagtaatt attattatac
    10381 catcaagagc catcgattga aacacagtaa gagggacatc accggttccc ggaggcatat
    10441 ctataaacat ataatcaata tccccccaaa ttacatctgt ccaaaattgt tttactgtct
    10501 ttgccacaag agggcctctc caaatcacgg gatctgtatc attttccagc agaagattaa
    10561 tagatattac ttttatacct tcttttgttg ta
//
LOCUS       DAONHK010000033        10445 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_4934_length_10445_cov_4.393455, whole genome shotgun sequence.
ACCESSION   DAONHK010000033 DAONHK010000000
VERSION     DAONHK010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 10445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10445
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4934_length_10445_cov_4.393455"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="PLM92_02225"
     CDS             <1..331
                     /locus_tag="PLM92_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015615142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HPR23988.1"
                     /translation="FVGSWTFIIIFIACLITWVILNTIMLVRAVDPYPYILLNLILSC
                     VAAIQAPVIMMSQNRQEEKDRLRALNDYKTNLKSEIIIEELYQKIETLIRKQDKLLEE
                     MKKTERR"
     gene            362..997
                     /locus_tag="PLM92_02230"
     CDS             362..997
                     /locus_tag="PLM92_02230"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPR23989.1"
                     /translation="MTKIHMEQYPRLAKLLTDCFFEDRLVSGQIRGIEEPEIFLEKLF
                     LMQIPVFDKTCEMNTLDDNLDSLIIGYEKKKFNQTKLILLSIFYQLKFRNELNGSDLK
                     IYKENGKAALRDLDLKWYKEFIKGNYYYIKAIAIKKGSRGKGVFRQLITPVVDYCNEN
                     SLPIILETNTSENVPVYRHFGFELVKTIKNKGGDFCQYCLIRKPETGQPDI"
     gene            1056..1238
                     /locus_tag="PLM92_02235"
                     /pseudo
     CDS             1056..1238
                     /locus_tag="PLM92_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006444920.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
     gene            1334..1861
                     /locus_tag="PLM92_02240"
     CDS             1334..1861
                     /locus_tag="PLM92_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006718376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPR23990.1"
                     /translation="MPKSRKMLSDMDAPYIRSLSELMQSQNKTTLASWSIDYAQRYIL
                     PLWEKKYPKDMRPAKALDSAREWLSGRIKLPLVKRDILECHAAASEMKGDPTSQAAAR
                     AIGQCASTVHSARHFFGLALYGTVALAYDRLGCEVPWEQIRDHAAEECGRMEASLRKV
                     AKDGENYPGRTGSRA"
     gene            1889..2149
                     /locus_tag="PLM92_02245"
     CDS             1889..2149
                     /locus_tag="PLM92_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HPR23991.1"
                     /translation="MGELSKLPNIAAKLEGQLAQVGITTIDELKNTGSREAWLRILAI
                     DPSACIMRLSALEGAIQGVRWHYLDEETKKSLKTFYNENKKQ"
     gene            2212..4089
                     /locus_tag="PLM92_02250"
     CDS             2212..4089
                     /locus_tag="PLM92_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013658911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4914 family protein"
                     /protein_id="HPR23992.1"
                     /translation="MKDYMSKLKLPGHLVEIIKAGEDVIFPETREQLIELAFGDLKND
                     LFDVEYDVEGYGIIKEATVTRCKNGAVVNYPDDYMRRRDPDCLLVADDNDTDKPKYDD
                     VYKEDFEPLRSATFEWLKKQRLIVLPFKSGGPEYGYDSLLISPANAAFFVGALADLQG
                     FISINDIADDFCPKAVIFLAPPFRHTHFEGKQIVIHNRLKEMHELFSYNLYPGPSAKK
                     GIYGILLNIGENEGWVTVHASTVKVITPYDNEIVIMHEGASGGGKSEMIEEIHRGMET
                     RIVFGQNTVTGEKNYIELKETCELRPVTDDMALCHPDIQNESGKLVVKDAEQGWFLRL
                     DHIKSYGTTPQYEKIFTQPSEPLIFLNIQGVPTATCLVWEHTLDSDGTPCPNPRVILP
                     RRLVSNVINEPVEVDVRSFGVRTPPCTRENPSYGIIGMLHILPPALAWLWRLVAPRGH
                     NNPSITTTAGMSSEGVGSYWPFATGKMVEQANLLLKQIVESSNTRYLLIPNQHIGAYE
                     VGFMPQWIAREYIARRGSAKFKPEHLNQARCPLLGYSLDSVKVDGQYIRKAFLEPEMQ
                     AEIGIEGYDKGAKILTDFFVSEAKKFDTPLLDPLGQEIIKMLLENASASEYADLMPMK
                     Y"
     gene            4151..4720
                     /locus_tag="PLM92_02255"
     CDS             4151..4720
                     /locus_tag="PLM92_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HPR23993.1"
                     /translation="MRRKDREMDRAFAEEIIDSSDFGVLSVNRDDGEVPYSVPLSLVR
                     CGENLYFHSAKEGEKVVLLARNKKARIVFVGDVNVPDIYSKTQLDEIITDKEETKKLI
                     SGVFTTEYGSAIVEGTVSKVEDKTEKEMVLKLICEKYTPDKMEYFTYALNAGGSLAQV
                     YKIKIETVTAKRKKFDTEGVEMKWGRKNG"
     gene            4713..5042
                     /locus_tag="PLM92_02260"
     CDS             4713..5042
                     /locus_tag="PLM92_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003355771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HPR23994.1"
                     /translation="MADIKDFLKLDIRVGEIVRAETFPKAKEPAYRIWVDLGEEIGIK
                     KSSAKITELYSPEDIMGKQVLCVTNFPPRQIADFMSEILVLGIYSKNGVVLIKPEVPV
                     EKGDKLG"
     gene            5184..>5856
                     /locus_tag="PLM92_02265"
     CDS             5184..>5856
                     /locus_tag="PLM92_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013150606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPR23995.1"
                     /translation="MEESKKVDLLIIGGSAGGILSANTARKVYKDISIMIIRDQQTVM
                     VPCGIPYIYGTLYDTSKNIIPDQMVTDNKIDLLIDRADSINREEKFVTTAGGKKIYYE
                     RLIIATGSLPATPETAKASFENVFSVFKDEVYLANLLKKLSNYKDVIVVGGGFIGVEF
                     SEQLKLGGKNVTLIQRGDYCLRRNFDPEFCEEAEKLLTENKVIVKKNSKIKEIIGNGK
                     AEGVIL"
     assembly_gap    5857..5866
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5867..6548
                     /locus_tag="PLM92_02270"
     CDS             <5867..6548
                     /locus_tag="PLM92_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014451159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPR23996.1"
                     /translation="ETGEKIETDAIIFGAGVRPNSKLAREAGLKIKEDDSIMVNEYMK
                     TSDPNIFAIGDCAEKVCFFTRHNVPILLASTAALEAKVAGYNAFKNKLTRTNKGTINA
                     FSTKIFGRAFGAVGLTEKQTAANDFDIVTGNFSVMDRHPGKIPGASMIKIKLIFSKYS
                     GVIMGGQISGGDSVGEMINILSIAVQQGMTAAALNTFQVATHPLLTSSPITYPINAAA
                     MDAIGKMS"
     gene            6785..7405
                     /locus_tag="PLM92_02275"
     CDS             6785..7405
                     /locus_tag="PLM92_02275"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006520535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPR23997.1"
                     /translation="MGRKENRSLFDKIAPVYGHFYNMQKKRYAEVAEKVSKEIDIASY
                     DTVIDVGCGTGALCSVLREKGLSVTGVDPAEKMLDIAIKKTEGENIRFVKGSAVGRLP
                     FENDSFDVAIASYVAHGMEKNARKKLYAEMSRLAAKKVIIYDYNKKRAFFTSLVEWME
                     GGDYFHFIRDPEDEMKNCISQMKECFSEVKVIDVDNRAAWYICTPY"
     gene            7566..8075
                     /gene="nrdG"
                     /locus_tag="PLM92_02280"
     CDS             7566..8075
                     /gene="nrdG"
                     /locus_tag="PLM92_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003529515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HPR23998.1"
                     /translation="MKDDTVRIAGKIRHSSVNGPGVRYVVFFQGCPHKCKGCQNPETH
                     DPLEGEEVYISELTDEIRNTRYIDGITISGGEPLIQPHAVKKLAEAAHLKGLDVWLYT
                     GWTYEEIMFDDTAKEVKNILKDLDVLVDGRFVDSLKSEGYLYRGSSNQRIIDVPESLD
                     RGTVIELDL"
     gene            8104..>10445
                     /locus_tag="PLM92_02285"
     CDS             8104..>10445
                     /locus_tag="PLM92_02285"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009245647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside triphosphate reductase"
                     /protein_id="HPR23999.1"
                     /translation="MEKVTKRNGAVETFDGSKIENAVAKALMATGEAGARDLSTVARA
                     VELLVETSLNDSGETLPSVERIQDEVENALMRCGYPKTAKAYILYRSQHQKIRDTKNT
                     LLDYKKLVDGYIGAQKDWRVKENSTVTLSVGGLILSNSGAITANYWLTEVYDDEIDDA
                     HSKCFMHIHDLSMLTGYCAGWSLKQLIQEGLGGVPGRITSAPANHLSTLCNQMVNFLG
                     IMQNEWAGAQAFSSFDTYLAPFVKADNLSYKETKQCIQSFVYGVNTPSRWGTQAPFTN
                     VTLDWTVPDDMKNIPAIVGGREQNFTYGDCKKEMDMINKAFIQVMIDGDAEGRGFQYP
                     IPTYSITENFDWSETENNKMLFEMTAKYGTPYFSNYINSDMNPSDVRSMCCRLRLDLR
                     ELRKKGGGFFGSGESTGSIGVVTLNLPRLAYISKTKEEFYFNLNKYMDIAARSLNVKK
                     KVITRLMNEGLYPYTKRYLGTFENHFSTIGILGMNEAIKNAKWMQGDITNKEGLLFAK
                     EVLEHMRTRLSDYQEQYGDLYNLEATPAESTTYRFAKHDVEEFGDIVTAGKEGEAPYY
                     TNSTHLPVGFTEDIFDSLELEDELQTLYTSGTVFHGFLGERLPDWKSAADLVKKIAEN
                     FRLPYYTLSPTYSICSEHGYIEGEYFTCPTCGKEAEVYSRITGYYRPVKNWNDGKTSE
                     FKERKEYEPEVYTLHDIGGRADKKIKNKDENKDTAEESKDSESENINNKKETILLSTK
                     KCPNCKKAIDVLEKADIKFNVLYAEEEEGRKLARSFHITQA"
BASE COUNT         3577 a         1813 c         2432 g         2613 t
ORIGIN      
        1 atttgtgggg agttggacat ttataataat tttcatagcc tgtctgatca cctgggtgat
       61 tttaaatact attatgcttg ttagggcggt agacccatat ccatacattc ttctcaattt
      121 gatcctttcc tgtgttgcag cgatacaggc acccgtaatt atgatgagtc agaatcgtca
      181 agaggaaaaa gacaggcttc gtgccttgaa tgactacaag acaaatttga aatccgagat
      241 cattattgaa gaattgtatc aaaaaatcga gacccttatc cgcaaacagg acaaactgct
      301 ggaggaaatg aaaaagacag agagaagata ggtcttttat aagggaagag aggattttga
      361 catgaccaag atacatatgg aacaatatcc cagactggca aagcttttga ctgactgctt
      421 ttttgaagac cggctggttt ccgggcagat aagaggaata gaagaaccgg aaatatttct
      481 tgaaaagctt tttttaatgc agatacctgt ctttgataaa acttgtgaaa tgaacacact
      541 ggatgataat ttggattcgc ttatcatagg ttatgaaaaa aagaagttca atcagacaaa
      601 attgatatta ttaagcatat tttatcaatt aaaattccga aatgaactca acggaagtga
      661 tttgaaaata tataaagaaa acgggaaagc agccttaaga gaccttgatt tgaaatggta
      721 caaagaattc ataaaaggga attactacta tatcaaagcc atagccataa aaaagggatc
      781 aagagggaaa ggcgttttcc gccaattgat aacaccggta gtggattact gcaatgaaaa
      841 ttcacttccg ataattttag aaacaaatac atctgaaaat gttcctgttt atagacattt
      901 tggatttgag cttgttaaaa cgatcaaaaa taaaggcggg gatttttgcc agtattgttt
      961 gatcaggaaa ccggagacgg ggcaaccgga tatttgatga aagggacgat attatgacat
     1021 catttgattt caaaaaagaa tacaaggatc tgtagttgcc tcccaaaaaa ccaaccgtga
     1081 tagatgtgcc gaaaatgcga tttgttatgg tggacgggca aggtgatccc aacacatctg
     1141 agtattacaa aaaggctgta gaattgcttt acggtatatc gtatggcata aagatgagca
     1201 aaaagactgg gaatcagccg gatgggtatt ttgatcatgg atgctaaaat atacgaattt
     1261 aaatcgatca tccataaagt tactgatata gatggtgcat atgtgaagct ttaataattc
     1321 ggaggtggaa agtatgccaa aaagcagaaa gatgttaagt gacatggatg ctccttatat
     1381 ccggtcccta tcagaactta tgcaaagcca aaataaaaca actcttgcaa gttggtcaat
     1441 tgattatgca cagagatata tactgcctct ttgggagaag aaatatccta aagatatgcg
     1501 tcctgcaaag gccttggact cggcaagaga atggctttcg ggcaggataa aactacccct
     1561 tgttaaaagg gatatacttg aatgccatgc tgcagccagt gaaatgaaag gcgatccgac
     1621 ctcacaggct gccgcaagag ccataggaca atgtgcatcg accgtacatt ctgcgagaca
     1681 cttttttgga cttgcacttt acggcacagt ggccttggct tatgacagac tgggatgtga
     1741 agttccctgg gagcagataa gagatcatgc agcagaagaa tgcggaagaa tggaagcatc
     1801 tttgcgaaaa gtggcgaaag atggagaaaa ttatcccggg cggacaggat caagagcata
     1861 gaaataaaaa aggaaaaaag gaaagtaaat gggagaattg tcaaaactgc cgaatattgc
     1921 ggcaaaactt gagggtcaac ttgcgcaagt tgggataacc accatcgatg aacttaaaaa
     1981 taccggaagc cgggaagcat ggctccgtat acttgcaata gatccttcgg catgtataat
     2041 gcgtttgtcg gctttggagg gcgccataca aggtgtacga tggcactatc ttgatgagga
     2101 aacaaaaaaa tctctcaaga ctttttataa tgaaaacaaa aagcaataaa tgctataata
     2161 gaaatataaa tagttttaaa ttttatattt gtgatgaatc gggaggtaaa aatgaaagac
     2221 tatatgagta aactgaaact gccgggtcac ctggtcgaaa taatcaaagc cggcgaagat
     2281 gtcatattcc ccgagacaag agagcagctg atcgagctgg cctttgggga tttaaaaaat
     2341 gacctctttg atgttgaata tgatgtagag ggatatggca taatcaagga agctactgtt
     2401 acaagatgta aaaacggtgc agtcgtaaat tatccggatg actacatgag aagacgggat
     2461 ccggactgcc ttcttgtagc agatgataat gatacagaca agcccaaata cgacgatgtt
     2521 tacaaggagg attttgaacc cttgagaagt gcgactttcg aatggctgaa aaaacaacgc
     2581 cttatcgtgc tgcccttcaa atccggaggt cctgaatacg gttatgattc acttcttatt
     2641 tcgcctgcta acgcagcttt ttttgtaggt gcccttgcgg acttacaggg gttcataagc
     2701 ataaatgata tagcagatga tttctgccca aaagctgtga tttttctggc accgcctttc
     2761 cggcataccc attttgaagg caaacagata gttatacaca acagactaaa ggaaatgcat
     2821 gaactttttt catataacct gtatcccgga cccagtgcaa aaaaaggaat ttacggaata
     2881 cttttgaata ttggtgaaaa tgaaggctgg gtaactgtac atgcttccac tgtaaaggtc
     2941 attactcctt atgataatga gatagttatc atgcacgaag gggcctccgg aggcggtaaa
     3001 agcgaaatga tagaagagat ccacagaggt atggaaacaa ggatagtttt cggtcaaaat
     3061 accgtaacgg gagagaaaaa ttatattgaa ctaaaagaaa cctgtgagct cagaccggtt
     3121 accgatgaca tggctctttg ccatcctgat atccaaaacg aaagtgggaa actcgttgtc
     3181 aaggatgcag aacagggctg gttcttgagg ctggatcata ttaaatccta cggaacgact
     3241 cctcaatatg aaaaaatatt cacacagcct tccgaacccc ttatattttt gaacatacag
     3301 ggagttccaa ccgccacttg tcttgtctgg gaacacacct tggattcgga cggaacacca
     3361 tgcccaaacc cgagagtgat cttgcccagg aggttagtgt caaatgtgat aaatgagcct
     3421 gttgaggttg atgtaagaag ttttggagta agaacacctc catgtacgag agagaatccc
     3481 tcatatggca taatcggcat gttacatata ctgccgccgg ctttggcatg gctttggaga
     3541 cttgtggcgc caagaggaca caataacccc agtataacca ccaccgccgg aatgagtagt
     3601 gaaggggtgg gatcatactg gccctttgcc acaggcaaaa tggtagaaca ggcaaatctt
     3661 ttgctgaagc aaatagtaga gtcttcaaac acaagatatc tgctcatacc gaatcagcat
     3721 ataggagcat atgaggtggg gttcatgccc caatggatag ccagagagta tatagcgaga
     3781 agaggttctg ccaaattcaa gcctgaacat ttgaaccagg ccagatgccc tttgctggga
     3841 tattcgctgg attctgtaaa ggtagatgga cagtatataa gaaaagcatt tttggaaccg
     3901 gaaatgcagg cagagatcgg catagaggga tacgataaag gtgcgaagat attgacggat
     3961 ttctttgtca gtgaagcaaa gaaatttgac actccgctcc tggaccccct tggtcaggaa
     4021 atcatcaaaa tgcttcttga aaatgcctcg gcatcggaat atgcggacct gatgccgatg
     4081 aagtattgag taaatatcgg gaaagaccaa aaagaaatca gagatatcaa agaaggggaa
     4141 gaggtgtgtg atgcgaagaa aagaccggga aatggatagg gcatttgcag aagaaatcat
     4201 agacagttca gactttggag ttttgtccgt taacagggat gatggagagg ttccatacag
     4261 tgttccgctt tcccttgtca gatgcgggga gaatctttat tttcattctg caaaggaagg
     4321 tgaaaaggtc gtattgttgg ccagaaacaa aaaggccaga atagtttttg taggggatgt
     4381 aaatgttccg gatatatatt ccaaaacgca actggatgaa atcataacag ataaggaaga
     4441 aaccaaaaaa cttatcagtg gtgtttttac taccgaatac ggatctgcca tagtcgaagg
     4501 aacagtgtca aaggtggaag acaaaactga aaaggaaatg gtcttaaaac taatatgtga
     4561 aaaatataca ccggacaaaa tggagtattt cacctatgcg cttaatgcag ggggcagcct
     4621 tgctcaggta tataagatca aaatagaaac agttactgcg aagaggaaaa aattcgatac
     4681 tgaaggtgtc gaaatgaagt ggggcagaaa gaatggctga catcaaagat tttttgaagc
     4741 tggacataag agtgggagaa atcgtaaggg ccgagacttt cccaaaggca aaagagccgg
     4801 cctacagaat atgggtggat ctgggagaag agatcggcat caaaaaatca agtgcgaaaa
     4861 tcacagaact ttacagcccg gaggatatta tgggcaaaca ggtactctgc gttacaaact
     4921 tcccgcccag acagatagcc gacttcatgt cagagatact tgttctgggc atttattcca
     4981 aaaatggagt cgtgttgata aaaccggaag ttcccgtaga aaaaggggac aaattaggat
     5041 gaatatatag aagcttaagt taaaaaatta ttgacatatg tcaaaaacaa tgatattttt
     5101 ataaagaccg atatgaatgt aaagcatgaa gtctgagaaa aacaaataac agggatttct
     5161 aattatgaaa aaaggagaaa aaaatggaag aaagcaaaaa agttgattta cttataattg
     5221 gcggaagtgc tggaggaatt ttaagcgcta acactgcaag gaaggtctac aaggatataa
     5281 gtataatgat tatccgagat cagcagaccg ttatggtgcc ttgcggtatt ccttacatct
     5341 acggaacact ttacgataca tcaaaaaaca ttattccgga tcagatggtc acggacaata
     5401 aaatcgattt attgatagac agagcagatt caataaatag ggaagaaaaa tttgttacaa
     5461 cagcaggcgg caaaaaaatc tattatgaaa ggcttataat agcgaccggt tctcttccgg
     5521 caacgcctga gactgctaaa gcctcttttg aaaatgtttt ttccgttttc aaagatgaag
     5581 tgtatcttgc caatcttctt aaaaaacttt ccaactataa agatgtaatt gtagttggag
     5641 gaggatttat aggtgttgag ttttctgagc agcttaaatt aggtggaaag aatgtgacct
     5701 tgatacaaag aggagattat tgtttaagaa gaaatttcga tcccgaattt tgtgaagagg
     5761 cagaaaaatt attaacagaa aataaagtca ttgtaaaaaa gaactctaaa ataaaagaaa
     5821 taataggtaa cggtaaagca gaaggtgtaa tactggnnnn nnnnnnggaa acaggtgaaa
     5881 aaatagaaac agatgccatc atatttggtg caggggtcag acccaattcc aaacttgcca
     5941 gggaagcagg cttgaaaata aaagaagatg attctattat ggtaaatgaa tatatgaaga
     6001 catcggatcc gaatatattt gccataggag attgtgctga aaaagtatgt ttctttacaa
     6061 gacataatgt gccgattctg ttagcttcaa ctgctgcttt ggaagcaaaa gtagccggtt
     6121 ataatgcctt taaaaacaaa ttgaccagaa caaataaagg tacaataaat gcattttcaa
     6181 ccaaaatatt cggaagagct tttggagctg ttggattgac ggagaaacaa acagcagcaa
     6241 atgattttga tatcgtgaca ggtaattttt ctgtgatgga cagacatccc ggaaaaattc
     6301 ccggagccag catgataaaa atcaaattga ttttttctaa atattcagga gttataatgg
     6361 gagggcagat ttccggcggt gattctgttg gggaaatgat caatatacta agcattgcag
     6421 ttcagcaggg gatgaccgct gcggcattga atacttttca ggtcgcaacg catccgcttc
     6481 tcacgtcttc gcccattaca tatccaataa acgcagcagc catggatgcc attggtaaga
     6541 tgtcataaaa ataaagaaga acaaaaaaat ataatatatt ttttctgacg agaggtaaaa
     6601 acttctcgtc ggtttttatt acataaaaat caattgattt agttttttat tctacagtgg
     6661 gttatagaaa cccggaataa aacttttaaa aaagaaagtt attttgtata ataactttag
     6721 aataaccaaa agccattcaa taagattttt aaagtataaa aatctttcaa ggaggtggtt
     6781 tctattgggc agaaaagaaa acagatctct ttttgataaa atagcaccgg tatacggaca
     6841 cttttataat atgcagaaaa agcgttatgc cgaggtagct gaaaaggtat caaaagaaat
     6901 agacatcgct tcatatgaca cagttatcga tgtgggttgc ggaaccggag ccctttgttc
     6961 tgttttgaga gaaaaagggc tttcagtcac aggggtggat cctgcagaga aaatgctgga
     7021 tatcgccata aagaagaccg aaggtgagaa tataaggttt gtgaaaggca gcgccgttgg
     7081 caggctgcct tttgaaaacg attcttttga tgtggccatt gcttcttatg tggctcacgg
     7141 tatggaaaaa aatgcacgca aaaagcttta tgcggaaatg agcagactgg cggcgaaaaa
     7201 ggtaataata tatgattaca ataagaaacg tgcttttttt acttctttgg tggaatggat
     7261 ggaaggcgga gactacttcc atttcataag ggatcctgaa gatgaaatga aaaactgtat
     7321 atctcaaatg aaggagtgct tctcggaagt gaaagtaata gatgtggata acagggcggc
     7381 ctggtatatt tgcactcctt attgaaaaaa gcaaattcct caacatatac gcaaaaaaac
     7441 ataaaaaacc actatatatg gtattttact gttgacattt tgatttagta agaatataat
     7501 gatttttaca ataataaata gtcttttgaa atgatcacag gcatattcac ccgggaggca
     7561 acaaaatgaa ggatgatacc gtaagaatag caggaaaaat aagacattct tctgtaaacg
     7621 gtcccggcgt gcggtacgtc gttttttttc agggatgtcc ccacaaatgc aaaggctgtc
     7681 agaatccgga gacacatgat ccgcttgaag gcgaagaagt ttatatttcg gaacttactg
     7741 atgaaataag aaatacccgg tatattgacg ggataactat ttccggagga gagcctctta
     7801 tacagcctca tgcggtaaaa aaacttgcgg aagcagcaca tttaaaggga ctggatgtct
     7861 ggctctatac aggctggaca tatgaggaaa taatgtttga cgatactgca aaagaggtaa
     7921 aaaatatttt aaaagacttg gatgtccttg tagacggcag attcgtggat tcattgaaaa
     7981 gtgagggata tctctacagg ggcagttcta atcaaaggat catagatgtg cctgaaagcc
     8041 ttgacagggg aacggttatc gaactggact tataggtatt attaaaggag agagaacatg
     8101 ggactggaaa aagtaacgaa gagaaacgga gccgtagaaa catttgacgg atccaaaata
     8161 gaaaatgctg ttgccaaggc actgatggcc accggagagg caggagccag agatctttct
     8221 actgttgcaa gagccgtcga gcttcttgtt gaaacgtctt taaatgattc gggagaaaca
     8281 ctcccttccg tagagagaat acaggacgaa gtagaaaatg ctcttatgag gtgcggatat
     8341 cccaagaccg ctaaggccta catcctttac agaagtcagc atcagaagat aagagacaca
     8401 aagaatactc ttctggatta caaaaaactg gtagacggat atataggggc acaaaaagac
     8461 tggcgtgtaa aagaaaactc aactgttact ctcagtgtgg gtggacttat tctttccaat
     8521 tccggagcaa taaccgccaa ttactggctt acagaggtat atgacgatga gatcgacgat
     8581 gctcattcaa agtgttttat gcatatacac gacctttcaa tgcttacagg ctattgtgcc
     8641 ggatggagct tgaaacagct tatccaggag gggcttggag gcgttcccgg acgtataaca
     8701 agtgcgccgg cgaatcactt gagcacactt tgcaaccaga tggtgaactt tctgggaata
     8761 atgcagaatg aatgggcggg agctcaggcg ttttcatctt ttgatacata tctggcgccc
     8821 tttgtgaagg cggacaatct aagctataaa gagacaaaac aatgcataca gtcatttgta
     8881 tacggtgtca atactccttc caggtgggga actcaggccc catttaccaa tgttactctt
     8941 gactggactg tccccgatga catgaaaaac atacctgcaa tagtaggagg ccgggagcag
     9001 aacttcactt atggagactg caaaaaagaa atggacatga taaacaaggc attcatacag
     9061 gtaatgatag acggagatgc ggaaggcagg ggtttccagt atcctatccc cacatattcg
     9121 atcaccgaaa actttgactg gagtgagacg gaaaacaaca agatgctgtt tgaaatgacg
     9181 gccaagtacg gaacacctta tttctccaat tacataaatt cagacatgaa cccctcggat
     9241 gtacgctcca tgtgctgcag actgcgcctt gatctcaggg aacttaggaa aaagggcggt
     9301 ggcttcttcg gatcgggaga aagcacgggg tccatcggcg ttgtgacgct gaaccttccc
     9361 agactggctt atatctcaaa gacaaaagaa gaattttact tcaaccttaa caaatacatg
     9421 gatatagcgg caagatctct gaatgtaaag aagaaggtaa taaccagact tatgaatgaa
     9481 ggtctctacc cttacacgaa acgctatctg gggactttcg agaatcattt ttcaactatc
     9541 ggtattttgg gcatgaatga agccataaag aatgcaaaat ggatgcaggg tgacataaca
     9601 aacaaagaag ggcttttatt tgcaaaggaa gttcttgagc atatgaggac cagactttcc
     9661 gattaccagg aacagtacgg agatctatat aatctggaag cgacacctgc cgaatcgact
     9721 acgtacaggt tcgcaaagca tgatgtggaa gagtttggtg atatagtgac tgcaggcaaa
     9781 gaaggagaag caccttatta caccaacagc acccatttgc cggtcggttt cacggaagac
     9841 atctttgaca gtcttgaact ggaggatgaa ctgcagactc tttatacttc aggaacggtt
     9901 ttccacggat tcctcggtga aagactgccc gactggaagt cggcggcgga tctggtgaag
     9961 aagatagcag agaatttcag actgccctat tacacgcttt cgcccacata ctctatttgt
    10021 tcagagcatg gttacataga aggagagtac ttcacatgtc ccacctgcgg caaagaagcg
    10081 gaagtgtatt ccaggatcac cgggtattat cgtcccgtga aaaactggaa cgacggcaaa
    10141 acatcggaat tcaaagagag aaaagaatat gagcctgaag tttatactct tcatgatata
    10201 ggtggcagag cagataaaaa gataaagaac aaagacgaaa acaaagatac cgccgaagaa
    10261 agcaaagatt ccgaaagtga aaatataaat aataaaaaag agaccattct gctttcaaca
    10321 aagaaatgtc ctaactgcaa aaaggctata gatgtgctgg aaaaagcgga tataaagttt
    10381 aatgtattat atgcagaaga ggaagaaggc aggaaactgg ccagaagttt ccatataacc
    10441 caggc
//
LOCUS       DAONHK010000034        10312 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_5011_length_10312_cov_5.000000, whole genome shotgun sequence.
ACCESSION   DAONHK010000034 DAONHK010000000
VERSION     DAONHK010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 10312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10312
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5011_length_10312_cov_5.000000"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..598)
                     /locus_tag="PLM92_02290"
     CDS             complement(<1..598)
                     /locus_tag="PLM92_02290"
                     /inference="COORDINATES: protein motif:HMM:NF024956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4131 domain-containing protein"
                     /protein_id="HPR24000.1"
                     /translation="MIVRRPLVLTATAFSLGIIFEYICSMPLAILFVSFFFVVFSVML
                     LKRKKAFLLSALFFTVFCLGGIVFFQENDYEKAANEMPAGRVCLKGEVTSAASKQKDE
                     SVLIIKPARGRRIRVSIYGKLTGIADYTGRQVVVRGELALPDQRRNPGTFDYRLYLMT
                     QGIAYLMSASPEDIQIYGHIKSPLMNKMALFRCSFEKKI"
     gene            762..3839
                     /locus_tag="PLM92_02295"
     CDS             762..3839
                     /locus_tag="PLM92_02295"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HPR24001.1"
                     /translation="MTKRNENDKSRKEIEDYLAQFDKVTDKFKEKVSDIVDEGKSPIR
                     DSGQAPTAKPAFSDSSSTGSTSKGRGKGKINSKGGRGRYKLNKKKALRLVASVIIGIC
                     FFLGFYSFTVIANAPKIDAGNIYSYLSESSVLYDDEGKAIDNVFVEEGNRINVEYEQM
                     PEDLINAAVAIEDKTFWKHHGFNVIRIFGAIKDSIFSGDSISGTSTITQQLARNVYLS
                     ETKQVRSLSRKVTEAYYSVLIEASLSKEEIVEAYLNTIYLGYNCYGVQSASQTYFSKD
                     VKDLNLLECASLAAIPRSPDTYALLKHIQKGDANAESTIKEDDILRETTDYILAYNGD
                     VSKERRDATLDFMEEQNYITASEKSKAKSKDLRSEIDPAMDSLTGLSAYFCDYTLSEV
                     VGDLMDEYNYSESEARQMLYQGGLKIYTCMDSNAQEAIEEEFKNSANFPSVVNLSKDS
                     RGNILDKWGNPLLYAYSNYIDSNDAFTLKNSEYKTEENGDMLLLKGKRLNFYKTSVGG
                     NTDYSVEFKPMYLVEDGVFYSIENGVLLIPAKYKKADKDGNLIISAQFFKDYPGFFNK
                     SGSNYIVKKSNYSMKQQVQQPQGAMVITDYKKGEVKAMAGGRNTSGRQLYNRAVSPRQ
                     PGSSIKPLSVYSSALQQGADAAEKGTPMTFNEYDKNQKTEYYGNYWTAASGINDEALV
                     VGGRVWPKNWYSGYKGMMTLRESVQQSVNVNAVRVFQQIGADYAVKQLKKFGVTTVVD
                     DESKETNDLNAAALALGGMSNGISPMEMSSAFGTFPNKGEYIEPVMYTKIENKKGEVL
                     IQNTSKKEEVIDPGVAFIMTDILRTTVTNGIAGAAATGNQPVGGKTGTTTDNYDAWFC
                     GFTPQYSAALWIGNDVNIELSTGSGAAAALWSRIMRNACEGMSGSFPGAPGNVISSST
                     SGKSEYYIRGTQDGVKSLPEEVKITVCKETGYKATPWCTETEKKKMMDDDVKAKYYCN
                     EHNIDPEKYPIDPDKKLNKDFVWIEPAKPEEDDTVTDPSVVPPEDTTTPPPAP"
     gene            3836..4465
                     /locus_tag="PLM92_02300"
     CDS             3836..4465
                     /locus_tag="PLM92_02300"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HPR24002.1"
                     /translation="MMINSNGSHIKMDLILASASPRREKMLKDAGFCPVVIPPLSEEN
                     IPMELKPEEAAMFLALKKSLSVQDTELYSRTLKSLTSRPLIVSADTIVVKNGEIIGKP
                     ADEKEALRALMKLSGCHHLVITGVAVAGTVSGLPVCRLFYEKSKVFFKKYGHKDIEEY
                     LSTKEPYDKAGGYSIQGHFRKYVDHYEGDHDNIVGLPLTSLKKVFKTFQ"
     gene            4489..5184
                     /locus_tag="PLM92_02305"
     CDS             4489..5184
                     /locus_tag="PLM92_02305"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003775566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HPR24003.1"
                     /translation="MTDISPNKDKKLIIEVDGISYERIPIKTHVVTPDDDIADVAKEY
                     AGPHIKEGDILFVSEKCTAASQKRAIPVEEIHPRKLAVTLSKYVTKTPIGIGLGMPVT
                     MEMAIQEVGTPRILLAAVAGAVGRVFRRKGWFYVVAGRKAATIDGPADYVIPPYNGCV
                     VLSPAEPDKTAKSISEKTGVEACIVDINDFNGEVMGRSSTKIDKSFIIKALKDNPLGQ
                     TRQQTPMGLIRKA"
     gene            complement(5188..5955)
                     /locus_tag="PLM92_02310"
     CDS             complement(5188..5955)
                     /locus_tag="PLM92_02310"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HPR24004.1"
                     /translation="MLFPANTMKRIFVDKSKVGDSVILIDNEDDFHHIKDVLKMRAGD
                     RILISDSKQWEYICEISGIFRNEAEARIIDKQKFASEPKLRVTLFQGIPKAGKMDLTV
                     QKAVELGVSEIVPVFMDRTVVVDRGNFWKRVVRFKSISEEASKQCGRGIIPEIRQATL
                     FSEIIQRLKSFPVIVFPYENEETTTIKDCLRRLSMNGNVPERIAIIIGPEGGFSEKEA
                     DTLKGIGAESVSLGKTILRTETAGIATLAMCMYELEL"
     gene            complement(5915..6487)
                     /gene="prmA"
                     /locus_tag="PLM92_02315"
     CDS             complement(5915..6487)
                     /gene="prmA"
                     /locus_tag="PLM92_02315"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HPR24005.1"
                     /translation="MTVKPTWEEKRGPTNGKVIYMDPGMAFGTGTHESTYLCAALIRE
                     ADCFGKKIIDAGCGSGILSIAAALMGSSRVLAVDIDPEAVRVAEENIRENNLEHIIEV
                     RKTDIMEDLAFKADIIVGNLLAEIIVKLTEKVKNDLEPGGIFIASGILTEKKEMVIKE
                     LTRQGFCIKKILEKGEWCAVSGQYNEKNFC"
     gene            complement(6524..6895)
                     /locus_tag="PLM92_02320"
     CDS             complement(6524..6895)
                     /locus_tag="PLM92_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPR24006.1"
                     /translation="MKYIEVKICTSKQGIEYVTAFLMTRGISETSIKGGEDIKELKKN
                     VDENDSEFLSSDIAEKEDEPEVTFWLEETEENRSKVMQIKIDIMMLKSDELYMELGKE
                     ADLGRLYVEDKTVSDDEWKHT"
     gene            7004..7897
                     /locus_tag="PLM92_02325"
     CDS             7004..7897
                     /locus_tag="PLM92_02325"
                     /inference="COORDINATES: protein motif:HMM:NF038196.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HPR24007.1"
                     /translation="MDTEKKIIIYYFSGTGNTEIVCGLLMQALIKRNMEVSMISMEDV
                     SFGASLDLLTPSDMIGIASPIHGFGVPKIVSQKTKQIAAMANRDQQVFLLLTGADMVK
                     INHAAASAISNIFLKKDIKIFYERLIVMASNFMVGYNERFNRQLYEVSKEKTAHMADE
                     LLLGRKRELRPSAFYRVIATATHKCESGPGASFFGNSIRSDDKCTGCMTCVKNCPVQN
                     LKITNGKIRGGKDCLMCMRCFYICPEKALTSKSFKFPILKNGYDPKNFTDPSYSAEPF
                     LTEKTKGFYRHFLKYIKNKEL"
     gene            complement(7905..8033)
                     /locus_tag="PLM92_02330"
                     /pseudo
     CDS             complement(7905..8033)
                     /locus_tag="PLM92_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014116600.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
     gene            complement(8030..9118)
                     /gene="arsB"
                     /locus_tag="PLM92_02335"
     CDS             complement(8030..9118)
                     /gene="arsB"
                     /locus_tag="PLM92_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014116598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HPR24008.1"
                     /translation="MKKKVSSEISFFEKYLSLWVLICMAAGVFIGKFLPFIPDFLGRF
                     EYANVSIPIAILIWLMIYPMMLKVDFQSVKNVGRNPKGLFVTWTVNWLIKPFTMFAIA
                     WFFFHVIFEPLITEQLARDYLAGAILLGAAPCTAMVFVWSHLTKGNAAYTVVQVATND
                     LIILAAFTPIVAFLLGVGGVSIPWDTLFLSVVLFVVIPLTGGVMTRNHITKSRGTAYF
                     NDTFIPKFGNVTIIGLLMTLVIIFSFQGDIILENPLHILLIAVPLTIQTFLIFFITYL
                     SSKAFKLPHDIAAPASMIGASNFFELAVAVAISLFGTQSPAALATIVGVLTEVPVMLI
                     LVKIANNTQRWFPENDHQRLSEKQEKVK"
     gene            complement(9137..9481)
                     /locus_tag="PLM92_02340"
     CDS             complement(9137..9481)
                     /locus_tag="PLM92_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013782342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HPR24009.1"
                     /translation="MALFGKKKEKTEKCCCGECDAASMREAEVSKKKAAGIKVLGTGC
                     AKCSRLENSVEEALKKLGIKEKVEHVTDFAVIASYGVMTTPALVIDGKVVSYGKEPDV
                     NQVVEILKKVRG"
     gene            complement(9505..10056)
                     /locus_tag="PLM92_02345"
     CDS             complement(9505..10056)
                     /locus_tag="PLM92_02345"
                     /inference="COORDINATES: protein motif:HMM:NF022321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2703 domain-containing protein"
                     /protein_id="HPR24010.1"
                     /translation="MTKKCCTCGCEEKAEKKALVEYLYLDLKSCTRCIGTDAVLDEVM
                     EMLTPVFKMAGYKVIYEKKEIETEEMANSYRFVSSPTIRVNGRDICHTVLENSCDCCS
                     DISGSDVDCRVFEYEGEKFEVPPKEMLAEAVLAGVFSQTQSGCRSSEDASTDPEYELP
                     ENLKEFFKGKKSRSCCCSGGDCC"
     gene            complement(10070..>10312)
                     /locus_tag="PLM92_02350"
     CDS             complement(10070..>10312)
                     /locus_tag="PLM92_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HPR24011.1"
                     /translation="VPIYADIFGTIPIAEALFFKGAQIGTVLSFMMAVVTLSLPSIIM
                     LRKAIKPKLLALFMGICTVGIIIVGYLFNVLQTLIV"
BASE COUNT         3064 a         2332 c         2056 g         2860 t
ORIGIN      
        1 ttattttttt ttcaaaagaa catctgaata gcgccatttt gttcatgaga gggcttttta
       61 tatggccgta tatttgaata tcctcgggtg aggcagacat caaatatgcg atcccctgtg
      121 tcataaggta cagcctgtag tcaaatgttc ccggattccg tctttgatcc ggcaaagcca
      181 actctccacg cacgacaacc tgccttcctg tatagtctgc gatccctgtc agctttccgt
      241 atatactgac tcttatcctg cgtccccgtg cgggttttat tattagaaca ctttcgtctt
      301 tttgctttga agcagcactt gtcacctctc cctttaaaca tactctgcct gcaggcattt
      361 catttgcggc tttttcataa tcgttttcct gaaaaaatac gatacctccc aaacaaaaaa
      421 ccgtaaaaaa aagtgcactc aacaaaaatg ctttttttct tttcagcaac atcaccgaaa
      481 atactacaaa aaaaaacgaa acaaacagta tggctaaagg catactgcag atatattcaa
      541 atattatacc cagggaaaac gccgttgctg tcaataccaa aggccttcta actatcattt
      601 ttgtctcctt gtgcaggcct tttttctttc cctttttcta tgatatcatt atttacatat
      661 ttttacaatc agtatgatca tcatcacaaa aaaatcttta tatgtacaca tccttatgtt
      721 ataatccact agtaaagcga caaatcaaaa gaggtgattt tttgactaaa agaaacgaaa
      781 acgacaaatc cagaaaagaa atagaagact atctggcaca gttcgataag gtgacggaca
      841 aattcaagga aaaagtctcg gacatagtcg atgaagggaa atctccgata agggattccg
      901 gacaggctcc aacggcaaag cctgcttttt ccgactcatc atccacagga tccaccagta
      961 aaggtcgcgg gaaaggtaaa ataaacagta aaggcggaag gggcagatac aagctcaaca
     1021 aaaagaaagc tttaagactg gtcgcatccg ttattattgg aatttgcttt tttcttggtt
     1081 tttattcctt cacggtcatt gccaatgcac cgaaaataga tgcaggcaac atctattctt
     1141 atctttcaga aagctctgtc ctttatgatg atgagggcaa ggccatcgac aatgtttttg
     1201 ttgaagaagg caacagaata aatgtagaat atgaacaaat gccggaagat ttgataaatg
     1261 ccgcagtagc cattgaggac aaaacattct ggaagcacca cggttttaat gtgatccgta
     1321 tattcggagc gataaaggac agtattttct caggagacag tatcagcggt accagtacca
     1381 taacccagca gctggcacgt aatgtttatc tgtcagaaac aaaacaggtc cgctctctca
     1441 gcagaaaagt aacagaggcc tattattctg ttctgataga agcttccctt tcaaaggaag
     1501 aaatagtaga agcctatctg aacaccatat atctcggata caactgttac ggagtgcaaa
     1561 gcgcttctca gacatacttt tcaaaggacg tgaaagatct gaacctgctg gaatgcgcct
     1621 ccctggcagc aatacccaga tctccggata catatgccct tttgaaacat atccagaagg
     1681 gtgatgccaa tgcggaatcc accataaagg aagatgacat cttaagggag accactgact
     1741 atatcctggc ctacaacggc gatgtttcca aggaaagaag agatgccacc ctggatttca
     1801 tggaggagca gaattatata accgcatccg aaaaaagcaa ggcaaaaagc aaagacttaa
     1861 ggtctgaaat tgatccggcc atggattctt tgaccggact ctctgcatat ttctgtgact
     1921 ataccctttc agaagtggtc ggagacctta tggatgaata taattacagc gaaagtgaag
     1981 cgcgtcagat gctttaccag ggcggactga aaatatacac ctgtatggac tcaaatgccc
     2041 aggaagccat tgaggaagaa ttcaaaaata gcgccaactt cccctccgta gtaaatctca
     2101 gcaaagactc ccgggggaat attctggaca aatggggtaa tcctctgctt tatgcataca
     2161 gtaattacat agacagcaac gatgctttta cccttaaaaa cagtgaatac aaaaccgaag
     2221 aaaacggcga tatgcttcta ttaaaaggta aaagactgaa cttctataaa acttccgttg
     2281 gcggaaatac agactacagc gttgaattca aacctatgta tcttgtagaa gacggtgttt
     2341 tctacagcat tgagaatggc gtactgctta ttccggctaa atacaaaaaa gctgataaag
     2401 atggtaacct tataataagc gcacagtttt tcaaagacta tccgggattt ttcaataaat
     2461 caggcagcaa ctatatcgtt aaaaaaagca actattccat gaagcagcag gtacagcagc
     2521 cccagggagc catggtaata acagattata aaaaaggaga agtcaaagca atggccgggg
     2581 gccgaaacac ttccggaaga cagctttaca acagggcagt ctcaccaagg caacccggtt
     2641 catcaataaa gcccctgtcg gtatattcat cggcactgca gcagggagct gatgcagcag
     2701 aaaaaggtac acctatgact ttcaacgagt acgacaagaa tcagaaaacc gaatactacg
     2761 gcaactactg gacagctgcc agcggaataa acgatgaagc cctcgttgtg ggaggccggg
     2821 tgtggcccaa aaactggtat agcggatata aaggaatgat gaccctgagg gaatcagtcc
     2881 agcagtctgt aaacgttaat gcggtaaggg ttttccagca gataggagct gactatgcgg
     2941 taaaacagct caaaaagttc ggtgtgacca cagtcgtaga tgatgaatca aaagaaacga
     3001 atgatttgaa tgcagcggct ctggccctcg gcggcatgtc aaacggcata tctcctatgg
     3061 aaatgagttc cgccttcggt actttcccca ataaaggtga atatatagaa cctgtaatgt
     3121 ataccaaaat cgaaaacaaa aaaggtgaag tacttataca aaacacatca aaaaaagaag
     3181 aagtcatcga tcccggtgtt gcctttataa tgaccgatat acttagaacc accgtaacga
     3241 acggaatcgc cggtgccgca gccacaggta atcagcctgt aggcggtaaa accggtacga
     3301 ctaccgataa ttatgatgcc tggttctgcg gtttcactcc ccagtattca gcagcactct
     3361 ggataggaaa tgacgtgaac atagagctga gtaccggttc cggtgccgcg gcagctttgt
     3421 ggagcagaat aatgcgtaat gcctgcgaag gcatgagcgg cagttttccc ggtgcaccgg
     3481 gcaatgtgat ctcttccagt accagcggaa aaagcgaata ttacataaga ggtacccagg
     3541 acggagtaaa atcacttccc gaagaagtga aaataactgt atgtaaagaa accggttaca
     3601 aggctacccc ctggtgcacc gaaacagaaa aaaagaaaat gatggatgat gatgtcaaag
     3661 caaaatacta ttgcaatgag cataacatcg atcccgaaaa atatcccata gatccggaca
     3721 agaagctcaa caaagatttc gtctggatag agccggcaaa accggaggaa gatgacacgg
     3781 taacagatcc ttccgttgtc ccgccggaag acactactac tccgcctccg gcaccatgat
     3841 gatcaatagc aatggttcac atataaaaat ggatctgata ctggcatcag catcaccaag
     3901 aagagaaaaa atgctgaagg atgcaggctt ttgtcctgtg gtcatccctc ctctttcgga
     3961 agagaatatt ccaatggaac tgaaaccgga agaggctgcc atgttcctgg cattaaagaa
     4021 gtcactgtca gtgcaggata ccgaacttta ttccaggaca ttaaaaagtc ttacatcccg
     4081 gcccttgata gtttcggcag atactatagt tgtcaaaaat ggcgagatca taggcaagcc
     4141 cgccgatgaa aaggaagccc tacgggcact aatgaaatta agcggatgcc atcacctggt
     4201 gataacagga gtggcagtgg caggcactgt ttccggtctt ccggtctgca ggctttttta
     4261 tgaaaaaagc aaggtgtttt ttaaaaaata cgggcataag gatatagaag aatatctttc
     4321 taccaaagag ccctacgata aggcgggagg atattctata cagggacatt tccgtaaata
     4381 tgttgaccat tatgagggtg atcatgacaa tatcgtcggc ctgcctctga ccagcttaaa
     4441 aaaggttttt aagaccttcc aataaatgtt ctgaaaggaa aaaacgaaat gacagatatc
     4501 tctccaaaca aagataaaaa actgattatc gaagttgacg gaatatcata cgaaaggatc
     4561 cccataaaga cccatgtggt gacgcctgac gatgatatcg ctgatgtggc aaaagagtat
     4621 gcgggccccc acataaaaga aggcgacata ctttttgtat cagagaaatg tacagcagcc
     4681 tcacaaaaaa gagctatccc cgtagaagaa atacatccaa ggaaacttgc cgtcaccctt
     4741 tctaaatatg ttaccaagac tcccataggc ataggccttg gcatgccggt cacaatggaa
     4801 atggccatac aggaagtggg aactcccagg atactgctgg ccgcagtggc tggtgcagtg
     4861 ggaagggttt ttcgcaggaa aggctggttc tatgttgttg caggacgaaa agccgccacc
     4921 atagacggcc ccgctgatta tgtaatacct ccttataacg gatgtgtggt attaagtccc
     4981 gccgagccgg acaaaacagc aaaaagtata tcagaaaaaa caggcgtaga agcctgtata
     5041 gtagatataa atgatttcaa cggtgaagtt atgggacgct ccagtactaa aatagataag
     5101 tcgtttatta tcaaagcact gaaagacaat ccccttggtc agaccagaca gcagaccccc
     5161 atgggtctta tccgaaaagc atagaagcta aagttcaagc tcatacatac acattgcaag
     5221 ggttgctata cctgccgttt cagttcgcag tatggttttt cccaaagaaa cactttcggc
     5281 gccgatacct tttagtgtat cggcttcttt ttcggaaaat ccgccctcgg gacctattat
     5341 aatggcgatc ctctcaggga catttccgtt catggaaagc cttctcagac aatcttttat
     5401 ggttgtcgtt tcttcatttt catatgggaa tacgatcacc gggaaactct tcagtctctg
     5461 tattatctcg gagaataaag tcgcctgcct tatttcgggt attattcccc tgccgcactg
     5521 cttagaagct tcttccgata tgcttttgaa ccttacgact cttttccaga aattacctct
     5581 gtctacaaca acagtacggt ccataaaaac cggaacgatt tccgatacgc ccagttccac
     5641 ggctttctgc accgtaagat ccattttgcc ggctttgggt atgccctgga acaaagtcac
     5701 tctgagtttt ggttccgaag caaatttctg tttatctata atacgtgcct cggcttcatt
     5761 tctgaagatc ccgcttattt cacagatata ttcccactgt tttgaatcgg atattaagat
     5821 gcggtctccc gcgcgcatct taagaacatc ttttatgtgg tggaaatcat cctcattatc
     5881 tattagtatg acggaatccc ccaccttgct tttgtcaaca aaaattcttt tcattgtatt
     5941 ggccggaaac agcacaccat tctccttttt ctaatatttt ttttatgcaa aatccctgtc
     6001 ttgtaagctc ttttataacc atttcttttt tttccgtcag tatgcccgat gcaataaata
     6061 tgcctccggg ctcaaggtca tttttaactt tttctgttaa cttcactatt atctctgcca
     6121 gcaggtttcc cactatgata tccgctttga atgccagatc ctccattatg tcagtctttc
     6181 tgacttctat tatatgttcc agattatttt ctctgatatt ctcttccgca actcttacag
     6241 cctccggatc aatatcaaca gcaagcactc tgctgctgcc cataagtgcc gctgcaatgg
     6301 aaagtatacc ggatccgcat cccgcatcaa tgatcttttt gccgaagcag tctgcttccc
     6361 tgatcagggc cgcacagaga taagtgcttt catgggtgcc ggttccgaat gccattcccg
     6421 gatccatgta tataactttg ccgtttgtcg gtcccctttt ttcttcccat gttggtttta
     6481 ctgttatatc atccgtaaga gatatttcgt ggaagtgttt ctttcatgta tgcttccatt
     6541 catcatcgga tacggttttg tcttctacgt aaagccttcc cagatcggct tctttaccaa
     6601 gctccatata aagttcatca cttttgagca tcatgatatc tatctttatc tgcataactt
     6661 tgctgcggtt ctcttcggtt tcctccagcc agaaagtcac ttccggttcg tcttcttttt
     6721 ctgcaatgtc ggaagacaga aattccgaat cgttttcatc aacgtttttt ttcagttctt
     6781 ttatgtcctc tccgcctttt atgctggttt cgcttatgcc ccttgtcatc agaaatgctg
     6841 tgacatattc tatgccctgt ttagatgtac atattttgac ttcaatgtat ttcattattt
     6901 tccctccgtt tttacataaa gattatatca taaaacagcg ttgtttgaaa aaaaggaagc
     6961 tatgtgctat gattttttat gcaaggtgaa aggatatttt tttatggata cagaaaagaa
     7021 aataataata tattattttt ccggcaccgg caacactgag atagtatgcg ggcttcttat
     7081 gcaggctctt attaaaagaa atatggaagt gtcaatgata tctatggaag atgtctcttt
     7141 tggtgcttct ctggatctct tgaccccttc ggatatgata ggtattgcct ctcccataca
     7201 cggttttggc gttccgaaaa tagtatcaca aaaaacaaaa caaatcgcgg caatggcaaa
     7261 ccgggatcaa caggtttttt tgctcctgac cggtgctgat atggtcaaga taaaccatgc
     7321 agcggcatct gcgatatcta atatctttct taaaaaagat ataaaaatat tctacgaacg
     7381 tcttattgtt atggcttcaa attttatggt tggttacaac gaaagattca acagacaact
     7441 atatgaggtg tccaaagaaa aaacagctca tatggctgat gaacttcttc ttggcagaaa
     7501 aagggaactt aggccttccg cattctacag agtaattgca actgctaccc ataaatgtga
     7561 aagcggccct ggcgcctcct ttttcggcaa ttcaataaga tccgatgata aatgcaccgg
     7621 ttgtatgacc tgtgttaaaa actgtcctgt gcaaaacctg aagatcacaa acggtaaaat
     7681 acgaggtggc aaagactgcc tcatgtgcat gcgctgcttt tatatttgtc ccgaaaaggc
     7741 actgacaagc aaatccttca aatttcccat actaaagaat ggttatgacc caaaaaactt
     7801 tactgatccg tcctattcag ccgagccttt cctgacagaa aaaacaaagg gattttacag
     7861 gcacttcctt aaatacatta aaaataaaga actttaggac tgcgccccat cgttactagt
     7921 acatccaccg aaggcagctc cgttatcagc ttgggatgcc ggctttcttc caaggcctcg
     7981 acgatctgac ttcggcagga attgtgtaca catataaacg cgatttttat cattttactt
     8041 tttcctgctt ttcagaaagc ctttgatggt cattttccgg gaaccagcgt tgggtgttat
     8101 ttgcaatctt tacaagtata agcattacag gcacttctgt gagtactcct actattgttg
     8161 caagggctgc cgggctttgt gtaccaaaaa gcgatatggc taccgcaacg gccagttcaa
     8221 agaaattcga cgcacctatc attgacgcgg gtgcggcaat atcatgggga agtttaaatg
     8281 ctttgctgct cagataggtt atgaagaata taaggaatgt ctgtatagtc agcggcaccg
     8341 cgatcaaaag gatatgcaga ggattttcaa gtattatatc cccctgaaaa gaaaaaatta
     8401 tgacaagggt cataagcaat cctattatgg taacatttcc gaatttgggt atgaaggtat
     8461 cgttaaaata tgccgttccc ctgctttttg tgatgtgatt tcttgtcatc actccgcctg
     8521 taagaggaat aacaacaaat aaaaccacgg ataagaaaag agtgtcccat ggaatggata
     8581 ctcctcccac acccagcaaa aaagcaacta taggggtaaa tgccgcaagt atgatcaaat
     8641 cgttcgttgc cacctggacc acggtgtaag ctgcatttcc ctttgtcaga tgactccaga
     8701 cgaagaccat tgcggtgcag ggagcagcgc ccagaagtat ggcaccggcc agataatctc
     8761 ttgcaagttg ctcagttata agcggttcaa atataacatg gaaaaaaaac caggctattg
     8821 caaacattgt aaaaggtttt atgagccagt tgaccgtcca tgtcacaaat agccctttcg
     8881 ggtttctgcc aacattcttc acactttgaa aatccacttt aagcatcatc gggtatatca
     8941 taagccatat gagtattgca atgggtattg atacattggc gtattcaaaa cgtcccagaa
     9001 aatccggtat gaatggcagg aatttgccta taaacactcc tgctgccata cagatcaaaa
     9061 cccataaact cagatacttc tcaaaaaaac ttatttcaga actcaccttt tttttcatga
     9121 aatatatctc ctctgatcag cctcttacct tttttagtat ttccacaact tggtttacgt
     9181 ccggttcctt accgtaagat accactttcc catctataac cagagcgggt gtcgtcatta
     9241 caccatagga tgctataact gcaaaatccg tcacatgctc caccttctct ttgatcccaa
     9301 gcttctttag cgcctcttcc acagaattct caagtctgct gcacttggca catccggtcc
     9361 ccagcacctt tatgcctgcc gcctttttct ttgatacttc tgcttctctc atgcttgcag
     9421 catcacattc tccgcagcaa catttttcag ttttttcctt tttctttcca aacaatgcca
     9481 tattaatatc tcctttcatc tttcttaaca gcaatctcct ccactgcagc agcacgatct
     9541 gcttttcttt cctttgaaga attcttttag gttttccgga agctcatatt ccggatccgt
     9601 acttgcatct tctgaagacc tgcatcctga ttgggtttga ctaaacactc cggcaagaac
     9661 ggcttctgca agcatttctt ttggaggcac ctcaaacttc tccccttcat attcaaaaac
     9721 cctgcagtca acatcgctgc cgcttatatc actgcagcag tcacagctgt tttcgagaac
     9781 cgtatgacag atgtccctgc cgtttaccct tatggtaggt gaggaaacaa atctatagct
     9841 gtttgccatt tcctcagttt ctatttcttt tttttcataa atgactttgt accctgccat
     9901 tttgaaaaca ggtgtgagca tttccataac ctcatccaga actgcatccg ttcctatgca
     9961 gcgggtacag cttttaagat ccaggtaaag atactcaacc agggcttttt tttcagcttt
    10021 ttcctcacac ccgcaggtgc agcatttttt tgtcatttta catttcctcc tatactatca
    10081 atgtttgcaa aacattgaat aaataaccta caataattat accgaccgtg catatgccca
    10141 taaaaagtgc cagaagtttg ggttttattg ccttacggag cattattatc gaaggcagag
    10201 aaagagtcac cactgccatc ataaacgata aaacggtgcc tatctgggct cctttgaaaa
    10261 acaatgcctc cgcaatgggt atggtaccga atatatcggc ataaatcggc ac
//
LOCUS       DAONHK010000035         9786 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_5367_length_9786_cov_4.980269, whole genome shotgun sequence.
ACCESSION   DAONHK010000035 DAONHK010000000
VERSION     DAONHK010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 9786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9786
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5367_length_9786_cov_4.980269"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..696
                     /locus_tag="PLM92_02355"
     CDS             <1..696
                     /locus_tag="PLM92_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005999772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HPR24012.1"
                     /translation="SSVARESDDVFYTWAGPEIAVASTKAYTTQLMCMYLIGLYMGYT
                     KGVIDKEYYMGMLEELKAIPDKLRLVLKNETLISDLADRFYHREQIFFIGRGPDSGVS
                     YEGSLKLKEISYINSFAIAAGELKHGTIALMEKGSLVFALSTQDTLFEKMISNIEEIK
                     ARGAYVIGITKEGNTEIEKQSDESIYIPDCPDELTPLLTVVPLQVFAYYVAKNRGCNI
                     DKPKNLAKSVTVE"
     gene            706..1956
                     /locus_tag="PLM92_02360"
     CDS             706..1956
                     /locus_tag="PLM92_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HPR24013.1"
                     /translation="MKQYEVKDINLAFSGHQKINWVKNNMPLLRGLEEEFAADRPFEG
                     IKISLSIHMEAKTAYLCKVLKAGGAALSATGSNVLSTQDDVAAALADEGINVYAIHGI
                     GEEGYKRHIEMCLSHKSNIIIDDGGDLVSMLHKERPDLASSVWGGCEETTTGIKRLRA
                     MEREGVLNFPMLAVNDADCKHLFDNRYGTGQSVWDSIMRNTNLIIAGKTVVVCGYGWC
                     GRGIAMRAAALGAGVIVTEIDPVRAIEAKMDGHQVMSMNDAAGIGDIFVTATGCSKTI
                     TLGHMKKMKNRAILANAGHFDVEVDVEAAEKTAVEKKEVRRNIMGYKMPGGKWLNIMA
                     EGRLVNIAAADGHPAEIMDLSFAVQVFSALYIKDNHKKLEKKVISVSGDIDKKIAERK
                     LESWGISIDSLTEEQRLYLNSWRI"
     gene            1965..3239
                     /locus_tag="PLM92_02365"
     CDS             1965..3239
                     /locus_tag="PLM92_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPR24014.1"
                     /translation="MDNANRVERRNKKVMRLLVVFLSIAVVVAGAFLIMGAYKNQNRI
                     EQIIAKADKLQRGYFYEKAIDVLKEDKSIYDPHDQDDPVTQKINEIKKAEEDLVKYDG
                     FVEHIFFHSLIVYPQLAFDDKGHPAIGYNAWMTTADEFKKMLPLLQERGYILYPIEEV
                     CDIDKETGKVTKKDIYLPKGKKPLIISIDDVDYYDYMKQDGFASRLVVDKSGKVATEV
                     KTPEGKTEITYDGDVMPILDRYVEKNPEFSWQGYKGIVATTGYQGVFGYRITDGLPKT
                     DEWRQEVKEISKALRESGWDIACHSYTHDGYLKDADVSLGSMKSDIDRWKSNIEPYVG
                     ETAIYISPYGFQYMSGSTAHRYIVDSGFHIFCPVSNIRKLSFYKDVMVSPRVNMDGYK
                     MMLAPDEVNEYYFDVDKVLDDARPPFDAGSLV"
     gene            3260..4447
                     /locus_tag="PLM92_02370"
     CDS             3260..4447
                     /locus_tag="PLM92_02370"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HPR24015.1"
                     /translation="MNYAKRLSWLEDQSLLKAFQLAETEDMISFSAGFPSSETYPLDA
                     VKESMARVIDNQGETALSYCSTSGYSKLRQILTKRMSDKFNLEYALDEIIITSGSQQG
                     LDMSGMLFVNEGDVVIFEEPSYLGAVNALRAYQAELVSVPTDREGMEIPALKETLEKY
                     GTRVKMVYVNPDFQNPTGRSWSDGRRKDFMEFMSGYDIPVLEDAAYGELSFDEERQKP
                     LAYYDQKGQVVYIGTFSKIFCPGLRVAWLCARRDIMEKYLVLKNAADLSSSTIAQMQM
                     ADYMENNDLDLHIKSICTLYKHRRDVMAKMIDSEFPKEVNYVLPKGGLFIWLELPEGK
                     DAAELLIRAAQKKVAFITGASFYLSTKKNNELRLNFSNMSDEKTEIGMKILGAITKEY
                     LNV"
     gene            4440..5255
                     /locus_tag="PLM92_02375"
     CDS             4440..5255
                     /locus_tag="PLM92_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPR24016.1"
                     /translation="MSKSVLTEIFNINENIKKRIKDAVSECIRDFQSSRECSTGFGEP
                     VIGYVSAKHPLFIKYLDEDICLHPKGIYRPGNTVIIHFVPFERKTAESNRGGKEPSPE
                     WTKAFYDSMHLSMKLNGIICDTLDEVGRLHSGTNIPTDWNEEKCREEWSHKLAAYAAG
                     MGRFGIAGSFHTELGFAGRLGSILVDEHYADFEERDLKMWDMERVISHIKTDSKYKGA
                     EDIRVSRKAIEACPGGAISEDGIDRKLCQAWCRKINDIIPSPEVCGKCFFYDR"
     gene            complement(5243..6904)
                     /gene="gltX"
                     /locus_tag="PLM92_02380"
     CDS             complement(5243..6904)
                     /gene="gltX"
                     /locus_tag="PLM92_02380"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HPR24017.1"
                     /translation="MDHNKLAEILFPEVERTPEYYETLYPERELPKGAMITRLGPSPT
                     GFIHLGNLYGAFIDERLAHRSGGVFYLRIEDTDEKRFVEGAVETIIDSLDFFGINFDE
                     GAGAGTGSENYGPYFQSRRKDLYSCFAKELVKKGAAYPCFMTEEEIAKTRKQQEEKKE
                     TPGIYGKYATFRDLSITEVEKHISAGKSYVLRLRSGGSGAFDGEEKNNIEINDAIRGI
                     LTMPSNYQDIVILKANGMPTYHFAHVCDDHFMRTTHVVRGEEWLSSLPIHVELFEKLN
                     FRPPIYCHTAQLMKLDENGNKRKLSKRNDPELSLDFYKEKGYHPEAVREYLLTLLNSN
                     YEEWRTQNPDIPCEEFPFSMEKMSTSGALFDLDKLNDVSKECLAGIPAASLREFLEKW
                     ASQYRPEVYAALKENEGYLESIIDIGRNDPKPRKDFICASQMFEYIKYFFDAFFEIED
                     RLPEQVDSRDAISILKAYLSTYDPCDDQSTWFSKIRDIASEFSYAARPKDYKKDPDAY
                     KGHVGHVSTVIRIAITGRSQSPDIWTIQNIMGEAKVRSRIMDYLS"
     gene            7141..8424
                     /locus_tag="PLM92_02385"
     CDS             7141..8424
                     /locus_tag="PLM92_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HPR24018.1"
                     /translation="MDSHSITAIAAIIVLVILSGYFSATETAFTSLNRIKIKNLAGEG
                     NKRAKRVLHLSERYDKLLSTILIANNVVNITMTAVATVLFINLYGDMGATISTIVITI
                     VVLIFGEISPKTMAKEAPESFSMFSAPIIHFFETLFTPVNFIFAKWKQLIIKIFKIGD
                     SSTITEDELKTMVEEAETEGGIDEDQSELIQNAIEFNELQARDVLTPRVDMEAVDIEA
                     TEEEVASKFRSTGYSRLPVYEDDLDSIMGVLNQKDFHNYIVGTEKTVSDFVKPVVFVG
                     ETMKISALLRKLQLVKTHIAIIVDEYGGTEGLVTMEDIIEELVGEIYDEHDAIMSQEV
                     TELQNGSYRVMCSASLSKVFDFFGIYDELDVNTVNGWAVLCLDKLPEKGDIFEYKSGK
                     KLLKARVTKANGKKALELNVTAEELTREEEEEVEE"
     gene            8425..>9786
                     /locus_tag="PLM92_02390"
     CDS             8425..>9786
                     /locus_tag="PLM92_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005837822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPR24019.1"
                     /translation="MGLMEKVFGDINAKEVKKLEKIADKIDLMDREMQQLTDGQLQEK
                     TPEFKKRLKEGETLDDILPEAFAVCREAAWRTLGMKHFYVQLIGGIALHQGRIAEMKT
                     GEGKTLVATLPVYLNALEEKGVHVVTVNDYLAKRDMEWMGKIYNFLGLSTGCVIHGIT
                     EEDRRAAYAADVTYGTNNEYGFDYLRDNMVIYKEEMTQRDLNYAIVDEVDSILVDEAR
                     TPLIISGQGDKSTELYNQANRFVKTLKNEKDYTIEEKDKQVSLTEEGVSRCEEYFRVD
                     NFSDPENMEINHHVLQALKAHNLMKRDVDYIVKDGEIIIVDEFTGRLMFGRRYSEGLH
                     QAIEAKEGVYVRSESKTLATITLQNYFRMYQKLAGMTGTAKTEENEFREIYNMDVVVI
                     PTNKPVIREDCQDAVFATEKGKYKAIADRVAEAHESGQPVLVGTISIEKSELISEMLQ
                     RRGV"
BASE COUNT         3150 a         1795 c         2378 g         2463 t
ORIGIN      
        1 agttctgtag caagagaatc agatgatgtc ttttatacat gggcgggtcc tgagatagca
       61 gttgcatcaa caaaagcata taccacgcag ctcatgtgca tgtatcttat cggtctttat
      121 atgggctaca caaaaggtgt tatagacaaa gaatattaca tgggaatgct tgaagaactc
      181 aaagcgatac ctgacaagtt gagacttgtc ctgaaaaacg aaactcttat atcggatctg
      241 gcagacagat tctaccacag agagcagata ttttttatcg gcagggggcc ggattcggga
      301 gtctcatatg aaggatctct caagctcaag gaaatctcat atatcaattc ttttgccata
      361 gcggcaggtg agcttaaaca cggcaccatt gcccttatgg aaaaaggctc gctggtcttt
      421 gccctttcca cacaggacac attgtttgaa aagatgatat ccaatatcga agagataaag
      481 gcaagagggg cctatgttat cgggataacc aaagaaggaa atacagaaat agaaaagcag
      541 tccgatgaaa gcatttatat acccgattgt cccgatgagc ttacaccgct gcttacggtg
      601 gtgccgcttc aggtgttcgc gtattatgtg gcaaaaaaca gaggatgcaa tatagataag
      661 ccgaaaaacc tggcgaaatc agtgactgtt gaataggaga gatcaatgaa acagtatgaa
      721 gtaaaggaca taaatctggc attttccggg caccagaaaa taaattgggt aaaaaacaat
      781 atgcctcttc ttcgcgggct tgaggaggag ttcgccgcgg acagaccctt tgaaggaata
      841 aaaatatctc tttcaataca tatggaagca aaaacagcct atttatgtaa ggtactaaag
      901 gccgggggag cagctctttc agccacaggc agcaatgttc tttcaactca ggatgatgtg
      961 gcggcagccc ttgcggatga agggataaat gtgtatgcga tacacgggat aggggaagaa
     1021 ggatacaaaa gacatataga aatgtgcctt tcccataagt caaatataat aatagatgac
     1081 ggaggagatc ttgtttccat gcttcacaag gagagacctg atctggcttc ttcggtatgg
     1141 ggcggatgtg aagaaacaac aacggggata aaaaggctta gagcaatgga gcgggagggg
     1201 gtattaaact tccccatgct ggccgtaaac gatgctgact gcaaacatct ttttgacaac
     1261 cgttacggaa cggggcagtc tgtgtgggac agcataatgc gcaataccaa ccttattatc
     1321 gcaggcaaaa cagtggtagt atgcggatac ggatggtgtg gcaggggcat tgccatgaga
     1381 gcggcagctc ttggtgccgg agtgatagtt acggagatag atccggtaag agcgattgaa
     1441 gcaaagatgg acggccatca ggtgatgagc atgaatgatg ctgccggcat cggagatatt
     1501 tttgttaccg ccaccggatg ttcaaaaacc ataacgttgg gtcatatgaa aaaaatgaaa
     1561 aacagagcaa tacttgccaa tgcaggacat tttgatgttg aggtggatgt tgaagcagcc
     1621 gaaaaaacag cggtagagaa aaaagaagtc cgcagaaata ttatgggata taaaatgccc
     1681 ggcggcaaat ggttaaatat aatggcagaa ggtaggctgg tcaatattgc agccgctgac
     1741 gggcatcctg ccgagataat ggatctgagt tttgctgtcc aggtattttc ggctttatat
     1801 ataaaggaca atcataagaa gctggaaaaa aaggtaataa gtgtttcagg ggatatagac
     1861 aaaaagattg cggaaagaaa gcttgaaagt tggggtatca gcattgacag tcttactgaa
     1921 gaacaaagac tgtatcttaa cagctggagg atttaggaga atacatggat aacgcaaata
     1981 gggtagaaag aagaaacaaa aaggtaatga ggctacttgt cgtttttctg tcaatagcag
     2041 tagttgtggc cggtgctttt ttgataatgg gcgcttataa aaaccagaac aggatagaac
     2101 agataatagc caaagcggac aaacttcaaa ggggctactt ttatgaaaag gcaatagatg
     2161 tattgaagga agacaagagt atttatgacc ctcatgatca agatgatccg gttacacaaa
     2221 aaataaacga gataaaaaag gcagaagaag accttgtgaa atacgatgga tttgtggaac
     2281 atatcttttt tcacagtctg atcgtgtatc cccaactggc atttgatgac aagggacatc
     2341 cggctattgg atacaatgca tggatgacaa ctgctgacga attcaagaaa atgcttccct
     2401 tgctgcaaga aagaggatat atactttatc ccattgaaga agtatgcgat atagataaag
     2461 agacgggcaa ggtaactaaa aaagatatct atctgccaaa gggcaaaaaa cctctgataa
     2521 tatccattga tgatgttgat tattatgatt atatgaaaca ggacggtttc gccagcagac
     2581 ttgttgtgga taagagtggt aaagttgcca ctgaggttaa aacaccggaa ggcaaaaccg
     2641 agatcaccta tgacggagat gtcatgccca tactggacag atatgttgag aaaaacccgg
     2701 aattttcatg gcagggatac aaggggatag ttgccacaac aggatatcag ggagtattcg
     2761 gatacaggat aacagacgga ctgcctaaga cggatgaatg gagacaggaa gtaaaagaaa
     2821 tatcgaaagc attgcgggaa agcggatggg atatagcatg ccacagttat acccatgacg
     2881 gatacttgaa ggatgcggat gtttcccttg gatccatgaa gtcagatatt gacagatgga
     2941 aatcaaacat agaaccctat gtgggcgaaa ctgcaatata tatttctcct tatgggtttc
     3001 agtatatgtc aggaagcaca gcccatagat atatagtgga ttccggattc catattttct
     3061 gtcctgtcag caatataaga aagctttcat tctataaaga tgtgatggta tcccccaggg
     3121 tgaatatgga cggatacaag atgatgctgg ctcccgatga ggtgaatgaa tattattttg
     3181 acgtggataa agtactggat gatgcaagac ctccattcga tgcaggatcg ttggtataaa
     3241 agcataagaa ggagaagaca tgaattatgc aaaaaggctc agctggcttg aagaccagtc
     3301 tctgctaaaa gcttttcagc tggcggaaac agaagatatg atatcatttt ctgcgggatt
     3361 cccctcatct gaaacatatc cccttgatgc agtgaaggaa tccatggcaa gagtaattga
     3421 taatcaggga gaaacagcat taagttactg ttctaccagt gggtacagca aattaaggca
     3481 gatccttaca aagcgtatgt cggacaagtt caatcttgaa tatgcgctgg atgaaataat
     3541 aataacaagc ggatcacaac agggacttga tatgagtggt atgctttttg tcaacgaagg
     3601 agatgtggtg atttttgagg agccctccta tctgggagcg gtaaatgcac ttcgggcata
     3661 tcaggcagag ctggtatcgg tgccaacgga cagggaagga atggaaatac cggctcttaa
     3721 ggagaccctt gaaaaatacg gaacaagagt aaaaatggta tatgtgaatc cggattttca
     3781 gaatcccacg ggcagaagct ggtccgatgg ccgaagaaaa gactttatgg agttcatgag
     3841 cggctatgat atacccgtcc ttgaagatgc tgcatacggg gagctttcct ttgatgaaga
     3901 aaggcaaaag cccctggcat attatgatca aaaagggcag gtagtatata taggaacttt
     3961 ttcaaaaata ttctgcccgg gattaagagt ggcatggctc tgtgcccgaa gggatataat
     4021 ggagaaatat ctggtactca aaaatgcggc ggatctttct tcctccacca ttgcacagat
     4081 gcagatggcg gattatatgg aaaataacga cctggattta catataaaaa gtatttgtac
     4141 tctttataaa cacagacgtg atgtgatggc caagatgata gattccgaat ttcccaaaga
     4201 agtcaactat gttcttccca aaggagggct gtttatatgg ctggagttgc cagagggtaa
     4261 agatgcggcg gaacttctta ttcgcgcagc acaaaaaaaa gtggcattca taacgggagc
     4321 atcattttat ctcagcacaa agaaaaacaa cgaattaaga ctcaattttt cgaatatgtc
     4381 tgacgaaaaa acagaaatag gcatgaagat cctgggagcg ataactaagg agtatttaaa
     4441 tgtctaaatc agtattgacg gagattttca acataaatga aaacatcaaa aaacgaataa
     4501 aagatgcggt cagtgaatgc ataagggatt tccagagcag cagggaatgc agcacagggt
     4561 tcggagaacc ggtaataggc tatgttagcg caaaacatcc tctttttatc aaataccttg
     4621 atgaagatat atgccttcac ccaaagggca tatatagacc cgggaataca gtcatcatcc
     4681 attttgtacc ttttgaaaga aaaacagccg aaagtaacag aggaggcaag gaaccttcac
     4741 cggagtggac aaaagccttt tatgacagta tgcatctttc gatgaaactg aacggtatca
     4801 tatgcgatac ccttgatgaa gtgggaagac ttcattccgg taccaacata cccacagact
     4861 ggaacgaaga gaagtgcaga gaggagtggt cccataaact ggcagcatat gctgcgggta
     4921 tgggaagatt cggcattgca ggatcctttc acacggaact gggttttgcc ggaagattgg
     4981 gcagcatact ggtggatgaa cactatgcgg actttgaaga aagggatctg aaaatgtggg
     5041 atatggagcg agttataagc cacataaaaa ccgacagtaa atataaaggg gcagaagaca
     5101 tcagagtcag tcggaaagcc atagaagcat gtcccggcgg ggcaatatca gaagatggca
     5161 tagacaggaa gctctgtcag gcctggtgca ggaaaataaa tgatattatt ccttctcccg
     5221 aggtctgtgg caaatgcttt ttctatgaca gataatccat tattcgggaa cgaactttcg
     5281 cttcacccat tatattctgt atagtccaaa tatccggaga ctggcttcgg cctgtgatgg
     5341 ctattcgaat cacagtgctg acatgaccca catgcccctt gtaggcatcc gggtcttttt
     5401 tatagtcctt cggccgggca gcatatgaaa attcagatgc aatatccctt atttttgaga
     5461 accatgtgct ctgatcatcg caagggtcat aggtggagag atatgccttt aggatagaga
     5521 ttgcatcccg gctgtctact tgttcgggca gccggtcttc tatttcgaaa aacgcgtcaa
     5581 aaaagtattt tatgtactcg aacatctgac ttgcacaaat aaagtctttt cgcggctttg
     5641 ggtcgtttct gcctatatcg ataatggatt ccaaatagcc ctcattctct tttagagcag
     5701 catatacctc gggtctgtat tgagaagccc atttttcaag gaactcccta agggaagctg
     5761 ccgggatgcc ggcaagacat tcctttgaaa cgtcatttaa tttatcaaga tcgaaaagtg
     5821 caccggaagt actcattttt tccatggaaa agggaaactc ttcacagggt atatcgggat
     5881 tttgggttcg ccattcttca taatttgaat ttagcagtgt aagcagatat tctcttactg
     5941 cttcgggatg atagcctttt tctttgtaga aatcaagaga aagttcggga tcattgcgtt
     6001 ttgaaagttt tctcttattg ccgttttcgt ccagtttcat taactgtgca gtatgacagt
     6061 aaatgggagg ccggaagttg agtttttcaa aaagttcaac atgtatcggc agcgatgaaa
     6121 gccattcttc gccgcgaacc acatgggtag ttctcataaa atgatcgtca caaacatggg
     6181 caaaatgata tgtgggcatg ccgttggctt tcagtattac tatgtcctga tagttggacg
     6241 gcatggtcag gatacctctt attgcatcat tgatttctat attgttcttt tcttccccgt
     6301 caaaagcccc cgatccaccg gatcttagcc ttagaacata gctttttcct gcagatatat
     6361 gtttttctac ttctgtgatc gaaagatcgc ggaaggtggc atatttaccg taaataccgg
     6421 gagtctcttt tttttcttcc tgctgttttc tggtttttgc gatttcttct tcagtcatga
     6481 aacagggata tgcagccccc ttttttacaa gttcttttgc aaaacaactg tacagatcct
     6541 ttcgcctgct ttgaaaatat gggccgtagt tttcggatcc tgttccggca cctgcaccct
     6601 catcgaaatt tatcccaaaa aaatcaagag aatcaatgat ggtctcaacc gcaccttcaa
     6661 caaatctttt ttcgtctgta tcttctatcc tgagataaaa aacaccgccg cttcggtgcg
     6721 caagcctctc atcgataaag gcaccgtaaa gattgccaag atgtataaag ccggtgggac
     6781 tcggacccag tcttgttatc atagctcctt taggcagttc tctttcgggg taaagcgttt
     6841 cgtagtattc cggtgttctt tcaacctccg gaaaaagaat ttcggcaagt ttgttgtgat
     6901 ccataaagtc ctccgttcca tgtaaaaccg atatttccac attggttatt atatacgaat
     6961 agtaaagata aaaaaagcat tgtttgaact aaaggaaaag ctgctcgtca aattgagtag
     7021 aaaagactgt tgaaataaaa aatcccttag ttccaagaca gactttctta aatatgctta
     7081 tttgacgaaa accataaaag tgaggtataa tattacacat tagttaggag ggatgtcctt
     7141 atggacagtc acagtataac ggcgatagcc gctataattg tgcttgtgat tttgtcggga
     7201 tacttttcag cgacagagac tgcatttaca tcgctgaacc ggataaaaat aaagaatctt
     7261 gccggggaag gcaataaaag agcaaagcgt gtgctacatc tttctgagcg gtacgataaa
     7321 ctgttgtcaa ctattttgat tgccaataat gtggtcaaca tcaccatgac cgccgtggca
     7381 acggtgcttt ttataaatct ctatggagac atgggtgcga cgatctcaac catcgttata
     7441 accattgtcg ttttgatttt tggagaaatt tcaccgaaga ctatggcgaa agaagctcct
     7501 gaatccttca gcatgttttc agcacccata atacattttt ttgagacact ttttacgcct
     7561 gtgaatttca tttttgccaa atggaagcag ctcataataa agatattcaa aataggggac
     7621 agcagcacca taactgaaga cgaactgaaa accatggtgg aggaggcgga gacagaaggc
     7681 ggtatagacg aagatcagag tgaactgata cagaatgcaa tcgaattcaa tgaacttcag
     7741 gccagggacg tacttacccc cagagttgat atggaggccg tggatattga agccaccgaa
     7801 gaggaagtcg cttcgaaatt cagaagtacg gggtacagca gacttccggt atatgaagat
     7861 gatctggatt ccataatggg agtccttaat cagaaggatt ttcataatta catagtcggc
     7921 acagagaaaa ccgtttctga ctttgtgaag cccgttgttt ttgtcggaga gacgatgaaa
     7981 atttctgcgc ttctcaggaa acttcagctg gtgaagaccc atatcgccat aattgtggat
     8041 gaatacggtg ggactgaagg ccttgtaaca atggaagaca taattgaaga actggtgggc
     8101 gagatatatg atgaacatga tgccataatg agccaggaag tcacagaact tcagaacggt
     8161 agttacaggg tcatgtgcag tgcaagcctt tcaaaagtat tcgacttttt cgggatatat
     8221 gatgaacttg atgtaaatac cgtaaacgga tgggcagtgc tttgccttga caagctgccc
     8281 gaaaaaggag acatatttga atacaaaagc ggaaaaaaac tgcttaaggc aagagtcaca
     8341 aaggctaacg gcaaaaaagc tctggaactt aatgttacag cagaagaact gacacgggaa
     8401 gaggaagaag aggtagaaga ataaatggga cttatggaaa aagtctttgg tgacataaat
     8461 gccaaagagg taaaaaagct tgaaaagatc gcggacaaga tagatcttat ggatcgggaa
     8521 atgcagcaac tgactgatgg tcagctgcag gaaaaaactc ctgaattcaa aaaaagactg
     8581 aaagagggag agacacttga tgacatactt cctgaagctt ttgcagtttg cagagaagcg
     8641 gcatggcgga ctcttggcat gaagcatttt tatgttcagc tcataggcgg catagcactt
     8701 catcagggaa ggatagcaga gatgaaaacc ggtgaaggaa aaacactggt ggccacgctc
     8761 cccgtctatt tgaatgcact ggaggagaag ggggtacatg tcgttacggt aaatgattat
     8821 ctggccaaga gagacatgga atggatgggc aagatataca attttctggg gttgagtaca
     8881 ggctgcgtga tacacggcat aacggaagag gacagaagag cagcttatgc agcggatgta
     8941 acatatggga ccaacaacga atacgggttc gattatctta gagataacat ggtcatatac
     9001 aaagaagaga tgacacagag agaccttaac tatgccatag tcgatgaggt ggactccata
     9061 cttgttgatg aggccagaac acctcttatt atttccgggc aaggtgataa atccacagag
     9121 ctttacaatc aggcaaacag attcgtcaaa acactgaaaa acgaaaagga ctatacaatt
     9181 gaagaaaagg acaagcaggt atctctgaca gaagaaggag tttccagatg tgaagagtat
     9241 ttcagagttg ataatttctc cgatccggaa aacatggaaa taaaccatca tgtactgcag
     9301 gcgctcaagg cccataatct tatgaaaaga gatgtggatt atatagtaaa agacggagag
     9361 ataataatag ttgatgaatt caccggaaga ctcatgttcg gaagaaggta cagcgaagga
     9421 cttcatcagg ccatagaagc aaaagagggc gtttatgtcc gctcagaatc aaaaacactg
     9481 gctacaataa ctctgcagaa ttatttcagg atgtatcaga agctggcagg aatgaccggt
     9541 accgcaaaga ccgaagaaaa tgagtttcgt gaaatataca atatggatgt ggtggttatc
     9601 cccacaaata aaccggtaat aagagaagat tgtcaggatg ctgtatttgc cacagaaaag
     9661 ggtaaataca aggccattgc cgacagagtg gctgaggccc atgaaagcgg gcagcctgtc
     9721 ttggtgggaa ctatttccat cgaaaaatca gagttgatta gcgaaatgct gcaaagaaga
     9781 ggagta
//
LOCUS       DAONHK010000036         9383 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_5656_length_9383_cov_4.722877, whole genome shotgun sequence.
ACCESSION   DAONHK010000036 DAONHK010000000
VERSION     DAONHK010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 9383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9383
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5656_length_9383_cov_4.722877"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1552
                     /locus_tag="PLM92_02395"
     CDS             <1..1552
                     /locus_tag="PLM92_02395"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HPR24020.1"
                     /translation="DLTVQLFTLRVHLFNIDPEYHKRAYYAAPIRMRKNEIIGAIGLG
                     VLCEEMNNYMLAMIAVAARTIENEITLLKNNAIIFQHNKEKQSILDSVTDGVIHVDKE
                     QIITQANLQMMEMTGLEKEELIGQHISIVETLPTINSIEKNIEYAEKDLRVKLKSKTG
                     YFSCFLNRNIINEEDPERKSVIWIFTEINEIQELADKISLANRAFFTFKSIIGETRDI
                     KQTVTLAKKVADYEARVILEGESGTGKEILAQAIHNDGKRKNGPFVAVDCGAIPRELL
                     ESEMFGYEEGAYTGAKKGGARGKFELANKGTLFLDEVSNLPLEMQAKLLRALQENKIV
                     RIGGDRPIPIDAHIIAATNKDLKKEVANGTFREDLFYRLDIVHIKVPALRERKADIPL
                     IVNNYIKINSARLHKKIKGLDEEAMKTLMAFNWPGNVRQLNNVIERMMILTDKEIMTK
                     DAIPPEIKSPSSETFYEAIRSDDIDSLDVVNTKYIRRVVEQFGGNIKKASEKLGISRA
                     TVYRYLEK"
     gene            1555..2934
                     /locus_tag="PLM92_02400"
     CDS             1555..2934
                     /locus_tag="PLM92_02400"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPR24021.1"
                     /translation="MSEKKNNQKNSYDMTIMNGTVIDVVEEKYVKKNIGIVNGKIEKL
                     TTEKISGKEVIDAKGLLISPGFIDFHSHVDGNSYSAACLVKQGGTTTLGGERELSSKS
                     INRVEDGFVINQGFSISQSFVLRRAVGITNPKRPANSEEISAMVDLARRFMEYGAFAI
                     CFGLELVPGTSFDEILALSKVAKEYERPIMVHLRKDGKEALQYFEEIIQVAELTGVSV
                     QILQLMYMVGIGGAMPMALEIIEKARAKGLDIMADSGVYAAYSACIGTGIFEKGWEKE
                     YGDTSTEDLLISSGIHVGEYCNDELFETLRSEFPETLVTAFVCDPEAISMALKKDYVY
                     VSTNAADGPHYPGIGAPEVAGTYPRLLGKYVRGKKVISLMEAIKKITILPAKRFGIRD
                     IGYIDEGMNADIVIFDENTIIDRADFIGIGRPDTPPEGIKAVIVNGKKAVENGCLTEN
                     IHAGCFIKR"
     gene            3195..4355
                     /locus_tag="PLM92_02405"
     CDS             3195..4355
                     /locus_tag="PLM92_02405"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003386828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HPR24022.1"
                     /translation="MTLSKYSFALPTRIEFGEGVVNDAGKEAKALGASKVMLVADKGV
                     IAAGLTEAVEESLKKENIDYVIYDQIVPNPRDIHCVAGHEFAVKEGVDVLVAVGGGSS
                     MDTAKAIGTLLTHGKTIQDWCGYQLLEREITPLIAIPTTAGTGSEVTPFVVITDTTTH
                     VKLNIFDPKAAAKVALIDPVMLMKLPSHIMASCGVDALTHAVEAYTCTLACPHTDAYA
                     LYAIELIRKNLRNAVKEPTIEACTGMMLGSTIAGIAFGYSDVGGVHCIGEALGGRYDT
                     PHGVAMAIFLPAVTEYNIPADLEKHVDIAKAMGVDVTGMNKEDAAMEGVKAMQRLCDD
                     VKIPRMKDLKGIDPADFEAIAIASEKNVSTPSNPRKITAKEYLEIIKKVYEG"
     gene            4383..5828
                     /locus_tag="PLM92_02410"
     CDS             4383..5828
                     /locus_tag="PLM92_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015538602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HPR24023.1"
                     /translation="MEEKIKLKRILGFPATYGAAVGLVVSGTAMFSVGNVGAISGNAT
                     FICAAIALIPMMSTAFAFGELTAMLPGGGMISDYTAPALGRFWATFALLSGYVVLIAC
                     DGGTQLVMGGLAFEDLIGVPQAAVSIALLVIIICVNVFGVQFYGGAQAAITWTMMIVF
                     LILGIVGSLGTGEALGVATPIPENAGLLPEGGWPTVFGAVGTAVWFFIGFEFACPLAE
                     ENKKPYKYVPWALILGLITIYVVDIIFVYGAVKYTDLGVMATSSVPHVDAASAVLGPA
                     GGIIMGLLTIFASFTTGNDYVCALPRMLYGMARENLVPKVFAKLHPKYRVSMYGIFFT
                     VCLILLVLIYVTVKGGTSDAILTLINTACITWLIAYIIAMVDVLVLRKKYPDFPRLFK
                     VPAAGIVLPIGMLGAVYAIYTLSYVLMFACILMAIIAAYAIIWNKAHGLPVNDKVPIE
                     KLVKDVRDRSEYLAVWDEAVEEWMKKRDALQ"
     gene            5910..6599
                     /locus_tag="PLM92_02415"
     CDS             5910..6599
                     /locus_tag="PLM92_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPR24024.1"
                     /translation="MEMLLTTPKNERIGSRRIKRKEPGPGPLNNRADRKLTFVADMFT
                     VAMGDNFLELKKTILHREIICRGYRYLTNLSLDYDKYVKRIWARVWELFFKTTFDVSG
                     YSEAENEKCHFVAVNKGKWSISDAEWIDKSSSCSTDKTKEYLSRLNNKLIIERMVMLD
                     MVDAEIIFSPQNRTWTVKYKSVIGSTNWIMIPPITQLIKHKPYEVIRTIEFFELVLDA
                     LVNANKAEKIT"
     gene            6885..7271
                     /locus_tag="PLM92_02420"
     CDS             6885..7271
                     /locus_tag="PLM92_02420"
                     /inference="COORDINATES: protein motif:HMM:NF024924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPR24025.1"
                     /translation="MKRLKFNKENCIGCQLCAQVCSAMKEGEYVPSKARIAIETYYED
                     GDLKYKDDFCILCGICVKKCPEGAITMNEYIEVDMAKCTGCGVCAEKCPKHVIRIRED
                     KAYICDTCKGDPTCVKTCPQNALTFE"
     gene            7285..9051
                     /locus_tag="PLM92_02425"
     CDS             7285..9051
                     /locus_tag="PLM92_02425"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HPR24026.1"
                     /translation="MTMTGYQNKVLRIDLSAGKHKTEPLNMEFAAKYVGSKGLAIRYM
                     YEELKPGIDALGIENKLFFTTGPLTGTPVACSGKLSIAAKSPATGTMNDCSIGGHAGI
                     RIKYAGYDMIIFEGKLEKPGYIVIEDDKVKFMDADDLWGKGSHEAESILTKKYGTDYS
                     VLSIGPAGENLSPMACINSDYYRQAGRGGIGAVMGSKNMKAVIVRGTGGVKVPDIEKA
                     TDRIIELLQKDVLQEDNTFVYDSGTTAFLEACNDGGILPYKNFSDTTDPDWKEYNDGV
                     LMEHRKGKRGCGSCGLGCGNFLQIGKAVCEGPEYESISVAGPNAGIKDPEHIVKFNQV
                     ADDLGLDTISAGDTIIWAMEMTERGIHDFGIRFGEADKMISMLEMMAKKEGIGADLAD
                     GVKKASEKFGGTDFAMQVKGLEYPQYEPRGSWGMSMAYAVSDRGACHMRSYAPNEEVF
                     AASIPPYTAEGKGQMVYNLGEFNAVKFSLCVCDFWGTITYEIMAELLTIVTGREWKPE
                     EMAEVGRRVLNIGRAFNQREGFARKDDTMPERLAKDPLKSGPAAGQTIPREAFDDMLD
                     QYYKVMGWDENGCLPDELIETL"
     gene            9060..9299
                     /locus_tag="PLM92_02430"
     CDS             9060..9299
                     /locus_tag="PLM92_02430"
                     /inference="COORDINATES: protein motif:HMM:NF025807.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPR24027.1"
                     /translation="MEITITLRGALKKYLDGDNERKVIVPNGCTCDEALKVIGLDHKK
                     VARFGFVAVNGMKVMINDRLKEGDELKAYPRISGG"
BASE COUNT         3155 a         1627 c         2274 g         2327 t
ORIGIN      
        1 tgatttaaca gtacaattat ttactttgcg tgtccactta tttaatatag atccagaata
       61 ccataagaga gcatattatg ccgcacccat aaggatgcgc aaaaatgaaa taatcggggc
      121 aataggactt ggtgttcttt gcgaagaaat gaataattac atgttggcta tgatagcagt
      181 tgccgccagg acgatagaaa acgaaataac acttttgaaa aacaatgcca taatatttca
      241 gcataataaa gaaaagcaaa gcatactgga ctctgtaacg gacggagtca tccacgtgga
      301 caaagaacaa ataataacgc aggcaaacct tcagatgatg gaaatgaccg gtcttgaaaa
      361 agaagagctt attggacagc acataagcat cgtagaaaca cttcccacca taaacagtat
      421 cgaaaaaaac attgaatatg cagaaaaaga tctgagggtc aaacttaaaa gcaagaccgg
      481 ttatttcagc tgcttcctta acagaaacat aataaatgaa gaggatccgg aaaggaaaag
      541 cgtaatatgg atatttacag aaataaatga gatacaggaa ttggcggaca aaataagtct
      601 ggccaatcgt gcatttttta ctttcaaaag tatcataggg gaaacaagag atattaaaca
      661 gacagttact ctggcaaaaa aagtagcaga ctatgaagca agagttattt tggaaggaga
      721 aagcggtacg ggaaaagaaa tactggcaca ggcaatacac aatgacggca aaagaaaaaa
      781 cgggcccttt gttgcagtgg actgcggagc catacccaga gaattgctgg aaagcgaaat
      841 gttcggatat gaagaaggag catatacagg agcaaaaaaa ggtggtgcaa gaggcaaatt
      901 tgaactggca aacaaaggca cactttttct tgacgaagta agcaatcttc cccttgagat
      961 gcaggccaag ctcttaagag ctctacagga aaataaaata gtaaggatag gaggggacag
     1021 acctataccg atagatgcac acataattgc ggcaacaaac aaagatctca aaaaagaagt
     1081 ggcaaacgga acattccgtg aagatctttt ttaccggctt gacatagttc atataaaagt
     1141 tcccgcgtta agagaaagaa aggcagacat accgcttatt gtaaacaact atatcaagat
     1201 caacagtgcc agacttcata aaaaaatcaa gggacttgat gaagaggcaa tgaagaccct
     1261 tatggcattc aattggccgg gtaatgtaag acagctcaat aatgtaatag aaagaatgat
     1321 gatattgacc gacaaggaaa taatgacaaa agatgcgata cctcccgaaa tcaaaagtcc
     1381 atcttcggaa accttctatg aagccataag gtcggatgat atcgattctc ttgatgtggt
     1441 caatacaaaa tatataagaa gagttgttga acagtttggc ggaaacataa aaaaagcatc
     1501 tgaaaaactg ggtataagca gagcaacagt atacaggtat ctggagaaat aaatatgtcg
     1561 gaaaagaaga acaatcaaaa aaactcatac gatatgacta ttatgaacgg cacagtaatc
     1621 gatgttgtcg aagagaagta tgttaaaaaa aacattggca tagtaaacgg gaaaatcgaa
     1681 aaacttacaa cagaaaaaat atcgggtaaa gaagttattg atgctaaggg actgcttata
     1741 tcaccgggat tcatagattt tcattctcat gtggatggca attcatattc tgcggcctgt
     1801 ctcgttaagc agggagggac caccacccta ggcggagaaa gagaactcag cagtaagagt
     1861 atcaaccggg tagaagacgg ttttgttatc aatcagggat tttccatttc tcagtcattt
     1921 gttttaagga gagctgttgg tataacaaac cccaaaaggc ctgcaaattc tgaagaaata
     1981 agcgccatgg ttgatcttgc aagaagattt atggaatatg gtgcctttgc catatgcttc
     2041 ggcctggagc tggtgccggg cacttccttc gatgagatac tggcactttc aaaagtggca
     2101 aaagaatatg aaagacccat aatggtccat ttgagaaaag acggcaaaga agccctgcag
     2161 tattttgaag agataataca agtggcagag cttacaggtg taagtgtaca gatacttcag
     2221 ctcatgtaca tggtaggcat tggaggagcc atgccaatgg cccttgaaat aatagaaaaa
     2281 gccagagcaa aggggcttga tataatggca gacagcggtg tatatgcagc ctattctgcc
     2341 tgtataggta ccggcatatt tgaaaaaggc tgggaaaaag aatatggaga tacgtcaact
     2401 gaagatcttt tgatttcttc cggtatacac gtgggagaat actgtaatga tgaacttttt
     2461 gaaacactaa gaagtgaatt ccctgaaaca ttggtaactg cctttgtgtg tgatccggag
     2521 gccatttcca tggctctaaa aaaagattat gtttatgtat caacaaatgc cgcagacggg
     2581 cctcattacc ccggaatagg agctccggaa gtggcaggca catatccaag gcttttgggc
     2641 aaatatgtga gggggaaaaa agtgatttcc cttatggagg ccataaagaa aataacgatt
     2701 ttgccggcaa aaagattcgg tatcagggac ataggatata ttgatgaagg aatgaatgcg
     2761 gatatcgtta tttttgatga aaatactata atcgataggg ctgattttat cgggatagga
     2821 aggcccgaca ctccaccgga aggaataaaa gcagtaatag taaatggaaa aaaagccgtg
     2881 gaaaatggat gtcttacaga aaatatacat gcaggatgct ttataaaaag atgatgtgta
     2941 aaataattag acaattgatt cctgtactat gaaaagaatt ttgctaaaag tatcattgtg
     3001 tataataatt ttgcataaat agcatcgctg tatttttttt gaacaaaaaa aaatactgaa
     3061 tattgttaaa aatataactc tcctttaaaa atgaacactt tctgaccaca tgatagcttg
     3121 gcacagaagt tgctaatcta ttatatgtag cacttagtat gctaaagagt tttttcagaa
     3181 aggagaggaa gaatatgaca ttatcaaagt attcatttgc actgcctacc cgtattgaat
     3241 ttggagaagg tgttgtaaat gatgcgggga aagaagcaaa ggctttgggc gcatccaagg
     3301 taatgcttgt tgcagacaaa ggagtaatag ctgcaggcct gacagaagcc gttgaagaaa
     3361 gcctgaaaaa agaaaacatc gattatgtga tctatgatca gatcgttcca aatcccagag
     3421 acatccattg tgttgcagga cacgaatttg cagtaaaaga aggtgttgat gtactggtgg
     3481 cagtgggcgg cggaagttcg atggatacgg caaaggccat tgggacactt ctgacacacg
     3541 ggaaaacgat tcaggactgg tgtggttatc agcttcttga aagagaaatc acacctttga
     3601 tcgccatacc taccacggcc ggaaccggca gtgaagtgac accgtttgta gttataacag
     3661 atactaccac tcatgtaaag ctgaatatat tcgatccaaa ggcagcagca aaggttgcat
     3721 tgatcgatcc tgttatgctg atgaagttgc catctcatat aatggcatcc tgcggtgtag
     3781 atgcattgac acatgctgtt gaagcataca catgtacact ggcttgtccg catacggatg
     3841 catatgcttt atatgccatt gagcttatac gcaaaaattt gagaaatgct gttaaagaac
     3901 ccactatcga agcatgcaca ggcatgatgt tgggcagtac aatagccggt atcgcattcg
     3961 gatattcaga tgtgggcgga gtacactgta taggagaggc acttggaggg agatatgata
     4021 cacctcatgg tgtggcaatg gcgatattcc ttccggcagt taccgagtac aatatccctg
     4081 cagatctgga aaagcatgtg gatatagcaa aggcaatggg tgtagatgtt accggaatga
     4141 ataaagagga tgcggccatg gaaggtgtca aagctatgca gaggttgtgt gacgatgtga
     4201 aaatcccgag gatgaaagat ctgaaaggta tcgatcctgc cgatttcgaa gccatagcca
     4261 ttgcttcgga gaagaatgtt tctactccca gcaaccctcg gaaaatcact gccaaagaat
     4321 atcttgaaat cataaaaaaa gtttatgaag gttagaaaat tttattaagg aggtgaaata
     4381 atatggaaga gaaaattaag ttaaaaagaa tacttggatt tccggccaca tatggagctg
     4441 cggtagggct ggtagtatcg gggacagcaa tgttttcagt gggaaatgtt ggtgcgattt
     4501 ccggaaacgc aactttcatt tgtgcagcca tagcccttat acccatgatg tccacggcgt
     4561 ttgcctttgg tgaacttacg gcgatgctgc ccggtggcgg aatgatatct gattatactg
     4621 ctcctgcgct ggggagattc tgggccacat ttgcgctgct tagcgggtat gtagttctta
     4681 tagcctgtga cggtggtaca caactggtaa tgggagggct tgcatttgag gatcttatag
     4741 gcgttcctca ggcagcagta tctatagcct tgcttgtaat aataatatgt gtcaacgttt
     4801 tcggtgttca attctacgga ggtgctcagg cggccattac ctggaccatg atgatagtat
     4861 tccttattct gggtattgta ggttcgctgg gaacaggaga ggcactggga gtggctactc
     4921 ctatcccgga aaacgcaggt cttctgccgg aaggcggatg gcccacagtg ttcggtgcag
     4981 tgggtacagc agtatggttc tttataggat ttgaatttgc atgtcctctt gcggaagaaa
     5041 acaagaaacc atacaaatat gttccatggg cgctgatatt aggacttata actatttatg
     5101 tcgttgatat aatatttgtt tacggcgcag taaaatatac tgatttgggt gtaatggcaa
     5161 cttcctcagt accgcatgtg gatgcagcaa gtgccgtcct gggtcctgca ggtggtatta
     5221 taatgggact tctgacgata tttgcatcct tcacaacagg aaacgattac gtttgtgcgt
     5281 tgccaagaat gctttacgga atggcaagag aaaacctcgt acccaaagta ttcgcaaaac
     5341 tgcatcccaa atacagagta tcgatgtacg gtatcttttt cacagtttgt ctgatacttc
     5401 tggtacttat atatgttacc gtaaagggcg gcacatcaga tgccatactt acacttatca
     5461 ataccgcatg cataacatgg ctgatcgcat atattatagc catggtagat gtgctggtgc
     5521 tcagaaagaa atatcctgat tttccaaggc tttttaaagt tccggctgca ggaatagttc
     5581 ttcctatagg tatgctggga gccgtatatg ccatatatac actttcatat gttcttatgt
     5641 ttgcatgtat tctgatggca atcatcgcag cttatgctat aatttggaac aaagcccatg
     5701 ggttgcccgt gaatgataaa gttcctattg aaaaattggt aaaggatgta agagatagat
     5761 ccgagtacct tgcggtatgg gatgaagctg tagaagaatg gatgaagaaa agagatgcac
     5821 ttcaataata caaaataata ggttgatcgg ggtcatcgct tctgcggtga ccccgacaat
     5881 caccataaaa atcatcatgg agggaaaaaa tggaaatgct tcttacgacc ccaaaaaatg
     5941 aaaggatagg cagcagaaga ataaagagaa aagaaccggg acccggtccg ctgaacaaca
     6001 gagcagacag aaagctgaca tttgttgcgg atatgtttac agtggccatg ggagataatt
     6061 ttctggaact aaaaaaaacc attttacatc gggaaataat ctgccgggga tacagatatc
     6121 tgaccaatct gagtctcgat tatgacaaat atgtaaagcg gatatgggca agggtctggg
     6181 aactgttttt taaaacaacc tttgatgttt ccggatactc agaggcagag aacgaaaaat
     6241 gtcattttgt tgctgtaaac aaggggaaat ggagtataag tgatgctgaa tggatagaca
     6301 agagttcaag ctgcagcact gacaaaacaa aggaatacct gtcacggcta aacaataagc
     6361 tgataatcga aagaatggtt atgcttgaca tggtggatgc ggagataata ttcagccctc
     6421 agaacagaac ctggacggta aaatataaat ctgttatcgg ttcaacaaac tggataatga
     6481 tccctccaat aactcaactc ataaagcata aaccatatga agtgataaga acgatagaat
     6541 tttttgaact tgttctggat gcgctggtga atgcaaacaa ggctgaaaag ataacttaaa
     6601 ggacacataa atatcacatt ttaatatatg aaaaatccct ctttcgagag gggtttttca
     6661 tttgctgctg gcatctcact ctcataaatc attgacaccc aaccatattt gctagtataa
     6721 taacatatat taaaattcat tgactccgac cttccggagg acaccttgtg cccggggagg
     6781 cgatagggta acaggagcga tcctgctgcc tgccattgtg caaaggagag actgatataa
     6841 agtcttgctt tgcatttttt tatttcatga aaggtgggag aatagtgaaa agactaaaat
     6901 tcaacaagga aaattgtata ggctgccagc tttgtgcaca agtatgttca gccatgaaag
     6961 aaggtgaata tgtgccttca aaggctagga tagccataga aacatattat gaagacggcg
     7021 atctgaaata caaagatgat ttttgcatcc tttgcggtat atgtgtgaaa aaatgtcccg
     7081 aaggagctat cacaatgaac gagtatatcg aagtggatat ggcaaaatgt acgggatgtg
     7141 gcgtatgtgc cgaaaaatgc cccaagcatg ttatcagaat aagagaagat aaagcgtata
     7201 tatgtgacac ctgcaaaggt gatccgacat gtgtgaaaac atgtccccag aatgcgctga
     7261 catttgagta ggaggtatgt aaagatgaca atgacaggat atcagaacaa agttttgaga
     7321 atagatctat cggccggaaa acataaaaca gaacctctca atatggaatt tgctgcgaaa
     7381 tatgttgggt ccaaaggact tgccataaga tatatgtatg aagagttaaa accgggaata
     7441 gatgctcttg gcatcgaaaa taaacttttc tttacgaccg gtcctctcac gggaacgcca
     7501 gttgcatgtt ccgggaaact ttctattgcg gcaaagtcac ctgccacagg aaccatgaat
     7561 gactgttcaa taggcgggca tgcagggata aggatcaagt atgccggata tgacatgatt
     7621 atttttgaag gaaaacttga gaagcccgga tatatagtta tagaagacga caaagtgaaa
     7681 ttcatggatg ctgatgatct gtggggcaaa ggatcccatg aagcagaaag catcctcaca
     7741 aaaaaatacg gaacagacta cagtgttctt tcaataggtc ctgcgggaga aaacctgagt
     7801 cccatggcat gtataaattc tgactactac agacaggcag gaagaggcgg aataggtgcc
     7861 gtaatgggaa gcaaaaatat gaaagcggtt attgtcaggg gaacaggcgg agtaaaggtg
     7921 ccggacatag aaaaagcaac ggacaggata attgaacttc tacaaaagga tgttcttcag
     7981 gaggacaaca cttttgttta tgattcggga acaactgcct ttctggaggc ctgtaatgat
     8041 gggggcatcc ttccctacaa aaacttttcc gacacaactg atccggactg gaaagaatat
     8101 aacgacggcg ttcttatgga acatcgcaaa ggcaaaagag gctgcgggag ctgtggactg
     8161 ggatgcggaa atttcctgca gataggaaag gctgtatgtg aaggtcccga atatgaatct
     8221 atttcggtgg cggggcctaa tgcggggata aaagaccctg aacacatcgt caaattcaat
     8281 caggttgcag atgatttggg acttgacacc ataagtgccg gagataccat aatatgggca
     8341 atggaaatga ctgaaagagg catacatgat ttcggcatcc ggtttggtga agctgacaaa
     8401 atgatcagta tgctggaaat gatggccaaa aaagaaggaa taggagcgga tctggctgac
     8461 ggtgtaaaaa aagcttcaga gaaattcgga ggaacagatt ttgccatgca ggtaaaagga
     8521 cttgagtatc cccaatatga accaaggggt tcatggggaa tgtccatggc ctatgcggtt
     8581 tcagacagag gggcatgtca tatgagatca tatgctccca atgaagaagt ctttgccgca
     8641 tctataccgc cgtacacagc agagggaaag gggcagatgg tttataacct tggggaattc
     8701 aatgctgtga aattctccct gtgcgtttgt gatttctggg gaaccataac ttatgaaata
     8761 atggcggaac tgctgaccat tgtaacggga agagaatgga aaccggagga aatggcagaa
     8821 gtaggaaggc gtgtgcttaa cataggacgt gcattcaatc agagagaagg ttttgccaga
     8881 aaagatgata cgatgccgga aagactggca aaggatccat taaaaagcgg acctgcggca
     8941 ggccagacca taccgagaga agcctttgat gatatgcttg atcagtacta caaagtaatg
     9001 ggctgggatg aaaatggatg tctgcccgat gaactcatag agaccctgta aaatgaaata
     9061 tggagataac aataacactc agaggtgcat taaaaaagta tctggacgga gataatgaaa
     9121 gaaaagtgat cgtacctaac ggatgtacat gcgacgaagc tttaaaggtc ataggactgg
     9181 atcacaaaaa agtggcccgt tttggttttg ttgcagtgaa cggcatgaaa gttatgatca
     9241 atgacaggct aaaagaggga gatgaattaa aggcatatcc caggatatcg ggaggctgat
     9301 aatgaaaaaa agatatgaaa agaatttatc agcttttagt gaagaggaga taaacaaact
     9361 tcattatctt aaaatatgca tca
//
LOCUS       DAONHK010000037         8857 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_6086_length_8857_cov_4.425926, whole genome shotgun sequence.
ACCESSION   DAONHK010000037 DAONHK010000000
VERSION     DAONHK010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 8857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8857
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6086_length_8857_cov_4.425926"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="PLM92_02435"
     CDS             complement(<1..401)
                     /locus_tag="PLM92_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007064675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HPR24028.1"
                     /translation="METHRNIRGKTPVYIQIISDFRQKIIDGELPDGSFLPSERHMSE
                     HLGVHRNTVTKAYNELKAEGLVISLHGVGYKVSYKESYENKREDRTQVKNVNWAHLLK
                     DEYLDIEKSFDDIFSRSYNAKNISFAGGISSP"
     gene            complement(427..3624)
                     /gene="carB"
                     /locus_tag="PLM92_02440"
     CDS             complement(427..3624)
                     /gene="carB"
                     /locus_tag="PLM92_02440"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005354269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HPR24029.1"
                     /translation="MPLNKDIKKVLVIGSGPIVIGQAAEFDYAGTQVCRTLKEEGIEV
                     VLVNSNPATIMTDKTMADKIYIEPLTLSVLKKIIGIEKPDSILPTLGGQMGLTLSMKL
                     AREGFLASHNVRLLGADPLTIDKAEDRQMFKDMLESIGEPVIESRVAERIEDALLYAE
                     EIKYPVVVRPAYTLGGTGGGIANDPEELKVIGTDGLRQSPITQILIEKCVSGWKEIEF
                     EVMRDSRGNTITVCSMENLDPVGVHTGDSIVVAPAVTLADKEYQMLRNASIKIIQELS
                     VCGGCNCQFALDPGSFDYAVIEVNPRVSRSSALASKATGYPIAKVASRIAIGYTLDEI
                     KNEVTKKTYACFEPALDYVVVKFPKWPFDKFVYAKRTLGTQMKATGEVMAIGSFFEQA
                     LMKAVRSIELGLDTLNLKKLKEKTDEEIKNLLYNCDDERIFVVYEAFKRGVGLDEIYE
                     ITGIDRWFLSKINNLVYMEMFLEEGELTKEKYKDAKDLGFLDSTIKKLSGQEPPEKIP
                     EAFKMVDTCAAEFAAETPYFYATYDKENEAADHIKKNDTGRKKIIVFGSGPIRIGQGI
                     EFDYCSVHCVNALKESGYEAIIVNNNPETVSTDFDVSDRLYFEPLTPEDVDGIIETEK
                     PWGVIVQFGGQTAIKLTKYLLKKGVNILGTSADSIDEAEDRKRFDSLLQKCGIPRPRG
                     ETAYDTATAVRKADILGYPVLLRPSYVLGGQNMIIAYSKEDVVEYMDIITANIELENP
                     VLIDKYLMGKEIEVDAVCDGEDFVIPGIMEHIERAGVHSGDSISVYPPQSLGDDIIKK
                     IVKYTGILATELKVVGMVNIQYVLYEGELYVIEVNPRSSRTVPYISKVTNVPVVRLAT
                     KVLLGGKLSELGYKNGLAPVGEYVAVKVPVFSFPKLTDADSQLGPEMKSTGEVLGIDK
                     DFESALYKGLLGAGYGLTKVDEIVLITVKDGDKPEAVPLAQKFIDLGYSIAATEGTAR
                     YFNSHGIPAIKINAFDEPRPNTDTLFDSGAVDFVVSTSIAGRKPAMRSVQLRRKAIER
                     NIVTLTSIDTANALANCLISGKNLGNVNMVDITEI"
     gene            complement(3611..4735)
                     /locus_tag="PLM92_02445"
     CDS             complement(3611..4735)
                     /locus_tag="PLM92_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006599708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HPR24030.1"
                     /translation="MVSSSKMAYHKYEPAWLILEDGTVFEGRSLGCGGTAVGEVVFTT
                     GMTGYQETLTDPSYYGQIVAQTFPLIGNYGVNDTDRESGRLWLKGYIIREWCYEPSNF
                     RSKSTLGEYLEKHNVVGIYDIDTRALTRKIREHGVMNGAVTTEYPDDTGAFLELLKDF
                     VISDAVISVSDKKPSFHKAQVSKYHVALMDYGYKSNILRSLSELGCDVTVMPWDSDKA
                     AIEKVAPDGIMLSNGPGDPAENKQIINNLKGILKTDLPVLGICLGHQIMALAFGCRTQ
                     KLKYGHRGANQPVMDIEKGRIFVTSQNHGFAVVKESVTEEIGHISHYNVNDGSCEGIE
                     YKRPDTFTVQFHPEACAGPHDTRYLFDRFIESMEKKNAAE"
     gene            complement(4860..5984)
                     /locus_tag="PLM92_02450"
     CDS             complement(4860..5984)
                     /locus_tag="PLM92_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011989003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HPR24031.1"
                     /translation="MKTELIATATFGLEAVVKREIETLGYRILKSENGKITFLGDERA
                     IVRSNLWLRAADRVLLKMAEFQAEEFEELFQQIKGIPWEELIPPDGRIIVTGSSVKSK
                     LHSVPACQSIANKAVIKRLSEAYGTEEFEETGAEYSIKLTLLKDRVTVTVDTTGKGLH
                     KRAYRIMGTEAPLKETLAAALVMLSFWKEGRMLADPCCGSGTIPIEAAMIGKNIAPGL
                     NRDFVSEGWHIIEDSIWKEERKKAFGMINNDADLRIEASDISETALQAAVANAKEAGV
                     DDVITFRRADIADFDTEEKKCVIITNPPYGRRIGSKEDIDRIYKVLKNLLEKNKGWSL
                     FIVTADKGAEKAIKGRPADKRRKLYNGRLEVCYYQFHGEK"
     gene            complement(6041..7063)
                     /gene="mreB"
                     /locus_tag="PLM92_02455"
     CDS             complement(6041..7063)
                     /gene="mreB"
                     /locus_tag="PLM92_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004618548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HPR24032.1"
                     /translation="MGFTEDVGIDLGTANVLVYIKGKGIVLNEPSVVAINNDTNEILA
                     VGEDARQMLGRTPANIIAVRPLRDGVISDYDVTERMLKYFIKKTCGSGKFFRPRIMVC
                     VPSGVTEVEKRAVREAATQAGGKDVFLMEEPVAAAMGAGLDISKAGGTMVMDVGGGTT
                     DIALISLGGIVASTSVKIAGDRFDEAIIKYMRKKHKLYVGERTAEELKKGIGTAYPRD
                     EEVSMTCSGRDLVTGLPRDVSMTSTDMLEAMEEPLQAVCEAAHSVLERTPPELAADIS
                     DSGLVLTGGGAMLWGIDKRITQRTGLKVAIAEDPTSCVAIGTGKALDNISIIENSRLN
                     KRHAYI"
     gene            complement(7168..8781)
                     /locus_tag="PLM92_02460"
     CDS             complement(7168..8781)
                     /locus_tag="PLM92_02460"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014270454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HPR24033.1"
                     /translation="MKKQKKYIFITGGVVSGLGKGITAASLGRLLKSRGQKVVSQKLD
                     PYINVDPGTMSPYQHGEVYVTEDGAETDLDLGHYERFIDEDLNKFSNLTTGKVYWNVL
                     NKERKGEYLGNTVQVIPHITNEIKEFIYSVDKQNDADVIITEIGGTTGDIESQPFLEA
                     IRQVSLEQKEENCLFVHVTLVPYIKSSGEHKSKPTQHSVKELRSMGISPDIIVIRSDE
                     PVSIDIKEKISLFCNVKADCVIENLTVPVLYEAPVMLEESGFSKIVCRELGLKTGSCH
                     MKDWQEMLRRIENRRDTVKIAITGKYVKLHDAYISVAEAIKHGGYENGCRIDISWVEA
                     EDVTEDSVTRIFKDMAGILVPGGFGDRGIEGKILAAKYARENNVPYLGICLGMQTAAI
                     EFARNVLGLSDAGSGEFGEGTGNKIIDLMPDQYGDIQKGGTMRLGAYPCVIEKDTIMH
                     RAYGKENICERHRHRYEFNNDYRERFVAAGMKIAGASPDGKLVEAVEIPGNDFFVGVQ
                     YHPEFKSRPNRAHPLFREFVRATIEKDLK"
BASE COUNT         2018 a         2269 c         1657 g         2913 t
ORIGIN      
        1 ggagatgata tgcctcctgc aaaggagata ttttttgcat tatatgatct tgaaaaaata
       61 tcgtcaaaag atttctcaat atccagatat tcgtccttca gcaaatgcgc ccagttgaca
      121 tttttgactt gtgttctgtc ttctctctta ttttcatagc tttccttgta gctgaccttg
      181 tatcccacac catgtaatga gataacaagt ccctcggctt tgagttcgtt gtaggccttt
      241 gtgaccgtgt ttctgtgtac accgagatgc tctgacatat gtctttctga aggcagaaaa
      301 gaaccgtcgg gcagttcacc atcgattatc ttctgtctga agtccgaaat tatctgtata
      361 tatacaggag tttttcctct tatgtttctg tgcgtctcca ttgcctgctt ttcttgcctt
      421 ttttcctcat atttcagtta tatccaccat gttaacatta ccaagattct ttcctgaaat
      481 caagcagttt gccagggcat tggccgtgtc tatagatgta agtgtgacga tattcctttc
      541 tatggctttt cttctaagct ggacagatct catagccggc tttcttccgg cgatggaagt
      601 tgatactacg aaatccactg ccccgctgtc aaaaagtgtg tccgtattag gccggggctc
      661 atcaaaggca tttattttta ttgctggtat cccatggctg ttgaaataac gggcagtgcc
      721 ttctgttgcg gcgatactgt atcccaggtc gataaatttc tgtgcaagag gaacagcttc
      781 gggcttgtca ccgtctttta ccgtgatcaa aactatttca tccaccttcg taagaccgta
      841 tcccgctccc agaagccctt tgtataatgc cgattcgaaa tctttatcta tgcccagaac
      901 ttcacccgtg gatttcattt caggtcccag ttgactgtcg gcatccgtaa gcttgggaaa
      961 actgaaaaca ggaactttca cagccacata ttcacccacg ggagccagac catttttata
     1021 tcccagttcc gaaagcttac cgcccaaaag cacttttgtt gccaatctta ccacggggac
     1081 attggtcact ttgcttatgt atggtactgt ccgtgaagat ctgggattca cttctattac
     1141 atacaattct ccttcataaa gcacatactg aatatttacc atgcccacta ctttcagttc
     1201 cgtggccagt ataccggtgt actttactat tttctttatg atgtcatccc caagactctg
     1261 gggaggatat accgatattg aatctccgga gtgtacgccg gccctctcta tatgttccat
     1321 tatcccgggg ataacaaaat cctctccgtc gcatacggca tccacctcta tttcttttcc
     1381 cataagatat ttgtctataa ggaccggatt ttccaattca atgttggcag ttattatgtc
     1441 catgtactca acaacatctt ctttgctgta tgctattatc atattctgtc cgcccagaac
     1501 ataggatggc cttaaaagca ccgggtatcc cagaatatcc gcctttctta cggccgttgc
     1561 tgtatcatat gccgtttcac cccggggtct tggtataccg cacttttgaa gcaggctgtc
     1621 aaaacgtttc ctgtcttcgg cttcatctat gctgtcagct gatgtaccca gtatattaac
     1681 accctttttg agaagatact tcgtaagttt tatggctgtc tgtccgccga actgtactat
     1741 cactccccag ggtttttctg tttctattat accgtccacg tcctcgggag tcaggggttc
     1801 aaaatacaat ctgtcagata catcaaaatc cgtagaaacg gtttccggat tattgttgac
     1861 tattatggct tcgtacccgc tctccttcag ggcatttaca caatgcacgg aacagtagtc
     1921 gaattctatt ccctgtccta tcctgatagg cccggaaccg aatactatta tttttttcct
     1981 gccggtatca ttctttttta tatgatccgc agcttcgttt tccttgtcat aagtggcata
     2041 aaaataaggt gtttcagcgg cgaattcagc ggcgcatgtg tcaaccatct tgaatgcttc
     2101 cggtatcttt tccggcggtt cctgcccact gagttttttt attgtggaat ccagaaatcc
     2161 cagatccttt gcatctttgt atttctcttt tgtcagctcc ccctcttcaa ggaacatctc
     2221 catatagacc aaattgttta tcttgcttaa gaaccatctg tctatacccg taatttcata
     2281 tatttcatca agtcccacac ctcttttaaa ggcttcataa accacaaata tacgttcatc
     2341 gtcacagttg tacaaaagat tttttatttc ttcgtctgtt ttttctttca gttttttcag
     2401 attcagtgta tccagtccaa gctctatgga gcgtacagct ttcatgaggg cctgctcaaa
     2461 aaaactgcct atggccatca cctcacctgt ggctttcatc tgggtcccca gtgttctttt
     2521 tgcatagacg aatttatcga agggccattt gggaaatttg accaccacat aatcaagggc
     2581 aggctcaaaa catgcataag ttttttttgt tacttcattc tttatttcgt ccagagtata
     2641 tccgatggct atcctggatg ccacctttgc aatgggatac cctgtggcct tggaagccag
     2701 tgccgaagat cttgacactc ttgggttcac ttcgataaca gcataatcaa aacttccggg
     2761 atccagggcg aactgacagt tgcagccgcc acagacggaa agttcctgta ttatttttat
     2821 ggatgcgttc ctgagcatct ggtattcctt gtccgccagg gtcaccgccg gtgccactac
     2881 tatggaatct cccgtgtgca ctcccaccgg atccagattt tccattgaac atacggttat
     2941 tgtgttgcct ctgctgtccc tcatgacttc aaactctatt tctttccagc cggaaacaca
     3001 cttttcaata agtatctgtg ttatcggact ttgtctcaag ccgtcggtac ctatgacttt
     3061 cagctcctcc ggatcatttg ctataccacc gcctgtgccg ccaagggtat acgccggtct
     3121 taccaccacc ggatatttta tttcttccgc atacaaaaga gcatcctcga tcctctcggc
     3181 cacacgggat tctatgacag gctctcctat ggactccagc atatccttga acatctgacg
     3241 atcctctgct ttatctatcg taagaggatc cgcaccaagg agcctaacat tgtgggacgc
     3301 caaaaatcct tcccgagcca gtttcattga aagcgtaagc cccatttgac ctcccagagt
     3361 gggaaggatg ctgtccggtt tttctattcc gattatcttt ttgagcacag aaagagtgag
     3421 gggttctata tatatcttat cggccatggt cttatcagtc attatagtgg caggattgga
     3481 attgaccagt acaacttcta tgccctcttc tttaagtgtt ctgcagacct gggtgccggc
     3541 ataatcaaac tctgccgcct gccctatcac gataggtccc gagcctatga ccagtacttt
     3601 ttttatatct ttattcagcg gcatttttct tctccatact ttctataaat ctgtcaaaca
     3661 aatatctggt atcgtggggg cctgcacagg cttcaggatg aaactgcact gtaaaagtat
     3721 caggcctttt gtattctatt ccttcgcagc tgccatcgtt gacattataa tgacttatgt
     3781 gtccgatctc ctccgttact gattctttta ccactgcaaa accatgattc tgactggtaa
     3841 cgaatatccg tcctttttcg atgtccatca cgggctgatt ggcacctctg tgcccgtatt
     3901 tcaatttctg tgtcctgcat ccaaaggcaa gggccattat ctggtgacca agacatattc
     3961 ccaaaacagg cagatctgtt tttaatattc ccttaagatt atttatgatc tgtttgtttt
     4021 cagcaggatc tcccggtccg ttggagagca ttatcccgtc gggagccact ttttcgatgg
     4081 cagctttatc agaatcccag ggcattacgg ttacatcgca cccaagttca ctcaggcttc
     4141 ttaatatatt gcttttatat ccgtaatcca tgagcgcaac atggtatttg gatacttgtg
     4201 ctttatgaaa cgaaggtttt ttgtctgaaa cggatattac cgcatcagat atgacaaagt
     4261 ccttcaataa ctctaaaaaa gcacccgtat catcagggta ctcggtggtc acggctccgt
     4321 tcatgactcc gtgttctcgt attttccgtg tcagcgccct tgtgtctatg tcatagatcc
     4381 caaccacatt atgtttctca agatattccc ccaatgtgga cttgcttctg aaatttgaag
     4441 gctcatagca ccattctctt atgatgtagc ctttgagcca taaacgtcct gactccctgt
     4501 ctgtatcatt cacgccataa ttccctataa ggggaaatgt ctgggcaact atctggccgt
     4561 aataactggg gtctgtaagg gtttcctgat atcctgtcat ccccgttgtg aaaaccacct
     4621 caccaactgc ggtacctccg cagcccagag aacgtccttc gaaaaccgtt ccgtcttcca
     4681 gtatcaacca agcgggctca tacttgtgat atgccatttt gctactgctg accatttttt
     4741 ctcctttgtg taattttaca cctcaatgta aataattata cacttatact acacgaagtc
     4801 aacggtttta tacgtattta ttgcatttac atgtattttt atgcacggat acaaacatct
     4861 cacttttctc cgtggaactg atagtagcaa acttcaaggc gaccgttata taatttgcgt
     4921 cttttatctg caggacggcc ttttatagcc ttttccgcac ctttatcggc ggtcacaata
     4981 aaaagcgacc agcctttgtt tttttcaagc agatttttga gaactttgta tatccggtct
     5041 atgtcctctt ttgagcctat ccttcttcca tagggaggat ttgttattat gacacatttt
     5101 ttttcttcag tatcaaaatc cgcaatatct gctcttctga aagttattac atcatccact
     5161 cccgcttctt tcgcatttgc aactgccgcc tgcagggcag tttccgatat gtcagatgcc
     5221 tctatcctga gatcggcatc attgtttatc atcccaaagg cttttttcct ttcttccttc
     5281 cagatactat cttctattat atgccatcct tccgaaacaa aatccctgtt aagtccggga
     5341 gctatatttt tgcctatcat tgccgcttct atgggtattg ttcccgatcc gcagcagggg
     5401 tctgccagca tacgtccttc tttccagaat gaaagcatca ctagggctgc ggcaagggtc
     5461 tcttttagcg gcgcttccgt tcccatgatg cggtatgccc ttttgtgcag gccttttccg
     5521 gttgtatcta ctgtcaccgt cacccggtct ttcagcaggg tcagctttat ggaatattca
     5581 gcgcctgttt cttcaaattc ttctgtccca taggcttcgg acagtctttt gatcacagct
     5641 ttgttggcga tactctggca tgctgggaca ctgtgcagtt ttgatttcac ggaactgccg
     5701 gtaacgatta tccttccatc cggcggaatg agttcttccc agggtattcc ttttatctgc
     5761 tggaaaagtt cctcaaattc ttctgcctga aactctgcca ttttcaaaag caccctgtca
     5821 gcggcacgca gccataagtt ggaacgcact attgcccttt cgtctcccag aaaagttatt
     5881 ttcccgttct cactttttaa tatccggtat cccagggttt ctatctccct ttttactact
     5941 gcctcaagac cgaaggtcgc cgtggctata agttctgttt tcattatttc tctcctatta
     6001 ataaaggctg cctctttaga ggcagccttt ctcttacttc tcatatatat gcatgtcgtt
     6061 tgttaagtct gctgttttct attatgctta tattgtccag ggccttgcct gttccgatgg
     6121 ccacacatga agtggggtct tcggctatgg ctaccttaag acccgttctc tgggtgatcc
     6181 tcttatcgat gccccaaagc atggcccctc cgcctgtcag gaccaggcct gaatcactta
     6241 tatctgccgc cagttccggc ggtgtccttt caaggacact gtgtgccgct tcacagactg
     6301 cctgcagagg ttcttccatt gcttcaagca tgtctgtact tgtcatggaa acatctctgg
     6361 gaaggcctgt tacaaggtct cttccactgc aggtcatgct gacttcttca tccctggggt
     6421 atgctgttcc tatgcctttt ttgagctcct cggctgttct ttctcccaca tataatttat
     6481 gctttttgcg catatatttt attatggctt catcgaatct gtcaccggca atttttaccg
     6541 atgtactggc cactatgcct ccaagagaaa taagggcaat atccgtagtt cctccgccga
     6601 catccattac cattgtgccg ccagcctttg atatgtcaag tccggctccc atggcagccg
     6661 ccaccggttc ttccataaga aagacatctt tccctccggc ctgtgtggcc gcttccctga
     6721 ctgcccgttt ttcaacttcc gtaactccgc tgggcacaca taccattatc ctgggtctga
     6781 aaaatttacc cgagccgcag gtttttttga tgaaatattt cagcattctt tctgtcacgt
     6841 cataatctga aatgactccg tctctcaagg gtcttaccgc aattatattt gccggtgttc
     6901 ttccgagcat ctgtcttgca tcttccccca ctgccagtat ttcatttgta tcattgttta
     6961 ttgcaacaac agagggctcg ttcaaaacga tccctttccc ttttatatat acaagcacgt
     7021 tggccgttcc caaatctata cctacgtctt cggtgaatcc catctttgct tatcctcctt
     7081 agcattggtt aaaagtatca ctaagctatt atatataaga acaacctttt tttcaatcac
     7141 tttttgtatg cccttcttac aaaacagtca tttcaagtct ttttcaatgg tcgctctcac
     7201 gaattccctg aaaagaggat gtgctctgtt tggcctgctt ttgaattcag gatgatactg
     7261 cactcctaca aaaaaatcat tacccggtat ctccacggcc tccaccagct taccgtcagg
     7321 agatgcccct gctattttca taccggcagc aacgaatctt tctctgtaat cattgttgaa
     7381 ttcatatctg tgacgatggc gttcacatat attctctttt ccgtatgccc tgtgcattat
     7441 agtatctttt tcaatgacgc agggatatgc tcccaaccgc atggttcccc ccttttgtat
     7501 gtcgccgtac tgatcgggca taaggtctat tatcttgttg ccggtccctt caccaaattc
     7561 tcccgagccg gcatcggaaa gtcccaaaac gttcctggcg aattctatgg ctgccgtctg
     7621 cataccaaga catataccca aatagggcac attattttct ctggcatatt tcgcggccag
     7681 tatctttcct tctatacctc tgtcaccgaa tcctccggga acaagaatgc ctgccatatc
     7741 tttgaatatt cttgtcaccg aatcttcagt tacatcttca gcctccaccc aactgatatc
     7801 tattctgcat ccgttttcat accctccgtg ctttatggct tccgcaacgg aaatgtaagc
     7861 atcatgaagc ttgacatatt tcccggttat tgcgatcttc acagtatctc ttctgttttc
     7921 tatccttctt aacatttcct gccagtcctt catgtggcaa gagcctgttt tcaaccccag
     7981 ctcacgacat actattttgg aaaatccgct ttcctccaac ataacaggag cttcataaag
     8041 gaccggcaca gtaaggtttt ctattacgca gtctgctttc acattgcaga aaagcgaaat
     8101 tttttctttt atgtcaatgc tcaccggttc atccgaacgt attactataa tatccggcga
     8161 tattcccatg gaccgcagtt ccttcactga atgctgggtc ggtttggatt tgtgttctcc
     8221 ggaacttttt atgtaaggca caagagtaac atgtacaaac agacagtttt cttccttttg
     8281 ttccagagac acttgtctta tggcctccag aaaaggctga ctttctatat cgccggtagt
     8341 acctcctatt tcagttatta ttacatccgc atcgttttgt ttgtccacac tgtaaatgaa
     8401 ttctttgatc tcattggtta tgtggggtat gacctgcact gtgttgccca ggtactcccc
     8461 ttttctttcc ttgttcaaaa cattccagta gactttccct gtggtgagat tagaaaattt
     8521 gttcaggtct tcatctatga atctttcata atgcccgaga tcaagatcgg tttcggctcc
     8581 gtcttcggta acatagactt ctccgtgctg ataagggctc attgtaccgg gatccacatt
     8641 tatatatgga tccagcttct gagagactac tttttgtcct ctggacttaa gcagtctccc
     8701 aagggatgct gccgttattc ctttcccaag cccggacaca actccaccgg taataaaaat
     8761 gtatttcttc tgttttttca tttcttctcc tttctctgaa aaacggtctg taaagttaag
     8821 aatgaaaaat gaggcaccaa ccctttgtga tacaatg
//
LOCUS       DAONHK010000038         8326 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_6597_length_8326_cov_4.845847, whole genome shotgun sequence.
ACCESSION   DAONHK010000038 DAONHK010000000
VERSION     DAONHK010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 8326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8326
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6597_length_8326_cov_4.845847"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(89..1822)
                     /locus_tag="PLM92_02465"
     CDS             complement(89..1822)
                     /locus_tag="PLM92_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008428970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HPR24034.1"
                     /translation="MVNLKIDGIKVSVKKGTTLMEAAGKIGINIPHLCYLEGINDIGA
                     CRVCVVENAGTDKLLASCNTMAEEGMDIFTNSHKVRKARRTNVELLLSEHDCLCATCV
                     RSGNCELQKVANDLGILEFPYHWKPERNKWDKDFPLIRDASKCIKCMRCVQICDKIQD
                     LHVWDVNGSGPHVTVGVAEGSDMQKADCSLCGQCITHCPTGALRERNDIDKMYEALED
                     PETVTIVQVAPAVRAAWGEGIGLPKNKATLGRMVNGIRKLGFDYIFDTNFTADLTIME
                     EGSEFLERFKNKENYSWPMFTSCCPAWVRFVKTQYPDMVKQLSTAKSPQQMFGAMIKT
                     YIAKHLGVDPKKIFSVSVMPCTAKKYERGVEQVNDSGYGSDVDIVLTTREINRMMRAS
                     HVNPDTLKNEEFDDVFGIGSGAAVIFGATGGVMEAALRSAYFLLTGKNPDPDAFKKVR
                     GMEGWKEAEFDVSGATVRVAVASGLANTRKLIEAIRAGEVNYDFVEIMACPGGCAGGG
                     GQPINEGEEMAEDRGKILYGLDKVNELRFSHENPAILKSYEEFLGKPLGHKSHQLLHT
                     DLTKWDIEVKQ"
     gene            complement(1816..3666)
                     /locus_tag="PLM92_02470"
     CDS             complement(1816..3666)
                     /locus_tag="PLM92_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003372789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HPR24035.1"
                     /translation="MSRLDIKSQDQAQIVVESLYEDMERRVAASHPGLCPVDTAAAFL
                     RLSHGQSCGKCTPCRVGLEQLENLIDDVLDGGKDVTKDTIGLIRTTAESIRDSADCAI
                     GFEAANMVLKGLDGFLEDYISHVEKGVCASNIREYSEAIPCVGLCPARVDIPGYVSLV
                     LEGRYKDAVKLIKKDNPFPIACALVCEHPCENRCRRTMIDAPINIRGLKRFAVDNSGK
                     VEPPPKAESTGKKIAVIGGGPSGLTAAYYLSLMGHKVKVFEKRKFLGGMLRYGIPSYR
                     LPRERLQEEIDIILSLGVDVELETDISKAEEVKKLKKDYDAVYIAVGAHAFQSLGIKG
                     EDSRGVLSAVEMLRAIGDGNMISLEGKTVSVIGGGNVAMDVARTARRLGAKEVNILYR
                     RRRSDMTALNEEIEGAIAEGCEVVELKAPSRIETDKDGNVKAIWVKPQIPGELDKSGR
                     PRPCDSKGSEERYESDFVISAIGQKIEAGTFDKLGIATDRGRVSADEACIVSKLKGVF
                     AGGDCVTGPATAIRSIAAGKTAAANIDEYLGYNHEISVDVEVPHAKQGNRPPMGRIQM
                     RERPSASRRKDFEEIEEGMTLEEAMQEASRCLRCDHFGMGSFKGGREQKW"
     gene            complement(3786..4769)
                     /gene="selD"
                     /locus_tag="PLM92_02475"
     CDS             complement(3786..4769)
                     /gene="selD"
                     /locus_tag="PLM92_02475"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: protein motif:HMM:NF002098.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HPR24036.1"
                     /translation="MAPGALTQVLRKLPSPDDPALIVGFDNADDASAYMIDEEKVLLQ
                     TVDIFPPVVDDPYTFGQIAAANAVSDIYAMGGKPLLAMNIFCCPEDMPEEIAGEILKG
                     GYDKINEAGAMITGGHTIKDREPKYGLAVTGFVHPRHLLTNRGAKEGDTLILTKPLGT
                     GILTTAAKAGLLGPAETEEMIRSMTSLNNFIAEAVKKYDINACTDVTGFGLLGHCYEM
                     AEASGLTAVIKSKKVPCMKKALELAKEGIIPAGAYANMEYIRCRAKISPDVVEEMSDV
                     MHDPQTSGGLLISMPQKEAEKLLRSIKKSQPYAEAIGFFIAKKEADIIVEP"
     gene            complement(4857..5759)
                     /locus_tag="PLM92_02480"
     CDS             complement(4857..5759)
                     /locus_tag="PLM92_02480"
                     /inference="COORDINATES: protein motif:HMM:NF040786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism-associated LysR family
                     transcriptional regulator"
                     /protein_id="HPR24037.1"
                     /translation="MDFKQIEAFVNVVKYHNFSKAADASFLTQPTISAHISSLEKELG
                     MQLIERGGKEAVPTRQGQLFFKYAVAMIGTREKAVYSLKNYNESIEGVIEIFASNVPY
                     QYILPNLMVSFKNKYHNVKFYVEQEDSGQVNDSIREQKGEIGFTGSRTENDLVYVKLE
                     EDNAVLITPKNEKYSKLHGKPIKMADFMEDEFVWRELGSGSRKAFEKAMTYMGISIKK
                     MDVIARMSNLTGVKKAVSAGLGISVISGLSVERDTEKADYLVFDIEDFKFERSFYMTY
                     HRQTTLSPTAEMFKKHVMEEYSIK"
     gene            complement(5761..7674)
                     /gene="selB"
                     /locus_tag="PLM92_02485"
     CDS             complement(5761..7674)
                     /gene="selB"
                     /locus_tag="PLM92_02485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HPR24038.1"
                     /translation="MNNIIIGTAGHVDHGKTCLIKELTGTDTDRLKEEQQRGITIELG
                     FANMKTEAGENIGIIDVPGHEKFIRNMLSGIGGIDICLMVIAADEGVMPQTMEHLEIL
                     STLRIKKGVIALTKADLADEEWIEAVKEDIKNAVKETFLSEAEIIEVSSKNGQNIDKL
                     RQKLFELAQEVKAKKTAPEIFRIPVDRVFTIDGFGTVVTGTLTEGSIGEGDEFEIYPS
                     RIKTKVRNLQVHGKKKSKALAGQRTAVNISGIKKEKIKKGDVLAAPGSMVNTMMLDAE
                     INMFKSSKRTIKNGSRIHFHYGSAEALCKVVLLDKDELEPGETGFAQLRFDEELALKR
                     CDRFVVRFYSPVESIGGGIVLDPCPEKHKRKNKKILEALNIKAEGNDEAILLQIIKEN
                     SWKFYSKNRLRIIAGYEKDEMEKLMGELIKGGKILELENGSYIHEDFIRHIEAVAGKI
                     LKKYHSDNPIAKGMDKNEFREKLAAALYMEKGKSSEALLKLLKERGKIENRGNAVAIS
                     GFRIKYTKEQREAREQIEKYCLESGFEARDMDTFAAGRKDEKNIRQITEDMVSEGILV
                     KLNYRYYMHSKAWKKAMDLFHEHMEDNDSITLAQMRDLLNSSRKYTVLILEALDELKI
                     TRLDGDARVLRRS"
     gene            complement(7667..>8326)
                     /locus_tag="PLM92_02490"
     CDS             complement(7667..>8326)
                     /locus_tag="PLM92_02490"
                     /inference="COORDINATES: protein motif:HMM:NF015780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HPR24039.1"
                     /translation="GLPVAYDMGSGLIADLSGYGIREASVKDVIKTGVDMVFFSGDKL
                     LGGPQAGIIAGRKKYIEKMKEHPLARVVRIDKMTAAAMESVLYEYLDEENAIKKIPLL
                     KMITESEEELEKRAKKCADKIKEAVKGKKGFHVSVQSSENRVGGGTTPLTVLPGFAVV
                     ISHNEITPEKTEKNLRTNRIPVIARIHKESVNIEMRTVTEEEEDIIAAAFKSIYGGEN
                     E"
BASE COUNT         1887 a         2134 c         1474 g         2831 t
ORIGIN      
        1 tccgttgcct cgtttccgag gtcggacctt tgtctacagt ctgaaaccgc ccttttacag
       61 ggcggttttt tgtgattcca acaatctatt actgttttac ttctatgtcc cattttgtaa
      121 gatccgtatg caaaagttga tgggatttgt gtcccagagg ttttccaagg aactcttcgt
      181 atgactttag gattgccgga ttctcgtggg aaaacctgag ttcattgact ttgtcaagcc
      241 cgtagagaat cttgcctctg tcttcagcca tttcttcgcc ttcattaatt ggctgtccgc
      301 ctccgcctgc acatccgccc gggcatgcca ttatttccac aaaatcatag tttacttcac
      361 cggctcttat ggcttctata agttttcttg tatttgcaag gccgctggca acagctacac
      421 gtactgtggc cccggaaaca tcaaactccg cttctttcca gccttccatt cctcttactt
      481 tcttgaatgc atccggatcc ggatttttgc ccgtgagcag gaaataggca cttcttaatg
      541 ctgcctccat tacccctccg gtagcaccga atataactgc cgctccggaa cctatgccga
      601 atacatcatc aaattcttca ttttttagag tatcgggatt tacatgactt gccctcatca
      661 tcctgttgat ctcccttgtg gtaagaacaa tatccacatc cgaaccatat ccggaatcgt
      721 ttacctgttc tactcctctt tcatattttt tagctgtaca gggcatcaca gacaccgaaa
      781 atattttttt cggatctaca cccagatgtt ttgcaatata tgtttttatc attgctccga
      841 acatctgctg aggagatttt gccgttgaaa gctgtttcac catatcggga tactgggtct
      901 tgacaaaacg gacccaggcg ggacagcatg atgtaaacat gggccagctg taattttctt
      961 tgttcttgaa tctttcaaga aattcgcttc cctcttccat tatggtcaga tccgcagtga
     1021 aattggtatc aaagatatag tcaaatccaa gttttctgat tccgttcacc attctgccca
     1081 gggtggcctt attcttggga agtcctattc cttctcccca tgcagccctt acggcaggag
     1141 ccacctgtac tatagttact gtttcgggat cttccaaagc ttcatacatt ttgtcaatat
     1201 cgtttctttc ccttaaggct cccgtgggac aatgagtgat acactgtccg caaagagaac
     1261 agtccgcttt ctgcatgtca cttccttctg caactccaac tgtcacatgg ggccctgatc
     1321 cgtttacgtc ccatacatgc agatcctgga ttttgtcaca gatctgtaca catctcatgc
     1381 acttaataca ttttgatgcg tcccttatca gtggaaaatc tttatcccat ttgtttcttt
     1441 caggcttcca gtgataagga aattcaagta tgcccagatc attggcaacc ttctgcaact
     1501 cgcagtttcc gcttcttacg catgtggcac aaaggcagtc gtgctctgag agaagcaact
     1561 caacattagt acgccttgcc tttcttactt tatggctgtt agtaaaaatg tccatacctt
     1621 cttcagccat tgtgttgcat gatgccagca gtttatcagt gcccgcattc tcaactacac
     1681 ataccctgca ggcacctata tcatttatac cttcaagata gcagagatga ggtatgttta
     1741 ttcctatctt cccggcagcc tccatcaaag tcgttccttt tttgacagat acttttattc
     1801 cgtctatttt taaatttacc atttttgttc tctgcctcct ttgaatgatc ccattccgaa
     1861 atgatcacag cgcagacatc tggatgcttc ctgcatggct tcttccagag tcatcccttc
     1921 ctctatttct tcaaaatcct ttcgtctact ggcggaggga cgctctctca tctggatcct
     1981 tcccatagga ggtctgtttc cctgtttggc atggggaact tcaacatcta cggatatttc
     2041 gtgattgtat ccaagatatt catcaatatt tgcagccgca gtcttacctg ctgcaatgga
     2101 tcttatggct gtggccggac cggtcacaca gtcgccgcct gcaaatacac ctttgagttt
     2161 gcttactatg catgcttcat cggcgctgac cctgcctctg tctgtggcga tgcccagttt
     2221 gtcgaaggtt cccgcctcta tcttttgccc tatggcagaa atcacgaaat cagattcgta
     2281 tctttcttca gacccctttg aatcacaagg tctgggtctt ccgcttttat ccagttctcc
     2341 cggtatctga ggtttgaccc atatagcttt cacgtttccg tctttgtctg tttctattct
     2401 tgaaggggct ttgagttcaa cgacttcaca gccttcggca atagctcctt ctatctcttc
     2461 attaagagct gtcatatcgc ttcttcttct tctgtatagt atgttgacct cttttgctcc
     2521 gagccttctg gcagtccggg ccacgtccat ggccacattt cctccgccaa ttacagaaac
     2581 tgttttgcct tccagagaga tcatattccc gtccccaatg gctcttaaca tttcaacagc
     2641 agaaagaaca ccccttgaat cctcgccttt tataccaagg ctctggaatg catgggcgcc
     2701 cacagcgatg taaaccgcat cataatcttt ttttagtttc tttacttctt cggctttgga
     2761 tatatctgtt tccagttcca catccacccc cagagaaagt attatatcta tttcttcctg
     2821 tagtctttct ctcggaagcc tgtagcttgg tatgccgtat ctaagcattc cgcccaggaa
     2881 ctttcttttt tcaaatactt ttactttgtg ccccatcaaa gacaaatagt aagctgccgt
     2941 aagcccgctg gggcctccgc ctatcacagc tatttttttg cctgtacttt cagcttttgg
     3001 gggtggttct actttgccgg agttgtcaac ggcaaatctt tttagtccgc ggatattgat
     3061 gggcgcatcg atcattgtgc gcctgcacct gttttcacag gggtgttcgc acaccagtgc
     3121 acaggcaatc ggaaacggat tgtctttttt gatcaactta actgcatctt tatatcttcc
     3181 ctccagtaca agagaaacat atcccggaat atccaccctt gccgggcaaa gtcccacaca
     3241 ggggatagcc tccgaatatt ctcttatatt ggatgcacac acaccttttt caacatgaga
     3301 gatatagtct tctaaaaacc catcaagtcc tttgagcacc atattggcag cttcaaagcc
     3361 tatggcacaa tccgcactgt ctcttatact ttcggcagtg gtccttatca gacctatggt
     3421 gtctttcgta acatctttgc caccgtcaag tacatcatct atcaggtttt ccaactgttc
     3481 aagtcccact cggcatgggg tgcattttcc acaactctgg ccatgagaca gcctcaagaa
     3541 tgccgcagcc gtatcaacgg gacaaaggcc cggatggctt gcggcgaccc gtctctccat
     3601 atcttcataa agactttcaa caactatttg agcctggtct tgagacttta tgtctagtct
     3661 gctcatttta tcacatcctt taacaaatac tagactttaa agcaatagcg cttcatcttg
     3721 aattatattt tcaataacca gatatatcaa atagaaaaaa tctatgtatt tatagatacg
     3781 gatatttaag gttctacgat tatatccgct tcttttttcg caataaaaaa tcctatagcc
     3841 tcagcataag gctgtgattt ttttatgctt ctcaggagtt tttccgcttc tttttgaggc
     3901 attgagataa gaaggcctcc tgaagtctgg ggatcatgca taacatcaga catctcctca
     3961 accacatccg gagaaatttt tgctctgcat cttatgtatt ccatattggc atatgcaccg
     4021 gcaggtatta tcccttcctt tgccagttcc agagcttttt tcatacaggg tactttttta
     4081 cttttgataa ccgctgtgag tcctgatgcc tctgccattt catagcagtg ccccaaaaga
     4141 ccgaaaccgg tcacatcagt acatgcgttt atatcatatt tctttacagc ctctgcaata
     4201 aaattattca gtgaagtcat gcttcttatc atttcttctg tttcggcagg ccccaacagg
     4261 ccggcctttg ccgcagtggt cagtatgcct gtgcccaggg gctttgtcag tataagcgtg
     4321 tcaccttctt ttgctcctct gtttgtcaga agatgccggg ggtgaacgaa tccggtaact
     4381 gccagaccgt atttcggttc tctgtccttt atcgtatgcc ctcccgttat catggctccg
     4441 gcctcattta ttttatcata tccgcctttt agtatttctc cggcaatctc ctcaggcatg
     4501 tcttccgggc agcagaaaat attcattgca agcaaaggct tccctcccat tgcatatata
     4561 tcgcttacgg cattggccgc cgcgatctgc ccgaaagtat aagggtcgtc aaccacggga
     4621 ggaaaaatat caacagtctg cagcaatact ttttcttcat cgatcatata ggccgatgca
     4681 tcatctgcat tatcgaatcc cacaatcaga gcagggtcat cgggactggg caatttacgc
     4741 agaacctgcg taagcgctcc gggcgcaatt tttgccgctc aaccggaatg ctctgtcatt
     4801 tctgtaagct tcagctgctt atgattgtct tgatccatca aagtctcctc ctctccttat
     4861 ttgatactgt attcttccat aacatgtttt ttgaacatct cagcagtagg cgaaagtgtc
     4921 gtctgcctgt ggtaggtcat ataaaaactc ctttcgaatt tgaaatcttc aatatcaaaa
     4981 accaggtaat cggctttttc tgtatctctc tctaccgaaa gacctgaaat aacagatatg
     5041 ccaaggcctg cggatacagc ttttttcaca ccggtcagat tactcatcct tgctataaca
     5101 tccatttttt ttattgagat gcccatgtat gtcatggcct tttcgaaggc tttacggctt
     5161 cccgaaccca actcccgcca tacaaactca tcttccataa aatccgccat ttttatgggt
     5221 tttccatgca gttttgaata tttttcgttt ttcggtgtaa taagtacagc attatcttct
     5281 tccagcttca catataccag atcgttttcc gtcctgctac ccgtaaatcc tatttctccc
     5341 ttctgttcac ggatactgtc attcacctga ccgctgtcct cctgttcaac ataaaacttg
     5401 acattatgat atttattctt gaaggatacc ataagattag gcaatatata ctgataaggt
     5461 acgtttgatg cgaatatttc aattactcct tcaatgcttt cattataatt tttcagagaa
     5521 tatactgctt tttctcttgt acctatcatg gcgaccgcat acttgaagaa caattggccc
     5581 tgcctggtgg gtacggcctc ctttcctcct ctttctataa gctgcatgcc cagctctttc
     5641 tcaagagaac ttatatgcgc acttattgtg ggctgggtaa ggaaagaggc atccgccgcc
     5701 ttggaaaaat tatggtattt taccacattc acgaatgctt ctatctgttt gaaatccata
     5761 tcatgacctc cttaatactc ttgcatctcc atccagtctg gttatcttca gttcatccag
     5821 tgcctcaagt ataagaaccg tatacttcct ggaagaattc aaaagatctc tcatctgggc
     5881 aagagttatc gaatcattat cttccatgtg ttcatggaaa agatccatcg ccttcttcca
     5941 tgctttgcta tgcatatagt acctgtaatt cagtttcaca agtattcctt cagacaccat
     6001 gtcttctgtt atctgcctta tatttttttc gtcttttctg cccgcagcaa aagtatccat
     6061 atcacgggct tcaaatccgg attccagaca atacttttct atttgttctc ttgcttctct
     6121 ctgttctttt gtatatttga tcctgaatcc ggaaatagcc actgcatttc ctctgttttc
     6181 gatttttcct ctttctttca gaagtttcaa aagtgcttct gatgattttc ccttctccat
     6241 ataaagggca gccgccagct tttccctaaa ctcattttta tccattcctt ttgctatggg
     6301 attatcggaa tgatattttt ttaagatctt gcctgctacc gcctctatat gccggataaa
     6361 atcttcatgt atatatgagc cgttttcaag ttccaggatc ttgccgcctt ttatcagctc
     6421 tcccataagc ttttccattt catccttttc gtatccggct attattctca atcggttttt
     6481 tgaatagaat ttccagctgt tttcttttat gatctgaaga agtatcgctt catcattgcc
     6541 ttctgctttt atattcagtg cttccagtat ttttttgttt tttcttttat gtttttccgg
     6601 gcaagggtca aggacgatac cacctccaat gctttccacg ggagaataga accttacaac
     6661 gaatctgtca catcttttga gcgccagttc ttcatcgaaa cgcagctggg cgaaacccgt
     6721 ttctcccggc tccagttcat ctttatccag cagaacgacc ttgcacaaag cttctgcaga
     6781 tccgtaatga aaatgtatcc tgctcccgtt ttttatggtt cgtttgctgc ttttaaacat
     6841 atttatttct gcatcaagca tcattgtatt gaccatggaa ccgggcgcag ccaggacatc
     6901 tccttttttt attttttctt tttttatgcc tgaaatattt acagcagtcc tttgccctgc
     6961 cagtgccttt gatttctttt tgccgtggac ctgaagattc ctgacttttg tcttgatgcg
     7021 ggaaggatat atctcaaact catcaccttc acctatggac ccctccgtaa gcgtgcccgt
     7081 taccactgtt ccgaaaccat caatagtgaa gactctgtca accgggatcc tgaatatttc
     7141 gggggcagtc ttttttgctt ttacttcctg agccaactca aaaagttttt gcctgagttt
     7201 atctatgttt tgaccgtttt tcgaggagac ttctattatt tccgcttctg aaagaaatgt
     7261 ttcctttact gcatttttta tatcttcctt tacagcttct atccactctt catcagcaag
     7321 gtccgctttt gtcaaagcta taactccttt ttttattctc agggtactga gtatctcaag
     7381 atgctccatt gtctgtggca tgacaccttc atcggcagca atcaccataa gacaaatatc
     7441 tatgccgcct ataccggaaa gcatatttct tatgaatttt tcatgtccgg gcacatctat
     7501 tatccctata ttttctcctg cttctgtttt catgtttgcg aagccaagct ctattgttat
     7561 gcccctttgc tgctcttctt tgagtctgtc cgtatccgtt ccggtcaatt cctttataag
     7621 gcatgtcttg ccatggtcca catgtcctgc agtacctata atgatattat tcattttccc
     7681 ctccatatat gcttttgaat gcggcggcaa taatatcctc ttcttcttct gttacggtac
     7741 gcatttctat atttacactt tctttgtgta tccttgctat cacaggtatt ctgtttgtcc
     7801 ttagattttt ttctgttttt tcgggtgtta tctcattatg acttataact acagcaaacc
     7861 ccggaagtac cgtcaatgga gttgtaccgc ctcctaccct gttttcggaa ctctgcacag
     7921 atacatggaa tcctttcttt cccttcactg cttcttttat tttgtcagca catttttttg
     7981 ctctcttttc aagctcttct tctgattcag ttatcatttt taataagggt atctttttga
     8041 tcgcattttc ctcatccagg tattcataaa gcactgactc catggctgcc gcagtcattt
     8101 tgtctatcct gaccaccctg gcaagaggat gttctttcat tttttctatg tacttcttac
     8161 gacctgcaat tatccctgcc tgggggccgc ccagcagttt gtctccgctg aaaaaaacca
     8221 tatctacgcc ggtttttata acatccttta cacttgcctc ccggatgccg tatccggaaa
     8281 gatccgcaat aagaccgctt cccatatcgt atgccaccgg caaccc
//
LOCUS       DAONHK010000039         8185 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_6747_length_8185_cov_4.706888, whole genome shotgun sequence.
ACCESSION   DAONHK010000039 DAONHK010000000
VERSION     DAONHK010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 8185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8185
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6747_length_8185_cov_4.706888"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="PLM92_02495"
     CDS             <1..287
                     /locus_tag="PLM92_02495"
                     /inference="COORDINATES: protein motif:HMM:NF017902.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HPR24040.1"
                     /translation="SMEYREYMKAKEKISENPELEDMLKDYQQKQLAVQGKQMLGEEV
                     TSDMLQQIQNLYQIVAKDPLAAEYLQCEMRFALMMQDVYKVLGDLMGIGK"
     gene            277..981
                     /locus_tag="PLM92_02500"
     CDS             277..981
                     /locus_tag="PLM92_02500"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HPR24041.1"
                     /translation="MVNKNIKRPEELLKIMKASSIGDECFKYIIGYIREGMTEKQIAG
                     EIEETLKKLGSQGLAFPTICVSGENGADPHGSPTDKKIQRGDFLTMDFGAVVDGYCGD
                     MTRTVALGSISSEKRNVYETVLKAQENALALCRPGSRCFDIDKAARDIIEEAGYGENF
                     IHGTGHGVGREVHEEPYINKKAGDSDILAENMAITIEPGIYIPGQFGIRIEDLAIISG
                     FGIINTVKSEKKLLIL"
     gene            1027..1587
                     /gene="efp"
                     /locus_tag="PLM92_02505"
     CDS             1027..1587
                     /gene="efp"
                     /locus_tag="PLM92_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006000732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HPR24042.1"
                     /translation="MIYAGDFRKGITFEIDGEPHVVVDFQHVKPGKGAAFVRTKHKNV
                     LSGAIKEEAFNPNDKFPKAHIETKVMQYLYSDGELFYFMDKDSYEQIPIQADQVEEAM
                     KYIKENDDATIKFYKSSPFLVEAPNFVNLKVEETEPGVKGDTATNVTKAAKVETGAVI
                     QVPIFIEEGELIQIDTRTGEYLGRSR"
     gene            1677..2723
                     /gene="mltG"
                     /locus_tag="PLM92_02510"
     CDS             1677..2723
                     /gene="mltG"
                     /locus_tag="PLM92_02510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HPR24043.1"
                     /translation="MVKKLRIKNKRTFTVALSVALLLVLIIAAGIILAVLGRPVDPSS
                     KDTVIVNIKEGSGIAQIASVLHENSLIRSETVFKIRSKISFYDGKYKAGDYALSKAMS
                     MKEIVQTIISGRSAGQAFQTIEGETIKQTAKRLEKRGIVSEKEFLKEVAEGDFDYRFM
                     DQLPAGETRLEGFLFPNMYEVSLNADAHEVIDTMLAEFNSIVSDDYYKKAKKMGYSMY
                     EMITLASIVEREAVENQDRAKIASVLYNRLEKNMKLQCCSTVQYILGEQKPVLSNADT
                     RIESPYNTYIITGLPQGPICSPGKASIDSVFEPADTDYLYFVLKSKDSTSHNFSKNYE
                     EFMKDKNKYAESFK"
     gene            2726..3391
                     /locus_tag="PLM92_02515"
     CDS             2726..3391
                     /locus_tag="PLM92_02515"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HPR24044.1"
                     /translation="MNITNEHIIEYTDNFYKPVSEKLRELRNVEEKGGIPIIRKDTES
                     LIISLLYILKPQRILEIGTGGGYSATVMATVCPKSTILTVEKDPRAVRRASSNFDEAD
                     LSHRVSILQGDGRKVISDLTEKRNKKELKPFDFIFIDAAKSHYMDFFNESIKVSHAGT
                     LILCDNILLGGITADERYLKSRRDRTSMNRMKEFLEYIKETDKGDTFVLPMGDGVSLT
                     YVK"
     gene            3381..4631
                     /locus_tag="PLM92_02520"
     CDS             3381..4631
                     /locus_tag="PLM92_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005586040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HPR24045.1"
                     /translation="MSNKNFKKIELLAPAGDIEKLITAVDYGADAVYFGGEDFSLRAS
                     AGNFSIEEMKKGVDHTHERGRKCYLTVNAFSHNEDIKPIENYFSEIKKSDIDMDAFIV
                     SDPGVIEILKKIIPDAVIHLSTQANTTNYNAAGFWHRAGIKRAVVARELALKEIKEIR
                     ENIPADMELEAFVHGAMCISYSGRCLLSNFMAGRDANRGMCAHPCRWKYSLTEEERPG
                     EYYPVEEDKRGTYIMNSKDLCMIEHIPELIDSGLDSIKIEGRMKSIFYTAVVTSVYRK
                     AIDAYYKDPENYRYDPAWMTELEKVSHREFTTGFYFSKPGSSDQNYKTSSYIREYSFT
                     GLVKEYDADTKMALVEQRNKMCRGEEIEIFGPETDFFKQKLEVLLDEWGDSLDSAPHP
                     QQLIKIKTEQPVSAGFIIRKRKEQ"
     gene            4634..5998
                     /locus_tag="PLM92_02525"
     CDS             4634..5998
                     /locus_tag="PLM92_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017416491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HPR24046.1"
                     /translation="METNPDPGIPFAVQLLFLLFLILVNAFFAMAEMALVSSNKNLIS
                     GIAREGNKKAQIVEKLLEEPNKFLSTVQVIITLAGFLASAAAAVGISDDLEAYFNNNG
                     WPYSEQIAVFLVTIVLAYVTLVLGELYPKRIAMQHSEKIAMFVARPLKVMSVITRPFV
                     WLLSVSVNFFLKITGQKTKTEDEGFSEEEVMSMLETGQEKGALKEEGKRMINSIFAFD
                     DKLAYEIMTPRTDVFMINIKDPTEEYIDELMELRYSRIPVYDDDNDNIKGIVHIKDYL
                     IKAREEGFENVDIKAILREPYFVPETKNIDTLFFELQKTKQHIALLIDEYGGFSGIVT
                     MEDIIEEVMGEIDDEYDMEEPKIEKIDDENWQIDGTVYLDDINEELGTDFKSENSETI
                     SGLLIEKLGEIPEDSDHENITVDIENYSFIIESVKDRRIEKVRLKIMPTFEADREEKR
                     QEDE"
     gene            5991..7304
                     /gene="mtaB"
                     /locus_tag="PLM92_02530"
     CDS             5991..7304
                     /gene="mtaB"
                     /locus_tag="PLM92_02530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HPR24047.1"
                     /translation="MSKKKAVFYTLGCKVNQYETEAIKEAFRKAGYDIVKEDEPADVC
                     VINTCTVTGLADRKSRQFIRRSKKNNPDAVVAVTGCYAQISPREVSSIEEVSVIAGTN
                     EKTNLPEYVRRFTETGEKQIKCLKTEDISDFEEAVDAISTGERTREYIKIQEGCDRFC
                     SYCIIPYARGRIRSRNLGRIREEAEALISAGTGEIVITGINTALYGGENGEADIEPVI
                     KMINEIEGDFRIRLSSLEPTVIDAAYVKRLFKYEKLCHHLHLSAQSGSDRVLAAMNRK
                     YTSKDYMDIVETLRSFDPYYGITTDMIAGFPGETEEDIEDSISMIKKAGFSKVHIFKY
                     SPRKGTAAALMSDQIPPHIKTHRSERLSMAGEEEGKKFIEKNLGLVRRILAEEYKNRM
                     VFGKSGNYINTCFQCEEKKAYSLLNSFVEVRMIKGYKGGVYSEII"
     gene            7322..7885
                     /locus_tag="PLM92_02535"
     CDS             7322..7885
                     /locus_tag="PLM92_02535"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HPR24048.1"
                     /translation="MKKRTIILSAFLILLVGVFAGCGNAEETDINKEINKDYEKYFAE
                     FSAEDIYGKTVTQNEIRDNKYTLITVWGTYCSPCIDELPDLQKLYDKYRGDGINVLGV
                     VIDIQNDDGSLNEDQNSYAQEITDKQGVEFVNMAVPPGREGLFSEIAVTPTAFFIDGE
                     GNVIGDFYEGAYSYEEWSDMVEKLLEE"
     gene            7893..8006
                     /locus_tag="PLM92_02540"
     CDS             7893..8006
                     /locus_tag="PLM92_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CD1871A family CXXC motif-containing protein"
                     /protein_id="HPR24049.1"
                     /translation="MVLLAGIILICIGISRGEVAVVFTKAVNVCLECIGIG"
     gene            7999..>8185
                     /locus_tag="PLM92_02545"
     CDS             7999..>8185
                     /locus_tag="PLM92_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005949030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPR24050.1"
                     /translation="MDRIRTVVQIAFVAVTNGYYRGFAEGTIFKGDTKNLCLPGLNCY
                     SCPGALGSCPIGSLQAVS"
BASE COUNT         2894 a         1382 c         1912 g         1997 t
ORIGIN      
        1 aatcgatgga atacagggaa tacatgaaag caaaagaaaa gatatcggag aatcccgagc
       61 ttgaagatat gctgaaggac tatcaacaaa aacagctggc tgtacagggc aagcaaatgc
      121 ttggcgaaga agtcaccagt gatatgctgc agcagataca gaatctttat cagatagtgg
      181 caaaggatcc tctggcagca gaatatctgc agtgtgagat gcgttttgct ttaatgatgc
      241 aggacgtata caaggtatta ggagacctga tgggaattgg taaataaaaa tataaaaagg
      301 cccgaggaac tgctgaaaat aatgaaagct tcctccatag gcgatgagtg tttcaagtat
      361 atcatcggct acataaggga aggaatgaca gaaaagcaga tcgccggtga aattgaagaa
      421 acattaaaaa agcttggctc ccagggactg gcttttccga ctatttgtgt ttcgggagaa
      481 aacggagccg atcctcacgg aagtcccaca gataaaaaaa tacagcgggg agattttctc
      541 acaatggatt tcggagcggt ggtagacggc tactgcggcg atatgacaag aacagtcgct
      601 ttgggcagta taagcagtga aaaaagaaat gtgtatgaaa cagtccttaa ggctcaggaa
      661 aatgctcttg ctttatgcag accagggagc aggtgctttg atatagataa agcggcaaga
      721 gacataatag aagaagccgg atacggagag aactttatac acggaacagg ccatggtgtc
      781 ggcagagaag tgcacgaaga accttatata aacaaaaaag caggtgatag cgacatactt
      841 gccgagaata tggccataac catcgaaccg ggaatatata tacccggaca gttcggtata
      901 cgcatcgaag acttggcaat aataagcgga tttggtataa ttaataccgt taaatccgag
      961 aaaaagctat tgatactttg attttgaatt atcgatattt gattttgaac gaaacggaga
     1021 agaaaaatga tttacgcagg agatttcaga aaaggtatta catttgaaat agacggagag
     1081 ccccatgttg tagttgattt ccagcatgta aaacccggaa agggagcagc ttttgtgcgg
     1141 acaaaacata aaaacgtact ttctggagcc ataaaagaag aagctttcaa tccaaacgat
     1201 aaatttccaa aagcccatat agaaacaaag gtcatgcagt atctttattc agatggagaa
     1261 cttttttatt tcatggacaa ggattcttat gaacagatac ccattcaggc ggatcaggtg
     1321 gaagaagcca tgaagtacat aaaagaaaat gacgatgcaa cgattaagtt ttataaatca
     1381 tccccgttcc ttgtggaagc accgaatttc gtcaatttga aggtggaaga gacagaaccc
     1441 ggagtaaaag gagacacggc caccaatgtg acgaaagcgg caaaggtgga aacgggggct
     1501 gtcatacagg tgcccatttt tatcgaagaa ggagaactca tacagataga cacaaggacc
     1561 ggagaatatc tgggccggtc aagataaaaa gtcgcaaggg acgtttgttt caggcgtcct
     1621 ttattatata tgaggcaaaa ctataataaa tatatttgtg aggaaacaga gaatatatgg
     1681 taaagaagct cagaataaaa aataaaagga cttttacggt tgctctttct gttgccttac
     1741 tgctggtttt gataattgca gcaggaataa tactggcagt tttaggaaga cctgtcgatc
     1801 catcttccaa agatacggta atagtaaaca taaaagaggg tagcggaata gcacagatag
     1861 cttctgtcct tcatgaaaac agccttataa gaagtgagac ggtatttaag ataagatcaa
     1921 aaataagctt ttacgatggt aagtacaagg ccggggacta tgcactttca aaggcaatgt
     1981 caatgaaaga aatagtacag acaataatct cagggcggtc cgcaggacag gcatttcaga
     2041 ccattgaggg agaaacaata aaacagactg caaaaaggct tgaaaagcgg gggatagtgt
     2101 cagaaaaaga attcctgaag gaggtagccg aaggagattt tgattacaga ttcatggatc
     2161 agctaccggc gggagaaacc agacttgaag gatttctttt cccaaatatg tatgaagtat
     2221 cactgaatgc cgatgcgcac gaagtcatag atacgatgct ggcagaattt aacagcattg
     2281 tatcagacga ttattataaa aaagcaaaaa aaatgggtta cagtatgtat gaaatgataa
     2341 cgctggcatc aatagtggaa agagaagcag tggaaaacca agaccgggcg aaaatagcaa
     2401 gcgtgcttta taacagactc gaaaaaaaca tgaaattgca gtgctgttca acggtccagt
     2461 atatacttgg agaacaaaag ccggtactca gcaatgctga tacaaggata gaatctccct
     2521 acaacacata tatcattacc ggacttcccc aaggacccat atgctctccc ggcaaagcct
     2581 caattgattc tgtttttgag ccggctgata cggattacct ttactttgta ttgaaatcaa
     2641 aggattccac atcgcataat ttttcaaaaa actatgaaga gttcatgaaa gataaaaaca
     2701 agtacgctga atcttttaaa taggcatgaa tattacaaac gagcatataa tcgaatatac
     2761 ggataacttc tataagcctg tcagtgaaaa gctcagggaa cttagaaatg tcgaagaaaa
     2821 aggaggcatc cccattataa ggaaggatac cgaaagtctc atcatcagcc ttctttatat
     2881 tctgaaaccc caaaggatac ttgagatagg taccggagga ggttattccg ctacagttat
     2941 ggcaacggtc tgcccgaaaa gcaccatatt gaccgtcgaa aaagatccga gggctgttag
     3001 gagggcatct tctaattttg atgaggccga tctttcacac agagtcagca tacttcaagg
     3061 tgacggccga aaagtcatct cagatcttac ggagaaaaga aacaaaaaag aactcaagcc
     3121 atttgacttc atttttattg atgcagcaaa aagtcattat atggattttt ttaatgaaag
     3181 cataaaagta tctcatgccg gcactcttat tctatgtgac aatattttgc tgggcggtat
     3241 aactgcagat gagaggtatc tgaaatcaag gagagacaga acaagtatga acagaatgaa
     3301 agaatttctt gaatatataa aagaaaccga taagggagat acattcgttc tgcccatggg
     3361 agacggagtg agccttacat atgtcaaata aaaacttcaa aaagatagaa ctgctggctc
     3421 ctgcgggaga tattgaaaaa cttattacgg ctgttgacta tggagcggat gccgtatatt
     3481 tcggaggaga agacttcagc ctcagggcat cagcaggaaa tttttcgatc gaagaaatga
     3541 aaaagggagt ggatcatacc catgaaaggg gcaggaaatg ttaccttaca gttaatgctt
     3601 tctcccataa cgaagacata aaacctatcg aaaattattt cagtgaaatc aaaaaatcag
     3661 atatagatat ggatgcgttt attgtttctg atccgggagt tattgaaata ctcaaaaaaa
     3721 ttatccctga tgctgttata catttgagta ctcaggcaaa tacgactaat tataatgcag
     3781 caggtttctg gcatcgggca ggcataaaaa gagccgtcgt ggcaagggaa ctggccttga
     3841 aggaaataaa agaaataaga gaaaacatac ctgccgatat ggaacttgaa gcatttgtgc
     3901 acggagctat gtgtatttca tattcgggca ggtgtctttt aagcaatttt atggctggaa
     3961 gagatgcaaa cagaggtatg tgtgcccatc catgcagatg gaagtattcc ctgacagaag
     4021 aagaaagacc gggagaatat tatcctgttg aagaagacaa aagaggtaca tacataatga
     4081 attcaaagga tctgtgtatg atagagcata taccggaatt gatcgattcc ggtcttgatt
     4141 ccataaaaat cgaaggccgc atgaaaagca tattttacac agcagttgta acatccgtat
     4201 acagaaaagc aatagatgcg tattacaaag atcctgaaaa ctacagatat gatcccgcct
     4261 ggatgacgga gcttgaaaaa gtcagtcaca gagaattcac cacgggtttt tatttttcta
     4321 aaccgggcag cagtgatcag aattataaaa cgagcagcta tataagagaa tactctttta
     4381 caggccttgt caaagagtat gatgcagata caaaaatggc tttggtggaa cagagaaaca
     4441 agatgtgcag gggagaagaa atagagattt ttggtcctga aacagatttt tttaagcaga
     4501 aactggaagt attgctggat gaatggggag attcgctgga ttctgccccg catccgcaac
     4561 agctgataaa aataaaaacg gaacagccgg tcagcgccgg atttattatc agaaaaagga
     4621 aggagcaata ggaatggaaa cgaaccctga tcccgggata ccttttgcgg tgcagttatt
     4681 attcttatta tttttgatat tagtcaatgc tttttttgcc atggcagaaa tggctctggt
     4741 gtcatcaaac aaaaacctta tatcaggcat tgccagggag ggaaataaaa aagcacaaat
     4801 agttgaaaag cttttggaag aacccaataa atttctttct actgttcagg tcataataac
     4861 tcttgccgga tttcttgcca gtgcagcagc ggcagtgggc atttcagatg atcttgaagc
     4921 atactttaat aataacggat ggccttacag cgaacagata gccgttttct tagttaccat
     4981 agtattagct tatgtaacac tcgttctcgg agaactttat ccaaagcgga tagctatgca
     5041 acattcagaa aagatagcta tgtttgtagc aagaccccta aaggtaatgt ctgttataac
     5101 aagacccttt gtatggttgc tttcggtttc ggtgaatttc tttttaaaga tcacaggaca
     5161 aaagacaaag actgaggacg aaggattctc tgaagaagaa gtaatgtcaa tgcttgaaac
     5221 gggacaggaa aaaggagcat tgaaagaaga gggcaaaagg atgataaaca gcatatttgc
     5281 cttcgatgat aaactggcat acgaaataat gacaccgagg acagatgttt ttatgataaa
     5341 tataaaggat cccacagaag aatatattga tgagctgatg gaacttcggt attccaggat
     5401 acctgtttat gatgatgaca acgataatat aaaaggtata gtccatataa aggattatct
     5461 tataaaggca agggaagaag gctttgaaaa tgttgatatc aaagcaatac tcagagaacc
     5521 gtattttgtt cccgaaacaa aaaatataga cacgcttttc ttcgaactcc agaaaacaaa
     5581 acagcatata gctctgctta ttgatgaata cggaggattt tcaggtatcg ttaccatgga
     5641 agatataatt gaagaagtta tgggagaaat agatgatgaa tacgacatgg aagaacccaa
     5701 aatcgaaaaa atagatgatg aaaactggca aatagacgga actgtctatc tggatgatat
     5761 aaatgaagaa ctgggaactg atttcaagtc tgagaacagt gaaaccataa gtggtctttt
     5821 gatagaaaag cttggagaaa taccggaaga cagcgatcat gagaatatta ccgtggatat
     5881 tgagaattac agtttcatta ttgaatcggt caaggacaga aggatagaaa aagtaaggct
     5941 taagataatg cctacttttg aggctgacag ggaagaaaaa aggcaggaag atgagtaaaa
     6001 agaaagctgt gttttataca cttggctgca aggtcaatca gtatgaaaca gaagcaataa
     6061 aagaagcatt tagaaaagcc ggctatgata tcgtaaaaga agatgaaccg gcggatgttt
     6121 gtgtaataaa tacatgcaca gtgacaggac tggcggacag aaaatcaagg cagtttataa
     6181 ggcgttccaa aaaaaacaat cccgatgcag ttgttgcagt aaccggatgt tatgcacaga
     6241 tcagtccccg ggaagtatca tccatagaag aagtttctgt tattgcaggt accaacgaaa
     6301 agacaaatct gccggaatat gtgcggcgct ttacagaaac cggagaaaag cagataaaat
     6361 gcctgaaaac agaagatatc agtgattttg aagaagcagt cgatgccatt tcaacgggcg
     6421 agaggaccag agaatatatt aagatccagg aaggctgcga caggttctgt tcctactgca
     6481 tcatacctta tgcgagagga agaataagaa gcagaaacct tggcaggata agagaagaag
     6541 cagaagctct catatctgca ggaacaggag agatagtgat caccggtata aacaccgccc
     6601 tttacggtgg tgaaaacgga gaggctgaca tagaacctgt cataaagatg ataaacgaaa
     6661 tagaaggcga cttcagaata aggctcagtt ctctggagcc cacagttatc gatgccgcat
     6721 atgtaaaaag acttttcaaa tatgaaaaac tctgtcacca ccttcatctt tctgctcaaa
     6781 gcggtagcga cagagtgctt gccgccatga acaggaaata tacaagtaaa gattatatgg
     6841 atatagtaga aaccttgaga agttttgatc cttattacgg tatcaccacc gacatgatcg
     6901 ccggatttcc cggagaaact gaagaagata tcgaagacag catttccatg ataaaaaaag
     6961 caggattttc caaagtgcat attttcaagt attctccaag aaagggaacg gcagcagctt
     7021 tgatgtccga tcaaatccca cctcacataa aaacccacag aagtgaaaga ctttccatgg
     7081 caggcgaaga agagggaaaa aagttcattg aaaaaaatct ggggctggtc cggcgcatac
     7141 tggctgaaga gtataaaaac aggatggtat tcggcaagag cggcaattat ataaatacat
     7201 gttttcagtg tgaagaaaaa aaagcgtaca gtcttctgaa cagtttcgtt gaagtaagga
     7261 tgattaaagg atataaaggc ggggtatact cagagataat ataataacag gaggcgacca
     7321 aatgaaaaaa agaacaatta tattatccgc ttttttgata cttctcgttg gtgtttttgc
     7381 cggatgcgga aatgcagagg aaacagatat caacaaagaa atcaataaag attatgagaa
     7441 gtattttgct gaattttcgg ctgaagacat atatggcaaa acagtgacac aaaacgagat
     7501 cagagataac aagtatactc ttataactgt atggggcacc tactgcagcc cttgcattga
     7561 tgaacttccg gatttgcaga aactttatga caagtacaga ggggacggaa taaatgttct
     7621 gggtgttgta atcgatatac aaaatgatga cggaagtctt aatgaagatc agaattccta
     7681 tgcacaggag ataacagaca aacagggtgt ggaatttgta aatatggcgg tgccgccggg
     7741 cagagaaggc cttttttctg aaatagcagt gacacctaca gcattcttta ttgacggcga
     7801 aggcaatgtt atcggagatt tctatgaggg ggcatacagt tacgaagaat ggagtgatat
     7861 ggttgaaaaa cttcttgaag agtaaagcat tcatagttct tctggcaggt attatactta
     7921 tatgcatagg gatatcaaga ggcgaagtgg cagttgtttt cacaaaggct gtaaatgtat
     7981 gtttggagtg tataggtatt ggatagaata aggactgtgg tccagatagc ctttgtggcg
     8041 gtcacaaacg gatattacag gggttttgcc gaggggacca tattcaaagg agacacaaag
     8101 aatctttgtc ttccgggtct gaactgctat tcctgtcccg gagctcttgg atcatgtccc
     8161 atcggctctc ttcaggctgt ttcca
//
LOCUS       DAONHK010000040         8133 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_6806_length_8133_cov_4.637905, whole genome shotgun sequence.
ACCESSION   DAONHK010000040 DAONHK010000000
VERSION     DAONHK010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 8133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8133
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6806_length_8133_cov_4.637905"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(123..635)
                     /gene="atpF"
                     /locus_tag="PLM92_02550"
     CDS             complement(123..635)
                     /gene="atpF"
                     /locus_tag="PLM92_02550"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HPR24051.1"
                     /translation="MEFAPLISFNWTLLMQLVTVFVLYIILRKFFFEKVHDFVQKRES
                     TVKEAFDSAEATNRKADEKLEAYSIKIAQIESEGREIIRESKVKADEKAKEIIDEANA
                     KANEIMMRAEKEIKRQKEKALVDMKEEVGMLAVMAAEKIMEKDLEKTDEQKQIVDKII
                     KGAGISEWQN"
     gene            complement(675..938)
                     /gene="atpE"
                     /locus_tag="PLM92_02555"
     CDS             complement(675..938)
                     /gene="atpE"
                     /locus_tag="PLM92_02555"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006784602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HPR24052.1"
                     /translation="MSSQITPEALIYAFSALGAAVSIGFAALGIGIGQGLAASRAVEG
                     VGRQPEAKGDLLQVMLVGQAVTETTGIFAFIVSIVLLFANPFA"
     gene            complement(976..1686)
                     /gene="atpB"
                     /locus_tag="PLM92_02560"
     CDS             complement(976..1686)
                     /gene="atpB"
                     /locus_tag="PLM92_02560"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HPR24053.1"
                     /translation="MDIGPQIVIKFDNGIFITNTVCFGIIVAVILIVLALWSTRRMKK
                     VPDGKQVIAEVVVEGVYNMVKATMGPKAINYAPYIGTLFFFVLLSNMLGLFGQRPVTA
                     DVNTTFALAAITFVLIQRQALKSYGLIGKMKEMAQPYPFMFPLKIIEQLSFPISLSFR
                     LFGNILGGVIVIEMLLGGLATASEALHLPIPLLDAVIPLPANFFFDVFEPVLQAFIFT
                     MLTMVFITMEIATRDEEH"
     gene            complement(1705..2121)
                     /locus_tag="PLM92_02565"
     CDS             complement(1705..2121)
                     /locus_tag="PLM92_02565"
                     /inference="COORDINATES: protein motif:HMM:NF015834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HPR24054.1"
                     /translation="MDEFAKFKNRILLYGLVAAGLFELISLIVLGFSTRFVYGLLIGT
                     CVAVVNFNIMIFCAKRVLEKGKPYWSTLGYVIRFLIYGVVLYMTFRSSYIVGIGCGAG
                     FLTIQTSIFYIHILRPKIFRKKKDGETTDIKDQPKS"
     gene            complement(2365..2832)
                     /locus_tag="PLM92_02570"
     CDS             complement(2365..2832)
                     /locus_tag="PLM92_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011024654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HPR24055.1"
                     /translation="MARTIIKERPDWDHYFMKMAELAATRSTCLRRQVGAAIVMDKHV
                     ISTGYNGAPKGVPHCEERGACMREEMKIPSGERHELCRALHAEQNAIIQAATYPQSIE
                     GATIYITHQPCLICAKMIINAGIRRIVVREGYPDEMATEMLAEAGLKIVMLDK"
     gene            complement(2898..3638)
                     /gene="truA"
                     /locus_tag="PLM92_02575"
     CDS             complement(2898..3638)
                     /gene="truA"
                     /locus_tag="PLM92_02575"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015908110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HPR24056.1"
                     /translation="MYKNILLKIEYDGTDFCGWQIQPDVPTVQGQIEEAIYFLSGTRT
                     EIKGTSRTDAGVHAMGQMASFEGDIKIPTERLAYVLNNHLPGSIRIKEAIEKPLGFHA
                     RFDAKGKKYIYRIMNCSQRDPFKRNFYYFVEKPLDLPSMQKAAAYIEGKNDFKCFQAA
                     GSTEKETTVRTVKNLSIKKESDEITIEITGDGFLYNMVRIIAGTLVDVGHGKISPLDM
                     PLIIKSKDRRKAGHTAPPQGLYLAEVFY"
     gene            complement(3642..4724)
                     /locus_tag="PLM92_02580"
     CDS             complement(3642..4724)
                     /locus_tag="PLM92_02580"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPR24057.1"
                     /translation="MRYLKGFIRDSKIYFSNCKKDIKNDNIFLLRRNSISALMGLMMY
                     LYVVLKDFTSVELVIAYNFFVVVFGVFCIFCLLYFQKREGSFFMVQALCFVFSVMVLS
                     FFIFISVFPFPDLPAIFLFLGYTFLPLVFIMPMKIYIFLLLAFQGIFCSFVFMFKDSG
                     TFAFDMLQSSIACGAGILMAFVVLDLRLRANKTKLQLQRMNAMDPLTGIFNKATTTQN
                     CRFYLDYYSREEKCSLMVIDIDDFKKVNDFMGHQTGDHILSSVGEILRDVSRKSDIVG
                     RIGGDEFLFLMKNTSDKDILRYRATQICEKISGISIDNEKNVSCSIGVAVSPEKGTDF
                     KTLFARADKAMYDAKRKGKNTFSIFD"
     gene            complement(4729..5883)
                     /locus_tag="PLM92_02585"
     CDS             complement(4729..5883)
                     /locus_tag="PLM92_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006598154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HPR24058.1"
                     /translation="MDYYKASLIMHEKNKGKMATAGKVRVKTKEDLSTAYTPGVAEPC
                     RKIKECPEDIYRYTNKSNTVAVVSDGSAVLGLGNIGAAASVPVMDGKALLFREFAGID
                     AFPVCIKTQDPDEIVKIVKNISSTFGGINLEDISAPRCFEIEEKLQGELEIPVFHDDQ
                     HGTAVVVSAALINAAKLTGRKLWEIKAVINGAGAAGTAIAKMLMDMGIEDIVVCDSHG
                     IIDKKRNDLNETKKKLASITNKKDLSGELAEALAGRDLFIGVSSGGALTQKMVKTMAG
                     SPMIFAMANPEPEIMPEEAKKAGAAVIATGRSDFPNQINNVLIFPGIFKGALSVRAKR
                     ITEKMKKAAAYALAGMINEEDISEENIIPSAFMPGVAEKVAEAVAKAWEG"
     gene            complement(5895..7544)
                     /locus_tag="PLM92_02590"
     CDS             complement(5895..7544)
                     /locus_tag="PLM92_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011591700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HPR24059.1"
                     /translation="MSEKENITSNFIHNIIDADNETGKYGGRVYTRFPPEPNGYLHIG
                     HAKSICLNFSTAGKYEGKCNLRYDDTNPVKEDEEYIDSIEEDVKWLGFQWDKKLWASD
                     YFEKMYEAALELIKKEKAFVCDLDAEEIREYRGTLKDPGKESPYRKRSKEENLKLFEE
                     MKAGKYSDGEKVLRAKIDMASPNINMRDPVIYRIAHVPHHNTKNKWCIYPMYDFAHPI
                     EDAIEGITHSICTLEFEDHRPLYDWFLAEVGWWQTPPRQIEFARLNITKTVMSKRLLK
                     SLVENGDVEGWDDPRMPTIAGLRRRGYTPAAIRDFCERIGVAKSNSTVDIALLEHCVR
                     EDLKKTTETRNVVENPLKVIITNYPEDKTEEMEIENNANVPEMGTRKVTFSRELYVDG
                     EDFAEVPVKKYFRLFPGNEVRFKGAYFITCNDVIKNPDGTIKELHCTYDPATRSGSGF
                     DGRKVKGTIHWVDAKNALKIKIRNYDHLMTEGDDGSKKINADSLREATAYAEASVANA
                     MPCERYQFFRHGYYIADKILTSEKQKVFNRIVDLKSSFKIK"
     gene            7656..>8133
                     /locus_tag="PLM92_02595"
     CDS             7656..>8133
                     /locus_tag="PLM92_02595"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HPR24060.1"
                     /translation="MTLGYNKDGFLKLPTPLERLEGISRDTGVDFWIKRDDLTDPGMG
                     GNKLRKLEYFIHDALDKKSTVLITTGAPQTNHGRLTAAVAAKYGMKCAIVSIADHSGE
                     ISGNILLDRIMGAEVFIKRPEPGISDGSLLSETLAKITRNYENQGEKVYFVPMGGSS"
BASE COUNT         2115 a         1867 c         1485 g         2666 t
ORIGIN      
        1 gataataaca ggagcagtta tctcagaatg gcagaactga tcattgatat gacatatgga
       61 agtgctctgt ttcaggtggc gcaggatctg aaacagagca cttccatatg tcatatcaat
      121 ggtcagttct gccattctga gatacctgct ccttttatta tcttatcgac gatctgcttc
      181 tgctcgtcgg ttttctctaa atctttttcc attattttct cggcagccat tactgccagc
      241 atcccgactt cttctttcat atccacaaga gctttttctt tctgtctttt gatttctttt
      301 tcggctctca tcatgatctc atttgctttg gcatttgcct catcgattat ttcttttgcc
      361 ttctcatctg ctttcacttt ggattccctt atgatctccc tgccttcgct ttctatctga
      421 gctatcttta tactgtaagc ctccagtttt tcgtcagctt ttctgttggt ggcttctgcc
      481 gaatcaaaag cttccttgac agtgctttct ctcttctgca caaaatcatg gactttctca
      541 aaaaagaatt ttctcagtat aatataaagg acaaaaactg ttacgagctg catcagcaat
      601 gtccagttga aactgataag cggtgcaaat tccataggct gtctcctccc ttctttggct
      661 ttttattcgt tttctcaggc aaaaggattt gcgaaaagta aaacgatgga tacgataaat
      721 gcaaatatac ctgtagtctc cgttactgcc tgtcctacaa gcattacctg aagcagatca
      781 cccttggctt cgggttgtct tccaacgcct tctacagctc tgctggctgc aagaccctgg
      841 cctataccta taccaagtgc ggcaaagccg atacttactg ctgctcccaa tgcggaaaag
      901 gcataaatca gagcttccgg tgttatttgt gatgacataa tatttacctc ctaataataa
      961 ttgattgctt gtatgtcaat gttcttcatc gcgagtcgct atttccatcg tgatgaatac
     1021 catagtcaac attgtaaata taaaggcctg aagtacaggc tcaaaaacat caaagaaaaa
     1081 attcgccggc aaaggtatta ctgcgtccaa aagaggtatt ggcaaatgca gggcttcact
     1141 ggcggtggcc agtcccccaa gaagcatttc tataactatc acaccgccaa ggatgttacc
     1201 gaaaagtcgg aatgaaagag aaatggggaa ggaaagctgc tctattatct taagagggaa
     1261 cataaaggga tacggctgag ccatttcctt catttttcct attaatccat agctcttaag
     1321 agcctgtctc tgtatcagta caaaagttat tgctgccagg gcaaatgttg tgtttacatc
     1381 ggcggtaacc ggtctctgtc cgaaaaggcc cagcatattg ctgagaagca caaagaaaaa
     1441 aagtgttccg atatagggtg cataatttat tgcttttggt cccattgttg ctttcaccat
     1501 attatataca ccttcaacga caacttctgc tatgacttgt ttgccgtccg gcactttttt
     1561 cattcttctt gtggaccaca gtgccaggac aatcaaaata acggcaacaa ttataccaaa
     1621 acaaacagta tttgttataa aaatgccgtt atcaaattta ataactatct gagggcctat
     1681 atccacgccc ttacctcctt gccatcagct ctttggctga tcttttatat ctgtggtttc
     1741 accatctttc tttttcctga aaatcttcgg tctcaggatg tgaatataaa atatcgaagt
     1801 ctgtattgtc aggaatccgg ctccacagcc tatccctacg atgtatgagg atctgaacgt
     1861 catataaaga acgaccccgt aaatcaaaaa tcttattaca taacccagag tggaccagta
     1921 gggttttcct ttttcaagta cccgttttgc gcagaaaatc attatattaa agttaactac
     1981 ggccacacat gtcccgatca aaagtccgta aacaaatctt gtgctgaacc ccaaaacgat
     2041 cagagatatc agttcaaaaa gacctgctgc gaccagtccg tataacaata tcctgttctt
     2101 gaatttcgca aactcgtcca tgatatactc tattatacgc cgaaaaaaat aaaagtaaac
     2161 ctttgttaaa atttacacga atcctcttga aacactcaaa ttctaagatt tcaagctttg
     2221 tttataaaaa aaacaaatca gagcattccc ataaggcaat ttcattgtgg ccgttttaaa
     2281 gggtcacctt tgctctgatc aaatgttttt taccttttgc ttttccggca aaaacagaaa
     2341 tcctgtttca gatatgatta tcatttattt atcaagcatc actattttaa gacctgcctc
     2401 tgcaagcatc tctgttgcca tttcatcagg gtatccttct cttacgacta ttctcctgat
     2461 gcctgcgttt attatcatct ttgcgcatat gaggcagggc tgatgcgtta tgtatattgt
     2521 ggcaccttcg atactctggg gatatgtggc tgcctgtatt attgcgttct gttctgcatg
     2581 cagggcccgg caaagttcgt gtctttctcc cgagggtatt ttcatttctt ccctcatgca
     2641 tgcccctcgt tcttcacagt gaggaacacc cttgggagct ccgttgtaac ctgtcgatat
     2701 aacatgtttg tccattacta tggccgcccc cacctgcctt ctcagacaag tggatcttgt
     2761 ggctgccagc tctgccattt tcataaaata gtggtcccag tcgggtcttt ctttaattat
     2821 ggttcttgcc attacattct cctttgataa taatatcggc tcaaaaaaat cgtatcatac
     2881 cttatatcaa atatcaatca gtaaaaaacc tctgccagat aaaggccctg gggaggggcc
     2941 gtatgtcccg cctttcttct gtcctttgat tttattatca ggggcatgtc caaaggagat
     3001 attttcccgt gccccacatc caccagcgtc ccggctatta tcctgaccat attataaagg
     3061 aacccgtccc ccgtgatctc aattgtgatt tcatctgatt ctttctttat ggaaagattt
     3121 tttactgtcc ttacggtagt ctctttttca gtactcccgg cagcctgaaa acatttgaaa
     3181 tcatttttgc cctctatata tgctgccgct ttttgcattg agggaaggtc aagcggcttt
     3241 tctacaaaat aataaaaatt tcttttgaaa ggatctcttt gggaacagtt cattatccta
     3301 taaatatact ttttcccttt tgcatcaaaa cgggcatgaa aacccagagg tttttcaatg
     3361 gcttctttta ttcttattga tccgggcaaa tgattgttga gaacatacgc cagtctttca
     3421 gtgggtattt taatgtcacc ttcaaagctg gccatctgcc ccatggcgtg aactcccgca
     3481 tccgtcctgc tggtcccttt tatttctgtt cttgttccgg ataagaaata tattgcttct
     3541 tcaatctgtc cctgaactgt cggtacatcg ggttgtattt gccatccgca aaaatcagtt
     3601 ccatcatatt caattttaag aagaatgttt ttgtacattt tttaatcgaa aatactgaat
     3661 gtatttttcc cttttctttt tgcatcgtac atagctttgt ctgcccgggc aaagagagtt
     3721 ttgaaatctg tgcccttttc aggacttaca gcgacaccta tactacagct gacatttttt
     3781 tcgttatcaa tagatatccc tgatattttt tcgcatattt gtgttgctct gtatctcaat
     3841 atatccttat cagatgtgtt tttcatgaga aaaagaaatt catctccgcc gatacgtccg
     3901 actatatcgc tctttctgga tacgtcccgt agtatctctc ctacgctgct caatatatga
     3961 tctcctgtct gatgccccat aaaatcgttg acctttttga agtcatcaat atctatgacc
     4021 ataagactgc acttttcctc acggctgtaa taatcaagat aaaaacggca gttctgagta
     4081 gttgtagcct tattgaaaat gcctgtcagg gggtccattg cgttcattcg ctgaagctga
     4141 agttttgttt tgttggctct aagtctgagg tcaagcacaa caaatgccat caatataccg
     4201 gccccacatg caattgaaga ctgaagcata tcaaaggcaa acgtgccgct gtccttgaac
     4261 atgaatacaa atgaacagaa gatgccctgg aaagccagta aaagaaaaat gtatattttc
     4321 atgggcatta tgaaaaccag aggaaggaat gtatatccca aaaaaagaaa tattgcggga
     4381 agatccggga aggggaacac acttatgaat atgaaaaaac tcaggaccat aacagaaaaa
     4441 acaaagcaaa gagcctggac cataaaaaaa cttccttctc ttttctggaa gtataacagg
     4501 caaaagatac aaaatactcc gaaaaccacc acaaaaaaat tgtatgctat gacaagctcc
     4561 acagaagtaa aatccttcaa gactacataa agatacatca tcaggcccat caatgctgaa
     4621 atactgtttc ttctaagtaa aaaaatgttg tcgttcttta tgtctttttt gcaattggaa
     4681 aaatatatct tactgtctct tatgaatccc ttaagatatc tcaccctttt atccctccca
     4741 tgccttggca acagcttcag ccactttttc tgccacaccg ggcataaatg cacttggtat
     4801 tatattttct tccgaaatat cttcttcgtt tatcatacct gccagtgcat atgctgcggc
     4861 ttttttcatt ttctctgtta ttctctttgc ccttactgac aaagccccct tgaatattcc
     4921 ggggaatatc agaacattgt ttatctgatt tgggaaatcc gatctgccgg tggctataac
     4981 ggcagcacct gctttttttg cttcttcggg cattatctca ggttccgggt tggccattgc
     5041 aaaaatcatg ggtgatcccg ccatggtttt taccattttc tgtgtaagtg cccctcctga
     5101 agagaccccg ataaaaagat ctctgcccgc caaagcctca gcaagttctc cggacaggtc
     5161 ctttttattt gttatactcg caagtttctt ttttgtttcg ttaagatcat ttcttttttt
     5221 atcgataatg ccatggctgt cacatacaac tatatcctca atacccatat ccataagcat
     5281 ttttgctatg gctgttcctg cggctcccgc tccgtttatg actgctttta tttcccaaag
     5341 ctttctgccg gtaagctttg ccgcatttat aagggcagcg gaaaccacaa ctgcagtacc
     5401 gtgctgatca tcatgaaaaa ccggtatctc cagttcaccc tggagttttt cttctatttc
     5461 aaaacatctg ggtgcggata tgtcttcaag attgatccct ccaaaagtgg aggaaatatt
     5521 ttttactatt tttactatct catcaggatc ctgggtcttt atacaaaccg gaaaagcatc
     5581 tatccctgca aattctctga acaaaagagc ctttccgtcc attacgggaa cagatgcagc
     5641 agcgccgata tttccaagtc ccagaacagc agacccgtcg cttacaaccg ccactgtgtt
     5701 gcttttgttt gtatatctgt aaatatcttc agggcattct ttgattttcc tgcagggttc
     5761 tgccaccccg ggagtatatg ctgttgagag gtcttctttt gtcttgaccc tcactttgcc
     5821 tgccgtggcc attttccctt tgtttttttc gtgcatgata agagaagcct tgtagtagtc
     5881 catttttttc tcctttattt tattttgaat gaactcttca gatccacaat gcggttgaat
     5941 actttttgtt tttcagaagt aaggattttg tcagctatat aatatccgtg tctgaaaaac
     6001 tggtatcttt cgcaaggcat tgcatttgcc acagaggcct ctgcataagc agttgcttct
     6061 ctgagagaat ccgcattgat ttttttgctt ccgtcatctc cctctgtcat tagatggtca
     6121 tagttccgta ttttgatctt caatgcattt tttgcatcca cccagtgtat cgtccctttg
     6181 actttccttc cgtcgaaacc gctgccgctc ctggtggcag ggtcatatgt acaatgcagt
     6241 tcttttattg tgccgtcagg attttttatc acatcattgc atgtgataaa ataggctcct
     6301 ttgaagcgca cttcattacc gggaaaaagt ctgaaatatt tttttaccgg cacttctgca
     6361 aaatcttcgc catccacata aagctccctg ctgaaggtca cttttcttgt tcccatttca
     6421 ggcacatttg cattgttttc tatttccatt tcttccgttt tatcttcggg ataatttgtg
     6481 attatcactt ttaacgggtt ctctaccaca ttcctggttt ctgtggtttt cttcagatcc
     6541 tctctcacgc aatgttccag gagggctata tcaacagtac tgttggactt ggcgacccct
     6601 atcctttcac agaaatccct tatagctgct ggagtatatc ccctgcgcct taatcctgct
     6661 atcgtaggca ttctcggatc gtcccagcct tcaacatctc cgttttccac aagggatttc
     6721 aaaagacgct tgctcattac ggttttggtt atattcagtc gggcaaattc tatctgtcgg
     6781 ggcggtgtct gccaccagcc gacttcggcg aggaaccagt cataaagcgg cctgtgatct
     6841 tcaaattcca gcgtgcatat tgaatgtgtt attccttcta tggcatcttc aattggatgt
     6901 gcaaaatcat acatgggata tatacaccac ttgtttttgg tattatgatg gggcacatgg
     6961 gctatcctgt aaatcacagg atctcgcata tttatattag gcgatgccat atcaattttt
     7021 gcccttaata ccttttctcc gtccgaatat tttccggctt tcatttcttc aaaaagttta
     7081 aggttttctt ctttgcttct tttacgatag gggctctctt taccggggtc ttttaatgta
     7141 cccctgtact cacgtatctc ttccgcatca agatcacaga caaaagcctt ttctttcttt
     7201 ataagttcta aagcggcttc atacattttt tcaaagtaat cggaagccca gagttttttg
     7261 tcccattgga aaccaagcca ctttacatcc tcttctatag aatcgatata ttcttcgtct
     7321 tcctttacgg gatttgtgtc atcatatctc agattgcatt taccctcgta tttcccggca
     7381 gtggaaaaat tcagacatat ggacttggca tgtcctatgt gaaggtatcc gttgggttcg
     7441 ggagggaatc ttgtatatac tctgcctccg tatttgcccg tttcattatc agcatcgata
     7501 atattatgaa taaaatttga agttatgttt tctttttctg acattgtttt cctccggtta
     7561 ttatatgcct gattattata tcacattttt ctcggctgtt cttcaataaa ttcgcagata
     7621 catgttataa ttgtcccata aaaaggaggg atataatgac tttaggatac aacaaggacg
     7681 gatttctgaa actgcccact ccattggaac ggcttgaagg catctcccgg gataccggtg
     7741 ttgatttctg gatcaaaaga gacgacctta cggatcccgg catgggagga aacaaactgc
     7801 gtaaacttga atacttcata catgatgccc tggataaaaa atctaccgtg ctgattacaa
     7861 caggagctcc tcaaacaaat cacggaagac ttactgccgc cgttgctgca aaatacggta
     7921 tgaaatgtgc catagtatca atagccgatc attccggcga gatttccggc aacatccttc
     7981 ttgaccgtat aatgggtgct gaagttttta taaaaaggcc ggagccgggg atttcagacg
     8041 gatcgctttt gtcagaaaca ttggcaaaaa ttacccggaa ctacgaaaat cagggagaaa
     8101 aagtttactt tgtaccaatg ggaggatcca gtg
//
LOCUS       DAONHK010000041         8098 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_6847_length_8098_cov_4.415517, whole genome shotgun sequence.
ACCESSION   DAONHK010000041 DAONHK010000000
VERSION     DAONHK010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 8098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8098
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6847_length_8098_cov_4.415517"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2675
                     /gene="ileS"
                     /locus_tag="PLM92_02600"
     CDS             <1..2675
                     /gene="ileS"
                     /locus_tag="PLM92_02600"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HPR24061.1"
                     /translation="LENDYIETVWWIQKEFFKAGLIYEGHKILPYCPRCGTGLASHEV
                     AQGYKEEQVLTVTTKFKLKEADNEYFLAWTTTPWTVPSNVALTVGPDLDYVKARMTEG
                     NEKGNIFYVAKVLAEKVLSVSGTYEILEELKGKDLEYKEYEQLMPYIKPEKKAFIVCC
                     GDYVTVEDGTGIVHTAPAFGEDDYSTGRKYDLPVINPVDDQGLFTATPWKGRFVMDEG
                     LDVEILKDLAKNNKVFSKEKMIHNYPHCWRCSTSLIYYAKPSWYIEMSKLREQLVSNN
                     NEVNWYPDFVGEKRFGNWLEDVKDWAISRNRYWGTPIPIWRCGCGHLECIGSRAELIE
                     RSIEDIDENIELHRPYVDDVHIECPVCGEKMERIPEVIDCWFDSGSMPFGQRHYPFEN
                     KENFDGDLFPADFICEGIDQTRGWFYSLMAISTFIKGTSPYKNCLVNDLILDKNGKKM
                     SKSRGNTVDPFTLFDKYGADATRWYMLHVSPAWAPTRFDEEGLKETVSKFFGTLKNVY
                     NFFVLYSNQDKVDPRDFDVPREKRPELDRWIISKYNKLIKEVEEDMDRYDHMKSVRKI
                     QYFVAEDLSNWYIRRARRRFWAEELTEDKKAVYATTYEVLKGVSQLIAPFAPFIADEI
                     YCNLTEDESVHISYFPEFDESLTDDFVEERMDLVRDIVGLGRGIREKERIKVRQPLRS
                     ILVDGKYASLIRDMVPLIKDELNVKDVIFEKDLTEYMSYSLKPDFKVAGPLLGGKIKA
                     FSAALSKEDPAEFVSRMEEEGKVFMDIEGENINITPEMTDIRIDAREGFAVAMEGNLF
                     AVLDTGLDSELLEEGLAREIVSKVQQLRKQKDFEMMDNIIITVDAGEEVKEAIKKHEK
                     YIMKETLATEIKEDKTKGKYNINGYDTGIDVEKI"
     gene            2688..2969
                     /locus_tag="PLM92_02605"
     CDS             2688..2969
                     /locus_tag="PLM92_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPR24062.1"
                     /translation="MAVTREELDRISKINEKEHAHIVDSFAVLGREPEKIAIRQGIDL
                     EDIILIIRAYDLQVGSEWGDERDDIGIWRKTPVAQIKDFVNDRYPGNLK"
     gene            2966..4012
                     /gene="rlmN"
                     /locus_tag="PLM92_02610"
     CDS             2966..4012
                     /gene="rlmN"
                     /locus_tag="PLM92_02610"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014062951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HPR24063.1"
                     /translation="MKDISGMTLPEMKTYFEKVGEKPYRATQVFRWIYRGVKNFDEMT
                     DLSKELRDDLKEKALISTPFIEEVKTSSSDETKKYLLSMKDGSLVESVLMKYDYGLSV
                     CISSQAGCAMGCSFCASAKGGKKRNLETGEMTGQILAIANDTGNKAGHVVVMGTGEPF
                     DNYENTSKFIKIINDKNGLNIGMRNITVSTCGIIPGIERFADEFPNVNLAVSLHSVSD
                     KTRTSLMPINRKYPLKELLKACKKYTDKTGRRITYEYALISGVNDGPRHIEALAEALK
                     GMLCHVNLIPLNEVCENPMTGPGRKRASKIAAELEKKGIPATVRRELGVDIDAACGQL
                     RSRKSREQRERSEQ"
     gene            4086..4508
                     /locus_tag="PLM92_02615"
     CDS             4086..4508
                     /locus_tag="PLM92_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008975105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPR24064.1"
                     /translation="MVKLLVGHKGSGKTKKMLHLANELVETCDGNVIFINKNEDLMYD
                     LKHDIRIVCMEDYPNITNSDEYIGFIYGIISSDHDIDTMFIDGILRHADVSLGNLPEF
                     IERIKKISNMFNIDFVVSLSAEKEEMIGVDFTDCEILN"
     gene            4572..5447
                     /locus_tag="PLM92_02620"
     CDS             4572..5447
                     /locus_tag="PLM92_02620"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPR24065.1"
                     /translation="MKKVYFFITLAAFLFGTMEVALKIAGSDMDPLQLTFLRFFIGGI
                     VLIPAAAGEMKSKKTKIRRQDLGRLLLLGIICIPVSMVLFQLGVDGSNASTAAVIFSV
                     NPLFTMILAHFFAGESMNRNKGIAFFLGLGGIIAMMRPWDVQSGNSISGMLFSVLAAA
                     FFGIYTVMGKNSVVRLGAIAQTCISFIMGSFVLLIIIIIMGRPVIAGISDDLLLLSYV
                     SIFITGLGYLAYFIAIRYSDATTGSVTFFIKPIIAPIIAALVLGDVILSSTYAGIGLI
                     LMASFININENRKSR"
     gene            5468..6088
                     /locus_tag="PLM92_02625"
     CDS             5468..6088
                     /locus_tag="PLM92_02625"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HPR24066.1"
                     /translation="MDINTIEEKIKDRRPGFVGQHTSFGVMVLLVDTKKGPSLLYEVR
                     AKTLSRQPGEVCFPGGQREKGETLEQCAVRETCEELGLEPAMIRVAGKLDTLHAHANF
                     SLYCYLGIVEQENIVASKINHDEVEEIFTVPLEVLLDTKPEIHYMQVRPYPEEDFPYD
                     KIDFAGGYKWRTGTLEVPIYTYEDKVIWGMTARITDNFMRIIKGED"
     gene            6088..6927
                     /locus_tag="PLM92_02630"
     CDS             6088..6927
                     /locus_tag="PLM92_02630"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HPR24067.1"
                     /translation="MKYKMALCQIKGSFQKEKSIGKAQAFIKEASEKAEVVALPEMWN
                     CPYGNKYFKEYAEEENGRTVLFMSKAAAQNDIYLIGGTIPEMEEGKIYNTSFCFDTRG
                     NLIAKHRKIHLFDVDIEDGIRFMESETLSAGDSVTVMETEYGGIGIAVCYDVRFPEWS
                     RAAALEGATLLIIPGAFNMTSGPAFWELMMRTRAIDNQVYVAAASPARNLSSSYHAWG
                     HSMIVNPWGGIMAEAGSGEEIIVATSYSYTELTVPEALAPPKTSTVFAGLKTALCSVI
                     NKG"
     gene            7701..7910
                     /locus_tag="PLM92_02635"
     CDS             7701..7910
                     /locus_tag="PLM92_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017729670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPR24068.1"
                     /translation="MKRLAVFVKERRRDVNLTQEEFAERAGVALTVIRKIEQGKTNLN
                     LDKVNLVLHMFGHELAPVNSKDLIE"
     gene            7907..>8098
                     /locus_tag="PLM92_02640"
     CDS             7907..>8098
                     /locus_tag="PLM92_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016991032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA N-terminal domain-containing protein"
                     /protein_id="HPR24069.1"
                     /translation="MRSGRVFYKDVLAGLITETNEGEFIFQYENHYVREHPNEFITFT
                     MPVSDQAYKSNRLFPFFEGL"
BASE COUNT         2696 a         1357 c         1920 g         2125 t
ORIGIN      
        1 ccctcgaaaa cgattatata gaaacagtat ggtggataca aaaggaattt ttcaaggcag
       61 gtctcatcta tgaagggcat aagatcctgc cttactgtcc ccgctgcggc accggattag
      121 catcccatga agtggcccag ggatacaaag aagagcaagt ccttacagtt accaccaaat
      181 tcaaactgaa ggaagcggac aatgaatatt ttcttgcatg gaccaccacc ccgtggacag
      241 tgccttcaaa tgtggctttg actgtgggtc ctgatcttga ttatgtaaaa gcaagaatga
      301 cagaaggaaa tgaaaaagga aatatattct atgtagccaa agttcttgct gaaaaagtcc
      361 tttcggtttc cggaacatat gagatcctgg aagaactcaa agggaaggat cttgaatata
      421 aagaatatga acagctaatg ccttatataa aacccgagaa aaaagccttt attgtatgct
      481 gcggagatta tgtaacagtt gaggacggta ccggtatagt acatacagcg cctgcttttg
      541 gagaagatga ctattcaaca ggcaggaagt atgaccttcc ggtgataaac cctgtggatg
      601 accagggact tttcacagcc actccgtgga agggtcgttt tgttatggat gaaggattgg
      661 atgttgagat actaaaagac cttgcaaaaa acaataaagt tttttcaaaa gaaaaaatga
      721 tacataatta tccccactgc tggagatgca gtacatcgct gatatattat gcaaaaccca
      781 gctggtacat agaaatgagc aagctgagag agcagctggt ttccaataat aatgaagtga
      841 actggtatcc ggattttgtg ggagaaaagc gttttggtaa ctggcttgaa gatgtaaagg
      901 actgggcaat atccaggaat cgttactggg gcacaccgat acccatatgg cgctgtggct
      961 gcggtcatct tgaatgtata ggaagccgtg cagaactgat tgaaagatcc attgaagata
     1021 tagatgaaaa catagaactg cacagacctt atgtggatga tgtccatata gaatgtcccg
     1081 tctgcggaga gaaaatggaa aggatccccg aagtgatcga ctgctggttc gacagcggat
     1141 caatgccctt cggacagaga cattatcctt ttgagaacaa agaaaatttt gatggggatc
     1201 tgttcccggc agatttcata tgtgaaggga tagatcagac aagaggatgg ttttattctc
     1261 ttatggccat atcgactttt ataaaaggaa catcacctta caagaactgt cttgtaaatg
     1321 atcttattct ggataagaac ggtaaaaaaa tgagtaagag tcgcggcaat actgtggatc
     1381 cgtttacact ttttgataaa tacggagcag atgccacaag atggtatatg ctgcatgttt
     1441 cccctgcatg ggctcccacc agatttgacg aagaaggact taaggagaca gtgagcaagt
     1501 ttttcggtac acttaaaaat gtatataact tttttgttct ttattccaat caggacaaag
     1561 tggatcccag agattttgat gtgcccaggg aaaaaagacc ggagcttgac agatggataa
     1621 tttcaaaata caacaagctc ataaaagaag tcgaagaaga catggacaga tatgatcaca
     1681 tgaaatctgt tcgtaagata cagtattttg ttgcggaaga tctttctaac tggtatataa
     1741 gaagggccag gagaaggttc tgggcagaag aacttaccga ggacaaaaaa gccgtatatg
     1801 caacaactta cgaagtatta aaaggtgtat cacagctgat cgcacccttt gcccccttta
     1861 tcgccgatga gatatactgt aatcttacag aagatgaatc agttcatatt tcatatttcc
     1921 ctgaatttga tgaaagcctt acggatgact ttgtagagga aagaatggat ttggtccggg
     1981 atattgtcgg acttggaaga ggaataaggg agaaggaaag gatcaaagtc agacagcctt
     2041 taagaagtat tcttgttgac ggtaaatatg cttcacttat aagggatatg gtacctctta
     2101 tcaaagatga attgaatgtc aaagacgtta tatttgaaaa ggatctgacg gaatatatga
     2161 gttacagttt gaaacctgat tttaaagttg cgggaccttt gctgggagga aagataaaag
     2221 ccttttcggc cgctttatca aaagaagatc cggcggagtt tgttagcaga atggaagaag
     2281 agggcaaagt tttcatggat attgagggag agaatatcaa tataactccg gagatgacag
     2341 atataagaat agatgccagg gaaggatttg ctgttgccat ggagggaaac ctctttgctg
     2401 ttctggatac aggacttgac agtgaacttt tggaagaagg gcttgccagg gaaatagtgt
     2461 cgaaagtcca gcagctcaga aagcagaaag attttgagat gatggataat ataatcatta
     2521 ccgttgatgc cggtgaagaa gtaaaggaag ccatcaaaaa gcatgagaag tacatcatga
     2581 aagagacact ggcaactgaa ataaaagaag ataaaacaaa gggaaaatat aatatcaacg
     2641 gatatgatac cggtattgat gttgaaaaaa tatgacagga gcagagaatg gcggtaacaa
     2701 gagaagaact tgacaggata tcaaagataa atgaaaaaga gcacgcacac atagtagaca
     2761 gtttcgccgt gcttgggagg gaaccggaga aaatcgcaat aagacagggg atcgatctgg
     2821 aagacataat actcataatc agagcttatg atcttcaggt gggaagtgaa tggggagatg
     2881 aaagggatga catcggtata tggcgcaaaa cacctgttgc tcaaataaag gattttgtta
     2941 acgatcgtta tccgggcaat ttgaaatgaa agacataagt ggcatgactt tgccggaaat
     3001 gaagacatac tttgaaaaag tcggagaaaa accatacaga gccactcagg ttttcaggtg
     3061 gatatatcgt ggtgtaaaaa actttgatga aatgacagat ctttcaaaag agctgagaga
     3121 tgatctgaaa gagaaagccc tgataagcac gcccttcata gaggaagtga aaacatcctc
     3181 atctgatgaa acaaaaaaat atcttctttc aatgaaagac ggaagtctcg ttgaaagtgt
     3241 gctcatgaaa tatgattacg gtttatccgt ttgcatatcc tcccaggcag gatgtgcaat
     3301 gggctgctca ttttgtgctt ctgccaaggg aggcaaaaaa agaaaccttg aaaccggaga
     3361 aatgacagga cagatactgg cgatagcaaa tgatacagga aacaaagccg gccatgtggt
     3421 tgtcatgggc acgggggaac cctttgataa ttatgagaac acatcaaaat tcataaaaat
     3481 cataaacgat aaaaatggtc tgaatatagg gatgagaaat atcacagttt ccacctgcgg
     3541 cattataccc gggatcgaaa gatttgcgga cgaatttcca aatgtcaatc tggccgtttc
     3601 tctccattct gtttcagata agaccaggac ctcactcatg cccataaaca ggaagtatcc
     3661 attgaaagaa ctgcttaaag cctgcaaaaa atacacagat aaaaccggaa gacggatcac
     3721 ttatgaatat gcgctgataa gcggagtcaa tgacggaccc cggcatattg aagctctggc
     3781 agaagcatta aaaggcatgc tttgtcatgt aaaccttata ccactgaatg aagtttgcga
     3841 aaatcccatg acaggtcccg gaaggaaaag agcctcaaag atcgcggcag agcttgaaaa
     3901 aaaaggcata cctgcgacag taagaagaga actgggcgtg gacatagatg cggcatgcgg
     3961 ccagctgaga tccagaaaaa gtcgggagca gcgggagcgg tcagaacagt aaaaattatc
     4021 tgttgcccgc ttatatttga tagtgtataa ttgatttata tttatatagc aaggaggcgt
     4081 tcgaaatggt gaaacttctg gttggtcaca agggtagcgg caagacaaaa aaaatgcttc
     4141 atctggccaa cgaacttgta gaaacatgtg atggaaatgt aattttcata aataaaaacg
     4201 aagatctcat gtatgatctg aaacatgaca ttcggatcgt atgcatggaa gattatccga
     4261 atataacaaa cagtgacgag tatataggct ttatttacgg aataataagt tccgatcatg
     4321 atatcgatac gatgttcatt gatgggatat taagacatgc tgatgtttct ctgggcaacc
     4381 tgcctgaatt cattgagaga ataaagaaaa tatcgaatat gttcaatata gattttgtcg
     4441 taagcctcag tgccgaaaaa gaagagatga taggcgtcga ttttacagat tgcgaaatac
     4501 tgaactgaaa gcaaaatgga taacaagagg gcggtgcatg gcactgctct cgttttttta
     4561 gaagaggcaa tatgaaaaaa gtatattttt ttataacatt ggcggctttc cttttcggca
     4621 ccatggaggt ggcactgaaa atagcaggaa gtgatatgga tcctctgcag cttactttcc
     4681 tgagattttt cataggagga atagtactga taccggcagc tgcaggtgaa atgaaaagca
     4741 aaaagacaaa aataagaaga caggacctgg gaagactgct tttactggga ataatatgta
     4801 ttcctgtaag catggtgttg tttcagctgg gtgtcgatgg ctcaaatgca tccacggcag
     4861 cagttatttt ttccgtcaat cccttgttca ccatgatact tgcacatttt tttgcgggag
     4921 agtcaatgaa tagaaataaa gggatagcct tttttctggg actgggcgga ataatcgcca
     4981 tgatgagacc gtgggatgtg cagtcgggaa acagcataag cgggatgctt ttttctgtac
     5041 ttgccgcagc ttttttcggc atatacacag tcatgggcaa aaatagcgtt gtaagactgg
     5101 gagctatcgc ccagacctgt ataagtttca ttatgggctc ttttgttttg ctgataataa
     5161 ttatcataat gggaaggcct gttatcgccg gcatatccga tgatctgctg ttattgtcat
     5221 atgtgagcat ttttataacg gggctggggt atctggcata ttttatagca atcagatatt
     5281 cggatgccac aacgggatct gtcacttttt tcataaaacc cataattgca ccaataatcg
     5341 cagccttggt tctgggagat gtaattttat caagcacata tgcggggata ggtcttatcc
     5401 tcatggcctc attcataaat ataaacgaaa acagaaaatc acgctgagcg gaaaggcaca
     5461 aagtatcatg gacataaata caattgaaga aaaaataaaa gacaggagac cgggatttgt
     5521 ggggcagcac acatcttttg gtgttatggt gcttcttgtg gacacaaaaa aagggccttc
     5581 tcttttgtat gaagtcagag cgaagacgtt atcaaggcag ccaggagagg tatgtttccc
     5641 cggaggtcaa agagaaaaag gagagaccct ggagcagtgt gctgtcaggg aaacatgcga
     5701 agaacttgga ctcgaacctg ctatgatcag ggtggcagga aaactggaca ctctccatgc
     5761 ccatgcgaat ttttctcttt actgctatct gggaatagtg gaacaagaaa acatagttgc
     5821 ttcaaaaatc aatcatgacg aagtggagga aatattcacc gttcctctgg aggttcttct
     5881 tgatacaaag cctgagatac actatatgca ggtgagaccg tatccggaag aagattttcc
     5941 ctatgacaag atagactttg ccggaggata caaatggaga acaggaaccc tggaagtgcc
     6001 tatttacact tatgaagata aagtgatatg gggaatgaca gccagaataa cagacaattt
     6061 catgagaata atcaaaggag aagattgatg aaatataaaa tggctctttg tcagataaaa
     6121 ggatcttttc aaaaagaaaa aagcattgga aaagcacagg ccttcataaa agaagcttct
     6181 gaaaaagcag aagtcgttgc attgcctgag atgtggaact gtccctacgg caataaatat
     6241 ttcaaagaat atgcggaaga agaaaacggc aggaccgttt tattcatgtc aaaggcggcg
     6301 gcacaaaacg atatttatct tatcgggggg accatacctg aaatggaaga aggaaaaatt
     6361 tataatactt cattctgctt tgacacacga ggtaacttga tagccaaaca caggaagata
     6421 catctctttg atgtggatat agaggacggc ataagattta tggaatcgga gaccctgtct
     6481 gcgggagatt ccgtaacggt catggaaaca gagtatggag gaataggcat agctgtctgc
     6541 tatgatgtcc gttttccgga atggagcaga gcagctgctt tggaaggggc aactctgtta
     6601 attattcccg gggcttttaa tatgacctct ggtccggcct tctgggaact gatgatgaga
     6661 acccgggcaa tagataatca agtctatgtg gcagcagctt ctccggcccg taacttgtca
     6721 tcgtcttacc atgcctgggg gcattccatg atcgttaatc cctggggagg gattatggca
     6781 gaagccggca gtggtgaaga gataattgtt gcaacatcgt attcatatac tgaattaact
     6841 gttcctgagg cattagcgcc tccaaaaacc agtacagtgt ttgctggact taaaactgcc
     6901 ctatgctcag ttattaataa aggataagga gctttggttt cccaggtatc agtggctgga
     6961 tcatacaatt caacaagatt agtataatcc tgaaatccaa tatcgcccgc aacctgtaat
     7021 acagtgttgt ttggcagtat tgttaaagtg tgtgtgttgc gtgcagctga aaagctactt
     7081 gtggattgcc agacagattg gctgaacatg gcaatcggta agaaaatgga aatgagtaaa
     7141 aagttaaatt taaacataat tttaatttgg tttgattaac aaaataaaca actgaaaatt
     7201 attaaatgaa aagtgagtgt tcaaatttta acaacttaca ttttccacgc atatattgaa
     7261 accagtgagg atttggtgcg tatagtcagc gaaagtcgga tagaaattcc cagcaatgta
     7321 aatgtttaat tttattacaa caatcagctt gagttatagg tcgttttgat caagaaaatg
     7381 gcgattgaat aagagaaaac tgtaattgat cagaacaatt gacaactgga gatgcctgac
     7441 gttgcttata attatcaaaa cggttaaatg acattggtaa tgtgctaagc gttaaaagta
     7501 atttttagct aaaaagtttt ggtggaatga gaattatgta taaggtttaa agtagttagt
     7561 tttggctggc attaattcat aaaaagcaaa actgaatcag cgtgtatgca aaagcatata
     7621 attgttaaaa cattcatgct atctatatta tattgcatat aattcataca tttgtgatgt
     7681 tattgatata attgtgtggc atgaaaagat tagcagtctt tgtaaaagaa cgtcggcgag
     7741 atgttaacct tacgcaggaa gaatttgctg agcgtgcggg tgttgcttta acagtgattc
     7801 gtaagataga acaaggcaaa accaatctga atttggataa agtaaattta gttttacaca
     7861 tgtttggtca cgaattggca cctgtaaatt ccaaagattt aatagaatga gaagcggtag
     7921 ggttttttat aaggatgttt tagccggatt aattacggaa acaaatgaag gtgaatttat
     7981 tttccaatat gaaaaccact atgtaaggga gcatcccaat gaattcatca cctttacgat
     8041 gcctgtttca gatcaggcct ataaaagtaa tcgacttttt ccgttttttg agggttta
//
LOCUS       DAONHK010000042         8025 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_6934_length_8025_cov_4.880678, whole genome shotgun sequence.
ACCESSION   DAONHK010000042 DAONHK010000000
VERSION     DAONHK010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 8025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8025
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6934_length_8025_cov_4.880678"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..781)
                     /gene="aguA"
                     /locus_tag="PLM92_02645"
     CDS             complement(<1..781)
                     /gene="aguA"
                     /locus_tag="PLM92_02645"
                     /EC_number="3.5.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047632 - agmatine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase"
                     /protein_id="HPR24070.1"
                     /translation="MAKRIDSTPKADGFRMPAEFEPQQQIFMIWPERPDNWRDGGKPA
                     QKAFADVAKAISEFAEMTMLVSASQYQVARNVLPPEIRVIEMSNNDSWVRDCGPTFVI
                     NDKGGIRGVDWKFNAWGGLFDGLYFPWDQDDLIARKICDMERIDTYRTEDFVLEGGSI
                     HVDGEGTVMTTEMCLLSEGRNPKMSREEIENMLKNHLNIEKVIWIKDVIDPEETNGHI
                     DDVACFVRPGEAACIWTDNKEDPFYETCKTAYETLTEATDAK"
     gene            complement(800..2128)
                     /locus_tag="PLM92_02650"
     CDS             complement(800..2128)
                     /locus_tag="PLM92_02650"
                     /inference="COORDINATES: protein motif:HMM:NF015515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HPR24071.1"
                     /translation="MELGFLTIVPLILMVVSVIVTKRVFESMIIPIILVFVLKDGTGF
                     IGGFVDSFYEVMAEGTFPWILMMLTLFGALIQLLLESGGIDGFRRLALRYIKAERSSL
                     VFTWILGVILCIDDYINDLAIGPSVRSITDSYKVPREAVGTVVISMGVPICSLLPVTA
                     MAVFVFGIMQDSGISGENSNMLTEYMKVVPFLVYPMLIVLVAFLLAVGILPKIGPLRK
                     LSKQMQEETEISEEHQHFDEKGQLLDFIIPVVVLVGVMLYTNDLVVAVLIAIAVCFAL
                     YIPRKKMKFDDFFKNFFEGVHSMIDILVILLMVFIFVDGLTRIGLSEYVIETTTPFLA
                     GGAIPVLTFIVVALLAFGGVDYWAVILLMAPITIPLAVSFDVNVYLTMAAAVSGAVCG
                     GTSCFFGEQMLMCSQSVEKKPTELAVANLPYSIIAFVGTAVIYAILGFVL"
     gene            complement(2130..3017)
                     /locus_tag="PLM92_02655"
     CDS             complement(2130..3017)
                     /locus_tag="PLM92_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HPR24072.1"
                     /translation="MKKGKVTIGMTQFACVADREENMQKAEEQIREMAAKGAKIICTQ
                     ELFNGTYFPQTIDYKKYNWAEPVDGPTNRRIQSLAEELDAVIISCYYEYAMDGIYYNS
                     AAVFDADGSFLGNYRKHHIPEGPQYIEKYYFTPGDSPYMVFHTKYGSFGVLICWDEWF
                     PEPSRILALKGADFIFYPSAIGSEPDDPKLDTSKTWMDSIRGHGIHNNFYVCACNRVG
                     EEKAEDGSGSMHFYGRSFVSDPWGNLKAEGKMNEEDLIVCELDLDEIRRARNILQFHR
                     DRRVDSYDEILNMSILERR"
     gene            complement(3281..4663)
                     /locus_tag="PLM92_02660"
     CDS             complement(3281..4663)
                     /locus_tag="PLM92_02660"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HPR24073.1"
                     /translation="MKKIPYEEIEKYLESIPGVVVIDMKGNVVYVNEQCAGYFGMSRD
                     SIYGKNIKEVFPQTKMIGGLKLDEPGLRFYNSYLGIGISVQVPIYEGNRKIGLLEYDV
                     TQSSEKLYDLSRDYGNFLDQELLNLEKEIIEKRDSRYSISSIIGGSEAIFEVKKKIIT
                     AAKSNSTVIIKGETGTGKELVAHAIHNLSGRRRNRIVKINATAFPETLIESELFGYEG
                     GAFTGAKRTGKKGKFEMADKGTLFIDEINNMPMSLQPKLLRVLQEREIERIGGNKDIS
                     VDVRVIAATNEDLQELIFENRFREDLYYRLNVIEIEVPALRARLEDIEDLVTEKIKEL
                     NIELGTDVERATAAAVNFLKKYNWPGNVRQLHNVVERAMNFSKGKELTKEDFEVAMAG
                     TCALGEKRKTKIGQSGRGSIEQARNNAEKELIEETLKMCGNNRTKAAECLDIARPLLY
                     QKMKRLGIKI"
     gene            complement(4788..6239)
                     /locus_tag="PLM92_02665"
     CDS             complement(4788..6239)
                     /locus_tag="PLM92_02665"
                     /inference="COORDINATES: protein motif:HMM:NF015047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HPR24074.1"
                     /translation="MDDAKKINEVMVSLFNIVLKIEEKAIKESTIHDLSITEMHTLDV
                     IGIGNGRTMSEVAQRLQINLSTLTIAINKLVKKGYVERFRIPSDRRIVKIKLTKEGFD
                     AVKEHEAFHDELIRKAIGKVSDEEIKNLIVSLDNIDTFMHMQMAKPIRDERDYALGKM
                     DFQNLHIKKALFQGGMGVGISLGGLAGAVAKEGGLGIISGSSTGFDEEDFEWDPQKAN
                     KRALKREIKKALEIADTEKSGGMIGVNIPYNTEDYREYVRTAVSEGAGAIITGAGLPM
                     TLPGICEREGYYDIALIPVISSLRAARVIIKNWQKKHKKRPDAFIFESAMAGGQLGFK
                     ESELLRARELYYKTITDIKSEIEDIPLIVAGGIFSRDDARNAYVYGADGIQLGTRFVT
                     TRECDAKESYKQAYINCREEDIVIIMNPQGAPCRALANKFTESLNGIDYDITKALVKA
                     AKGDVKNGLVFCGSKAYKTKKIDTVADIFKEFI"
     gene            complement(6256..6681)
                     /gene="fabZ"
                     /locus_tag="PLM92_02670"
     CDS             complement(6256..6681)
                     /gene="fabZ"
                     /locus_tag="PLM92_02670"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016207075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HPR24075.1"
                     /translation="MLFDKKQIMEIIPHREPFLLIDEVVEMETGRSITAIKHVRADEY
                     YFKGHFPDEPVMPGVLIVETLAQAGAVAVLSMEENRGRTAYFGGIREAKFRRKVMPGD
                     DLILKVKLDKMRSRAGTGECEAYVADELVCRCSILFVLG"
     gene            complement(6728..7951)
                     /gene="fabF"
                     /locus_tag="PLM92_02675"
     CDS             complement(6728..7951)
                     /gene="fabF"
                     /locus_tag="PLM92_02675"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HPR24076.1"
                     /translation="MNRVVITGMGAVTPIGKTVEESWKALKKGKNGIDKITHFDISEQ
                     KCKMGAEVKDFDYPDKRAARRMDRSSQFAVTAAAEALLDSGIESGENVSAERFGIMAG
                     TGIGGIMTLEEQVTKATKKESVKGVSALLVPMVISNMIGGNLAMQFKAKGSCLGIVSA
                     CSAGTHSIGEAYRNIKHGYADVMLGGAAEAAFAPVCFAGFGNMTATSTRTDKDRCSTP
                     FDKERDGFVMGEGSAFLVLESLENAKTRGARIYGEITGYGSTCDAYHITSPDPEGAGA
                     SGAMRMALREAGIMPGDIDYINAHGTGTPLNDLYETRAVKAVFGEKTKIPISSVKGNI
                     GHLLGAAGSIEALFSMKAMLDDFIPPTINLKVPDEELDLDYVPNEGRNQKLDCVMTNS
                     LGFGGHNGSLVIRKY"
BASE COUNT         1873 a         2054 c         1409 g         2689 t
ORIGIN      
        1 cttttgcatc tgtcgcttct gttaaagttt cataggctgt tttgcaggtc tcataaaatg
       61 gatcttcttt gttgtccgtc catatgcatg ccgcctcacc gggccttaca aaacatgcca
      121 catcatctat gtgtccgttg gtctcttcgg gatctataac gtcttttatc catattacct
      181 tttctatgtt cagatgattt ttcagcatat tttctatctc ttctctgctc atcttagggt
      241 tcctgccctc gctcaaaaga cacatctcag ttgtcatcac agtcccttct ccatccacat
      301 gtatacttcc gccttctaaa acgaaatcct ccgttctata tgtgtcgatt cgctccatgt
      361 cacatatctt ccgtgctatg agatcgtcct ggtcccaagg gaaatacaga ccgtcgaaaa
      421 ggcctcccca tgcattgaat ttccagtcta ctcctcttat cccgcctttg tcatttatta
      481 caaaagtcgg tccgcagtcc cttacccatg aatcgttgtt ggacatttct attactctta
      541 tttcgggagg aagcacattt ctggccacct gatactggga tgcggatacc agcatggtca
      601 tttccgcaaa ctcgctgatg gctttggcaa catcggcaaa ggctttttgg gcaggctttc
      661 ctccatccct ccagttatca ggtctttcag gccatatcat gaatatctgc tgctggggct
      721 caaactcagc cggcattctg aagccgtcgg cttttggtgt tgaatcaatt ctttttgcca
      781 ttactatttc ctcctttatt tataagacaa atccaagtat agcatatatt acagcggttc
      841 ccacaaaggc tattatgcta tagggcaaat tagccaccgc cagttcagtg ggctttttct
      901 ccactgactg ggagcacatg agcatctgct ctccaaagaa acatgaagtc ccaccgcata
      961 cagctcccga tacggctgct gccatggtta gatatacatt cacatcaaag ctgactgcca
     1021 ggggaatggt tatcggagcc atgagaagta tgaccgccca gtaatccact cctccgaagg
     1081 ccaaaagggc cacaacaatg aatgtcagca ccggtatagc gccgccggcc aggaacggtg
     1141 ttgtggtttc tataacatat tcactgagac ctattcttgt aagaccgtca acaaatatga
     1201 ataccatcaa cagtatcacc agtatatcga tcatgctgtg tacgccctca aagaaattct
     1261 tgaagaaatc atcgaatttc attttctttc tcggtatata caaagcgaaa caaaccgcaa
     1321 tggcaataag gaccgcaact accaggtcat tcgtataaag cattaccccg accaatacca
     1381 caacaggaat tataaaatcc agaagctgtc ctttttcatc gaaatgctga tgttcctcgg
     1441 atatttctgt ctcttcctgc atctgtttcg acaatttcct taacggtcca atctttggca
     1501 gtatgcccac tgccagaaga aaggccacaa gtactatcag catcggatat acaagaaaag
     1561 gaactacttt catgtattct gtgagcatgt tggaattttc acctgatata cccgaatcct
     1621 gcattatgcc gaaaacaaaa actgccatgg ctgttacagg cagcagagaa catatgggaa
     1681 cgcccatgga aataaccacc gttcccacgg cttctcttgg cactttgtag ctgtctgtta
     1741 tggatcttac cgaaggtcct atggccaggt cgtttatata gtcatctata caaaggatca
     1801 cgcccagtat ccatgtgaaa acaagactgc ttctttcggc tttgatatat cttaaggcca
     1861 gtctcctgaa accgtcaata cctcccgatt ctaaaagcaa ttgaataagt gcaccaaata
     1921 atgtaagcat catcagtatc cacgggaatg ttccttctgc catcacctca taaaatgaat
     1981 caacaaatcc tccgatgaat cccgttccgt ctttcagaac gaacaccagt attatgggga
     2041 ttatcatcga ctcaaatact cttttggtaa ctataaccga aactaccata agtatcagag
     2101 gtacaattgt taaaaatcca agttccatac tatctccttt ctaatatact catattcaat
     2161 atctcatcat aagaatcaac tcttctgtcc cggtggaact gcagaatgtt tctggcacgc
     2221 cttatctcat ccagatcaag ttcgcagact atcaggtcct cttcattcat ctttccttcg
     2281 gctttcaagt tcccccaggg atcggaaaca aagcttctgc cgtaaaagtg catgcttccg
     2341 ctgccgtctt cagctttttc ttctcctact ctgttgcagg cacagacata gaaattgtta
     2401 tggataccgt ggcctcttat ggaatccatc caggtttttg aagtgtcaag tttgggatca
     2461 tccggttcgg atccaattgc agagggataa aaaataaaat ccgctccctt taacgcaagt
     2521 attcttgaag gttccggaaa ccattcatcc cagcatataa gcacgccgaa actgccgtat
     2581 ttggtgtgaa agaccatgta cggagaatct cccggtgtaa agtagtattt ctcgatatac
     2641 tgtgggcctt cgggaatatg gtgttttctg tagttcccca gaaaactgcc gtctgcatcg
     2701 aatactgctg cggaattata atatatgccg tccatggcat attcgtaata acagcttatt
     2761 attacagcat caagctcttc tgccaaagac tggatccttc tattcgtggg tccgtccacc
     2821 ggttccgccc agttatattt tttgtagtcg atcgtctgcg gaaaataggt cccgttgaac
     2881 aactcctggg tacagattat ttttgcaccc tttgcagcca tttctcttat ctgctcttcc
     2941 gccttctgca tgttttcctc tctgtcagcc acacatgcaa actgcgtcat acctattgtc
     3001 acttttcctt ttttcatttt ccctcccgtc tttttttgtt gtaaatcatt gttttataat
     3061 tacgctctac ctattgacta taataatgca atatcagtgc caagtgactt tgacttatat
     3121 ttcaacgttt tttattaaca aaaaaagaaa gctgtaagtt tatacttaca actttcgctc
     3181 tttttatatt tttttgctta atatcacgac atttctatac ggtgtatgtt tataatcaca
     3241 ctgtcattct gcacttacat ctcctcccct tatccggatg ttatattttt attcccagcc
     3301 tcttcatctt ctgataaaga agaggtcttg cgatatcgag gcattctgcg gctttggtcc
     3361 tattgttacc gcacattttc aaagtttcct ctatcaattc tttttcggca ttgtttcttg
     3421 cctgttctat ggatcctctg ccgctttgtc cgattttggt ttttcttttt tcccccaggg
     3481 cacacgtgcc tgccattgct acctcaaagt cctcctttgt gagttccttt ccttttgaga
     3541 aattcatcgc cctttcgact acgttatgaa gctgcctgac atttcccggc cagttatatt
     3601 ttttcaggaa atttacggca gcagccgtgg ctctctccac atctgtacca agttcaatgt
     3661 tgagttcctt tattttttca gtaacaagat cttctatatc ctccagcctt gctctcaggg
     3721 cggggacttc tatctctatg acattcagtc tgtaataaag atcttccctg aatctgtttt
     3781 caaatatcag ttcctgcaga tcttcgtttg ttgcggctat cacccttaca tcaactgaaa
     3841 tatctttgtt cccgcctatt ctttctattt cacgctcctg aagcactcta agcagcttcg
     3901 gttgcaggga catgggcata ttgtttatct catctataaa aagcgttccc ttgtctgcca
     3961 tttcgaactt gccttttttg ccggttcttt ttgctccggt aaatgctcct ccttcatatc
     4021 cgaataattc ggattcaata agagtttcag gaaaagctgt tgcatttatc tttacaatgc
     4081 ggttccttct tctgccgctc aaattgtgta tggcatgagc taccagctcc ttgccggttc
     4141 ctgtttcacc ttttattatg accgtggaat tgctttttgc cgctgttatg atttttttct
     4201 tcacctcaaa aattgcttcg gaacctccga tgatactgct tatggagtat cgcgaatccc
     4261 ttttttcgat tatctctttt tccagattca atagttcctg gtcaagaaag ttaccatagt
     4321 ccctggagag atcataaagt ttctccgaac tttgggtcac atcatactcc agaagtccta
     4381 tttttctgtt cccctcatag atcggaacct gcacggatat tcctatcccc agatatgaat
     4441 tgtaaaatct aaggcccggt tcatccagct ttagtcctcc gatcattttt gtctgaggga
     4501 aaacttcttt tatatttttt ccgtagatcg aatcccttga catgccaaaa tagccggcac
     4561 actgctcgtt tacataaacg acattccctt tcatatctat aacaacgacg ccgggaatac
     4621 tttccagata cttttcgatt tcttcatatg ggattttttt cataacaccg gtcttctgcc
     4681 atatctcttt caaactattc taacatatat cccgacacta aaaaaccccg cgatctttta
     4741 ttttgcggga ttttctggtt ttcggggaaa tctttttttc ttgatggtca gatgaattcc
     4801 ttgaatatat ctgccaccgt atctattttt tttgttttat atgccttgct tccacagaac
     4861 acaagaccgt ttttcacatc tccctttgcc gcttttacaa gagctttagt tatatcatag
     4921 tcgataccat taaggctttc tgtaaatttg tttgccagag ctctgcatgg agccccctgc
     4981 ggattcatga ttatgactat atcctcttct ctgcagttta tatatgcctg cttatagctt
     5041 tctttggcat cacattctct ggtggtaacg aatctggttc ccagctgtat gccatcggcc
     5101 ccgtaaacat atgcgtttct tgcatcatca cgggagaata tccccccggc cactataagc
     5161 ggtatgtctt ctatctcact ttttatatca gtgatcgttt tataataaag ttcccgggct
     5221 cttaagagtt ccgattcctt gaaacccagc tgacctccgg ccatagcaga ctcaaaaata
     5281 aaggcatcgg gtcttttttt atgttttttc tgccaatttt tgattattac tctcgctgct
     5341 cttaatgagg atatgaccgg tatcagggct atatcgtaat aaccttctct ttcacatatg
     5401 cccggcaacg tcatagggag cccggctccg gttatgatcg ctcctgcacc ttcggagacg
     5461 gccgttctta catattccct gtaatcctca gtattgtatg gaatatttac acctatcatt
     5521 cctccgcttt tttctgtatc tgcaatttcc agggcctttt ttatttccct cttcaatgct
     5581 ctcttgtttg ctttttgggg atcccactcg aaatcctctt catcaaagcc agtgcttgaa
     5641 cctgatatta tgcccaggcc gccttctttg gctaccgctc cggccaggcc gccaagggat
     5701 atccccactc ccattcctcc ctggaataag gctttcttta tatgcagatt ctgaaaatcc
     5761 attttgccca gagcataatc tctttcatcc cttatgggtt tagccatctg catatgcatg
     5821 aatgtatcga tgttgtccag agaaacgatc agatttttga tttcttcatc ggatactttg
     5881 ccaatggctt ttcttataag ttcatcatga aaagcttcat gctctttcac tgcatcaaag
     5941 ccttcttttg taagttttat cttaactatc cttctgtcgc tgggtatacg gaatctttcg
     6001 acgtatccct tttttaccag tttgtttatg gctattgtca gagtggaaag atttatctgc
     6061 agcctttgag ccacttcgct cattgtacga ccgtttccta tgcctataac atccagggta
     6121 tgcatctctg taatggacag atcatgaatg gtggattctt ttatggcctt ttcttcgatc
     6181 tttagcacta tattaaagag gctcaccatt acttcattta ttttctttgc atcatccatt
     6241 actgctcctc ttttttcatc ccaaaacaaa caatatgctg catctgcaga ccagttcgtc
     6301 tgccacatag gcttcacatt cccctgttcc tgctctgctt ctcattttat ccaatttcac
     6361 cttcaatatc aagtcatctc ccggcattac tttccggcga aattttgctt ctcttattcc
     6421 accgaaatat gcagtcctgc cccggttctc ttccatggaa agtacagcca cagctccggc
     6481 ctgtgccaga gtttctacta tcaggactcc cggcattaca ggttcatccg ggaaatgtcc
     6541 cttgaaatag tattcatccg ctcttacatg ctttattgct gttatactcc tgcctgtttc
     6601 catttcaaca acttcgtcaa taagcagaaa aggttccctg tggggtatta tctccattat
     6661 ctgttttttg tcaaacaaca tgatcagatc cctctttctt ttctattttt cattttccct
     6721 tttacgccta atattttctt atcaccagag aaccgttatg cccgccgaag cccagggaat
     6781 tggtcattac acagtcaagc ttttgattcc tgccttcgtt gggcacataa tcaagatcaa
     6841 gttcttcatc gggaactttc agatttatag tgggaggtat gaagtcgtcc agcatggctt
     6901 tcattgaaaa aagcgcttca atacttccgg cagctccaag gagatgccct atgtttcctt
     6961 ttacggaact gatgggaatt tttgtttttt cgccaaaaac cgctttaact gcccttgtct
     7021 cgtaaaggtc attaagaggc gtgcctgtcc cgtgagcatt tatatagtct atgtcaccgg
     7081 gcatgatccc cgcctccctt agggccatcc tcatggcccc ggatgctcct gctccttccg
     7141 gatccggcga tgttatatga tatgcatcac atgtgctgcc atatccggtt atctctccgt
     7201 atatccttgc gcctcttgtc ttggcgtttt caagggattc aagaactagg aaagctgatc
     7261 cttctcccat cacgaatccg tccctctctt tatcaaaggg tgtagaacac ctgtccttgt
     7321 ctgtccttgt ggatgtggcc gtcatgttgc cgaatcccgc aaaacacaca ggcgcgaatg
     7381 cggcttctgc ggcacctccc agcatgacat cggcatatcc gtgttttatg tttctgtatg
     7441 cctctcctat gctgtgtgtc cctgctgaac aggctgatac tatgccaaga cagctgcctt
     7501 ttgccttgaa ctgcatcgcc agattccctc cgatcatatt tgagatcacc atgggtacca
     7561 gtaatgcgga tacgcctttt acactttctt tttttgttgc ttttgttacc tgttcctcca
     7621 gggtcattat cccgccgata cctgttcccg ccattatccc gaatctttcc gcagaaacat
     7681 tttctccgct ttctatacca ctgtcaagaa gggcttctgc cgcagcagtt acagcaaact
     7741 gtgaagatct gtccatcctt ctggcagccc ttttgtccgg ataatcaaaa tcctttactt
     7801 cggctcccat tttgcatttt tgttcactta tatcaaaatg tgttatttta tctataccgt
     7861 ttttgccctt tttaagggct ttccatgatt cttcaactgt ttttcctatg ggtgtcactg
     7921 cacccattcc ggttataact actctgttca tttatttctc ctcgtttttt atattataag
     7981 tccgccgtct acaccgatca cctgaccggt tatgtaatct cccat
//
LOCUS       DAONHK010000043         7959 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_7003_length_7959_cov_4.015309, whole genome shotgun sequence.
ACCESSION   DAONHK010000043 DAONHK010000000
VERSION     DAONHK010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 7959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7959
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7003_length_7959_cov_4.015309"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            26..1423
                     /locus_tag="PLM92_02680"
     CDS             26..1423
                     /locus_tag="PLM92_02680"
                     /EC_number="4.1.1.112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase subunit alpha"
                     /protein_id="HPR24077.1"
                     /translation="MAKVKITETVLRDGHQSLIATRMTTKEMLPILETMDEVGYHALE
                     MWGGATYDSCIRYLDEDPWDRLREIRKRIKKTNLQMLLRGQNLLGYKHYADDVVNEFV
                     DRAIANGIDIIRIFDALNDTRNLEAAVNATKRNGAHAQGCVSYTISPVHTNEMFVKLG
                     KEIEEMGCDSFCIKDMAGLLDPYNTYKLVKDLKKEISIPIELHTHATSGLGSLTYMKA
                     AEAGVDIIDTAISPFSGGTSQPPTEPLVAAFKGTKYDTGIEMEKLDLVSEHFRPFREK
                     YIESGLMDPKLMGVDINTLMYQVPGGMLSNLVSQLKEANSMEKFEAVLKEIPKVREDL
                     GYPPLVTPTSQIVGTQAVLNVVMGERYKMVPNEVRQLVKGMYGKTTVPIKKEIVDKIL
                     EGEEQITCRPADLIKPELEQAKKDCAVYMQQEEDVLTQAFFPKVAQNFFKKRMERMYK
                     LDLDLLNEEDSVYPV"
     gene            complement(1519..2679)
                     /locus_tag="PLM92_02685"
     CDS             complement(1519..2679)
                     /locus_tag="PLM92_02685"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPR24078.1"
                     /translation="MKLSRYTYLMMTGHICADITQGALPAILPFLIAEGNITYTSAAG
                     LVLATSSVSSLLQPVFGYMGDRGSNQWLMALGIFLAGTGFAIIGFMDSYWGMFTAAAV
                     SGAGVALFHPEGGKTANIVAGENKGMGMGLFGAGGNIGFTIGPMVASVTMVSFGIKGT
                     AFFFFPALIVTVIFLMSIKTLKIFMHMTASEKDKNGYYEDNENNWASFGRAVSIVFIR
                     SIINYGLLVFIPLYWVGILGQSEVSGSIKLTIMSGIGVFATLAGGKLADKLGFVPVIR
                     AGNILMIPFLILLLMTENILAATLLLIPIAFCNSVPYSAMIALSQSFLPRSVGLASGI
                     TLGLSVSIGGMVSPFMGKIADIYGLDAAMYVLLGFALVAVAISFILPKEKGS"
     gene            complement(3233..3946)
                     /locus_tag="PLM92_02690"
     CDS             complement(3233..3946)
                     /locus_tag="PLM92_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPR24079.1"
                     /translation="MIRKIINIDEEKCNGCGLCAEACHEGAIGMVSGKAKLLRDDYCD
                     GLGDCLPVCPTGAISFTEREAAAFDEKAVMENKKAKETEADTLPCGCPGTQSKKIKRD
                     ETPCYSAEAPLAPAASQLSQWPVQIQLAPINAPYFKDAHLLVAADCTAYAYGNFHNEF
                     MNNRTTLIGCPKLDPVDYTEKLTEIIRSNDIKSVTVVRMEVPCCGGIENAVKTTLQNS
                     GKFIPWQITTIATDGRILD"
     gene            4049..4723
                     /locus_tag="PLM92_02695"
     CDS             4049..4723
                     /locus_tag="PLM92_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009005408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HPR24080.1"
                     /translation="MNYKFLIKTSLFWGVSEKEAEDMLKCLGAHTQKYSKDEVVFPIG
                     KEIFEMGLVIEGSVNIIKEDVWGNRNIIDNIEVGQVFGEAYSFIRGEQLMGNVVAAEN
                     CEILFINADRITKTCSSGCEFHSRLVQNLLSVISLQNLKLTRKINHITQKSTKDKVLS
                     YLSYQAMKQGKYAFDVPFNRQEMADYLSVERSALSNELSGMQKEGLISYRKNHFVLHC
                     TAEDYS"
     gene            complement(4741..5388)
                     /locus_tag="PLM92_02700"
     CDS             complement(4741..5388)
                     /locus_tag="PLM92_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPR24081.1"
                     /translation="MKTVYLSEKADPRLKRYIRECGYDIFNVKATSFVSGPVSSHADI
                     YYCKLGCNDGAPVFCDKKEKLGYFYPSDIIYNAACTGKFFIHDLRHTDRTLMEKAKDM
                     EMIFIDVKQGYSKCSICVVDENSVITADQGIAKKISKEKGPDCLLISPGHIILERHEY
                     GFIGGSCGLIGNEIVFNGDISLHPDFKRISDHIQSRGLSLKWFPGRELEDIGSLL"
     gene            complement(5385..6338)
                     /locus_tag="PLM92_02705"
     CDS             complement(5385..6338)
                     /locus_tag="PLM92_02705"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009644595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HPR24082.1"
                     /translation="MEKFNYTVTKEDHENKLRISELVRRRFGFSSRMRSRIKREGKVM
                     RNGEPVNIWEIPREKDLISIQLPEEVSNFEPEQIPIDVIYEDDDLFIINKQPGVVVHP
                     TKGHPAHTIANGMANYMNETGNHFKMRFINRLDMDTSGVLLLAKSAYCQNDFMKQMER
                     NRVEKKYTAIVLGLMENDSGEIDLPIGRPSPESLTRCVKEDGRPSVTRYNVIERFHLP
                     NSHMDSEDGIIRTKGYTLVDILLVTGRTHQIRVHLSHMGHPVAGDHLYGGEKIHLIER
                     QALHAKSISFLHPVTGKPVSVEAPMPSDMKDALNKLRKLRK"
     gene            complement(6343..7146)
                     /locus_tag="PLM92_02710"
     CDS             complement(6343..7146)
                     /locus_tag="PLM92_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006000554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HPR24083.1"
                     /translation="MKKRIINIFSNDLPASENICSLLKEKLERSGFVVPGVFSHDAEL
                     LVCVGGDGATLEAINKYDFPKVPIVGINTGHLGFFQEINPNQLDEFITNYKAGKYRLQ
                     PLSTVQATVLSEDGPRIHRGLNEIVIKGWKSYSAHLNISIGGSFIERFSGDGIIISTP
                     AGSTAYNYSLGGSIIDPRLKALQVAPIAPMNTTAYRSFTSSILLPSDLSLDVVPEYIK
                     SNQIYLINDGIETEYRNVTSIKVEFSDTVVNLLRFETYDFWNKVKTKFL"
     gene            complement(7156..>7959)
                     /locus_tag="PLM92_02715"
     CDS             complement(7156..>7959)
                     /locus_tag="PLM92_02715"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006000555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HPR24084.1"
                     /translation="SPLGDKYISAMKKGISRGWIDVYENRGKTSGAYSFGSYDSKPFI
                     LLNYTDTLKDVFTLAHEMGHSMHSFYTRKNQPFIYGGHSIFTAEVASTVNENLLMKYL
                     LSKEKDPEMKKYLLNMQIEGFRTTLFRQTMFAEFEQLTHEAVEAGETLTADWLCDEYD
                     KLNNKYFGPSLCHDDLIKYEWARIPHFYNAFYVYKYATGYSAASAISDMIIAGTKNRN
                     NYIEFLKTGEKDYPVELLKIAGVDMGSPAPVERAMSVFKKLVEEFETLV"
BASE COUNT         2321 a         1607 c         1791 g         2240 t
ORIGIN      
        1 aagtacaaaa gttacggagg gtaaaatggc aaaagtaaaa ataacggaaa cggttttgcg
       61 ggacgggcat cagtcactga tagcaacgag gatgacaaca aaagaaatgc tgcccatact
      121 tgagaccatg gatgaagtgg gctatcatgc tcttgagatg tggggcggag ccacttatga
      181 ttcatgcata agatatctgg atgaggatcc atgggacagg ctcagagaaa taagaaaaag
      241 aataaagaaa acaaatctgc agatgctttt gagaggtcag aaccttcttg gatacaagca
      301 ttatgcggat gatgtggtaa atgaatttgt agacagagcc atagcaaacg gaatagatat
      361 tataagaata tttgatgccc tgaatgatac gaggaatctg gaagcggcgg tcaatgccac
      421 aaagagaaac ggagcccatg cccagggatg tgtatcatat acgatcagcc cggtgcacac
      481 aaatgaaatg tttgtaaaac tgggcaaaga aatcgaagaa atgggatgcg actcattttg
      541 cataaaagac atggcaggcc tgcttgatcc ctataacacc tataaactgg taaaagacct
      601 taaaaaagaa ataagcatac ctatagaact tcacacccat gccaccagcg gactggggag
      661 tctgacatac atgaaagcgg cagaagcagg agtggatata atagacacag ctatctcacc
      721 tttctcagga gggacatctc agcctcccac agaacccctt gtggcggcat tcaaaggcac
      781 taaatacgat acaggcattg aaatggaaaa acttgatctg gtgtctgaac acttcaggcc
      841 tttcagagaa aagtatatag aaagcggtct gatggatcca aaactcatgg gtgttgatat
      901 aaatacactg atgtatcagg tgcccggagg gatgctttca aatctggttt cccagctgaa
      961 agaagcaaat tccatggaga aattcgaggc cgtgctgaaa gaaataccca aggtaaggga
     1021 agatttgggc tatccgccac tggtaacacc aacaagccag atagtgggta ctcaggcagt
     1081 tttgaatgtg gtcatgggag aacgttacaa gatggtacca aatgaagtaa gacagcttgt
     1141 aaagggcatg tacggaaaaa ccacagtacc aataaaaaaa gagatagtgg ataagatcct
     1201 tgaaggagaa gaacagataa cctgcagacc tgcagatctt atcaaaccgg aacttgaaca
     1261 ggccaaaaag gattgcgcag tatatatgca gcaggaagaa gatgtgctga cacaggcgtt
     1321 ttttcccaaa gtggctcaga atttcttcaa aaaaaggatg gaaagaatgt acaaacttga
     1381 tttggatctt ctgaatgaag aagactcggt ctatccggta taagcatttt taatagtgaa
     1441 aaaaaagaag accgggtgtc gcaagaagcc tccccggttt ttttgtgttt tattatgaat
     1501 atgattccgt aacaaaaatc aggaaccctt ttcttttgga agaataaaag aaatagcgac
     1561 tgcgaccaaa gcaaatccca gtagcacata cattgctgcg tcaagcccgt aaatatcagc
     1621 tatttttccc atgaaaggag agaccatacc tcctatactg acagaaagtc cgagggttat
     1681 tcccgaggcc agacctacag acctgggaag aaagctctgg cttagagcga tcatggcact
     1741 gtaggggacg ctgttgcaga aagctatggg tatgagcaac agggtcgcag ccaaaatatt
     1801 ttcggtcatg agcagcagta taagaaaagg tatcatgagt atattacctg cccgtattac
     1861 aggtacgaat cccagtttgt cggcaagttt gcctcccgcc agggtggcaa aaacacctat
     1921 cccggacata atagtaagct taatgcttcc tgaaacctcc gactgaccaa ggataccgac
     1981 ccagtaaaga ggaatgaaaa ccagaagacc gtaattgatt atggatctga taaatactat
     2041 ggatacagcc ctgccaaagg aagcccagtt attttcgtta tcttcatagt atccgttttt
     2101 atccttttca gatgcagtca tgtgcataaa tattttgagc gtttttatgg acatcaaaaa
     2161 aatgacagtg acaatcagtg caggaaagaa aaagaaggcg gtgcctttga taccaaagct
     2221 gaccatagta acggaggcca ccatgggacc aatggtaaac ccaatgttgc ctccggctcc
     2281 gaaaagaccc attcccattc ctttgttttc accggcaacg atgttggctg tttttcctcc
     2341 ttctggatgg aaaagggcaa caccggcgcc cgatactgcg gcggcagtaa acatgcccca
     2401 gtaggaatcc atgaaaccta tgatggcaaa accggttccc gcaagaaata tgcccagagc
     2461 cataagccac tggttggaac cccggtctcc catataaccg aaaacgggct gcagcagaga
     2521 tgataccgag ctggtggcca gaacaagtcc ggcggcggat gtatatgtta tattgccttc
     2581 tgcaatcaga aaaggaagaa tggcaggaag cgctccctgc gttatgtctg cgcatatatg
     2641 acctgtcatc ataagataag tgtatctgct tagcttcatt tatactttac ctttcctttg
     2701 catctgaaag ggagtatatc agaaatctta cgtatttcca ataataaaca tagatgcaaa
     2761 ataaaagacg acgaagaaaa atgacatgtt taagcttttt gataaggggt ataacaagtc
     2821 cataagaaaa attcactgga ggtggtattt atgaaaatcg tgttgaacga agatatcaaa
     2881 gcctatatga acgaaaataa catttctgac gtagtggtgg atgttataag gagaagttgc
     2941 tgaagcggtt cctatgagga tatatctgca agttttgcag atgaggaaga cagagaaaga
     3001 tacgtagaag caggcttttt ggaagaggat tcggaactgg gcaaggtatt tttttcgcct
     3061 tcgataagat ttgagggaga agaggtagga ttgaacctta aaaagtttct ctggtcaaag
     3121 agtattgaca tatcaggcat aagaagataa aagagactgc tgtagattta cagcagtctt
     3181 ttcttttttg catatatata ttttgtggtt tataattctt ccggccggga tctcaatcaa
     3241 gtatcctgcc gtcagtggct attgtcgtta tctgccaggg aatgaatttt ccgctgtttt
     3301 gaagggttgt ttttacggca ttttctattc ctccgcagca tggcacctcc attcttacga
     3361 ctgtaacgct ttttatatcg ttgcttctta ttatttctgt aagtttttca gtgtagtcaa
     3421 cgggatccag tttcgggcat cctatcagcg tggttctgtt attcatgaat tcattatgga
     3481 aattaccgta ggcatatgcc gtacagtcgg cagccaccag cagatgggca tccttgaaat
     3541 aaggcgcatt tattggggcc agctggatct gtaccggcca ctgtgaaagc tgtgaagctg
     3601 ccggagccaa aggagcttca gctgaatagc agggagtttc atctcttttt attttttttg
     3661 actgggtgcc ggggcatccg cagggcagag tatctgcctc agtttctttt gcttttttat
     3721 tttccatgac ggccttttcg tcaaatgcgg cagcctccct ttcggtgaag gatatggcac
     3781 ctgtgggaca tacaggaaga cagtccccaa gaccgtcaca gtaatcgtcc cgcagaagtt
     3841 ttgcttttcc gcttaccatt ccaatagcac cttcatggca tgcttcggca caaagaccgc
     3901 agccgttgca tttttcttca tcgatattta ttatttttct tatcatctca acacctctta
     3961 tatatttttc acttgtatta atacctgcac aatactataa taaaaacaga aagtatgttg
     4021 taaaaagaac gaaaaggaga aaaaagtgat aaattataaa ttccttataa aaacatcgct
     4081 tttttggggc gtttcagaaa aagaagcgga agatatgctg aaatgcctcg gagcgcatac
     4141 gcagaaatat tcaaaagatg aagtcgtttt tcccataggc aaggaaatat ttgaaatggg
     4201 acttgttatt gaaggttcgg taaatattat caaagaagat gtctggggaa acaggaatat
     4261 aatagacaat attgaagttg gacaggtttt cggtgaagca tactcattta tccggggaga
     4321 acagctcatg ggcaatgtag tcgctgctga aaactgtgaa atacttttta taaatgccga
     4381 tagaataaca aagacatgtt cttcaggatg tgagtttcac agcagactgg tgcagaacct
     4441 gctgtcggtg atatctctgc agaatctcaa gcttaccaga aaaataaatc atataaccca
     4501 aaaatcaaca aaagacaagg ttttatccta cctttcatat caggccatga agcaggggaa
     4561 atatgccttt gatgtgccct ttaacagaca ggaaatggcc gattatcttt cggtggaaag
     4621 aagtgctctt tccaatgagc tttccggtat gcagaaggaa ggtcttatat catacagaaa
     4681 aaatcatttt gttctgcatt gcactgcaga ggattattca taaaagccaa aaactcaaga
     4741 tcaaagcaaa gaaccgatat cttcaagttc tcttccggga aaccacttaa gggaaaggcc
     4801 ccgggattgg atgtgatccg atatcctttt gaaatccggg tgaagagaaa tgtccccgtt
     4861 aaaaacgatc tcgtttccga tgaggccgca ggaacctcct ataaagccgt actcatgtct
     4921 ttctaatata atatgtcccg ggcttatcag cagacagtcc gggccttttt ctttggatat
     4981 tttttttgct atgccttgat ctgccgttat tacagagttt tcatcaacga cacatatgct
     5041 gcatttggaa tacccctgtt taacatcgat aaaaatcatt tccatgtctt tggctttttc
     5101 cattagggta cggtcggtat gcctgagatc gtgaataaag aattttcccg tacaggcggc
     5161 attatatatt atatccgaag gataaaagta gccgagtttt tctttcttgt cgcagaatac
     5221 gggggcaccg tcattgcagc ccagcttaca gtaataaata tcggcatggg atgaaacggg
     5281 cccggaaacg aagcttgttg cttttacatt gaaaatatcg tatccgcatt ctctgatata
     5341 tcttttcagg cgggggtctg ccttttctga caaatacacg gttttcattt tcttagtttc
     5401 ctcagtttgt tcagggcatc tttcatatcg gagggcatag gagcttcgac agagacaggt
     5461 tttccggtga cgggatgaag gaaggaaata ctttttgcat gcagagcctg tctttctata
     5521 agatgtattt tttctccgcc gtataggtga tcgcctgcca caggatggcc catgtgggac
     5581 aagtgtactc tgatctggtg tgtccttccg gtaacaagaa gtatatcaac aagggtatac
     5641 ccctttgttc tgattatacc gtcttcgctg tccatatggc tattcggcag atgaaatctt
     5701 tctatgacat tatagcgggt aacggaaggg cgtccgtctt cttttacgca tctggtcaga
     5761 ctttcgggag aaggtcttcc tatgggaaga tcgatttctc cgctgtcgtt ttccatcaaa
     5821 cccaaaacta tggcagtata ttttttttcc acacggtttc gttccatctg cttcatgaaa
     5881 tcattttgac aataggcgct tttggctaaa agcagtacac ctgaggtgtc catgtccagc
     5941 ctgtttataa agcgcatttt gaaatggttt cccgtctcat tcatgtagtt tgccataccg
     6001 ttggcaattg tatgggcagg atgacctttt gtgggatgta ccaccactcc cggctgcttg
     6061 tttataataa aaagatcatc atcctcatat atgacatcaa tgggtatttg ctccggttca
     6121 aaattgctga cttcttcagg aagctgtata cttattaaat ctttttcccg gggtatttcc
     6181 cagatattca ccggctcccc gtttcgcatc acttttcctt ctctcttgat cctgcttcgc
     6241 atacgggaag agaaaccgaa tcttcgacga acaagctccg atatacggag cttgttttca
     6301 tgatcctctt ttgtaactgt gtagttgaat ttttccattt tattacaaaa actttgtttt
     6361 tactttgttc cagaaatcat aagtttcaaa acggagaaga ttgaccaccg tatcggaaaa
     6421 ctccactttt atgctggtca cgttgcggta ttcggtttcg atcccgtcat ttatcagata
     6481 tatctggttg ctttttatat attcgggaac tacatccaga gaaagatccg aaggcagcag
     6541 tatgcttgag gtgaaagagc ggtatgcagt ggtattcata ggtgctatag gagccacttg
     6601 cagcgctttg agacgcgggt ctattattga gcctcccagt gagtaattgt atgcggtgct
     6661 tcctgcaggg gtggatatta ttattccatc tccgctgaac ctttctatga aactgccacc
     6721 tatggaaata ttcaggtgag cagagtatga cttccatcct ttgatgacta tttcattaag
     6781 ccctctgtgt atacgaggac cgtcttccga taaaactgtg gcctgtaccg tggaaagggg
     6841 ctgcagtctg tatttgccgg ccttgtaatt ggtaatgaac tcatccagct gattcggatt
     6901 tatctcctgg aaaaagccca gatgccccgt attgatgccg actatgggga ctttgggaaa
     6961 gtcatatttg ttgatggctt caagagtcgc accgtcaccg cctacgcaga ccagaagttc
     7021 tgcatcatgt gaaaaaacac cgggaaccac aaaaccgctg cgttcaagtt tttctttcag
     7081 cagactgcag atattttctg atgccggtag atcatttgaa aaaatgttta ttattctttt
     7141 tttcatgatt atatgttaca ccaaggtttc gaattcttca acaagctttt tgaatactga
     7201 catggccctt tctacgggag caggacttcc catatccaca ccggcgatct taagaagttc
     7261 aacaggatag tctttttcac cggtcttaag gaactctata tagttattgc ggttttttgt
     7321 gcctgcgatt atcatgtctg aaatagccga ggccgctgaa taaccggtgg catatttata
     7381 aacataaaaa gcattataga agtgaggtat cctggcccac tcatatttta tcagatcgtc
     7441 atggcaaaga gacggaccga aatatttgtt attgagcttg tcatattcat cgcaaagcca
     7501 gtcggcagta agggtttctc ccgcctctac cgcctcatgg gtaagctgtt cgaattcggc
     7561 aaacattgtc tgccggaaaa gggtggtgcg aaatccctcg atttgcatat tgaggagata
     7621 ttttttcatt tcagggtctt tttcttttga aagaagatat ttcataagca gattctcatt
     7681 gacagttgat gccacctctg cagtgaatat ggaatgacct ccatatatga aaggctggtt
     7741 ttttcttgta taaaatgaat gcatcgaatg gcccatttcg tgggcaagag tgaaaacatc
     7801 ctttagtgta tctgtgtaat tgagaaggat gaaaggcttg ctgtcatagc tgccaaaact
     7861 gtatgcgcca ctggttttgc cgcggttttc gtatacatct atccagcccc gggaaatacc
     7921 ctttttcatg gcggaaatat acttgtctcc caaaggaga
//
LOCUS       DAONHK010000044         7853 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_7133_length_7853_cov_4.205309, whole genome shotgun sequence.
ACCESSION   DAONHK010000044 DAONHK010000000
VERSION     DAONHK010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 7853)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7853)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7853
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7133_length_7853_cov_4.205309"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1195)
                     /gene="secY"
                     /locus_tag="PLM92_02720"
     CDS             complement(<1..1195)
                     /gene="secY"
                     /locus_tag="PLM92_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005838613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HPR24085.1"
                     /translation="MLKTIAKAWKIEDIRRKIIFTLLMLVVFRIGSNIPVPGIDREIL
                     NQAFQGGTGLLELFDLFSGGSFSNMTIFALSITPYITASIILQLLTIAIPALERLSRE
                     GTEGRKKITQYTRYLTVALAFVQAIGVSVGMFRQALISTDFFSVAVIVIVLSAGTAFL
                     MWLGEKINENGIGNGISLIIFAGIVARLPSAIRETLTKYQAGEIGFLTIALFVIFAII
                     VIVGIIEVQHGQRRIPVQYAKRVVGRKMYGGQSTHIPLKVNQAGVIPVIFALSMLQFP
                     LTVTYFAGTDTDFTQWIEKWLSPVDSPGIWVYAVFNVVLIIFFTYFYTAVTFNPMEVA
                     NNMKANGGFIPGIRPGRTTVEYLNKVMTRITFVGAVFLAAVATLPTIVGQITPLDVQF
                     GGTSLL"
     gene            complement(1203..1643)
                     /gene="rplO"
                     /locus_tag="PLM92_02725"
     CDS             complement(1203..1643)
                     /gene="rplO"
                     /locus_tag="PLM92_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003357513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HPR24086.1"
                     /translation="MKLHELKAAPGSAKTGKRKGRGHATGQGTTAGRGMNGQNSRSGG
                     GVRLGFEGGQMPLYRRLPKRGFTNIWGTKYAELNVDALNRFDAGTVVTPEMLAESGLV
                     KQVKDGIKILGNGKLEKNITVRAHKFSKTAVEKIESAGGKAEVI"
     gene            complement(1663..1845)
                     /gene="rpmD"
                     /locus_tag="PLM92_02730"
     CDS             complement(1663..1845)
                     /gene="rpmD"
                     /locus_tag="PLM92_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HPR24087.1"
                     /translation="MAKMIKITLTKSTIGASPKQRKVVEALGLKKMHQSVELVDSPVT
                     RGAVEKVKHLVTLEEL"
     gene            complement(1858..2361)
                     /gene="rpsE"
                     /locus_tag="PLM92_02735"
     CDS             complement(1858..2361)
                     /gene="rpsE"
                     /locus_tag="PLM92_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005999428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HPR24088.1"
                     /translation="MRNTIDVSRLDLKETIVNIRRVAKTVKGGRNMRFSVTVVVGDGE
                     GHVGVGLGKAQEIPEAVRKATEDAKKNVINVPTVGTTIPHRILGVFGAGRVLIMPAAE
                     GTGVIAGSSVRTVLESAGVKDVRAKSIGSNNTGNMAYATMEGLKNLMTVEKISKLRGK
                     TKEEIFG"
     gene            complement(2376..2741)
                     /gene="rplR"
                     /locus_tag="PLM92_02740"
     CDS             complement(2376..2741)
                     /gene="rplR"
                     /locus_tag="PLM92_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005999426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HPR24089.1"
                     /translation="MAKESRNERRIARHRRTRQNLHGTPEKPRLCVFRSNKNISVQII
                     DDVNGNTLVAASSLDKGLKNDIKYGGNKEAAKKVGEAIAKRALEKGIETVVFDRGGSL
                     YHGRVKELADGAREAGLKF"
     gene            complement(2758..3309)
                     /gene="rplF"
                     /locus_tag="PLM92_02745"
     CDS             complement(2758..3309)
                     /gene="rplF"
                     /locus_tag="PLM92_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009644414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HPR24090.1"
                     /translation="MSRIGIRPIDLPAGVEVKVDEKNVVTVKGPKGELQEKISKLIKI
                     EQKDGVLNIKRDSDEKENRSQHGLARSLISNMVEGVTKGFEKKLELQGVGYKAEKKGN
                     KLVLNVGYSHPVELEDPKEITTEVPEATKIIVKGISKQAVGNYAADIRAWRRPEPYKG
                     KGIRYEGEYVRRKEGKAGVKASE"
     gene            complement(3322..3720)
                     /gene="rpsH"
                     /locus_tag="PLM92_02750"
     CDS             complement(3322..3720)
                     /gene="rpsH"
                     /locus_tag="PLM92_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HPR24091.1"
                     /translation="MTMTDPIADMLTRIRNANTVGHETVDIPASKIKKSIAGILAEEG
                     YIKGFDVIEDDKQGTIRVQMKYGPDKDKVISGIKKISKPGLKVYAKASDVPRVLGGLG
                     IAIISTSNGVISDKEARKLGVGGEVICYVW"
     gene            complement(3799..3984)
                     /locus_tag="PLM92_02755"
     CDS             complement(3799..3984)
                     /locus_tag="PLM92_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008752089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HPR24092.1"
                     /translation="MAKTSLKIKQQRKPKYQTRAYTRCRICGRPHSVLKKYGICRICF
                     RELAYRGEIPGVKKASW"
     gene            complement(3994..4536)
                     /gene="rplE"
                     /locus_tag="PLM92_02760"
     CDS             complement(3994..4536)
                     /gene="rplE"
                     /locus_tag="PLM92_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HPR24093.1"
                     /translation="MTARLKEKYKKEAFPAMMEQFKYENVMEVPKLDKITINIGLGEA
                     KDNPKAMETATAELALIAGQRPVVTKAKKSIANFKVRQGMPVGTKVTLRGEGMYEFAD
                     KFFNIALPRVRDFKGVSKNAFDGRGNYSVGIKEQLIFPEINFDKVDMVKGMNIVFTTT
                     AKTDEEAAALLGFLGMPFEK"
     gene            complement(4554..4859)
                     /gene="rplX"
                     /locus_tag="PLM92_02765"
     CDS             complement(4554..4859)
                     /gene="rplX"
                     /locus_tag="PLM92_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HPR24094.1"
                     /translation="MRIKKDDMVIVLTGKDKGKKGKVLKAMPKKNKVLVEGINMQTKH
                     QKQTRTAAAEIKHQEGPLDISNVMLIDSKTKEPTRVGYKMEGDKKVRIAKKSGTVIE"
     gene            complement(4869..5237)
                     /gene="rplN"
                     /locus_tag="PLM92_02770"
     CDS             complement(4869..5237)
                     /gene="rplN"
                     /locus_tag="PLM92_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HPR24095.1"
                     /translation="MIQVESRLRVADNSGAKELLCIRILGGTRRQYANIGDIIVCAVK
                     EATPGGLVKKGEVVRAVVVRTKHGARRVDGSYVKFDQNAAVIIKEDKNPMGTRIFGPV
                     ARELRDKSFMKIVSLAPEVL"
     gene            complement(5252..5512)
                     /gene="rpsQ"
                     /locus_tag="PLM92_02775"
     CDS             complement(5252..5512)
                     /gene="rpsQ"
                     /locus_tag="PLM92_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HPR24096.1"
                     /translation="MTDERKRRKTRVGIVVSDKMDKTAVVALENFVRHSLYGKSVKTT
                     KKVKAHDENNECNIGDKVRIMETRPLSRDKRWRLVKILEKVK"
     gene            complement(5529..5735)
                     /gene="rpmC"
                     /locus_tag="PLM92_02780"
     CDS             complement(5529..5735)
                     /gene="rpmC"
                     /locus_tag="PLM92_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005999409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HPR24097.1"
                     /translation="MELKKIREMTDLELNAELAKMKKDLFNLRFQHVTGQLENPIKMR
                     DTKRDIARVKTIIREKELKQAQSE"
     gene            complement(5735..6175)
                     /gene="rplP"
                     /locus_tag="PLM92_02785"
     CDS             complement(5735..6175)
                     /gene="rplP"
                     /locus_tag="PLM92_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005838599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HPR24098.1"
                     /translation="MLMPKRVKRRRVHRGRMKGKATRGNKVTYGEYGLVATEPAWITS
                     NQIEAARIAMTRSIKRGGKVFIKIFPHKSVTKKPAEVRMGSGKGAPEYWVAVVKPGRV
                     MFEIEGVTENQAKEAMRLASHKLPIKCKFVAKGKEQQVAEGGEA"
     gene            complement(6451..7224)
                     /gene="rpsC"
                     /locus_tag="PLM92_02790"
                     /pseudo
     CDS             complement(6451..7224)
                     /gene="rpsC"
                     /locus_tag="PLM92_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009346079.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
     gene            complement(7225..7578)
                     /gene="rplV"
                     /locus_tag="PLM92_02795"
     CDS             complement(7225..7578)
                     /gene="rplV"
                     /locus_tag="PLM92_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009644447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HPR24099.1"
                     /translation="MEAKAIAKYVRMSPIKLQPVTDLVRGKDLKEALSILKFTPGKGA
                     VLIEKVVKSAAANAENNLEMNPDDLYVAEIYAHQGPTMKRWKAGAQGRASMILKRSSH
                     IGVTLKDRNSETEVQ"
     gene            complement(7598..7852)
                     /gene="rpsS"
                     /locus_tag="PLM92_02800"
     CDS             complement(7598..7852)
                     /gene="rpsS"
                     /locus_tag="PLM92_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005999401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HPR24100.1"
                     /translation="MLKAIEEMNAANEKRVIKTWSRPSTIFPQMVGHTIAVHDGRKHV
                     PVYITEDMVGHKLGEFAPTRTYRGHAADKKVRTTDPSKTV"
BASE COUNT         1582 a         2040 c         1385 g         2846 t
ORIGIN      
        1 tgagcagtga agtccctccg aactgtacgt caagcggagt tatctgtcct actatggtgg
       61 gaagagtcgc cactgccgcc aggaatacag ctcctacaaa agttatcctg gtcattactt
      121 tattcaaata ctctacggtg gtccttcccg gtcttatgcc gggaataaag cctccgttgg
      181 ccttcatatt atttgctact tccattggat tgaaagtgac agcagtataa aaatatgtaa
      241 agaatattat aagaactaca ttaaatactg catataccca tatccccggt gagtccacag
      301 gagaaagcca cttttctatc cactgggtaa aatcagtatc tgtacctgca aaatatgtta
      361 cagtcagagg gaattgaagc atcgaaagtg cgaatataac aggtattact cccgcctgat
      421 tgactttgag aggaatatgc gttgactgtc cgccgtacat tttccttccc acgactctct
      481 ttgcatactg taccggtatc cttctctgtc catgctgtac ttctattatt cccacaataa
      541 ctattattgc aaaaataacg aaaagtgcta tggtgagaaa tccgatctct cctgcctgat
      601 attttgtaag tgtctctctt atggcactgg gcagtctggc cactatccct gcaaatataa
      661 taagggatat gccgttgccg atgccgtttt cattgatttt ttctcccagc cacataagaa
      721 aagccgttcc ggcagacagc acaataacta ttactgccac cgagaaaaaa tctgtgctta
      781 ttagagcctg cctgaacatg cccaccgaaa cacctatggc ctgtacaaag gccagtgcca
      841 cagtcaggta tctggtgtat tgagttattt ttttcctgcc ttctgtccct tccctggaaa
      901 gtctttcaag ggcaggtatt gctatggtca gtaactgcag tattatcgat gcagtaatat
      961 atggagtgat actcaaagca aatatggtca tattgctgaa agatcctccc gaaaaaagat
     1021 caaacagctc cagaagtccc gtccctccct ggaatgcctg gtttaaaatt tctctgtcta
     1081 taccgggcac agggatgttt gaacctattc tgaaaactac cagcataaga agagtgaata
     1141 ttatttttct tcttatatcc tctatcttcc aggcttttgc aatggtcttc agcatctcca
     1201 tattaaatca cctctgcctt tcctccggca gactctattt tttccacagc ggtcttgctg
     1261 aacttgtgag ccctgacagt aatatttttt tccagtttcc cgttgccaag gatctttatc
     1321 ccgtctttta cttgtttgac aagtcctgat tcggcaagca tttcaggtgt tacaacagta
     1381 ccggcatcaa atctgttgag tgcatcaaca ttaagctctg catacttggt cccccaaata
     1441 tttgtgaatc ctctcttggg gagcctcctg taaagcggca tctgtccgcc ttcaaatcca
     1501 agtcttactc ccccgccact cctggagttc tgtccgttca tgcctcttcc ggcggtagtt
     1561 ccctgtccgg tggcatgacc gcggcctttc ctttttccgg tctttgcaga tccgggagct
     1621 gcttttaatt cgtgtaattt catatttttg gcacctccat tcctacagtt cttccaaagt
     1681 aacaagatgc ttaacttttt caacagcgcc ccttgttacc gggctgtcaa ctagctctac
     1741 agactggtgc atctttttta atcccagtgc ttctactact tttctctgtt tcggagaagc
     1801 gccgatggta ctttttgtca aagtgatttt tatcattttt gccatttgcc tctcctccta
     1861 tccgaatatt tcttcttttg tctttcctct gagtttgctt attttttcaa cagtcatcag
     1921 gtttttcagc ccttccatcg tggcataggc catattacct gtattatttg aacctattga
     1981 cttggctctt acgtctttta ccccggccga ttcaagcact gtacgtactg aagaacctgc
     2041 aattactccg gtaccctctg ctgccggcat gatcagaaca cgtcccgctc caaagactcc
     2101 caggatccta tggggtatag ttgtccctac agtggggaca tttataacat ttttctttgc
     2161 atcttctgta gcttttctaa cggcctcagg gatctcctga gccttaccga gacctactcc
     2221 cacatgacct tcaccgtctc cgacgactac agtaacgctg aatctcatgt tacggccgcc
     2281 tttaacggtt ttagcgacac gtctgatatt cacgatggtc tcttttaagt ccagccttga
     2341 tacatcaatt gtatttcgca ttgcccttcc tcctgttaga attttaatcc ggcttcacgt
     2401 gcaccgtctg ccagctcttt tactcttcca tgataaaggg atccgcctct gtcaaaaaca
     2461 acagtttcta ttcccttttc aagagctctc tttgcaatag cttctccgac tttctttgcg
     2521 gcttctttgt tcccgccgta ttttatatca tttttcagtc ctttgtccag ggatgaagcc
     2581 gccacaagtg tattgccgtt tacatcatcg attatctgaa cagatatatt cttattgctt
     2641 ctgaaaacac aaagtctggg tttttcagga gtgccatgca aattctgtct ggttcttcta
     2701 tgtcttgcta tacgtctttc atttctgctt tcttttgcca ttagttttca ctcctttcta
     2761 ctctgacgcc ttcacgccgg cttttccttc ttttctgcgt acatattcgc cctcataacg
     2821 gatgcccttg cctttgtatg gctccggcct cctccaggct cttatatcag ccgcataatt
     2881 gcccaccgcc tgcttgctga tcccttttac gattattttt gtcgcttcgg ggacttcagt
     2941 ggttatttct ttcgggtctt ccagttctac cggatgagag tatcctacat tcaatacaag
     3001 cttgtttcct tttttctcag ccttatatcc gactccctga agctcaagct tcttttcgaa
     3061 tccttttgtg actccctcga ccatatttga aatcaatgat cttgcaagtc cgtgctgtga
     3121 acggttttct ttttcatcgg aatctctttt gatgttcagg actccgtctt tttgttcgat
     3181 ctttataagt ttgctgatct tttcctgcaa ttctcccttg ggccctttaa ctgttacaac
     3241 gtttttttcg tcgactttta cttcaactcc ggcaggaaga tcaatgggtc ttattcctat
     3301 tcttgacatt ttcttttcct cctaccaaac ataacagatt acttctccgc ctaccccaag
     3361 ttttctggct tccttatcgc ttatcactcc gtttgaagtt gaaatgatag caataccaag
     3421 tcctcccagt accctgggca catcacttgc cttggcataa accttaaggc ccggctttga
     3481 aatcttcttg attccgctaa taactttatc tttatcgggg ccgtacttca tttgtactct
     3541 tattgtcccc tgcttgtcgt cttctataac atcaaatcct ttgatatatc cttcctctgc
     3601 cagtataccg gcaattgact ttttgatttt tgaggcaggt atatcaaccg tttcatgtcc
     3661 tacagtattg gcatttctga ttcttgtcag catatctgct atcggatctg tcattgtcat
     3721 atctgttgtt ctccttcctt ccgccagttt tgctacgcgc attttatgct tcacaacttg
     3781 acgaaaacgt taattttctt accaagaggc cttttttaca ccggggatct cgcccctgta
     3841 tgcaagctct ctgaagcaga tcctgcatat accgtatttc ttcagaacag aatggggtct
     3901 cccgcatatc ctgcatctgg tatatgccct tgtctgatac ttaggttttc tctgctgttt
     3961 aattttgaga gatgtctttg ccatgtttcc ctcctatttc tcaaacggca ttcccagaaa
     4021 tcccagaagc gctgccgctt cttcatctgt ttttgccgta gtggtgaata cgatgttcat
     4081 cccttttacc atatcgacct tgtcaaaatt gatttcaggg aatatcagct gctcttttat
     4141 tccaacagaa tagtttcccc ttccgtcaaa agcattcttg cttactcctt tgaagtctct
     4201 taccctcgga agagcgatgt tgaagaactt gtctgcaaat tcatacatcc cttcgcctct
     4261 caaagtgact tttgtgccca ccggcatgcc ctgtctgact ttgaagttag ctatggattt
     4321 cttggccttt gtgacaacgg gtctttgtcc tgcaataagg gccagttccg ctgttgccgt
     4381 ttccatggct ttcggattat ccttggcttc tccaagaccg atatttatgg ttattttgtc
     4441 aagctttggg acttccatga cgttctcata tttaaactgt tccatcatgg cagggaaagc
     4501 ttccttttta tatttttcct ttaatctggc tgtcaaaatc gtctcctcct ttcttattct
     4561 attactgttc cgcttttttt ggcaattctc acttttttgt cgccttccat tttatatccg
     4621 actcttgtcg gctcttttgt cttgctgtct atgagcatga catttgaaat gtccagcggt
     4681 ccttcctgat gttttatctc ggctgcagca gtccttgtct gcttctgatg tttggtctgc
     4741 atgtttatgc cttccacaag gaccttgttt ttctttggca ttgccttaag tactttgccc
     4801 tttttccctt tgtcttttcc ggtaaggaca atcaccatat catctttctt aatacgcatt
     4861 ttgtcctcct ataatacttc cggagccaat gagacgattt tcataaagct cttgtctctc
     4921 agctctcttg ccacgggtcc aaaaatacgg gtacccatgg ggtttttatc ttcttttatg
     4981 ataacagccg cattttgatc gaatttcaca taactgccgt caactcttct ggcaccatgt
     5041 ttggttctga caacaactgc tctcactact tcacccttct ttacaagacc gccgggtgtt
     5101 gcttccttta cggcacatac tattatatct ccgatattcg cgtactggcg gcgtgtcccg
     5161 ccaaggatac ggatgcataa aagttctttc gcaccggaat tgtcagctac tctgagtctg
     5221 ctctctacct ggatcatttt ctggtgcctc cttacttaac tttttctaat atcttgacaa
     5281 gtctccatct cttatcgcgg gaaagaggtc tcgtctccat tattcttact ttatcgccta
     5341 tattacactc gttgttttca tcatgcgctt tgactttctt tgtagtcttt actgacttac
     5401 cgtaaagaga atgtcttacg aaattttcaa gtgcaactac cgcagtcttg tccatcttat
     5461 cgcttactac tataccgact ctggtttttc ttctttttct ttcatctgtc ataattcatc
     5521 ctcctttctt actcgctttg cgcttgtttc agttcttttt ctctgataat agtttttact
     5581 cttgcaatgt ctctttttgt gtctctcatc ttgattggat tttccagctg ccctgttaca
     5641 tgctggaacc tcaggttaaa gaggtccttt ttcatcttgg ccagttcagc attcagttcc
     5701 agatctgtca tttctcttat ttttttcagt tccattatgc ttcaccaccc tctgctacct
     5761 gctgttcttt tccttttgcc acaaacttgc attttatggg gagtttgtga gatgccagtc
     5821 tcatggcctc ttttgcctga ttctctgtta caccttctat ttcgaacatt actctgcccg
     5881 gttttactac tgccacccaa tattcgggag cacctttacc ggaacccata cgtacttctg
     5941 ccggtttttt tgtcactgac ttgtgcggga agatttttat aaatactttg ccgcctcttt
     6001 tgatcgatct tgtcatggca attcttgctg cctcgatctg attagaagtg atccatgcag
     6061 gttctgtagc aaccagacca tattcaccgt atgttacttt gttgcctctt gttgccttgc
     6121 ctttcatcct gcctctgtgg actcttctgc gtttaacgcg ctttggcatt aacatggctg
     6181 tttcctcctt tcgctaccag tctattcttc agttttttca acagcttccg cttcagctga
     6241 tgatactgtt tctttttctt ttgatgctgc ttcagccgcc ttttcttccg ctgccgctgt
     6301 ctcagccgct ttttgttctg cttcggctga agcttttttc atttcttcag cttttgcctt
     6361 tgcttcggct tcttccttgg gatccggttt gggggagttc tttattctcg gatttacagc
     6421 ctttggcatt tcgggcctgt cgtctctgct tctccttctg tctcttcctt caccatctct
     6481 tctcggtctt ctgtcaccgt ctcttctcgg tcttctgtcg ccgtctcttc tgggttttct
     6541 gtcccctttt cttccatcgg ttttttcttc cctgacagtg ctctgaaggg ttttttctaa
     6601 tatttcacca tgatttatcc aaactttcac acctattttt ccgtaggtcg tatcagcttc
     6661 ggcaaaacca taatcgatat ccgctctcaa agtatggagg ggaacattcc cttcactgta
     6721 tttttcgttt ctggcgattt ctgcaccgcc cagtcttccg gaaaccaaaa cctttacgcc
     6781 ttttgctcct gctttaatgg tcctgcccat tgcctgcttc atggcccttc tgaaagctat
     6841 tcttttttcc agtgcctggg ctatgctttc tgctgtaagc tgagcatcca gttcagctct
     6901 tttgacttct ttgatatcga tggaaaccct tttcttcgta attttttcaa gttctttctt
     6961 gagcttttcg ataccgtctc cggatctgcc aattaccata ccgggcttgc ctgtcagcac
     7021 gatgaccctc atctggtttt tctcagttct ctcaattact actcttgaaa cgccggctgc
     7081 atataatttt ttcttcacat attctcttat ttcgttatcc tcaacaagat aatccgaaaa
     7141 gattttttta tctgcatacc actttgaatc ccagtctctg attattccaa ctctgagacc
     7201 gtgtggactt actttctgac ccatttactg aacctccgtt tctgaatttc tgtcctttag
     7261 agtaacgcct atatggctgc ttctcttgag gatcatcgat gctctgccct gtgctccggc
     7321 tttccatctt ttcatagtgg gaccctggtg agcatatatt tctgccacgt acaaatcatc
     7381 ggggttcatc tcaaggttgt tttctgcatt tgccgcagcg gacttaacaa ccttttcaat
     7441 cagcacagca ccttttcccg gtgtgaattt cagtatactc agtgcctctt tcaaatcctt
     7501 gcctcttacc agatctgtaa caggctgcag tttgatagga gacatcctga catatttagc
     7561 aattgctttt gcttccattt tcaattatct cctttcatca aactgttttt gagggatctg
     7621 tagttctgac ttttttgtca gccgcatgac ctctgtaagt tcttgtcgga gcaaattctc
     7681 cgagtttgtg ccctaccata tcttccgtta tatatacggg aacatgtttt ctgccgtcat
     7741 ggacagctat tgtatgtccc accatttgcg ggaatatagt tgatggtctt gaccatgttt
     7801 tgattactct cttttcgttg gctgcgttca tctcttcgat tgctttgagc agc
//
LOCUS       DAONHK010000045         7802 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_7189_length_7802_cov_5.518911, whole genome shotgun sequence.
ACCESSION   DAONHK010000045 DAONHK010000000
VERSION     DAONHK010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 7802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7802
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7189_length_7802_cov_5.518911"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1995
                     /locus_tag="PLM92_02805"
     CDS             <1..1995
                     /locus_tag="PLM92_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPR24101.1"
                     /translation="KINKENGTIEDQAYLGQGVGVDYATIAPTLGEAKTGEEGNQITK
                     KIIMVPASRARLVAFDAETLDLLWTADPEAANDPENHRFDVDPQYDDNGQTISPVTYK
                     NGYAYYATWNGSGRPGYYYCVDTDNGEIKGYIKNQEGGFYWAGAYADPGIDSGADPGT
                     DPGYVAFGSQADSNGDATLYCMPSDLGGIGNDNESKPIDAELLEDSGNISGNVFYWED
                     NSEGIEYIFVMTDKSKLYKYEVEKDDTRKVTGISQTDPEEIYYLDLGGTISSGATIHD
                     GKIYAGVSGDSSGFGTSGHTLKIIDCESMTIDDSAPVPGFAKAEKLISIQNQNESGEL
                     YLYTTLNNMTGGIYLLKITDNSGTKEIEENNSGNLFVPDTQKQQYCISPLTADSEGNI
                     YYKNDSGNIMVVSEGYTITSSATSGGSITKTSGVIEGGNKDFEIAPAPGYISADIKVD
                     GISQGPASRYTFTNVTAKHSIKAVFIPAVTYGPAKIINISCSSVKLYWNPSANVTGYE
                     ILRAKAGGSFAKIADLKSTSTSYKNSGLSKGKKYSYKIRAYYSLSDGRKIYAGTSSVK
                     SITVKPAAPVIKTKTGKRKIKVSWKKVSGAKGYVVYRATKKAGKYKKIKTIKKSSARS
                     WTNKGLKKGKKYFYKVRAYTKVKKKTVRSNYSNISYKKAK"
     gene            2179..3519
                     /locus_tag="PLM92_02810"
     CDS             2179..3519
                     /locus_tag="PLM92_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPR24102.1"
                     /translation="MKKTKNKNIIAFIVSVAMVFTMFPAFASADASPVTAKVWFNASD
                     AERFIMPRQELTVSSDLSNEYYGIREQENGVTVIDVLIAACIKKYGNAFNDASKTNYL
                     DFSYSEAYDSYSVTKWFGQNTMNTLYYINRSMSLYSVDQSAVSNQDLIESVYVGESFS
                     DLYSCFDKNSYSVKTGRKLQITIKADTWGTAVKPNAGTLLCSVDRSTGSLTVLPGMVT
                     DAAGRALISFNKPGTYYISAKGPVTYDSFGSTVTGSIAAPWAEVKVTMEAPSVKAANK
                     SIKSIKISWKKITGAEKYQVYRSVKKNKGFKKVKTTKKTYWINTKLKTGKKYYYKVRA
                     ISGNEKSSFSNVKSAKPKPAKPVIRKIQNRGGNILISWKKVKDADGYVVYKKEGSKGK
                     FRNVKTLKASYSKQYKSMGINKGKVYSYKIKAYKIVKGKKVYGNFSGIKKIKAK"
     gene            complement(3664..4311)
                     /locus_tag="PLM92_02815"
     CDS             complement(3664..4311)
                     /locus_tag="PLM92_02815"
                     /inference="COORDINATES: protein motif:HMM:NF025338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPR24103.1"
                     /translation="MKTREKIKSTAIEMFNEKATSNISTVILSEKMGISPGNLYYYFE
                     NKEHIIRCIWEEDMLPENSKIFSADPSASSMEGIPEFLIGISDYFTRYRFFYLELFTI
                     LKNDPIMCKDYKKHIKKVRSQLQENFESWEESGIIDEIPEIQKHYLADDLLYAGFSSV
                     KMSYVINEKADLKELVRNSVMHMFFVLSGFLTESSKQKLKVLLEEKGFHFSTFGF"
     gene            complement(4321..5517)
                     /gene="alr"
                     /locus_tag="PLM92_02820"
     CDS             complement(4321..5517)
                     /gene="alr"
                     /locus_tag="PLM92_02820"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007059239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HPR24104.1"
                     /translation="MYKDAKRAAWAEINLSNADHNIKNIRKKVGPDVKITGIIKADGY
                     GHGAVAMAEVLRENNISSFGVATLHEAVALRDSGAKEEIVMLGLTPKMYSDTIVDYDI
                     TPVIMSHDNASAISDAAQKKGKVVSGFVAADTGMGRIGYPVSHLSAGKITAGELSAGT
                     PSAEYIFSIEDIRKIETLPCFKIKGLFSHFATADAKDKSFAREQEKNYNRFYDALLAS
                     GINIPMRTFANSAAIMELPSVWFDSVRPGIILYGCYPSHEVDKSLIDIKPVMSVKANI
                     VRLALMPEGASVSYGRKFIAKRESLIATLPLGYADGFPRPYSSVGRVLVNGVFAPIAG
                     NICMDQCMIDVTDVPGVKAGDEVIIIGSSGKNSILADDIGDATGTINYEIVCAFGQRL
                     PKVYIK"
     gene            complement(5510..6799)
                     /locus_tag="PLM92_02825"
     CDS             complement(5510..6799)
                     /locus_tag="PLM92_02825"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008725343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HPR24105.1"
                     /translation="MVKAIVGANWGDEGKGKITDMLAHESDIIVRFQGGSNAGHTIIN
                     DYGKFALHLLPSGVFYDHTTSIIANGVALNIPYFAKEIKSLTEKNVPAPKVLVSDRAQ
                     ILMPYHILFDQYEEERLGIKSFGSTKSGIAPCYSDKYAKIGFQVNELFSDTSFLREKA
                     ENVCLQKNPLLKNLYCKPELDPDDIVNTIRDYAEIIKPFVCDTSSFLHDAIKEKKNIL
                     LEGQLGALKDTDHGIYPMVTSSSTLAGFASVGAGIPPYEIKDIITVVKAYSSAVGAGA
                     FVSELFGKEAEELRKRGGDSGEYGATTGRPRRVGWFDAVATKYGCNIQGATEVALTVL
                     DPLGYLEQIPICIGYEIEGRTTKKFPNTTLLSRAKPILKTLPGWKCDIRGIREFNNLP
                     AECQNYVNEIEKELQLPVTLVSNGPGRSDIIRRKPDV"
     gene            complement(6843..>7802)
                     /locus_tag="PLM92_02830"
     CDS             complement(6843..>7802)
                     /locus_tag="PLM92_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HPR24106.1"
                     /translation="TTVGKRTTLWMQDIISDIHDLDYVLGSYMLLGSKGTTGTQASFL
                     ELFDGDHEKCKKLDLLVAKKMGFQAVFPVSGQTYPRKTDSRILNVLSGIAQSAHKFSN
                     DIRLLQHLKEIEEPFERQQIGSSAMAYKRNPMRSERIAALANYVVTDAMNPAITASTQ
                     WLERTLDDSANKRISISEAFLGVDGILDLYCNIASGLLVYPKIIEKHLKAELPFMATE
                     NILMDAVKKGGDRQELHEKIRMHSMEAARTVKEKGLPCDLAFRISKDEAFGMSEKEIN
                     AVMDPKNFIGCAKEQTANYLKNIVRPLLNKNRKLLGIKSKIEI"
BASE COUNT         2451 a         1551 c         1691 g         2109 t
ORIGIN      
        1 aaaataaata aagaaaacgg aactatagaa gaccaggcat atttgggaca aggagtggga
       61 gttgattatg caactatagc accgaccctg ggagaagcaa agacaggaga agagggaaat
      121 caaattacca aaaaaattat tatggtgcct gcaagccggg caaggcttgt ggcatttgac
      181 gccgaaaccc tggatcttct ctggacagcc gaccctgaag ctgccaacga tcccgaaaat
      241 catagatttg atgtggatcc gcaatatgat gacaacggac agacgatttc acctgtgaca
      301 tacaaaaacg gctatgcata ctatgccaca tggaacggtt cgggaagacc cggatattat
      361 tattgcgtag atacagacaa cggcgaaata aagggatata taaaaaatca ggagggtgga
      421 ttctactggg caggagcata tgcggatccg ggaatagatt ccggagcaga ccctgggaca
      481 gacccgggat atgtagcgtt cggatctcaa gcagacagta acggtgacgc tactctttac
      541 tgcatgcctt ccgatcttgg aggtatcgga aatgacaatg aatcaaaacc catagatgcg
      601 gagctacttg aagattcggg aaatatttca ggaaatgtgt tttattggga agataacagt
      661 gagggcatag aatatatatt tgtcatgact gacaagtcaa agctctataa atatgaagtt
      721 gaaaaagatg atacgagaaa agtcaccgga ataagtcaaa ctgatccaga ggagatttat
      781 tatctcgatc ttggcggcac catatcatcg ggagctacca tacatgacgg aaagatctat
      841 gcaggagttt caggggactc ttcgggattt ggaaccagtg gacacacttt gaaaataata
      901 gactgtgaaa gcatgacgat tgatgacagt gctcccgtac cgggttttgc aaaagcggaa
      961 aaactgattt ccattcaaaa ccaaaatgaa agcggcgaac tgtatttgta taccacatta
     1021 aacaatatga ccggcggcat atatcttctc aaaatcacag acaatagcgg tacaaaagaa
     1081 atagaggaaa ataactcggg aaaccttttt gttccggaca cacaaaagca gcaatactgt
     1141 atttctcctt tgaccgctga cagtgaggga aatatttatt acaaaaacga ctcgggaaat
     1201 ataatggtgg tcagtgaagg atatacaata acttcttcgg caacatccgg aggaagcata
     1261 acaaaaacaa gcggtgtcat agagggagga aacaaggatt ttgaaattgc ccctgcccca
     1321 gggtatattt cagcggatat aaaggtggat ggaataagcc agggaccggc aagcagatac
     1381 acttttacaa atgtcactgc taaacatagc ataaaggcgg tttttatacc tgctgttact
     1441 tacggacccg caaaaataat caacatcagc tgcagcagtg taaagcttta ctggaatcca
     1501 tctgcaaatg ttaccggcta tgagatccta agagcaaagg caggaggaag ttttgcaaaa
     1561 attgcagact tgaaatcaac aagcacatct tataaaaaca gcggcctttc caagggaaaa
     1621 aaatattcct ataaaatcag agcatattac agtttgtcag acggcagaaa aatatatgcc
     1681 ggtacttctt cggtaaagag cataacagta aagcctgcgg caccggtgat caagacaaag
     1741 acaggcaaaa gaaaaataaa agtaagctgg aaaaaggtat ccggagcaaa aggatatgtt
     1801 gtttacagag ccaccaaaaa agcaggcaaa tataaaaaga taaaaaccat aaaaaagagt
     1861 tcggcaaggt catggacgaa caagggattg aaaaaaggaa aaaaatattt ttataaagtc
     1921 agggcttaca caaaagtgaa aaagaaaact gtaagaagta attactcaaa tatcagctat
     1981 aaaaaagcca aataaaaaag acaaattaac agaaaccaat aagtaggcga aacagcaaaa
     2041 ccagaggaag aggcaaaata agaggagcgg caaaagcaga gaatcaggaa aaccggggga
     2101 acagcaatac cacggaggga agcagtaaaa aagcaccaaa ataatgaaga agaaacgtaa
     2161 aagaaagaga ggagaaaaat gaaaaaaaca aaaaacaaaa atatcatagc gtttatagta
     2221 tctgttgcta tggttttcac aatgtttccg gcttttgcat cagcggatgc cagcccggtc
     2281 accgcgaagg tatggttcaa tgcctccgat gcagaacgtt ttattatgcc gaggcaggag
     2341 ctcactgttt cgtccgatct ttccaacgaa tattatggca tacgagagca ggaaaacgga
     2401 gttacagtaa tcgatgtcct tatcgcagcc tgtataaaga aatacggaaa tgcattcaat
     2461 gatgcttcca agacaaacta tcttgatttt tcctacagcg aagcatacga ttcatattct
     2521 gtcacaaaat ggttcggtca aaacacaatg aacacgcttt attatatcaa cagaagcatg
     2581 tctttataca gtgttgatca gtctgctgta tcaaaccaag atctgatcga atcagtttat
     2641 gtgggagaat cattttcaga tctttattca tgctttgaca aaaatagtta cagtgtaaaa
     2701 accggccgga aattgcagat cacaataaag gctgatacct ggggaacagc tgtaaagccc
     2761 aatgcaggaa cacttttgtg cagcgttgat agaagcacag gctccctgac tgtacttccg
     2821 ggaatggtaa ctgatgccgc tggaagagca ttgatttcat ttaataagcc gggaacttac
     2881 tatatatcag caaagggacc agttacatat gacagttttg gttcaactgt gaccggcagc
     2941 atcgcggccc catgggcaga agtaaaagtg acaatggagg ctccttctgt aaaagcagca
     3001 aataaaagta taaaaagtat aaaaatcagc tggaaaaaaa ttaccggagc agagaaatac
     3061 caggtataca gatcagtaaa aaagaacaaa ggattcaaga aggtcaaaac aacgaaaaaa
     3121 acctattgga tcaacacaaa attaaaaacc ggaaagaaat attattacaa agtgcgggca
     3181 ataagcggaa atgaaaaaag ctctttttca aacgtaaaaa gtgcaaagcc aaaaccggca
     3241 aaaccagtaa taagaaagat acaaaacaga ggcggcaata ttctgataag ctggaaaaaa
     3301 gtaaaagatg cagacggata tgttgtctat aaaaaagaag gcagcaaagg caagttcaga
     3361 aatgtaaaaa cacttaaggc atcatacagc aaacaataca aaagcatggg aataaataaa
     3421 ggaaaagtat acagttataa gataaaggct tacaagatcg tgaaaggaaa aaaagtatat
     3481 ggtaattttt ccgggatcaa aaaaataaaa gcaaaataaa aatgcgtaaa ggcaaaagct
     3541 tttcatcctg aaagtgcggc agactgtaaa atccggtttg ccgtgctttt ttgactgaaa
     3601 atcaaaaatc tgccgcgaaa aacgaagagc ctattgacaa tactgaatgc cgtttttcca
     3661 aaatcaaaag ccgaatgtgg aaaaatgaaa tcctttttct tcgagcaaga cttttagttt
     3721 ctgtttagaa ctttcagtca aaaaccctga cagaacaaaa aacatatgca ttacagaatt
     3781 tctgacaagt tctttcagat ccgctttttc gtttattaca taagacattt ttacagaaga
     3841 aaatcctgca taaagcagat cgtctgcgag atagtgtttt tggatctcag ggatttcatc
     3901 tattattccc gattcttccc agctctcaaa attttcctga agttgggaac gtactttttt
     3961 gatgtgtttc ttgtaatctt tacacatgat gggatcgttt ttcaggatag taaatagctc
     4021 aagataaaag aaacgataac gggtaaaata atcggagatg ccaatcagaa attccggaat
     4081 gccttccatt gaagatgctg aaggatcagc tgaaaaaatc ttgctgtttt ccggaagcat
     4141 gtcttcttcc cagatacagc gtattatatg ctctttgttt tcaaaatagt aataaagatt
     4201 gccgggactt atgcccattt tttctgaaag gataacagta cttatattag atgtagcttt
     4261 ttcattgaac atctctatgg cagtactttt tattttttct ctggttttca taaaaacccc
     4321 ctattttata tagaccttcg gaagcctttg tccgaaggca cagactattt cgtagttgat
     4381 agttccggtg gcatcgccta tatcatcggc aagaatgcta tttttcccgc tacttccgat
     4441 tattatgact tcatctcctg cttttactcc gggaacatct gtaacgtcga tcatgcactg
     4501 atccatacat atgttgcctg ctatgggagc aaaaactccg tttacaagaa cccggcccac
     4561 tgaagaatag ggacggggaa atccgtctgc atatcccaga gggagggtgg ctataagact
     4621 ctctcttttt gcaatgaatt ttctcccgta acttacggaa gctccttcgg gcataagagc
     4681 cagccttact atattggcct ttacagacat tacaggtttt atatcaataa gacttttatc
     4741 aacttcgtgg gaaggatagc acccgtaaag aattatgccg ggacggacag aatcaaacca
     4801 cactgaaggc agttccataa tggcggcact gttggcaaat gtgcgcatgg gaatatttat
     4861 cccggaagcc agaagagcat cgtaaaatct gttatagttt ttttcctgtt ctcttgcaaa
     4921 agatttgtct ttggcatctg cagtggcaaa atgggaaaaa agacctttga tcttgaagca
     4981 gggaagagtt tcgatctttc ttatatcttc gatagaaaaa atatactcgg cagaaggagt
     5041 accggcagaa agctcgcccg cagtgatctt tccggcagaa agatggctga caggataacc
     5101 tatgcggccc atgcctgtat ctgcggcaac gaagcccgag accactttgc cttttttttg
     5161 tgcggcatcg gaaatggcag aagcgttatc gtgtgacatt atcacggggg taatatcata
     5221 atcaacaatg gtatccgaat acatttttgg agtcagaccc agcatgacta tttcttcttt
     5281 tgcccccgaa tctcttaaag cgactgcctc atggagagtg gccacgccga aggagcttat
     5341 attattttct ctcagaactt cggccatggc aacagctccg tgtccatagc catccgcttt
     5401 tattatgccg gtgatcttaa catcaggacc gacttttttg cggatatttt ttatgttatg
     5461 atctgcgttt gaaaggttga tctccgccca ggcggcacgt tttgcgtctt tatacatcag
     5521 gttttctcct tatgatgtcg ctgcggcccg gtccgttgga caccagagta acggggagct
     5581 gcagttcttt ttctatctca tttacatagt tttggcattc agccggaaga ttgttaaatt
     5641 ctctgatacc gcggatatca catttccatc ccggaagggt tttaagaatg ggctttgccc
     5701 gggacaaaag agtggtattg gggaattttt ttgtggttct gccttctatt tcatagccga
     5761 tgcagatggg tatttgttca agatatccca gcggatcaag gacagtcaga gcgacttccg
     5821 tggctccctg gatattgcat ccgtattttg tggcaacagc atcaaaccag cccactcttc
     5881 tgggtcttcc ggtggttgca ccgtattccc cgctgtctcc tcctcttttt ctcagttctt
     5941 ctgcttcttt accgaagagc tcacttacaa aagcgcccgc tccgacggca ctggagtaag
     6001 cctttacaac ggttatgata tcctttattt catagggagg gatacccgca cccactgagg
     6061 cgaaaccggc cagggtggaa gaagaagtga ccattgggta tatgccatga tccgtatcct
     6121 taagggctcc cagttgtcct tctagaagga tgttcttttt ttcttttatt gcatcatgga
     6181 gaaaggaaga agtatcacat acgaagggct ttattatttc ggcataatcc ctaattgtat
     6241 ttactatatc atcggggtca agctccggtt tgcagtaaag attttttaaa aggggatttt
     6301 tttgaaggca tacattttct gctttctccc tgagaaaaga cgtgtctgaa aaaagctcat
     6361 ttacctggaa gcctattttt gcatatttgt ccgaataaca tggagcgata cctgactttg
     6421 tggagccaaa agattttatt cccagtcgtt cttcttcgta ctggtcaaaa agtatatgat
     6481 atggcataag tatctgggct ctgtctgaaa ccagcacctt tggcgccgga acattttttt
     6541 ctgtaagaga ttttatttct tttgcaaagt aaggtatatt cagagccaca ccgtttgcta
     6601 tgatgctggt ggtatgatcg taaaaaaccc ctgacggaag aaggtgcaga gcgaactttc
     6661 cgtaatcgtt tattatggta tgtcccgcat tgcttcctcc ctggaaacgc actatgatat
     6721 ctgattcatg ggcaagcatg tctgttattt ttcccttgcc ctcgtctccc cagtttgctc
     6781 ctacgattgc ttttaccatt gcaaaagttc ctttcgtttt atatttctat cttgggtccg
     6841 atctatattt ctattttgga cttgatgccc agtaactttc tatttttgtt caaaagagga
     6901 cggacgatat ttttgaggta gttggcagtc tgctctttgg cacagcctat gaaatttttc
     6961 ggatccatga ctgcatttat ttctttttca ctcatgccga aggcttcgtc ttttgatatg
     7021 cgaaaagcca ggtcgcaggg aagccctttt tctttgaccg tcctggccgc ttccatggaa
     7081 tgcatcctta ttttttcgtg aagttcctgt ctgtcaccgc cttttttcac tgcatccata
     7141 aggatatttt cggtggccat aaagggcagt tccgctttca gatgtttttc tattatcttt
     7201 ggataaacca aaagtcctga agctatattg cagtaaagat caaggatacc gtcaacacca
     7261 agaaaggctt cggaaatact tatccttttg ttggcggaat catcaagggt gcgttcaagc
     7321 cactgggtgg aagcggttat ggccgggttc atggcatctg ttacaacata atttgcaaga
     7381 gctgctatac gctcacttct catggggttc cttttgtatg ccatggcgga tgatccgatc
     7441 tgctgtctct caaagggttc ttctatttct ttcagatgct gcagcagtct tatatcattg
     7501 gaaaatttgt gagcactttg agctatcccg ctcagaacat taaggatacg gctgtcggtt
     7561 ttacgtggat atgtttgacc cgacacaggg aaaactgcct gaaagcccat ttttttggca
     7621 accagcagat ccagtttttt gcacttctca tgatcaccgt caaagagttc aagaaaactg
     7681 gcctgtgtgc ctgttgtacc ctttgagccc aaaagcatat aggatcccag tacatagtca
     7741 agatcatgta tgtcacttat gatgtcctgc atccaaaggg tggtcctttt tcccacggtt
     7801 gt
//
LOCUS       DAONHK010000046         7743 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_7269_length_7743_cov_4.391649, whole genome shotgun sequence.
ACCESSION   DAONHK010000046 DAONHK010000000
VERSION     DAONHK010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 7743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7743
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7269_length_7743_cov_4.391649"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..549
                     /gene="frr"
                     /locus_tag="PLM92_02835"
     CDS             <1..549
                     /gene="frr"
                     /locus_tag="PLM92_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016626710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HPR24107.1"
                     /translation="KVIEVLETKIGKTESALKEELNAVRAGRANPALMDRIEVEYYGT
                     PTPLKQLANISVPDPRTLMITPFDPKSIGDIEKAINIANLGISPANDGKCIRLAIPQV
                     TEERRKELTKTVKSMGEEAKVAVRNLRRTANDELKKEEKAGDLTEDDLKEDLDEVQKK
                     VDDAIKAIDEILAEKEKEIMEV"
     gene            556..1278
                     /locus_tag="PLM92_02840"
     CDS             556..1278
                     /locus_tag="PLM92_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009644768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HPR24108.1"
                     /translation="MLDMKRIPAHVAIIMDGNGRWAKEKNLPKLFGHNAGMKAMKEIV
                     KRASALGIGYLTVYAFSTENWKRSEEEVSGIFKLLVKYVDKELDELNKNDVKVNILGD
                     HSYLPSQARERIEKAVCYTSDNGGLNFNIALNYGSRDEITKAVCKIAEMVEKKEIRLC
                     DITEDMISDSLFTGESKIPDPDLLIRTSGEKRLSNYLLWQLAYSEFVFTDTYWPDFSP
                     EEFEKAIEEYQNRDRRFGGRRK"
     gene            1275..2081
                     /locus_tag="PLM92_02845"
     CDS             1275..2081
                     /locus_tag="PLM92_02845"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009644736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HPR24109.1"
                     /translation="MKTRIISGLIMLPMLAVLYFGGYVLMAACFGLTLAALYEFFKGF
                     HSMGVKPSYPVASVSVMLLYGYTLYLNLSGGNVIDLSGPFMFWVFITLTASLIYLFRI
                     EKRQLQDAMATVTGVLYIAFFAFHLVLVDQTGEPSILIWIIIFSAFGTDVFAYFTGLA
                     LGRHKLCPSISPKKTVEGAVGGVIGSIIICGAFGYFFAEEYLSHCLILGIMGGIISQL
                     GDLTASVFKRKMGIKDYGHLIPGHGGILDRFDSVLFTAPMVYYYIIFVIQ"
     gene            2100..3227
                     /gene="dxr"
                     /locus_tag="PLM92_02850"
     CDS             2100..3227
                     /gene="dxr"
                     /locus_tag="PLM92_02850"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013150327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HPR24110.1"
                     /translation="MKKIAVLGSTGSIGTQALEIIEKNREEYSVSALACGKNISLLQK
                     QIDLFLPDMVSVKEKKDAEELQKKYPKIHIVHGGEGLIEAATGSCHTVLNALMGIKGL
                     MPTYRAILAGKDIALANKETLVAGGQLIMEKAAEKGVEILPVDSEHSAVFQCMQGNEK
                     KSVKRIILTGSGGPFRGFSMGQLDAVTKEQALAHPNWSMGQKITIDSATLMNKGLEVI
                     EARWLFDVALEKIDVVIHPQSIIHSMVEYEDNSVMAQMGIPDMKVPIAYALSFPKRRK
                     NDFKSLDLIREGANLTFESPDTNTFRCLSLAYEASKKGGTYPVVTNGANEVLVQRFLE
                     GEIGFTQIQDTIERVLNEHIPKYRLTPEMILEEDKKIREKI"
     gene            3224..4237
                     /gene="rseP"
                     /locus_tag="PLM92_02855"
     CDS             3224..4237
                     /gene="rseP"
                     /locus_tag="PLM92_02855"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HPR24111.1"
                     /translation="MITVIYAVAMFCVLIFMHELGHFIAARACNVKVNKFAIGMGPAF
                     WKKQKGETEYSLRIFPIGGYCLMEGEDEDSDEPRALNNKKAWQKAIVICAGSFMNLLL
                     AVILMIIFVFYIGTATTTLDTVQKDSPAEEAGLKPGDKIVMVDGKKTNEWTDVTDIIT
                     GTEKNKAEITVERNGKEYTFVTGFMKSEEGRKIIGITSARQREPVSALKDGVKATFDM
                     TLNMYEILKQLFTGDVSAKELSGPVGIVYMVDQSVSYGFMYFLYFMSLISLNLAIVNM
                     LPLPALDGGRLLFIIVRKITGKAITDDMEGKIHFIGIMLLLLLMVYVTWNDIIRFIVP
                     IFN"
     gene            4261..5331
                     /gene="ispG"
                     /locus_tag="PLM92_02860"
                     /gene_synonym="gcpE"
     CDS             4261..5331
                     /gene="ispG"
                     /locus_tag="PLM92_02860"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018594298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HPR24112.1"
                     /translation="MTKRVQCGNIYIGGGAPVSVQSMTNKDTRDIKACISQIERLADT
                     GCHIVRIAVFDTEAAEAFGEIKKATDVPLVADIHFDHRLALAAIEKGADKIRINPGNI
                     GSKEKIRQVIGAAKKRGIPIRVGVNSGSLEKDILEKYKGVTAEGLAESAIRSAKLLED
                     MDFEDIVLSVKASDPAINYDACMLLSKMTKYPMHIGVTEAGTQEQGIIKSAVGIGALL
                     LAGVGDTVRVSLTGDPKAEVIAAKKIIAAAGRGRMPINIISCPTCGRSRMDIERLAVE
                     LEKEIMPLGEKRKKAGMKSLDIAVMGCEVNGPGEAKEADFGVACGKGKGLIFIRGKAL
                     KTVPEEEIIKELVRMAENYENI"
     gene            5318..5746
                     /locus_tag="PLM92_02865"
     CDS             5318..5746
                     /locus_tag="PLM92_02865"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HPR24113.1"
                     /translation="MKTYNATHMIRYEDLNHHANLYAGRASEWMIEASFAAVTIVYGN
                     GDGLRYKNTHKFDFRKSVYPGDIITFAAKVVRMGKTSITVYVELIDEKTKEVKAESFV
                     TYVTVDNEGRPVGHGLGLDETHDEKEKKCRKEAESFFRKL"
     gene            5743..>7743
                     /locus_tag="PLM92_02870"
     CDS             5743..>7743
                     /locus_tag="PLM92_02870"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HPR24114.1"
                     /translation="MKNILDSYIREGYRYKVEKAVMTKETGSLTLYIRLNFIVPAEDI
                     DRIEEAIKKQIPAVSQTDIRFIYEDVRLERADIFDRFIGHMIRMSNGRYSAVTKVIYP
                     HEFREEGGHLIIYALGETAVRTLNRQVATEFQKMLKDNFDIDIKVDFKNHEDEYARTS
                     MDIESRGEESSKKPATKAKTVNKTKRNDKQDTIMGREIKEPVMSISGLPETGEAVIEG
                     RVFEITAKTVRNGKKLINILLYDGTTSACAKAFMKEEKWNDISEKLKENDLLKIKGDI
                     EWDNFSNNIVIMAKHIEKGNQTKREDLSESKRIELHAHTKMSAMDGFNDIRGMIEQAA
                     YWGHRAIAITDHGVVQAFPEAFKTAADLKKQTGHEIKIIYGLEGYIFDDTDCLLPDGQ
                     IDYRSKKTNHVIIIAKDQKGLKNLYRLVSESHIKYFYRKPRIPKSLLSDRREGLIIGS
                     ACAEGEVYQAVLRKYTKKQIDEIVNYYDYLEIQPLINNQFLIDGGLVSDKEELKDINR
                     RIIDLGREHGKTVVATCDTHYTSPDDFLYRKILMAGQGYKDTEGEQGLYLRTTEEMLS
                     EFDYLDEETAADLVIKGPGHIADMIEDILPVPFGKFPPKIEDADGILRRKCEENAKAR
                     YGDPLPDKIRERLFAELDSIIGNDYAVLYVSAEKLVQKSLSDG"
BASE COUNT         2654 a         1381 c         1881 g         1827 t
ORIGIN      
        1 aaagttatcg aagttctgga aacaaaaata gggaaaacag aatcagccct aaaagaagaa
       61 ctaaatgcgg taagggcagg aagagctaat cctgcgctta tggacaggat cgaagtagag
      121 tattacggta cacccacacc tcttaagcag ctggcaaata tatcggtccc cgatcccagg
      181 acactgatga tcacgccttt tgatcccaag tctatagggg atatcgaaaa ggccataaat
      241 attgcaaatc tgggaataag cccggcaaat gacggtaaat gtataaggct cgccattccc
      301 caggtcacgg aagaaagaag aaaagaactc acaaaaaccg tcaaatccat gggagaagaa
      361 gcaaaagtag cggtaagaaa cctcagaagg actgcaaatg acgagctcaa aaaagaagaa
      421 aaagccggag atcttacaga agatgactta aaagaagatt tagatgaagt tcagaaaaaa
      481 gttgacgatg ctataaaggc tattgatgaa atactggcag aaaaagaaaa agaaatcatg
      541 gaagtatgat ctgaaatgct tgatatgaaa aggatacctg cccatgttgc catcataatg
      601 gatggcaacg gcaggtgggc caaagaaaag aatctgccca aattattcgg ccataatgcc
      661 gggatgaagg caatgaaaga gatagtcaaa agagcttcgg ctctgggcat aggatactta
      721 accgtttacg cattttccac tgaaaactgg aaacgttcag aagaagaagt aagcggtatt
      781 tttaagttac tggtcaagta tgttgataag gaacttgatg aactcaataa aaatgatgta
      841 aaagtgaata tcctgggaga tcacagttat cttccctccc aggcgagaga aagaatagaa
      901 aaggctgtct gctacacatc ggacaatggt ggcctgaact tcaacatagc cttgaactac
      961 ggaagcagag atgaaataac aaaggcagta tgcaaaatag cggaaatggt cgaaaaaaag
     1021 gaaataaggc tctgcgatat aacggaggat atgatatcgg attccctttt taccggagaa
     1081 tcaaaaatcc ctgatccgga tctgcttata aggaccagcg gtgagaaacg tctgtcaaat
     1141 tatctgctct ggcagctggc atacagtgaa tttgttttta ctgatactta ctggcccgat
     1201 ttttcaccgg aagaattcga gaaagccata gaagagtatc aaaacagaga cagaaggttc
     1261 ggaggaagaa gaaaatgaaa acaaggatca tttctgggct gataatgctg ccaatgctgg
     1321 cggtcttata ttttggggga tatgtgctga tggcggcatg ttttggactt acccttgctg
     1381 ctctttatga attcttcaaa ggattccatt ccatgggagt aaagccttct tatccggtgg
     1441 cttcagtttc tgtaatgttg ctttatggct atacacttta tttaaatctt tcgggaggaa
     1501 atgtgatcga tctttccgga ccattcatgt tctgggtatt tattacgctc acagcaagcc
     1561 tgatatatct tttccggata gaaaaaagac agcttcagga tgccatggcc acggtcacgg
     1621 gagttttata tatcgctttc tttgcatttc atcttgttct ggtggatcag acgggagaac
     1681 cgtcgatcct gatatggata atcatatttt cagccttcgg taccgatgtg tttgcatatt
     1741 tcacaggcct tgctttgggc cggcataaac tctgcccttc tataagtccg aaaaaaactg
     1801 tggaaggtgc cgttggcggt gttataggca gcataatcat atgcggagct ttcggatatt
     1861 tttttgccga agaatatttg agccactgcc ttatacttgg tataatgggc ggtatcatat
     1921 ctcagctggg agaccttacc gcttctgtat tcaagagaaa gatgggtata aaggattacg
     1981 gccatctgat accgggtcac ggtgggatac ttgatcgttt tgacagcgtt ctttttacag
     2041 cgccaatggt ttattattat attatctttg taatacagta aaaggtatca tttataatca
     2101 tgaagaaaat agccgtgctg ggcagtaccg ggtcaatagg tactcaggca cttgaaataa
     2161 tagaaaaaaa cagagaagag tattccgtat cagcactggc atgcggaaaa aatatttctt
     2221 tgttgcaaaa acagatagac ttgtttttgc ctgatatggt ttctgtcaaa gaaaagaagg
     2281 atgcggaaga acttcaaaaa aaatatccga aaatccatat tgttcatgga ggagaaggcc
     2341 ttatcgaggc ggctaccgga tcttgccata cggtccttaa tgctcttatg ggcataaagg
     2401 gtctaatgcc cacatacaga gctatactgg caggcaaaga tattgcgctg gccaacaaag
     2461 agacccttgt ggcaggggga cagctcataa tggaaaaagc cgccgaaaag ggagttgaga
     2521 tcctgcccgt agacagtgaa cacagtgccg tattccagtg tatgcagggc aacgaaaaaa
     2581 aatccgtaaa aagaataata ctgacgggat cgggaggacc tttcagagga ttttccatgg
     2641 ggcagcttga tgcagtgaca aaagagcagg cccttgctca tcccaactgg agcatgggac
     2701 agaaaataac aattgattcg gcaactctta tgaacaaggg acttgaggtc atagaagcca
     2761 gatggctttt tgatgtggct cttgaaaaaa tagatgtggt tatacatccc cagagtataa
     2821 tacattcaat ggtagaatat gaggacaatt ctgttatggc acagatgggg atccctgata
     2881 tgaaggtgcc cattgcctat gcgctgagct tcccaaaacg acgcaaaaac gatttcaaaa
     2941 gcctggatct tatcagggaa ggagcaaacc ttacttttga gagccctgat acaaatacct
     3001 tccggtgcct gtctcttgca tatgaagcat caaaaaaagg gggcacatat cccgtggtaa
     3061 cgaacggggc aaatgaagtc ctggtacaga gattccttga aggggagata ggattcacac
     3121 aaatacagga tacgatagag agagtgctca atgagcatat accaaaatac cggctgacgc
     3181 cggaaatgat ccttgaggaa gataaaaaga taagggaaaa aatatgataa ctgtaattta
     3241 tgccgttgca atgttctgcg tgctgatatt tatgcacgaa ctcggtcatt ttatagcagc
     3301 aagagcatgt aatgtaaaag tgaataaatt tgccattggc atgggcccgg cattctggaa
     3361 aaagcagaaa ggcgaaacgg agtattcctt aaggatattc cccataggtg gctactgctt
     3421 aatggaggga gaagatgaag attcggatga accaagagcc ctgaacaaca aaaaagcatg
     3481 gcagaaggcc atagttattt gtgccggttc ttttatgaac cttttgctgg ctgttattct
     3541 tatgataata ttcgttttct atataggaac tgccactact accctggata cggtacaaaa
     3601 agatagtccc gctgaagaag ccggtctgaa gccgggagac aaaatcgtta tggtggatgg
     3661 caaaaagaca aatgagtgga cggatgttac agacataatc accggcacag agaagaacaa
     3721 agcagagata actgtcgaaa gaaacggcaa agaatatacc tttgtaacag gcttcatgaa
     3781 aagtgaagaa ggccggaaaa ttatcggcat aacatcagcc agacagagag aaccggtttc
     3841 tgccctaaaa gatggggtca aagcaacttt tgatatgacc ctgaacatgt atgaaatatt
     3901 aaaacagctt ttcacgggag atgtttccgc aaaagaactt tcagggcccg tgggcatagt
     3961 ttatatggtg gatcagagcg tcagttacgg atttatgtat tttctgtatt tcatgtcgct
     4021 cataagtctg aatcttgcca tagtcaatat gctgcccctg ccggcactgg acgggggacg
     4081 attgcttttt ataatagtaa gaaagataac cggcaaagct ataaccgatg atatggaggg
     4141 gaagatccat ttcataggaa taatgcttct gcttttgctg atggtatatg ttacatggaa
     4201 tgacattatc agatttatag ttcccatatt caactgataa cagattaagc aggtataacg
     4261 atgacaaaaa gagtacaatg tggaaatata tatataggcg gcggggctcc ggtctcggtg
     4321 cagtctatga caaataagga tacaagagat ataaaagcgt gcatttctca aatagaaaga
     4381 ctggctgata caggttgcca tatagtacgt attgcggttt ttgatacgga ggcggcagaa
     4441 gctttcggag aaataaaaaa agcaacagat gtgcctttgg tggcggacat acattttgat
     4501 cacagactgg cattggcggc catcgaaaaa ggtgcagaca aaataagaat aaatccggga
     4561 aacattggca gcaaagaaaa aatacgtcag gttatcggtg cggcaaaaaa aagaggaata
     4621 cccataagag ttggagtcaa ttccggttcc cttgaaaagg acatactcga aaaatataaa
     4681 ggagtaacag cagagggtct tgcagagagt gccatcagaa gcgcgaaatt attagaagac
     4741 atggattttg aagatatagt tttatcggta aaggcctcag acccggcgat caattatgat
     4801 gcatgtatgc tgctctcaaa aatgacaaaa taccccatgc atatcggagt gactgaagcc
     4861 ggaacacagg agcaggggat aataaagtca gcagtaggca taggagctct gctactggcc
     4921 ggtgtagggg acacagtgag agtttctctt acaggagacc cgaaagcaga agttatcgcc
     4981 gcaaaaaaga taattgccgc cgccggccgt ggaaggatgc ccatcaatat aatatcatgt
     5041 cccacatgtg ggcgcagcag gatggatatt gagagactgg cagtggaact ggaaaaagaa
     5101 ataatgccac ttggtgaaaa aagaaagaaa gcagggatga aaagtctcga tatcgcagtt
     5161 atgggatgtg aagtcaatgg ccccggagaa gcaaaagaag cggatttcgg tgttgcatgc
     5221 ggaaaaggaa aaggacttat attcatccgg ggcaaagcgt taaagacagt gccggaggag
     5281 gaaataataa aagaacttgt gaggatggca gaaaactatg aaaacatata atgctacaca
     5341 tatgataaga tatgaagacc ttaaccatca tgctaatctt tatgcgggaa gggcttctga
     5401 atggatgatt gaagcttcat ttgcagcagt caccattgtt tacggaaacg gagacggcct
     5461 gagatataaa aacacacata aattcgattt caggaaatcg gtttatccgg gagacatcat
     5521 aacctttgca gcaaaagttg taaggatggg caaaaccagt attacggtat atgtggagct
     5581 tattgatgaa aagacaaaag aagtcaaggc agaaagtttt gttacatatg ttactgttga
     5641 taatgaaggc agacccgtag gacacggact cgggcttgat gaaacacatg atgaaaaaga
     5701 aaaaaaatgt agaaaggaag cggaatcatt ttttaggaag ctatgaagaa catacttgac
     5761 agttatataa gagaaggata ccggtataaa gttgaaaaag cagtcatgac aaaagagaca
     5821 ggatctctga ctctttatat aagactcaat tttattgtcc ctgcagaaga tatagacaga
     5881 atagaagaag ccataaaaaa acagataccg gcggtatccc aaactgacat cagattcata
     5941 tacgaagatg tccgtcttga aagggcggat atttttgata gattcatagg ccatatgata
     6001 aggatgtcaa acggcagata ctctgctgtt acaaaggtga tatatcccca tgaattcagg
     6061 gaagagggag gacatctgat aatatatgct ctcggggaga ctgcggtcag gacactcaac
     6121 agacaggtgg caacggaatt tcagaaaatg ctgaaggata attttgatat tgatataaaa
     6181 gtggatttca aaaaccatga ggatgagtat gccagaacat caatggacat agaaagccgg
     6241 ggagaagagt cttcaaaaaa acccgcaaca aaggcaaaga cagtcaataa aaccaaaaga
     6301 aacgacaagc aggataccat aatgggaagg gaaataaaag aaccggtcat gtcaatcagc
     6361 ggtctgcccg aaacgggaga ggccgtaatt gaaggaaggg tttttgagat aacggcaaaa
     6421 accgtaagaa acggcaaaaa attgatcaac atacttttgt atgacgggac tacttcggca
     6481 tgtgcaaaag cttttatgaa ggaagaaaaa tggaatgata tatctgaaaa actgaaggaa
     6541 aacgatctgc tcaaaataaa aggggatata gaatgggaca acttcagtaa taatattgtc
     6601 ataatggcta aacatataga aaaaggaaac cagacaaaaa gagaagacct gtcagaatca
     6661 aaaagaatcg aattacatgc gcacaccaag atgagtgcca tggacggatt caatgatatc
     6721 aggggtatga tagagcaggc agcttattgg ggacacaggg ccatcgccat aacggatcat
     6781 ggagtggttc aggctttccc ggaagctttc aagacggcgg cggatctgaa aaaacaaacg
     6841 ggacatgaaa tcaaaatcat atacggactt gagggatata tttttgatga cacagactgc
     6901 ctgctgcccg atggacaaat cgactacaga tccaagaaaa ccaatcatgt gataataatc
     6961 gcaaaggatc aaaaaggtct gaagaacctg tatcgtcttg tttcggagtc ccatatcaaa
     7021 tatttttaca gaaaacccag gataccaaag tccctgcttt ccgacagaag agaagggctg
     7081 atcatcggtt cggcctgtgc agaaggagaa gtatatcagg ctgttctaag aaaatataca
     7141 aaaaaacaga tagatgaaat agtaaactat tacgattatc ttgaaataca gcctctgata
     7201 aacaatcagt ttcttataga cgggggtctg gtttcagaca aagaagaact taaggatata
     7261 aaccggagaa taatagacct gggcagggag cacggcaaaa cagttgttgc aacctgtgat
     7321 actcactata catctcccga tgattttctt taccgaaaaa tactcatggc ggggcaggga
     7381 tataaggata cagaaggaga gcagggactt tacctgcgca ccaccgagga aatgctttct
     7441 gagttcgatt acctggatga agaaacagct gctgatctgg tcataaaagg tcccggacat
     7501 atagcggata tgatcgagga catattgccg gtgccttttg gcaaatttcc gccaaagatc
     7561 gaagatgccg atggaatact acgcagaaag tgtgaggaaa atgcaaaggc aagatacggg
     7621 gaccctttgc ccgataaaat aagagaaaga ctttttgcgg aacttgattc cataatcgga
     7681 aatgattatg cagtgctata tgtttcagcg gaaaagcttg ttcaaaaatc tctttccgac
     7741 gga
//
LOCUS       DAONHK010000047         7541 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_7549_length_7541_cov_4.936281, whole genome shotgun sequence.
ACCESSION   DAONHK010000047 DAONHK010000000
VERSION     DAONHK010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 7541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7541
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7549_length_7541_cov_4.936281"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="PLM92_02875"
     CDS             complement(<1..239)
                     /locus_tag="PLM92_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein"
                     /protein_id="HPR24115.1"
                     /translation="MKEKKVVAKNRKARHDYFIEDTYEAGLVLTGTEIKAIRQGKVSI
                     KESYARIKNGEAFIHGMNISPYEQGNIFNVDPLRT"
     gene            complement(256..756)
                     /gene="lepB"
                     /locus_tag="PLM92_02880"
     CDS             complement(256..756)
                     /gene="lepB"
                     /locus_tag="PLM92_02880"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009082374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HPR24116.1"
                     /translation="MKTLIRDIAIVLVVILAITFFIKPIIVKKTSMEPTLMENNYLFI
                     SKQAYSFGEPERGDIIVFPHYTGGEKELYIKRVIGLPGETIETKDGKVYVDGEVLSEA
                     YIEDAYTTGQENGIKVPKGQLYVLGDNREVSIDSRYSEVGTVDIDEVTGKAILRVYPF
                     GEFGTL"
     gene            complement(804..1313)
                     /gene="lepB"
                     /locus_tag="PLM92_02885"
     CDS             complement(804..1313)
                     /gene="lepB"
                     /locus_tag="PLM92_02885"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009643116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HPR24117.1"
                     /translation="MKALIRDILIALAIAIAVSFLIRPVIVKETSMEPTLKENNYLIA
                     AKQAYRFGEIEYEDIIIFKSETDKEKLLIKRVIGLPGDVITISDGIVYRTGEILKEDY
                     IMGGESATTPGEIYNFEVPEGQVFVMGDNRQVSLDSRSDMLGTISKKSIKGKAVFRFY
                     PFSDAGKIH"
     gene            complement(1314..1853)
                     /gene="lepB"
                     /locus_tag="PLM92_02890"
     CDS             complement(1314..1853)
                     /gene="lepB"
                     /locus_tag="PLM92_02890"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HPR24118.1"
                     /translation="MSQGKYDSRSFKIRNRAYMYAIVAAIILFILVAPEMNEGKAMDP
                     TIKDGSFVVMTKESYSEKRGVPDLGQVVILEKTAAKELYEDNIIARVTGLPGDRIKIQ
                     AGTFYRNGKEYNVKNAKGSLGNDMDLKLKKNQVFLLCDNRDVVMDSRAKELGPVDMDM
                     IRGNAKWILWPLSDIGGIK"
     gene            complement(1834..2787)
                     /locus_tag="PLM92_02895"
     CDS             complement(1834..2787)
                     /locus_tag="PLM92_02895"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HPR24119.1"
                     /translation="MLKIEIGINDGGQRMDRFLKKYFGNASLSHIYKMIRKDIKIEGK
                     RQRPETILSPGDIICVYISEEEGQNLRKTKKAFAPVVRFDIAYEDEDMLIAEKPFGLL
                     IHGDASEKKNTLANQVLSYLKERGEYTPEKENTFRPAPVNRLDRNTTGLVIFGKNAEA
                     MKHLNRLIREREGIKKYYLTIVHGQIKEPLKLCGRLEKDTQKNTVRVLAEADQKGVCA
                     RTLIKPIKVGGDFTLTEAELITGRTHQIRAHLLSAGFPLIGDPKYGDSKINAEIKKRY
                     GLSTQLLHAYKLEIEGITVTAKLPDMFEKIKKELIDVPGKI"
     gene            2944..3117
                     /locus_tag="PLM92_02900"
     CDS             2944..3117
                     /locus_tag="PLM92_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPR24120.1"
                     /translation="MSKFKDFLYDKNDIMVALVILIVAAVIIFFRINAIMDYPSTQTD
                     QSGSAITEAVFKS"
     gene            3133..4071
                     /gene="fba"
                     /locus_tag="PLM92_02905"
     CDS             3133..4071
                     /gene="fba"
                     /locus_tag="PLM92_02905"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012064755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HPR24121.1"
                     /translation="MALVTSKYMFENALKSDHAIGAFNVNNMEIIQGIVDAAQTENSC
                     LILQVSAGARKYAKPAYLLKLVEAAIIDTGIDICLHLDHGEDFDICKKCVDDGFTSVM
                     IDGSGHPLKENMEITKKVVEYAHSKGVVVEAELGKLAGVEDNVSVDARQATFTDPDEA
                     AEFVQKTGVDSLAIAIGTSHGAYKFKGDPYLDFERLKKIHSLIPDTPLVLHGASTVLP
                     EFVAKCNEYGGDLPGTRGVPEEMILEAVKYGVCKVNIDTDLRLAMTAEIRKHLKEHPE
                     NFDPRKYLGPAREAIKGMVQHKIKNVLNSSGTGSDR"
     gene            complement(4206..5636)
                     /locus_tag="PLM92_02910"
     CDS             complement(4206..5636)
                     /locus_tag="PLM92_02910"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009643132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HPR24122.1"
                     /translation="MIEKLNMTMLTDFYEITMANGYFETGMADDIAYFDMFFREVPDE
                     GGFAIMAGVEQVASYLSNLKFTDEDIDFLRGKGIFDEKFLDYLKNFKFVCDVWTVPEG
                     TPVFPGEPIVTVRGPVIQAQFIETMILLLINHQTLIATKANRIVRAAKGRGVMEFGTR
                     RAHGVSAAIYGARAAYIGGCMGTACTVSERDYGIAALGTMAHSWVQMYPDEYTAFKKY
                     AEIYPENCVLLVDTYNALKSGVPSAIKVFKEMKPQKMGIRIDSGDVSYLTKKARKMLD
                     DAGLSECKIVVSNSLDEYIIEDVIRQGACIDSFGVGERLITAKSSPVFGGVYKLSALE
                     KDGKMIPKMKISENVGKITNPGFKTLYRFFDKESHHMRADLIMLDGEPEPSGDSFEIF
                     DPMAPWKRKTLTNFFVKNLRVQLFDKGKLVYDLPDIDKIRNYCALQISELWEEMLRFE
                     NPQTYYVDLSQNLYDLKQQIISEHSK"
     gene            complement(5620..6552)
                     /locus_tag="PLM92_02915"
     CDS             complement(5620..6552)
                     /locus_tag="PLM92_02915"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPR24123.1"
                     /translation="MFLTENHKARAHILMVLLVFLWGLEYVAAKWALESIATLTLVCL
                     KYVLAILLIGPIKLKMDGRSFFRKKDIPLFFACALFGEILYFFCEYEAMDYMPVSLIT
                     VILTFMPAASIVTEKLLFKRDPTIPMVVGIFICVFGIIMVIGADFDILFEGRFVGYLL
                     CFGAIFSWNTYNFMTAHLKEKYSAITLTFNQLVCTILLSLPFALSALPKPEDMTMPVV
                     GGILFLGFISAGVGFLVYVYALGVLGPTTMAVYSNFLPVTATLFGWILLGELIGGIQL
                     MGGVIVILAGYVVIKEKAKVDKKRLEVLSDDRET"
     gene            complement(6586..>7541)
                     /locus_tag="PLM92_02920"
     CDS             complement(6586..>7541)
                     /locus_tag="PLM92_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPR24124.1"
                     /translation="KSIDVKIPLSKFVCVTGVSGSGKSSLINEILYKGLSVKINRSKM
                     KAGIHEDITGIENIDKVIDINQSPIGRTPRSNPATYTGVFTHIRDLFAQLPESKIRGY
                     EKGRFSFNVKGGRCEACSGDGIIKIEMHFLPDVYVPCEVCKGRRYNRETLEVKYKGKN
                     IYEVLDMNVSEALEFFENLPSIKRQLETLEQVGLGYIKLGQPSTQLSGGEAQRIKLAS
                     ELSKRGTGKTLYILDEPTTGLHFADVDKLLNVLDRLADGGNTVVVIEHNLDVIKRADH
                     IIDLGPDGGFKGGEIIAEGTPEEIAGIKNSYTGQYLKKVIK"
BASE COUNT         1895 a         1777 c         1445 g         2424 t
ORIGIN      
        1 ctggttctta agggatcaac attgaatata ttcccctgtt catagggact tatgttcatt
       61 ccatgtatga atgcctcgcc gttttttatc ctggcataac tttcttttat acttactttg
      121 ccctgtctta tggcttttat ttctgttcct gtcagtacca gccccgcttc ataagtgtct
      181 tctataaaat aatcatgcct ggcttttctg ttttttgcta caactttttt ttctttcatt
      241 tatatacctt tatacctata aagtcccgaa ttcgccaaag ggatataccc ttaatatggc
      301 tttccccgta acttcatcga tatccaccgt ccccacttca gaatacctgc tgtcaatact
      361 cacttcccta ttgtctccca ggacatacag ctgtcccttc ggcactttta tgccgttttc
      421 ctgccccgtt gtataggcgt cctcgatata tgcttcactc agaacctctc cgtcaacata
      481 tactttcccg tcttttgttt ctatggtttc tccgggaagt cctattactc tttttatgta
      541 aagttctttt tcaccgccgg tataatgggg aaaaactatt atatctcctc tttcaggctc
      601 gccgaagctg taggcctgtt tgcttatgaa caaataattg ttctccataa gtgttggttc
      661 catggaggtt ttctttacta ttatgggttt aataaaaaat gttattgcca gtatcacaac
      721 cagtacgata gcaatatccc ttatgagagt tttcatgtga tttgtccttt cgatacaata
      781 cttgatgtct tttctttttt tggtcagtgg atctttcccg catcgctgaa gggatagaat
      841 ctgaaaacag ctttcccctt tatgcttttt ttgcttattg tgcccagcat gtcagatctg
      901 ctgtcaagac ttacctgtct gttgtctccc attacgaaga cctgtccttc cggaacttcg
      961 aagttatata tctctccggg agtggttgca ctttctcctc ccataatata atcttccttt
     1021 aatatctcac cggttctgta gactatgccg tctgaaatgg tgatcacatc cccgggcagt
     1081 ccaatgactc tttttatcag aagcttttct ttgtccgttt cggacttgaa aattattata
     1141 tcctcatatt ctatttcccc gaatctgtat gcctgtttag ccgctatgag ataattgttt
     1201 tctttgagtg tgggctccat ggaggtttct ttcactatta caggtcttat caaaaatgat
     1261 acggcaatgg ctattgctaa agctataagt atatctctga taagagcttt catttatttt
     1321 attcctccga tatcagaaag gggccaaagt atccatttcg catttcccct tatcatgtcc
     1381 atatccacgg gacccagttc ttttgctctg ctgtccatga caacatctcg gttgtcgcaa
     1441 agaaggaata cctgattttt tttgagcttc aaatccatat cgttccccag gctgcccttt
     1501 gcgttcttga cgttatattc tttaccgttt ctgtaaaatg tgcccgcctg tatctttatt
     1561 ctgtctccgg gcagaccggt aactcttgct attatgttat cctcgtaaag ctcttttgcg
     1621 gcggtctttt cgagtattac cacctgcccc agatcaggca ccccgcgttt ttctgaataa
     1681 ctctcttttg tcattaccac gaaactgccg tcttttattg ttggatccat cgccttgcct
     1741 tcattcatct ccggcgccac cagtataaaa agtattatcg ccgcaactat ggcatacata
     1801 taagctcggt tccttatttt gaaagatctg ctatcatatt ttccctggga catcgattaa
     1861 ctcctttttt attttttcaa acatatcggg cagttttgcc gtcactgtaa taccttcgat
     1921 ctcgagcttg tacgcatgaa gaagctgtgt tgaaagcccg tatctttttt tgatttcagc
     1981 atttatcttc gaatcaccgt atttgggatc acctataagc gggaatcccg cagataaaag
     2041 atgcgccctt atctgatggg tcctgccggt tatcagttcc gcttctgtga gagtgaaatc
     2101 tccgccgact tttatgggtt ttataagggt cctggcacaa acgccctttt gatctgcctc
     2161 ggccagcacc ctgaccgtgt ttttctgtgt gtccttttca agccttccgc acagctttaa
     2221 cggttctttt atctggccgt gcactatcgt gagataatac ttttttattc cttcacgttc
     2281 cctgataagt ctattaagat gcttcattgc ttctgcattt ttaccgaata tgacaagacc
     2341 ggttgtattt ctgtccagcc tgtttaccgg cgcgggcctg aaagtatttt ctttttccgg
     2401 ggtatattct ccccgctcct taagatatga aagcacctgg ttcgcaagag tgtttttttt
     2461 ctcgctcgca tccccgtgga tcaaaagccc gaaaggcttt tctgctatta gcatatcttc
     2521 atcctcatat gctatatcaa atctgacgac gggtgcaaat gcttttttgg tttttctcag
     2581 attttgccct tcttcttcag aaatatagac acaaattata tctcccgggg aaaggatagt
     2641 ttcaggtctc tgtcttttcc cttcgatctt gatgtccttt cgtatcattt tatatatatg
     2701 gctcaatgat gcattgccga aatacttttt taagaaacgg tccattctct gaccgccgtc
     2761 gtttataccg atttcaattt ttaacataca ttacattata caacaataaa gcttttgtaa
     2821 acagtcattg tgaagaaatt ttcataaaaa acggcggtct ttgcattttt ttgaaatttc
     2881 ccgtgacgca ctggtctgcc atggattttt gtgatattgt atatatgtga aaggagaatc
     2941 ggaatgagca aatttaaaga ttttctttat gataaaaatg atatcatggt ggctctggtg
     3001 atacttatcg tggctgccgt gatcatattc ttcaggataa atgccataat ggattatcct
     3061 tccactcaaa ctgatcagag cggctcggca ataacagagg ctgtttttaa atcataataa
     3121 aaggaggtta taatggcttt agttacaagt aaatacatgt ttgaaaacgc ccttaaaagc
     3181 gaccatgcca taggtgcttt caatgtaaac aacatggaga tcattcaggg tatcgttgat
     3241 gcggcgcaga ccgaaaattc atgtctcatc cttcaggttt cggcaggggc cagaaaatat
     3301 gccaaaccgg catatcttct gaagcttgtg gaagcagcca taatcgatac cggcatcgac
     3361 atctgcctcc atctggacca cggagaggat tttgatatct gcaaaaaatg cgtagatgac
     3421 ggttttactt ccgtcatgat cgacggctcc ggacatcctc ttaaggaaaa catggaaatc
     3481 acaaaaaaag ttgtggaata tgcccacagt aaaggtgttg tggtggaagc cgaactgggc
     3541 aaacttgcag gagttgagga caatgtaagc gtggacgccc ggcaagctac attcacagac
     3601 ccggatgaag ctgcggaatt cgttcaaaaa accggtgttg attctctggc catagccatt
     3661 ggaacaagtc atggagcata taaattcaag ggggatcctt atcttgattt tgaaagactg
     3721 aaaaagatcc actcccttat ccccgatacc cctctggtac tgcatggagc atcaacagtg
     3781 ctgccggaat tcgttgccaa atgcaacgaa tacggcgggg atcttcccgg aacaaggggt
     3841 gtacccgaag aaatgatact ggaggcagtt aaatacgggg tgtgcaaagt caatatagat
     3901 acagacctta gactggcaat gacagcagag ataaggaaac atctaaagga gcatcccgaa
     3961 aatttcgacc cgcgaaagta tctgggtcct gcaagggaag ccataaaggg catggtgcaa
     4021 cacaagatca agaatgttct caattcatca gggacaggtt cggaccggta atacatcgat
     4081 tcagaggtat tctctaactc aagaatattt atggatctga aaacaagaca aataaaatga
     4141 ccgcgcatgc ggtcattttt tggtaatccc aaatatatct cttcctgctg tttttttcct
     4201 gcagtctact tgctatgctc acttattatc tgctgtttaa ggtcatacag gttttgtgaa
     4261 aggtccacat aataggtctg ggggttttcg aatctgagca tttcctccca gagctcgctt
     4321 atctgtaatg cacagtagtt tcttatctta tctatgtcag gcagatcgta tacgagtttt
     4381 cctttgtcaa aaagctgcac cctgaggttt ttgacaaaga aattcgtaag tgtctttctt
     4441 ttccaggggg ccataggatc gaatatctca aaagaatctc cggaaggttc cggctcgccg
     4501 tccagcatta taaggtcagc tctcatatgg tgagattcct tatcaaagaa tctgtaaagt
     4561 gtcttgaatc ccgggttggt tattttgccc acattttcgc ttatcttcat cttcggtatc
     4621 atttttccat ccttttcgag ggcggaaagc ttataaacac ctccgaaaac aggagatgat
     4681 tttgccgtta tgagcctttc tcccacaccg aaggaatcta tgcatgctcc ctgtcttatt
     4741 acatcctcta ttatatattc atccagggag tttgatacaa ctatcttaca ttccgaaagc
     4801 cccgcatcat ccagcatttt tcttgctttt ttggtaaggt aactgacgtc tccgctgtct
     4861 atcctaatgc ccattttctg cggtttcatc tctttgaaaa ccttaattgc agacggcact
     4921 ccgctcttaa gggcattata tgtatcaacc agaagaacgc agttttcagg atatatctct
     4981 gcatattttt tgaatgctgt atattcgtcc ggatacatct ggacccagct gtgagccatc
     5041 gtgcccagag ccgcgatccc gtaatctctt tcggatacgg tacaggccgt ccccatacat
     5101 ccgcctatat atgccgccct tgctccgtat atggcagcgg aaacaccatg ggctcttctg
     5161 gtcccgaatt ccataacgcc ccgtcctttg gcggcacgaa ctatcctgtt ggctttggtg
     5221 gctatcaaag tctgatgatt tataagcaaa agtatcatgg tctctataaa ctgagcctgt
     5281 atgaccggac ccctgactgt tactatgggc tctcccggaa aaaccggtgt gccttccgga
     5341 actgtccata cgtcacaaac gaatttgaag tttttcagat aatcaaggaa tttctcgtca
     5401 aaaatgcctt tgcctctcaa aaaatctatg tcctcatcgg tgaatttcag attactgaga
     5461 tatgatgcaa cctgctctac tcccgccata atggcaaatc cgccttcatc gggcacttct
     5521 ctgaaaaaca tatcaaaata ggctatatca tctgccatgc ctgtctcaaa gtatccgttg
     5581 gccatggtga tctcatagaa atctgtcagc atggtcatat taagtttctc gatcatctga
     5641 aagtacctcc agtctttttt tgtcgacttt tgctttttct tttataacta catatcctgc
     5701 caagataact attactcctc ccataagctg tataccgcct atcaactcac ccagcagtat
     5761 ccatccgaac aatgtcgccg tcaccggcag aaaattggaa tacaccgcca ttgttgtggg
     5821 tcccagaacg cccagagcgt atacataaac cagaaacccc actcctgcac tgataaatcc
     5881 gaggaaaagt atccctccca caacaggcat tgtcatgtct tccggttttg gcaaggccga
     5941 aagtgcaaag ggcagcgaaa gcagtattgt gcataccagc tggttgaatg tcagcgttat
     6001 tgccgaatac ttttctttca ggtgagccgt catgaaatta tatgtattcc atgagaaaat
     6061 cgccccaaag caaagtagat acccgacaaa tcttccttca aacagtatat caaaatccgc
     6121 accaattacc attattattc cgaaaacaca tatgaatatt cctaccacca tgggaatggt
     6181 cggatctctt ttgaaaagga gtttttcggt tactattgat gcggccggca taaatgtcag
     6241 tatcacggtt atcagcgata ccggcatata gtccatggct tcatattcac aaaaaaaata
     6301 aagtatctct ccgaacaggg cacacgcaaa aaaaagcggt atgtcttttt tcctgaaaaa
     6361 gctccttccg tccattttca gttttatagg tcctattaac agtattgcca gcacatattt
     6421 caagcatacc agcgtcagcg tggcaattga ctcaagtgcc cattttgcgg ccacatactc
     6481 aagcccccat aaaaatacaa gaaggaccat caaaatatgg gcgcgggctt tatggttttc
     6541 tgtcaggaac aaaatttcac catctctcct aatgctttac tttctctatt ttatcacctt
     6601 tttgagatat tgtcctgtat aagaattttt tatccctgca atttcttcgg gagttccctc
     6661 tgctattatc tcacccccct tgaatcctcc gtcgggtccc agatctatta tgtgatctgc
     6721 tctctttatg acatcaagat tatgttctat gactaccaca gtgttgccgc cgtctgccag
     6781 tctgtccaga acgttaagaa gcttatccac atcggcaaaa tgaagaccgg tcgtgggctc
     6841 atcaaggata taaagggtct tgcccgtacc tctttttgaa agttctgatg caagttttat
     6901 cctttgggcc tcacctccgg aaagctgtgt ggatggctgt cccagtttta tgtatccaag
     6961 acccacctgt tccagagttt caagttgtct ttttatggaa gggagatttt caaaaaactc
     7021 caatgcctct gaaacgttca tgtccaggac ttcgtatata ttctttccct tgtatttgac
     7081 ttccagggtc tcccggttat atctcctgcc tttgcatact tcacagggca cataaacatc
     7141 cggcaggaaa tgcatctcaa tttttattat gccgtcgccg ctgcaggctt cacatcttcc
     7201 gcctttcacg ttaaagctga atctgccctt ttcatatcct cttatttttg attcgggcag
     7261 ctgtgcgaaa agatctctga tatgggtgaa cactcccgta tatgtggcag gattggatct
     7321 tggggtcctg cctatgggtg actggtttat gtctatgact ttgtcaatat tttcgatacc
     7381 ggttatatct tcatgtatgc ccgctttcat cttcgagcgg ttgattttga cagacagccc
     7441 tttataaagt atttcattga taagactgct tttaccggaa cccgatacac cggtgacaca
     7501 aacgaatttg cttaggggga tctttacatc tatgcttttg a
//
LOCUS       DAONHK010000048         7325 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_7856_length_7325_cov_4.287345, whole genome shotgun sequence.
ACCESSION   DAONHK010000048 DAONHK010000000
VERSION     DAONHK010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 7325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7325
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7856_length_7325_cov_4.287345"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(112..690)
                     /locus_tag="PLM92_02925"
     CDS             complement(112..690)
                     /locus_tag="PLM92_02925"
                     /inference="COORDINATES: protein motif:HMM:NF016356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-thiouracil desulfurase family protein"
                     /protein_id="HPR24125.1"
                     /translation="MNRHEDARSKKVIFVASCLMNTNNKVKGLARYPGMCKEVFDTLY
                     ENDLGIQQMHCPETLYLGIQRWWQTKNLYDSHGFRSFCRKLATDVVTYMEEYEREGYK
                     TVAVLGCDGSPTCGVSITCWDKSWGGSPVDMDFNKAITEGSGVYMEELKKEIETRGFD
                     IPPFYGLALDDESADMDKIISDFREFIKGISG"
     gene            complement(724..888)
                     /locus_tag="PLM92_02930"
     CDS             complement(724..888)
                     /locus_tag="PLM92_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPR24126.1"
                     /translation="MLPEAGYKEVSAEYGKVYFDSRITEISDIVELGFKDYPFASWIT
                     AEGVEPMPKG"
     gene            complement(1037..1576)
                     /locus_tag="PLM92_02935"
     CDS             complement(1037..1576)
                     /locus_tag="PLM92_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008711510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HPR24127.1"
                     /translation="MSVEKAYKKLTKEQLWQKCLDFHGHDCGGLTVGFKAALYAAELL
                     DMDFSEDEEVVCIAENDACGVDSIQVILGCTAGKGNLIFKIQGKMAFNFYDRKTGKSL
                     RLILKKRIKDKKKIPDMGGDDLFDVTPVHFPFPQKAELYDSHRCSLCGEETAEPFLLK
                     KDGRNICMHCAEEDGRGGV"
     gene            complement(1657..2910)
                     /gene="uvsE"
                     /locus_tag="PLM92_02940"
     CDS             complement(1657..2910)
                     /gene="uvsE"
                     /locus_tag="PLM92_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012060638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009650 - UV protection [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UV DNA damage repair endonuclease UvsE"
                     /protein_id="HPR24128.1"
                     /translation="MRIGYACLAVAVPGSEMKSCTLKNADHERLLELIAHNLDSLEKL
                     IDYNIKNGIKLFRISSDIIPFGSGIAADIPWEHIFAEKFFKTGQKIKDSGMRVSMHPG
                     QYTVINSPKSAVVSAAEKDLEYHAKILDALGINTEHKIVLHMGGTYGDKEEAKKRFVS
                     RCRNLKPALTARLVIENDDRSFNIGDVLESAEKLKLPVIYDNLHNAVNRADDLTSDVQ
                     WIKKCKSTWREKDGPQKIHYSQQNKDKRPGAHSETIKTEEFIGYYQDLPGKDIDIMLE
                     VKDKNMSAVKCINCVSKRGIKELEKEWGRYKYLVLEHSPAGYQAVRNMLKDKKSYPAV
                     PMYLTIENSMDKKKIKGDAVNAAQHVWGYFKNKASENEKNRFEKILQKYLLGGSEIKS
                     IKKYLLTLSKKYCEDYLLEGYYLYK"
     gene            complement(2927..3967)
                     /gene="hypE"
                     /locus_tag="PLM92_02945"
     CDS             complement(2927..3967)
                     /gene="hypE"
                     /locus_tag="PLM92_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HPR24129.1"
                     /translation="MRITMAHGGGGKDSADLMKNIFGKYFGNEILDKMEDAAVIDPFL
                     LCDMENKKMGKLACSTDSFVVTPLEFPGGDIGKLCVCGTLNDLLMMGAKPLYITCGFI
                     MEEGLETDLLERIVSSMAETAKEAGIKIIAGDTKVIEGSGGLYINTTGMGHVPNGRDI
                     SAANCRKGDKILLSGNLGEHHACILSARMEIENGIRSDCAFLSDVTETLLDAGINVRT
                     MRDVTRGGLATVLNEIAESSCCEMVIREKDIPVSPDVKGLTDILGLDPMYMANEGKMI
                     AVIPEEEAETALDSVKKTVHGKDAVIIGEVSEILKGSATSDTDARVKLITHLGGTRVL
                     DMLYGEGLPRIC"
     gene            complement(3982..5025)
                     /gene="hypD"
                     /locus_tag="PLM92_02950"
     CDS             complement(3982..5025)
                     /gene="hypD"
                     /locus_tag="PLM92_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012199766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HPR24130.1"
                     /translation="METATVIENLKNYDGPELKLMEVCGTHTASIFKSGIRSMISPAI
                     KLISGPGCPVCVTPAAYIDKCAEYAMKKGHILLTFGDMMKVPGTDGSLSDLKGSGASA
                     ELMYSPFEAVERAAAHPETMFVVAAVGFETTAPAYALAIEDAKKRGIKNLKFITALKT
                     IFPALSWICENQPDIDGFICPGHVSVIIGIRPYERLAAKYKKPFVIAGFEAEHILAVI
                     YDIVNQIKKGEARAKNLYENAVREQGNIKALDIIKKYFEEGPAMWRGLGTIERSGLYL
                     KKDSPFDAGSRGLDADIKLPGECRCGDVIVGRINPDECPMFAKGCRPDAPYGPCMVSG
                     EGACGIWYRNRKL"
     gene            complement(5025..5240)
                     /locus_tag="PLM92_02955"
     CDS             complement(5025..5240)
                     /locus_tag="PLM92_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004602912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HPR24131.1"
                     /translation="MCIAIPGKVISLENNKAKVDFSGNTVTVNTGLVDPRPGQYVLVH
                     AGCAIEVMEKEKAEELLEIFADLEDFR"
     gene            complement(5240..5725)
                     /locus_tag="PLM92_02960"
     CDS             complement(5240..5725)
                     /locus_tag="PLM92_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006572665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HPR24132.1"
                     /translation="MAIEKVRQYFREFGLEEEIKEFDVSSATVELAAKAVGVIPARIC
                     KTLSFSTPDGGCILIQTAGDTKIQNGKYKSKFGFKAKMLEPDQVGRFTGHEIGGVCAF
                     AVEDPEVRIYTDISMKRFDTVFPACGSSNSAIELTCDDLFDHSKALEWIDVCKIVQEE
                     E"
     gene            complement(5707..>7325)
                     /locus_tag="PLM92_02965"
     CDS             complement(5707..>7325)
                     /locus_tag="PLM92_02965"
                     /inference="COORDINATES: protein motif:HMM:NF013468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HPR24133.1"
                     /translation="EEKLISSSARPIVLLEKRSEGMPQKGGVRGKWLPEFEKSRFIGS
                     FLPSFGGQYTLLDFVGGPLISTSANLSDMPMIKSDEEMTEMTKREPRIAAVFFNKRDI
                     RVSVDDSVVRVIDNNPQMIRRSKGYAPVPLYLGTNNSNEKSKKTDGKKADRKITDGKD
                     PSDMQIFAAGGHLKNSFALSKGPFVYVSQYFGDLDSRENQVIYEDSLTHMKELFRISP
                     GAVVCDMHPSYYTTGYAEKYAKDNKIPLLMVQHHHAHIASVMAEHDLDGPVIGVGFDG
                     TGYGMDGAIWGGEFLLCRESDFERKAHLKYIKMIGGDSSMKEGWKSALSYIRAAEEGP
                     ENGTGLDGPRQIVPDIKEIVEYARENSFTERINREVTAEGMAAGGLGAADLVNTALAR
                     DINTINSSSIGRLFDAVASMLDICDNNSYEGRCAIMLEDAAARALMNKAEDRADKLAL
                     KFHFMIAEMILEQCRIIREEEGTDGKVALSGGVFQNRILMDKTLSLLRDNGFKPYYNI
                     SVSPNDGGIALGQTYITTQMIRSEKWPSKK"
BASE COUNT         1720 a         1851 c         1292 g         2462 t
ORIGIN      
        1 tatttctaaa ttctatatca gtttttacag tttacacaga acttgaaact ctcccatata
       61 atcaaaaacg ggcacacata tgatgtgccc gttttttaaa atgtattatt cttaaccgct
      121 tattcccttt ataaattctc tgaaatcaga tattatctta tccatatccg ccgattcatc
      181 atcaagggca agaccgtaaa acggcggaat atcgaatccc ctggtctcta tctctttttt
      241 gagttcctcc atgtaaactc cggatccctc tgttattgcc ttattgaaat ccatatccac
      301 cggacttcct ccccagcttt tgtcccagca tgttatggaa acaccgcagg tgggggaacc
      361 gtcacatccc aggacggcta ctgttttata tccttccctt tcgtattcct ccatataagt
      421 aaccacatct gtagcaagtt ttctgcaaaa gctgcggaat ccatgactgt cataaaggtt
      481 ttttgtctgc caccatcttt gtatgccaag ataaagagtc tcaggacaat gcatctgctg
      541 tatgcccaga tcgttttcat aaagagtgtc aaaaacttct ttgcacatac ccggatagcg
      601 ggcaagtccc ttgactttgt tattagtgtt cataagacaa ctagctacaa aaatcacttt
      661 tttactgcgt gcgtcttcgt gtctattcat aattacacct ccattttttg taataaccgg
      721 ttttcagccc tttggcatgg gttctactcc ttctgccgtt atccagcttg cgaaaggata
      781 gtctttgaac cccagttcaa ctatatctga tatttctgtt attctgctgt caaaatagac
      841 tttgccgtac tctgcactga cctctttgta accggcttcc ggcagcaatg ttttgtcttc
      901 tttttccgca aaccttgcgg atacgctgtc atggctactt gctcagcttt ggcatctgtt
      961 catatcgaat actattccat cgatactgtt ttctttcata taacttataa gattctcttc
     1021 cattcttatc ttcatttcat actcctcctc tgccatcttc ttctgcacaa tgcatacaaa
     1081 tatttctgcc gtcttttttc agtaaaaaag gctctgccgt ctcttcgccg cataaagaac
     1141 acctatgtga atcataaagt tctgcttttt ggggaaaagg gaaatgcacc ggtgtgacat
     1201 cgaaaagatc gtctcccccc atatccggaa tctttttttt gtcttttata cgctttttca
     1261 agatcagtct caatgatttt cctgttttgc ggtcatagaa attaaaggcc atttttccct
     1321 gtattttgaa tatcagattt ccctttcccg cagtgcatcc caggatcacc tgaatggaat
     1381 caaccccaca ggcatcgttt tcggcaatgc ataccacctc ttcgtcttcg gaaaaatcca
     1441 tatccagcag ctccgccgca taaagtgccg ccttgaaccc tactgtcaga cctccacaat
     1501 cgtgtccatg aaaatccaga cacttttgcc agagttgttc ttttgtaagc tttttataag
     1561 ccttttcaac gctcatcctg ctgccactcc ctcttgcttt ctattttttg gttcttttag
     1621 tttgttttgc aaatatatta taatatattt gcaggattat ttatacagat aatacccttc
     1681 aagcagataa tcctcacagt atttttttga aagggtcagc aaatattttt ttatgctttt
     1741 tatctccgag cctcccaaaa gatatttttg cagtatcttt tcaaaacggt ttttctcgtt
     1801 ttcggaagct ttgtttttga aatatcccca tacatgttgt gccgcattta ctgcatcgcc
     1861 ctttattttc tttttatcca tactgttctc gatggtcaga tacatgggaa cagccggata
     1921 tgattttttg tccttgagca tatttcttac tgcctgataa cccgccggtg aatgttccag
     1981 aacaaggtat ttatacctcc cccactcttt ttcaagctcc tttatccctc tttttgaaac
     2041 acaattgata catttcactg cggacatgtt tttatctttt acttcaagca tgatatctat
     2101 atcttttccg ggcagatcct ggtaatagcc aatgaattcc tccgttttga tggtttccga
     2161 atgtgccccg ggtcttttgt ctttgttctg ctgagaatag tgtattttct gagggccgtc
     2221 tttttctctc catgtacttt tgcatttttt gatccactgg acatcggatg tgagatcatc
     2281 ggctctgttt acggcattat gaagattgtc atatatgaca ggaagtttca gtttctctgc
     2341 cgattccagt acatcgccga tattgaatga ccggtcatca ttttcaatca ccagtcttgc
     2401 tgtcaaagcc ggcttcagat tcctgcagcg ggacacaaat cttttttttg cttcttcttt
     2461 atccccatag gtgccgccca tatggagtac tattttatgt tctgtgttta taccaagggc
     2521 atcgagtatt tttgcatggt attccaggtc tttttctgcc gcagaaacaa cggcgctttt
     2581 gggagaatta atgactgtat actgacccgg atgcatggaa acacgcattc cggaatcctt
     2641 tattttttgt cctgtcttaa aaaatttttc tgcaaagata tgttcccagg gaatgtctgc
     2701 tgctataccg gaaccgaatg gtataatatc tgaagatata cgaaaaagct ttattccgtt
     2761 ttttatgttg taatctataa gtttttcaag agaatcaaga ttgtgtgcta tcaactcaag
     2821 aagtctttca tggtcagcat tctttagtgt acagcttttc atttcactgc cgggcaccgc
     2881 aactgccaga caggcatatc ctatcctcaa aattcaacct ttccctttag catatcctgg
     2941 ggagcccttc cccgtaaagc atgtcaagta cccttgtacc tcccagatga gttataagct
     3001 taactctggc atcagtatca gatgtcgcgc ttccctttag aatttctgac acttcgccta
     3061 ttattacagc gtcttttcca tgtacagtct ttttcactga atcaagagcc gtttccgctt
     3121 cttcttccgg gataacagct atcattttcc cctcatttgc catgtacatg gggtccagcc
     3181 ccagtatatc tgtaagcccc ttgacatcgg gacttaccgg tatgtctttt tctcttatga
     3241 ccatttcaca gcaggaagac tctgcgatct catttagtac ggtggccagt cctcccctgg
     3301 tcacatctct catggttctt acatttattc ctgcatccaa aagggtttct gtcacatccg
     3361 aaagaaaagc acagtcactt cttataccgt tttctatttc catcctggca cttaatatac
     3421 acgcatgatg ttctcccagg ttaccggaaa gcaggatctt atcccctttt ctgcagtttg
     3481 ccgcacttat gtcccggcca ttgggaacat gccccattcc tgttgtgttt atataaagtc
     3541 cgccggatcc ctcaattact tttgtgtctc cggctatgat ctttatgccc gcctcttttg
     3601 cagtctctgc catggatgat actattcttt caagcagatc tgtttcaagt ccctcttcca
     3661 ttatgaaacc gcatgttata taaagaggct ttgctcccat catcagaaga tcgttcaatg
     3721 tgccgcacac acagagtttt ccgatatctc cccccggaaa ctccagggga gtcaccacga
     3781 aactgtcggt actgcaggcg agctttccca tttttttgtt ttccatatca cataataaaa
     3841 acggatctat cacagccgca tcttccatct tatcaagtat ttcatttcca aaatattttc
     3901 cgaatatgtt tttcatcagg tcggcggaat cttttccacc tccgccatgg gccattgtta
     3961 ttctcattgg gtcttttcct ttcataactt tctgtttctg taccatatgc cgcatgcacc
     4021 ttctcctgaa accatacacg ggccataagg ggcatccggt ctgcatcctt tggcaaacat
     4081 ggggcattca tcggggttta tcctgcctac gataacgtct ccgcaacggc attcaccggg
     4141 cagttttata tccgcatcca ggcctctgct tcccgcatcg aaaggtgaat cttttttcag
     4201 ataaagcccc gacctttcta tggtgcccag gcctctccac atggccggtc cctcctcaaa
     4261 atattttttt attatatcaa gagcttttat gttaccctgt tctctcaccg cattctcata
     4321 aagatttttt gctcttgctt cacccttttt tatctgattt acaatatcat aaatcacagc
     4381 aagtatatgc tccgcctcaa atcccgcaat aacgaacggc tttttatatt ttgcagccag
     4441 cctttcgtaa ggcctgatgc cgattattac acttacatgt ccgggacata tgaaaccgtc
     4501 tatatcgggc tgattttcgc atatccagga aagggcaggg aaaatggttt taagtgccgt
     4561 aatgaatttc agatttttga tgccccgttt ttttgcatct tcgatggcaa gagcatatgc
     4621 cggtgcagta gtttcaaacc cgacggctgc cacaacaaac atggtttcgg gatgagccgc
     4681 agctctttcc accgcctcaa agggtgagta catcagttct gcggaagctc cgcttccttt
     4741 aagatctgaa aggcttccgt cagttcccgg aactttcatc atatctccga aggtcaaaag
     4801 gatatgccct tttttcatgg catattctgc acatttatct atatatgcgg caggagtcac
     4861 gcatacaggg caaccgggcc ccgatataag ttttattgca ggagatatca tgcttcttat
     4921 accgcttttg aaaatagatg ctgtatgagt accgcatact tccataagct taagctccgg
     4981 tccgtcatag ttttttagat tttctattac tgtagccgtc tccattatct gaaatcctcc
     5041 agatccgcaa atatttccag cagttcttct gctttttctt tttccatcac ttcaatggca
     5101 catccggcgt ggacaagaac gtactgtccc ggtctgggat ccaccagacc tgtattcaca
     5161 gtaaccgtat tcccgctgaa atcaactttt gctttattat tttcgaggga aatgactttc
     5221 cccggtatcg caatacacac tattcttcct cctgtactat tttgcataca tctatccatt
     5281 caagggcttt tgaatgatca aaaaggtcat cacatgtcaa ttctatggcg ctgttgctgc
     5341 tgccgcaggc gggaaaaaca gtatcaaacc ttttcattga tatatcagta taaattctca
     5401 cctcgggatc ctctacggca aatgcgcaaa cccctcctat ttcgtgacct gtaaatcgcc
     5461 cgacctgatc gggttcaagc atttttgcct tgaatccgaa ttttgactta tatttcccgt
     5521 tttgtatttt tgtgtctccg gctgtctgta taagtatgca cccgccgtca ggagtgctga
     5581 atgaaagtgt tttgcatatc ctggcgggga tgactcctac ggcttttgcc gccagttcaa
     5641 ctgtggcgct ggaaacatca aattccttta tttcttcttc cagtccaaac tccctgaaat
     5701 actgtcttac tttttcgatg gccatttttc gctccttatc atctgtgttg taatatacgt
     5761 ctgtcccaga gctattcccc cgtcattggg acttactgat atattataat atggtttgaa
     5821 tccgttgtca cgaagcagac tcaaagtttt atccatgagt atcctgttct ggaaaacacc
     5881 tcccgaaaga gccacttttc cgtctgtgcc ttcttcttcc ctgattatac ggcattgttc
     5941 aagtatcatt tctgctatca tgaaatgaaa tttcaaagcc agtttatcgg ctctgtcttc
     6001 tgctttgttc ataagcgctc tcgccgcagc atcttcaagc ataattgcac agcgtccttc
     6061 ataactgtta ttgtcacata tatcaagcat ggatgccact gcatcaaaaa gcctcccaat
     6121 gctggaactg tttattgtat ttatatcccg ggcaagggct gtattcacca gatcggcagc
     6181 gccaagacct ccggctgcca tgccttctgc ggtaacctcc ctgtttatcc tttccgtaaa
     6241 agaattttct cttgcatatt ccactatctc ttttatgtca ggaactatct gccttggccc
     6301 atcaagtcct gtcccgtttt caggtccttc ttccgctgct cttatataac tcaatgcgga
     6361 tttccatcct tccttcatgg aagaatctcc gcctatcatc tttatatatt tcaaatgggc
     6421 ttttctttca aaatcacttt cccggcaaag cagaaactct cctccccaga tggctccgtc
     6481 catgccgtag cctgtaccat caaaaccgac tcctatcaca ggcccgtcaa ggtcgtgctc
     6541 ggccattaca gaggcaatat gggcatgatg atgctgcacc attaagagag gtattttgtt
     6601 atcctttgca tacttttctg catatcccgt agtataatat gaaggatgca tatcacatac
     6661 aacggctccg ggacttatcc tgaaaagctc tttcatatga gtcagactgt cttcatagat
     6721 cacctggttt tccctgctgt ccagatctcc aaagtactga ctcacataca caaagggtcc
     6781 ctttgaaagg gcaaaggaat ttttcaaatg cccgccggcg gcaaaaatct gcatatcaga
     6841 tggatctttt ccgtctgtta tttttctatc tgctttttta ccgtccgttt ttttgctttt
     6901 ttcgttactg ttatttgtgc ccagataaag ggggacaggt gcatatcctt tggagcgtct
     6961 tatcatctgc gggttattgt ctattaccct tacgaccgaa tcgtcaacgc tcacccttat
     7021 atctctttta ttaaaaaaca ctgccgcgat ccgaggctct ctttttgtca tttctgtcat
     7081 ttcttcgtcg cttttgatca taggcatatc agaaagattg gcactggtgg atatcagtgg
     7141 cccgcctacg aagtccagca atgtatactg tcccccgaaa ctgggcagaa aacttcctat
     7201 gaatctgctt ttttcaaatt ccggtagcca cttgcctctt acaccgccct tttgaggcat
     7261 tccttcgctt ctcttttcca gaagcactat aggtcttgca gacgaagaaa taagtttttc
     7321 ttccg
//
LOCUS       DAONHK010000049         7032 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_8272_length_7032_cov_4.805934, whole genome shotgun sequence.
ACCESSION   DAONHK010000049 DAONHK010000000
VERSION     DAONHK010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 7032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7032
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8272_length_7032_cov_4.805934"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /locus_tag="PLM92_02970"
     CDS             <1..523
                     /locus_tag="PLM92_02970"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPR24134.1"
                     /translation="GAEVFCLGLIDGDLSYNTILDVIYNKGITSIVGIPDQVHGLMKH
                     TASLKMKTVLLSGEYVSPIVASDISKIWGCRVFEHYGMREMGLGCAVSCHVLKGYHVR
                     ETDIFIEIVDTESRKPVNDGTWGEIVFTTLTRKGMPFIRYRTGDEGRWLKDPCPCGSV
                     LKRLDRVRKRRIK"
     gene            520..1350
                     /locus_tag="PLM92_02975"
     CDS             520..1350
                     /locus_tag="PLM92_02975"
                     /inference="COORDINATES: protein motif:HMM:NF024869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC/CoxI family protein"
                     /protein_id="HPR24135.1"
                     /translation="MSNKVVYFARDLLEKGEDFVIARIVETKGSTPRKSGALMLMRSD
                     GTFSGTVGGGRIEAETEKLCREAYKTKKKSRIYHFELNSKEKNSLDMGCGGNADIIIE
                     YIDAGDPGSFLEDYKISDIAYIFGAGHVGLALEPVLRHINFSTVVIDDREDYANRERF
                     PEATDVRTIKSFEHSFDDITCDENSYIIIVTRGHMGDYEVLRDALKQKPAYIGMMGSR
                     EKNSTIRDMLKEKDGFTDAEIDQVYAPIGENIYAETPEEIAVSIAAELIRVRTGNGTR
                     "
     gene            1431..2645
                     /locus_tag="PLM92_02980"
     CDS             1431..2645
                     /locus_tag="PLM92_02980"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HPR24136.1"
                     /translation="MINKRKVGAVILAAGYSSRMEKFKPLMLIGEKTAAERIIDSARS
                     AFVDDIVLVTGYEREKLIPVIEKSGIREAYNPKYDKGMFTSIKAGIEALGDELDGFFI
                     VPVDCPLVMKETFEMLVSESPEFFTVPTYMGKKGHPLYVPMIYKEEIIKSESKTGLKA
                     IVDIYGDKLILKDSASESVVLDMDTNADYRDLLEYYSEGCREKDLGELAAGRRFIFVR
                     HGKTEQHKEKIFLGQTDIPMSQKGTEQVEIAAFEISRLKLMTGHIYSSDLKRASETAE
                     IICGMTVISAVIYEKDFREMSLGEWDGKFISSIKERYPEEYEKRGKDIFRYKFDHAAE
                     NFFDLQYRVIKRLKKILEQDHRKDIVITAHSGVIRVIENNLKGLDVSDEWEPLENGAL
                     RVIEMPERKSPL"
     gene            complement(2651..3454)
                     /locus_tag="PLM92_02985"
     CDS             complement(2651..3454)
                     /locus_tag="PLM92_02985"
                     /inference="COORDINATES: protein motif:HMM:NF024197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HPR24137.1"
                     /translation="MKASEKRLLRRQLAMAEKKEKKYLSYRADSKMNIDVFSGIRQKI
                     PEKALSALETAFEKGFFFIFEKGTGLIEKTGNFDQIKAQLALNRYELQMGINKETLKI
                     FDVAAGNKSIGNKSLSTVEGAALGVFGIGLPDIPVFLGVIFKSIYEIAISYGFDYDSD
                     AERAYILSIIKIAASKEAGKIIASHECDLLGEKIDCGEIPQDVLTEEDIRDTSNMLAT
                     SLLVAKFIQGITIVGSIGAVFNYSWVSKISKIAKIKYKKRFLAGLLRNA"
     gene            complement(3456..4220)
                     /locus_tag="PLM92_02990"
     CDS             complement(3456..4220)
                     /locus_tag="PLM92_02990"
                     /inference="COORDINATES: protein motif:HMM:NF015949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF364 domain-containing protein"
                     /protein_id="HPR24138.1"
                     /translation="MWKAYDTLIGSLPDDIEVTDVHSGPVWTIVQAGELCGIAVTVNE
                     QNAPLPERKRFIGQSLKDVASLCRSWDFPLASIGTAALNAYINSPEQIRKTFGTYDEN
                     SKKNTGIMIHTGNTFDDYSRAVSGKKVAVIGHFTALERVLKSAENLIVLERKPDKGDL
                     PDTACEYVLPYQDFVFITGSAFINKTLFRLLEISRNARTVVLGPSTPMAPVLFGYGAD
                     ELGGLITENIPPEKFSEIGKDTFRLSSIGERIRLVK"
     gene            complement(4196..4783)
                     /locus_tag="PLM92_02995"
     CDS             complement(4196..4783)
                     /locus_tag="PLM92_02995"
                     /inference="COORDINATES: protein motif:HMM:NF015239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017111 - ribonucleoside triphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphatase"
                     /protein_id="HPR24139.1"
                     /translation="MKKHLFLEGQIRTGKSTLLRDYLRPYRHNIGGFCSQRLIDENGE
                     AFAYRITDAEILDVEASYSPDLEHIFAVRSDKGAQKDMEVFEKCGVKLLKEARKKKLI
                     LLDEIGGSELLVPAFRNELYKVLGGNISCIGVIKLNEKAKFMSREAGYPGIVADLNAQ
                     LRKDLIDRFDSRIIPFDENSKRYIEEFLCGKLMTL"
     gene            4859..5566
                     /locus_tag="PLM92_03000"
     CDS             4859..5566
                     /locus_tag="PLM92_03000"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HPR24140.1"
                     /translation="MLSYITERKTGKDSGNGQMEPKEKIEIISRSPLFEGLSKEECLT
                     TYEDIKPQTRKYSRNEIIVGEGERMNFIGIVVSGKVRGEKFHMEGDVHLVYMYETEEL
                     FGLDTAATRTRIAPVTFLADKNSSVLTVNMDRIMGSSMQGKLVLNMVRMLADENIRKL
                     YKIEAISRRGLRDRIMTYLNIRQKKLGRNEFRIMMTQEQFAQYLCVNRSALTWELAKM
                     KKEGIIDFSKDSFILKE"
     gene            5770..7026
                     /locus_tag="PLM92_03005"
     CDS             5770..7026
                     /locus_tag="PLM92_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPR24141.1"
                     /translation="MEFFDMVQRKRASKVIAIFLAICLIFTMGMYVTTDIAKGVEDEP
                     RARNGEDWTSAGAPDLSALPENLFLIEVTGPAGIKTVQVKKSNILTLRFYQGAYKWGS
                     LDKQQATGTGISLGDVFKAEGIDTAGITKFEEFATDNTPSGDLVSDINGKYNKVLIGL
                     EDAGTAPRFLTPITTGTAGGKSIALSQVAKLKLTTTQRFTNESVAGKTRKASGITARG
                     TFAPQDILKVASLKKSSSAYKALKKKLGRSNTVILARNISVFNGYGVEQAYNKAIKLT
                     FKVSKKYNGQKVKLVNTHLGKNKTYTAKVKKGKVTFNVKSGLSDFVLGKEYGNISYKV
                     KKSGVSKVKVTWKGVKGAVKYQIANNHTGTGDFKTKHTSIKSARTYFSIGKKANKTYK
                     FKMRYCKIKNGKKVWSEWSKVKTIKL"
BASE COUNT         2332 a         1255 c         1613 g         1832 t
ORIGIN      
        1 tggagccgag gttttctgtc tgggtctcat agatggtgac ctttcatata atactattct
       61 tgacgtgata tataataagg gtataacctc catagtcggc atcccggatc aggtacacgg
      121 actcatgaaa cacaccgcct ctttaaagat gaaaacagtt cttcttagcg gtgaatatgt
      181 gtcacctata gttgcctcgg atatttcaaa gatatgggga tgcagggttt ttgagcatta
      241 tggaatgagg gaaatgggtc tgggatgtgc agtgagctgt catgttttaa aagggtatca
      301 tgtgagagaa acggatatat ttattgagat agtagatact gaatcaagaa aacccgtaaa
      361 cgacgggaca tggggagaga tcgtttttac tacgctgaca agaaagggca tgccttttat
      421 aaggtacaga acaggtgatg aagggcgctg gctaaaggat ccatgtccat gcggaagcgt
      481 tttgaaaaga ctggacagag taagaaaaag gagaatcaaa tgagcaataa agtagtatat
      541 tttgcaagag atctgctgga aaaaggagaa gattttgtta ttgcaagaat tgtagaaact
      601 aaaggttcca ctccccggaa aagcggagca ttaatgctta tgcgttctga cggtactttt
      661 agcggaacag taggaggagg aaggatagaa gctgagacag aaaaattgtg cagggaggcg
      721 tataaaacaa agaaaaaaag cagaatatac cattttgagt taaattcgaa ggaaaaaaat
      781 tctcttgata tgggatgcgg aggcaatgca gatataataa tagagtatat agatgcaggc
      841 gaccctggaa gttttcttga ggattataaa ataagtgata tagcatatat ttttggtgca
      901 gggcatgtgg gtcttgcttt ggaaccggta cttcgtcata taaatttttc tacagtagtg
      961 atagatgaca gagaagatta tgcaaacaga gaaagatttc ctgaagcaac ggatgtcagg
     1021 acgataaaat cttttgaaca ttcatttgat gatataacct gtgacgaaaa cagttatata
     1081 ataatagtga ccagggggca tatgggagat tatgaagttt tgcgtgatgc attgaaacag
     1141 aagccggctt atatagggat gatgggaagt cgcgaaaaaa attcgacgat aagagacatg
     1201 ttaaaagaaa aagacggatt taccgatgca gagatagatc aagtatatgc acccatagga
     1261 gagaacatat atgccgaaac ccccgaagaa atagctgtca gcatagcggc tgagctgata
     1321 agagtaagaa ccggtaacgg caccagataa tttctcagaa ataatgaaaa agaattttta
     1381 ctgttacaag agtattatac ataaaaagga aaaaagggta aaagaataaa atgataaata
     1441 aaagaaaagt aggagcagtg atacttgcgg cgggatactc ttccagaatg gaaaagttca
     1501 aaccactgat gctcatagga gaaaaaactg cagctgaaag gatcattgac agtgcccgct
     1561 cggcatttgt tgatgatatt gttttagtta ccggatatga gagagaaaaa ctgatacctg
     1621 taatagaaaa aagcggcata agggaggcat ataatcctaa atatgataag gggatgttca
     1681 catccataaa agcgggcata gaagcattgg gagatgaact ggatggattt tttattgttc
     1741 cggtggactg tccactggta atgaaagaaa cttttgaaat gcttgtttct gaaagtcccg
     1801 aattcttcac agttcccact tacatgggaa aaaaaggcca ccccctttat gtgccaatga
     1861 tatacaaaga agaaataatc aaaagcgaaa gcaaaaccgg tcttaaagcc atagtggata
     1921 tttacggaga taaacttata ctgaaagatt ccgccagcga aagcgtggtc cttgacatgg
     1981 acacgaatgc ggactacagg gatctgcttg aatactacag tgaaggatgc agagaaaaag
     2041 accttggtga actggctgcc ggcaggagat tcatttttgt aaggcatgga aaaacggagc
     2101 agcataaaga aaaaatattc ctgggacaga cggacatacc aatgagccag aagggtacag
     2161 agcaggtcga gatcgctgct tttgagatca gcagactcaa actaatgaca ggccatattt
     2221 attcaagtga tcttaaaaga gcttctgaaa cggcagagat catatgcggg atgacagtaa
     2281 tcagtgcggt catatacgaa aaagattttc gcgaaatgtc cctgggagaa tgggacggaa
     2341 aattcataag ctctataaaa gaaaggtatc cggaggagta tgaaaaacgg ggaaaggata
     2401 ttttcagata taaatttgat catgccgcag aaaacttttt tgacctccag tacagagtaa
     2461 taaaaaggct caagaaaata ttggagcagg atcacaggaa agatatagta ataacggctc
     2521 acagtggtgt tatacgtgtc attgaaaaca atctaaaggg tctggatgta agcgatgaat
     2581 gggagccact tgaaaacggc gccttgaggg tgatagaaat gccggaaaga aaaagcccct
     2641 tataaagtct tcaggcattc ctgagaagcc cggcaagaaa tcttttctta tactttatct
     2701 tggctatttt actgattttg ctcacccatg agtaattgaa cacggcaccg atagatccga
     2761 cgatagtgat tccctggata aatttcgcta caaggaggga agtggccagc atattggaag
     2821 tgtcccttat atcctcttct gtaagaacat cttgggggat ttctccacag tcgattttct
     2881 ctccgagaag gtcgcattca tgagatgcta ttattttgcc cgcttctttt gaagcggcaa
     2941 tttttattat tgagaggatg tatgctcttt cggcatccga gtcataatcg aaaccatagc
     3001 ttattgctat ttcgtatatt gatttgaaaa tgactcccag aaataccggg atatccggaa
     3061 ggccgatacc gaagactccg agagcagcac cttcaactgt tgaaagactt ttgttgccaa
     3121 ttgatttgtt cccggcggca acgtcgaaaa ttttgagagt ttctttgttt atgcccatct
     3181 gaagttcata tctgttcaaa gccagttgag cctttatctg atcaaagttg ccggtttttt
     3241 caataaggcc ggtccctttt tcaaatatga aaaaaaaacc tttttcaaag gctgtttcca
     3301 gggcagacaa agctttctcg ggtatttttt gtcttatgcc ggaaaaaaca tctatattca
     3361 ttttgctgtc agcccggtag gagaggtact ttttttcttt tttttctgcc atggccagtt
     3421 gacgacgcag aagtcttttt tcacttgctt tcatattact ttaccagacg tatcctttct
     3481 ccgatagatg ataatctgaa tgtatctttt ccgatttcac tgaatttttc gggaggaatg
     3541 ttttctgtta tcagtccgcc cagttcatct gccccatatc caaaaagaac aggggccatg
     3601 ggagtgctgg ggccaagaac gacggtgcgg gcatttctgc tgatttcaag aagacgaaac
     3661 agggttttgt ttataaaagc cgagcctgtt atgaaaacaa aatcctgata aggcagcaca
     3721 tattcgcagg ccgtatcagg cagatcgccc ttgtcgggtt ttctttcaag gacaattaaa
     3781 ttctctgcgc ttttcagaac tctttccagg gctgtgaaat gaccgataac agcaactttt
     3841 ttgccggaaa cagcacggga ataatcgtca aaggtattac cggtatgtat catgattcct
     3901 gtattttttt ttgagttttc gtcataggtg ccgaaagttt tgcgtatttg ctccgggctg
     3961 tttatataag cattaagggc ggcagtccct atgctggcca gagggaaatc ccatgatctg
     4021 caaagagaag ccacatcctt gagactttgt ccgataaatc tttttctttc aggtaaagga
     4081 gcattttgtt catttaccgt aacggctata ccgcacagtt cccccgcctg aacaatggtc
     4141 caaaccgggc cgctgtggac atcggtaact tctatatcgt ccggtagcga tcctattaaa
     4201 gtgtcataag ctttccacat aaaaattcct ctatatatct ttttgagttt tcatcaaaag
     4261 gtatgatgcg agaatcaaaa cgatcaataa gatcttttct cagctgcgca ttaaggtctg
     4321 ctactatacc gggatatccc gcctccctgc tcataaattt tgctttttca ttcagtttga
     4381 tgactcctat gcaggaaata tttcccccga gaaccttata aagttcattt ctgaaagcag
     4441 gcaccagaag ttccgagcct cctatctcat ccagcagtat cagttttttt ttgcgggctt
     4501 ctttcaggag tttgacaccg cacttttcaa acacttccat atctttttgt gcacctttat
     4561 cgctgcgcac ggcaaaaatg tgttcgagat ccggagaata agaagcttcc acatccaaga
     4621 tttccgcatc tgttatgcgg taagcaaaag cctcaccgtt ttcgtcaata aggcgctggc
     4681 tgcagaatcc tcctatatta tgcctataag gtctgaggta atccctcaaa agtgtggatt
     4741 tgcctgttct tatctgacct tcaagaaaca agtgcttttt catgatttga ttttaacaca
     4801 atgaacatat aattgaaaga aaacaaaaac agaaaacgat gttgttggaa caacatttat
     4861 gttatcatat atcacagaaa ggaaaaccgg gaaagattcc ggtaacggac aaatggaacc
     4921 aaaggaaaaa atagaaataa tttcaagatc cccccttttt gaaggattat caaaggaaga
     4981 gtgtctgaca acctatgagg atattaaacc tcagacaaga aagtattccc gcaatgagat
     5041 aatagtcgga gagggagaga gaatgaactt cataggtata gtagtaagcg gcaaggtgag
     5101 gggcgagaaa ttccatatgg agggggacgt gcatcttgtt tacatgtatg aaacagaaga
     5161 gctgttcggt cttgatacgg ctgctacaag gaccaggatc gcacccgtga cctttcttgc
     5221 ggataagaat tcctcggtgc ttactgtaaa catggacaga ataatgggat cttccatgca
     5281 gggcaaactg gtcttgaata tggtaagaat gctggcagac gaaaatataa ggaagttata
     5341 taaaattgaa gccatatcca gaagggggtt acgagacaga ataatgacat atctgaatat
     5401 aagacagaaa aagctgggta gaaatgaatt tcgtataatg atgactcagg agcagtttgc
     5461 acagtacctt tgtgttaaca gaagcgcgct gacatgggaa cttgcaaaga tgaaaaaaga
     5521 aggtataata gatttttcca aagacagttt tatactaaag gaatgagatg aataaatata
     5581 aaaaaatttt tacaatgttg gtataacaac aaacaaccct gccaaggcag ggtattttat
     5641 tagtataagg tcgtcagcga ggccttactc ttattcaaaa atattttgcc tcatatcagc
     5701 cggtggatat tatccgccgg ctgatgaaac aaaaaaagcg acaggggaag ggaagaaagg
     5761 aaagatatta tggagttttt cgatatggtc caaagaaaac gtgccagcaa agtcatagcc
     5821 atattcctgg caatatgcct gatatttacg atgggcatgt atgtaacaac ggatattgca
     5881 aagggagttg aggatgagcc aagagcaaga aacggagaag actggacttc cgccggtgca
     5941 cctgatttaa gtgcccttcc cgaaaacttg tttttgatag aagtaacagg tccggcaggt
     6001 ataaaaacgg ttcaggtcaa gaaatctaat atcctgacac tgagatttta tcagggcgca
     6061 tataagtggg ggagcttaga caaacaacaa gcgaccggaa caggtattag cctgggagat
     6121 gtatttaagg cagagggcat tgatactgcc ggtataacta aatttgaaga atttgcaaca
     6181 gacaatacgc catcaggcga tttggtgtcc gatatcaatg gcaaatacaa caaagtgctt
     6241 atcggattgg aagacgcagg tacagcacca aggtttttaa ctcctataac tacaggcacg
     6301 gcaggcggca agtccattgc tttatcccaa gttgcaaagt taaaattgac cactactcag
     6361 agattcacaa atgaaagtgt agccggtaag acacgtaaag cttcagggat aaccgccagg
     6421 gggacttttg cacctcagga catcctaaaa gttgcatcgc tgaaaaagag cagctcagca
     6481 tataaggcct taaaaaagaa actgggtagg agcaacacag tcatactggc acgaaatatt
     6541 tcggttttta acggttatgg tgttgaacag gcatacaaca aagcgatcaa actgacattc
     6601 aaagtatcaa aaaaatataa cggacagaaa gtgaagctgg tcaataccca tttgggaaaa
     6661 aacaagacat atactgccaa agtcaaaaaa ggaaaagtta ctttcaatgt aaaaagcgga
     6721 ttatctgatt ttgttttggg taaggaatac ggaaatataa gctacaaagt gaaaaaatcc
     6781 ggggtttcca aagtcaaagt aacatggaaa ggcgtcaagg gcgcagttaa atatcagatc
     6841 gcaaacaacc ataccggaac cggggatttc aaaacaaagc atacttcgat caaaagcgcc
     6901 cgcacatatt tcagcatagg aaagaaagca aacaaaacat acaaattcaa aatgagatac
     6961 tgcaagataa aaaacggtaa aaaagtatgg agtgagtggt caaaggtaaa aactataaaa
     7021 ctgtaagaac ag
//
LOCUS       DAONHK010000050         7020 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_8300_length_7020_cov_4.671931, whole genome shotgun sequence.
ACCESSION   DAONHK010000050 DAONHK010000000
VERSION     DAONHK010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 7020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7020
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8300_length_7020_cov_4.671931"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(157..933)
                     /locus_tag="PLM92_03010"
     CDS             complement(157..933)
                     /locus_tag="PLM92_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006000544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HPR24142.1"
                     /translation="MYKNLKYEIKEGIAYITVNRPDAMNALNNQVLDELFTAFYSVSK
                     DPLVKAVILTGEGKAFVAGADIAAMHKMDPKEGRDMMIKGHNVMNLMETVEKPVIAAV
                     NGFALGGGCELAMACDIRIASERAKFGQPEAGLGIMPGFGGTQRLPRLVGRGMAKYLI
                     MTGEIINAEEAQRIGLVEMVSSAGELMEKAEKTAKKIISNAPLAVGAAKTAINTGYDL
                     DMKTASVLEIETFTGPFASEDKNEGMGAFLQKRTPDFKNK"
     gene            complement(1017..1655)
                     /gene="thiT"
                     /locus_tag="PLM92_03015"
     CDS             complement(1017..1655)
                     /gene="thiT"
                     /locus_tag="PLM92_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006598304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HPR24143.1"
                     /translation="MSSFDFQDFFESTAGQITSAALIIILFIAILFSGKNKKPDTRAL
                     VLSAVFVALAVALNQVKLFHMPQGGSVTAFSMLPIVLCGYFLGVRRGLMAGMCMGIID
                     LIFGPYIIHPVQLLLDYPLAFGALALGAVFREKKGGLYLSYVIGVLCRYVCCVISGII
                     FFGEYAPEGFNAVTWSLWYNLTFLGAECIITLAVLFVPVVHKNIYMLKSELL"
     regulatory      complement(1699..1825)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            1979..2257
                     /locus_tag="PLM92_03020"
     CDS             1979..2257
                     /locus_tag="PLM92_03020"
                     /inference="COORDINATES: protein motif:HMM:NF013286.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HPR24144.1"
                     /translation="MKEKIKNVLEEKVNPVLGEHYGGAVLTGFENGIAKVRLTGACAA
                     CPSAQYTIEDVVKQILMENCKGVKDVVLDTSVSEDLIDMAKKILNKEI"
     gene            2352..3824
                     /locus_tag="PLM92_03025"
     CDS             2352..3824
                     /locus_tag="PLM92_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_155758543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HPR24145.1"
                     /translation="MLMTGDQYIESLRKLGTRVYMFGEKIDNWVDHPMIRPSINCVKV
                     TYDLAQDPQHADLMTAKSNLTGETVNRFGHLHQSTDDLRKKVKMQRLCGQKTASCFQR
                     CVGMDAFNAEFSTTFEIDKKYGTHYHENFKKYLEFVQNNDLTVDGAMTDPKGDRGLAP
                     HAQKDPDLFLRIKEKREDGIIVRGAKCHQTGSINSHEHLIMPTQAMGEDDKAYAVSFA
                     CPSDVEGLYMVYGRQSCDTRKMEPNADVDLGNKNFGGQEALVVFDDVFIPNDRIFMCE
                     EYEFAGMLVERFAGYHRQSYGGCKVGVGDVIIGAAALAADYNGANKASHIKDKLIEMT
                     HLNETLYCCGIACSAEGYPTESGNYQIDLLLANVCKQNVTRFPYEIVRLAEDIAGGLM
                     VTMPSSIDFNSDLKVGKNGETLSEVCNKFYAAAETCTTIERMRILRFLENICLGSSAV
                     GYRTESMHGAGSPQAQRIMISRQSNINAKKELAKEIAGIK"
     gene            3903..4196
                     /locus_tag="PLM92_03030"
     CDS             3903..4196
                     /locus_tag="PLM92_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005837816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPR24146.1"
                     /translation="MICGVRFCGGCNPRYDRGKAFEKIKEQNKDVDFKIAEEGETYDH
                     LLVIGGCTNCCASHCQFSTREGITRMWDEKHIDEVLKKIDEIKSKEKTKQNIK"
     gene            4222..5538
                     /locus_tag="PLM92_03035"
     CDS             4222..5538
                     /locus_tag="PLM92_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HPR24147.1"
                     /translation="MDWKKIYEERKCSADEAVKLVKSGDRVVFAHAVAEPLVLVEALI
                     ANKEAYRNVTISHMVTLGKGEYSLPENKEHFTYEGWFTSPSTRKSLEEGHGEFVPVFF
                     HEVPKMIRNDIFHVDVMFVTVAPPDENGKCCIGVSADYTMQAAKSADIILAEVNDQTP
                     YVYGDCFIDVEEIDAFVETSHPLFELKPSKIGPTEEAIGKHCASLVEDGATLQLGIGA
                     IPDAVLAQLGHKKHLGIHSEMFSDGVVDLYEKGVFDCSEKSFHKGKMIVTFLMGTKKL
                     YDFVDHNPMVEMMPVDYVNNPEIIAKNSKMVSINACVQVDFMGQIVSECIGPKQISGV
                     GGQVDFVRGATMAHDGKGKAIIAMPSVTVKKDGSMVSKIVPYIDHGAAVTTSRCDADY
                     VVTEYGVACMKGRTLHDRARQLIDIAHPDFREELKVQFKERFKIDY"
     gene            5591..6715
                     /locus_tag="PLM92_03040"
     CDS             5591..6715
                     /locus_tag="PLM92_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyrate dehydrogenase"
                     /protein_id="HPR24148.1"
                     /translation="MQALKLVPTIHYFDTFKAFAEEFKLGKGDILVTNQWMYDPYVKP
                     HGIDVPVIFQEKYGKGEPSDEMMDAIKKDMDAYDYERIIAFGGGTIVDICKVLALDVP
                     DKVIKLYTGEVAPKKSKKLVVVPTTCGTGSEVTNVAIAELKALHTKKGIAVEETFADS
                     AVLIPESLQGLPDYVFATSSVDALIHAIESYLSPKASPLTEMYSLKAIEMIMTAYKEI
                     MARGGNSAENRKDLLKDFLFASNYAGIAFGNAGCAAVHALSYSIGAAFHVPHGEANFQ
                     FFTETFKMYMRKDPDGKISNANRIFADILGCDLKDVYPELDKFLGNFLEKKALKDYGM
                     TEAQIDEFTKSTVDNQQRLLGNNYVFLTDDEIREIFANLY"
     gene            6814..>7020
                     /locus_tag="PLM92_03045"
     CDS             6814..>7020
                     /locus_tag="PLM92_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPR24149.1"
                     /translation="MERQLIIIEPCAGLRARGREALAGRWKEGAIAAAIFMACTQIPV
                     YLLDSLFGRVYYAFGEEITRGTALS"
BASE COUNT         2259 a         1355 c         1605 g         1801 t
ORIGIN      
        1 gaaaagcttg tgaatcatgt cagcagaata atcgaaaaca caaaaatact tgagatctca
       61 agccacctaa aaataaagga catcctatga tgtccttttt tatggcactt gaaaagttcc
      121 ggatctggca ttgtcttttg gccggaactt ttaaaattac ttatttttga aatccggagt
      181 tcttttctga aggaaagcac ccattccttc atttttgtct tctgaagcaa agggtcccgt
      241 aaaggtttct atctccaata cagatgcggt tttcatgtcc agatcatagc ctgtgttaat
      301 ggctgttttg gctgcaccga cggccagtgg agcattgctg attatttttt tggctgtttt
      361 ttccgctttt tccataagct caccggcaga tgagaccatt tccacaagac ctattctctg
      421 tgcttcttcc gcatttatta tttcacctgt catgattagg tattttgcca tgcccctgcc
      481 cacaaggcgg ggaaggcgct gagtgccgcc gaaacccggc attataccaa gacctgcttc
      541 cggctgtccg aatttggctc tttcggaagc gatccttatg tcacaggcca tggccagttc
      601 acagccgcct cccagagcaa agccgttaac agcggcaatt accggtttct ccacggtttc
      661 catcagattc ataacattat gaccttttat catcatgtca cggccttctt tgggatccat
      721 tttatgcatt gcagctatgt cagctcccgc aacaaaggcc ttgccttcgc ctgttaatat
      781 cactgcttta accagcgggt cctttgatac actgtaaaaa gcggtaaaaa gctcatccag
      841 gacctggttg ttcagagcat tcattgcatc cggcctgttt acggttatat atgcgatacc
      901 ttctttgatt tcgtacttaa gatttttata cattttctta tcctttctgt gtttttatcc
      961 aagataatta tataacattt tgtccgaaaa tcaaatgtat tcggagtacc ggaaagtcat
     1021 aataactctg atttcaacat gtaaatattt ttatgcacga caggaacaaa aaggactgcc
     1081 agcgtgataa tacattctgc acccagaaag gtcagattat accacaggga ccaggtaact
     1141 gcattgaatc cttcaggagc atattctccg aagaatatga tgcccgaaat cacgcagcag
     1201 acatatctgc ataggacacc gataacatat gacagataaa gtccgccttt tttttctctg
     1261 aaaaccgctc cgagcgccag ggctccgaaa gccagcgggt aatccagaag aagctggacg
     1321 ggatggatta tataaggacc gaatatcagg tcaattatgc ccatacacat tccggccatg
     1381 aggcctcttc ttacaccgag aaaatagccg catagaacta tcggcagcat ggagaatgca
     1441 gttactgaac cgccctgagg catatgaaaa agctttacct gattaagagc cactgcaaga
     1501 gccacaaaaa cggcagaaag gaccagtgcc cttgtgtccg gttttttgtt tttgcctgaa
     1561 aaaagtatgg caataaacag aataatgatg agtgccgcag aagttatctg gccggctgtg
     1621 gattcaaaga aatcctgaaa atcaaaagat gacataaaaa acctccttta gtatattagg
     1681 aggggaaaga tacattgagc atcttgatac ttgcttccct gcgccggcat tatccggatc
     1741 aggtcttgag ggtaatccgt cagaaaagta tgtttgatca tgccttttcc cggatatctc
     1801 agcaaaagct cccctagcca tattattaat aaaaaaagca tataacagcg atcatataaa
     1861 gtcaagaaca aagggtattg agttcctctg catattttcg tatatatgcg caaaatcatt
     1921 ctattgaaaa aaacactgcc ctgaagtata ataataacat gagacatcgg aggagatgat
     1981 gaaagaaaaa ataaagaatg tacttgaaga aaaagtaaat ccggtactgg gagagcatta
     2041 cggaggcgct gtacttacag gctttgagaa cggaatcgca aaagtgagat taactggagc
     2101 atgtgcggca tgcccgtcgg cccagtacac gatcgaggat gtggtaaaac aaatattgat
     2161 ggagaactgc aaaggggtaa aagacgttgt tttagacact tcagtgagcg aggaccttat
     2221 agatatggca aaaaaaatat tgaataagga aatatagcaa gcagggtcgt atttggtacg
     2281 gtacttgcat attatgatgt cagtaagtaa ataaaatgtt tacatataat tacaaaagga
     2341 gtgattaaat tatgttaatg acaggcgacc agtatatcga aagtttaaga aaactcggaa
     2401 caagagttta catgttcggt gaaaagatcg acaattgggt tgatcacccc atgatcagac
     2461 cttcaatcaa ctgtgtgaag gtgacttatg atctggctca agatcctcag catgccgatc
     2521 taatgacagc caagtccaat ctgacgggag agaccgtaaa caggttcgga catctgcatc
     2581 aaagcacaga tgatctaaga aaaaaggtaa agatgcagag actttgcgga cagaaaacag
     2641 cttcatgttt ccagagatgt gtaggaatgg atgcttttaa cgcagaattt tcaacaacat
     2701 ttgagataga caaaaaatat ggaacccatt accatgaaaa tttcaaaaaa tatctggaat
     2761 ttgttcagaa taatgattta acagttgatg gagccatgac agacccaaaa ggtgacagag
     2821 gtcttgctcc ccatgcacaa aaggatccgg acctttttct tcgtatcaaa gaaaagagag
     2881 aagacggtat catagtaaga ggagctaaat gccatcagac aggttccata aactctcacg
     2941 agcacctcat aatgcccaca caggccatgg gagaggatga caaagcttat gcggtttctt
     3001 tcgcatgtcc ttccgatgtt gaaggcctct acatggtata tggcagacaa tcctgtgata
     3061 caagaaaaat ggagccgaat gctgacgttg atctgggcaa caaaaacttc ggcggtcagg
     3121 aagccctggt agtatttgat gatgtattta ttccaaatga caggatattc atgtgtgaag
     3181 aatacgaatt cgcaggaatg ctggtagaaa gatttgccgg atatcacagg cagtcatacg
     3241 gcggctgcaa agtaggtgtc ggtgatgtta taataggagc tgctgctctt gctgccgatt
     3301 acaacggtgc caacaaagct tcccacataa aggacaagct tattgaaatg acacatttga
     3361 atgagaccct ttactgctgc ggtattgcat gctctgctga aggatatcct acagaatcgg
     3421 ggaattatca gatagatctg ctgctggcca atgtctgcaa gcagaatgtt acaagattcc
     3481 cttatgagat agtaagactg gcagaagaca tcgcaggagg gcttatggtg accatgccgt
     3541 cttccattga tttcaattca gacctgaaag tgggcaagaa cggcgaaacc ctttctgaag
     3601 tatgcaacaa gttctatgct gcagctgaaa catgcaccac catagaaaga atgaggatac
     3661 tgagattcct tgagaatatt tgtctcggat cttcggcagt aggatacagg acagagtcca
     3721 tgcacggagc cggatcacct caggcacaga gaataatgat ttcccgtcag agcaacataa
     3781 atgcaaaaaa agaactggcc aaggaaatag caggaatcaa ataacaggaa gatcaaagat
     3841 cattgagccg ctgggctttc cgttcagcgg ctcattatta aattaaaatt atggagagcc
     3901 atatgatatg tggagtaagg ttttgcggag gatgtaatcc ccgctatgac agaggaaaag
     3961 cttttgaaaa aataaaagaa caaaacaaag atgtggattt taagatagcg gaagaaggag
     4021 aaacatacga tcatcttctt gttataggag gttgcacaaa ttgctgtgca tcccattgtc
     4081 agttttcaac cagagaaggc attacgagaa tgtgggatga gaaacatata gatgaagtgc
     4141 ttaaaaagat cgatgagata aagtcaaaag aaaaaacaaa acaaaatatc aaataaaaaa
     4201 tgaaaggtca ggtaatttta aatggactgg aaaaagattt acgaagaaag aaagtgcagt
     4261 gccgatgaag cggtaaaact ggtcaagtcc ggagacagag tcgtatttgc acatgctgtt
     4321 gcagaacctc ttgttcttgt ggaagcactg atagcaaaca aagaggctta caggaatgtt
     4381 actatttccc atatggtgac cttgggaaaa ggtgaatact ctctgcctga aaacaaagag
     4441 cattttactt atgaaggatg gttcaccagc ccttcgacaa gaaaatccct ggaggaaggg
     4501 catggggaat ttgttcccgt atttttccac gaagtaccga aaatgataag aaacgatatt
     4561 tttcatgttg atgtaatgtt tgtaacagtg gcaccgcccg atgaaaacgg gaaatgctgt
     4621 ataggagttt ccgccgatta tactatgcag gccgcaaagt cggcagatat tatcctggcc
     4681 gaagtaaatg atcagacacc ctacgtttac ggagactgtt ttatagatgt tgaagagatc
     4741 gatgcctttg tagagacttc tcatcctctt tttgagctaa aaccctcaaa gataggtccc
     4801 acagaagaag caataggcaa acactgtgca agtcttgttg aagacggagc cactcttcag
     4861 ctgggaatag gagccatacc cgatgcagtg ctggctcaat tagggcataa gaaacatctt
     4921 ggtatccatt cggaaatgtt ttctgacggg gtagttgatc tttatgaaaa aggtgtgttt
     4981 gactgttccg aaaaatcatt tcacaaaggg aaaatgatag ttacattcct tatgggcaca
     5041 aagaaacttt atgactttgt agatcataat cctatggtgg agatgatgcc tgtagactat
     5101 gtcaataatc cggaaataat tgctaaaaat tcaaagatgg taagtattaa tgcctgcgta
     5161 caggttgact ttatggggca gatagtttct gaatgtattg ggcctaaaca gatttccgga
     5221 gtagggggtc aggtagattt cgtaagggga gccacaatgg ctcatgacgg aaaggggaaa
     5281 gcgataatag caatgccttc cgtaacagta aaaaaggacg gaagcatggt atcgaagata
     5341 gttccatata tcgatcacgg agcagcagta actacatcaa gatgtgatgc cgattatgtg
     5401 gtgaccgaat acggtgtggc ttgtatgaaa ggcaggaccc ttcatgacag ggcaagacag
     5461 cttatcgata tagcacatcc tgatttcagg gaagaactga aagttcagtt caaagaaaga
     5521 ttcaagatag attattaagg cgcagggcgc cggataagta ccgcaaacaa gcggtgaaaa
     5581 ggaggaaaaa atgcaagcat taaaattagt accaacgatc cattattttg acacattcaa
     5641 agcatttgca gaagagttca aacttgggaa aggcgacata cttgttacca accagtggat
     5701 gtatgatcct tatgtaaaac ctcacggcat agatgtgcct gtgatttttc aggagaaata
     5761 cggaaaaggc gaaccttctg atgaaatgat ggatgcaatc aaaaaagaca tggatgccta
     5821 tgattatgaa agaatcatag cattcggagg cggcactata gtggatatct gcaaagtact
     5881 tgccctggat gtacctgaca aagtaataaa gctttatacg ggggaagtgg ctcccaaaaa
     5941 atcaaagaaa cttgtcgtag ttcctacaac atgcggtacg ggctcggaag ttactaatgt
     6001 tgccatagca gaactcaagg ctctgcatac aaaaaaaggt attgcagtag aagaaacttt
     6061 tgctgattct gcagtactta ttcccgaatc tctgcaggga ctgcccgatt atgtatttgc
     6121 cacaagttcg gtagatgcac ttatacatgc cattgaatca tatctgtcac caaaggcatc
     6181 accgctgaca gaaatgtact cactgaaggc catagaaatg ataatgacag catacaagga
     6241 gataatggca agaggcggca acagtgcgga aaacagaaag gatcttctga aggatttcct
     6301 gttcgcatca aactatgcag gaatagcatt tggaaatgcc ggctgtgccg cagtacatgc
     6361 actttcctat tccatcggtg cagctttcca tgtgccccac ggagaagcca acttccagtt
     6421 cttcaccgaa acattcaaaa tgtacatgag aaaagatccc gacggaaaaa tctcaaatgc
     6481 aaacaggata tttgctgata tactgggttg tgatcttaag gatgtttatc cggaacttga
     6541 taaattcctt ggcaacttcc ttgagaaaaa agcacttaaa gattacggta tgacagaagc
     6601 acagatagac gaattcacaa aatccaccgt tgacaatcag cagagacttc tgggaaacaa
     6661 ctatgtattc cttacagatg acgaaataag agagatcttt gcaaatcttt attgatctta
     6721 aaggcaggca gccgccccgc atattgcatg gggcggcttt ttattgtatt attatagaat
     6781 agtgtttttt atcagagagg agaaaaaaag agtatggaaa gacagctgat aataatagag
     6841 ccatgtgccg gacttagagc gaggggacgt gaagcgttgg caggcagatg gaaagagggg
     6901 gccatagcag cggccatatt tatggcatgc acacagatac ccgtttattt gctggattct
     6961 ttgtttggta gggtttatta tgcctttggg gaggagataa ccaggggtac agctctttca
//
LOCUS       DAONHK010000051         6950 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_8407_length_6950_cov_4.785642, whole genome shotgun sequence.
ACCESSION   DAONHK010000051 DAONHK010000000
VERSION     DAONHK010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6950
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8407_length_6950_cov_4.785642"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            28..822
                     /locus_tag="PLM92_03050"
     CDS             28..822
                     /locus_tag="PLM92_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPR24150.1"
                     /translation="MLAQLMKYEFKAVGRIMLPLYGAWLIMSLLMGLAVTILEKVVAL
                     GIIIGFLYGAVTIACVVLTIVMVIQRFYKNLLGSEGYLMFTLPVKTANHVWNKVISAS
                     FWIFAGVVMGLLSMGIIVLFTEGYTESFEAISMFFKIVTGFLGVPKVVLLIVEIILLA
                     LLMTGEAALKIYAAISLGHQWGNHRILGAIGAYIGFGIVEVIITNIIFNLFPNMEQDL
                     ERLFESTSDFMEMQLMIMVMALIVLMLGTVYFLVTYKLLDRRLNLE"
     gene            complement(833..1567)
                     /locus_tag="PLM92_03055"
     CDS             complement(833..1567)
                     /locus_tag="PLM92_03055"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HPR24151.1"
                     /translation="MFKNIKNKTVTCAEMKVLEKTADAAGLSYYQMMENAGTAAFTEI
                     LKNIPGPIREIRASVLCGKGNNGGDGFVVARLLNESGARVTVLLTEGSPVTKDAITNF
                     DLLKKQNINIIDIFSSGENCNDSSLEEKISTEVLSYLKNSHIIIDSIYGTGFRGRLRP
                     SSEALINAVNNASAEGALIFSLDIPSGLPGDADKNQTDFGKCINASFTIVFHAKKPVH
                     NNKTALLTMGSLIVADIGIYDAIGKG"
     gene            complement(1623..3242)
                     /locus_tag="PLM92_03060"
     CDS             complement(1623..3242)
                     /locus_tag="PLM92_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017300642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HPR24152.1"
                     /translation="MAKAKSDLSKLQNGSDIRGIALTGIEGELPNLCADEAIKIARGY
                     LVWLCKKTGKRPDELKIAIGRDPRMSGQSLFHSLNTGMGPYGVQVLDCGIASTPAMFM
                     STLFPDFECDGAIMITASHLPFNRNGFKFFDKAGGLNREDISEIIETAKGGKKKLEPL
                     GPAVFEDNFFMDLGKKTYPSQPADLMKEYCHHLRKIIIDRVGEGPEPLKGLKIVVDAG
                     NGSGGFYANDVLKPLGADTTGSIFLEPDGTFPNHAPNPEDRDAMAAISMAVLTTGSDL
                     GLIFDPDVDRSSAVDGRGREISRNRIVALAAAIVAEEHPGTTVVTDSITSTQLTEFLE
                     RDLGLSHFRFKRGYKNVINKAIQLNKEGRDAQLAIETSGHAALKENYFLDDGAYLATL
                     IVVKAAKLAKEGKTPDSLIENMKDPEEAREVRMNISREDFGPYGDTVLDDLKKWISVG
                     LTDACATSVHSKEASPAPVGMSPEEPNYEGVRINFDKDNGDGWCLLRKSLHDPLMAMN
                     MESNTKGGCKIISGKMRSFLKNYPDLDISKL"
     gene            complement(3223..4056)
                     /gene="larE"
                     /locus_tag="PLM92_03065"
     CDS             complement(3223..4056)
                     /gene="larE"
                     /locus_tag="PLM92_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010234085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HPR24153.1"
                     /translation="MQNKLLLLKQKITAYGTLAVAFSGGVDSAFLLSYAKEVLGEGVI
                     AVTAMAPNFDPKEISGAKKFCEEKDIRHLIINLGPELIAKFCHNPEDRCYICKKEVFS
                     KLRSTVNDLGYYNLADGTNRDDMDDFRPGQKALTELKIASPLKEAGFTKADIRQGLKA
                     MNIDMWDKPAFACLASRIPYGEEITVKKLKSVNDAESALMDLGFRQVRVRHHGDVARI
                     EVLPEERNLFYNEKFMDHVNAAVKKAGFKYASLDLSGYKMGNLNKETDKEYKKHGKSK
                     K"
     gene            complement(4056..4619)
                     /locus_tag="PLM92_03070"
     CDS             complement(4056..4619)
                     /locus_tag="PLM92_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016206885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HPR24154.1"
                     /translation="MTEREKAMELLKKYNREEFHIRHALTVEGTMRYFAEKRGYDPDF
                     WGMVGLLHDIDFEMYPDEHCKKAPELLSEAGFGEEFIHAVVSHGYGLCGDTKPTHEME
                     KVLFATDELTGLIGACALMRPSKSTKDMEVKSVRKKFKDKKFAAGCDREVISSGAEEL
                     GIELDELFEETIMAMRSCEDDVNWYFE"
     gene            complement(4616..5539)
                     /locus_tag="PLM92_03075"
     CDS             complement(4616..5539)
                     /locus_tag="PLM92_03075"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPR24155.1"
                     /translation="MNKKIKSTIFLLLCAILWGVAFVAQRDGMADMGPFYFSAFRMYM
                     GSLTLIPIIYLGDRLKKHEQGKSGMPEQTSVQKAAEKGYLIRGGVLCGAVIFFAANFQ
                     QVGLVSVSAGKTAFITTLYIILVPLFGMFMKHRPNIFNWTGVILGTVGLYFLCITESL
                     SIAFGDLIILIGAFFWAAHILCIGFFAPKVDVAKLTCIQFLIAGTISLAVAIFREPIS
                     AEIFVGQLPNLFFAGVLSCGLAFTFQALGQKDANPTVASIILSTEALFGALGGFIFLG
                     ESFTQRELIGCILMFAAIIIAQLPSRKEKSQ"
     gene            5771..>6950
                     /locus_tag="PLM92_03080"
     CDS             5771..>6950
                     /locus_tag="PLM92_03080"
                     /inference="COORDINATES: protein motif:HMM:TIGR01976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase-like protein"
                     /protein_id="HPR24156.1"
                     /translation="MKDFKFDVDYVREQFPALCKTVNGNPAVFSDGPGGSQVPMIVIE
                     RIKEYLLYRNANTHGVFKTSVESDDLYWKAKEAFADFLNCSPQEVAFGANMSSNNFKL
                     ALGLVRTMEPGDEVLITDIDHEGNRSPWRTLEDFGITVKSVAVDPKTATLDMDDFKAK
                     LSDKTKVVAVNWAANACGTVTDVKECIRLAHEKGALTVVDAVHYAPHKVIDVKEIDAD
                     FLHCSAYKFFGPHIGITYIKKEVGEKVKTVRVMADDNEDMPWKFETGTPAFELAWGAA
                     GAVEFIADIGEKHAKYFEDELKGLTGRRKNIVAGMKAIDAYEEPMAKKLRRELAEIKG
                     VKIYGPPEGHPRTSTVTFLAEGINANHIAKKLAEKGIFVWDGDYYAIEIIMNVWKLGD
                     V"
BASE COUNT         1882 a         1529 c         1581 g         1958 t
ORIGIN      
        1 agacggactt ttcagggagg tgttcaaatg ttagcacaat tgatgaaata tgaattcaaa
       61 gctgtaggca ggataatgct tcccctttac ggggcatggc tcattatgtc acttctgatg
      121 gggcttgcag tgaccatcct cgaaaaagtg gtggccctgg gaataataat aggattcctt
      181 tacggagccg taaccatagc atgtgtggtc cttaccatag taatggtaat acagaggttt
      241 tacaaaaatc tgctgggatc tgaagggtat ctaatgttta ctctgcctgt caaaacggca
      301 aatcatgtat ggaataaagt catatcggcc tccttctgga tatttgccgg tgtagtaatg
      361 ggacttttgt ccatgggcat aatagttctg tttaccgaag ggtacacaga aagcttcgaa
      421 gccataagta tgtttttcaa aatagtgacc ggcttcctgg gggttccaaa agttgtgctc
      481 ctgatcgttg agatcatatt actggcactt ttgatgaccg gggaagcagc actcaaaata
      541 tatgccgcca tttctctggg acatcagtgg ggaaaccaca ggatactggg agccatagga
      601 gcatatatag gattcgggat agtggaagtt attataacga atattatatt caatctgttc
      661 ccgaatatgg aacaggatct ggaaaggctt ttcgaaagca ccagtgactt catggagatg
      721 cagcttatga ttatggtgat ggcacttata gtgctgatgc tcggtacagt gtatttctta
      781 gtgacataca aacttctgga cagaagactc aatctggaat agacgaaaaa tctcagcctt
      841 ttccaatagc atcatatatg ccgatatcgg cgacgataag actgcccatg gtaagaaggg
      901 cggtcttatt gttatgaacg ggtttttttg catgaaaaac tatagtgaag gaagcattga
      961 tgcattttcc gaaatcagtt tgatttttat ctgcatcccc gggaagtccc gaagggatat
     1021 ccagagaaaa gatcaaagct ccttccgcag acgcgttatt gacggcattg atcaaagctt
     1081 cagaagaagg cctgagcctg cccctgaaac ctgtgccgta tatggaatcg atgatgatgt
     1141 gggaattttt caggtatgaa agaacttccg tggatatttt ttcttcaaga ctgctgtcat
     1201 tacaattttc tccggaggag aatatatcga ttatatttat gttttgcttt ttcagaagat
     1261 caaaattggt tattgcatcc ttagtcaccg gggatccttc agtaagtaag acagtgacac
     1321 gggcaccgct ttcgttaagg agccgtgcaa caacaaatcc gtcccctccg ttattgccct
     1381 tgccgcaaag gaccgaagcc cttatttccc ttatcggtcc cggtatattt ttgagtatct
     1441 cagtaaaagc cgctgtgcct gcattttcca tcatctgata atatgaaaga ccggcggcat
     1501 cggcagtctt ttcaagaact ttcatttcag cgcatgttac agttttattt ttgatatttt
     1561 tgaacataaa aacaccttca aaacgatttt aaacttttat ggcgtcaaag accggcagtg
     1621 ttttacagtt tggaaatgtc aaggtcggga tagtttttta ggaaactgcg cattttcccg
     1681 gatattattt tgcacccgcc ctttgtgttg gactccatgt tcattgccat aagcggatca
     1741 tgaagagatt ttcgcagaag acaccaaccg tctccattgt ctttgtcgaa atttattctt
     1801 actccttcgt agtttggttc ttccgggctc atgccaacag gggctggaga agcttctttg
     1861 ctgtggactg acgttgcaca tgcatccgta aggcccaccg atatccattt tttcagatca
     1921 tcaagaacgg tatctccgta aggcccgaag tcttcccgtg atatattcat gcggacttct
     1981 cttgcttctt ccggatcctt catgttttct atgagactgt ccggagtttt gccctctttt
     2041 gccagcttcg ccgcttttac tactattaga gttgccaggt aagcgccgtc atcaagaaaa
     2101 tagttttctt ttagggcggc atgacctgaa gtttctatgg ccaattgggc atcgcgacct
     2161 tccttattga gctggatagc tttattgata acgtttttat atccgcgttt gaagcggaag
     2221 tgggaaagcc ccagatccct ttcaagaaat tccgtaagct gagtgctggt tatgctgtcg
     2281 gtcaccactg ttgtgcccgg atgctcctct gccacgatgg ctgcggccag ggcaactatc
     2341 ctgtttctgg atatttctct tcctcttccg tccactgcag aagaacggtc cacatcgggg
     2401 tcgaagataa gacccaggtc cgagccggtt gtgagaactg ccatgcttat ggctgccatg
     2461 gcatccctgt cttcgggatt gggggcatga ttgggaaaag taccgtcggg ttcaagaaaa
     2521 atgctgccgg tcgtatccgc acccaggggt ttcaaaacat cattggcata aaaaccgccg
     2581 gagccgttgc cggcatccac aacgatctta agacccttta gtggttcagg accttcgcct
     2641 actctgtcta ttattatttt gcgcaggtga tgacagtatt ctttcataag gtctgccggc
     2701 tgtgacggat aagttttttt gcccagatcc atgaagaagt tgtcttcaaa tacggcagga
     2761 cccaggggtt ccagcttttt tttgccgccc ttggctgtct ctattatttc tgaaatatct
     2821 tctctgttca atccgccggc tttgtcaaag aatttgaaac cgttgcgatt aaaaggaagg
     2881 tgtgaagcag ttatcattat cgccccgtcg cattcaaagt ccgggaacag ggtggacatg
     2941 aacatggcgg gagtagaagc gataccgcag tctaatacct gcactccgta ggggcccatg
     3001 ccggtattca gggaatgaaa aagactttgc ccggacatcc tgggatcccg tcctatggct
     3061 atttttaatt catccggcct tttgccggtt tttttgcaca gccagaccaa ataaccacgg
     3121 gcgatcttga tggcctcatc ggcgcaaagg ttggggagct cgccttctat acctgtaagt
     3181 gctattcccc ttatatcact gccgttttgc agtttgctca aatcactttt tgcttttgcc
     3241 atgttttttg tactccttgt ctgtttcttt gttcagattc cccattttat aaccggaaag
     3301 atccagagac gcatatttga atcccgcttt tttgacggcg gcattcacat gatccatgaa
     3361 tttttcatta taaaaaagat tacgttcttc aggaaggact tctatccttg ccacatctcc
     3421 gtggtgacgg acccttacct gccgaaatcc cagatccata agagccgatt ccgcatcatt
     3481 tacagacttc agttttttga cggttatctc ttctccgtag gggatacggg aagcaaggca
     3541 ggcaaaggcc ggtttatccc acatatctat gttcatggcc tttagaccct gtcttatatc
     3601 tgcctttgta aatcccgctt ctttcagcgg gcttgctatt ttcaattcag tgagagcctt
     3661 ctgaccggga cggaaatcat ccatatcatc cctgtttgtc ccatccgcca gattataata
     3721 acccagatcg ttcactgtgg atctcagctt ggaaaagact tcttttttgc atatatagca
     3781 tcggtcttcc ggattatggc aaaacttggc gataagttcg ggacccagat ttattataag
     3841 atgcctgata tctttttctt cgcagaattt ttttgcgcct gagatctctt tcgggtcgaa
     3901 gttgggtgcc atggctgtta cggcgatgac tccttctccc aggacttcct ttgcataaga
     3961 aagaagaaag gcgctgtcaa cgcctccgga aaaggccacg gcaagggtgc cgtaagcagt
     4021 gatcttttgt ttcagaagca aaagcttatt ttgcattact caaaatacca gttaacatca
     4081 tcttcacagc ttctcatggc catgatggtt tcttcaaaaa gttcatccag ttcaatgccc
     4141 agttcttccg caccgctgct gataacttcc cggtcacatc cagcggcaaa tttcttgtct
     4201 ttgaatttct tgcggacgga ttttacctcc atgtcctttg tgcttttgga agggcgcatc
     4261 agcgcacagg caccgataag acctgtcagt tcatcagtgg cgaaaagtac tttttccatt
     4321 tcatgagtgg gcttagtatc gccgcaaagc ccgtatccgt gggacacgac agcatgtatg
     4381 aactcttcgc caaagcctgc ttccgaaagc agctccggtg cctttttaca gtgctcatcg
     4441 ggatacattt caaaatctat atcatgaaga agtcctacca tgccccagaa atccgggtca
     4501 tatccccgct tttctgcgaa ataccgcatg gtaccttcca ctgtaagggc atgtcttatg
     4561 tggaactctt ccctgttgta tttttttaga agttccattg ctttttctct ttctgtcatt
     4621 gtgatttctc ctttcttgag gggagctgtg cgattattat agccgcaaac ataagaatgc
     4681 agcctataag ctctctttgg gtaaaagatt ctcccaggaa aatgaaacct cccagggcac
     4741 cgaaaagagc ttcagtactt aatattatgg atgccactgt gggattggca tctttttgtc
     4801 ccagagcctg gaaagtaaat gcaagtccgc atgacaacac tcccgcaaag aaaaggttag
     4861 gcaactggcc aacaaagatc tctgcggaga tgggttccct gaaaatagcc acagcaaggc
     4921 ttatggtacc cgctatcagg aactgtatgc atgtcagctt ggcaacatct actttcggtg
     4981 cgaaaaatcc tatgcaaagg atatgtgctg cccagaaaaa tgcacctatc agtattataa
     5041 gatctccaaa agctattgaa agactttcgg ttatacataa aaagtaaaga cctacggtcc
     5101 ccaggatcac ccctgtccag ttgaaaatgt tgggcctgtg tttcatgaac atgccaaaca
     5161 gcggcaccag tattatatat agagttgtta tgaatgcagt tttgccggca cttacagaaa
     5221 ccagccccac ctgctgaaaa ttggcagcga agaaaatcac tgcgccgcat aaaactccgc
     5281 cccggataag ataacctttt tcggcggcct tctgaacgga agtctgctcc ggcataccgg
     5341 attttccctg ttcgtgtttt ttcaagcggt cgcccaaata tattatgggt atcagcgtga
     5401 gagatcccat atacatgcgg aatgcactga aataaaaggg tcccatgtca gccatgccgt
     5461 ctctttgggc cacaaaagcc acgccccaga gaatggcaca taaaagcagg aatatcgtag
     5521 attttatttt tttgttcata gtcgcttcct tttatatatg atatttacag taaggcaatt
     5581 atatcataag gaaaagatga tgtatagaag gcataaaagg atcatcaaca gcgaaaagca
     5641 ttggaacaac aatgctcttg tatgatttac aaaaaaaatc cgacattttc aaaacgggga
     5701 caattgtatt tttttggtat cagatataaa gtttaagagg gtatatattt ttatgtgaag
     5761 gagaacgaca atgaaagatt ttaaatttga tgttgactat gtcagagaac agtttcctgc
     5821 tctttgcaaa acagtaaacg gaaatcctgc cgtcttttct gatgggcccg ggggaagtca
     5881 ggtgcccatg atagttatcg aaagaataaa agaatatctt ctatatcgta atgccaatac
     5941 tcacggagta ttcaaaactt ctgtggagag cgatgatcta tactggaaag ccaaagaagc
     6001 atttgcggat tttcttaact gcagccccca agaggtggca ttcggagcaa acatgtcatc
     6061 caacaacttc aagcttgccc tgggactggt aaggaccatg gagccgggag acgaagtact
     6121 tataacagac atagaccatg aggggaacag atccccatgg aggacccttg aagatttcgg
     6181 cataacagta aaaagcgtag cagtggatcc gaagacggca actttggaca tggatgattt
     6241 caaggcgaag ctttctgaca aaacaaaagt agttgctgta aactgggcgg caaatgcgtg
     6301 cggcacagtg actgatgtaa aagaatgcat cagacttgcc cacgaaaaag gtgccctcac
     6361 tgtggtggat gccgtgcatt acgctcctca caaagtaata gacgtgaaag aaatagatgc
     6421 agattttctt cactgctcgg catataaatt ctttggtcct catataggca taacctacat
     6481 aaaaaaagaa gtcggagaaa aagtaaaaac tgtaagagta atggccgatg acaatgaaga
     6541 catgccctgg aaattcgaga ccggtacacc ggcattcgaa ctggcctggg gagctgcggg
     6601 agcggtggaa ttcatagcgg atataggcga aaaacatgcc aaatattttg aagatgaatt
     6661 aaaggggctt acaggcagaa gaaaaaatat agtggccgga atgaaggcca tagatgcata
     6721 cgaagaaccc atggcaaaaa aactccgcag ggaactggcc gaaataaaag gtgtcaagat
     6781 atatggacct ccggaaggac atccgaggac ttccacagtt acattcctgg ctgaaggaat
     6841 aaatgcgaat catatcgcaa aaaaactggc cgaaaaaggc atcttcgtct gggacggaga
     6901 ttattatgca attgagataa taatgaacgt atggaagctg ggagatgtgg
//
LOCUS       DAONHK010000052         6490 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_9214_length_6490_cov_4.233256, whole genome shotgun sequence.
ACCESSION   DAONHK010000052 DAONHK010000000
VERSION     DAONHK010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6490
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9214_length_6490_cov_4.233256"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(7..1041)
                     /locus_tag="PLM92_03085"
     CDS             complement(7..1041)
                     /locus_tag="PLM92_03085"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPR24157.1"
                     /translation="MKKTFCVLLILVLVLTAVSCGGEKTETQKPGTVIEKDSEGFVIV
                     DQAGREVYVPEKVKTVAYCYRVPARVLISLGMGDSITGAGKVDDFLKKVQPSLADVPS
                     VGTGVIDIEALAELAPDVVFHKASDKAGLDAAGDVGVVAIGLSMETQEDIVVALDIIG
                     KVCGTEKKARQFIDYYNDKLKEDKKLTEKIKKKKTVLMMGSSIGKVADGSMLQGFMIE
                     KAGGKNVAADLQATEIWPTAGTEQIFEWDPEYIFISSESTDYNAKDILSNETWSEIKA
                     VKNKHVYEMPAPGDSWEFPGAVSALGIDYMMNKMYPELLGDDALEKNVNDFYDLFYGT
                     TFDREELGYS"
     gene            complement(1055..1864)
                     /locus_tag="PLM92_03090"
     CDS             complement(1055..1864)
                     /locus_tag="PLM92_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007490280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPR24158.1"
                     /translation="MTEQKNTDEKIIYEAKDVSFTYPKGSRQILDRISLVLKKGEILS
                     VLGANGAGKTTLLNCMAGLLKPDHGSIELCGRKTKEMTAKEIAGKAAYVPQLHTPAFD
                     YKVGDFVLMGRAPKKGTFESPGEEDKKIASKVLKEMGIDHLADRSYIEISGGERQQAL
                     IARAMVQEPEVILFDEPTAHLDFGNQHRVLGMIRKMTSEGFAIVITTHNPDHALMLGG
                     KTAIIDRKGKMTQGEASEILTEERLSGIYDTDIRLIYIEEIGRKVCVMPDF"
     gene            complement(1861..2832)
                     /locus_tag="PLM92_03095"
     CDS             complement(1861..2832)
                     /locus_tag="PLM92_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HPR24159.1"
                     /translation="MDTRNGVVTNVIFFAVLLCLMAFCLGIGKYSLNIFGEMSEMDIN
                     MLLGIRLPRVLAAVFVGAALSLAGAVYQGVFKNPLVSPEFLGISTGACVGAAVAILMS
                     FSAIFIQAFAFLGGISAVTLTLLIPKALKSNSNIMLVLAGIIVGGAMSSVLGFIKYIA
                     DPETQLASITYWQMGSFAYVDMNNLLYVLPFAVAAAFLILAMSWWVDILSLGENEAQM
                     LGVSVGVMRGVFIVCATVLTASAVCIAGTIGWVGLVIPHLCRMIVGPANRVLLPSACL
                     LGGIFLLTVDTATRLIGPAEMPISILTGIIGAPFYAWLLYRQRSRLR"
     gene            complement(2958..3614)
                     /gene="phoU"
                     /locus_tag="PLM92_03100"
     CDS             complement(2958..3614)
                     /gene="phoU"
                     /locus_tag="PLM92_03100"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HPR24160.1"
                     /translation="MATRPKMDEELKAINKKVIEMAELIEETIRSSIKSLKEKDVELA
                     RKIIDGDEKIDDMEDEINSDCFKFLATQSPLAGDLRYCISIMKMIRDLERIGDHCEDL
                     AKYTLRLENREYYKELIDIPRMADLAARMVSNAIRAFVEKDLRMARKVWKSDEEVDEL
                     FRYIYEEQLDIAEKNTENARTAISFAFVAAHLERIADYATNICEETVFSMEGRYRMDL
                     "
     gene            complement(3642..4406)
                     /gene="pstB"
                     /locus_tag="PLM92_03105"
     CDS             complement(3642..4406)
                     /gene="pstB"
                     /locus_tag="PLM92_03105"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013277845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HPR24161.1"
                     /translation="MNNTKTKFEIKNLDLYYGDFQALKKVGIDIYEREITALIGPSGC
                     GKSTFLRTLNRMNDLIPGTRIEGQVNFDNKDIYDKDTGVIELRKKVGMVFQKPNPFPK
                     SIYDNITFGPRIHGIKEKNRLNEITERTLKEVGLWDEVKDRLNKSALSISGGQQQRLC
                     IARCLAVEPEVLLMDEPTSALDPVATSRIEALLQELAKKYTIVIVTHNMQQAGRISGY
                     TSFFLTGEIIETGLTEKIFSVPENKKTEEYISGRFG"
     gene            complement(4399..5292)
                     /gene="pstA"
                     /locus_tag="PLM92_03110"
     CDS             complement(4399..5292)
                     /gene="pstA"
                     /locus_tag="PLM92_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011917139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HPR24162.1"
                     /translation="MIKESIREKGPKRLKSLLFTRTIQIIAAITLAALLIIIGYILVK
                     GLPGITWEFLSEDPSAMGREGGIFSIIVGTLYVTVLAVLIAMPISVGAAVYFNEYASG
                     KRTVAVIRFFTEVLAGIPSIIFGLFGFAFFVVFLGLGWSCLSGGLTLTIMILPTIIRT
                     TEEALKTVSMAYREGSLALGATKWQTIVKVVLPCCRGGILTGLILGLGRAIGETAAVM
                     LTVGGSLTMPVSVMDSARTMALHLYNLASEGISEEKTYATAALLIIVILIINTAANNI
                     GKRPFKGGRHKKILLQGAKDE"
     gene            complement(5289..6146)
                     /gene="pstC"
                     /locus_tag="PLM92_03115"
     CDS             complement(5289..6146)
                     /gene="pstC"
                     /locus_tag="PLM92_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013411930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HPR24163.1"
                     /translation="MNAKEKIFEKLILISAVVSVAAVALITVFIFNSGLPAIRETGAA
                     EFITGTDWSPTNGQFGILPLIAGTLAVTVLALLIGIPTGVGCAVFLAEITGEKTARIF
                     RSAIELLAGIPSVVYGFFGLVILVPVIRNIFGGSGFSIIAGGIILAVMILPTIVSISA
                     NSISSVPAEFTEASLSLGADRRETIMKVILPAAKSGIFSSVILGMGRAIGETMAVLLI
                     TGNVAKMPEAVTDPVATLTGTIAMEMPYATPQHQQALFGIGIILFIIIVLLNGTAQLI
                     MKRFGGAGS"
     gene            complement(6148..>6490)
                     /locus_tag="PLM92_03120"
     CDS             complement(6148..>6490)
                     /locus_tag="PLM92_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HPR24164.1"
                     /translation="VLVKDDEGNETDNTTADALVQPSTGSVIKTVSSTPDSIGYVSLA
                     AVNDEVKALKVEGVEPLKDTVLDGTYKISRPFVYVTGKSISDSAKAFIDFVLSDKGQK
                     LVEENGFIPVQ"
BASE COUNT         1577 a         1628 c         1248 g         2037 t
ORIGIN      
        1 tgtttactat gagtatccca gttcttccct gtcaaaggtc gtgccgtaaa aaagatcgta
       61 aaaatcattt acgttttttt caagtgcatc gtctccaagc agttccggat acattttgtt
      121 catcatataa tctatgccaa gggcacttac ggctcccgga aactcccagg aatctcccgg
      181 ggcaggcatt tcatatacat gcttgttttt tacggctttt atctcgctcc atgtctcatt
      241 gctcagtatg tcctttgcgt tataatccgt gctttcactg cttataaata tatattccgg
      301 atcccattcg aatatctgct ctgtacctgc cgtgggccat atctccgtag cctgaaggtc
      361 agcagccaca tttttccctc cggctttttc tatcatgaat ccctggagca tactgccgtc
      421 ggcaacttta cctatggaag aacccatcat gaggacagtc ttttttttct ttattttttc
      481 tgtgagtttt ttgtcttcct tcagcttatc gttatagtag tctataaact gccgggcttt
      541 tttctctgtg ccacatactt tccctattat atccagtgca acaacgatat cttcctgggt
      601 ttccattgaa agacctattg ccaccacccc cacatctccc gccgcatcga gacctgcctt
      661 atcggaggcc ttatggaaaa ccacatccgg ggcgagttct gccagagctt caatgtctat
      721 tacccctgtt cccacagaag gcacatctgc cagagacggc tgaacctttt taagaaaatc
      781 atccaccttt cctgctcctg taatggaatc acccatgcca agactgatga ggactctggc
      841 cgggactctg taacagtatg ccacagtttt tactttttcg ggaacataga cttccctgcc
      901 tgcctgatcg acaataacaa aaccctcact gtccttctca atgacagttc cgggtttttg
      961 cgtttctgtt ttctcaccgc cacatgaaac ggcagtcagc accagtacga gtataagtaa
     1021 tacacaaaat gtttttttca ttcagatcct cttatcaaaa atccggcatc acacagactt
     1081 ttcggccgat ctcttctatg tatataagcc tgatatctgt gtcatatatt cctgaaagtc
     1141 tttcttcggt caatatttct gaggcttctc cctgggtcat cttgcctttt ctgtctatta
     1201 ttgcggtctt tcctcccagc atcagtgcat gatccgggtt gtgtgtcgtt atgactatgg
     1261 caaatccttc ggaagtcatt tttcttatca taccaaggac tctgtgctgg ttaccgaaat
     1321 ccagatgggc agtgggctca tcaaaaagga tgacctcagg ttcctggacc atggctcttg
     1381 ctataagagc ctgctgcctt tcacctccgc ttatttctat gtatgatctg tcagcaaggt
     1441 gatctatgcc catttcctta agtacttttg atgctatctt tttatcttct tctcccggac
     1501 tctcaaaagt tccctttttt ggagctctgc ccataagaac aaaatctccc actttatagt
     1561 caaaggccgg tgtatgaagc tgagggacat aagccgcttt gccggctatt tctttggctg
     1621 tcatttcctt tgtttttctg ccgcacagtt ctatacttcc atgatccggt ttgagcagtc
     1681 ccgccatgca gttgagcaat gtggtttttc ctgctccgtt ggctcccagc acagaaagta
     1741 tctccccctt ctttaataca agagagatcc ggtccagtat ctgcctgctt cctttgggat
     1801 atgtaaatga aacatctttt gcttcatata ttattttttc atctgtattt ttttgttcag
     1861 tcatctcagt ctgctccttt gcctgtaaag aagccatgcg taaaacggtg cgcctattat
     1921 tccggtaagt atgcttattg gcatctctgc cggtcctatg agtcttgtgg ctgtgtcaac
     1981 cgtcagaagg aatatgcctc ccagcaaaca tgcggaaggc aaaaggactc tgtttgccgg
     2041 tccgactatc atcctgcaaa gatgagggat cacaaggcct acccatccga tggttccggc
     2101 tatgcatact gcacttgccg taagcaccgt ggcgcaaact ataaatactc cccgcattac
     2161 cccaacggat actcccagca tttgagcttc attttcaccc agagaaagta tgtccaccca
     2221 ccatgacatt gccaggatca aaaatgcggc ggcaactgca aagggcagaa cataaagaag
     2281 attattcatg tccacatatg caaaacttcc catctgccag taagtgatag atgccagctg
     2341 agtctcggga tctgctatat attttatgaa tcccaaaaca gaagacatgg ctccgcctac
     2401 tattatgcct gccaggacca gcattatatt tgaattgctt ttcagagcct ttggtatgag
     2461 aagggtaaga gttactgctg atatacctcc caaaaatgca aaggcctgta tgaaaatcgc
     2521 tgaaaaagac atgagtatag caactgctgc tcccacacat gccccggtgg atatgccaag
     2581 aaattcaggt gaaacaaggg gatttttgaa aactccctgg tatactgctc ccgccagaga
     2641 aagggcagcg cccacaaaaa cagccgccag aactcttggg agcctgatac ccaaaagcat
     2701 gttaatgtcc atctccgaca tttcaccgaa tatgttcaga gaatatttac ctatgccaag
     2761 acaaaatgcc ataaggcata aaagtactgc aaaaaaaatt acgttagtta ctactccatt
     2821 tcgcgtgtcc atatcattaa cattatacac gaaataaaaa ttttgaatag cataataata
     2881 aaagagtccg ctatgtgcga actctcccga tcaatcttct gttttcccgt tttttctatg
     2941 tgagatcctc ttctttgtca cagatccatc ctgtatctcc cttccatgga aaaaacagtt
     3001 tcctcgcaaa tattggtggc ataatcggcg atcctctcca ggtgcgccgc aacgaaggca
     3061 aaggagatgg ctgttctggc attttccgtg tttttttcgg ctatatccag ctgttcttca
     3121 tatatgtacc ggaacagctc gtccacctct tcatcgctct tccagacctt tcttgccatc
     3181 ctcagatcct tttccacaaa agctcttatg gcattgctta ccatccttgc tgccagatct
     3241 gccattctgg gtatgtctat aagttccttg tagtactccc ggttttccaa acgcaaagta
     3301 tattttgcca gatcttcgca atgatccccg atgcgctcca gatctcttat cattttcatt
     3361 atggaaatac agtacctgag atctcccgcc agcggagatt gggtggctaa aaatttgaag
     3421 cagtcggaat ttatttcatc ctccatgtca tctattttct cgtccccgtc tattattttt
     3481 cttgccaact caacatcctt ttcctttagc gattttattg aacttcgtat ggtttcttct
     3541 atcaactctg ccatttctat gactttctta ttgatcgctt ttaattcttc atccattttc
     3601 ggtcttgttg ccattttgtt ttctcctttc tatctttttt atcagccaaa acgtcctgat
     3661 atatattctt ctgtcttttt gttttccgga acagaaaata ttttttcagt gagtccggtc
     3721 tctattatct ctcccgtcag gaaaaaagat gtgtatccgg atatccgtcc ggcctgctgc
     3781 atgttatggg taactattac tatggtatat ttttttgcca gttcctgcag aagcgcttct
     3841 atccttgatg ttgccaccgg atcaagggct gaagtgggtt catccattag aagcacttcc
     3901 ggctccaccg ccaggcacct ggctatacac agtctttgct gctgtccccc cgaaatactc
     3961 agggcagact tgttcagtct gtcttttacc tcatcccaaa gacctacttc cttaagggtc
     4021 ctttctgtta tttcattcag ccggtttttt tcctttatgc catgtatcct gggcccgaag
     4081 gttatattgt catatatgct tttgggaaag ggattgggct tctgaaaaac cattcccacc
     4141 tttttgcgca gttctatcac accggtatct ttatcataaa tatctttatt atcgaaattg
     4201 acctgtcctt cgatcctcgt gcccggtata agatcattca ttctgttaag tgttcttaaa
     4261 aatgtagatt taccgcaacc ggaaggacct ataagtgctg ttatttccct ttcataaatg
     4321 tcgatcccaa cttttttcag ggcctgaaaa tctccgtaat agaggtcgag attttttatc
     4381 tcaaattttg tttttgtatt attcatcttt tgctccttga agtaatattt ttttgtgtct
     4441 tcccccttta aaaggtcttt tacctatatt gtttgcagcg gtatttatta taagtataac
     4501 tatgatgagc agtgctgcgg tggcgtaagt tttttcttcg cttattcctt cagatgccag
     4561 attatatagg tgaagggcca tggttcttgc ggaatccata acagacaccg gcattgtaag
     4621 ggacccgcct acagtaagca ttactgcggc tgtttctccg atggctctgc caagaccaag
     4681 tatcaggcct gtcagtatgc cccctctgca acagggcagt accaccttta caatggtctg
     4741 ccactttgtg gccccgagag caagacttcc ttctctatat gccatagata cggttttcag
     4801 tgcttcttca gttgttctga ttatggttgg caggatcatt atggtcagtg tcagtccacc
     4861 cgataaacag gaccatccaa gacccagaaa aaccacaaag aatgcaaatc cgaaaagtcc
     4921 gaatatgatg gagggtatgc ctgccaaaac ctcagtaaaa aaccttatta ccgccacagt
     4981 cctttttcct gatgcatatt cgttgaagta aacggctgcc cccacactta tgggcattgc
     5041 tatcaaaaca gccaggacgg tcacataaag tgtcccaact attattgaga aaatgcctcc
     5101 ttctctgccc atggcactgg gatcttccga aagaaattcc catgttatgc cgggcagtcc
     5161 tttcacaagt atatatccta ttatgatgag cagtgcagcc agggtaatag ccgctattat
     5221 ctggatagtt ctggtgaaaa gaagactttt cagccttttg ggtccttttt ctcttatgct
     5281 ttccttgatc atgatcctgc acccccaaac cttttcatta tcagctgagc tgttccgttg
     5341 agcagtacta ttatgataaa aagtattata cctatgccaa aaagagcctg ctgatgttgc
     5401 ggtgtcgcat atggcatttc catggctatg gtcccggtga gagtagccac cggatcagtc
     5461 acggcttccg gcatctttgc cacgttgcct gttatgagca gcacagccat tgtttctcct
     5521 atcgctcttc ccatccccag tatgacagat gaaaatatgc ctgactttgc agccggaagt
     5581 attactttca ttatagtttc acgccggtcg gcacccagag aaagggatgc ttctgtgaac
     5641 tctgccggaa ctgaagatat ggaatttgcg gatatactga ctattgtggg aagtatcatg
     5701 actgccagta ttattcctcc ggctattatg ctgaacccgc ttcccccgaa aatatttctt
     5761 atgaccggta ccaggatgac cagtccgaaa aatccgtata ccaccgaagg tatgcctgcc
     5821 agaagttcta tggctgaacg aaatatccgc gcagtttttt ctcctgttat ttccgccagg
     5881 aatacggcac aacccacacc ggtgggtatc cctatcaaaa gagccagtac cgtaacggcc
     5941 agagtccccg caataagggg gagtatgccg aactggccgt ttgtaggact ccagtcagtt
     6001 cctgttataa attcagcggc tcctgtttct cttatggccg gcagaccgga attgaatata
     6061 aatacagtta tcaaagccac cgctgccaca gagacaacgg cgcttatcag tatcaatttt
     6121 tcaaatattt tttcttttgc attcatatta ctgtaccggt atgaacccgt tttcttctac
     6181 aagcttctgt cctttgtcgc tcaaaacaaa gtctatgaaa gcttttgccg aatcacttat
     6241 acttttgccg gtaacgtata cgaagggtct tgagatcttg taggtcccgt ccagcacagt
     6301 atcctttaag ggctcaacgc cttccacctt cagcgccttg acttcatcgt ttactgcagc
     6361 cagtgaaaca tatcctatgg aatccggtgt tgaagaaaca gtttttataa ctgatcccgt
     6421 tgagggctgc accagtgcgt cagcagttgt gttgtctgtt tcgttgcctt catcatcctt
     6481 aacaagtaca
//
LOCUS       DAONHK010000053         6488 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_9219_length_6488_cov_4.250427, whole genome shotgun sequence.
ACCESSION   DAONHK010000053 DAONHK010000000
VERSION     DAONHK010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6488
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9219_length_6488_cov_4.250427"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..490)
                     /gene="dhaS"
                     /locus_tag="PLM92_03125"
     CDS             complement(<1..490)
                     /gene="dhaS"
                     /locus_tag="PLM92_03125"
                     /inference="COORDINATES: protein motif:HMM:TIGR02366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase transcriptional
                     activator DhaS"
                     /protein_id="HPR24165.1"
                     /translation="MSKGDITKREIAKAFKKLMEEKAFEHITISDIMNECGLNRLTFY
                     HHFQDKYALLNWIYYNEIISCFTEKLNVVNWVSNLKKALTVIKENQIYYENAFSQDND
                     EFGRYVFEVTEELFFDAIKKTQIAESNMVEEEDMAFVAKYFAYGVSGMITEWVTKGMK
                     ESP"
     gene            complement(532..1962)
                     /gene="glnA"
                     /locus_tag="PLM92_03130"
     CDS             complement(532..1962)
                     /gene="glnA"
                     /locus_tag="PLM92_03130"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HPR24166.1"
                     /translation="MFNNLAEAKKFIEENGIEMVDFMMIDLIGRWRHLTVPSDRFDEE
                     TLRSGIGFDGSNYGFAPVEKSDMVFIPDLTTAYVDQFMKIPTLAMIGDVFVIKSPDNL
                     PFSQYPRNVSKRAEAYLKSTGIADELRIGPEFEFHVFDHVSYSADPESSGFRVDVEQA
                     EWNKGFAGSNLGYKVNNKEGYHIASPKDRLYDFRSRVCMQMEKQNIAVKYHHHEVGGP
                     GQIEIEIEFGGITEMADKTMITKYIIKNQAIAEGKTATFMPKPIYDEAGNGMHIHMHL
                     FKEGKPLFYDENGYSQLSDTALYFIGGVLKHIAALCAFTDPSTNSYKRLVPGYEAPVT
                     VGFATANRSSVIRIPAYAKDPDKKRFEIRNPDGTCNPYYAYAAILMAGIDGIKKKTDP
                     VKEGFGPYDFNLYDLKDGDKKKIKSLPQFLEVALEALKNDHDFLTEGDVFPKELIDIW
                     LEKKNGDVFRQTQMPTPMEFDMYYDL"
     gene            2143..3624
                     /locus_tag="PLM92_03135"
     CDS             2143..3624
                     /locus_tag="PLM92_03135"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HPR24167.1"
                     /translation="MIRSIVQSVYAHAKSTPEKLCMADGKKEITYESAWKSIYGFSKE
                     LLDRGLSKKQCVVAECTQDVDFLVTALSVHLAGGIFVPVEKNAAAGQILEIIKQTGAG
                     FYIGRQKPDADLFFMPSGNACSFSVIDFPDISEENFPMGEETSEILFSTGTTGKSKGI
                     ELSFSNDIALAENVMHGVEMKKDNIELIPMPLSHSHGLRRCFANFLNGSSVVVCNGVI
                     LIKQFFDLIDKYRVTAIDLSPSILCIIFKLSKDTIGNYSNQLDYIQLGSAPLIEEDKD
                     RLVKLLPKTRLYNFYGTTEAGCSCIMNFAKMPDKYGCIGRPAKNASFIFVDENRNPFE
                     ATEEDPGFLASRGAMNMKGYYNDPELTAQTMSGGYIYTNDLCYRGEGGLIYYIGRKDD
                     VINCGGIKIAPDEIESVIMKYPAIKDCACIPIPDELKGQAPKIYLCVDPSCSFDSNDF
                     QDYIKKNLDGNKLPKTVEHIEEIPRTYNGKLQRKKLIVMNEKK"
     gene            complement(3680..4462)
                     /locus_tag="PLM92_03140"
     CDS             complement(3680..4462)
                     /locus_tag="PLM92_03140"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005359006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPR24168.1"
                     /translation="MSSEIKKTPVLRISGLTKNYNDAPDSRNIIESLDLDVPDGEFLC
                     ILGPSGCGKTTLIRCIAGFEDYNGTIEVDKKKVSKPAIDRIMVFQDFNQLFPWKTVEK
                     NIQYPLKKNGTTDKEKLKAIAKEHLDLVGLTEYKDFYPHQLSGGMKQRVAIAKGLALK
                     PKIILMDEPFASLDAMTRNRLQAELLNIKQKENVTVIFITHNIQEAIVLGNRILLMSK
                     DGKIKLDVKNEIQKPVTPASEGYGDMWKLFNEALQSELDQKA"
     gene            complement(4459..5226)
                     /locus_tag="PLM92_03145"
     CDS             complement(4459..5226)
                     /locus_tag="PLM92_03145"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPR24169.1"
                     /translation="MKAATRQIIFVICFIIAWQIIYSMKAFPEILFPSVTSIFEALKE
                     GFVADDLMSMVLYSLSLILKGMLIGIVLAFIFSSLAVVNRVFYDIYNLIVSIMDLIPG
                     VALLPLAILWFGIGEITIIFIMIHSILWPMSRSIIDGFSSVSPIYIESGENIGLKGIG
                     LVLGVYLPAAFTHVLTGLRVGWARAWRGLISVEMIFGTTGSGAGIGWYIYMRRAHIDI
                     AGVLATLLVIILIGVIVEYAIFRTVEKNTVRKWGMVR"
     gene            complement(5232..6215)
                     /locus_tag="PLM92_03150"
     CDS             complement(5232..6215)
                     /locus_tag="PLM92_03150"
                     /inference="COORDINATES: protein motif:HMM:NF024772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPR24170.1"
                     /translation="MKKTRKFFAVLLSAAMVGAFSIVITGCGEEETAESASLSIVEQY
                     GMAYAPLKVMQEQKLIEKNYNGDVEIEWATLNSGAAITESFASGDVDVGAMGVGPAIT
                     GALSGVGFKVCSNMSAQPHKIMTNDDSIETVADIGTEDKIALVNEGSFQHIVLAMQCK
                     ETLGEAHALDENISAMAHPDGMSALIAGSVSCQLTTSPFTFKEEAEGMSEVGNLADVW
                     PEGNSFIVLCASEKLHDDNPELYDAVVAACAEAINWINDNPQEAAELLCEDEGVDAET
                     MLSWLEDPACVYNTEVKGIMDFHDFMAENDFLEVDPAESISDLVFDNVKGN"
     gene            complement(6253..>6488)
                     /locus_tag="PLM92_03155"
     CDS             complement(6253..>6488)
                     /locus_tag="PLM92_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPR24171.1"
                     /translation="TEQTTTSFALTIEGAGISFMTESAVRYGNFKKHPAYYFTEKDIC
                     SRKTYVAYAANRGLSRAGEEFIKILKECLAALQ"
BASE COUNT         1781 a         1515 c         1224 g         1968 t
ORIGIN      
        1 caggactttc tttcataccc tttgtgaccc actctgttat catgccggat accccatacg
       61 caaaatattt ggcaacaaag gccatatcct cttcctcgac catattgctt tccgctatct
      121 gtgttttttt aatggcatca aagaagagtt cctctgttac ttcaaaaaca taccttccga
      181 attcatcgtt atcctgactg aaagcatttt cataatatat ctggttttcc ttgataacgg
      241 taagcgcctt tttcagattg ctcacccaat tgaccacatt cagtttctct gtgaaacagg
      301 atatgatctc attataatat atccagttca agagagcata cttatcctga aaatgatgat
      361 aaaaagtaag cctgttcagc ccgcattcgt tcattatgtc cgatatggta atgtgttcaa
      421 aggctttctc ttccataagt tttttaaaag cctttgctat ttcccttttt gtaatatcac
      481 cctttgacat tctatcaccg cagtaccttt ctgcctttta caccgccgac cttacaggtc
      541 gtaatacatg tcgaattcca ttggtgtagg catctgtgtc tgtctgaaca catctccgtt
      601 tttcttttcc agccatatat ctataagttc tttggggaaa acgtcaccct cagtaagaaa
      661 atcatgatca tttttgagtg cttccaatgc cacttcaagg aattggggaa gtgactttat
      721 tttctttttg tctccgtctt ttaagtcgta aagattaaaa tcgtaagggc caaacccttc
      781 ttttaccgga tccgttttct ttttgattcc gtctatgcct gccatcagga tagccgcata
      841 tgcataatag gggttgcatg ttccgtcggg atttcttatt tcaaatctct ttttgtcagg
      901 atcttttgca taggccggta tccttattac tgagcttctg ttggcagttg caaatcccac
      961 tgtaacggga gcttcataac cgggcaccag cctcttgtat gaattggtgc tgggatcggt
     1021 aaaggcgcac agtgctgcta tatgtttaag gactcctcct ataaaataca gtgccgtgtc
     1081 cgaaagctgg gaatatccgt tttcatcata gaacaagggc tttccttcct taaaaaggtg
     1141 catgtggata tgcatgccgt ttcccgcctc atcataaatc ggcttaggca taaaagtggc
     1201 tgtcttgcct tcagcaatgg cctgattctt tattatatat tttgttatca ttgtcttgtc
     1261 ggccatttcc gtgatgcctc cgaactctat ttctatttct atctgtccgg gcccgccaac
     1321 ttcatgatga tgatacttta ctgctatgtt ctgcttctcc atctgcatac atactctgct
     1381 cctgaaatca tacagtctgt cctttggaga tgctatatga taaccttctt tattgttgac
     1441 tttataaccc aggtttgatc cggcaaaccc cttgttccat tcggcctgtt ccacatcaac
     1501 tctgaaaccc gaactttcgg gatccgcaga ataacttaca tgatcgaaaa catgaaattc
     1561 gaattcgggg ccgattctca attcatcggc tatccctgtg cttttaagat atgcttccgc
     1621 acgcttcgac acatttctgg gatactgtga aaaaggaaga ttgtcaggac tcttaataac
     1681 aaacacatct cctatcatcg ccagagtagg tattttcatg aactgatcca catatgccgt
     1741 ggtaagatcc ggtatgaata ccatatcgct tttttcaaca ggggcgaatc cgtaattgct
     1801 gccgtcaaat cctatgccgc ttcttaaggt ctcttcatca aaacggtcgg aaggtacggt
     1861 cagatgtctc catctgccta taaggtcaat catcataaaa tcaaccatct ctataccgtt
     1921 ttcttcaata aattttttcg cctctgctaa attgttaaac atactgaaaa tctccttttc
     1981 acttattcaa atacattttt cagtatagct ttcacatttt gccttgtcaa acaaaataac
     2041 acctgtatat cacaattatt ctctgtacaa aacaaaagaa tatgataaaa ttatctggcg
     2101 gaaacaactt gcaatataat aatatatgag gtgagcgtta aaatgatcag atccatagta
     2161 caatctgttt atgcccatgc aaaatcaaca cccgaaaaat tatgcatggc tgacggcaaa
     2221 aaagaaataa catatgaaag cgcatggaaa agcatttacg gtttttccaa agagcttctc
     2281 gaccgtggtc tttcaaagaa gcaatgcgtg gtggctgaat gcactcagga tgtagacttt
     2341 cttgtcacag ctctttctgt tcatctggcc ggagggattt ttgttccggt ggaaaaaaat
     2401 gccgctgcgg gacaaatact tgagataata aaacaaaccg gtgccggatt ttatatcggc
     2461 cgtcaaaaac ccgacgcaga cctttttttc atgccctcag gaaatgcctg cagtttttcg
     2521 gtcattgatt ttccggatat ttcagaagag aactttccca tgggagaaga aacttccgaa
     2581 atcctttttt ccaccggtac cacaggaaaa tcaaagggaa tcgaactctc cttcagcaat
     2641 gacatcgctc tggcggaaaa tgtaatgcac ggggtcgaaa tgaagaaaga caacatcgaa
     2701 cttatcccga tgcccctcag tcattcccac ggactcagac gctgctttgc aaattttcta
     2761 aacggcagca gtgtggttgt ctgtaacgga gtgatcctta ttaaacagtt ttttgatctt
     2821 atagataaat accgcgtaac ggctatcgat ctgtccccca gcatactttg catcatattc
     2881 aaactttcca aggataccat aggcaattac agtaatcagc tggactatat acaacttggc
     2941 tctgcacctc ttatagagga agacaaggat cgccttgtaa aacttctgcc aaaaaccagg
     3001 ctatacaatt tttacggtac caccgaggcg ggatgctcat gcataatgaa tttcgcaaag
     3061 atgccggata aatacggatg tataggcaga ccggcaaaaa acgcttcatt catttttgtg
     3121 gatgaaaaca gaaatccatt tgaggccaca gaagaagatc cgggttttct ggccagcaga
     3181 ggagcaatga atatgaaggg ttattataac gatcccgaac ttactgcaca aaccatgtcc
     3241 ggaggctata tatatacaaa cgatctttgt tacaggggcg aaggcggact aatatattat
     3301 atcggccgaa aagacgatgt tataaactgc ggaggcataa agatagctcc ggacgagata
     3361 gaatccgtaa taatgaaata cccggcaata aaagactgtg catgtatacc gataccggat
     3421 gaactcaaag gacaggctcc aaagatatat ctttgtgttg atccttcctg cagctttgac
     3481 agtaatgact ttcaggatta tatcaaaaaa aatctggatg gcaacaagct ccccaaaacc
     3541 gtagagcaca tagaagagat acctcgtacc tacaacggga aactgcaacg caaaaagctc
     3601 atagtaatga atgaaaaaaa atagaaaaaa cccccgcctt ttcggcgggg gctttttgat
     3661 acaagcagtt ttaatatttt tatgcttttt gatccagctc agactgcaat gcctcgttga
     3721 aaagtttcca catatctcca tatccttccg aagcaggtgt tacaggcttt tgtatttcat
     3781 ttttcacatc aagcttgatt tttccgtctt tcgacatcag caggatacga ttacccagga
     3841 caatagcttc ctgaatatta tgtgttatga aaataacagt aacgttttct ttctgcttga
     3901 tattcagaag ctccgcctgc aatcggtttc ttgtcatcgc atcaagactg gcaaaaggct
     3961 catccattaa tattatcttt ggttttaatg ccagaccctt tgcgatagcc actctctgtt
     4021 tcatgccgcc ggaaagctga tggggataaa aatccttata ttcggtaagc cccacaagat
     4081 ccagatgctc tttagctatg gctttcagtt tttctttgtc ggtagtcccg ttctttttca
     4141 aaggatactg aatgtttttt tcaactgttt tccatggaaa aagctgattg aaatcctgaa
     4201 aaaccattat cctgtctatt gccggtttgc tgaccttttt tttgtccact tctattgtcc
     4261 cgttgtaatc ttcaaacccg gcgatacacc ttataagggt cgttttcccg cagccggaag
     4321 ggccaagtat acaaaggaat tccccatccg gtacatcaag atcaagactt tctattatgt
     4381 tcctggaatc gggggcatcg ttgtagtttt ttgtcagtcc ggagatcctg agcaccggag
     4441 tttttttgat ttcgctgctc atcttaccat cccccatttc cttactgtgt tcttttcaac
     4501 ggttcggaat atggcgtatt caacaatgac gccaataagt attatgacca gcagtgttgc
     4561 cagaacaccg gcgatatcaa tgtgggcacg cctcatgtat atgtaccagc ctatgcccgc
     4621 tcccgagccg gtggtgccga atatcatctc tacacttata agtccgcgcc atgctctggc
     4681 ccatccgact ctaagtcccg tgagaacatg agtgaatgct gcaggaagat atacgcccaa
     4741 aaccagcccg atccctttta gtccaatatt ctcgccgctt tcgatatata tgggcgatac
     4801 cgaactgaag ccgtctatta tgcttcttga catgggccac aatatggaat ggatcataat
     4861 aaatattatt gttatttctc cgataccgaa ccagagtata gcaagaggca gaagcgccac
     4921 acccgggata aggtccatga tggatactat caaattgtaa atgtcataaa aaactctgtt
     4981 gacaacggcc aggctggaga atataaatgc cagtactatc cctataagca ttcctttgag
     5041 tatcaacgac aaggaataca aaaccattga cataagatca tctgccacga atccttcttt
     5101 taatgcttca aatatggagg ttactgaagg aaaaagtatc tccggaaatg ctttcattga
     5161 ataaattatc tgccatgcaa tgataaagca tatcacaaat attatttgtc ttgttgctgc
     5221 tttcatttat gttaattccc ttttacgtta tcaaatacca aatcacttat gctttccgcg
     5281 ggatccactt ccagaaaatc attctctgcc ataaaatcgt ggaaatccat aatgcccttt
     5341 acctctgtgt tgtaaacaca tgcgggatcc tcaagccagg aaagcatggt ttcggcatct
     5401 acaccctcgt cttcacaaag aagttctgcc gcttcctgcg ggttatcatt gatccagttt
     5461 atagcctccg cacaggcggc cacaacggca tcatacaact ccggattatc atcatgaagt
     5521 ttttcgcttg cacagagaac gataaatgaa tttccttccg gccatacatc tgccaggttt
     5581 cccacttcgc tcattccttc tgcttcttct ttgaaagtga atggagatgt tgtcagctgg
     5641 caagaaacgc tgcctgcgat aagggcggac atcccatccg gatgtgccat agcacttatg
     5701 ttttcatcca gtgcatgtgc ttcacccaga gtttctttac actgcatcgc cagtactata
     5761 tgctggaaac ttccctcgtt tacaagggcg atcttatcct cggtaccgat atccgccaca
     5821 gtttctatac tgtcatcgtt tgtcattatt ttatgaggct gagcggacat gttggagcat
     5881 actttaaagc cgacccccga aagagccccg gttatggcag gtccaacacc cattgcaccg
     5941 acatctacat ctcctgaggc aaacgactct gtgatcgccg cgccggaatt aagtgtggcc
     6001 cattctatct ccacatctcc gttataattc ttttcgataa gtttctgttc ctgcattact
     6061 ttcaacggag catatgccat accatactgc tcaacgatag aaagcgatgc actctctgct
     6121 gtttcttctt caccgcaacc agttattacg atggaaaaag cccccaccat agctgccgaa
     6181 agaagcacag caaaaaattt tctcgttttt ttcatgattg tttctccttt ctgataattg
     6241 ttttgaaata aattattgca gcgcagcaag gcattcttta agtattttta tgaattcctc
     6301 tcctgcgcgt gacaatcccc gatttgcggc atacgccaca tacgttttcc tgctgcatat
     6361 atctttttca gtgaaataat atgccggatg ttttttaaaa ttaccgtatc ttacagcaga
     6421 ttctgtcatg aatgatatgc cggcaccttc tatggtcaag gcaaaactgg tggtggtctg
     6481 ctcagtcg
//
LOCUS       DAONHK010000054         6438 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_9305_length_6438_cov_4.554285, whole genome shotgun sequence.
ACCESSION   DAONHK010000054 DAONHK010000000
VERSION     DAONHK010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6438
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9305_length_6438_cov_4.554285"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..898
                     /locus_tag="PLM92_03160"
     CDS             <1..898
                     /locus_tag="PLM92_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006001825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HPR24172.1"
                     /translation="LKAGSSVVAGTCAGRIRLMNNSKGETIRKAAPATAVEILGLTDV
                     PAAGDSFNAVSEDRLAKEIADKRKQKQREEVMAKTSKTSLEELFSQIKEGETKELPLI
                     IKGDVRGSVGAVTTSLEKLSNENVKVRIVHTGVGAITESDVMLAGTSGAIIIGFNVRP
                     AAGVAQQADREGIQIRNYRVIYDIIDDIESAMKGMLDPEFKEVVLGQVKVRETFKVPN
                     VGIIAGAYVTEGKVIRNAEIRLVRDGVVIHEGKISSLKRFKDDAKEVQQGFECGLGIE
                     NYNDLKEDDIIECFEIQEIKRD"
     gene            901..1281
                     /gene="rbfA"
                     /locus_tag="PLM92_03165"
     CDS             901..1281
                     /gene="rbfA"
                     /locus_tag="PLM92_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009223052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HPR24173.1"
                     /translation="MGKGYRQGRLGEEIKKIISTMLLKELKDPRLDGMVSVTAVDVTG
                     DGSLAKVYITVMSTLEEKKPEETQKQEVLEAMKSAKGLIRKEIGKQVRLRHTPDLIFK
                     LDGSLDYGRHIDEILDEVVKNDEE"
     gene            1268..2239
                     /locus_tag="PLM92_03170"
     CDS             1268..2239
                     /locus_tag="PLM92_03170"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HPR24174.1"
                     /translation="MMKNNSLKEIGEQLEGADSLVIFPHVLMDGDAMGSAVALCRVLR
                     GMGKEAWILLEDKIPDYLMFLDKGFCTYDKEKVAAPDICLCIDCGDTERFKKRVDVFQ
                     KGKSRICVDHHPTSDPFVEYNHINTEASATGEIVYDLILEMGWPIEKEAAEAIFTAIT
                     TDTGNFQYSNTTKRSHEITADLYDYSGDFSKISVEIYQNEDLSKFRLESKIIDTAEVF
                     AKGKGIIACVTQKMLEEAGARMEETEGMVAKLRSVKGVQIAVLIKENDTGEMKVAMRA
                     KEEGNVAKIAGKYNGGGHIKAAGCTIYDSMENVRKLMIKEVSRQFGY"
     gene            2229..3179
                     /gene="truB"
                     /locus_tag="PLM92_03175"
     CDS             2229..3179
                     /gene="truB"
                     /locus_tag="PLM92_03175"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002600703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HPR24175.1"
                     /translation="MDTDINGIINLRKSAKYTSHDCVAVIRKLFGVKKAGHTGTLDPM
                     AEGVLPVCLGKATRIIEYMDNDLKEYRCTMKLGVETDTQDVWGSILCEYPLKDITERS
                     IRDAVLSFRGITDQIPPKYSALKVKGKRLYKYAREGLEVEIKPRSINIEDIIIDDIMI
                     EADEEGKVEKAEVIFTVNCCKGTYIRTLCHDIGRKLGCGAAMSGLTRTVNGIFCIEDA
                     VSLQELESMSEMERIGKLLPMDHSLSGLEVIWLDDKRAGDFVNGREIRDFTATQNIED
                     KSKMENSKKRFRVYSGNTFLGIAGIEGNELRPEKVLCTKI"
     gene            3191..4135
                     /locus_tag="PLM92_03180"
     CDS             3191..4135
                     /locus_tag="PLM92_03180"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HPR24176.1"
                     /translation="MKIYNSLEGIDDAGKTVVALGNFDGVHKGHQALIARTVKAAEAA
                     EMKSAVFTFSNHPRNLTGKGPFVKSILYNEEKADIIRSLGIDILFSLPFTKDILNMRA
                     EAFINELLIKKIGMQEAYCGFNYRFGYKAEGSAEVLMHQGMKKGFGIHVLEPFRIDGQ
                     LVSSTMIRKCIEDGQVDKCLKYLGRYYSIGGEVVIGNKLGKKLGFPTSNLIVDESMVS
                     PPNGVYITYCTYEGKRYPGVTNVGRKPTVGEFEKNVETHIFNFDKELYGKKIKVEFLK
                     KTRPEKKFDTIKELSKQITEDCIAARAFHRAFDNEETC"
     gene            4161..5054
                     /locus_tag="PLM92_03185"
     CDS             4161..5054
                     /locus_tag="PLM92_03185"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HPR24177.1"
                     /translation="MDLKKAKELVIKAGEEITESGLIARTWGNISCRVDQESFVITAS
                     GRNYMTLTEEEVIPLGMKDMTHRGPVKPSSEKKLHRELYKLKPEANFIIHTHQDNASA
                     ISAMGFGSVSFDKEYDRIGKGIICADYALPGTNKLSKNTARAAKESSGNAVIMSHHGA
                     VCWGRTYEEAFAAAYGLEEAAAEYLARMGVPLKDPLSKYVSYKRLYLKAYLDDYAQIA
                     GPKMEILLDGKGKLILGKAEDREATEMIVQKNCKAYFAAFLSPKGKPISTIDSNFLRQ
                     KYLRQYSKLSKRDIGTGRHKK"
     gene            5051..5512
                     /locus_tag="PLM92_03190"
     CDS             5051..5512
                     /locus_tag="PLM92_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPR24178.1"
                     /translation="MNNWKYRLMRFMQGRYGGDQFNFFLIIASFLLEVTGNIFGIRII
                     YWIGVAMILFAIYRMFSRNIYKRQQENIRFMSFFGRITGRKKCGGYYENRGGQNMGGN
                     RQQRENRRPKDKTVYCYYNCPSCMQQVRVPAGKGKVRIKCPKCGNVFETHS"
     gene            5650..5916
                     /gene="rpsO"
                     /locus_tag="PLM92_03195"
     CDS             5650..5916
                     /gene="rpsO"
                     /locus_tag="PLM92_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013290756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HPR24179.1"
                     /translation="MIDVKKKAEIIKEYQLKEGDTGSPEVQIAVLTYRINDLNEHLKV
                     HKKDHHSRRGLLKMVGQRRNLLNYLKEKDVNRYRDLIARLGLRK"
     gene            6085..>6438
                     /locus_tag="PLM92_03200"
     CDS             6085..>6438
                     /locus_tag="PLM92_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006000391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HPR24180.1"
                     /translation="MRFEDYKTFRTAIGGKLMEMEIGKVCEQANGQVMVRYGDTVVNV
                     TAVMSKEPRPDIDFFPLSCDYEEKMYAAGKIPGGFIKREGRPSEKAVLNSRLMDRPLR
                     PLFPKGFFNDVQVVAT"
BASE COUNT         2273 a         1121 c         1616 g         1428 t
ORIGIN      
        1 ccttaaggcg ggaagcagcg tggttgcagg cacatgcgca ggtcgtataa ggctaatgaa
       61 caactcaaag ggagagacaa taagaaaagc ggctccggcc acagctgttg agatactcgg
      121 tctcactgat gttcctgctg ccggcgattc cttcaatgca gtaagcgaag accggttagc
      181 aaaagagatc gcagataaaa ggaagcagaa acagagagaa gaagtaatgg ctaagacttc
      241 caaaacaagt cttgaggaac ttttcagcca aataaaagaa ggggaaacaa aagaactccc
      301 acttattatc aaaggagatg taagaggatc ggttggtgct gtcaccacat cactggaaaa
      361 actcagtaat gagaacgtga aggtcaggat agtccatacg ggagttggtg caataaccga
      421 atcagatgtc atgctggcgg gaacttccgg ggcgataata ataggattca atgtaagacc
      481 ggctgcaggg gttgctcagc aggctgacag agaaggcata cagataagaa attacagggt
      541 catatacgat ataatagatg atatcgaaag tgccatgaag ggtatgctgg atccggagtt
      601 caaggaagtc gtgcttggcc aggtaaaagt aagggagact ttcaaagttc ccaatgtggg
      661 aataattgcc ggagcatatg ttacggaagg caaggtaata agaaatgcag agatacgtct
      721 tgtcagggac ggagtagtaa tacacgaagg taaaatatca tccctgaaaa gattcaagga
      781 tgacgcaaaa gaagtgcagc agggatttga atgcggactt ggaattgaaa actacaatga
      841 ccttaaggaa gacgatatca ttgaatgctt tgagattcag gagatcaaga gggattaaaa
      901 atgggaaaag gatatagaca gggaagactc ggtgaagaaa taaaaaaaat aataagcacc
      961 atgcttctta aggaactcaa agatcccaga ctcgatggca tggtcagcgt tactgctgtt
     1021 gatgttaccg gtgacggaag tcttgcaaag gtatatatca ccgttatgag tacacttgaa
     1081 gaaaaaaagc ccgaagagac acaaaaacag gaagtgctgg aagctatgaa aagtgcaaaa
     1141 ggcctgatac gcaaagaaat aggcaaacag gtcaggctga gacatacacc ggatctcata
     1201 ttcaaactgg atggatcact ggattacggc aggcatattg atgagatcct tgatgaagtg
     1261 gtgaaaaatg atgaagaata acagtctgaa agaaatcggc gaacaacttg aaggcgcgga
     1321 ttctttagtc atattccccc atgttttgat ggatggcgat gcaatgggat cggctgttgc
     1381 tctttgccgt gtccttcgcg ggatgggcaa ggaagcatgg atactgctgg aggacaagat
     1441 acctgattat ctaatgtttc tggacaaggg gttttgcact tacgacaaag aaaaagtagc
     1501 tgctccggat atctgtcttt gtattgactg cggggataca gaacgtttca aaaaaagagt
     1561 tgatgttttt caaaaaggca aaagcaggat atgcgtggat catcatccca cttccgatcc
     1621 ttttgttgag tacaatcata taaatacgga ggcttcagcc acaggagaaa tagtttacga
     1681 tctcatactt gaaatgggct ggcctataga aaaagaagca gcggaagcga tttttacggc
     1741 aataaccacc gacacaggga atttccagta ttccaatacc acaaaaagaa gccatgaaat
     1801 aactgcagac ctatatgatt attccggaga tttttccaaa atcagtgtgg agatatatca
     1861 gaatgaagat ctttcaaagt tcagacttga aagcaagata atcgatacgg cggaggtctt
     1921 tgcaaaagga aaaggaataa tagcatgcgt gactcaaaaa atgcttgaag aagccggagc
     1981 acgaatggaa gaaaccgaag gaatggtagc taagcttaga agtgtgaaag gcgttcagat
     2041 agccgtgctg ataaaagaaa atgacaccgg agaaatgaag gtggccatga gggcaaaaga
     2101 agaaggcaat gtggcaaaga tagcagggaa atataacgga ggcggccata taaaggcggc
     2161 cggatgtaca atatatgatt cgatggaaaa tgtcagaaag cttatgataa aggaggtatc
     2221 gaggcagttt ggatactgac ataaatggta taataaattt gaggaagtca gcaaaataca
     2281 cttctcatga ttgtgtggct gtaataagaa aacttttcgg agtaaaaaaa gcagggcata
     2341 ctgggactct tgatccaatg gcagaaggtg tcctgccggt atgtctgggc aaagccacca
     2401 ggataataga atatatggat aatgacttga aagagtacag gtgcactatg aaactgggag
     2461 tggagacgga cacccaggat gtgtggggca gtatactgtg cgaatacccc ttgaaggata
     2521 taacggagcg cagtatcaga gatgctgtgc tttctttcag gggaataaca gaccagatac
     2581 ctccaaaata ttccgcattg aaagtaaagg gaaaaaggct ttataaatat gcaagggaag
     2641 gtcttgaagt agaaataaag cccagaagta taaatataga agatattata attgatgata
     2701 taatgataga agcggatgaa gaagggaaag tggaaaaagc agaagttatc tttacagtaa
     2761 actgttgcaa gggcacatat ataagaacac tctgccatga cataggcaga aagctcggtt
     2821 gcggagcagc gatgagcgga cttacaagaa cagtaaacgg catattctgc atcgaggatg
     2881 cagtaagcct tcaagaactt gagagcatgt cagaaatgga acggataggt aaacttctgc
     2941 ctatggacca ttctctttcc ggacttgaag tcatatggct ggatgataaa agagcaggag
     3001 attttgttaa tggcagagaa ataagagatt ttactgccac acaaaatatt gaagataaga
     3061 gcaaaatgga aaattcgaaa aagcggttca gagtatattc cggaaacacc tttctgggca
     3121 ttgccggcat tgagggtaat gagttgaggc ccgaaaaagt cctttgtaca aaaatataaa
     3181 aggagagaac atgaagatat ataattccct ggaaggaata gatgatgcag gtaaaacagt
     3241 ggtagcactt ggcaactttg acggagtgca taaaggacac caggcactta ttgcgcggac
     3301 cgtaaaagcc gctgaagccg cagaaatgaa aagcgccgta ttcacttttt ccaatcatcc
     3361 gagaaatctg acaggcaaag gcccttttgt caagagcata ctatataacg aggagaaagc
     3421 cgatataata agaagtcttg gcatcgacat tcttttttcg ctgcctttca caaaagatat
     3481 actgaacatg agagcagaag catttataaa tgaactcctt ataaaaaaaa tcggcatgca
     3541 ggaagcctac tgcggattca attacagatt cggatacaag gcggaaggaa gcgccgaagt
     3601 gcttatgcac caaggcatga aaaaaggatt cggcatccat gtgcttgaac catttcgtat
     3661 agacgggcag ctggtaagtt ccaccatgat aagaaagtgt atagaagacg gacaggtgga
     3721 caagtgctta aaatatctcg gcaggtatta cagtatagga ggagaagtgg tcatcggtaa
     3781 caaactgggg aaaaaactcg gctttcccac ctccaacctt atagtagatg aaagtatggt
     3841 ctcccctccc aatggtgtgt atataacata ctgcacctat gagggcaaaa gatatccggg
     3901 agttacaaat gtgggaagga aacccacagt gggagaattt gaaaaaaatg tagagacaca
     3961 tatatttaat ttcgataaag aattatacgg taaaaaaatc aaagttgaat tcctgaaaaa
     4021 aactagaccg gaaaaaaaat ttgatactat caaagaactt tcgaaacaga taacagaaga
     4081 ctgtatagca gcaagagcat ttcacagagc ttttgataac gaagaaacat gttgatttaa
     4141 gagtcgggaa aaggaaaaaa atggacctca aaaaggcaaa ggagctggtc ataaaggccg
     4201 gagaagagat aaccgaaagc ggtcttatag ccagaacatg gggtaacata agctgcagag
     4261 ttgaccaaga gagttttgtc ataaccgcca gcggacgcaa ctatatgacc cttacagagg
     4321 aagaggtcat accgctggga atgaaagata tgactcacag gggacctgtc aaaccctcct
     4381 ccgaaaaaaa acttcacagg gaactataca aactgaaacc tgaagcgaac tttattatcc
     4441 atacacatca ggacaatgca tctgctattt cagccatggg attcggttct gtttcatttg
     4501 ataaagagta tgacagaata ggcaaaggta taatatgtgc cgactatgca ctgcctggca
     4561 ccaataaact ttcgaaaaac actgcgaggg cggcaaaaga gtcatcgggc aatgcagtga
     4621 taatgtccca tcatggagca gtgtgctggg gaaggacata tgaagaggct tttgcggccg
     4681 catacggact tgaggaagca gcggcagaat acctggcaag gatgggagtc cctcttaagg
     4741 atccgttatc aaaatatgta tcatacaaaa gattgtatct gaaggcatat ttagatgatt
     4801 atgcccagat agccggcccc aaaatggaaa tactgctgga cggcaagggg aaactgatac
     4861 tgggcaaagc ggaagacaga gaagctactg aaatgatagt tcaaaagaac tgtaaggcat
     4921 actttgcggc attcctttcc ccaaagggga aaccaataag taccatagac agcaattttc
     4981 tcagacaaaa atatttaagg cagtattcta aactgtccaa gagagatatt ggcacaggga
     5041 ggcataaaaa atgaataact ggaaatacag actgatgaga tttatgcagg gaagatacgg
     5101 cggcgaccag ttcaattttt tccttataat agcgtctttt ttactggaag tgacaggcaa
     5161 catatttgga ataaggataa tatactggat aggagttgca atgatcctct ttgctattta
     5221 cagaatgttt tcaagaaata tatacaaaag acagcaggaa aatatcagat tcatgtcatt
     5281 ttttgggaga atcacaggca gaaagaagtg cggcggatat tacgagaacc gtggaggtca
     5341 gaatatggga ggaaaccgac agcagcgtga aaacagaaga ccaaaggaca agacagtgta
     5401 ctgctattac aactgcccct catgcatgca gcaggtcagg gtgccggcag ggaaaggaaa
     5461 agtccgtata aaatgcccca aatgcggcaa tgtattcgaa acacattcat gatgatcccg
     5521 gagtttggct cttgcattaa catgatgttt gtgttagtat aaaccgttaa tatttttacc
     5581 ttttgctgag ttttacgaaa ctccgacgga aacttcgcag aaggcgtatg atgaaaagga
     5641 gaaaacaaaa tgatcgatgt aaaaaagaaa gctgaaataa tcaaagagta tcagctaaaa
     5701 gaaggggata caggatcccc ggaagtccag atcgccgttc tgacctacag aataaatgat
     5761 ctgaatgaac acctgaaggt ccacaaaaag gaccaccatt caagacgcgg ccttcttaaa
     5821 atggtcggac agagaaggaa tcttctcaat tacctgaagg aaaaggacgt gaacaggtac
     5881 agagatctta tagcccgtct gggattgaga aagtaatttc aagaaattta agcggagtaa
     5941 agctccgctt ttatttataa gtgcaataaa gcacaaaagt attttgaagc gtatgatcag
     6001 tatgtcattt gtatcataag gcaaaaagat cacgcgcaga aggtaatgcg cggaaagcgc
     6061 agaattagca ggaggtagtt gctaatgaga tttgaagatt ataaaacatt cagaacagcc
     6121 atcggcggaa aactgatgga gatggaaatc ggcaaggtct gtgaacaggc caacgggcag
     6181 gtaatggtca gatacgggga tacggtcgta aatgttacag cagtaatgtc aaaagaaccc
     6241 agacccgata ttgacttttt cccgctcagc tgtgactatg aagaaaaaat gtatgctgcg
     6301 gggaagatac ccggaggatt catcaaaaga gaaggaagac ccagtgaaaa ggccgttttg
     6361 aattccaggc tcatggacag accgctccga ccgcttttcc caaagggttt ctttaacgat
     6421 gtgcaggtgg tggccact
//
LOCUS       DAONHK010000055         6358 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_9466_length_6358_cov_4.516103, whole genome shotgun sequence.
ACCESSION   DAONHK010000055 DAONHK010000000
VERSION     DAONHK010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6358)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6358)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6358
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9466_length_6358_cov_4.516103"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="PLM92_03205"
     CDS             complement(<1..626)
                     /locus_tag="PLM92_03205"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HPR24181.1"
                     /translation="MKVINSTENKIYKMCIKLGSKKYRDRLGLYIIEGEKLVKEAEAA
                     GMAEMVIIKEDHPRKEEFGGSAKVFMKGSLFDKAAQTKTTQGVFAVAAKKEYDPKEFF
                     EITAGKNLIVLDRLQDPGNIGTIIRTAEAAGYGGAIIMKGSGDIYSSKAVRAAAGSIL
                     RFPVMLAEEAGSTAELLLKNGRRIISTKADAKKDFRETDLTENTALVIG"
     gene            complement(691..1803)
                     /gene="ftsZ"
                     /locus_tag="PLM92_03210"
     CDS             complement(691..1803)
                     /gene="ftsZ"
                     /locus_tag="PLM92_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HPR24182.1"
                     /translation="MLQFEMKEKSNANLIVIGVGGGGCNAVNRMIDDGMKGAEFLAIN
                     TDKQALSACKAENKLQIGEKLTKGLGAGGNPEIGQKSAEENMEDIAQFVSGAEMVFVT
                     AGMGGGTGTGAAPVVAKVAKDMGILTVGVVTKPFGFEGRKRSEHAELGIKFLKKYVDS
                     MVVVPNDKLLKLAEENTSLLTAFNMADGVLRHGVQGITDLIGDDGLINLDFADVETVM
                     RDRGVAHMGVGLGKGDDKVREAVKTAIESPLLETTVAGAKSILLNVTGGYDIGMMDVN
                     DAANQIQSVADKDAIIIFGAAVKEDMKDEMYITVIATGFENKPSNYASTVNLFKAEPP
                     ATEQKEDEPEGTEKPGEETGQKESPEKKFDIPSFLK"
     gene            complement(1898..2635)
                     /locus_tag="PLM92_03215"
     CDS             complement(1898..2635)
                     /locus_tag="PLM92_03215"
                     /inference="COORDINATES: protein motif:HMM:NF020067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HPR24183.1"
                     /translation="MTHVRKKRKKKHYFLKFLLFAALCTGLFFLLSSEYFDIQKIEVK
                     GTNNYTKQQILKMSGIKKGENIFALRARKTRENMKKNSYIYDVKIRRSLPDKVVLEIK
                     ERKEQAAVPYEDSYIMIDKEGYVLKKTKEQPKLTIIQGMTIERMKEGKKMKVKEGIVL
                     EDTLTMLVTMKKSDLFFKKIDISRVIIKAYIYDSLVCEGTPSNIMKSMKNGDLEAVLY
                     DLYQKDIKRGVINIGEDDYCSFSPAIN"
     gene            complement(2632..3750)
                     /gene="murG"
                     /locus_tag="PLM92_03220"
     CDS             complement(2632..3750)
                     /gene="murG"
                     /locus_tag="PLM92_03220"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HPR24184.1"
                     /translation="MKVIMTGGGTGGHIYPAIAIADKIKEKSPSSEIMFIGVKRGKEN
                     ELVPAAGYKIEFIKVHGLDRKNLFRNINVSREYLQARKDAGKIIKQFCPDAVIGTGGY
                     VCAPALKAAHLQGIKTYIQEQNAMPGLANRMMEKHVEKVFLGFEEAGKGFKYPDKHIV
                     TGNPVRKEFFSADKKEAKKEARKELGIKQKDFVLLAFGGSQGAGRINKSMISVIEKYN
                     GRENMQIFFATGSYYHMPVMNELGEKGIRLKENVNVISYINDMHKYLNAADLVISRSG
                     AITVSEIAVCGKPSILIPSPIVTGDHQTFNAKVLSEKGAAILMEEKNMREETLEQEIE
                     KLIKDDEALSKMAKSAEKCAPVDAAEMICHYIADREQI"
     gene            complement(3747..4877)
                     /gene="ftsW"
                     /locus_tag="PLM92_03225"
     CDS             complement(3747..4877)
                     /gene="ftsW"
                     /locus_tag="PLM92_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013150476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HPR24185.1"
                     /translation="MKKHGIQLQSGDFVLTIMTLVLVIFGVIMVFSSSYYSSINEGGT
                     PYSYLIRDIIWATAGFALMMLLTVVDYRFYKKIALPGIILSIGLLALVLTPVGTEINN
                     ASRWIDVGPVTVMPGEITKIAVIIFVAWFLSRKSNVIKSLFTGVLPLIFLAGLCGGLV
                     MMQPNMSTALIIGGIIFAMMFVAGINLIYFAGMIGAGATVAAALIISDDTGYRMARVM
                     SFLDPFSDPQGTGYQVVQSLLALGSGGLFGLGLGNSVQKTLYLPEPQNDFILAIIGEE
                     LGFVGIILLMTCYLILIWRGIRIAVAAPDRFGMLLASGITIMLGLQIIMNVAVVTSLM
                     PPTGVTLPFVSYGGNALLLFMGSMGILLNISKNENAERQDIK"
     gene            complement(4877..6241)
                     /gene="murD"
                     /locus_tag="PLM92_03230"
     CDS             complement(4877..6241)
                     /gene="murD"
                     /locus_tag="PLM92_03230"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005999670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HPR24186.1"
                     /translation="MEEIKGKKVLVVGLGKSGIAAVQALQKLGAKVYVQDVKKREELD
                     GQLLGLLESREVECYLGVTPDENEYFDLLILSPGVRPDMGFIKKAKENGAEIIGELEI
                     AYRTGNGRYVAITGTNGKTTTTALTGEIFKNAGRKTSVVGNIGVAVISKAINADDDTW
                     LVTEVSSFQLETIRDFKPEISAILNLTPDHLDRHGDINKYGTAKARIFENQQEDGYVV
                     INHDDKECMKLASGAKAKIIPFSRTEKLDLGAFVDDGKMVIVDEEGKKTEFCGTEELY
                     VPGAHNVENALAAMAVAYFAGIDSEVITNTLKEFKGVEHRIEFVGEVDGVRFVNDSKG
                     TNTDASIKAIEAMKGNVILIAGGYDKGSSYEELIRSFGDKVRFAVLMGKTAVKIKDAA
                     EKTGFTNTIIVKDMEACVREGFRLARPGDTVLLSPACASWDMYTNFEQRGEHFKDCVK
                     RLES"
BASE COUNT         1437 a         1649 c         1133 g         2139 t
ORIGIN      
        1 cctatcacca gagcggtatt ttcggtcaga tccgtttctc tgaaatcctt ttttgcgtct
       61 gcctttgtgc tgatgatcct tctgccgttt ttcaaaagca gttctgcggt actgcccgct
      121 tcttccgcta acatcacagg aaatcttaat atggaaccgg ctgcagctct tactgccttt
      181 gaagaatata tatctccgct gcccttcatt atgatcgcgc ctccgtatcc ggccgcctca
      241 gctgtcctta ttattgttcc tatattcccg ggatcctgca acctgtccag aactatcagg
      301 ttctttccgg cagttatttc aaagaactct tttgggtcat attctttttt tgccgccaca
      361 gcaaaaacgc cctgagtggt ctttgtttgg gcagctttat caaaaagact gcccttcata
      421 aagactttgg cagatccgcc gaattcttcc ttccgcggat gatcttcttt tattatgacc
      481 atttctgcca tgcctgcagc ctctgcctct ttaacgagtt tttcgccctc tattatataa
      541 agtcccagtc tgtctctgta ctttttgctt cccagcttta tacacatctt atatattttg
      601 ttttctgttg agtttattac tttcatagtt gctcttaatc cagaaaagcc ggtataatac
      661 cggctctcct tattttatat gctcatattt ctatttaaga aatgacggga tatcgaattt
      721 tttttcggga gattcttttt gaccggtctc ttctccgggt ttttcggttc cttccggctc
      781 gtcttccttt tgttctgttg ccggcggttc cgctttgaac aggtttaccg tggaggcata
      841 atttgaaggt ttgttttcaa atccggtggc tatgacggtt atatacattt catctttcat
      901 atcttctttg actgctgccc cgaatattat tattgcatcc ttatccgcaa cgctctgtat
      961 ctggttagcg gcatcattta catccatcat gccaatgtca tatccgccgg tcacattcag
     1021 aagaatggat tttgctccgg caacggtggt ttccagaaga ggactttcga tggcggtctt
     1081 gactgcttct cttaccttgt cgtctccctt gcccaggcct acgcccatgt gagcaacacc
     1141 tctgtctctc atcaccgttt ccacatctgc aaagtcaaga tttataagtc cgtcatcgcc
     1201 tatcagatcg gttattccct ggactccgtg cctcaagact ccgtctgcca tgttgaaggc
     1261 cgtaagcagt gaggtgtttt cttcagccag tttcaacagt ttgtcattgg gcaccaccac
     1321 catagagtcc acgtattttt tgaggaattt tattccaagc tcggcatgct cgcttctttt
     1381 tcgtccttca aatccgaagg gcttcgttac tacccctacg gtgagtattc ccatgtcctt
     1441 tgcaactttt gccacaaccg gtgcagcacc tgttccggta ccgcctccca tgcctgccgt
     1501 aacgaatacc atttcggccc cgctgacaaa ctgggcaatg tcttccatat tttcttctgc
     1561 ggatttttgg ccgatctcgg gattgccccc tgctccgagt ccctttgtca gtttttcgcc
     1621 tatctgcagt ttgttttcgg ctttacatgc tgaaagtgcc tgtttatccg tattgattgc
     1681 caggaattcg gcacctttca ttccatcatc gatcattctg ttaactgcat tacatccgcc
     1741 tccgccaaca cctataacaa taaggtttgc gttgcttttt tctttcattt caaactgtaa
     1801 catctgacag catcctcccg acacgaccac ttttatatat acggtattat tatagcatat
     1861 tatcgggctt ttgcacaata atttacttac gcatatttca gtttattgca ggactgaacg
     1921 agcagtaatc atcttcaccg atattgataa cgcccctttt tatgtccttt tgataaagat
     1981 cgtaaagcac cgcttccagg tctccgtttt tcatgctctt cattatattt gacggtgtcc
     2041 cttcgcacac aagactgtcg tatatatatg cctttattat tactctggat atgtctattt
     2101 ttttaaaaaa caggtcgctt tttttcatgg tcacaagcat ggtcagcgta tcttccagga
     2161 ctatcccttc tttgaccttc atttttttgc cctctttcat tctttctatg gtcattccct
     2221 gtataatggt cagcttgggt tgttcttttg tttttttcag cacatagcct tccttgtcga
     2281 tcatgatata agaatcttca tagggtactg ctgcctgttc ttttctttct ttgatttcca
     2341 gcaccacctt atcgggaaga gagcgtctta tcttcacatc atatatatat gaattctttt
     2401 tcatattttc gcgtgtcttc ctggctctta acgcaaatat attctctcct ttttttattc
     2461 ccgacatttt caatatctgc tgttttgtat agttattagt gcctttgact tcgatctttt
     2521 gtatatcaaa atattcggag gacagcaaaa agaaaagtcc cgtgcagagg gctgcaaaca
     2581 aaaggaactt caaaaaataa tgtttctttt ttcttttctt cctcacatgg gtcatatctg
     2641 ctcccgatct gctatataat gacaaatcat ttctgccgca tcaacaggtg cgcatttctc
     2701 agcgcttttt gccatttttg aaagagcctc atcgtctttt atgagttttt ctatttcctg
     2761 ttccagagtc tcttccctca tgtttttttc ttccatcaat atggctgcgc ccttctctga
     2821 aaggaccttt gcattgaatg tctgatggtc accggtaact atgggcgagg gtataagtat
     2881 tgagggtttt ccgcatacag ctatttcaga aacagttatg gctccgcttc tgcttattac
     2941 caaatctgcc gcattcagat atttatgcat atcatttatg taactgataa catttacatt
     3001 ttctttgagt cttatgccct tttcccccag ttcattcatg accggcatat gataatagct
     3061 tccggtggca aaaaatatct gcatgttttc ccggccgttg tatttttcta ttacacttat
     3121 catcgatttg tttattctgc ctgccccctg gctgcctccg aaagccagaa gcacaaaatc
     3181 tttctgcttt atgcccagtt cttttctggc ctcttttttg gcttctttct tatcggcaga
     3241 aaaaaattct tttcttacgg ggttccccgt aacaatatgc ttatcaggat atttgaaacc
     3301 tttgcccgct tcttcgaagc ccaggaatac tttttccaca tgcttttcca tcattctgtt
     3361 tgccaggccg ggcatcgcat tctgctcctg tatatatgtc tttatcccct ggaggtgtgc
     3421 ggcctttaat gccggagcac agacataacc tccggtgcct atcacagcat cggggcaaaa
     3481 ctgctttatg attttgccgg cgtctttcct ggcttgcaga tactccctgg aaacattgat
     3541 attcctgaaa agatttttcc tgtcgaggcc atgcactttt atgaattcta ttttataacc
     3601 ggctgccggt acaagctcat tttccttgcc cctttttact cctataaaca tgatctcgga
     3661 ggaaggagat ttttctttta ttttatcggc aatggctatg gccggatata tatgtcctcc
     3721 cgtaccgccg cctgtcataa taactttcat tttatatcct gcctttctgc attttcgttc
     3781 tttgatatat taagcagtat gcccatagac cccataaata aaagcaacgc attccctcca
     3841 tagcttacga agggcagcgt cactcccgtg ggtggcatca gagaggtcac taccgccaca
     3901 ttcattatta tctgaagccc cagcattatt gttatgcccg aggccaacaa catgccgaaa
     3961 cggtcgggtg cggcaactgc tatccttatc cctctccata tcaaaatcaa ataacatgtc
     4021 atcagaagta ttattcctac gaatcccagt tcctctccta ttattgccag tatgaaatcg
     4081 ttctggggtt ccggcagata tagagttttt tgtacactgt tgccaagacc aaggccgaaa
     4141 aggcctcccg agcccagtgc gagaagggac tgtaccacct gatatcccgt accttgggga
     4201 tcggaaaaag gatccagaaa actcattact ctcgccattc tgtatccggt atcatcgctt
     4261 attataaggg ctgccgccac ggttgcccct gctcctatca ttcctgcaaa atatataagg
     4321 tttatgcctg ccacaaacat catggcgaaa attatgccgc cgattatcag ggcggtggac
     4381 atattgggct gcatcatgac aagccctccg cacaaccccg caagaaatat caagggaagc
     4441 acgcctgtga aaagcgattt gataacattg ctttttcggg aaaggaacca tgccacaaat
     4501 attatgaccg ctatttttgt tatctctccg ggcattaccg ttacgggtcc cacatctatc
     4561 catcttgacg cattgttgat ctctgttccc acgggagtaa gcaccagtgc cagaagtcct
     4621 atgcttaata tgatccccgg cagtgcgatt tttttgtaga acctgtaatc aaccacagta
     4681 agaagcatca taagagcaaa acctgccgtt gcccatataa tgtctcttat aagatagcta
     4741 tagggagttc ctccctcatt tatggaactg taataactgg aactgaagac catgataact
     4801 ccgaaaataa ccagaacaag agtcatgatc gtcaatacga aatcacctga ctgtaattgt
     4861 atcccgtgct ttttcatcag ctttccaatc tctttacgca gtctttgaaa tgctctcccc
     4921 tttgctcgaa attagtatac atgtcccagc ttgcacaggc gggagaaagc aacactgtat
     4981 ctccgggtct tgccagccta aacccttctc ttacgcatgc ttccatatcc ttgactatta
     5041 ttgtgtttgt aaaaccagtt ttttcggcgg catcctttat cttcacagct gtttttccca
     5101 taagaacggc gaacctgact ttgtctccga aggatcttat aagttcctca tatgatgaac
     5161 ccttatcata tccgccggct atcaggatca cattgccctt catagcctct attgccttta
     5221 ttgaggcatc tgtattggtg ccctttgaat cgtttacaaa acggactccg tccacttctc
     5281 ctacgaattc tatcctgtgt tccactccct tgaattcctt cagggtattt gtgatgactt
     5341 ccgaatctat tcccgcaaaa tatgcaactg ccatggcagc aagagcattc tcgacattat
     5401 gggctccggg tacataaagc tcttccgtac cgcaaaactc cgtctttttt ccttcttcat
     5461 ccacaattac catcttcccg tcatcaacaa aagctcccag atcaagcttt tcggtcctgc
     5521 tgaaaggaat gattttcgcc tttgctcccg atgcaagttt catgcattct ttgtcatcat
     5581 gatttatgac cacatatccg tcctcttgct gattctcgaa tatcctggct tttgcagttc
     5641 catatttgtt tatgtctcca tgcctgtcca ggtgatccgg cgtaaggttc agtatagcag
     5701 agatctccgg cttgaaatct ctgatcgttt ccagttggaa actggacact tcagtcacca
     5761 gccatgtatc atcatcagca tttattgcct ttgatattac tgcaacaccg atattcccca
     5821 ctactgatgt ttttcttccg gcatttttga atatttctcc ggtcaaagct gttgtggtcg
     5881 tcttcccgtt cgttccggtg atggccacat atctgccatt gcctgtcctg tatgctatct
     5941 ccagttcccc gattatttca gctccgtttt cctttgcctt ttttatgaat cccatatccg
     6001 gtctcactcc cggactcaat atgagcagat caaaatactc attttcatcc ggagtaacgc
     6061 ccaaataaca ttcaacttct ctgctttcca gaagcccaag cagctgtccg tccagttctt
     6121 cgcgcttttt cacgtcctgt acatatactt tcgcgccaag tttctgcagc gcctgcactg
     6181 cagctattcc tgatttgcca agccctacaa caaggacttt tttgcctttt atttcttcca
     6241 tgtcaaattc tccccactgt ctttacagga tcaaaataca ataaacatta ccaacgcaca
     6301 gcaaatgaat gtaagcagcc aaaaaattat gacgaccttt gtttctttca taccgccc
//
LOCUS       DAONHK010000056         6298 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_9577_length_6298_cov_4.750761, whole genome shotgun sequence.
ACCESSION   DAONHK010000056 DAONHK010000000
VERSION     DAONHK010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6298
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9577_length_6298_cov_4.750761"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1005)
                     /locus_tag="PLM92_03235"
     CDS             complement(<1..1005)
                     /locus_tag="PLM92_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007364489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HPR24187.1"
                     /translation="MQKKGCPYGTHRVISPKGVLPQPADVVDNDMEIYDNEVLIDVQT
                     LNIDSASFTEIERRANGDIEEIKKTMKAIVEKAGKHKNPWTGSGGMLIGTVKETGENY
                     VGDIKPGDKIATLVSLSLTPLVIEEIYDVRPEIDQVDIKGQAILFQSGIYAKLPEDMP
                     EGLALSALDVAGAPAQTRKLVHEGDTVLVIGGGGKSGMLCLYEAKKQAGPKGKVICAA
                     HSQKSLDRAKSLDLADEYFASDATDAVGIYNKIMELTDGKLADVVINVVNIENTEMGS
                     ILACKDDGVVYFFSMATSFTKAALGAEGVGKDVTMIVGNGYCKGHADVTLEDLRESKP
                     L"
     gene            complement(1220..3286)
                     /locus_tag="PLM92_03240"
     CDS             complement(1220..3286)
                     /locus_tag="PLM92_03240"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPR24188.1"
                     /translation="MENQYGMARVKEPRQVLPTSAWRLDNKREIYPNEIRIALEKIHI
                     EGTSFRQICMESGDNDELIRSKIIDIVIRRGKLHNPVTDTGGLLYGTVEEIGTGYNNE
                     KNLKPGDKVLYNASLASIPIYIGRVKEIDRAYNQIEAEGYAIVHDKVPVVRKVPGVPV
                     DLLLYTLNESGTLYRISSNAVGKKKILVAGNNLMLNLLFGHTIRKAAGPEASIISVLD
                     KSINTVVRGEKIDWLVKRTFDEIHYLNILRPMECMETLKADGLFDMSVNCADIPGAET
                     LNVLATRKGGTVFFANLINNYNIALYITESVARHMNIKCAEGYLEEYDEFDMELVKEL
                     APYFQGATVEEHREKKHIGDVIDDTEQKRALAEDFISESRSMGLVLQEIMSVAKYDCN
                     VLISGDTGVGKEKVANIIQKNSRRKMHPFVKVNCASISPNLMESEFFGYEPGSFTGAS
                     TGGKKGYFEVADNGMVFLDEIGELPPDIQAKLLRVIQDGEFLRVGGTKPIKTNVRIIS
                     ATNRDLEELIEKNLFRRDLYYRLNVFPIKVPSLSERKEDIQPLINHFIKQYGEKFGIS
                     CGIDEDAAEYLEKCQWPGNIRELENVIQRLLINARGSNIKLMDVMKEVHGDLFESSKI
                     EFPSDIGGEIDDEGAKLKLDMLVGDFEKQIIRYACEKYGSTRKAARAIGISQTQLVRK
                     KNKYGI"
     gene            3429..3818
                     /locus_tag="PLM92_03245"
     CDS             3429..3818
                     /locus_tag="PLM92_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009259558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold domain-containing protein"
                     /protein_id="HPR24189.1"
                     /translation="MEKITSTIRLRMSHKDAHYGGSLVDGAHMVHLFGDVATELLIKA
                     DGDEGLFLRYDDVEFLAPTYAGDYIEAYGEITEFGNTSRKMSFEARKVVAARPDISPS
                     AADSLEEPIIVCRAKGICVTPKESKRK"
     gene            complement(3831..4652)
                     /locus_tag="PLM92_03250"
     CDS             complement(3831..4652)
                     /locus_tag="PLM92_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008821427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HPR24190.1"
                     /translation="MEKLIIAAAICGAEVTKEHNPAVPYTVSEIVREAKSAYDAGAAV
                     IHLHVREDDGTPTQSKERFQECVEAILKECPDAIIQPSTGGAVGMTDMERLQSTEISP
                     TPEFATLDCGTCNFGGDEIFVNTDNTIFNFAKTMKERGIKPELECFDKGMVDIAMKAH
                     KKGLLEDPMHFDFVLGVQMTATVRDLVFCVGSIPPGSTWTGTGIGRYEFEMATASIIM
                     GGHVRVGFEDNLFIEKGVLARSNGQLVEKVVKIAKLLGREIASPDEARKILGIKK"
     gene            complement(4664..5701)
                     /locus_tag="PLM92_03255"
     CDS             complement(4664..5701)
                     /locus_tag="PLM92_03255"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HPR24191.1"
                     /translation="MTYRIEKKVIDKFLENQFYYRDFCGMAAGIGSSAKGLEYKKALG
                     ISSVKTGSPLTEGSVFHMASVAKLFTATGIMLLQEKGMLDIDEKLISYMPWFKMADKR
                     YEKITIRQMLTHTSAMPDVSDYHWEQALTEEKALKDYALSPEVINAHLLRDPQEGKFA
                     YSNMAYDLLGLLIKEVSGESYENFIANNIFGPVGMKESTLLTMERKDRDKMADPHDKN
                     ADKKFEVLPHYPYNRSHSPSSTLTSNMDDMAKWASAWLSGRFPKVETKEEMWKEYALV
                     RNNGEKMGLGWFSRSQSGRTLYGHEGMDDGFRASFWLCPEEDLFIMIFANMSKGPVKK
                     VNKELFELLIS"
     gene            complement(5757..>6298)
                     /locus_tag="PLM92_03260"
     CDS             complement(5757..>6298)
                     /locus_tag="PLM92_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPR24192.1"
                     /translation="MKLEWKEIKKSKKALEEGLAKTDKKISDRKKKIGDASDKQDDKG
                     EKSFAKAGPKDKAMDEEAETGRPASDEKEGIPKEEERDWSEEIEKCEEGSVYYHWAYG
                     KMEVLAKEGDYIYMKLMDKKGCRHEWLTKNNAAVEIDEKKEEAKEFSLFSIGRWLFPD
                     IADVEVIDPEKAHRSFIKN"
BASE COUNT         1371 a         1603 c         1260 g         2064 t
ORIGIN      
        1 caaaggtttg ctttctctca ggtcttccag ggtcacgtcc gcatgtccct tgcagtagcc
       61 gttgcctact atcatggtaa catcttttcc tactccctct gcccccagag cagcctttgt
      121 gaaactggtg gccatggaga agaagtaaac tactccgtca tccttacagg caagtatgga
      181 tcccatttct gtattttcta tgtttacaac gtttattacc acatccgcca gtttgccgtc
      241 tgtaagctcc atgatcttgt tgtatattcc aacggcatcc gttgcatctg atgcgaaata
      301 ctcgtctgca agatccaggc tctttgccct gtcaagggat ttctgtgaat gagctgcaca
      361 aatgactttg ccctttggtc ctgcctgttt ctttgcttca taaagacaaa gcatccctga
      421 cttgccgccg ccgccgataa caagtacggt gtcgccttca tgcaccagtt ttctggtctg
      481 agccggggct cccgccacgt ccagcgctga aagtgccagt ccttcgggca tgtcttctgg
      541 aagctttgca tatatgccgc tctggaaaag tattgcctgt ccctttatat caacctgatc
      601 tatctcgggt cttacatcgt atatctcctc tatcacgagg ggtgtcaggg aaagagatac
      661 cagcgttgct atcttgtcgc cgggttttat gtctcccaca taattttcgc ctgtctcttt
      721 gactgttcct atcagcattc cgccggaacc tgtccatggg ttcttgtgct tgccggcttt
      781 ttctacaatg gctttcattg tttttttgat ctcttcgata tcgccgttgg ctcttctttc
      841 tatttcggtg aatgaggcgg aatcgatatt aagtgtctgt acatcgatca gaacttcatt
      901 gtcatagatc tccatatcat tgtctacgac atctgccggc tgcggcagta ctccctttgg
      961 tgaaatgact ctgtgtgttc cgtatgggca tccttttttc tgcatttttt tatcctcctt
     1021 ctgaccggtc tgatgccggt tttttaacag ttttgtttac cttataacac agcaaagaac
     1081 gtgccatata agacacgttc aaaaaaagat attttttcgt tggatttcaa gggttctgtg
     1141 gtttttcgaa aatttttgtc catggttttt gagccattgg gtgttcacgt gctttgtgat
     1201 gtgaacatat attggttcat catatgccgt atttgttttt tttgcgcacc agctgggtct
     1261 ggctgatgcc gatggccctt gctgctttcc ttgttgagcc gtatttttca caggcatatc
     1321 tgataatctg cttttcgaag tcccccacca gcatatccag cttcagtttt gctccttcat
     1381 catcaatttc accgccaata tcgcttggga actcgatttt gctgctttcg aaaagatctc
     1441 catgcacttc tttcatgaca tccatgagct ttatgttgct gcccctggca tttataagaa
     1501 gcctctgtat gacattttcc agttctctta tattgccggg ccactggcat ttttcaagat
     1561 attccgcagc atcttcatct atgccgcagg atatgccgaa tttctctccg tactgtttta
     1621 tgaaatggtt tataaggggc tggatgtctt ccttcctttc cgaaagggac ggcaccttta
     1681 tggggaaaac attgagcctg taataaaggt ctctcctgaa aagattcttt tcgataagtt
     1741 cttcaagatc tctgttggtg gccgaaatga tacgcacatt ggtttttatg ggttttgtgc
     1801 ctcccaccct cagaaactcc ccgtcctgta tgactctcag aagctttgcc tgtatatccg
     1861 ggggcagttc accgatctca tcaagaaaaa ccataccgtt gtcggccact tcgaaatatc
     1921 ctttctttcc tccggtgctg gcaccggtaa acgatcccgg ctcataaccg aaaaactcgg
     1981 attccataag attgggcgaa atggatgcac agttgacttt gacaaaagga tgcatcttcc
     2041 ttctgctgtt tttctgtatt atattggcta ctttttcttt tcctactccc gtatcaccgc
     2101 ttataaggac attgcagtca tatttggcga cactcattat ctcctgcaac accagaccca
     2161 tggatctgct ctcgctgata aagtcttccg ccagggctct tttctgctcc gtatcatcaa
     2221 ttacgtcgcc tatgtgcttt ttctcccggt gctcttccac ggtggctcct tgaaaatatg
     2281 gggcaagctc ctttaccagc tccatatcaa actcatcata ctcttcaaga tatccttccg
     2341 cgcattttat gttcatgtgc cttgccacgg actcggttat ataaagggct atattatagt
     2401 tgtttatcag atttgcgaaa aaaactgtcc cgcccttcct ggtggccagc acattcaggg
     2461 tctctgctcc gggaatatcg gcacagttca cggacatatc gaaaaggccg tcggctttta
     2521 aggtctccat gcattccatg ggtcttaaga tgttcagata gtgtatctcg tcaaaggtgc
     2581 gtttcaccag ccagtctatc ttttcccccc ttacaactgt atttatgctc ttgtcaagaa
     2641 cgctgattat ggaagcttcc gggcccgccg cttttctgat ggtgtgtccg aaaagaagat
     2701 tcagcatcag gttattgcct gccacaagta tctttttctt tcccaccgca ttgctgctta
     2761 tcctgtaaag ggtaccggac tcattaagag tataaagaag aaggtcaaca ggcactcccg
     2821 gcactttccg aaccacgggc accttgtcat gaacgatggc atagccttcg gcttctatct
     2881 gattgtaagc tctgtctatc tctttcaccc tgcctatata aattggtatt gaggcaaggg
     2941 aagcgttata aaggaccttg tctcccggtt tcaggttttt ttcgttattg tatcccgtac
     3001 ctatctcctc cacggtaccg taaagaagcc cgccggtatc cgtaacggga ttgtgcagtt
     3061 tgccccttct tatgacgata tctattattt tgctgcggat aagctcatcg ttatctcccg
     3121 attccatgca gatctgcctg aaactggtac cttcaatgtg tattttttca agagctatcc
     3181 ttatctcgtt ggggtatatt tcccttttgt tatcaaggcg ccaggcggat gtgggcagta
     3241 cctgcctggg ttcttttact ctggccattc cgtattggtt ttccattata tatctactcc
     3301 ttatcaggtg aaacaaaatc ggttcacctt tgctttatca taccctttta caataagatt
     3361 tgcaattgca aatcaaaaat tatattatga aacctgaaag ctcacaatct taacaaaagg
     3421 agatcgatat ggaaaagata acttcaacta taagactgag gatgtcccat aaggatgccc
     3481 attacggcgg cagtctggtg gacggggccc acatggtcca tctgttcgga gatgtggcaa
     3541 cggaacttct tataaaggcc gacggtgatg aaggtctgtt tcttagatat gacgatgtag
     3601 agtttctggc acccacatat gccggtgact atatcgaagc atacggcgaa ataaccgaat
     3661 ttggcaacac atcaagaaaa atgtctttcg aagccaggaa agtggtggca gcaagacctg
     3721 atatcagccc ttccgccgca gattccctgg aagaacccat aatagtatgc agggcaaagg
     3781 gcatatgtgt gacccccaaa gagtcaaaaa gaaaataact gccgaggcag ttattttttt
     3841 attcccagta tttttctggc ttcatcgggg cttgctattt ctctcccgag aagttttgct
     3901 atttttacga ctttctccac cagctgaccg ttgctcctgg caagaactcc tttttcaata
     3961 aacagattgt cttcgaatcc gactcttaca tgtcctccca ttattatgga agccgtggcc
     4021 atctcaaatt catatctgcc tatccctgtg cctgtccagg tgctgcccgg aggtatgctt
     4081 cccacacaga atacaagatc tcttaccgtg gcagtcatct gcactcccag aacgaagtca
     4141 aaatgcatgg ggtcttccag aaggcctttt ttgtgtgctt tcattgcgat atccaccatg
     4201 cccttgtcaa agcattccag ttccggcttt attcctcttt ctttcatggt tttagcgaaa
     4261 ttgaaaatcg tattatccgt attaacgaat atctcatctc cgccgaagtt gcatgtgccg
     4321 cagtccagtg tggcaaactc cggagtcggg cttatctctg tggactggag cctctccata
     4381 tcagtcattc ctaccgctcc gcccgtggaa ggctgtatta tggcatcggg gcattcctta
     4441 aggatcgcct ccacacactc ctgaaaacgt tccttgctct gtgtgggtgt gccgtcatct
     4501 tctcttacat gaagatgtat caccgccgct cccgcatcat atgcagactt ggcttccctt
     4561 actatctccg aaacagtgta gggcactgca ggattatgct cctttgtgac ctctgctccg
     4621 catattgccg ctgctattat cagtttttcc attttttatc ctcctatgat ataagcaatt
     4681 caaataattc cttatttact tttttgaccg ggccctttga catgtttgca aaaatcatta
     4741 taaacaaatc ttcttcggga cacaaccaga aactggctct gaagccgtca tccattccct
     4801 catgtccgta aagggtcctt cccgactgac tacgtgaaaa ccacccaagt cccatttttt
     4861 ccccgttgtt tcttactagg gcatattcct tccacatttc ttcttttgtc tccaccttgg
     4921 ggaatctgcc cgaaagccat gcggatgccc atttggccat atcatccata ttggatgtca
     4981 atgtggaact gggggaatga ctgcgattgt aaggatagtg gggcagtact tcaaattttt
     5041 tgtctgcatt tttatcatga gggtcagcca ttttatccct gtcttttctt tccatggtca
     5101 gcagtgtgga ctccttcatg ccgacaggtc cgaatatgtt atttgcgata aaattctcat
     5161 agctttctcc cgacacctct tttataagaa gtcccagaag atcataggcc atattgctgt
     5221 atgcgaactt gccttcttgc ggatcccgaa gcaggtgggc atttataact tccggagaaa
     5281 gagcataatc cttcagggcc ttttcctctg tgagcgcctg ctcccagtga taatccgata
     5341 catcgggcat tgccgatgta tgggtgagca tttgccttat ggttattttt tcgtatctct
     5401 tatcggccat tttgaaccag ggcatgtagc tgatcagttt ttcatctata tccagcatgc
     5461 ccttttcctg aagcaacatt atccccgtgg cagtaaaaag ctttgccacc gaggccatat
     5521 gaaagacaga gccctctgta agggggctgc cggtctttac actgcttatc cccagggctt
     5581 ttttgtattc caggcccttt gcggaacttc ctatgcctgc tgccattccg cagaaatctc
     5641 tgtaataaaa ctggttttca agaaatttgt caataacttt tttctctatc ctgtatgtca
     5701 tatttgcctc tcctataaga atggctgcgc ttttgcggca cagcctttct ttgttatcaa
     5761 ttctttatga aacttctatg tgctttctcc ggatcgatca cctcaacatc cgctatatcc
     5821 gggaagagcc atctgcctat ggaaaacagg gaaaattcct tggcttcttc ttttttttcg
     5881 tcaatttcca ccgcagcatt gttcttggtc agccattcat gccggcatcc ctttttgtcc
     5941 ataagcttca tgtagatata atctccttct tttgccagga cttccatctt tccgtatgcc
     6001 cagtgataat agactgaacc ctcttcgcat ttttcaattt cttcgctcca gtctctttct
     6061 tcttcttttg gaattccttc tttttcgtcg cttgcaggtc tgcctgtttc tgcttcttca
     6121 tccatggcct tgtccttcgg accggccttt gcaaaggact tttccccctt atcatcctgt
     6181 ttgtcggaag catcgcctat cttctttttt ctgtcgctta ttttcttgtc tgtctttgca
     6241 agaccttcct caagggcttt tttgcttttt ttgatttctt tccattccag cttcatcc
//
LOCUS       DAONHK010000057         6208 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_9738_length_6208_cov_4.469527, whole genome shotgun sequence.
ACCESSION   DAONHK010000057 DAONHK010000000
VERSION     DAONHK010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6208
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9738_length_6208_cov_4.469527"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..791
                     /gene="metA"
                     /locus_tag="PLM92_03265"
     CDS             <1..791
                     /gene="metA"
                     /locus_tag="PLM92_03265"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005920290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HPR24193.1"
                     /translation="TKIATETQLARVLRNSPLQVELTLLAMDSHESKNVSQSHMRDFY
                     KTIDEVESERFDGMILTGAPVEQIAFEEVDYWKELTGILEYAKENVYSSMFLCWGAQA
                     ALYHHYGINKYQMDKKMFGIFETKVVRPLNPLMRGFDEIFYSPHSRHTEVIKSDILKH
                     PELRILAESQEAGPHILSTENGRQIFVMGHQEYDKETLASEYFRDRSKGLEISVPKNY
                     FKENDPEKEPMFRWRSHANLLFSNWLNYYVYQETPYNIDEIKAD"
     gene            860..2368
                     /locus_tag="PLM92_03270"
     CDS             860..2368
                     /locus_tag="PLM92_03270"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009063861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HPR24194.1"
                     /translation="MSNCFREPSRTFNEYLLVPGYSGKECNPENVELDAAVTRYKKGE
                     KPKIKMKIPLASAIMQSVSDDNMAIALAKEGGISFIYASQPIEEQAVMIKRVKSHKAG
                     FVKSNTNIRPDQTMKDVIELKDRTGHSTTAVTEDGTSEGRLVGIVTSRDYRVSRMDPE
                     TKVSEFMTPFDKLVYAKEGVSLSAANDILWDHKLNALPIIDENRRLTHFVFRKDYDSH
                     KAHPNEILDDDKRYVVGAGINTRDHDERIPALIDAGADVLCIDSSEGHSQWQEETIGF
                     VRKKYGEDVKIGAGNVVDSEGFRFLAEAGADFVKVGIGGGSICITREQKGIGRGQASA
                     VMDVAEARNKYYEETGIYVPICSDGGIVYDYHMTLALAMGADFLMLGRYFARFDESPG
                     AKLNVNGNYVKEYWGEGSARARNWQRYDMGGESKLSFEEGVDSYVPYAGGLSDNVRQS
                     LQKVKSTMCNCGVINIPDLQKNAKLTLVSATSIVEGGAHDVIPKETANTSNR"
     gene            2417..3967
                     /gene="guaA"
                     /locus_tag="PLM92_03275"
     CDS             2417..3967
                     /gene="guaA"
                     /locus_tag="PLM92_03275"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003410698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HPR24195.1"
                     /translation="MQHETILVLDFGGQYNQLIARRVRDLNVYAEVLPYTLSAEEIQK
                     KAPKGMILTGGPNSAYGPGALEYDKGIFELGIPILGICYGAQVLAHGLGGEVAKAPAS
                     EYGRTEVDIDKNGRLFAQVPEKTIAWMSHTDYIKQAPKGFKVTAETPICPVAAMEDVS
                     RGFYATQFHPEVMHTKEGIKILRSFVKDVCGCSGDWHMGSFVENAIETVREKVGSGKV
                     LCALSGGVDSSVSAVILSKAVGKQLTCVFVDHGLLRKNEADEVEAVFGKGGHYDLDFI
                     RVNARQRFYDKLAGVTDPEKKRKIIGEEFIRVFENEAKKIGKVDFLVQGTIYPDIIES
                     GPGDSATIKSHHNVGGLPEHVDFKDIIEPLKMLFKDEVRKAGMELGIPKHLVMRQPFP
                     GPGLGIRIIGDITEEKVKIVQEADAIYREEVAAYDGGKLAPTLGQYFAALTNVRSVGV
                     MGDFRTYDHAIALRAVETSDFMTAEAAKLPWDLIEKVTTRIVNEVPHVNRVFYDCTNK
                     PPGTIEFE"
     gene            4207..5391
                     /locus_tag="PLM92_03280"
     CDS             4207..5391
                     /locus_tag="PLM92_03280"
                     /inference="COORDINATES: protein motif:HMM:NF033550.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HPR24196.1"
                     /translation="MLYSDFIEELTGLKDLIVKKVKNVYGQQHIYAVMHKRIHACPAC
                     GTNTSKVHDYRTQTIKDISSYGQTTFIHLRKRRHVCPVCGKRFYEEVEFLPRYHRMTN
                     RLYAYILEQFHDVLSMKYIAGRCNVSITTIMRIFNMVQYPRPKLPEVLSIDEFRGNAD
                     GEKFQCILTDPRNRKVVDVLGKRRAEDLYAYFMYFDKSERNQVKYVSMDMSTLFRSVV
                     KTCFPEAKIVADKYHVVRQVTFSLDHVRKKIQKEFSKDRRLHFKHSRHILLKPSNSLT
                     EDERDRLSVMLQASEYLREAYLRKMEFEKVTKCKTKGSAKKELAKWIVLTQNSGIEEY
                     KTSCMTFIKWADEIAEAFGSNITNGYTEGVNNKIKVLKRVSFGVRNFSRFRNRILHIM
                     TV"
     gene            5680..5826
                     /locus_tag="PLM92_03285"
     CDS             5680..5826
                     /locus_tag="PLM92_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008789314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPR24197.1"
                     /translation="MFDKKIKMNVTDDESQLILFSLVELRNNLIKEGRYTDAVDDLIL
                     KLFG"
     gene            5948..>6208
                     /locus_tag="PLM92_03290"
     CDS             5948..>6208
                     /locus_tag="PLM92_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPR24198.1"
                     /translation="MKTTEETIKVIMTGLCSREDDESEYCPLIEHLAAEAVEDFEFCV
                     QEEMIDPEAAVHFMCLVDSMYRREAYFEGTKALGEIFRYAGRE"
BASE COUNT         2049 a         1246 c         1532 g         1381 t
ORIGIN      
        1 ccaccaaaat agccacagag acccagctgg ccagagtgct gcgcaattct cccctgcagg
       61 tggagcttac ccttctggcc atggacagcc atgaatcaaa aaacgtttca caaagccata
      121 tgagggattt ttataagacc atcgacgagg tggaaagcga gcgtttcgac gggatgatac
      181 ttacgggagc cccggtggag cagatagcct ttgaagaggt ggattactgg aaggagctca
      241 cggggatact ggaatatgca aaagaaaacg tttacagcag catgtttctt tgctggggag
      301 cccaggcagc cctttatcat cattacggca taaacaagta tcagatggac aaaaaaatgt
      361 tcggtatttt tgagactaaa gttgtaagac cgttaaaccc ccttatgagg ggatttgatg
      421 aaatttttta ttcgccccat tcccgccata cagaagttat caaaagcgat atcttaaaac
      481 accccgaact tcgaatattg gcagaatccc aagaagcggg gccccatata ctttccacag
      541 aaaacggaag gcagatattt gtgatgggac atcaggaata tgacaaggag accctggcat
      601 cggaatattt cagagatcgc agcaagggac ttgaaataag cgttcccaaa aactacttca
      661 aagaaaacga ccccgaaaaa gagcccatgt tcaggtggag gagccatgcg aaccttcttt
      721 tcagcaactg gctcaactat tatgtatatc aggaaacgcc gtacaacatc gacgagataa
      781 aggccgactg aaattgaaaa aacaacattt ttgttataga ataaatcgaa agatccaaag
      841 aacaaaacag gaggaagata tgtcaaactg tttcagagaa ccatcaagga cattcaacga
      901 atatctgctg gtgcccggat actccggaaa ggaatgcaac cccgaaaacg tggagcttga
      961 tgcagcagtt accaggtata aaaaaggcga aaaaccaaaa attaaaatga agatcccgct
     1021 ggcgtcggca ataatgcagt ctgtgtctga tgacaacatg gccatagctc ttgccaagga
     1081 aggggggatc tcttttatat acgcttccca gcccatagaa gagcaggccg tcatgataaa
     1141 aagagtcaaa agtcataaag cgggctttgt aaagagcaac actaatataa gacctgatca
     1201 gaccatgaaa gatgttattg aactgaagga ccgcacggga cattcaacta cggcagtcac
     1261 agaagacggc acctccgaag gccggctggt gggcatagtc acaagccgtg attaccgggt
     1321 aagcaggatg gatcccgaaa caaaggtgtc ggaattcatg actccctttg acaaactggt
     1381 atatgcaaaa gaaggagtca gtctttctgc ggccaatgat atactctggg atcataagct
     1441 caatgctctg cccataatag acgaaaaccg caggctcacc cattttgttt tccgcaagga
     1501 ttacgattct cacaaggctc acccaaatga gatactggat gacgacaaac gatatgtggt
     1561 gggagcagga ataaatacaa gggatcatga tgaaaggatc cccgcactta tagatgccgg
     1621 ggcggatgtg ctctgcatcg attcttcgga ggggcacagc cagtggcagg aagaaaccat
     1681 cggtttcgtg agaaagaagt acggagaaga tgtgaaaata ggagccggta atgtggtgga
     1741 cagcgaagga ttccgtttcc tggcagaggc gggagccgac tttgtgaagg tgggtatcgg
     1801 aggcggttcc atctgtatca ccagagaaca aaaaggcata ggaaggggac aggccagtgc
     1861 tgttatggat gtggcagaag caagaaacaa atattacgaa gaaacaggga tatatgtgcc
     1921 catatgctcc gacggaggga tagtatatga ctatcacatg accctggccc tggccatggg
     1981 agcggatttt ctcatgctgg gcagatactt cgccagattt gacgaatctc ccggagccaa
     2041 gcttaatgtg aacggcaatt atgtgaagga atactgggga gaggggtctg caagggccag
     2101 gaactggcag cgctacgata tgggaggaga aagcaagctt tcctttgaag agggagtgga
     2161 ttcctatgtg ccctatgcgg gaggtctttc tgacaacgta agacagtctc tgcaaaaggt
     2221 gaaatcaacc atgtgcaact gcggcgtgat caacataccc gatctgcaga aaaatgccaa
     2281 actgactttg gtttccgcca ccagcatagt tgaaggcgga gcccatgatg tcataccaaa
     2341 ggaaacagcc aatacatcaa acagataaaa cagaagcaga aaaaaagaaa cagatagaag
     2401 agaacaggag aaaaagatgc agcacgagac catactggta ttggatttcg gcggacagta
     2461 caaccagctg atagcacgcc gtgtcagaga ccttaatgta tatgcggaag tccttccata
     2521 tacattatca gcagaggaaa tacaaaaaaa agctcccaag gggatgatcc ttacgggagg
     2581 gcccaacagt gcatacggcc ccggtgccct tgaatatgac aaaggcatat tcgagctggg
     2641 gatacccata ctgggcatat gctacggagc ccaggtgctg gcccacgggt tgggaggaga
     2701 agtggcaaaa gctcccgcca gcgagtacgg ccgcacagag gtggatatag acaaaaacgg
     2761 cagacttttt gcccaagtac cggaaaaaac catagcatgg atgagccata cggactatat
     2821 aaaacaagcg cctaagggtt ttaaagtgac cgcagaaaca cccatatgtc ccgtggctgc
     2881 aatggaagat gtttcaaggg gattctatgc gactcagttc catccggagg tgatgcacac
     2941 taaggaaggc atcaagatcc tccgaagttt tgtaaaagat gtatgcggat gcagcggcga
     3001 ctggcatatg ggatccttcg tggaaaatgc catagagacc gtcagggaaa aggtgggcag
     3061 cggcaaagtt ctctgcgccc tttccggcgg agtggattct tctgtttccg ccgttattct
     3121 gtcaaaggca gtgggcaaac agctcacctg cgtatttgtg gatcacggac tcctgagaaa
     3181 aaatgaagct gacgaagtgg aagcagtttt tggtaaaggc ggccattacg accttgactt
     3241 cataagagta aatgcccggc agagatttta tgataaactg gccggagtca cggatccgga
     3301 gaaaaaaaga aaaataatag gagaagagtt cataagagtt ttcgaaaatg aagcaaaaaa
     3361 aataggtaaa gtggactttc tggtacaggg gaccatatac ccggatataa tagaaagcgg
     3421 cccgggagac tccgccacca tcaaatccca tcacaacgtg ggcggactgc ctgaacatgt
     3481 ggatttcaag gacataatag agcccctgaa aatgcttttt aaagatgaag ttcgaaaagc
     3541 aggcatggaa ctgggcatcc cgaagcatct ggtaatgcgt cagcccttcc ccggccccgg
     3601 cctgggtata aggataatag gagacatcac cgaagaaaaa gtcaagatag tccaggaggc
     3661 ggacgccata tacagagaag aagtggcagc ctacgacgga ggcaaacttg ctcctactct
     3721 cgggcagtat tttgccgccc tgaccaatgt gcgttccgtg ggagtgatgg gggattttcg
     3781 cacatacgat catgccatag ccctgagggc agtggagaca tcggacttca tgacggcgga
     3841 agctgccaag cttccctggg atctcatcga aaaagtcacc accaggatcg tcaacgaagt
     3901 gccccatgtg aacagagtct tttacgactg cacgaataaa ccgccgggaa cgatagaatt
     3961 tgaatagggc cgagaggctt tcacagttga tagaaccgtt ggaatcactg agattaagcg
     4021 attctacaaa atgccgtggt tctactatgg ttctacaatt ttatagaatt tattggctct
     4081 ataataaatg ttggggtgga gagagaagat ctctgctcca acatttttga tttgtgaaaa
     4141 aaaagagcat atcagcttta aatcctttag aataaagtta caacccaaaa tcaaaaggag
     4201 gctaatatgc tctatagtga ttttatcgaa gaattgaccg gattaaaaga cttgattgta
     4261 aaaaaagtaa aaaatgttta tggacaacaa cacatttatg ctgtaatgca taaaagaatt
     4321 catgcctgtc ctgcttgcgg aaccaataca tcaaaagtcc acgactacag gacccaaacg
     4381 atcaaagaca tttcttcata cggacaaaca actttcatac atctaaggaa aagaagacat
     4441 gtatgccctg tttgcgggaa gaggttttat gaagaagttg aattcttacc caggtatcat
     4501 agaatgacaa acagacttta tgcttacatc ctggaacagt tccatgatgt attatcgatg
     4561 aaatatattg ccgggcgatg caatgtttcc atcacaacga taatgcggat attcaacatg
     4621 gtacagtacc caagacccaa gctcccggaa gtgctttcca ttgacgaatt cagaggcaat
     4681 gcagatgggg aaaaattcca atgcatattg accgatccaa gaaacagaaa agtggtggat
     4741 gtacttggga aaagacgggc tgaagacttg tatgcatact ttatgtactt tgacaaaagc
     4801 gaaaggaatc aagtcaaata tgtaagcatg gatatgagta ctctattcag aagtgttgta
     4861 aagacctgtt tcccggaggc aaagatagtt gcagacaaat atcatgtggt aagacaggtg
     4921 accttttcac tggatcatgt aagaaagaag atacaaaagg aattttccaa ggataggcgg
     4981 cttcacttca aacacagcag acacatactt ttaaagcctt caaacagttt aacagaagat
     5041 gaaagagaca ggttgtcagt aatgctgcag gcatcggaat atctcagaga agcctatctt
     5101 agaaagatgg aatttgaaaa ggtgaccaaa tgcaaaacaa agggatctgc caagaaagag
     5161 ctggcaaaat ggatagttct cacgcaaaat tccggcatcg aagaatacaa aaccagctgc
     5221 atgacattca taaaatgggc agatgagatc gcagaagctt ttggaagcaa tatcaccaat
     5281 ggctatacag aaggggtcaa caacaagatc aaagtcctga aaagagtctc ttttggtgtg
     5341 aggaatttct ccagattcag aaacaggatc ctccatatta tgactgttta acatataaaa
     5401 aagtaagggc ataaagccct tactgaaaaa tcatatatgt gattttattc cgggatatta
     5461 agctccaccc caacatttga catagagcca atttattaag cagcgtatcg ttggtatttc
     5521 aatgaatacg ctgtttttta atgcctcatt gccattgtga tgccaaatgt actcaaagtc
     5581 aaagatggca taggaaatcg tataattgca acttgatatt tctgttagta aaatctagta
     5641 aaagatactc cacatagaac aaagcaggag gtggatatta tgtttgataa gaagataaag
     5701 atgaacgtaa cagacgatga gagccagctc atactcttca gcctggtaga actcagaaac
     5761 aacctcataa aagaaggcag atatactgat gccgtggatg atctcatcct gaagctgttt
     5821 ggatagagaa acttagatcc tttcctgaat aatgatgcct aaggcatatt acattgctct
     5881 gctgaaacat gcagagcttt agtaattttt ggaccggcat caccggggaa aggaagaggt
     5941 aaggataatg aaaactacag aagaaacaat aaaagtaata atgacggggc tttgcagcag
     6001 agaggatgac gaaagcgaat actgtccgct gatcgaacac cttgcagcag aggcagtgga
     6061 agattttgag ttttgtgtac aggaagaaat gatagatcct gaagcagcag tccattttat
     6121 gtgtcttgta gatagcatgt acagaagaga agcgtatttt gaagggacaa aagcgcttgg
     6181 agagatattt cgctatgcag gtagagaa
//
LOCUS       DAONHK010000058         6194 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_9767_length_6194_cov_4.164196, whole genome shotgun sequence.
ACCESSION   DAONHK010000058 DAONHK010000000
VERSION     DAONHK010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6194
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9767_length_6194_cov_4.164196"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1021
                     /locus_tag="PLM92_03295"
     CDS             <1..1021
                     /locus_tag="PLM92_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012064254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HPR24199.1"
                     /translation="PGKEAMEKAGIPIIKLEAKEGLALINGTQIMTAIGVNVLWDAMN
                     LLKVSDIAAAMTGEALNAVKKAYDHKVHDLRGHKGQKDSAENLLKLLDGSDNAHELNK
                     NKVQDPYSLRCVPQVHGASRDAVQYVYEAVSREINAVTDNPLIFPEEDEVISGGNFHG
                     QPMALAFDFLKNAIAELADISERRSERLINPQLSEGLEPFLTKHGGVCSGFMIAQYAA
                     ASMVSENKIYAHPASVDSIPSSGNQEDHVSMGTTAARTAAMILDNSQKVLGIELFAAS
                     QGIWLRGEKGLSKATKAAYDHIRKTVEPVDQDVIMHYEIKKFDEMIKTNEIVEEVEKI
                     TGPLN"
     gene            1036..3060
                     /locus_tag="PLM92_03300"
     CDS             1036..3060
                     /locus_tag="PLM92_03300"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HPR24200.1"
                     /translation="MLTNQKIAEAMTIKLEDRLPEYPKFVEGKRRAPDRGFRLTKEQT
                     KIALKNALRYVPEEFHEVLATEFMEELKTYGRVYAYRYRPEGRIYGKPIEEYKGNCLE
                     GKAFQVMIDNNLDFEVALYPYELVTYGETGQPCQNWLQYRLIKKYLEELTRDQTLVIE
                     SGHPLGLFPSKPEAPRVILTNSMMIGIFDNLDDWEIAEEMGVANYGQMTAGGWMYIGP
                     QGIVHGTFNTILNAGRKYLGVPEQGDLTGHTFVSSGLGGMSGAQPKAATIAGAVGIFA
                     EVDRSRIETRHEQGWVDVILSDLDQVFALAKESVQKKESISIAYWGNIVDLLEAAIAK
                     DFKIDLLSDQTSCHNAYNGGYCPVGLTFQKRTEMLHRDRKMFCEMVDASLRRHFEAIK
                     ALTKNGTYFFDYGNSFMKAMYDSGIKEISKNGHDEKDGFIWPSYVEDIMGPLLFDYGY
                     GPFRWVCLSGKPEDLDATDKAAMDCIDPKRRFQDRDNWVWIRDAKKNKMVVGTQARIL
                     YQDAEGRMNIALKFNEMIKEGKIGPVMIGRDHHDVSGTDSPFRETANIKDGSNVMADM
                     AVQCFAGNAARGMSLCALHNGGGVGIGKAINGGFGMLLDGSKRVDEILRSAMMWDVMG
                     GVARRNWARNENALEASATYNEKQKGKAHITLPYIPDENLIEETVGKFVK"
     gene            3082..4323
                     /gene="hutI"
                     /locus_tag="PLM92_03305"
     CDS             3082..4323
                     /gene="hutI"
                     /locus_tag="PLM92_03305"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014117113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HPR24201.1"
                     /translation="MSTTLIKNIGLLQTPIGSHPHRGEKQGENLKIKNAAVFARDGII
                     EEITENGRLPGAFEKEDADEIIDAEGSLVTPGLVEGHTHLVFGGYRQHEIPLKLKGAG
                     YLDILKAGGGILDTVRKTREAGFDELYEKSRGFLDEMMEMGVTTCEAKSGYGLDLETE
                     VKMLEVIKKLNREHRMDLAATFMGAHAVPPEYKGKPDQYIEMICEKILPVVKEKNLAE
                     FVDVFCEEGVFDEEQSRRYLSKAKELGFSLKIHADEIEEMGGSKLAGEIGAVSAEHLI
                     AIGPGGLSAMAKAGVTAMCLPSTSFYLGKDFAPVRKMIEMGIPVATASDFNPGSCPSL
                     NLQFVMNLGYLKYKMMPEEILTAVTINPACAIGKGDLCGTLEKGKKADMVIWNAKDME
                     MLCYRFGSNLAMATIKEGVLI"
     gene            4323..4949
                     /locus_tag="PLM92_03310"
     CDS             4323..4949
                     /locus_tag="PLM92_03310"
                     /inference="COORDINATES: protein motif:HMM:NF016825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HPR24202.1"
                     /translation="MDLADLKVKEYLDVLQSDAPAPGGGSVSALAGAQGIALMMMVAD
                     LTCGREKYSAFEDVCKKVKTEAVGIFRELTAAVDKDTMAYNKVMEAYKMPKDNEEQKE
                     KRKKAIREANLEATEVPYQVMEFSHKALMLAETMLGKCNPNAASDLGVAAVSLMNAVK
                     GAWLNVRINMPGLKDEKKEKYYMENGGNIYKDAEKRSDKIYEVVLKEM"
     gene            4964..5860
                     /gene="ftcD"
                     /locus_tag="PLM92_03315"
     CDS             4964..5860
                     /gene="ftcD"
                     /locus_tag="PLM92_03315"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003005485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HPR24203.1"
                     /translation="MAKIIESIPNISEGRNKEIIEACVDQIRQTPGCTLLDYSSDKSH
                     NRTVITYMGSMEGCEEAGVKLAKKAVELIDLTKHEGEHPRMGCVDVMPFVPIKEATME
                     DCIELSKKVGKRIAQEADLPVFLYERSAAAPHRQNLAKVRKGQFEGMAEKVKDPDWIP
                     DFGGQRIHPTAGVLAVGGRPPLIAYNINLGTNNLSIAEKIGKIIREAGGGLKCVKAMG
                     VMLEDRNIAQVSINMTDFTVTPLHRVTELVRREAARYGVPVIGTEMIGLCPMQALFDA
                     AEYYLQIEDFDASVQVIENHLL"
     gene            5919..>6194
                     /locus_tag="PLM92_03320"
     CDS             5919..>6194
                     /locus_tag="PLM92_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014730318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPR24204.1"
                     /translation="MDPEKPQKKDKKTAVLQGNEACAKAAIQAGCRFFAGYPITPSTE
                     IIEMLSGEMQKAGGVFIQMEDEIGSACSIIGARWGGKKSMTATSGPGY"
BASE COUNT         2094 a         1185 c         1618 g         1297 t
ORIGIN      
        1 gcccggaaag gaagccatgg aaaaagcggg catacccata atcaaactgg aggcaaagga
       61 gggacttgcc cttataaacg gaacacagat aatgacagcc atcggggtca atgtattatg
      121 ggatgccatg aacctgctga aggtttccga catcgctgcg gcaatgacag gagaagcatt
      181 aaatgccgta aagaaggcat atgaccataa agtacatgat ctaagaggac ataaaggcca
      241 aaaggattcc gcagaaaacc tgcttaagct ccttgacggc agcgacaatg cccatgaact
      301 gaacaaaaac aaagttcagg atccctactc attaagatgt gtgccccagg tacacggagc
      361 atcaagagat gcagtgcagt atgtttacga agccgtaagc agagaaataa atgcggtaac
      421 agacaatccg ctgatttttc ccgaagagga cgaagttatt tcggggggga attttcacgg
      481 acaacccatg gctctggcat ttgacttcct taaaaatgcc attgcagaac ttgcggatat
      541 atccgaaaga agaagtgaaa gactgataaa tccacagctt tccgaagggc ttgagccgtt
      601 cctgacaaag cacggaggag tctgttcggg attcatgatc gcccagtatg cggcggcatc
      661 catggtttcg gaaaacaaaa tatacgcaca tccggcatcg gtggattcga tcccttcatc
      721 cggaaatcag gaagaccacg tttctatggg taccacggcg gcaaggactg cagccatgat
      781 ccttgacaat tcacaaaaag ttctcggaat agaattattt gcggcatctc agggaatatg
      841 gctaagagga gagaagggtc tttcaaaagc aacaaaagcg gcatatgatc atatacgcaa
      901 aacagtggag cccgtggatc aggatgtgat tatgcattac gaaataaaaa aattcgatga
      961 aatgataaaa accaatgaaa tagttgaaga agttgaaaaa ataacaggcc ccctgaatta
     1021 ggaaggagga acataatgct tacaaatcag aaaatcgcag aggcaatgac aataaaactt
     1081 gaagacagac ttcccgagta tccaaaattt gttgaaggga aaagacgggc accggacagg
     1141 ggcttcagac ttacaaaaga gcagacgaag atagccctca aaaatgctct tcgttatgtt
     1201 cccgaagaat ttcatgaagt ccttgcgacg gagttcatgg aagaactgaa aacatacgga
     1261 agggtatatg cttaccgcta caggccggag ggcaggatat acggaaagcc cattgaagaa
     1321 tacaaaggga attgtctcga aggaaaagct tttcaggtaa tgatcgataa caatctggat
     1381 tttgaggttg ccctttatcc ttatgaactg gttacctacg gagagaccgg acagccctgt
     1441 cagaactggc tgcagtacag actgataaaa aaatatcttg aagaactcac aagggaccag
     1501 acccttgtaa tagaatcggg acaccctctc ggactgtttc cctcaaaacc cgaagcgccc
     1561 agagtgatct taacaaattc catgatgata ggcattttcg acaaccttga cgactgggag
     1621 atcgcggaag aaatgggagt tgcaaattac ggtcaaatga cagccggcgg atggatgtac
     1681 attggtcccc agggaatagt ccatggcact ttcaatacga tcttgaatgc aggaagaaaa
     1741 tatttgggag tccccgaaca gggagacctg acgggacata cttttgtgtc ctccggactt
     1801 ggaggaatga gcggagcaca gccaaaggcc gcaacaatag ccggagcggt agggatattt
     1861 gccgaggtgg acagatccag gatcgaaaca agacatgaac agggctgggt agatgttata
     1921 ctttccgatc ttgaccaggt gtttgccctg gcgaaagaaa gtgtacagaa aaaagaaagt
     1981 atttcgatag cctactgggg caatatagtg gatcttcttg aggcggccat cgcaaaggat
     2041 tttaagatcg atcttctgag cgatcagaca tcatgtcaca atgcatataa cggaggctac
     2101 tgtcccgtcg gacttacttt ccagaaaaga acggaaatgc tccacagaga cagaaagatg
     2161 ttctgtgaaa tggtggatgc ttccctgcgt agacatttcg aagcaataaa agcacttaca
     2221 aaaaacggga catatttctt tgactatggc aattcattta tgaaagccat gtatgattcc
     2281 gggataaagg aaatatcaaa aaacgggcat gatgaaaaag acggattcat atggccttca
     2341 tatgttgaag atataatggg accgcttctt ttcgattacg gctacggtcc tttcagatgg
     2401 gtatgccttt ccggaaagcc cgaagacctg gatgccacag acaaggcggc aatggactgt
     2461 atagatccca aaaggcgctt ccaggacaga gacaactggg tatggataag agatgccaaa
     2521 aaaaacaaaa tggtggtagg tacccaggcc agaatacttt atcaggatgc cgagggaaga
     2581 atgaatatcg ctctcaaatt caatgaaatg ataaaagaag ggaaaatcgg gccggtgatg
     2641 atcggcagag atcatcatga tgtaagcggc acagattccc ccttcagaga aacagcaaat
     2701 ataaaagacg gcagtaatgt catggctgat atggccgtac agtgttttgc cggaaatgcg
     2761 gcaagaggca tgagtctttg cgcccttcat aacggcggcg gagtaggtat tggaaaagca
     2821 ataaacggcg gcttcggcat gctgcttgac ggcagtaaaa gagttgatga aatactaaga
     2881 tccgcaatga tgtgggatgt aatgggcgga gtagccagaa gaaactgggc acgcaatgaa
     2941 aatgcccttg aagcatcagc aacatataat gaaaaacaaa aaggcaaagc tcatataaca
     3001 ctgccttata tacccgatga gaatttgata gaggaaactg tcggaaagtt tgtgaagtag
     3061 agtatttcgc gggagaaaaa aatgagtaca acacttataa aaaacatagg acttttgcag
     3121 actcccattg gcagccaccc ccacagggga gaaaagcagg gagagaatct taaaataaaa
     3181 aatgcggcag tttttgcaag agacggaata atagaagaaa taacagaaaa tggcagactg
     3241 cccggagcat ttgaaaaaga agatgcggat gagataatcg atgcggaagg gagcctggtg
     3301 acaccgggac tggtggaagg acatactcat cttgttttcg gaggataccg gcagcatgag
     3361 atacctctta agctcaaggg tgcaggatat ctggacatac tgaaagccgg cggcggcata
     3421 cttgatacag tcagaaaaac cagggaagcc gggtttgatg aactgtatga aaaaagcaga
     3481 ggattcctgg atgaaatgat ggaaatggga gttaccacct gtgaggcaaa aagtggatac
     3541 gggcttgatc ttgaaacaga agtaaagatg ctggaggtca taaagaaact taaccgggag
     3601 cacaggatgg atctcgccgc cacatttatg ggagcccatg cagtaccacc cgaatacaaa
     3661 ggaaagcctg atcagtatat tgaaatgatc tgtgaaaaga tacttcctgt ggtaaaagaa
     3721 aaaaaccttg ctgaattcgt tgatgttttt tgtgaggagg gggtttttga tgaagaacag
     3781 agcaggagat atttaagcaa agcaaaagag ttgggatttt ccctgaagat acatgctgat
     3841 gaaatagaag aaatgggggg ctcaaaactg gcaggagaaa taggagcggt ttctgccgaa
     3901 catcttatcg ccatagggcc cgggggactg agcgccatgg caaaggccgg agtaactgcc
     3961 atgtgtctgc cttccacatc tttttatctg ggcaaggatt ttgctccggt gaggaagatg
     4021 atagagatgg ggatacctgt ggcaacggca tcggatttca acccgggctc gtgtccttcc
     4081 ctgaatcttc agttcgttat gaatcttgga tatctgaagt ataaaatgat gcccgaagaa
     4141 atacttacgg cagttaccat aaacccggca tgtgccatag gaaaaggcga tttgtgcggg
     4201 acccttgaaa aggggaaaaa agctgatatg gtgatctgga atgcaaagga tatggaaatg
     4261 ctttgctata gattcggatc caacctggca atggcaacga taaaggaagg agtactgatt
     4321 taatggacct agctgatttg aaagtaaaag aatatctgga tgtgcttcag tcagatgccc
     4381 ccgcacccgg aggcggatcg gtaagtgccc tggccggagc tcagggaatc gcccttatga
     4441 tgatggtggc ggatctgacc tgcggaagag aaaagtattc ggcctttgaa gatgtatgca
     4501 agaaagtaaa aaccgaggca gtaggtattt tcagagaact gaccgcagcc gtagacaaag
     4561 atacgatggc ctacaacaaa gtcatggaag catataagat gccaaaagat aatgaagaac
     4621 aaaaagaaaa aagaaaaaaa gcaataagag aggcaaatct tgaggcaaca gaagtgccct
     4681 atcaggtcat ggagttttca cacaaggcat tgatgcttgc cgaaacgatg ctgggaaaat
     4741 gcaacccgaa tgcagccagt gatctgggag tcgctgcagt cagtcttatg aacgcagtca
     4801 aaggagcctg gctcaatgtg agaataaaca tgcccggcct caaggatgaa aaaaaagaaa
     4861 agtattatat ggaaaacggt ggcaacatat acaaagatgc agaaaagaga tcagataaaa
     4921 tatatgaagt tgttttaaag gaaatgtaaa acaggagatc aaaatggcaa agataattga
     4981 aagtatcccc aatataagtg agggaagaaa caaagaaata attgaggcat gtgtggacca
     5041 gataagacag acaccgggat gcacactcct tgattacagc tccgacaaaa gccacaacag
     5101 aacagttata acatatatgg gcagcatgga agggtgtgag gaagcaggag tgaagctggc
     5161 aaaaaaagcg gtagagctca ttgaccttac aaagcatgaa ggagagcacc ccagaatggg
     5221 ctgtgttgat gtgatgccct tcgtacccat aaaagaggcg accatggaag actgtatcga
     5281 gctttcaaag aaagttggaa agaggatcgc acaagaggca gatctgccgg tatttttata
     5341 tgaaagatcc gcagcagccc cccataggca aaatcttgca aaggttagaa aagggcagtt
     5401 tgaaggcatg gcggaaaaag tgaaagaccc ggactggata ccggatttcg gcgggcagag
     5461 gatccatccc acggcaggag tactggcggt aggaggaaga cctcccctga tcgcatataa
     5521 cataaatctg ggcacaaata atctttccat cgccgaaaaa atcggcaaaa taataaggga
     5581 agccggcgga ggacttaaat gtgtaaaggc tatgggagtc atgctggaag acaggaacat
     5641 cgctcaagtc tccataaata tgacggattt cacagtgacg cctctgcaca gagtaacaga
     5701 actggtgaga agggaagcgg cccgttacgg agttccggta ataggaacag aaatgattgg
     5761 cctttgtccc atgcaggcac tgtttgacgc tgccgagtat tatctgcaga tagaagattt
     5821 tgatgcatcc gttcaggtta tagaaaacca tttgctttaa aaccttttga tgggatatat
     5881 gaggatatat caaaaaaaga aagtataaag aggataaaat ggatcccgaa aaaccacaaa
     5941 aaaaagacaa gaaaactgca gtcctgcagg gaaacgaagc atgtgcaaaa gcagcaatac
     6001 aggcaggatg cagatttttt gcgggttatc ccataacccc ttccacagaa ataatcgaaa
     6061 tgctttccgg agaaatgcag aaagccggag gggttttcat acaaatggag gatgagatcg
     6121 gatccgcatg ttccataata ggtgccagat ggggaggcaa gaaatcaatg accgcaacat
     6181 cgggtcccgg atat
//
LOCUS       DAONHK010000059         6150 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_9849_length_6150_cov_3.983429, whole genome shotgun sequence.
ACCESSION   DAONHK010000059 DAONHK010000000
VERSION     DAONHK010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6150
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9849_length_6150_cov_3.983429"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..159
                     /locus_tag="PLM92_03325"
     CDS             <1..159
                     /locus_tag="PLM92_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009258080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HPR24205.1"
                     /translation="SGSGDHRIVMMAAIAASVCQIPVIISGAQAVNKSYPSFFDDFKK
                     LGGEAIEI"
     gene            149..1342
                     /gene="aroC"
                     /locus_tag="PLM92_03330"
     CDS             149..1342
                     /gene="aroC"
                     /locus_tag="PLM92_03330"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015556657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HPR24206.1"
                     /translation="MRSENKNEFIKTESCFGKNIKVKIFGGSHEDSVGVTITGLPEGT
                     NIDMTELQKFLARRAPGNSPFTTRRKEDDKPVVISGAFTGKTSKLMEDVVSISKEPLT
                     VIIKNTDVRSDDYKSISDIPRPGHADFTARIKYGEKVNMAGGGPFSGRMTAPLCIAGG
                     IAMQILSSGGIEIAAHLSSVNNIYDDPFEPAKVTAGDLKAFRKKNEQLSEFPVINSKA
                     GEKMKDLILDAKNQNDSLGGIVEVCAAGVPAGLGGFMYDGVESTLAPLLFGIPAVKGV
                     EFGTGFASSFLKGSENNDAFYVEKGNIYTLTNHHGGILGGITSGMPLMIRAAFKPTPS
                     ISKEQDSVNMRTLGPEKIITKGRHDPCIAVRAVPVTEAAVAIGLLDLMSEMPEYDMEG
                     NDEFR"
     gene            1329..1616
                     /locus_tag="PLM92_03335"
     CDS             1329..1616
                     /locus_tag="PLM92_03335"
                     /inference="COORDINATES: protein motif:HMM:NF013939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HPR24207.1"
                     /translation="MNLDELRLELDEIDAEMADLFIRRMALAAQIAEYKLTTGTPVQD
                     SSRERKVVRRFEERTGELFAPYVPYFCKGLFDASKAFQHDYMKEKTEGKNG"
     gene            1609..2760
                     /locus_tag="PLM92_03340"
     CDS             1609..2760
                     /locus_tag="PLM92_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008981298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase domain-containing
                     protein"
                     /protein_id="HPR24208.1"
                     /translation="MADMDLENIRKDIDTIDSEITALFKKRMEKTLEVAKYKKANNIP
                     VLSDKREKDILHRISEEIGEPLDGYARLLFNTIFDVSRSYQNNYLAGRSRLSELIDEA
                     LTQTPAIFPKKVVAGCQGIAGSYSQAAAERFFAVPSVMYFNSFEGVFNAVEKGLCQYG
                     ILPIENSSYGSVGAVYDLMQNYNFYIIKSLRLRVSHNLMAKAGTKISDIKEIFSHEQA
                     IGQCGDFIRELKGIKITVCDNTAVAAKMVAESDRSDVAAISSKECIELYGLELLKRNI
                     QTSDNNYTRFICFSKKLEIYPGANKITLVLSLPNIPCSLYHTIAKFATMGINLTKLES
                     RPIPGSDFEFMFYFDLEASVYSRELLDLLGEMQEGPEIFSFLGCYMEAL"
     gene            2757..3992
                     /locus_tag="PLM92_03345"
     CDS             2757..3992
                     /locus_tag="PLM92_03345"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HPR24209.1"
                     /translation="MMSYGLLGGSLSHSYSKEIHEAIGKYEYELFCVSPEEMPDFLRK
                     RNFDGLNVTIPYKKDVMDFCDFIEPAAEKIGSVNTLYFKNGYLWGANTDYSGFIYMVK
                     RSGISVSGKKVLILGSGGASLAVKKALEDLNASEILTASRKKAGGTVSYADLPIDAEI
                     IINATPSGMFPDNTEDPLIDLSLFRKCNGVFDLVYNPFITPLLFAAEDIGIPGSNGFP
                     MLVAQATASAELFTGTGDLHCMNEPLIEKLAAQKENIVLVGMPGCGKTTIGQLLAKKL
                     DRIFTDTDEEISLSDGRRAGDIIIEQGEAYFRELETRICKELGKRNSLVIATGGGAIL
                     KKENIRALKQNGKIIFIERSLDMLPTKGRPLSQKKNSLRELYEDRKPFYEKCSDLSIK
                     NDGLPEESAVKILKLLDML"
     gene            complement(3987..4973)
                     /locus_tag="PLM92_03350"
     CDS             complement(3987..4973)
                     /locus_tag="PLM92_03350"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HPR24210.1"
                     /translation="MLNQKKSKKTFIAAIISVAMIFSMIPASAFAVDSGTAGTPSVIN
                     ISTAEQLKTALGGSDTIHQVEGNSVVLSESVNLTDVNIKVTAGDLKIDLNGHEIIFGG
                     AAFKEGIGLFGGNLTIDDRTGGENGKIATALAEAKLIYVGKGTLTLNAGKISATGTKS
                     YGIYIKNSKSAKVVLQGKENSVAEISGKAYAIKQNGKGKVKIASAAKVTIKKTNYYYK
                     KNLPNRKYTLIKVRYITPTPQGVKLTEKNSRTAKMKWKKVAGADGYVVYKYSSKKGKF
                     VKIRVIRDGLKLSYNCKKLKKGVKYSFRVKAYNVVNGKKILSTYSAKKYIRL"
     gene            complement(5210..>6150)
                     /locus_tag="PLM92_03355"
     CDS             complement(5210..>6150)
                     /locus_tag="PLM92_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPR24211.1"
                     /translation="EVDIENKYEYFGKINVTKKIFLNDEEKQSYSDSFWVALFDESDQ
                     RYGDPKEIEMTGGDANKVVFENLPFGDYVLYETDAQGNKLSGPESGRTPEWIDITYEN
                     NEVSLTEEAPSADVVVNNYFEEKDDPYFGSIEVTKKVTYNGKATGTSLTFYTALFADK
                     EGKEIVTEVKALEMNGKDNVSVIFDKDKNGESLPADTVYYVAETDKDGNPITDPKGYT
                     IQYENQQKVEMDVENDYVGKTTIVNQYEGEDEEFYYYDENGTKITTVKTSATSSETKT
                     GDDINIGLLILLALIGVAGAITPFALRKREKEESDK"
BASE COUNT         1692 a         1282 c         1259 g         1917 t
ORIGIN      
        1 agcggatcag gggatcaccg tatcgttatg atggcggcca tagctgcttc agtatgtcag
       61 atacctgtaa taataagcgg tgcgcaggct gtaaacaaat catatccatc cttctttgat
      121 gatttcaaaa aattgggagg tgaagcaatt gagatctgaa aataaaaacg agtttatcaa
      181 aacagaatcc tgcttcggca agaatataaa agtgaaaatt ttcggcgggt cccacgaaga
      241 ttcggtgggc gttacaataa ccggcctccc cgaaggaaca aacatagata tgacagaact
      301 tcaaaaattt cttgccagaa gagcgccggg aaacagtcct tttaccaccc ggagaaaaga
      361 ggacgataag ccagtggtca tatcgggagc attcaccggt aaaacatcaa aactcatgga
      421 agatgtcgtt tctatttcaa aagaacctct tactgttatt ataaaaaaca ccgatgtcag
      481 atccgatgat tacaaaagca taagtgatat cccaaggccc ggacatgcgg attttactgc
      541 caggatcaaa tacggcgaaa aggtcaacat ggccggcgga ggaccttttt ccggcagaat
      601 gactgcaccc ctttgtatag cagggggaat agctatgcag atcttaagtt ccggaggaat
      661 agaaatagct gcccatttat catccgtaaa caatatatac gatgatcctt ttgagcccgc
      721 caaagtgacc gccggcgacc tgaaagcttt tcgtaaaaaa aacgaacaac tttctgaatt
      781 ccccgttatt aacagtaaag caggagaaaa aatgaaagat cttatactgg acgccaaaaa
      841 tcaaaatgat tctctgggtg gcattgtgga agtctgtgcg gcaggagtcc ctgcaggtct
      901 gggcggtttc atgtacgatg gtgtagaaag cactctcgct cctttattgt ttggtatccc
      961 cgctgtcaaa ggtgtggaat tcggaaccgg ctttgcatct tccttcctaa aaggatcgga
     1021 aaacaacgat gcattttatg tggaaaaagg caatatatat actcttacaa atcatcacgg
     1081 tggaatactg ggaggcataa catccggcat gccccttatg atccgggcag ctttcaaacc
     1141 cacaccttcc ataagcaaag agcaggattc cgtcaacatg agaactcttg ggccggaaaa
     1201 aataataacg aagggcaggc atgatccctg tatagcagtg agggcggtgc ctgtaacaga
     1261 ggcggctgtg gcaataggtc tacttgatct tatgtcggaa atgccagaat atgatatgga
     1321 gggtaacgat gaatttagat gaactgagac ttgagctgga tgagatcgat gccgaaatgg
     1381 cggatctttt tatcagaagg atggctcttg ctgcacaaat agcagaatac aaactcacta
     1441 ccggaactcc tgtgcaggat tcttcccggg aaagaaaggt agtaaggcgt tttgaagaaa
     1501 ggacgggaga gctttttgct ccttatgtac cctatttctg caagggcttg tttgatgcca
     1561 gcaaagcttt tcagcacgat tatatgaaag aaaaaacgga ggggaaaaat ggctgatatg
     1621 gacttggaaa acataagaaa agatattgac accatcgatt cggaaataac cgcacttttt
     1681 aaaaaacgta tggaaaagac tctggaagtg gccaagtata aaaaggcaaa caacatacct
     1741 gttttaagcg acaaaagaga aaaagacata ctgcacagga tatccgaaga aataggagag
     1801 ccgcttgacg ggtatgcaag actgcttttc aataccattt ttgacgtcag cagatcttat
     1861 cagaataatt atctggcagg aagatcccgg ttatctgagc tcattgatga agccttaaca
     1921 caaactcccg ctatattccc gaaaaaagtt gttgcgggtt gtcagggcat agccggatcc
     1981 tattctcagg cggcggcaga aaggtttttt gcagtgccgt ccgtaatgta tttcaacagt
     2041 ttcgaaggtg tttttaatgc ggttgaaaaa ggcctttgtc agtatggcat ccttcccatc
     2101 gaaaacagtt cttacggctc tgttggtgct gtttatgacc ttatgcagaa ctataatttc
     2161 tacataataa aaagccttcg cttaagagtg agccacaatc ttatggcaaa agccggtaca
     2221 aagatttctg atataaaaga gattttttct catgagcagg ctattggcca atgcggtgat
     2281 tttatcagag aacttaaggg catcaaaata accgtatgcg acaatactgc cgttgcggca
     2341 aaaatggtgg ctgaaagcga cagatccgat gttgctgcca tttcttcaaa agagtgcatt
     2401 gaactttacg gtcttgagct gcttaaacgg aatatacaga ccagtgacaa caactacacc
     2461 cgttttatct gtttttcaaa aaagcttgag atctaccccg gtgcaaataa aatcactctt
     2521 gttctttcac ttcctaacat cccctgctct ctttatcata caatagcaaa atttgcaaca
     2581 atgggaataa atctgaccaa actggaatcg aggcctatcc cgggaagcga ctttgaattt
     2641 atgttttatt ttgatctgga agcctcggta tactccaggg aacttctgga tcttctgggt
     2701 gaaatgcagg aggggcctga aatcttttca ttccttggct gctatatgga ggcactatga
     2761 tgagttacgg tcttttgggc ggttctttga gccacagtta ttcaaaagaa atacacgagg
     2821 ccatcggaaa atatgaatat gagcttttct gtgtgtctcc tgaagaaatg ccggactttt
     2881 tgcgcaaaag aaattttgac ggtcttaatg ttaccatccc ctataaaaaa gatgttatgg
     2941 acttctgtga ttttattgaa cctgccgctg aaaagatagg ttctgtaaac actttgtatt
     3001 tcaaaaatgg atacctttgg ggagccaata ccgattattc cggttttatt tatatggtaa
     3061 agagatccgg aatttcagtc tccggtaaaa aagtccttat ccttggcagt ggcggtgcaa
     3121 gtttggcggt aaaaaaagct ttggaagatc ttaatgcctc tgagatatta acggcttcaa
     3181 gaaaaaaagc cggaggcact gtttcttatg ctgatctgcc cattgatgct gagatcataa
     3241 taaatgccac gccttccggc atgtttcccg ataatacgga agatcctctt attgaccttt
     3301 ctcttttcag aaaatgcaac ggcgtttttg accttgtata taatcccttt atcactcccc
     3361 tgctttttgc tgcggaggat attggtatcc ccggatcaaa cggcttccca atgctggtgg
     3421 ctcaagcaac agcatcggcc gagttgttta ccggcacagg cgatcttcat tgtatgaatg
     3481 agcctctcat cgaaaagctt gccgcacaaa aagaaaatat cgttcttgtg ggtatgccgg
     3541 gctgtggaaa aaccaccatt gggcaactgc ttgcaaaaaa gctggataga atttttaccg
     3601 atacggatga agagatatcc ctctccgatg gccgccgtgc gggagatatt attattgagc
     3661 agggagaagc atattttcgt gaacttgaaa cccgtatctg caaagaactg ggcaaaagaa
     3721 attctcttgt tatagccacc ggcggagggg cgatcctcaa aaaagaaaat ataagagctc
     3781 ttaaacagaa tggtaaaata atctttatag aacgctcttt ggatatgctt ccgacgaaag
     3841 gccgtccact ttcacagaaa aaaaattccc tccgtgagct ttatgaagac agaaaacctt
     3901 tctatgaaaa gtgctctgat ctttccataa aaaacgacgg actcccggaa gagtccgccg
     3961 taaaaattct caagcttttg gatatgctat aatcttatgt atttttttgc ggaataggtg
     4021 cttaagattt tcttgccgtt tacaacatta tacgctttta ctctgaaaga atactttact
     4081 ccttttttta gttttttgca gttatacgaa agtttcagtc cgtcacgtat aactcttatc
     4141 tttacaaact tccctttttt tgaagaatac ttatagacaa cataaccgtc tgcaccggca
     4201 acttttttcc atttcatttt tgctgtcctg ctgttttttt ctgtcaactt cactccctgg
     4261 ggagtcggcg ttatgtatct tacttttatc aacgtgtatt ttctatttgg caggttcttt
     4321 ttgtaataat aattcgtttt ctttattgtg actttagctg cagaagctat ctttactttt
     4381 ccttttccat tttgctttat ggcatatgcc ttacctgata tttctgccac actattctct
     4441 tttccctgca gcaccacttt ggccgacttg gagtttttga tgtaaatacc atatgacttc
     4501 gttcccgttg cagaaatctt tcctgcgttg agggtaagtg tacctttgcc aacataaata
     4561 agtttggcct ctgctaaagc tgtggctatt ttaccgtttt ctcctccggt cctgtcatct
     4621 attgtaagat tccctccgaa aaggcctatg ccctctttaa atgcagcccc tccgaaaatg
     4681 atctcatgac cgttaaggtc gatcttcagg tctcctgctg tcacttttat gtttacatca
     4741 gtcagattaa cagactcgct caaaacaaca gaattgcctt ctacctgatg tatggtatca
     4801 ctgcctccca gggcagtctt tagctgctct gccgttgaaa tatttatgac tgaaggagtt
     4861 cctgctgtgc cactgtcaac ggcaaacgca gaagcaggga tcattgaaaa aatcattgca
     4921 actgatatta ttgccgctat gaaagttttt ttgcttttct tttggtttaa cattttttct
     4981 tcccttctca aatcgttttt tcttttggtt aaaacggttc tcttctaata aaagaattat
     5041 tttatattat atcacttttt atttgtgttt tttgtgttgt ttttgtgttt ttataaataa
     5101 aagggaacat gcatctgcgc atgcatgctc ccttgtttat gcctgttctt ctgtagtttt
     5161 tatttcttgt tttttttatc ctgcttcttc aaaatacaat ccggaaactc tatttgtcac
     5221 tttcttcttt ttcccttttc cttaatgcga aaggtgttat tgctccggca actcctataa
     5281 gtgccagaag tatcaacagt cctatattta tatcgtctcc tgtttttgtt tctgaagaag
     5341 tggcgcttgt cttgactgtc gttatttttg ttccattttc atcatagtag taaaactctt
     5401 cgtcttctcc ctcatactga ttgactatcg tagtttttcc cacataatcg ttttccacat
     5461 ccatttctac tttctgctgg ttttcatact ggatggtata gccttttgga tcagttatgg
     5521 ggtttccgtc tttatcggtc tctgccacat agtatactgt gtctgcggga agactttctc
     5581 cgtttttatc cttatcgaat atcactgaaa cattatcttt accgttcatt tcaagagctt
     5641 ttacttcagt aactatttct ttgccttctt tgtccgcaaa cagggcggta tagaatgtca
     5701 ggctggttcc tgtggctttt ccgttatatg tgactttttt tgtcacttct atggaaccga
     5761 aatatggatc gtctttttct tcaaagtagt tatttactac cacatctgca gagggagcct
     5821 cctctgtaag ggatacttca ttattttcat aagttatgtc gatccattcg ggagtacgtc
     5881 cgctttcagg tcccgaaagc ttgtttccct gggcatccgt ttcatataaa acatagtcgc
     5941 caaagggcag gttttcaaag acaactttat ttgcgtctcc gcctgtcatt tctatttctt
     6001 tgggatcgcc gtaacgctga tcgctttcat caaataatgc cacccagaag ctgtcgctgt
     6061 agctctgctt ttcttcatca tttagaaata tcttttttgt tacattgatc ttgccgaaat
     6121 attcatattt attttcaata tcgacttcag
//
LOCUS       DAONHK010000060         6100 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_9958_length_6100_cov_4.715798, whole genome shotgun sequence.
ACCESSION   DAONHK010000060 DAONHK010000000
VERSION     DAONHK010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6100
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9958_length_6100_cov_4.715798"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(83..586)
                     /gene="def"
                     /locus_tag="PLM92_03360"
     CDS             complement(83..586)
                     /gene="def"
                     /locus_tag="PLM92_03360"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010244716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HPR24212.1"
                     /translation="MALRNILKEDDPTLRKRSREVSEINERIIILLDDMKETMTEGAG
                     VGLAAPQVGVLRRVFIASLVNDQEENELKEFIDPEIIEAEGEQEGDEGCLSVPGYIGT
                     VKRPKRIRIKYKDRHGNEKVEDFTDFHAVVVSHEFDHLEGILYTDKAKDIREAVSGAH
                     DEEETDI"
     gene            complement(588..2621)
                     /gene="priA"
                     /locus_tag="PLM92_03365"
     CDS             complement(588..2621)
                     /gene="priA"
                     /locus_tag="PLM92_03365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HPR24213.1"
                     /translation="MKYVDLVIDNKSDSTDTFYTYACENDDIKAGQKVYVPFAKGNKR
                     KGAWVFEVSDKLHEDISKDIKKIKYVEETDKEQPLTEEIVKTCIWMKKRYLCRYVDAI
                     RLFAPSGSASKKGHKRNPHKDTKGEPQPIKELTDQQKKALDPLKEAVHKNKHEIFLLH
                     GITGSGKTEIYMQIIQNCIALGKTAIMLVPEISLTKQIIERFIGRFGNGNIAVLHSRL
                     SPGERFDEWQRIRKGEVRIVIGARSAVFAPVVDIGAIIMDEEHEDTYKSDMTPKYDTL
                     EIAIKRSAYYGGPVILGSATPSVVSYYRSEKGLYRRLVLNERYNKTPLPGVNVADMRL
                     ELKEGNKSVISRALYKDMDETLKAGMKVILFLNRRGYSTFISCRECGHVMQCRECGIS
                     LTYHKEKDRIKCHYCGYEEAVPSVCPSCGSRYIRYFGTGTEKVEEEVKSLFPGHKTAR
                     LDTDTARKKGSMEKILDDFSKGDTDILTGTQIVAKGLDYPNVALVGIISADVSLNIPD
                     FRSAEKAFQLMIQASGRAGRGTEAGKVIIQTYTPENYAVCAAAAQDYRKFYDTEIKLR
                     EYMGYPPFCDLILLVFDSKIRGEAEKGALYWKAGFIALTGKCHEENILPIQQIPSNIS
                     KEGWKCNMLIKCPLGNRKIYMGALEEIKNRLKNDKNKKYNVAVDVNPYSLWRS"
     gene            complement(2631..3641)
                     /gene="asnA"
                     /locus_tag="PLM92_03370"
     CDS             complement(2631..3641)
                     /gene="asnA"
                     /locus_tag="PLM92_03370"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003375467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HPR24214.1"
                     /translation="MSKLTVPEGYTPMIDYMESQRAIKKIKDFFQDELAYGLNLRRVS
                     APLFVSPESGLNDNLNGIERTVDFTLKDMKEKKVEVVQSLAKWKRMALGKYGIEPGHG
                     IYTDMNAIRRDEELDNLHSIYVDQWDWEKVILKEDRNETYLEETVTRLYNALKNLGDF
                     VNRHYPEIKTVMPNEIFFITSQELEDMYPGKTPKEREDAIAEEKKAVFIKKIGGRLRS
                     GKKHDGRAPDYDDWELNGDIILWNDILKTAYEISSMGIRVDEEAMARQLEEDGANERA
                     ELPFHKAILNRQLPYTIGGGIGQSRLCMFFLRKAHIGEVQVSVWPEEMMEECSKNNIF
                     LL"
     gene            complement(3645..4313)
                     /locus_tag="PLM92_03375"
     CDS             complement(3645..4313)
                     /locus_tag="PLM92_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013387409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HPR24215.1"
                     /translation="MKGVKKTEPLSDNIPVVKECTLNYVSVGEIEKNKAKPSLLLHSC
                     CGPCSTAVIERLCPDYDITVFFYNPNITDEKEYIRRKKAQMDFVKRYNDDPAIPCKVG
                     FVEGPYDVRAFYRVAVAFSSEPEGGRRCQQCFALRLEKTAETAKILGFDMFATTLSVS
                     PHKDYDAISHIGKRTALMYNVGFLDKDFKKNNGYTRSIKLSKIYGLYRQDYCGCEYSK
                     KKER"
     gene            4427..5398
                     /locus_tag="PLM92_03380"
     CDS             4427..5398
                     /locus_tag="PLM92_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009644657.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HPR24216.1"
                     /translation="MINNNGSTNGAVPSYSFENHVFNSISSYLKTRFGGKVIKLSIDG
                     GFTCPNRDGTKSVGGCIFCSAGGSGELSGDLPEQIRLLSKKWPKARNYIAYFQAHTST
                     YAPVDILRKKFNQALDHPVVLGLAVATRPDCLSGDVLDFLSEINEKTFLWVELGLQTI
                     HEKTAETINRCYPLSCYDSAISALSQRDIKAVTHLILGLPGENREDMLSSVRYVCRPL
                     HRTDKPVTREEHIFGLKLHLMNVVSGSPMEKLMPEYVPFDSLNEYISLVCDILETIPP
                     EITIHRLTGDVPRKLLIAPNWSYKKRTILNGIMNEMSRRGSWQGCRI"
     gene            complement(5428..6024)
                     /locus_tag="PLM92_03385"
     CDS             complement(5428..6024)
                     /locus_tag="PLM92_03385"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006000461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HPR24217.1"
                     /translation="MAQLFYKYSTMNAGKSIEVIKVAYNYEERGKRVLVMSPAVGDKH
                     ETGVITSRIGIQREAIPIGNETNILELFMKENAREKVDCVLIDECQFLRKHHVQELVE
                     IVDSCDTPVLAFGLKNDFRNELFEGSYYMLVYADKIEEIKTICWCGRKATMVARVVDG
                     KMVKEGKQIMVGGMDMYVPLCRKHYNDGRLGPEMKQKK"
BASE COUNT         1447 a         1462 c         1096 g         2095 t
ORIGIN      
        1 acaaaacata tttcatgccc gctgtttttt attgcttcaa gtgccggtac tgcaaagtaa
       61 ggagtcccca taaagactat tttcatatat ccgtttcctc ctcatcatga gccccggaaa
      121 ccgcctctct tatgtcttta gccttgtccg tatacagtat gccttccaga tgatcaaact
      181 catggcttac gaccacagca tgaaagtcag tgaaatcctc cactttttca tttccatgtc
      241 tgtctttgta ttttatcctt atcctcttgg gtcttttaac ggttcctata tatcccggca
      301 cactaaggca tccctcatcg ccttcctgct ccccttccgc ttctattatt tcgggatcga
      361 tgaattcttt caattcgttt tcttcctgat cgttgacaag ggatgcaatg aatactcttc
      421 tcaggacacc cacctgaggt gccgccagcc ctacccctgc tccttccgtc attgtctctt
      481 tcatgtcatc cagcagtata attattctct catttatttc cgagacttct ctgcttcttt
      541 ttctcagtgt tggatcgtct tctttcaata tgtttcttaa tgccattcta actcctccag
      601 agactgtatg gattcacatc tacagccaca ttgtattttt tgtttttatc gttttttaaa
      661 cggtttttga tttcttcaag agctcccata taaatcttgc ggttgcccaa aggacacttt
      721 atcagcatat tacatttcca gccttctttt gaaatgtttg aaggtatttg ctggatcggt
      781 aatatgtttt cttcatggca tttacctgtc agtgctataa aacctgcttt ccagtataaa
      841 gcaccttttt ctgcttctcc ccggatcttt gaatcaaata ccagaagaat cagatcgcaa
      901 aaaggcgggt aacccatata ttctcttaat tttatttctg tgtcataaaa tttcctgtag
      961 tcctgggctg ctgctgcaca aacagcatag ttttcgggtg tgtatgtctg gatgatcact
     1021 tttcccgctt cggtccctct cccggctctt cccgatgcct gtatcataag ctgaaaagcc
     1081 ttttcggcag atcggaaatc gggtatattc agggaaacgt ctgcggaaat gatccctacc
     1141 agtgccacat ttggatagtc cagtcccttg gccactattt gtgtccctgt caatatgtct
     1201 gtatctcctt tgctaaaatc atccagtatt ttttccatgg aacctttttt tcttgccgtg
     1261 tccgtgtcca gtcttgcagt tttgtgcccg gggaacaagc tcttgacttc ttcctccact
     1321 ttttctgtcc ccgtgccaaa atatcttata tatctgctgc cacaggaagg gcatactgaa
     1381 ggcacggctt cttcgtatcc gcaataatgg catttgatcc tgtctttttc tttatggtat
     1441 gtgagagata taccgcactc acgacattgc atcacatgtc cgcattcccg acatgaaata
     1501 aaagttgaat agcccctcct gttaaggaaa aggatcacct tcatgccggc tttgagagtt
     1561 tcatccatat ctttataaag agctctgctt attacagact tgttaccttc tttgagctcc
     1621 agtctcatat ctgccacatt tactccggga agtggggttt tgttatacct ctcgttcaaa
     1681 accagcctgc gatataaacc tttttcactt ctgtaatatg aaactactga aggcgttgca
     1741 ctgcctaaaa taaccggtcc tccataatat gcggatcttt ttatggctat ctcaagggta
     1801 tcatattttg gagtcatatc cgacttatat gtgtcttcat gctcttcatc catgatgatc
     1861 gcacctatgt ctacaacggg tgcgaaaact gcagaacggg ctcctattac tattcttacc
     1921 tcaccttttc ttattctctg ccattcatcg aatctttctc cgggagatag tctgctgtga
     1981 agcacagcta tattcccgtt accgaacctt cctatgaatc tttctattat ttgttttgta
     2041 agggatattt cagggaccag cattattgct gttttcccca gggctatgca gttttgaatg
     2101 atctgcatat atatttctgt ttttccgctg cccgttatac catgcagaag aaaaatctcg
     2161 tgtttgtttt tgtgaacggc ttccttcaga gggtccaggg cttttttctg ctgatccgta
     2221 agttctttta tgggctgggg ctctcctttt gtatccttat gcgggtttct tttgtggcct
     2281 ttttttgatg cactgccgga aggagcaaaa agcctgatgg catccacata cctgcaaaga
     2341 tatcttttct tcatccatat gcaggtcttt actatttctt ctgtgagggg ctgttctttg
     2401 tcagtctctt ccacatactt gatttttttt atgtctttgc ttatatcttc gtgcagtttg
     2461 tcggatacct caaataccca ggcccctttc cttttgtttc cttttgcaaa cggcacatac
     2521 accttctgcc ctgcctttat atcatcgttt tcacaagcat aggtatagaa agtgtctgta
     2581 ctgtcgcttt tattgtctat taccagatct acatatttca tttcttgttt ttataaaagg
     2641 aaaatattgt ttttggagca ttcttccatc atttcttcgg gccatacaga aacctggact
     2701 tctcctatat gcgccttcct caggaaaaac atgcaaagcc tgctctggcc tatgcctcct
     2761 cctattgtgt aaggcagttg cctgtttagt atcgccttat ggaaaggaag ttctgctctt
     2821 tcgtttgcac catcttcttc cagctgtctt gccatggctt cttcatccac tcttatgccc
     2881 atggaagata tttcatatgc tgtcttaagt atatcgttcc acaggattat atctccgtta
     2941 agttcccagt cgtcgtaatc gggggctctg ccatcgtgtt ttttgccgga tctcaatctg
     3001 ccgccgattt ttttgatgaa taccgctttt ttctcttctg ctatagcatc ctctctttct
     3061 ttaggtgttt tgcccgggta catatcctcc agctcctgac ttgttatgaa aaatatttca
     3121 ttgggcataa ctgttttgat ctcggggtaa tgtctgttta cgaaatctcc gagatttttt
     3181 aatgcattgt ataatctggt caccgtttct tcaaggtatg tctcgtttct gtcttctttc
     3241 agtatgactt tttcccagtc ccactggtca acatatatag agtgcaggtt atcaagttcc
     3301 tcatctcttc ttatggcatt catgtcggta tatataccat gccccggttc aataccgtat
     3361 ttgcccaggg ccattctctt ccacttggcc agtgactgta ccacctccac ttttttttct
     3421 ttcatgtcct tgagagtaaa atcgacagtt ctttcgatgc cgttcagatt gtcgttgagc
     3481 ccgctctccg gactcacaaa aagaggtgca gaaacacgtc ttagatttaa tccgtaagcc
     3541 agttcgtcct ggaaaaaatc ctttattttt tttattgctc tctggctctc catataatcg
     3601 atcattggcg tatatccttc aggtaccgtt aactttgaca tattctacct ctctttcttt
     3661 tttgagtatt cacacccaca gtaatcctgt ctgtataatc cgtatatttt tgaaagtttt
     3721 atgcttcttg tatatccgtt gtttttttta aaatccttat ccaggaatcc cacattatac
     3781 atcagggcag ttctctttcc tatatggctt atggcatcat agtccttgtg aggacttacg
     3841 cttaatgtgg tggcaaacat atcaaaacct aagatttttg cggtttcagc agttttttca
     3901 agcctcagtg caaaacactg ctgacatctt ctgcctcctt caggttcact gctaaaggcc
     3961 accgccaccc ggtaaaaagc tcttacatca tatggtcctt ccacaaaccc cactttacag
     4021 ggtatggccg ggtcatcatt atatcttttt acaaagtcca tctgtgcttt ttttcgtctg
     4081 atatattctt tttcgtcagt tatgttcgga ttgtaaaaaa agaccgttat atcataatcg
     4141 ggacacagcc gctctataac agctgtactg cagggtccgc agcaactatg aagaagcagt
     4201 gacggttttg ctttgttttt ttctatttca cctacactga cgtaattcaa tgtacattct
     4261 ttgaccacag gtatattgtc tgaaagaggc tccgtctttt ttacgccctt catcttaagg
     4321 ttcctttcct gaaacttttc ttcacattat caaaaaaata ttataccata ttatagtaga
     4381 aaaaagcata aatacctgcc actttattac aaaaggatat ctgaaaatga taaataacaa
     4441 tggctccaca aatggcgctg tgccgtcata ttcttttgaa aaccatgttt tcaattcaat
     4501 aagttcttac cttaaaaccc gattcggagg caaagtaata aaactttcca tcgatggcgg
     4561 ttttacctgt cccaacagag acggtaccaa aagtgtcggc ggatgtatat tctgctctgc
     4621 cggaggctcc ggagaattat ccggtgactt gccggagcag atccggcttc tttcaaaaaa
     4681 atggccgaaa gcaaggaact atatagcata ttttcaggcc cacacatcta cctatgcccc
     4741 tgtggacatt cttcgcaaaa agttcaacca ggctcttgat catccggtag tcctgggtct
     4801 ggccgttgcc acaaggcccg actgcctttc cggggatgtc ctggattttc tctctgaaat
     4861 caacgaaaaa acctttcttt gggtggaatt gggacttcag acaattcacg aaaaaacagc
     4921 tgaaacaata aatcgctgtt atcctctttc atgttacgac tctgccatat cagccctttc
     4981 ccaacgggat ataaaggcag tcacacatct tatacttggt cttcccggag aaaacagaga
     5041 agacatgctt tcttccgtaa gatatgtatg caggccttta cacagaacgg acaaaccggt
     5101 cacgagggaa gagcatatat tcggtcttaa acttcatctt atgaatgtgg tatcaggttc
     5161 ccccatggaa aaacttatgc cggaatatgt tccctttgat tccttgaacg aatatatttc
     5221 actggtatgt gacatacttg aaacgattcc tccggagata acaatacaca ggcttacggg
     5281 tgatgtccca agaaagttgc ttattgctcc caactggagc tataaaaaaa gaacgatcct
     5341 taacgggata atgaatgaaa tgtccaggcg aggcagttgg cagggatgtc ggatatagaa
     5401 aactgtcttc tcagaagaca gtttttgtta ttttttttgt ttcatttcgg gtcccagcct
     5461 gccgtcattg tagtgctttc tgcaaagggg cacatacatg tccatgcctc ctaccattat
     5521 ctgcttaccc tccttgacca tttttccgtc gacgactctg gcaaccatgg tagctttacg
     5581 gccacaccag catatggttt ttatttcttc tattttgtct gcatatacaa gcatataata
     5641 tgatccctcg aaaagctcat ttctaaaatc gttttttaat ccgaaggcca gtacgggtgt
     5701 atcgcagctg tccactattt ccaccagttc ctgaacatga tgtttcctta agaactgaca
     5761 ttcatcaatt aggacgcagt caactttttc tctggcattt tctttcatga aaagctcaag
     5821 tatattggtt tcattcccta tgggtatcgc ttctctctga atgcctatcc ttgaagttat
     5881 gacacctgtc tcatgtttgt caccaacagc cggactcatc acaaggaccc ttttgcccct
     5941 ctcttcgtaa ttgtaggcga cttttataac ttctatggat tttcccgcat tcatagtgct
     6001 gtatttgtaa aacagctgtg ccatagtttc ctcctttggg tttaatacgg tctgtaaagt
     6061 taagaatgaa aaatgaggca ccaacccttt gtgatacaat
//
LOCUS       DAONHK010000061         6096 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_9968_length_6096_cov_5.285383, whole genome shotgun sequence.
ACCESSION   DAONHK010000061 DAONHK010000000
VERSION     DAONHK010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6096
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9968_length_6096_cov_5.285383"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1061)
                     /locus_tag="PLM92_03390"
     CDS             complement(<1..1061)
                     /locus_tag="PLM92_03390"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF015284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HPR24218.1"
                     /translation="MPEKPLNEETYEYYISILKNDLSLALGCTEPGAVAYASAEATTV
                     LGRFPDRLDIKCSGNIIKNVKGVIVPNSGGLKGVDSAAVLGTLVGNTDKKLSILENVE
                     EKHIKKASALLKSEFCHVHLKEGEGSLYIEAIAKEKNDTATVVIAGSHTNIVLIQYND
                     KIILDNQTNRKTENGKNKNENSNKNKTNLSINGILDFAKKADIADLKCLLQLQLSVNT
                     AIADEGLRKPYGASVGRTLLKTCGNDIKTRAKAKTAAGSDARMGGSSLPVVINSGSGN
                     QGITVTVPVAEYAAELCADEEKLYRALAISNLVSIHIKNHIGKLSAFCGAVSAACGSG
                     AAITYIDGGGYEEICSTIT"
     gene            1182..1811
                     /locus_tag="PLM92_03395"
     CDS             1182..1811
                     /locus_tag="PLM92_03395"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HPR24219.1"
                     /translation="MMKEKTLKEKVYSLIFDSIIKGKYPQGTILSEKALTEKYKVSKS
                     PVREALVELCKEGVLKSLPRYGYEVVRLTKQDIEDILDFRIILEGGALRESISGIGKE
                     QTDQLEEIDKLCTCEKAKDDFWTHWECNKEFHLKLMSFYSNEFSYNVLKRAMDTLTRA
                     YAQHYWNKWDNISFPRDTKAHIKIIRCIKKKDLEGAIHFLKEDIEHFGI"
     gene            1888..3261
                     /locus_tag="PLM92_03400"
     CDS             1888..3261
                     /locus_tag="PLM92_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017829574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HPR24220.1"
                     /translation="MEYGIICVLPVLTILVIAVSTRRTLFAMVCGLSVAAIILAGKIT
                     GFVGTWFNYLYVSMTNETLQWLVLVIAMFGMLIMLFERSNAVKDFGFWASRFIKTRKQ
                     ALFGTAILGVIIFLDDYLNNLAVGTTMKGMTDRLRIPRTQLAYVVNSVAAPVCVLVPL
                     SSWAVYFSALMKEEGVLVNGSAMSAYIQGIPLTFYAWLAVLVVVLQIIGIIPKMGAIK
                     KDTIRAEETGDVFPLGTDKELIESARTDEMPEGYKPHPWNFLIPLIGMIAITLLTGTD
                     VLMGAFGGVVISFILYLVQRIMKFKELLTACFDGIMSMGFVMVLSVLAFAVQAANTDL
                     DLAGYIISITEPIMKGGFLPVVVFLVCGIYAYATGCFWDLAAIILPLVVPLAHAVGCD
                     PILACAAVFSGAAFGSNTCLYGDGVILCSQGCQIKAIDLMMATLPYALIAGGGSAILY
                     LIFGFIL"
     gene            3280..4362
                     /locus_tag="PLM92_03405"
     CDS             3280..4362
                     /locus_tag="PLM92_03405"
                     /inference="COORDINATES: protein motif:HMM:NF017181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HPR24221.1"
                     /translation="MTKYSSLQISLYNLDNAFGVSGDETEVAGVLCKEMEGLYDEHFE
                     DAMGNQYFVRYGRDKDRKILFSAHMDEIGFIVNYIEDNGLIRFLPVGYHDDRNAVNQD
                     MIVMTDSGKKVMGVTGAKPAHIMTAEDHEKVQKIEDLFLDVGTTSREETLALGINMGD
                     YIGFAREGYVLNGGKYYTGKAVDDRSACAVMVEVLKRLKGEDIEPTVVMVGSVQEEVG
                     LRAGVPIMNNIDPEVMIALDVTLTGDTPGVPIEKCSQKMGEGPGVKYYDWDPDRAMGN
                     NVSRKVTRAIIKTAEKHGIPFQREVFIGGGTDAWQAPMVGKGYLAGGICIPQRYMHTA
                     VGTIHMDDLENTVRLTEHFIREYKGL"
     gene            4488..5336
                     /locus_tag="PLM92_03410"
     CDS             4488..5336
                     /locus_tag="PLM92_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HPR24222.1"
                     /translation="MSKECTHECSSCSSDCSERTKEQTDFREKPHELSNIKNVIAVVS
                     GKGGVGKSMVTSLMAANMKREGYKTAVLDADVTGPSIPKMFGLKEKAAARGEAMLPVK
                     TAGGIPVMSVNLILENDTDPVIWRGPLIAGMVKQLWTDVIWGDIDYMFIDMPPGTGDV
                     PLTVFQSLPVNGIIVVTSPQELVGMIVEKAVRMAEMMNVPVLGIVENMSFFQCPDCGS
                     RHNIFGESRTEETGKRFGIKTISRIPVDPKMASAADNGKIEVLRTDELDEMIKMLIEK
                     DKTDEK"
     gene            5326..6051
                     /gene="larB"
                     /locus_tag="PLM92_03415"
     CDS             5326..6051
                     /gene="larB"
                     /locus_tag="PLM92_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HPR24223.1"
                     /translation="MKNEKLQEMGFANIDAYRAERTGAPEAVFCPGKKPEQIAEIMKT
                     LTGENEDNIIATRATEKDYAAVKKLLPGAVYFEEAGVIAAKRNGSPAGKEKSGSGTVC
                     GSHVQSTVAVVTAGTADIPVAEEAAVVTELLGNHADRIYDVGVAGIHRLFDKIERIRN
                     ADTVIVVAGMEGALASVVGGLVETPVIAVPTSIGYGANLEGLSALLSMLCSCANGVGI
                     VNIDNGYGAACLAYKILRRRENA"
BASE COUNT         1835 a         1171 c         1490 g         1600 t
ORIGIN      
        1 tttgttatcg tagagcatat ttcttcatac cctcctccgt caatataggt tattgcggcc
       61 ccgctgccac atgcggcact gactgcaccg caaaatgcag aaagctttcc tatgtggttt
      121 ttaatatgta tagaaacaag gttactgatc gccagggccc tgtagagttt ttcttcatcc
      181 gcacaaagct cggcagcata ctcggctacc ggaacagtca ccgtaatacc ctgattaccg
      241 ctgcctgaat ttattacaac gggcagagaa cttcctccca ttcgggcatc tgaacctgca
      301 gcagtctttg ctttggctct ggtttttatg tcgttgccac aggtctttag cagtgttctg
      361 cccacggaag caccataggg ctttcttaaa ccttcatctg ctatggccgt atttactgaa
      421 agctgcagtt gcagaagaca tttcagatct gcaatatcag cttttttggc aaaatcaaga
      481 atgccgttta tacttaggtt ggttttattt ttgttactgt tctcgttttt attttttcca
      541 ttttccgttt ttctgtttgt ttgattatca aggatgatct tgtcattgta ctgaatcaga
      601 actatgttgg tatgacttcc ggcaatgact acggttgcag tatcattctt ttcttttgct
      661 atagcctcaa tatacagact gccttcgcct tctttgagat ggacatgaca aaattctgat
      721 ttaagaagtg cagatgcttt tttgatatgc ttctcttcaa cattctcaag aatactgagc
      781 tttttatcgg tgtttcccac cagggtgccg agaactgcgg ccgaatcgac gcctttcagt
      841 cctcctgaat ttgggacaat tacacccttt acgtttttta ttatatttcc gctgcatttt
      901 atatcaagac ggtcagggaa tcttcctaaa acagttgtcg cttctgcaga agcgtaggct
      961 actgccccgg gttccgtaca tcccaaggcc agagaaagat cgtttttcag gatgcttata
     1021 taatactcat aagtctcttc atttaaaggt ttttcgggca tggctgttta tcctccttaa
     1081 gaatactact gataatacca gaaataatat taaatgtcaa aaccatattt acaagcacat
     1141 ccgcatttgt tatacttatc ctgttacccg gaggatcaga aatgatgaag gaaaagactc
     1201 taaaagaaaa agtttattct ctgatattcg acagtatcat aaaaggcaaa tatcctcaag
     1261 ggaccattct cagcgaaaag gcccttacag agaagtataa agtcagtaaa tctcccgtaa
     1321 gggaggcgct tgtggagctc tgcaaagaag gtgtactaaa aagtcttccc cgctacggat
     1381 atgaagtagt gcggcttaca aaacaggata tagaagatat cctggatttc aggatcatac
     1441 ttgagggtgg agctttgagg gagagcatat ccggcatagg caaggaacaa actgatcagc
     1501 tggaagaaat agataaactg tgcacctgtg aaaaagcaaa ggatgatttt tggacacact
     1561 gggagtgcaa caaggaattc catctgaaac tgatgtcttt ttacagcaat gaattctcat
     1621 ataacgtttt gaaaagagca atggataccc ttacgagggc ttatgcacaa cattactgga
     1681 acaaatggga caatatttca tttccaagag atacaaaagc ccatataaaa ataatcaggt
     1741 gcatcaagaa gaaagacctg gagggagcca tacattttct caaagaggac atagaacatt
     1801 ttggcatata acagaaaaag atgatgtggc acatggggtg ccttatcata taagcggttc
     1861 tgccgcaaaa aaaagaaagg agagaaaatg gaatacggaa ttatttgtgt attgccggta
     1921 cttaccattc tggtcatagc tgtcagcacc aggcggacat tgtttgcgat ggtatgcgga
     1981 ctttcagttg ccgcaataat actggctggc aaaataacag gtttcgtggg gacatggttc
     2041 aattatcttt atgtttccat gactaacgag acccttcagt ggctcgttct tgtaatcgcc
     2101 atgttcggta tgctgataat gctttttgag cgttcaaacg cggtaaagga tttcggtttc
     2161 tgggcaagca gattcatcaa gacgagaaaa caggcactgt tcggcactgc catacttggt
     2221 gtaataatct tccttgatga ttatctcaat aacctggctg tgggtaccac aatgaaaggt
     2281 atgacggaca ggctcaggat accaagaaca cagctggctt atgtggtaaa cagtgtggcg
     2341 gcaccagttt gtgtacttgt acctctttct tcatgggcag tatatttttc tgcacttatg
     2401 aaggaggagg gggtcctggt caacggttca gccatgagtg catatattca gggcataccc
     2461 cttacttttt atgcatggct ggcagttttg gttgtggtgc ttcagatcat aggcataata
     2521 cctaagatgg gagcaataaa aaaagacaca ataagagccg aggaaacagg agacgttttc
     2581 ccactgggta ctgacaagga attgattgaa tcggcaagaa cggatgaaat gcccgagggg
     2641 tacaaacctc atccatggaa cttcctgata cctcttatag gtatgatagc catcactctc
     2701 ctgacaggta cagatgtact tatgggagct ttcggaggag tcgtaatatc ctttatactt
     2761 tatcttgttc agaggataat gaaattcaag gaacttctga cagcatgctt cgatgggatc
     2821 atgtccatgg gattcgtaat ggtcctttct gtactggctt ttgctgtgca ggcagccaat
     2881 acagatcttg atcttgcagg atacataata tcgataacag aacccataat gaagggcggg
     2941 ttcctgcctg ttgtagtatt cctggtatgc ggcatatatg cctacgccac aggatgcttc
     3001 tgggatctgg cggcaataat actgcctctg gtcgtacctt tggcacatgc tgtgggatgc
     3061 gatcccatac tggcatgcgc tgcagtattt tccggtgctg cattcggaag caatacctgt
     3121 ctttacggag acggagtgat cctttgttca cagggatgtc agataaaagc catagacctt
     3181 atgatggcaa ctctgcccta tgcccttatt gcgggcggcg gttcagctat cctgtacctt
     3241 atattcggat ttattctata gtatggcaga ggtgataata tgacaaaata tagttcactg
     3301 cagatatcat tatataatct ggacaatgct tttggtgttt caggagacga gaccgaagtg
     3361 gccggtgtgc tttgcaaaga aatggaaggc ctttatgatg agcactttga agatgccatg
     3421 ggcaaccagt attttgtaag atacggcaga gataaagaca gaaagatact tttttcggcc
     3481 catatggatg aaataggctt tatagtcaat tatatcgaag ataacgggct tataaggttt
     3541 ctgcctgtgg gataccatga tgacagaaat gcagtcaatc aggatatgat cgtaatgacc
     3601 gacagcggca aaaaagtcat gggtgttacc ggtgccaaac ctgcccacat aatgactgcc
     3661 gaagaccatg aaaaagttca gaagatcgaa gatcttttcc tggatgtggg tacaaccagc
     3721 cgcgaagaga cccttgctct gggcatcaat atgggtgatt atataggatt tgcacgagaa
     3781 ggttatgtgc tgaacggcgg aaaatattat acaggcaaag cagtagatga cagatccgct
     3841 tgtgcagtta tggtcgaagt tttaaagcgc cttaaaggag aagatatcga gcctacagta
     3901 gtaatggtgg gttctgtgca ggaagaagtg ggcttaagag cgggcgtacc tattatgaac
     3961 aacatagatc ccgaggtgat gatagctctg gatgttacgc tgacaggtga tacaccgggt
     4021 gtccccattg agaaatgttc tcaaaaaatg ggggaaggtc cgggtgtgaa atattatgac
     4081 tgggatcccg acagagctat gggcaacaat gtcagcagaa aagtaacaag ggcaataata
     4141 aaaacagcgg aaaaacatgg gatacccttc cagagagaag tattcatagg aggaggaaca
     4201 gatgcatggc aggcacctat ggtgggcaag ggatatcttg cgggtggtat atgcatcccg
     4261 cagagataca tgcatactgc agtaggaact attcatatgg acgatctgga aaataccgta
     4321 aggcttacag agcatttcat tagagaatat aaaggactgt aagaaaccga aaatatcaac
     4381 aaccaaaggc ctcctttaaa aggaggcctt tataaatatt gaaaaaactc ctgcagggct
     4441 ttataatcaa tctatagtag ttatttaaca ggaaacggag acaagaaatg agcaaagaat
     4501 gtacacatga atgttcatcc tgcagcagtg actgcagtga aaggacaaag gagcagacag
     4561 atttcagaga aaaaccccat gagctttcaa acatcaaaaa tgttatagct gttgtaagcg
     4621 gaaaaggcgg tgtgggaaag agcatggtga catcccttat ggcggcaaat atgaaaagag
     4681 aaggatacaa aaccgctgta ctggatgcag atgtcacagg accttccatc cccaagatgt
     4741 ttggactaaa agaaaaagct gcagcccggg gagaagcgat gctgccggta aagacggcgg
     4801 gaggcatacc tgttatgtct gttaatctta tacttgaaaa tgatacggac cccgtgatat
     4861 ggagaggtcc cctgatagca ggtatggtaa aacagttatg gactgatgtt atctggggag
     4921 atatagatta tatgttcatc gatatgccgc ccggaactgg agatgtgcct cttacagttt
     4981 tccagtcact gccggtaaac ggaataatag ttgtgacttc acctcaggaa ctggtgggaa
     5041 tgatagtcga aaaggccgtt agaatggctg aaatgatgaa tgtccccgta ttaggaatag
     5101 tagagaatat gagttttttc caatgtcccg actgcgggag ccggcataat atattcggag
     5161 aaagcaggac tgaagaaaca ggaaaaagat ttggaataaa aactatcagc agaattcctg
     5221 ttgatcccaa aatggcatcg gcggcagata atggaaagat agaagtgtta cgcaccgatg
     5281 aacttgatga aatgataaaa atgctcattg aaaaggataa aaccgatgaa aaatgaaaaa
     5341 cttcaagaaa tgggcttcgc taacatagac gcatacagag cagaaaggac cggagcgccg
     5401 gaagctgttt tctgccccgg caaaaaaccc gagcagatag cagaaataat gaagaccctt
     5461 acaggagaaa acgaagacaa cataatagca accagggcaa cagaaaagga ttatgccgca
     5521 gtaaaaaagc ttctgccggg agcagtatat tttgaagagg cgggggtaat agcagcaaaa
     5581 agaaacgggt cacccgccgg aaaagaaaaa tcgggatccg gtactgtctg tggaagccat
     5641 gtgcagtcaa cagtagcagt tgtcacagcc gggacagccg atattcctgt tgccgaagag
     5701 gcggcggttg ttacagagct tttggggaat cacgcagaca ggatatatga tgtgggtgtt
     5761 gccggtatac acagattatt tgataagatc gaaagaataa gaaatgctga tacggttata
     5821 gttgtagcgg gtatggaagg agctctggca agtgttgtgg gagggctggt tgaaacacct
     5881 gttatagcag ttcccacaag cataggctac ggtgcaaatc tggaaggact ttccgcattg
     5941 ctttccatgc tttgcagctg tgcgaacgga gttggtatag ttaatatcga caatggctac
     6001 ggtgcagcct gtcttgctta taagatactc agaaggagag aaaatgcatg agctcagcgt
     6061 tatgatggaa gtagtaaaaa cggtggaaga agaggc
//
LOCUS       DAONHK010000062         6022 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_10125_length_6022_cov_5.237305, whole genome shotgun sequence.
ACCESSION   DAONHK010000062 DAONHK010000000
VERSION     DAONHK010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6022
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10125_length_6022_cov_5.237305"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="PLM92_03420"
     CDS             <1..431
                     /locus_tag="PLM92_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009321779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HPR24224.1"
                     /translation="TWPIQNFLLEAYEIGYLQTLVFILVIASLVQLVEVVLKKHVQSL
                     YKSLGIFLPLITTNCAVLAVTIMNIDEGYTYAEALFNALGAGLGFLLAMVLFAGLRQK
                     LEESQMVPAPFKGIPITLITASILSLSFMGFGGIVDGIFA"
     gene            444..1253
                     /locus_tag="PLM92_03425"
     CDS             444..1253
                     /locus_tag="PLM92_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006875879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HPR24225.1"
                     /translation="MDIVIPVSIVALIGLIAGIILTVAAKLMHVSVNEKVEQIRDELP
                     GANCGACGFAGCDDYASALVMKEGISTSACPVGGAYLSIAIARILGVESEAAEPKIAT
                     VLCRGSSDAIRRVMKHDKEWSCRAAIQLYGGQMECRYGCLGLGDCVQACPHGAIKIVN
                     RLAVVDKEACVGCGLCAKACPKNAIEMREESKRIYIACKSLDTGLFTMKACEEGCIGC
                     KRCVDVCRFDAIKVENNLAKIDYDKCKMCGMCVKECITGAIIDLRKKQKKQ"
     gene            complement(1341..1859)
                     /locus_tag="PLM92_03430"
     CDS             complement(1341..1859)
                     /locus_tag="PLM92_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004613777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HPR24226.1"
                     /translation="MKYQNKKITAYRITFIAGMAALALVFSYIEFLIPFFVAVPGIKL
                     GLANLVILTALYKLGFADAAFINIVRVLAAGLLFTGMFGTLYSLAGAFLSIFVMYGLM
                     KTGKFSVVGVSMAGGVFHNLGQLIMAALIVSTPQVFVYLPALMISGILSGAVIGTVAY
                     AIIKRVSFPSFP"
     gene            complement(1843..2208)
                     /locus_tag="PLM92_03435"
     CDS             complement(1843..2208)
                     /locus_tag="PLM92_03435"
                     /inference="COORDINATES: protein motif:HMM:NF018685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HPR24227.1"
                     /translation="MLKKADIILAAIILFAGITASLIIGSISPAKGEKAVIISEGRIF
                     GIYSLYENRTVTVVSNGHVNKILIKGGEAGMISSNCPGKDCIHQGKISKTSESIVCLP
                     NKLIVKIEGKDKNYDEISK"
     gene            2448..5966
                     /gene="nifJ"
                     /locus_tag="PLM92_03440"
     CDS             2448..5966
                     /gene="nifJ"
                     /locus_tag="PLM92_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HPR24228.1"
                     /translation="MSSKKMKTMDGNTAAAYVSYAFTDVAAIYPITPSSPMAEVVDEM
                     AAHGAKNIFGQEVKVVEMQSEAGAAGAVHGSLAAGALTTTYTASQGLLLMIPNMYKIS
                     GELLPGVFHVSARTLSTHALCIFGDQSDVMATRQTGFALLCSSSVQEVMDLAGVAHLS
                     AIKGKVPFLHFFDGFRTSNELQKIETIDYEDFAKMVDMEAVQNFRDNALNPEHPVIRG
                     TAQNPDIFFQQREASQRYYDELPETVAEYMKQISDLTGREYKPFDYVGAPDAENIIIA
                     MGSVCETAEQTVEALMEKGEKVGLVKVRLYRPWAPEYLREVMPKTVKKIAVLDKTKEP
                     GALGDPLYMDIKTMYYNEKNPPEVYGGRYGLGSKDTTPGQIAAAFQNLKQQQPKDQFT
                     IGIKDDLNNSSLTDLEIKTDDEDTVRCKFWGLGSDGTVGANKQAIKIIGDDTDMYAQG
                     YFAYDSKKSGGVTISHLRFGKNRIKSTYLITEADFIACHNQAYVHQYNILKGLKKGGI
                     FVLNCIWNEEELDRELPASMKRYLAGNDIKFYTINATEIAERIGLGNRINMVMQAAFF
                     KLTNVIPINEAVRYLKESIENAYGLKGEKIVKMNQDAVDEGTSSLHEVDVPKKWAEAT
                     DTDGKAKQMPEFIEKILIPMNRQEGEDLPVSAFEDMADGTFPSGTAAYEKRGVAVHVP
                     KWHPENCIQCNQCSFVCPHASIRPVLLNEEEKAKAPDGFETKKAIGKGMEGYEYRIQV
                     SPLDCLGCGNCEDVCPAKTKALTMETIESQGKEAANWTHAMSIPVKDEIMSKTSVKGS
                     QFAMPYLEFSGACAGCGETPYIKVVTQLFGDRMMIGNATGCSSIWGASAPSIPYCKDA
                     NGRGPSWANSLFEDNAEYGLGMAIGVRQIREKIRDDLNAIANYTSKENVIEACRDWIE
                     NMENGTDSRKVSDRLMDVLSGENLCPHCRKLADEVMNKKDFLVKKSVWALGGDGWSYD
                     IGYGGVDHVLASGEDINILVFDTEVYSNTGGQSSKATPTAAVAKFAASGKRTKKKDLG
                     MMAMTYGYVYVAQVGMGADKNQFMKAVIEAEKYKGPSLIICYAPCISHGLKKGMGKTQ
                     ENTKEAVECGYWQLYRYDPQLTEQGKNPFILDSKEPNGKFREFIMGQVRYTSLSKEFP
                     DIAEELFQKTEKDSMERYSKYKKMAE"
BASE COUNT         1906 a         1152 c         1500 g         1464 t
ORIGIN      
        1 tgacctggcc gatacagaat tttcttttgg aagcctatga aatcggctat ctgcagacac
       61 tggtttttat acttgtgatc gcttcgctgg tgcagctggt ggaggtcgtt ttaaagaagc
      121 atgtgcagtc actgtacaaa tctctgggca tttttcttcc tcttataact acaaactgtg
      181 ctgttcttgc ggtgacgata atgaatatag atgagggata tacatatgcc gaggcgcttt
      241 tcaatgccct tggcgcagga ttggggttcc ttctggcgat ggtgcttttt gcgggactcc
      301 ggcaaaagct tgaagagagc caaatggttc cggcaccctt caaagggata cccataaccc
      361 ttataaccgc ttcgatcctg tcactttcat ttatgggatt tggtggcata gttgatggta
      421 tatttgctta gaaaggagac atcatggata tagtaatacc ggtatcgata gtagctttaa
      481 taggcctgat agccggaata atactgacag tagcggcaaa actcatgcat gtttctgtca
      541 atgaaaaagt cgaacagata agggacgaac ttcccggagc caattgcgga gcctgcggat
      601 ttgccggatg cgatgattat gccagtgctt tagtcatgaa agaggggata agtacctcgg
      661 catgccctgt gggcggagct tatctttcta tagcaatagc aagaatactg ggagttgaat
      721 ctgaagctgc ggagccgaag atcgccactg tactttgcag aggcagttcg gatgccataa
      781 gaagagttat gaagcatgat aaagaatgga gctgcagggc ggccatacag ctttatggtg
      841 gacagatgga gtgcagatat ggctgtttgg gtcttggaga ctgtgttcag gcatgtcctc
      901 acggtgcaat caaaatagta aacagactgg cggtggtgga caaagaagca tgtgtcggct
      961 gtggcctttg cgcaaaagca tgtcctaaaa atgcgattga aatgagagaa gaaagcaaga
     1021 ggatatatat cgcctgcaaa tctttggaca cgggtctttt tactatgaaa gcctgtgaag
     1081 aaggatgtat tggctgcaaa aggtgtgttg atgtctgcag gttcgacgca ataaaagttg
     1141 aaaataatct ggcaaaaata gattacgaca aatgtaagat gtgtggcatg tgtgtaaagg
     1201 aatgtataac gggagcgata atagatctaa gaaaaaaaca aaagaagcaa tagaagaaag
     1261 aaacaatata tcaaatcaaa agcaggggct cagacccgga tgcattcata aaaaaccggc
     1321 gtggtaataa aaggatcagt ttacggaaat gaagggaaac tgaccctttt tattattgca
     1381 taagctactg ttcctattac ggcaccgctt agtattccgg aaatcattag agccggcaga
     1441 taaacaaaga cttgaggggt agatactatg agagccgcca taataagctg acccagatta
     1501 tgaaatacac ctcccgccat ggaaacacct actacagaga actttcctgt tttcataaga
     1561 ccgtacatta caaaaatact caagaaagcg ccggccagag aatagagggt gccgaacata
     1621 ccggtgaaaa gaagccctgc agccaaaaca cgaacgatgt ttatgaaagc agcatcggca
     1681 aatcccagct tgtaaagagc tgtcaggatg accagatttg ccagacccag ttttattccc
     1741 ggtacagcga caaaaaaagg tataagaaat tcgatataag aaaaaacaag agccaaagca
     1801 gccattccgg caatgaaggt tattctgtac gctgtaattt ttttattttg atatttcatc
     1861 atagtttttg tcttttcctt cgatcttcac tatcaacttg tttggcagac acactatgct
     1921 ttcggatgtc tttgatattt taccctggtg tatgcagtct ttgcccggac agtttgaact
     1981 tatcatgcct gcctctccgc cctttataag gatcttattc acatgaccgt ttgacacgac
     2041 ggttacagtc ctgttttcat aaaggctgta tattccaaaa atacggcctt cagaaataat
     2101 gacagccttt tctccctttg ccggagatat actgccaatg ataagagaag ccgtgatgcc
     2161 ggcaaataaa attatggctg ccaggataat atccgctttt ttcaacattt gtatcatacc
     2221 gttttattat tgagacttcc aggaaatcag tatatcagaa gaaggcagat actgccacaa
     2281 attgtactga attgtatatt gtattatttt tagtacaata ttgacacttg cataagtatg
     2341 cgataaagta tatctattaa tagtggcggt aaaggcttct ttttgtttat cggaagaaat
     2401 cgctgaatgg taaaagccat ggggtgccgc ccggacagga ggaaaatatg tcaagcaaaa
     2461 aaatgaaaac aatggatggt aatacagcag cagcgtatgt ttcatatgct tttacggatg
     2521 ttgctgcgat atacccgata acaccgtctt cgccaatggc tgaagtagta gatgaaatgg
     2581 cggcgcacgg ggcaaagaat atattcggac aggaagtaaa ggtagttgag atgcagtcgg
     2641 aggccggagc ggcaggcgct gttcacggat ctctggcagc aggagcttta acgaccacat
     2701 atacagcatc acagggtctt ttgcttatga taccaaatat gtacaaaatt tccggcgaac
     2761 ttctgccggg agtttttcat gtttcagcaa ggactctttc cacccatgca ctttgcatat
     2821 tcggtgatca gtccgacgta atggcaacca ggcagacggg atttgccctt ttgtgctctt
     2881 cttcggtaca ggaagtaatg gatctggccg gcgtagctca tctttctgca ataaaaggga
     2941 aagtaccctt ccttcacttt tttgacggat ttcgtacatc aaatgaattg cagaagatag
     3001 agaccattga ttatgaagat tttgcaaaga tggtagacat ggaggcggta cagaatttca
     3061 gagataatgc ccttaatccg gagcatcccg ttataagggg gacagctcag aacccggata
     3121 tatttttcca gcagagagaa gcatcccaga gatactatga tgagcttccc gaaacagttg
     3181 cggagtacat gaagcagata tcggatctga caggaaggga gtacaaacct tttgactatg
     3241 ttggagcgcc ggatgcggaa aatattataa tcgcaatggg cagtgtatgt gaaactgccg
     3301 agcagacagt ggaagcgctg atggaaaagg gagaaaaagt cgggttggtc aaggtgaggc
     3361 tatacagacc ctgggcccct gaatatctaa gggaagtaat gccgaagaca gtaaaaaaga
     3421 tcgctgttct tgacaagaca aaagaaccgg gagctttggg tgatcctctc tatatggata
     3481 ttaaaaccat gtactacaac gagaaaaatc ctcccgaagt ttatggcggc agatacggtc
     3541 ttggatccaa agacacaacg ccgggtcaga tcgccgctgc gtttcagaac ctgaagcagc
     3601 agcagccaaa agatcagttt actataggta tcaaggatga ccttaataac agttctctga
     3661 ctgatctgga aataaaaacg gatgatgaag acactgtaag atgcaagttc tggggactgg
     3721 gttccgatgg aacagttggc gccaacaagc aggccataaa gataatcgga gatgatacag
     3781 atatgtatgc tcaaggatat tttgcctatg attccaaaaa atccggcggc gtgaccattt
     3841 ctcatctgag attcggaaag aacaggataa aatcgacata ccttataaca gaagctgatt
     3901 ttatcgcatg tcacaatcag gcttacgtgc atcagtacaa tatactgaaa ggacttaaaa
     3961 agggcggcat tttcgtttta aactgtatat ggaatgaaga agaactggac agggaactgc
     4021 ctgcttcgat gaagagatat ttagccggaa acgacataaa attctatacg ataaatgcca
     4081 cagagattgc tgaaagaata ggactgggaa acaggataaa catggtcatg caggctgcgt
     4141 tttttaagct gactaatgta ataccaataa atgaggctgt cagatatcta aaggaatcca
     4201 ttgagaacgc ctatggtctc aagggcgaaa agatcgtaaa gatgaatcaa gatgcagtgg
     4261 atgaagggac aagcagtctt catgaagtag atgtgccgaa gaaatgggca gaggctacgg
     4321 atacagacgg gaaggccaag cagatgcctg agttcattga gaaaatactt atacccatga
     4381 acaggcagga gggagaggat ctgcccgtga gtgcttttga agacatggca gacggaactt
     4441 ttccttcggg aacagcagcc tacgaaaaaa gaggagttgc ggtacatgtt cctaaatggc
     4501 atcccgaaaa ctgtatacag tgtaatcagt gctcattcgt ttgcccacat gcttcaataa
     4561 gaccggtact tcttaatgaa gaagaaaaag caaaggcacc tgacggcttt gaaacgaaaa
     4621 aggccatagg aaagggaatg gaaggctacg aatacaggat acaggtaagt cccctggact
     4681 gtctcggatg tggaaactgt gaggatgtgt gtcctgccaa aaccaaagca ctgaccatgg
     4741 aaaccattga aagtcaggga aaagaagcgg caaactggac tcatgccatg agcataccgg
     4801 tgaaagatga aatcatgtca aaaacatccg taaagggaag ccagttcgcc atgccatatc
     4861 tggagttttc cggtgcatgt gcaggatgcg gggaaacgcc ttatataaaa gtggtaacac
     4921 aacttttcgg agacaggatg atgatcggga atgccaccgg ctgttcatct atttggggag
     4981 cctcggcacc ttcgattcct tactgcaagg atgccaacgg cagaggacct tcctgggcaa
     5041 attctctgtt tgaggacaat gccgaatacg gtctgggtat ggccataggt gttcgtcaga
     5101 taagagaaaa gataagagac gatcttaatg caatagcgaa ttatacaagc aaagaaaatg
     5161 tcatcgaagc atgcagagat tggatagaaa acatggaaaa cggcactgac agcagaaagg
     5221 taagcgacag gctgatggat gtgctttccg gagaaaatct ttgtccccac tgcagaaaac
     5281 tggcggatga agtcatgaac aaaaaggatt tccttgtaaa aaaatccgta tgggctcttg
     5341 gcggagacgg ctggtcatac gacataggtt atggaggagt ggaccatgtt ctggcttcgg
     5401 gagaagatat aaacatactg gtattcgata cagaagtata ttcaaacaca ggaggacagt
     5461 catcaaaagc aacgcctact gcggctgtgg caaagtttgc cgcgtccggc aaaaggacca
     5521 aaaagaaaga cctgggtatg atggccatga cttacgggta tgtatatgtg gctcaggtgg
     5581 gcatgggcgc cgataagaat cagttcatga aagctgttat cgaagcagaa aaatacaagg
     5641 gaccttccct tatcatatgc tatgccccgt gtattagtca cggcctgaaa aaaggcatgg
     5701 gaaaaactca ggaaaataca aaagaagcag ttgaatgcgg atactggcag ctgtacagat
     5761 atgatcctca gctcactgaa cagggcaaga acccgtttat acttgacagc aaagagccca
     5821 acggcaagtt cagagaattt attatgggcc aggtgagata cacttctctg tcaaaggagt
     5881 ttccggatat cgcagaagaa ctgttccaga agaccgaaaa agattcaatg gaaagataca
     5941 gcaaatataa gaagatggca gaataggcct ttgtgctgaa tacacaaaaa aacatgatga
     6001 taatgacttg tcaaagtcat gt
//
LOCUS       DAONHK010000063         6006 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_10158_length_6006_cov_4.157284, whole genome shotgun sequence.
ACCESSION   DAONHK010000063 DAONHK010000000
VERSION     DAONHK010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 6006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6006
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10158_length_6006_cov_4.157284"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="PLM92_03445"
     CDS             complement(<1..244)
                     /locus_tag="PLM92_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011989033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HPR24229.1"
                     /translation="MAKELYYGEDARKKLMEGVDKLADTVKITLGPKGRNVLLDKKFG
                     APMITNDGVTIAKEIELDDPVENMGAQLVKEVATKTN"
     gene            complement(263..544)
                     /locus_tag="PLM92_03450"
     CDS             complement(263..544)
                     /locus_tag="PLM92_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009643607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HPR24230.1"
                     /translation="MSFGIKPLGDRVVIKKLEAEEKTQSGIILTGQAKEQPEMAEVVA
                     VGPGTEEEKMELAAGDMVFYSKYAGTEVKYDGEEYTILSQKDVLAKVDK"
     gene            complement(770..1573)
                     /locus_tag="PLM92_03455"
     CDS             complement(770..1573)
                     /locus_tag="PLM92_03455"
                     /inference="COORDINATES: protein motif:HMM:NF037759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPR24231.1"
                     /translation="MQDLRKSKKTKNKKGFTLVEVIIVLVILVILAAILVPSYAAYVD
                     KTKVTRCKVQRAELSQKFLAMYHYDDEIRECTSEDEVIAITGQDVAQYMLDNKYYEGS
                     IICPVHGQPYGFRFTYKNNSPKGEFLCGCVPETDDFSVFEAISKRIAAAYTGWDRSDV
                     IRSVYEEYGGLPEVNGDVLSGTGFEGKTMYWRPYYLGNRTSVVFYGVDISYEDNPQGL
                     WRGKVLSIDGRIYVNTSGNYSIAGFYNYNNVSELLNSSSFRNNFTEKQD"
     gene            complement(1616..2377)
                     /locus_tag="PLM92_03460"
     CDS             complement(1616..2377)
                     /locus_tag="PLM92_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015708907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HPR24232.1"
                     /translation="MLGPIVNAAAIIGCSLAGLLLSKILGESVPDRIQQIIMKALGLA
                     VIYIGISGAMESTHSLVLILSMVIGSIIGEAINIDRAMNRLGEWTEKKLGLSKQEEFE
                     SDDPEFVPEHTGKRRSGSDFTKGFVSATIIFCTGSMAIVGAMQSGLMGDHEMLFAKSI
                     LDGTICILFAYQMGIGVAFSAVPTFIYEGLIAFVSMKAAGFVPADMIVEMGASGSLII
                     AAIGFNFLDIKEIKAANMIPAVFMPCLIMGIQNLF"
     gene            complement(2378..3319)
                     /locus_tag="PLM92_03465"
     CDS             complement(2378..3319)
                     /locus_tag="PLM92_03465"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HPR24233.1"
                     /translation="MKTITIEGVKDFSPDHIFGCGQCFRWERQSDGSYEGIAKGNIAR
                     IYFEAQDPENPFGPGTLTIETTREEEHNGQTGGLQKEQEISFWRGYFDLDRDYGKIKK
                     ELISRDPVMGEAIKSGEGIRILDQEPWETVVSFIISSNNNIPRIKKCINCLAENFGQR
                     AGTCRGKEYYSLPEPRVLAALTKEDLACCRLGYRAGYLIKTAAKIKEDGKEKLEDLRE
                     CSCEDGFSYIKNLSGVGPKVADCILLFSLRKKNRFPVDVWMRKVMHELYGFAEDDIKG
                     MSDFAEETFGEYVGIAQQYLYYHMRKKDEKRKEKKSR"
     gene            complement(3316..4119)
                     /locus_tag="PLM92_03470"
     CDS             complement(3316..4119)
                     /locus_tag="PLM92_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPR24234.1"
                     /translation="MKVYALVGKSGTGKSYQAMSLCKDKDIESIIDDGLFIYNNDSIT
                     GISAKRQPTKVGAIKTALFNTDEHKARVIKKIKEINPQSILVIGTSDTMVQKIVDRLE
                     LPRIEETVYIEEITTENERETAVKQRKGQGKHVVPVPTFQLKRQFSGYFVDPLRIFRG
                     WGTKADFSEKSVVRPTYSYMGDFIISDKVISDIVEKVGSETKGVSDIMRVMTDKRQSG
                     LRITVLTSMHVDSHAAAAAVSLQKKAAEMIEKMTAFNIKHINIEIRGLK"
     gene            4216..4386
                     /locus_tag="PLM92_03475"
     CDS             4216..4386
                     /locus_tag="PLM92_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009525956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPR24235.1"
                     /translation="MAYTISDDCINCGVCKEDCPVDAITEGEEKHVIDAEKCIDCGAC
                     AENCPVDAPKPE"
     gene            complement(4449..5081)
                     /locus_tag="PLM92_03480"
     CDS             complement(4449..5081)
                     /locus_tag="PLM92_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012994669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HPR24236.1"
                     /translation="MKKEARISNAVIRRLPRYRRYLRELQKKGIKKISSNEFSSLIGY
                     TASQIRQDLNNFGGFGQQGYGYNVDDLYNEISAILGLDKKYRMVIAGAGNLGRAIANY
                     TSYYKSGFLVEGIFETNTELIGEKIHDVEVMDYEGMVEFIEKKHIDIGIICTGKNSAQ
                     EVADKMCFAGVRGIWNFAPLDLEVPGHVALEQVHLSDSLHALAYHMNKKE"
     gene            complement(5084..5311)
                     /gene="acpP"
                     /locus_tag="PLM92_03485"
     CDS             complement(5084..5311)
                     /gene="acpP"
                     /locus_tag="PLM92_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013022892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HPR24237.1"
                     /translation="MFDAIKEIIIDQLKVDAADVTMETHLMKDLEADSIDAVEIIMAI
                     EEKYNIEIPDEEAEKFQRVCDLVKFVEEKGK"
     gene            complement(5354..>6006)
                     /locus_tag="PLM92_03490"
     CDS             complement(5354..>6006)
                     /locus_tag="PLM92_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPR24238.1"
                     /translation="LSGGEKARLALLKLMLSGDNVLLLDEPTNHLDIDSREIFEDALA
                     DFPGTVMAISHDRYFLKKIPGRILELTDEGFVEFLGNYDYYAEKKQETGSGRKYLDEL
                     KNKDRKIQGEYRENTPENIREEKPGKREKIPDAAEERKLNKQREAEERRLQREKKAAE
                     QEIEMLEKEIGDLEKQMCGEEAQKNLQTLENMGNRLSRAKEELTKKYQKWLQYHDS"
BASE COUNT         1295 a         1502 c         1112 g         2097 t
ORIGIN      
        1 cgttggtctt tgtggctact tcttttacca gctgtgctcc catgttttca acgggatcat
       61 ccagttctat ttctttggct atggtaacgc cgtcatttgt tatcatgggc gctccgaatt
      121 tcttgtcaag gagtacgttc ctgcccttgg ggcccagtgt gatctttact gtatcggcca
      181 gtttgtcaac accttccata agtttttttc tggcgtcttc gccataatat agttcttttg
      241 ccattgttta tccctacctt tcttatttgt ctactttagc caatacatct ttctggctta
      301 agatcgtgta ttcttcaccg tcgtatttaa cttctgttcc tgcatatttc gaataaaaca
      361 ccatgtcgcc tgccgccagt tccatctttt cttcttctgt tccggggccg actgcaacta
      421 cctctgccat ttcaggctgt tcctttgcct gccctgtcaa aataatgccg ctctgtgttt
      481 tttcttcggc ttcaagtttt ttgattacaa ctctgtctcc taatggtttg attccaaaac
      541 tcattgtctc tacctccacg tatagtatat tgttagcact catcaatttt gagtgctaaa
      601 tctattctac tcgtttcctc ttcgatgtca atactttttt caaaaaaaat ttttgttttt
      661 ttgtggatgg gtcctttttt ttgcatcacg actccaaaaa acaagctgcc acccgtcttt
      721 tttaccttga cggatggcaa ttttgacttt acgaccttga ttttggcatt cagtcctgtt
      781 tttctgtaaa attgttcctg aaagaggagc tgtttaagag ttcagagaca ttattatagt
      841 tgtagaatcc cgctatgctg taattaccgg aggtattcac atatatcctg ccatcgatgc
      901 taaggacctt gcccctccaa agaccctgag gattatcttc ataagagata tcgacaccgt
      961 agaatacgac gctggtcctg tttcccagat aatagggtct ccagtacatg gtttttcctt
     1021 caaatccggt gccggaaagg acatctccgt tcacctcagg aagtccaccg tattcttcgt
     1081 acacagatct tattacatcg ctcctgtccc atcctgtata ggcggcggct attcttttgg
     1141 atatggcttc gaaaactgaa aaatcatctg tctccggtac gcatccgcaa agaaattcgc
     1201 ccttggggct gttgttttta tatgtgaaac ggaatccgta gggttgtccg tggaccggac
     1261 atattatgga tccttcatag tacttgttgt caagcatata ctgtgccaca tcctgccctg
     1321 ttatggcgat cacctcatct tctgaagtgc attctctgat ctcatcatca taatgataca
     1381 tggccaggaa tttctgtgac agctctgccc tctgcacctt gcaccgggtg accttggtct
     1441 tgtccacata agccgcatag gaaggtacca atatggcggc taatataaca aggatcacta
     1501 aaactatgat gacctccact aaggtaaacc cttttttgtt ttttgttttt tttgattttc
     1561 ttagatcttg catattaata tcccctataa atcaatatgt ccctctgccg ttttgctaaa
     1621 acagattttg tatccccatt ataaggcagg gcatgaatac tgccggtatc atgtttgccg
     1681 cctttatttc tttgatatcc aggaaattga atcctatggc ggctattatc aggcttcccg
     1741 aagcccccat ttcaactatc atatcagcag gcacgaatcc ggctgctttc atggatacga
     1801 atgctatgag cccttcgtat ataaaggtgg gcaccgccga aaaagccact cctatgccca
     1861 tctgataggc aaaaagtata catatggtgc cgtcaagtat ggatttggca aaaagcattt
     1921 catggtcacc cataagtccg ctctgcatgg ctcccactat ggccatggat cccgtgcaga
     1981 atattatggt ggccgatacg aatcccttgg tgaaatcgct gccggatctt cttttgccgg
     2041 tatgctccgg aacaaattcc ggatcatcgg attcaaattc ttcctgcttt gaaagcccca
     2101 gctttttttc tgtccattct cccagccggt tcatggccct gtctatattt atcgcttctc
     2161 ctattatgga tccgatcacc atgctgagta taagaacgag actgtgggtg ctttccatgg
     2221 ctcccgatat gcctatatat attactgcca gtccgagggc tttcattatt atctgctgta
     2281 tcctgtccgg gacgctttct cccagtattt ttgaaagcaa aagtcctgcc agtgaacatc
     2341 ctattattgc tgctgcattt acaattggtc ctaacatcta cctgctcttc ttttcctttc
     2401 ttttttcatc cttttttctc atgtgataat aaagatactg ctgtgctatt cccacatatt
     2461 ccccaaaggt ctcttcggcg aaatcgctca ttccttttat gtcgtcttca gcgaagccgt
     2521 aaagctcatg catgactttt ctcatccaca catccacagg gaagcggttt ttcttcctca
     2581 gggaaaaaag cagtatgcag tccgccacct tcggccccac tccggaaagg tttttgatat
     2641 atgaaaagcc gtcttcacag gaacactctc tcagatcttc cagcttttct ttgccgtctt
     2701 cttttatttt tgccgctgtt tttataagat aaccggccct gtagcccagt ctgcagcagg
     2761 ccagatcctc tttcgtaagt gccgccagca cccggggttc cggcaggctg taatattctt
     2821 ttcccctgca tgtgcctgcc ctttggccga aattctccgc caggcagttt atgcattttt
     2881 ttattctggg tatgttattg tttgatgata ttatgaagga taccaccgtt tcccatggct
     2941 cctgatccag tatccttatg ccttctccgc tttttatggc ttctcccatg accggatccc
     3001 ttgatatcag ttcttttttt attttgccgt aatcccggtc aagatcaaaa taccccctcc
     3061 agaaagaaat ctcctgttcc ttttgcaatc caccggtctg accattatgc tcttcttccc
     3121 gggtcgtttc tatggtaagt gttcccgggc cgaaggggtt ttcagggtcc tgcgcctcga
     3181 agtatatccg ggctatattg ccctttgcga tgccctcata gcttccgtca ctttgccttt
     3241 cccatctgaa acactggccg cagccgaata tatggtccgg actgaagtct tttacacctt
     3301 cgatggttat ggttttcatt taagtcctct tatttctata tttatatgct ttatgttgaa
     3361 agccgtcatt ttttctatca tttctgcggc ttttttctgc agtgaaaccg ccgccgccgc
     3421 cgcatgggaa tccacatgca tgctggtcag cacggttatt cgaagaccgc tctgtctttt
     3481 gtctgtcatg acgcgcatga tatcgcttac tccctttgtc tcactgccca ctttttctac
     3541 tatatcactt atgactttat ctgagatgat aaaatctccc atatagctgt atgtgggtct
     3601 gaccacagac ttctcactga aatccgcctt tgttccccat cctctgaata tgcgcagcgg
     3661 gtccacaaaa tatccggaaa attgtctttt taactggaag gtaggtaccg gtaccacgtg
     3721 ctttccctgt ccttttcttt gttttactgc tgtttctctc tcattttcag tggttatctc
     3781 ttcgatatat actgtttctt ctatccgggg aagctccagt ctatctacta ttttctgtac
     3841 catggtatcg gatgttccta taaccagtat gctttggggg tttatctcct ttattttttt
     3901 tatgactctt gccttgtgtt catcggtatt gaaaagggct gtttttatgg ctcccacttt
     3961 tgtgggctgc cttttggctg atatgcccgt tatggaatca ttattatata tgaaaagtcc
     4021 gtcatctatt atgctctcga tatccttgtc cttgcaaaga ctcatggcct gataactttt
     4081 gcctgttccg ctttttccca ccagtgcata aactttcatt ttctcttatc ctttttcttt
     4141 acttattgat ttccctttga tataattact ccagtaaacg ataaaccctt aaattcttac
     4201 aaggaggatt ttaaaatggc gtacacaatt tctgacgatt gtataaactg cggtgtatgt
     4261 aaggaagact gtccggtcga tgccatcact gaaggcgaag aaaaacatgt catagacgca
     4321 gaaaaatgta tcgactgcgg agcatgtgca gagaactgtc ccgttgatgc cccgaaacct
     4381 gagtgatcaa accagtcaga tatagctaat aaccgtttcc caaaagaaac ggttattttt
     4441 attgtcagct attccttttt attcatatga tatgccagtg catgcagact gtcagaaaga
     4501 tgtacctgtt caagggccac atgtccggga acctccaggt ccagcggagc aaagttccag
     4561 atccccctga ccccggcgaa acacatcttg tccgccactt cctgggcact gtttttgccg
     4621 gtgcatatta tgcctatatc tatatgcttt ttttctatga attcaaccat tccctcataa
     4681 tccataactt caacgtcatg gatcttttcg ccgatcagtt ccgtatttgt ttcaaatata
     4741 ccttccacaa gaaaaccgga tttatagtaa ctggtatagt tggctatggc tctccccaga
     4801 ttgccggcgc ctgctattac catcctgtat ttcttgtcca ggcccagtat agcgcttatt
     4861 tcgttataaa ggtcatcaac attatatccg tatccctgct gtccgaatcc gccgaaatta
     4921 ttaagatcct gccttatctg cgaggcagta taccctataa ggctgctgaa ttcattggat
     4981 gatatttttt ttattccttt tttctgcagt tcccggagat atcgcctgta tctcggaagt
     5041 cttcttatta ctgcattgga tatcctggct tcttttttca tgtctatttg cccttttctt
     5101 caacgaactt gaccagatcg catacgcgct ggaatttttc tgcctcctca tcggggatct
     5161 cgatattgta tttctcctca atagccatga ttatttcgac agcgtcaatg gaatccgctt
     5221 ccaggtcctt catcagatga gtttccatag ttacatctgc cgcgtcgact ttcaactgat
     5281 cgattattat ttcttttata gcatcaaaaa tcatagttta cctccataat aatagtttcc
     5341 cgttaattat agtttaggaa tcatggtatt gcaaccattt ttgatatttt tttgtcagtt
     5401 cctcttttgc ccttgaaagc ctgtttccca tgttttcaag ggtttgaagg tttttttgtg
     5461 cttcttcccc gcacatttgt ttttccagat ccccgatctc tttttcgagc atctcaattt
     5521 cctgttctgc agcttttttc tctctttgta gccgtctttc ttctgcctcc ctttgcttgt
     5581 tcagttttct ttcttctgca gcatccggta tcttttccct tttgcccggt ttttcttccc
     5641 ggatgttttc cggggtgttt tctctgtatt ctccctgtat ttttctgtct ttgtttttta
     5701 gttcgtcaag atacttcctg ccggatccgg tctcttgttt tttctctgca taataatcgt
     5761 aattccccag gaactcaacg aatccctcat ctgtcagttc cagtatcctg cccggtatct
     5821 ttttcaggaa atatctgtca tgggatatgg ccattaccgt ccccggaaaa tccgcaagag
     5881 catcctcgaa tatttccctt gaatctatgt caaggtggtt ggtgggctcg tccaacaaaa
     5941 ggacattatc tccggaaagc attagtttga gcagtgccag cctggctttt tcaccgcccg
     6001 aaaggg
//
LOCUS       DAONHK010000064         5917 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_10352_length_5917_cov_4.667690, whole genome shotgun sequence.
ACCESSION   DAONHK010000064 DAONHK010000000
VERSION     DAONHK010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5917
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10352_length_5917_cov_4.667690"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(299..1696)
                     /locus_tag="PLM92_03495"
                     /pseudo
     CDS             complement(299..1696)
                     /locus_tag="PLM92_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514851.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
     gene            2051..4021
                     /gene="uvrB"
                     /locus_tag="PLM92_03500"
     CDS             2051..4021
                     /gene="uvrB"
                     /locus_tag="PLM92_03500"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011917505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HPR24239.1"
                     /translation="MPDFKVVSDFKMTGDQPEAVNKLARGIFDGKKHQTLMGVTGSGK
                     TFTMANLIEKVQRPTLVISHNKTLAAQLHGEFKEFFPENAVEYFVSYYDYYQPEAYVA
                     STDTYIEKDSDINEEIEKLRHSATAALAERRDVVIVASVSCIYGLGSPVDYENQMLSL
                     RPGMEKNRTDILRKLVDIQYDRNDMAFERGTFRVRGDTIDIYPAGAESAVRVELFGDE
                     IENIRQINPVTGEITGERNHISIFPASHYVTTEEKIKEAVLSIEKELEERVIWFKDRG
                     KLIEAQRIEQRTRYDLEMLKEIGRCKGIENYSRHLNGLPAGSRPYTLIDYFPDDFLIL
                     IDESHVMMPQLRAMYAGDRSRKESLVDFGFRLPSALDNRPLKFNEFDEISNQTVYFSA
                     TPAEYEREQSAGISAEQLIRPTGLLDPPVEVIPSEGQIDHLTGEINRVTKDGGRILVT
                     TLTKKMAENLTEYFSGSGIRVRYLHSEVDTLERLEIIRDLRKGEFDVLVGINLLREGL
                     DLPEVKLVAILDADREGFLRTETSLIQTIGRAARNVDGRVIMYADRISPAMKKALEET
                     DRRRKVQEAYNRTKGITPKTIEKAVRAVIEATKQGQDIEYKGKSPVELTKKELKEYVK
                     IMEKEMKQAAADLQFERAAKLRDEIFEYKTRL"
     gene            4021..>5917
                     /gene="uvrA"
                     /locus_tag="PLM92_03505"
     CDS             4021..>5917
                     /gene="uvrA"
                     /locus_tag="PLM92_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005999317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HPR24240.1"
                     /translation="MTKDLYVKGASENNLKSIDVTIPREKMVVLTGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSSYARQFLGQMEKPDVELIEGLSPAISIDQKTTSKNPRSTVGTV
                     TEVYDYLRLMYARIGIPHCPVCGKEISSQTVDQIVDSLMDLGEGSRLTILAPVIAQRK
                     GTHEKVIERIIKEGFIRVRINGDIIRLDEDEIKLEKTHKHTVEIVVDRIIVKEGAESR
                     LAEACETAISNGNGLVVVLVSQKSEGKEAKTKPGETEKVFSTELACPEHGVSIPELEP
                     RMFSFNNPFGACPACKGLGFIQRIDPDLIIEDQNKSITQGCLSNVFASMELAGFYRQM
                     IGALAEEHGTDLDTPYKDLPAKFKDELLYGTGERKLSYYYVSRNTGSRSHRNHPFEGV
                     LNNLERRYMETNSDYIKEKMTKYMSITPCEECGGKRLRPEVLAVTVGGKNIAELSGMS
                     IRESIDFISRLKLSTKEEIIAKQILKEINARLGFLADVGLDYLTLSRAAGSLSGGESQ
                     RIRLATQIGSSLVGVLYILDEPSIGLHQKDNAKLLATLRSLTDIGNTLIVVEHDEETM
                     YAADHIIDIGPGAGVHGGELVAQGTVEDIKKCKESITGQYLNGNKKIDVPEKRRQGNG
                     LFIEIKGA"
BASE COUNT         1821 a         1189 c         1383 g         1524 t
ORIGIN      
        1 ctgtgtcgtt attctacatc agcttggagg tttacacaat ctttgaaacg gtctcgatta
       61 tataaacatt tttatattgt caaccgagaa atttgctgcg cagctttttt gccagggagt
      121 tttcgttttt cccatgatct ttattttttt gatttccctc tttttctaca tggttttgtt
      181 tggagttcgc ggcctttgcc ttttcttccc tgactttttc ttctctggct ttttcttttc
      241 cttctttggc ttccttgcgg atctctttgc gagcctcctt ttttacttcc tttgcatcct
      301 ttctttctga aatcgggatg tagcttttat gtccttccac attgacataa gcaggacgca
      361 taagacgctt gcctgctatc agttcttcca tcctgtgggc agaccagccg gaaagtctgg
      421 ctgtggcaaa aagaggtgta gctatatcta tgggtatatc gagggcattg taaacaaagc
      481 ccgaataaag atccacattg gcagatatgg ccttttcatt tcccgttact tctgcaaaaa
      541 gctttggagc aatagattct atatgctcaa aaagctcaaa ctcatcaagt atgcctttgt
      601 ttgcagccag ttcctttgcc atcttcttga gcagtaccgc tcttggatcg gaaagtgtgt
      661 atatggcatg gcccatacca tagataagac cgcttccgtc attggcctgt ccgcgtagga
      721 ccctgagaag atatgcatcg acatcttttt tgtttgtgat atcccttacg tgtttcttaa
      781 ggtcatcaat cattcctatt acggctatgt tagcaccgcc gtgtctggga cctttcagag
      841 aaccgatggc ggcagaaatg gtggcatatg tatcggtacc ggaagaagaa acaaggtgtg
      901 tggtgaagga tgaattgttt cctcctccgt gctcggcatg gagcatgaga gaaagatcca
      961 gtactttggc ttccagctct gtatattcgc ccattggacg gatcatgcga agaatatttt
     1021 ctgccgttga aagttccggc agaggattat gcagatgaag gctggccttg tcaaagaaag
     1081 aggcttttgc ctgataagca taggatatca gagccgggaa ataacccata aggtctatgg
     1141 actgtctcaa aacatttatt acagaagtat catcgggatt ctcatcataa cagtaaagag
     1201 ccagtacact tctggccagc ttgttcatga tgcttctgga aggagtatta agtatcatat
     1261 cccgcgcaaa gccctggggc aggtctcgtt tctccgaaag caaacttgtg aattcagaca
     1321 gttctttttt agtgggcaac tggccaaaaa gcagcaaata agttacttct tcaaaaccaa
     1381 acctgttttc attaagacag tttgttacca gttcttctat atcatatccc ctgtaataca
     1441 gtttgccttc caaaggtatt ttgttttttt cctcatcgat acggtatcct acaacttcgc
     1501 ttatttttgt caggccgact atcaccccag tcccgttggc atttcttaat cctcgcttaa
     1561 cgttgtattt agtgtataaa tcagcggcga tctcattttt gaccgctaat tcttttgaca
     1621 ttttttctaa ataagatttt gggatacccg aagccgtttt tttgccggct gttatttttc
     1681 ttccaaagtt caacatatct tcccctccta acttccggtt tcctgtgcaa tggtagcatt
     1741 ataccataat aaagtgatat atagcaataa agttatacta aatatattct gcataatgta
     1801 aaaaaaggcc ggcttatcag gacttttttg tgttcatttc cgtctgccgg atatctcacc
     1861 agaacttcca agtcgccttc gtcatctttc actatttcta ttatgataac aagtgttttt
     1921 ttcgattttg attattccaa agaaagaaaa acagcgtcgg aaaaaccgaa catctgtttg
     1981 caataagttg tttaatgtgt tataataagc gctgtaaatt ctgaaaagaa acaaaaagga
     2041 aagcatacaa atgccggatt tcaaagttgt atctgatttc aaaatgacag gggatcagcc
     2101 cgaggccgta aacaaactgg cccggggtat ttttgacggt aaaaagcacc agaccctgat
     2161 gggagttaca ggttcaggca agacctttac aatggcaaac cttattgaaa aagtacaacg
     2221 tcccacactg gtcatctccc acaacaagac gctggcggct cagcttcacg gcgagttcaa
     2281 agagtttttc ccggagaatg cggttgaata ttttgtatca tattatgatt attatcagcc
     2341 ggaggcatat gttgcatcca ctgatacata tatcgaaaag gattctgata taaacgaaga
     2401 aatagaaaag ctgagacatt cggccactgc agcactggct gagcggagag atgtggtgat
     2461 agtagccagt gtttcatgta tatacggact gggaagccct gttgattatg aaaaccagat
     2521 gctttcgtta agaccgggca tggaaaaaaa cagaacggat atattaagga aactggtgga
     2581 tatacagtat gacaggaatg acatggcttt tgagagagga acttttcgtg taaggggaga
     2641 caccattgat atctatcccg ccggtgccga aagtgctgtc cgggtagagc ttttcggaga
     2701 tgaaatagaa aacataagac agataaatcc tgttacggga gaaataacgg gagaacggaa
     2761 ccatatatcc attttcccgg cttcccatta tgtgacaaca gaagaaaaaa taaaagaagc
     2821 tgttttgtcc attgaaaagg aacttgaaga aagagtgatc tggttcaaag acaggggaaa
     2881 actgattgag gcccagagga tagaacagcg gaccagatat gatctggaaa tgctcaagga
     2941 gatcggaaga tgcaaaggta tagaaaacta ttcccggcat ctgaacggat tgccggcagg
     3001 gagcaggcca tatacactta tagactattt ccccgatgac tttctgatac ttatagatga
     3061 atcccatgtg atgatgcctc aattaagggc catgtatgca ggagaccgct caaggaaaga
     3121 gtccctcgtt gactttggtt tcaggctgcc ctcggcactg gataacagac cgcttaaatt
     3181 taacgaattt gatgaaatat ccaatcagac ggtatatttc agtgccactc cggcagaata
     3241 cgaaagagaa caaagcgcag gcatatctgc ggaacagctc ataaggccca ccgggcttct
     3301 tgatcctcct gttgaagtga tcccttcgga agggcaaata gatcatctga ccggagaaat
     3361 aaacagggta acaaaagacg gaggaaggat ccttgtcacc accctgacca agaaaatggc
     3421 ggagaatctc acggaatact tttccggcag tggcataagg gtaagatatc ttcattccga
     3481 ggtcgatacc cttgagagac tggaaataat aagagatctc aggaaaggag agttcgatgt
     3541 acttgtgggg ataaaccttc tcagagaagg tcttgatctg ccggaggtaa agctggtggc
     3601 catactggat gcagaccggg agggttttct cagaacagaa acatctctta tacagaccat
     3661 aggcagagca gcaagaaatg ttgacggaag agtcataatg tatgcggatc gtataagtcc
     3721 cgccatgaaa aaggctctgg aagaaacaga cagaagaaga aaggtacagg aagcatacaa
     3781 caggacaaaa ggcataacac caaaaacaat agaaaaggca gtaagagcgg tcattgaagc
     3841 cactaaacag ggacaggaca ttgaatacaa gggtaaatca cctgtggaac taaccaaaaa
     3901 agagctcaag gaatatgtaa aaataatgga aaaagaaatg aaacaggcag ctgccgatct
     3961 gcagttcgaa agagccgcaa agctgagaga tgaaattttt gaatacaaga cgaggttatg
     4021 atgacaaaag atctttatgt aaaaggtgca tcggagaaca atcttaaaag tatagatgtg
     4081 acaataccca gagaaaaaat ggtggtgctg acaggacttt ccggcagcgg caaatcatcg
     4141 ctggcttttg ataccatata tgcggaagga cagaggcgct atgttgaaag cctttcatcc
     4201 tatgcacgcc agtttctggg acagatggaa aaacccgatg tggaactcat tgaagggctt
     4261 tctccggcca tatccataga ccagaaaacc accagcaaga atccccgttc cacagtggga
     4321 acggtaacag aggtatatga ttatctaagg ctgatgtatg ccaggatagg tataccccac
     4381 tgtcccgtat gcggcaagga gataagcagc cagactgttg atcagatagt ggacagtctg
     4441 atggatcttg gtgagggatc cagacttact atactggctc cggtcattgc tcagcgcaaa
     4501 ggcacccatg aaaaggtaat agagcgaata ataaaagaag gttttataag agtaaggata
     4561 aacggtgata tcattcgcct tgacgaggat gaaataaaac ttgaaaaaac tcataaacat
     4621 actgttgaaa tagttgtgga ccgcataata gtcaaggaag gggcagaaag caggcttgca
     4681 gaagcttgtg aaacagccat atcaaacggc aacggacttg tagtcgtgct cgtttctcaa
     4741 aaatcagaag gcaaagaagc aaaaacaaag ccgggagaaa cagaaaaagt cttcagcaca
     4801 gagctggcat gtcctgaaca cggggtaagt ataccggaac tggagcccag gatgttttca
     4861 ttcaataatc ccttcggcgc atgtccggca tgcaaaggac ttggttttat ccagagaatt
     4921 gacccggatc ttattattga agatcaaaac aaaagcataa cccagggctg tttgagcaat
     4981 gtttttgcat ccatggaatt ggcaggattt tacagacaga tgataggggc actggccgag
     5041 gaacacggaa cggaccttga tactccatac aaagatctgc ctgcaaaatt caaggatgaa
     5101 ttgctttacg gaaccggaga aagaaagctt tcatattatt acgtgagcag aaataccgga
     5161 tcgagatccc acaggaacca tccctttgag ggggtactga ataatctgga aagacgttac
     5221 atggagacaa attcagatta cataaaagaa aagatgacaa agtatatgag cataacccca
     5281 tgtgaagaat gcggagggaa acgcctgcgg ccggaagttc tggctgttac cgtgggaggg
     5341 aagaatatcg cagagctttc cggtatgtcc ataagggagt caatagattt cataagccgg
     5401 ctcaagcttt caacaaaaga agaaatcatc gcaaaacaga tattgaagga gataaatgca
     5461 agactgggat ttctggcgga tgtgggcctg gattatttga ctttgtcccg tgcagccggg
     5521 tcactttccg gaggagaatc ccaaaggata agactggcca cccagatagg ttccagcctt
     5581 gtgggagtcc tttacatact tgatgaaccc agcataggcc ttcaccagaa agacaatgca
     5641 aaactgctgg caactctaag gagtcttaca gatataggca atactctcat tgttgttgaa
     5701 catgatgaag aaaccatgta tgcggcggat catattatag atataggccc cggagcaggg
     5761 gtacacggcg gagaactggt ggcgcaggga acggtggaag acataaagaa atgcaaagaa
     5821 tctattacag gacagtatct taacggaaac aaaaaaatag atgtgcccga aaaacgccgg
     5881 cagggaaacg gactttttat agaaataaag ggagccg
//
LOCUS       DAONHK010000065         5855 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_10499_length_5855_cov_4.573966, whole genome shotgun sequence.
ACCESSION   DAONHK010000065 DAONHK010000000
VERSION     DAONHK010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5855
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10499_length_5855_cov_4.573966"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            222..1178
                     /locus_tag="PLM92_03510"
     CDS             222..1178
                     /locus_tag="PLM92_03510"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPR24241.1"
                     /translation="MEAIKGGILFCYMLALFLGVGALTMALLAGKKNSNKYNKALTVF
                     LSGMLVMSLYDMFIYYMDYQIGPFDDFLALRIGSSIIAILFYLWLRLECEITDGTRLS
                     WFNKMAGIYVMLYALLWVLLGTALSYDYIYMIRWLLLVTDIVLLLILLVGSVAYMGKA
                     LLTGKSRGSVYYMIVVTSMLAWNYSSFFWGEASRYWGNGTFVREPLDLTIIFWFIVNT
                     STLIFVYKKNFIEAYGNKNTNEGYFDLNERMEEIAKEYEMTNREKDLCRLVYEGKSNR
                     QIAQALFISESTVKTHVYNIFRKMGVKNRVGVARVVRGENRE"
     gene            complement(1266..1586)
                     /locus_tag="PLM92_03515"
     CDS             complement(1266..1586)
                     /locus_tag="PLM92_03515"
                     /inference="COORDINATES: protein motif:HMM:NF017007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HPR24242.1"
                     /translation="MTNPGKIQNYIGKGHKDEIETLKNQITESGIDKLKLKNPKMILI
                     LSIFLGFLGIDRLYQGGIKMFLCKIAMVCLTFGTWWLTDIYFAKHCAEEDNYNKILVM
                     ANDL"
     gene            1810..2571
                     /gene="surE"
                     /locus_tag="PLM92_03520"
     CDS             1810..2571
                     /gene="surE"
                     /locus_tag="PLM92_03520"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0008254 - 3'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HPR24243.1"
                     /translation="MNILVVNDDGIGLRGIKELAGVLSDAANIYVSAPHEQRSASGHS
                     ISVGVPVYIEEVGFENAKMALEVTGTPADCVKLGLSYLEDKGINIDMVYSGINHGGNL
                     GTDTLYSGTVSAAMEGMLCGKPSVAVSVNNHYPKHFEYACGLALKTFHKAKDMLDNKT
                     VLNINVPDLPKEKIKGVRYTRLGIREYEEWFRPKKNESGAMEYWYYGEPVVYDNKDCS
                     IDVIAMQEAWASITPLKWDLTNHDLIDEIEKWEII"
     gene            2571..3113
                     /locus_tag="PLM92_03525"
     CDS             2571..3113
                     /locus_tag="PLM92_03525"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HPR24244.1"
                     /translation="MRIVKEKTMAVAIDFQEKLMRVMKDPKGLEKTVAAFMEGIKVLG
                     IPLIVTQQYTKGLGETTQAIKETMAGMIPVEKTSFSALKTGEFEKKIKDTGLDTVLLC
                     GIETHICVMQTAMDLLERGYKVFVIADCTASRNEKDKNCGLRRMERAGAVITTYEAVL
                     FEILEDAKNEGFKDISRIVK"
     gene            3281..4459
                     /locus_tag="PLM92_03530"
     CDS             3281..4459
                     /locus_tag="PLM92_03530"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HPR24245.1"
                     /translation="MREVVIVSAARTPIGSFGGTLKNVAARTLGTIAVKGAIDRAGIK
                     PEDVDEVIMGCVLQGGLGQNIARQISIDAGIPKEVPSMTINKVCGSGLRAVELAAQII
                     KANDAEIIVAGGAESMSMAPYVTRKARWGARMGDDKLLDMMVYDGLTDVFGQYHMGIT
                     AENICEQWGLTREELDEFAAASQQKTMAAIKAGRFKDEIIPVEIPQRKGDPIVFDTDE
                     FPKDGVTKESIGKLRPAFKKDGIVTAANASGINDSGAAVVVMSKDKADELGLESMCTV
                     LSYASAGVDPSIMGIGPVPATKKALEKAGLTVDDLDLIEANEAFAAQSVAVGKELGFD
                     KEKLNVNGGAIALGHPIGSSGCRILITLIYEMQKRDSKKGLATLCIGGGQGTAMIVER
                     "
     gene            complement(4590..5396)
                     /locus_tag="PLM92_03535"
     CDS             complement(4590..5396)
                     /locus_tag="PLM92_03535"
                     /inference="COORDINATES: protein motif:HMM:NF024919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HPR24246.1"
                     /translation="MKTKKLLIVLLTLVMVFAFAACGGDDETAAFEKHAIPYPEGYDT
                     TTTGASSYDGGGDHFDGGYFVPLDVYNLESTDSLTVIPEFETYQQTTEYSCGPCSALM
                     VLNHYDMYDEKKYDELSIAEMSDTSDETGVGPDGIAKFFEEIGWDVERSPEDGSATFS
                     WDTSDTADQDFIKWVKKNLKAETPIMVDWIDWSGHWQVIIGYDDMGTPDSFGDDVVIL
                     ADPYDTSDNYQDGYYTYGAERFFYMWHESSSLTGEPAEQPFVIATPPEEE"
     gene            complement(5484..>5855)
                     /locus_tag="PLM92_03540"
     CDS             complement(5484..>5855)
                     /locus_tag="PLM92_03540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPR24247.1"
                     /translation="SSEPAVWARGYFERIKGFYGNRIKISASEKDFDINHDWTYGAYN
                     NPVSEVREAIYYMARREAIILDPCYTGKTFNGILKIIDSGLIDKNQTVIFIHTGGNPG
                     INSLPHRAEMESDLKADFKIF"
BASE COUNT         1900 a         1038 c         1348 g         1569 t
ORIGIN      
        1 actggcacaa agagaagctg acaaagaagc tgctcttaaa accctggagg cttaaacggg
       61 tcataaaagt cccgcttaaa taataataag tgaatcccga aaagtccgat agtattcgga
      121 ctttttgggt tgataaacag aaattcggca taaaacgaaa agacaatctt gcaagttttt
      181 caaaaaagtg tatacttgtt tacatctttc ggaggtaaaa aatggaggcc ataaagggag
      241 gtatcctttt ctgttatatg ctggcattgt tcctgggtgt tggggctctt acaatggcac
      301 tgctggcagg aaagaaaaac tcaaataaat ataataaggc attgactgtg ttcctgtcgg
      361 gaatgcttgt tatgagtctt tatgatatgt ttatatatta tatggattat cagataggac
      421 cctttgatga ttttcttgca cttaggatag gaagcagtat aatcgccata ttgttttatc
      481 tctggttaag gctcgagtgt gagatcaccg atggtacccg cctttcatgg tttaataaga
      541 tggccgggat atatgtcatg ctttatgcac tgctttgggt gcttttgggt actgctcttt
      601 cttacgatta tatttatatg ataagatggc ttctgctggt tacggatatc gttttgcttc
      661 ttattctgct ggtaggcagt gtggcatata tgggcaaggc acttctgacg ggaaaaagcc
      721 ggggaagcgt ttactatatg atagtggtaa catcgatgct cgcatggaac tattcttcat
      781 ttttctgggg cgaagcatcc agatactggg gcaacggaac atttgtaaga gagcctcttg
      841 acctaacaat aatattctgg ttcatagtta atacatctac acttatattc gtttacaaga
      901 aaaactttat tgaagcatat ggtaataaga atacaaatga aggatatttt gatctgaatg
      961 aaagaatgga agagatagca aaagaatatg aaatgaccaa cagagaaaaa gatctatgca
     1021 gacttgtata tgaaggcaaa agcaacaggc agatcgccca ggcacttttc atttccgaaa
     1081 gtacggtgaa aacccatgtt tacaatatat tcaggaagat gggagtgaaa aacagagtgg
     1141 gcgtggcaag agttgtaaga ggagaaaacc gggaatagaa atatcgccgt ctgagataaa
     1201 agataaacta aaaagccgtc caaaaggtat gtgacccggg acggctttcg tatgcgtttt
     1261 ttgatctaaa gatcattcgc cataacaaga attttattat agttgtcttc ttcggcacaa
     1321 tgcttcgcga aatatatatc tgtcagccac catgtcccaa aagtcaggca gaccattgca
     1381 attttacata gaaacatttt gatacccccc tgatatagtc ggtctatgcc taaaaatcca
     1441 agaaagatgg aaagtataag gatcattttg ggatttttga gttttaactt gtcgattcct
     1501 gattcagtaa tttgattttt caaagtttcg atttcatctt tgtgtccttt tccgatataa
     1561 ttttgtattt tgcccgggtt tgtcatgtga aagcctcctt tatgattatc agtatcgttt
     1621 tgatttcgtg aaaattatat cagaagcaaa taaaacggta tgtagagaga tagtattaaa
     1681 gaagacagaa aaacaaatga acaaataata ctttcgtact aaaataatca taggctgttt
     1741 tttttatgat ataatcaaaa tgcaaacaaa aatggaaatg gtaggatgtc aaaaacaagg
     1801 aggactttaa tgaatatact ggtggtaaat gatgacggaa taggattaag gggcatcaag
     1861 gaactggcgg gagtgctttc cgatgctgcg aatatatatg tatccgcacc acatgaacaa
     1921 aggagtgcca gcggacacag cataagtgta ggggttcccg tctacattga agaagtggga
     1981 tttgaaaatg cgaaaatggc attggaagta acgggaacac cggctgactg tgtaaaactc
     2041 ggtttgagtt atctggaaga taaaggcata aatattgata tggtctattc cggaataaat
     2101 catggaggaa atctgggaac agatactctt tattcgggaa cggtatcggc tgccatggaa
     2161 ggaatgcttt gcggaaaacc atcagtggca gtctctgtaa acaaccatta tccaaaacac
     2221 tttgagtatg catgcggact ggcccttaaa acttttcata aagcaaaaga catgctggat
     2281 aacaagacag tgctgaatat aaatgtgccg gatctgccca aagaaaaaat aaaaggagtg
     2341 agatatacaa ggcttggcat aagagaatat gaggaatggt tccgccccaa aaaaaatgaa
     2401 agtggtgcaa tggagtactg gtattatggt gaacccgtag tatatgacaa taaggactgt
     2461 tccatagatg ttattgccat gcaggaggca tgggccagca taacgcccct caaatgggat
     2521 ctgacaaacc atgatcttat agatgaaata gaaaaatggg agataatata atgagaatag
     2581 taaaagaaaa aacaatggcg gtcgcaatag attttcaaga aaaacttatg cgggtcatga
     2641 aagacccaaa agggcttgaa aaaacagtgg ccgcattcat ggagggcatc aaagtgttgg
     2701 gaataccgct catagtgact cagcagtaca caaaaggtct gggagaaaca acccaagcta
     2761 tcaaagagac aatggcagga atgatacctg tcgaaaaaac atctttcagc gcacttaaga
     2821 ccggggaatt tgagaaaaaa ataaaagata ccggtcttga cacagtactg ctttgcggga
     2881 tagaaactca tatatgtgta atgcagacgg ccatggatct tcttgaaaga gggtataaag
     2941 tttttgttat agcagactgc acagcatcaa ggaatgaaaa agataagaat tgcggcctcc
     3001 ggcgcatgga aagggcggga gcagtaataa cgacctatga agcagtactt tttgaaattc
     3061 ttgaagacgc aaaaaatgaa ggcttcaaag atatatcccg tattgtcaaa tgagaaatag
     3121 aatgtggata aaaagacccg cataaacaag aatgttaact aattaacttt tgtacagata
     3181 ctttgcatgc tgaaaattat gtgatatcat taatcagaaa aaactgatat gtaaaatcat
     3241 atataaatca tattacacaa acaaaggagg ataatccaac atgagagaag tagtaatagt
     3301 aagcgcagcc aggacaccta taggaagttt cggaggcaca ttaaaaaatg ttgcggccag
     3361 aactctggga accatagcgg taaaaggagc catagataga gcaggcataa aacctgaaga
     3421 tgtggacgag gtaataatgg gatgtgttct tcagggagga ctcggacaga atatagcaag
     3481 acagatatcg attgatgcgg gcatacccaa agaggttcct tctatgacaa ttaacaaggt
     3541 ttgcggatcc ggcttaagag ctgttgaact ggcagcacag atcataaaag ccaatgatgc
     3601 tgagatcatt gtggcaggag gagcagaaag catgtccatg gcaccttatg taacaaggaa
     3661 agcccgttgg ggagccagga tgggtgatga caaactgctt gatatgatgg tatatgatgg
     3721 acttacagat gttttcggac agtatcatat gggaattact gcagaaaaca tatgtgaaca
     3781 gtgggggttg acgagggaag aacttgatga gttcgcagca gcttctcagc aaaaaactat
     3841 ggcagccata aaggccggca gattcaagga tgaaataatt cctgtagaga ttccacagag
     3901 aaagggagat cctatagttt ttgatacaga tgaatttcca aaggacggag ttacaaaaga
     3961 gagtataggt aaacttagac cggcattcaa aaaagatggt attgttaccg ccgccaacgc
     4021 gtccggtata aacgattccg gagcggcagt ggtggtaatg tcaaaagata aggcagatga
     4081 actgggactt gaatcaatgt gcacagtttt aagttatgct tctgcaggtg tggatccgag
     4141 tataatggga attggtcctg tgccggctac aaaaaaagca ttagaaaaag caggacttac
     4201 tgtggacgat ctggatctta tagaagccaa tgaagctttt gcggcccagt ctgtggctgt
     4261 aggcaaagaa ctgggattcg acaaagaaaa actcaatgta aacggaggag ccatagctct
     4321 tggacatccg ataggatcat caggctgcag gatacttatc acactgattt atgaaatgca
     4381 gaaaagagat tcaaaaaaag gtcttgccac attgtgtata ggcggcggac agggaacggc
     4441 aatgatagtt gaaagataaa acagcacaca agatcaaata tgataattat aaaacaggcg
     4501 gggtatatac cccgcctgtt ttataattat ggcgcaaaat aaaaacggat ccgaagatcc
     4561 gtttttgaat atttttttga agtatatatt tattcttctt ccggcggggt ggctataaca
     4621 aagggttgtt cagcaggttc ccctgtcagg cttgaacttt catgccacat gtagaagaat
     4681 ctttctgcgc catatgtata gtatccgtcc tgataattat cggaagtgtc ataggggtca
     4741 gccaggatga cgacatcatc tccgaaacta tccggtgtgc ccatgtcatc atagcctatg
     4801 ataacctgcc agtggccgct ccagtcgatc cagtcaacca ttatgggtgt ttctgctttc
     4861 aggtttttct ttacccattt tatgaaatcc tgatcggctg tatcactggt atcccagctg
     4921 aaagtcgcac tgccgtcttc ggggcttctt tccacatccc agcctatctc ttcaaagaac
     4981 ttggcaatgc cgtcgggacc tacgcctgtt tcatcactgg tatcagacat ctctgcaatg
     5041 ctcagttcat catatttttt ctcgtcatac atatcgtaat ggttaagcac cataagagct
     5101 gagcaggggc cgcaggaata ctctgtagtt tgctgatatg tttcaaattc cgggatcaca
     5161 gtaagagaat ccgttgattc cagattgtaa acatccaaag gcacaaaata gcctccgtca
     5221 aaatggtctc cacctccgtc gtaggatgaa gcccctgttg ttgtagtgtc atatccttcc
     5281 ggatacggga tagcatgctt ctcaaaagcg gcggtctcat catcgccgcc gcatgcggca
     5341 aaagcaaaaa ccattaccag tgtcaataag acaatcaata attttttggt tttcatgttc
     5401 atgtctcctc ctaaaatttc ataatacata attattcatc attacgatta tacacccgta
     5461 agaaggcaat agtaaagaat aaatcagaaa attttgaaat cggccttaag atccgattcc
     5521 atttcagccc ggtggggaag ggaatttatc ccggggttgc cgcctgtgtg gatgaatata
     5581 actgtttgat ttttatcgat caaacctgaa tctattatct taaggatgcc gttgaatgtt
     5641 ttgcccgtgt agcagggatc aaggataatg gcttcgcggc gagccatata ataaatagct
     5701 tctcttactt cggaaacggg gttattgtat gcgccgtatg tccagtcatg gtttatatca
     5761 aaatcctttt cggaggcgga aatctttatc cggttaccgt aaaaaccttt tattctttca
     5821 aaatatcctc tggcccaaac tgcaggttca gagga
//
LOCUS       DAONHK010000066         5815 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_10602_length_5815_cov_5.105208, whole genome shotgun sequence.
ACCESSION   DAONHK010000066 DAONHK010000000
VERSION     DAONHK010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5815
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10602_length_5815_cov_5.105208"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            79..822
                     /locus_tag="PLM92_03545"
     CDS             79..822
                     /locus_tag="PLM92_03545"
                     /inference="COORDINATES: protein motif:HMM:NF038196.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HPR24248.1"
                     /translation="MIFYFSGTGNSKWIAQEIAEKTGDRAESIVDMNRNGLVPYKAEK
                     GENLGFVFPVYSWGPPLIFMEFASKVTFDESNYLFGACTCGGSAGNAMEILSKIVPLC
                     SGFSFIMPNNYIIGSDVDTAEVAARKVKEAREKLPSVTEAIKEKKKVMDIKKGTLAGL
                     KSSLVCNLFNKYATNTDDFFVTEKCVSCGNCVSVCPLGSISLVDGKPKWEGNCTKCLA
                     CINRCPEEAIQSGKSTVSRGRYYFEKGTY"
     gene            complement(996..1391)
                     /locus_tag="PLM92_03550"
                     /pseudo
     CDS             complement(996..1391)
                     /locus_tag="PLM92_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005835399.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H"
     gene            1507..2307
                     /locus_tag="PLM92_03555"
     CDS             1507..2307
                     /locus_tag="PLM92_03555"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPR24249.1"
                     /translation="MSFSFCSIASGSSGNCYVVKSEKTTLLVDVGISGKKIFEGLEKT
                     GSSPSSLNGILISHEHSDHVKSLKTVCKKSKDAYIYSNINTWEHIKDMVPYERQITFE
                     KGKEFKAGDIMIRPFSISHDASDPVGFSFFYEGKQLSIVTDTGCITEEIFDEILEADM
                     IVLEANHDEEILKFCRYPYHVKRRILGDTGHLSNVAAAECISRLVKKNGKKRKFLLSH
                     LSRENNTPEVACLTVRNVLQEDGVYESENLAIEVIKRDTMSPLYELIK"
     gene            2327..2776
                     /gene="rlmH"
                     /locus_tag="PLM92_03560"
     CDS             2327..2776
                     /gene="rlmH"
                     /locus_tag="PLM92_03560"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HPR24250.1"
                     /translation="MNIKIICVGKSKERYWTEASAEYLKRLGAYCTCCLEEVKESISD
                     DPQEEGESILKKIRKSDYVISLEIRGKELSSEEMADMIEKTGIMGISDICFIIGGSLG
                     LSKKVTERADLRMSFSKMTFPHQMMRVILLEQIYRSFKIIRKERYHK"
     gene            2773..3951
                     /locus_tag="PLM92_03565"
     CDS             2773..3951
                     /locus_tag="PLM92_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006715174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HPR24251.1"
                     /translation="MNVIEKIKEYSLPLILGVVVAMVFANLWPELYQKIVYTSIAGEH
                     INFNWLVNDVFMVLFFASAGIEIVNDLSPGGALNPIKKAVTPLMATAGGVIGPIVLFF
                     VLNHFFGSPEFSNGWGITTATDIALAWLIARLIFGNRHPAVSFLLLLAVADDGIGLVI
                     IAVFYPDPRMPVEPGWLILIVVAMLIAFIMNRRDVKSFWWYFLICGTISWFGMHNAHL
                     HPALALVFIVPFMPHEGEAIPCNDPHGAFDAPEGTTLRSFEHFLVNIVEFGLFFFGFT
                     NAGVGFSEISSLTFIIMISLIVGKTVGISLFTLIADKVLRFGLPEGMRSRDVFTAGIV
                     GGMGLTVALFITESAFTDLTVQGAAKMGALFTVGAALIAFIVSKILRIQKSSEDDYFF
                     "
     gene            complement(3997..4314)
                     /locus_tag="PLM92_03570"
     CDS             complement(3997..4314)
                     /locus_tag="PLM92_03570"
                     /inference="COORDINATES: protein motif:HMM:NF024248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HPR24252.1"
                     /translation="MRIRKETDEMPESEVMLMAKISDALAHPARIKIFRYIMNCNKKI
                     IPVCNKGIVDEFDYAQATISQHMKTLVKSGLVDVRKEEKFSYYFVNIGMLMKYLDATK
                     RFE"
     gene            4444..5100
                     /locus_tag="PLM92_03575"
     CDS             4444..5100
                     /locus_tag="PLM92_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPR24253.1"
                     /translation="MKKAKQDINGIKWAMAGLIMIAAVNAMLAGFHVYKMIYLVEFAA
                     MLLSLAAWIMIFLASRSLSEVDTEFRNIFFISAICVIVTVFQGLLAAKYYFIDGLGNQ
                     AFIDLWVLLMYFITMLGLIFTYRFLLSGCNRLLINVSGGRKTMNWHKVFFRGAILILV
                     CMILMPFALLFPSIVKIILSVMVILLFLAVQGYMCRFIERAYNYIKGKGVTTGNEKRS
                     "
     gene            5084..5710
                     /locus_tag="PLM92_03580"
     CDS             5084..5710
                     /locus_tag="PLM92_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPR24254.1"
                     /translation="MKSDLKGLQIFEVGLIISIVTNFLGVLDLGKVFSFIISVISVCV
                     FMIMLTGAERLTEYDMGFIKTRNLLIAGVLLSSVIALLMGMAMADIEYWMTVTAVLFV
                     ISRAAVYIAVVGMAISGCVGISQRKKVRKSTKRLELENEYKEKCNRTWKVYLWLSVIS
                     IAIDVTGVMLINQDQFIWIALIFVFGTAVMVSGVLVAKRVNEAFDLCG"
BASE COUNT         1757 a         1058 c         1298 g         1702 t
ORIGIN      
        1 cattgtatca caaagggttg gtgcctcatt tttcattctt aactttacag accggaggaa
       61 cagtaaagga gaagaaaaat gatattctat ttttcgggaa cggggaattc caaatggatc
      121 gcacaggaga tagccgaaaa gaccggtgac agagccgaaa gcatagttga tatgaacaga
      181 aacggccttg tgccctacaa agctgaaaaa ggagaaaacc ttggatttgt ttttcctgta
      241 tattcatggg gaccgcccct gatatttatg gaatttgctt caaaggtgac ttttgatgaa
      301 agcaattacc ttttcggagc atgtacatgt ggcggaagcg ctggaaatgc catggagata
      361 ttatcaaaga tagtgcccct ctgttcggga ttttcattca ttatgccaaa caactacatt
      421 attggctccg acgtggacac ggccgaagtg gctgcacgaa aggtcaaaga agcaagagaa
      481 aaacttccct cggtcactga ggccataaaa gaaaagaaaa aggtcatgga cataaaaaaa
      541 ggcaccctgg cgggtctcaa gtcatccctg gtatgtaatc ttttcaacaa atacgccacc
      601 aacacagatg atttcttcgt cacagaaaag tgtgtttcat gcggcaattg tgtaagcgta
      661 tgcccccttg gaagcatatc ccttgttgac gggaaaccaa aatgggaagg taactgtaca
      721 aaatgtcttg cctgcataaa cagatgcccc gaggaagcca tacaaagtgg caaatcaacc
      781 gtcagcaggg gacgttacta ttttgaaaag gggacttatt aaaaaggggc ttcagcagat
      841 gggtccgctt tcaacattga ttttatgccg tggtttgcaa gaatatctgc acggcggttc
      901 atttcactta tatatttaaa atcatcgaat gaaaaccctg cgccgttcca ctccaggaat
      961 ttttcgtaga gtttgttcag catttcggca tcgcccttaa gatctgcatg cccttttacc
     1021 cgatagaaac ttatactgtg catatcaatg aaaggaagga gagcttccca gagatccctg
     1081 ttttcaacag gttttttttg tgaattcatc cagccgtttt tcatccagtt caaataccat
     1141 tttttcctga aacaatccat gagatatgag ctgtctgaaa aaatctcgat tatctgacct
     1201 tcttttttta tggcattaaa agccttaaga agagcagtca tctccatacg gttattagtt
     1261 gtatcgattt cgccgccgaa aagttctttt tgatgttctt cgtattcaag aacagcgccc
     1321 catccgcctg tgtttttttc gttctggttg ccggagcagg ctccgtcagt atatatctta
     1381 agtaccgcca tcagacacct cccgatattg atattatgca atatataaag tattatggca
     1441 agcagataca ttttgacaaa acaatggctt ggtgtataat gtatacatgc gtttttggag
     1501 aagaatatga gttttagttt ttgttctatt gcaagcggca gcagcggcaa ttgctatgtt
     1561 gtcaaaagtg aaaaaaccac tttgctggtg gatgtgggca taagcggtaa aaagatattc
     1621 gaggggctgg aaaagacagg cagcagccct tcctctttga acggtattct tatttcccac
     1681 gaacattccg atcacgtaaa aagtcttaaa acagtatgca aaaaaagcaa agatgcatat
     1741 atatattcca atataaacac atgggaacac ataaaagata tggtgcctta tgaaaggcag
     1801 ataacttttg aaaaaggcaa agaattcaaa gccggcgata taatgataag gccattcagc
     1861 atttctcacg atgcctctga tcccgtgggg ttttcatttt tttatgaagg aaaacagtta
     1921 agtatagtta ctgatacagg gtgcatcacc gaagaaattt ttgatgaaat acttgaagcg
     1981 gatatgatag tgcttgaggc aaatcacgat gaagaaatac taaagttctg cagatatccg
     2041 tatcatgtaa aaagaaggat cctgggagat acaggtcatc tttccaatgt tgccgctgca
     2101 gaatgtataa gcagacttgt aaaaaagaac ggaaaaaaaa gaaaatttct tctgtcgcat
     2161 ctgagtcggg agaacaatac tcccgaagtt gcatgcctta cggtaagaaa tgtacttcag
     2221 gaagatggag tatatgaaag cgaaaatctt gccatagaag taataaaaag ggataccatg
     2281 agccctttat atgaactgat aaagtagacc ggcggtacaa aacggaatga atattaaaat
     2341 aatctgtgta ggaaaatcaa aagagagata ctggaccgaa gcctcggcag aatacctgaa
     2401 aaggcttggg gcatactgta catgctgttt ggaggaggtc aaagaaagta tttccgatga
     2461 tccccaagaa gagggagaaa gcatactgaa aaaaataaga aaaagcgatt atgtgataag
     2521 cctggagata agaggaaagg agctgagctc cgaagaaatg gcagatatga tagaaaagac
     2581 agggattatg ggcataagtg atatatgttt tataatagga ggttccttag gactgtcaaa
     2641 aaaggtaaca gaaagagcgg atcttcgtat gtcattttct aaaatgacat ttcctcacca
     2701 gatgatgaga gtaatacttc tggaacagat atacagaagt tttaaaataa taagaaaaga
     2761 gagatatcac aaatgaatgt tattgaaaag ataaaagaat attctctgcc gcttatcctt
     2821 ggagtagttg tggcaatggt gttcgcaaat ctatggccgg aactttatca gaaaatagtg
     2881 tatacttcca tagcaggaga acacataaat ttcaactggc ttgtaaatga tgttttcatg
     2941 gttttgtttt ttgccagtgc gggcatagag atcgtaaacg acctttctcc cggaggtgct
     3001 ttgaatccca taaaaaaagc tgtgacccca ctgatggcca cagccggggg cgttatagga
     3061 ccgattgttt tatttttcgt actgaaccat tttttcggaa gccctgagtt ttccaacggc
     3121 tggggaataa ctaccgccac ggacatagct cttgcctggc tgatcgcaag actgattttc
     3181 ggaaacagac atcctgcagt aagttttctg cttttgctgg cggtggccga tgacggcata
     3241 ggactggtaa taatagcagt attctatccg gatccgagaa tgcccgtgga acccggctgg
     3301 ctgatactca ttgtagttgc aatgttgatc gcttttataa tgaacagaag ggatgtcaag
     3361 tctttttggt ggtattttct tatatgcgga acaataagct ggttcggaat gcataatgca
     3421 caccttcatc cggcactggc tcttgttttt atcgtacctt tcatgcccca tgaaggagag
     3481 gccataccct gcaacgatcc ccacggagct tttgatgctc cggaaggcac cacgcttaga
     3541 agttttgagc attttcttgt taatatagtt gaattcggcc ttttcttttt cggatttaca
     3601 aatgcaggag tgggattctc ggaaatatcc agtcttacat tcataataat gatttctctg
     3661 atagtgggaa agactgtagg catatcactg ttcaccctta ttgcggacaa ggttctcaga
     3721 ttcgggttgc ccgaggggat gcgcagcaga gatgtgttta ctgcaggcat cgtgggcggc
     3781 atgggcctta cagtggctct ttttataacg gaatccgcct tcactgatct gacagtgcag
     3841 ggagctgcca aaatgggagc tttgtttact gtcggcgccg cattgatagc ctttatagtt
     3901 tcaaaaatac tcagaataca aaaatccagt gaggacgact acttttttta aaaatcaaaa
     3961 tattatttta aagaaaaaag atttgaatag agttttctat tcaaatcttt ttgttgcatc
     4021 cagatatttc atcagcattc ctatgttaac aaaataatat gaaaattttt cttcctttcg
     4081 gacgtccaca agtcctgact tcacaagagt tttcatatgc tgtgaaatcg ttgcctgggc
     4141 ataatcaaat tcatcaacga tgcctttgtt gcaaacaggt attatttttt tgttgcagtt
     4201 catgatatag cgaaagatct ttattctggc ggggtgggcc agtgcatcgg atatctttgc
     4261 cataagcatc acttcggatt cgggcatttc atcggtttct tttctgatcc tcatatatag
     4321 tgtcctccgt gtaaaaaagt atcgcctaat aacgatactt catatcattg tttattgtca
     4381 agtcaatata tgattgaaaa acaaaataaa atgtgtgata atataatgca tcggaggcga
     4441 aaaatgaaaa aagcaaaaca ggatataaac ggaataaaat gggcaatggc aggactgatc
     4501 atgatcgctg cggtcaatgc catgctggca ggtttccatg tatacaaaat gatttacctt
     4561 gttgagttcg cagctatgct tctttctctg gctgcctgga taatgatttt cctggcatca
     4621 agatcattaa gcgaagtgga taccgaattt cgaaacatat ttttcatatc ggccatatgc
     4681 gttattgtaa ctgtttttca gggactcctg gcagcaaagt actattttat cgacggtctt
     4741 ggaaatcagg catttataga tctttgggta ctgctcatgt attttataac gatgctgggt
     4801 cttatattca catatcgttt tttgctatcc ggctgcaacc ggctgcttat taatgtgagc
     4861 ggcggcagga aaaccatgaa ctggcataaa gtattctttc ggggagctat ccttatactt
     4921 gtctgcatga ttctgatgcc ctttgctctg ctttttccaa gtatagtgaa aataatactt
     4981 tctgtcatgg ttatactgct gtttctggct gtacagggtt atatgtgcag atttattgaa
     5041 agagcttaca attatataaa aggaaaaggg gtaaccacag ggaatgaaaa gcgatcttaa
     5101 aggactgcag atatttgaag tcggactgat aatttccata gtcacgaatt ttctcggagt
     5161 gcttgatctg gggaaagttt tttcttttat aatttccgtt atttcggtat gtgtatttat
     5221 gataatgctg acaggagccg aaagacttac cgaatatgat atgggtttta tcaagacaag
     5281 gaatcttttg attgcaggtg ttttactgag ctcggtcata gcacttctca tgggtatggc
     5341 catggcagat atagagtact ggatgacggt aacggcagta ctgtttgtta tttcaagagc
     5401 ggcagtatat atagcagttg tgggcatggc gatttcggga tgtgtaggca tctcacaaag
     5461 aaaaaaagtc agaaaaagca caaaacgtct tgaactcgaa aatgaataca aagagaagtg
     5521 caacagaaca tggaaggttt acctatggct ttcggtgatc agcattgcta ttgatgtgac
     5581 aggtgttatg cttataaatc aggatcagtt catatggatc gctttgatat tcgtcttcgg
     5641 aacggcagtg atggtatcag gggtacttgt ggcaaaaaga gtgaatgaag cttttgatct
     5701 ctgcggctaa cataaacagg accttcttat aactatccgt ttcaggagct cgtttatcca
     5761 acagacgcaa atatatttgc gcctgttttt gatttgtgtt aaaatagctt tatat
//
LOCUS       DAONHK010000067         5722 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_10852_length_5722_cov_4.206282, whole genome shotgun sequence.
ACCESSION   DAONHK010000067 DAONHK010000000
VERSION     DAONHK010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5722
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10852_length_5722_cov_4.206282"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /locus_tag="PLM92_03585"
     CDS             <1..334
                     /locus_tag="PLM92_03585"
                     /inference="COORDINATES: protein motif:HMM:NF013869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MscL family protein"
                     /protein_id="HPR24255.1"
                     /translation="TAIITSLVNDIISPLLGLSVSVNFSSLQAETGGAVFTYGNFIMA
                     VINFLLVALVLFMIIRSINRMSNLKRKEEKEEVTRKACPYCKNDIPLEAVRCPYCTSE
                     LTSEEQIR"
     gene            544..1131
                     /locus_tag="PLM92_03590"
     CDS             544..1131
                     /locus_tag="PLM92_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPR24256.1"
                     /translation="MAFIILGACAFVLYFLYDINSVTMRKRFLFPAFGIGCMLLAIST
                     AGLVKISWDQERILSAGGIFLAVLGMVFGGLLIYTLFFALPFKKTYIKKEKDRAKPRI
                     CSKGMYALCRHPAVLWLIGLFIVLGAEFPTAEMMVGGTLFCVLDILYVIFQDLWTFPR
                     EFENYDEYRRKVPFLIPNGKSIKKCTDTLRGGNEI"
     gene            1121..2710
                     /locus_tag="PLM92_03595"
     CDS             1121..2710
                     /locus_tag="PLM92_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014357697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HPR24257.1"
                     /translation="MRFEDKLKNRSSMAIWQEYCGFLDLGIDDYMDIQNSLMKEQLQI
                     WSDCPLGREILKGKKINSIEEFRKDFPITEYSDYADILLQKRSDMLPAEPTIWIQTTW
                     EGGKHPVKLAPYTQGMLNTYKNNILACMLLGTSHGKGKFDVKANDTFLYGLAPLPYAT
                     GLFPRALADEIYIKFLPPVSEAEKMSFGERNKKGFKMGLSKGIDYFFGLGSVAYFVSM
                     SLSKMSGGGKSGAKTAYSPKFAFRYIKAKRACKKEGRELKPKDIFKLKGIMVAGTDNN
                     CYKDELEDLWGIRPMELFAGTEPSCIGTETWTRDGMYFFPDTCFYEFIPETEMMKSRE
                     FKNYHPKTCLMNEVIPGEKYELVISVLKGGAFMRYRVGDVYRCVGLENSEDQTKIPRF
                     HYIDRSPEIIDIAGFTRISQKAIENVVKISGLEVADWVAMKEYTENNKPKMHMYVEMK
                     PECLATSAVSSEILKEHLTVYFKYIDQDYKDLKRILGMDPLEVTVLKCGTFESYREKS
                     GKRLRKMNPSVYDLREVLACG"
     gene            2703..5276
                     /locus_tag="PLM92_03600"
     CDS             2703..5276
                     /locus_tag="PLM92_03600"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPR24258.1"
                     /translation="MDSNFVFVPIAALFCYVFLLMAFLAAKKTKVIYSFIWVLISGVL
                     WTGGSFCMRILMWPSYGLWYHVSILGLLVICYSFWYFLAEFTGSNPGIFRVIWLGLML
                     VVFALNATTGFFLAKPQVIELASGGIKFVYDLTWAVSLLFVACGAIVIHMFVLMIRFY
                     REDEVRKRQMRPLMTGVVMIFAGQLLFMLPFFEGIPVDILSAFVMVLCMMYAMHKRRL
                     FKLTLLVSKGSCYVASAILAALVFSNLIKPMEKFLTENFPRLASNDYLVIALIFALFT
                     IGFYYVMKKFIDRVFIKDEIIRSDIMKEFSGNVSKSLEINEILDSLVKVIRNALMVRN
                     VYVFIEQSDGKCYRMSKSLSPLDDKSVTIMTDNPIIKYMKSGSECLLMDEFRCLAVYK
                     SMWEKEKACFLSLNIECLMPLKDGDHLVGLVAISGKEKDTAFTYDDISFLSSVSSVGS
                     IAVKNSRLYERAYTEARTDELTGLLNRKYFYDTLQKEYSGIGKRSLALIIFNIDDFKL
                     YNQLYGFKEGDRALQKIAEIIRASVGDSGHIARYSGKEFAVILPGYDMLAAKNLAENI
                     RMQILNMNKSNKDYTFKVLTVSGGICVIPYAAGSVKELVDNADMAVYQVKRSGKNGIL
                     LSTGGAVERPAETGEETEHKEDIYSEYASTIYALTAAIDTKDHYTFNHSKNVAYYSSE
                     LAYACGLNQDSVEIIREAALLHDIGKIGIPEQILNKAEKLTREEYETVQGHVENSVGI
                     IRHLPSLDYVIPAVIGHHERYDGKGYPRRIGGEDIPLSARILAISDSFDAMISERSYK
                     EPYTVGYALEEIEHQAGKQFDPKLATLFVELVKTGKIKPDNEALSGKGYFGVEEEKAV
                     N"
     gene            complement(5281..>5722)
                     /locus_tag="PLM92_03605"
     CDS             complement(5281..>5722)
                     /locus_tag="PLM92_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPR24259.1"
                     /translation="TCPNCGCPLTPLHDQERGTEDHYPKTEAGKTDEKELETAKTRMK
                     YEADEKKRANRGLIAIVIILIAVVCAGAALYIINTMNEQAETEKKLEQLQNEEQEQND
                     DQVKEDNADDQEDAEPAQDPESSPEDSIVTDDGTVIEPPPEEPE"
BASE COUNT         1715 a         1065 c         1334 g         1608 t
ORIGIN      
        1 tacggctata ataacatcac tggtgaatga tatcatatca ccgcttctgg ggctctctgt
       61 aagtgtcaat ttctccagtc tgcaggcgga gaccggaggc gccgtattca catacggcaa
      121 tttcataatg gccgttataa acttcctgct tgtggcactt gttctgttta tgataataag
      181 gagcattaac aggatgtcaa atctgaagag gaaagaagag aaagaagaag ttacaagaaa
      241 agcatgtccg tactgcaaaa atgatatacc tcttgaggct gtaagatgtc cctactgtac
      301 ctctgaactc acatcggaag aacaaatacg ctgataaata tcagggatta tttataggta
      361 tcccaaaaag atacaataaa aaaaagcttg cagccacaaa atgttgtgtc tgcatttttt
      421 tagaccacaa ctttatagaa ataaagtcaa aaattcaaaa tatttacttg atatatttgg
      481 aaattgcgtt tatcattata gagaaaaacg tttatttaca aatgctttga taaggtgggg
      541 gcaatggcat ttattatact tggcgcctgt gcatttgtgc tttatttctt atatgatata
      601 aacagtgtga ctatgcggaa gagatttctt tttcctgcat tcggtatcgg ctgtatgctt
      661 cttgctattt ccacggccgg gcttgtgaaa atctcgtggg atcaggaaag gattttgtct
      721 gccgggggaa tatttctggc agtactgggc atggtttttg ggggactgct tatatataca
      781 ctttttttcg ctttgccttt caaaaagaca tatataaaga aggaaaaaga cagggcgaaa
      841 ccgaggatct gttcaaaagg gatgtacgca ctttgccgtc atccggcagt gctttggctg
      901 atagggcttt ttatagtatt gggtgcagag ttcccgaccg cagagatgat ggtgggagga
      961 acgctttttt gtgtgcttga tatactgtat gtgatttttc aggacctttg gactttcccc
     1021 agggaatttg aaaattatga tgaatacaga agaaaggtgc catttctgat acctaatgga
     1081 aaaagcataa aaaaatgtac tgatacttta agaggcggca atgagatttg aagataaact
     1141 gaaaaaccgt tcgtcaatgg ccatatggca ggaatactgt ggatttctgg atcttggaat
     1201 agacgattat atggacatac aaaacagcct catgaaagag cagctgcaga tttggagtga
     1261 ttgtcccctt ggcagggaga tacttaaagg caaaaaaatc aattcaatag aagagttcag
     1321 aaaggatttt cccataaccg agtattcgga ttatgctgat atacttttgc agaagagaag
     1381 tgatatgctt ccggcggagc ccaccatatg gatacagacc acatgggaag gcggaaaaca
     1441 tcccgtgaaa cttgctccct acacccaggg aatgctgaac acatacaaaa acaatatact
     1501 tgcatgtatg cttctgggta ccagtcacgg taaaggcaag tttgatgtta aagcaaatga
     1561 cacattcctt tacggtctgg cacctcttcc ctacgcaacg ggactttttc caagagcgct
     1621 ggcggatgag atatacataa aatttctccc tccggtttcg gaggccgaaa agatgtcctt
     1681 tggagaaaga aataaaaagg gattcaaaat ggggctttca aaggggatag attacttttt
     1741 cggacttgga agcgtggcct atttcgtaag catgagttta tccaaaatga gtggcggagg
     1801 caaaagcgga gcaaaaacag cctactcccc aaaatttgca ttcagatata taaaagccaa
     1861 aagagcatgc aaaaaagaag ggagagagct taagcccaag gatattttca agttgaaggg
     1921 cataatggtg gcagggaccg ataataactg ttataaggat gagcttgaag atctttgggg
     1981 aataagaccc atggagcttt tcgcaggaac agaaccctca tgcataggaa ctgaaacctg
     2041 gaccagagac ggaatgtatt ttttccctga tacatgtttc tatgaattca ttcccgagac
     2101 ggaaatgatg aaaagcagag agttcaaaaa ctatcaccca aagacttgcc ttatgaacga
     2161 ggtaataccg ggagaaaaat atgaactggt catatccgtt ctgaaaggcg gagcattcat
     2221 gcggtacagg gtcggagatg tttacagatg tgtaggactc gaaaacagtg aagatcagac
     2281 aaaaataccc aggttccatt atatagacag gagcccggag ataatagata ttgcgggttt
     2341 tacgagaata tctcaaaaag ccatcgaaaa tgtcgtaaaa atatcagggc ttgaggttgc
     2401 cgactgggtg gcaatgaagg agtacaccga aaataacaag cccaagatgc acatgtatgt
     2461 ggagatgaag cccgagtgtc tggcaaccag tgctgtgagt tcggaaatac tgaaagagca
     2521 tcttacggta tatttcaaat atatagatca agactataaa gaccttaaaa ggatccttgg
     2581 aatggatccc cttgaagtca cggtactcaa gtgtgggact tttgaaagtt acagagaaaa
     2641 aagcggcaaa agacttagaa agatgaatcc gtctgtttat gatctgaggg aggttttggc
     2701 ctgtggatag caactttgtt tttgtcccca tagcggcact tttctgttat gtattcctgc
     2761 tgatggcatt tttggcagcg aaaaagacaa aagtgatcta ttcattcata tgggtactca
     2821 ttagcggtgt tctttggacc ggcggttcct tttgtatgcg tatacttatg tggccgtcct
     2881 atggcttgtg gtaccatgtt tcaatccttg gtcttttagt gatatgttac tctttctggt
     2941 atttcctcgc ggagttcacc ggttcaaatc ccggtatatt cagagtgata tggctggggc
     3001 ttatgctggt tgtatttgca ttaaatgcaa caacgggatt tttcctggca aaacctcagg
     3061 taattgagtt ggcatccggc ggcatcaaat tcgtttatga tctcacatgg gcagtcagtt
     3121 tgcttttcgt agcctgcggg gccatcgtta tccatatgtt cgtgctgatg ataagattct
     3181 accgggagga cgaagtaagg aagcgccaga tgagacctct tatgaccggg gtcgtaatga
     3241 tattcgccgg gcagcttctg tttatgctcc cgttctttga agggatccct gttgatatac
     3301 tttcggcttt cgtgatggtc ctctgcatga tgtatgcgat gcataagagg aggcttttca
     3361 aacttactct tctggtttca aagggaagct gttatgtggc atcagccatt cttgctgcac
     3421 tggttttttc gaatcttata aaacccatgg aaaaattcct cacagaaaat tttccgagac
     3481 tggcatccaa tgactatctg gttatagccc ttatatttgc gttgttcact ataggatttt
     3541 actatgtcat gaaaaaattc atagacagag tatttataaa agatgaaata ataagatcag
     3601 atataatgaa ggaattcagc ggtaatgtct ccaagagcct ggaaataaat gaaatactgg
     3661 acagtcttgt caaggtaata agaaacgctc ttatggtaag gaatgtttat gtattcattg
     3721 aacagagtga cgggaaatgt tacaggatgt ccaagagctt aagccctctt gacgataaat
     3781 ccgtaacaat aatgaccgac aaccccatta ttaaatatat gaaaagcggc agtgagtgtc
     3841 ttctgatgga tgaattcaga tgccttgctg tatataaatc catgtgggaa aaagaaaaag
     3901 catgcttttt atctcttaat atcgaatgcc ttatgcccct taaagacgga gaccatttgg
     3961 tgggtctggt ggccatatcc ggaaaagaaa aggatacggc tttcacttac gatgatataa
     4021 gtttcctttc atcggtgagc tccgtgggtt ccatcgcagt gaaaaactca aggctttatg
     4081 aaagagcata tacagaagca agaactgatg aacttacagg actgctaaac agaaagtatt
     4141 tttatgatac tcttcaaaag gaatatagcg gaataggtaa gagatccctt gctcttatta
     4201 tattcaacat agatgatttc aagctttaca accagctcta tggttttaaa gaaggagaca
     4261 gggccctgca gaaaatagca gagataataa gagccagcgt gggtgacagt ggccatatag
     4321 ccaggtacag cggtaaagaa ttcgctgtta tactgccggg atatgatatg ctggctgcga
     4381 aaaacctggc agaaaacata aggatgcaga tactcaacat gaataaaagt aacaaggatt
     4441 atacattcaa agttcttact gtaagcggcg gtatctgtgt cataccctac gctgccggaa
     4501 gcgtgaagga actggtggac aatgcggaca tggcagttta tcaggtcaag cgttccggaa
     4561 agaacggtat acttctttct acgggtggag ctgtcgaaag acctgccgag acaggagagg
     4621 aaacggaaca taaagaggac atatattcgg aatatgcatc cacgatatat gctctgactg
     4681 ctgctataga taccaaggac cattacacat tcaaccattc caagaatgtt gcctattatt
     4741 cttccgagct ggcctatgcc tgcgggctga atcaggattc tgttgaaata ataagagaag
     4801 cagctcttct tcatgacata ggtaaaatag gcataccgga acagatcctt aataaggccg
     4861 aaaaacttac aagagaagaa tatgagacag tccaaggaca tgtagaaaac tccgtgggga
     4921 taattcgtca tcttccctca ttggattatg taatacctgc ggtaataggt catcatgaaa
     4981 gatatgacgg aaaaggatat cccagacgca taggagggga ggacattcct ctttcggcca
     5041 ggatactggc aatatccgat tcttttgatg cgatgatatc agaaagatct tataaggagc
     5101 catacactgt gggttatgcc ctcgaagaaa tagaacatca ggcaggaaaa cagtttgatc
     5161 ccaaacttgc cacccttttt gtggaactgg tcaaaaccgg caaaataaaa ccggacaacg
     5221 aagcattgtc cggaaaaggt tattttgggg ttgaagaaga aaaagctgta aactgacttt
     5281 ttattcgggc tcttcaggag gcggttctat aacggtaccg tcatcggtga ctatgctgtc
     5341 ttccggagaa ctttcgggat cctgtgcagg ttcggcatct tcctgatcat cagcattatc
     5401 ttctttgacc tgatcgtcat tttgttcctg ttcttcattc tgaagctgtt ccagcttttt
     5461 ttctgtttct gcctgctcat tcatagtgtt tattatatat aaagccgcac cggcacagac
     5521 tacagctata agaattatta ctatggcaat aagaccccgg ttggctcttt ttttttcgtc
     5581 agcctcgtat ttcattctgg tttttgctgt ttccagttct ttttcatcgg ttttgcctgc
     5641 ctcggttttg ggataatgat cttccgttcc tctttcctga tcgtgcaggg gtgttagagg
     5701 gcatccgcag tttgggcatg tt
//
LOCUS       DAONHK010000068         5713 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_10874_length_5713_cov_4.547367, whole genome shotgun sequence.
ACCESSION   DAONHK010000068 DAONHK010000000
VERSION     DAONHK010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5713
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10874_length_5713_cov_4.547367"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            82..777
                     /locus_tag="PLM92_03610"
     CDS             82..777
                     /locus_tag="PLM92_03610"
                     /inference="COORDINATES: protein motif:HMM:NF021506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HPR24260.1"
                     /translation="MYSGLLLLVAIIFPLPAIIKGIKKGQNPYRTVTDGIITAGVGFV
                     FIYILSSVSAGGIAVETEESIRLITESLGEKTQEYKEYLARAVMVLPAAMLCLSSVCG
                     YVEYKGLYRALKKDNIKAVEMPAFRDFSWPRNSIYGWFLIFIISWTMKAAGSGVGEIT
                     AANINVIFETAFAFQGAALVFMIFFLKKIPKALAVILVITAWLVPFGKNVLFIAGIAD
                     IVLGIRARISRKQ"
     gene            794..2557
                     /locus_tag="PLM92_03615"
     CDS             794..2557
                     /locus_tag="PLM92_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005999151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HPR24261.1"
                     /translation="MNEKKSGRIFSDYMPLPMCKLDTRGKVLNASPGIDQVFLYDGIK
                     GADIFALTGIKHEQFTDAAKNKETLTIYRNEKTFRIVPAFVGKKEPVGIALYFMDVTD
                     FEQAEKRYEEEKLCVIVVNVDNFDELLSGTGDDRQASLASEIDKTIRQWGSSMKASVT
                     KYRDNMYIIMLQNKYLEEQIEKKFPILDQVREIETEVDFPVTLSIGCGKDCENPTEAE
                     DFAVEARDLALGRGGDQAIVKEGDKFYYYGGKTQTVEKSNKGKSRIIGHALTRLIESA
                     ENVLIMGHKNPDMDAFGAALGIYRLARPINKETYIIINKYNEALAPIYKAVKETGDYR
                     IITSRKALNIAKKETLVIIVDTHRTSITDCPEILEIAEKSAVIDHHRKAEDFIKNPTL
                     VYIEPYASSTSELVTEILQYSRDKKSIVKIEAEALLAGISVDTNRFAVKTGVRTFEAA
                     AWLRKSGADTTTVKRLFQTNLETFKTRANCMANADFLEDGIAISICEGRNVNAQIINS
                     QVADELLTIKGIKASFVAGCNEDGRTVLSARSLGDINVQTIMEKFDGGGHLTTAGAQM
                     DISPRDAIEKVKEILKKEKTE"
     gene            2554..2997
                     /gene="rplI"
                     /locus_tag="PLM92_03620"
     CDS             2554..2997
                     /gene="rplI"
                     /locus_tag="PLM92_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HPR24262.1"
                     /translation="MIVILLKDIKGTGKAGEVVKVSDGYARNMLLPKGLAKEATEGNV
                     RSLEKQKAIVAEKEAESREKAQKLSEQLGSIALNIKTKGGEGGRLFGSVTSKDISEAL
                     KEQYGIDLDKKKIILESPIKETGEKRVSVKVYPEISAVLKVIISV"
     gene            3016..4335
                     /gene="dnaB"
                     /locus_tag="PLM92_03625"
     CDS             3016..4335
                     /gene="dnaB"
                     /locus_tag="PLM92_03625"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005582428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HPR24263.1"
                     /translation="MERIPPHNEDAEKSVLGAAMLDKDALYDVMEEVRPQDFYNQNHR
                     EIFEAITELSRRNSPVDVLTVAEELKKRKSLEMAGGRGYIATLSAGVPTTANAREYAK
                     IVAEKAALRNLIKTAGDIMEKGYEDKVAAEDIIDYAEKGIFEVAQNRQKKEMMPIREI
                     LMKNLDEIDERSKMEGNITGVISGLIDLDDKTAGFQKSDLIIIAARPSMGKTAFALSV
                     AQQAAVKGKAKVLFFSLEMSKEQLGQRLLSMEAKVDSQKIRAGTLAREEWDRIAMALD
                     ILGEGDISIDATPGLKLMEIKNKCRRLKAEKGLDMVILDYLQLMEHEGRAESRQQEIT
                     SLSRGLKQLAREIECPVLVLSQLSRAPEQRTEHRPILSDLRESGAIEQDADLVLFLYR
                     DEVYFPEETEKPGICEVNIAKNRNGPTGTVEITWQSQYTRFANKAGR"
     gene            4338..4541
                     /locus_tag="PLM92_03630"
     CDS             4338..4541
                     /locus_tag="PLM92_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006427234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HPR24264.1"
                     /translation="MNRITEEMKVYEILDKYPKLEKILLQHGLFCSGCPGSNTETIHE
                     AAEGHGIEPHILLKKLNEALEQD"
     gene            4541..>5713
                     /locus_tag="PLM92_03635"
     CDS             4541..>5713
                     /locus_tag="PLM92_03635"
                     /inference="COORDINATES: protein motif:HMM:TIGR01446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HPR24265.1"
                     /translation="MTFVKKHAKTACLTDTSVENIFINDYMVSAPGDYVKIYLLTLMY
                     TDLDRQASCDDIAKTLRLSPEEVDKAWDYWEKQGVVRKKGDQREFVVLKEQLYGQKID
                     EKKASGQKVAGAILENEELKSMYKTIEKTIGRLLSGTELTEIIEWIEENGATPEVIAQ
                     AYAYCAANKKDNVRYVGTVVKNWASQGLTSVLDVEKYLNENDRRHYVYKRVMQALGFA
                     RNATEEERRIIDSWTEKMGYGINDILEACKKTSGIPNPNINYINKVLINRNDGKNQSM
                     ATGAATVKRFYEHLREKAAAAAHEKTKEVYEKVPAIREIDEEIKECSIELSKVMISDR
                     QNKKQQIKKLREEGESLTKARAVMLTENGFPIDYMEIHYLCGLCKDTGTKDSGEQCV"
BASE COUNT         2068 a         1014 c         1396 g         1235 t
ORIGIN      
        1 acacaacatg tagtagagga caaaagaagc actttatgtt ataatatttt ggcatgaaat
       61 gaaaaagaaa gaggacattt aatgtattca ggcttattgc ttcttgtggc aataatattt
      121 cctttaccgg ccataataaa aggcataaaa aaaggacaaa acccatacag aacagtaaca
      181 gacggcatta ttactgccgg agtggggttt gttttcattt acatattatc ttcggtttcg
      241 gcaggcggga tagccgttga aacagaagaa agcataagac ttattacaga aagtctcgga
      301 gaaaaaacgc aagaatacaa agagtatctt gccagggccg ttatggtact gcctgcagcg
      361 atgctttgtc tttcttcagt atgcggatat gttgaataca aaggacttta cagggcactc
      421 aaaaaagata atataaaagc cgtagaaatg ccggctttca gagatttctc ctggcctcgc
      481 aacagtattt acggatggtt tctcatcttt attatatcct ggaccatgaa agcagcaggc
      541 tccggagttg gagaaatcac agcagccaat ataaacgtga tatttgaaac ggcatttgca
      601 tttcagggtg cagccctggt tttcatgata ttttttctaa agaaaatacc caaagcactg
      661 gcggtcattc tggttataac ggcatggctc gtaccctttg ggaaaaatgt tttgttcata
      721 gcgggtatag cggatatagt tctgggaata agagcaagga tatcccggaa gcagtagaca
      781 gaggtgttat ataatgaatg aaaaaaaatc gggcagaatt ttttcggatt acatgcccct
      841 tcccatgtgt aaactggata caagaggcaa ggtactaaat gccagtccgg gaattgatca
      901 ggtttttctt tatgacggga taaagggtgc ggacatattt gccctcacgg gtatcaagca
      961 tgaacagttt acagatgccg caaaaaacaa agagacgctt actatatacc ggaatgaaaa
     1021 aacattcagg atagttcccg cattcgttgg aaaaaaagaa ccggtgggta tcgctcttta
     1081 ttttatggat gtcacggatt ttgaacaggc ggaaaaaaga tatgaagaag agaagctgtg
     1141 tgtgatagtt gtcaatgtgg ataattttga cgagcttctt tcgggcacgg gagacgacag
     1201 acaggcatct ctcgcttcgg aaatagacaa aaccataaga cagtggggct catctatgaa
     1261 ggcttcagtg actaaataca gagacaacat gtatataatc atgcttcaaa acaaatacct
     1321 ggaagaacag atagaaaaaa agtttcccat actggaccag gtaagggaaa tagaaacaga
     1381 agtggatttt ccggtcacct taagcatagg atgcggcaaa gattgtgaaa accccacaga
     1441 agcagaggat tttgcagttg aagcaagaga tctggctttg ggaaggggag gagatcaggc
     1501 aatagtaaaa gagggggaca aattttatta ttacggagga aagacccaga ccgtagaaaa
     1561 aagcaacaaa ggaaaatcca gaataatagg acacgctttg acgaggctga tagaaagcgc
     1621 agaaaatgtt cttataatgg gacacaaaaa tcccgatatg gatgcattcg gtgccgcttt
     1681 gggcatatat cgcctggcga gacccataaa caaagaaaca tatataatca taaataaata
     1741 taatgaagct ctggcgccga tatacaaggc ggtgaaggaa accggtgact acaggataat
     1801 caccagcaga aaagctctta atatcgcaaa aaaagaaacc cttgtgataa ttgtggatac
     1861 ccacaggacg agcataacag actgtccgga gatacttgag atcgcagaaa aatccgctgt
     1921 tatagatcat catcggaaag cagaggattt cataaagaac ccgacacttg tatacatcga
     1981 gccctatgca tcttccacct ccgagcttgt aacagaaatt cttcaatatt caagagacaa
     2041 aaaaagcata gtgaaaatag aggcggaagc tttgctggca gggatatcgg tagacaccaa
     2101 cagatttgcc gtcaaaacag gagtcagaac ctttgaagct gccgcatggc taaggaaatc
     2161 aggagcagat actacgacag tcaaaagact ttttcagacc aacctggaaa cattcaaaac
     2221 aagagcaaac tgtatggcca atgcggattt tctcgaagat ggcatagcaa tatccatatg
     2281 cgaaggcaga aatgtgaatg cccagataat aaattcccag gtggcagatg aacttctgac
     2341 cataaaagga ataaaagcca gttttgttgc gggatgcaat gaagacggac gtaccgtttt
     2401 aagcgcaaga tctttagggg acataaatgt gcagacaata atggagaaat ttgacggagg
     2461 aggccatctt acaacggcag gtgcacagat ggacataagt ccccgggacg ccatagaaaa
     2521 ggtaaaagaa atactgaaaa aggagaaaac cgaatgatag taatattatt gaaagatata
     2581 aaaggaacag gaaaagccgg agaagtggta aaagtaagtg atggttatgc caggaacatg
     2641 cttctgccaa agggactggc aaaggaggcc acagaaggaa atgtaaggag cctggaaaag
     2701 cagaaagcca tagttgcgga aaaggaagcc gaaagcagag aaaaagccca aaaactttct
     2761 gagcaactgg gtagcattgc cttaaatatc aagacaaagg gtggagaagg cgggcgactc
     2821 tttggctcgg tgacatctaa agatatcagt gaagccctga aagagcagta cggcatagac
     2881 cttgacaaga aaaaaatcat acttgagtcg cccataaaag aaacgggcga aaaaagggtg
     2941 agcgtaaaag tttatcccga aatctcagca gtactaaaag ttattatatc cgtataagag
     3001 caaggagact atcaaatgga aaggatccct ccccataatg aagatgcgga aaaatccgtt
     3061 ttgggtgcag ccatgctgga caaagatgca ctgtatgatg tcatggaaga agtaaggcct
     3121 caggactttt ataaccagaa tcacagagaa atattcgaag cgataacaga actttccaga
     3181 aggaattcac cggtggatgt tcttactgtg gcagaggaac taaaaaaaag aaaatctctt
     3241 gagatggcag gaggaagagg gtatatagca actctttctg ccggtgtccc caccacggca
     3301 aatgcaagag aatatgcaaa gatagtggca gaaaaagctg ctttaagaaa cctcataaaa
     3361 acagcaggag atataatgga aaaaggctat gaggacaaag tggcagctga agatataata
     3421 gattatgcag aaaagggtat atttgaagtc gctcaaaaca ggcaaaaaaa agaaatgatg
     3481 cccataaggg aaatactcat gaaaaacctg gatgagatag atgaaaggtc aaagatggaa
     3541 ggcaatatca ccggagtcat aagcgggctt atagatctgg atgacaaaac ggcaggattt
     3601 cagaagtctg accttattat catagctgca aggccctcca tgggtaaaac agccttcgcc
     3661 ttaagtgttg ctcagcaggc agcggtcaaa ggcaaagcca aagtgctttt cttcagtctg
     3721 gagatgtcca aggaacagct gggacagaga ctgctttcca tggaagcaaa ggtggattcc
     3781 caaaaaataa gagcgggtac cctggcaaga gaagagtggg acaggatagc aatggctctt
     3841 gacatcctgg gagaagggga tataagcata gacgcaactc ccggactcaa actgatggag
     3901 ataaaaaaca aatgcagaag gctgaaggca gaaaaggggc ttgacatggt catacttgac
     3961 tatcttcagc ttatggagca tgaaggaagg gcagaaagcc gtcagcagga gataacatct
     4021 ttatcaagag gattaaagca actggcaaga gaaatagaat gtccggtgct ggtactttca
     4081 caactttcaa gggcgcctga gcagagaact gaacacagac cgatcctttc cgatttaaga
     4141 gaatcgggag ccatagaaca ggatgcggat ctggtgcttt ttctttacag agacgaagta
     4201 tatttccccg aggaaacaga aaaaccgggc atatgtgaag tgaatatagc aaaaaacaga
     4261 aacggtccca ccggaacagt ggagatcacc tggcaaagcc agtataccag attcgcaaat
     4321 aaagccggga ggtagtgatg aacaggataa cagaagaaat gaaagtatat gaaatacttg
     4381 ataagtatcc caagctggaa aaaatacttt tgcagcatgg acttttttgt tccggatgtc
     4441 ccgggtcaaa tacggaaacc attcatgaag ccgctgaagg gcatgggata gaaccgcata
     4501 tacttttgaa gaaattaaat gaggcactgg agcaggactg atgacatttg taaaaaaaca
     4561 cgcaaagacc gcttgtctta cagataccag cgtagaaaac attttcataa atgattatat
     4621 ggtctccgct cccggagact atgttaagat atatcttctt acacttatgt acacggatct
     4681 tgaccgacag gcaagctgtg acgatattgc caaaaccctg cggctttctc ctgaagaggt
     4741 ggataaggca tgggactact gggaaaagca gggagtcgta agaaaaaaag gagatcaaag
     4801 ggaatttgtt gttttaaaag agcagcttta cggtcagaaa attgacgaaa aaaaggcttc
     4861 agggcaaaaa gtcgcaggag cgatcctcga aaacgaagag ctcaaaagta tgtacaaaac
     4921 catagaaaaa accataggaa gacttttgag cggaacagaa ctaacagaga taatagaatg
     4981 gatagaagag aacggggcaa ctcccgaagt gattgctcag gcctatgctt actgtgctgc
     5041 gaataaaaag gacaatgtca gatatgtggg gacagtggta aaaaactggg cttctcaggg
     5101 actcacaagc gttctggatg tagaaaagta tctgaatgaa aacgaccggc gccactatgt
     5161 ttacaaaaga gttatgcagg cactgggatt tgcaaggaat gccaccgaag aagagcgtcg
     5221 tataatagac agctggacag aaaaaatggg ttatggtata aatgatatac ttgaggcatg
     5281 taaaaaaacc agcggtatac caaatccaaa tatcaattat ataaataaag tgttgataaa
     5341 ccgtaatgac ggaaaaaatc aaagcatggc gacaggtgcg gccacggtaa aaagatttta
     5401 tgaacatctg cgtgaaaaag ctgcggctgc ggctcatgag aaaacaaaag aggtctacga
     5461 aaaagttcct gccataaggg agatagatga agaaataaaa gaatgcagta tagaattatc
     5521 aaaggtaatg atctcagatc gtcaaaacaa aaagcagcaa ataaaaaaac tcagagaaga
     5581 aggagaaagc ttgaccaaag ccagagctgt tatgctcacc gaaaacggtt ttcccattga
     5641 ttatatggaa atccattatc tttgcggatt atgcaaggat accggaacaa aagattcggg
     5701 agaacagtgt gta
//
LOCUS       DAONHK010000069         5600 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_11190_length_5600_cov_3.614247, whole genome shotgun sequence.
ACCESSION   DAONHK010000069 DAONHK010000000
VERSION     DAONHK010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5600
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11190_length_5600_cov_3.614247"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..948)
                     /locus_tag="PLM92_03640"
     CDS             complement(<1..948)
                     /locus_tag="PLM92_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017812977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HPR24266.1"
                     /translation="MSKIIIISNRLPVTVKRKGKSLEYIESIGGLTTGLKSYHEKADS
                     IWAGWPGISDEDISSRQKKNIEKKLKENYDCIPVFLSQEEIDQYYYGFCNKTIWPLFH
                     YFSSEAEYDPEKWEAYKEVNQKFFEKIKPSISRNDTIWVHDYQLMLLPSLIKQAYPST
                     KVGFFLHIPFPSFEIFRLLVWREEILQGLLGSDLIGFHTYDYVRHFLSSTRRLLGLED
                     KLNVISYEDKYVRVDSFPMGIDYERFTGEYNKKGLAPEVLQINEIKETKTILSIDRLD
                     YSKGIPERIKAFARFLKEYPEYKEKVRLNLIVAPSRVEIE"
     gene            complement(1148..2236)
                     /gene="recA"
                     /locus_tag="PLM92_03645"
     CDS             complement(1148..2236)
                     /gene="recA"
                     /locus_tag="PLM92_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006000622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HPR24267.1"
                     /translation="MNKDDKEKALEAALGQIEKQFGRGSIMRLGDDSIKMNMEAISTG
                     SISIDIATGIGGIPKGRIVEVFGPESSGKTTLTLHMIAEAQKQGGKAAFIDAEHALDP
                     SYAKNLGVNVDELLVSQPDTGEQALEICEMLVRSGALDIVVIDSVAALVPKNEIQGDM
                     GDSHVGLQARLMSQALRKLAGAINKSNASVVFINQLREKVGIMFGNPEVTTGGRALKF
                     YSTMRFDVRRIESIKSGDSVKGNRTRVKIVKNKVAPPFKMAEFDIMYGEGISKEGDIL
                     DCAVEAKIIEKAGSWFSFEGDRIGQGRENAKEYLRENKATADKVEGMLLDVIKKSKEP
                     DEGHSEGPAKGKAADAPPKVDEDGVIIE"
     gene            complement(2406..4577)
                     /locus_tag="PLM92_03650"
     CDS             complement(2406..4577)
                     /locus_tag="PLM92_03650"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HPR24268.1"
                     /translation="MTENKEEKISFFSRLKTRYIPQKRKGKVFMILSIALIVLGYIYF
                     LLNKEYYTNIYDDGTPYFALKKFVFTSLIFVVLLILVFFRNRFNDKTNFIINLVMLEA
                     VPFAAFVAIEYLNDTKFLNVNFIFVLLNLMIILSVMGVGFIITGRMKMGYVFGVIVVA
                     VFGIMNYFIYMFRGIPLLASDFRSLTTAANVAGNYEFYLNYRAFIFLVACFGICLVVS
                     KLKEVRLKGMFPRLKVTGVLAVSVCLILVFSVFTNTFGIEIKTFRPMKGYHNNGAVLT
                     LVGSIRKLIVEKPENYSAETAKTIAEKYKKEEQAQDTQKDDGEADAKTEVIASEKTPN
                     MIIVMDEAFADVGAVGTGLATNKEVMPFFKSLEENTIRGESYVSFFGGGTANSEFEFL
                     TGNSMAYLPEGATPYQLFLNDYMPSLNNTLEKQGYQGNMAFHSFEANGYSRPTAYEYL
                     KFEDFISIEDVAEELSYVRGLATDKSHFNKIISLYEKYDKKSEAPFYMFNVTMQNHSP
                     FNISDPAMDMDIEVTTDLLPAEEGSNKEVDRYLNLINMSDEALGELITYFRGVDEPTV
                     VVFFGDHQPRLPEALYKRLLGGNDTQLTAESLMQKYSAPFVIWANYDIPEEKDVKISM
                     NYLSSYMLDSLDMEMTDYNKFLMRLHDRVPVVTAFGFWGSDGVFYENQQDILAGSNPN
                     PETVQQVMTSPFDADLEEYKIMQYNNMFDVKNRVKDFFYLK"
     gene            complement(4570..>5600)
                     /locus_tag="PLM92_03655"
     CDS             complement(4570..>5600)
                     /locus_tag="PLM92_03655"
                     /inference="COORDINATES: protein motif:HMM:NF016357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047355 - CDP-glycerol
                     glycerophosphotransferase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HPR24269.1"
                     /translation="SFKKPSYHRYMEEEYRNTEVIKTYGRTYEQTLAKAKYWITNYRV
                     LDHIYPGKDQVYVQCWHGTPLKRLGYDLDHSDNAMNSTDEIWDKYKIDAEKFTWMISP
                     SSFATEKFISAWNLKEFGREDVIIEEGYPRNDILTNYDEELKDFTKECIGISDMGEKK
                     IILYAPTWRDNQHVAGLGYTYDMHLDLDRLQRELGEDYILLCRLHYLVANSIDFEKYE
                     GFIYDVSKYDDINELYIISDLLITDYSSVFFDYANLKKPILFYMYDLEEYRDDIRGFY
                     IDINELPGEIITEEDRLIQAVKEVGDGFVPDEKYMAFNSRYNYLDDGNAAKRTVRKIL
                     GKDNNSND"
BASE COUNT         1361 a         1326 c         1115 g         1798 t
ORIGIN      
        1 ctcgatctcg actctggagg gggctactat aagattcagt ctgacttttt ctttgtattc
       61 gggatactcc ttaaggaatc ttgcaaaagc ttttatcctc tcaggtatgc ctttgctgta
      121 atccagacgg tctatggaaa ggatcgtctt agtttctttt atctcgttga tctggagcac
      181 ttcaggtgcc aggccttttt tgttgtattc tcctgtaaat ctttcgtaat cgatacccat
      241 ggggaacgag tccacccgga catatttgtc ttcgtaactt atgacattga gtttgtcttc
      301 aaggcccagg agtctccttg tgctgctcaa aaaatgtctc acataatcat aagtgtggaa
      361 tcctatcagg tctgatccca gaagtccctg gagtatctct tctctccata caagaagcct
      421 gaatatctca aatgagggga aaggtatgtg aagaaaaaat cccaccttcg tggaaggata
      481 tgcctgtttt atcagggaag gaagaagcat aagctgataa tcatggaccc atatggtgtc
      541 attcctgctg atggagggtt tgatcttttc gaaaaacttc tgattgactt ctttgtaggc
      601 ctcccatttc tcgggatcat attccgcctc cgatgaaaaa taatgaaaaa ggggccatat
      661 tgtcttgttg cagaaaccgt agtaatactg atctatctct tcctgagaaa gaaagaccgg
      721 tatgcagtca taattttctt tcagcttttt ttctatgttc tttttttgtc ttgatgaaat
      781 gtcctcgtca gaaatgccgg gccagcctgc ccagatgctg tcagcctttt cgtggtagct
      841 ttttagtccg gtggttagcc cccctatact ttcgatatac tcgagacttt tcccttttct
      901 ttttaccgtg accggtaaac gattggatat tattattatt ttgctcatat tgatcacctc
      961 tggaatatta ttctgttaaa tcaacaaaat aaagttctct tgattatgct gtattatacc
     1021 atacatccaa tactaattcc aaatacgtat gatcaaaaaa aatgaaaaac tgcccgggtc
     1081 ccggcagata gagacaatgc tgctgcaata aaaaagagcc ggtcatctgt tccggccggc
     1141 tcctctgcta ttctattatt acgccgtcct catccacttt cggtggggca tcggccgcct
     1201 taccttttgc cggtccttct gaatgacctt catcgggctc ctttgatttt ttgatcacat
     1261 ccagaagcat gccttctacc ttgtccgccg tagctttgtt ttctctgaga tattctttgg
     1321 cattctccct gccctggcct atcctgtctc cttcaaaact gaaccaggaa cctgcctttt
     1381 ctattatttt ggcttccacg gcgcagtcca ggatgtcgcc ttccttggag atgccctcgc
     1441 cgtacattat atcgaactca gccatcttga agggcggtgc aactttgttc ttgactattt
     1501 tgactcttgt tctgttgccc ttgacactgt cgccggattt gatgctctct atccttctta
     1561 catcaaagcg catggtggaa tagaacttaa gcgcccggcc cccggtggtc acctcggggt
     1621 tgccgaacat tatgcctact ttttctctca gctggtttat gaacacgacg gaagcattgg
     1681 atttgtttat ggctcctgcc agttttcgca gggcctggga catgagtctg gcctgcagcc
     1741 ccacatgact gtctcccata tccccctgta tctcgttctt gggcaccagt gccgccacgg
     1801 aatctatcac aacgatatcc aaagctccgc tcctgaccag catctcgcat atctccagtg
     1861 cctgttcccc ggtgtccggc tgggagacca gcagttcatc cacattgacg cccaggttct
     1921 tggcatatga tggatccagc gcatgttccg catctatgaa tgcagccttg ccgccctgct
     1981 tctgagcctc ggctatcata tggagagtaa gtgtggtctt gcccgaggac tcgggtccga
     2041 agacctccac tatcctgccc ttgggtatgc ctcctatgcc ggtggctata tcaatactta
     2101 tggatcctgt ggatatggct tccatgttca tcttgatgga atcgtctccc aatctcatta
     2161 tggagcctct gccgaactgt ttctctatct gtcccaaagc cgcttccagg gctttttctt
     2221 tgtcatcttt atttatggcc acttgtttat acctccgttt tcataagtgc gaacatttgt
     2281 tcttttttta taatatccta tttttgccgg caggtcaagt atttttgccg atgtttccaa
     2341 ttatttttca ctgtaaatat ttggtatttt tattatattc ttacatcagc atcgtgtcaa
     2401 catatttatt tcaaataaaa gaaatcctta actctatttt ttacgtcaaa catattgttg
     2461 tactgcatta tcttatactc ttcaagatct gcatcaaagg gggatgtcat cacctgctgc
     2521 actgtttccg gatttggatt ggaaccggca agaatgtcct gctgattctc ataaaaaact
     2581 ccgtcggaac cccagaagcc gaaggccgtc accaccggca ccctgtcatg aagccgcata
     2641 agaaatttat tgtagtccgt catttccata tccagggagt ccagcatata cgaagaaagg
     2701 tagttcatgc ttatcttaac gtctttttct tcaggtatat catagttggc ccaaataaca
     2761 aatggcgcac tgtatttctg cataagactt tcggctgtga gctgagtatc gttccctccc
     2821 agcagtctct tataaagggc ttcgggaagt cttggctggt gatctccgaa gaaaaccacc
     2881 acagtgggtt cgtccacacc ccggaaatat gttatcagtt ctcctaaggc ctcatcgctc
     2941 atattgataa ggttcagata tctgtccacc tctttgttgc tgccttcttc cgccggcaga
     3001 aggtccgtcg tcacctctat gtccatgtcc atggcgggat cgcttatgtt gaagggactg
     3061 tggttctgca tggtcacatt gaacatataa aaaggtgcct ctgatttttt atcgtatttc
     3121 tcataaagac ttattatctt gttgaagtga gatttgtcgg tggcaagacc tcttacatat
     3181 gaaagttcct ccgccacatc ttcaatggag ataaaatctt cgaatttcag atattcgtaa
     3241 gccgtgggcc tgctgtaacc gttggcctcg aaactgtgaa aagccatgtt cccctgatat
     3301 ccctgtttct cgagggtgtt gttcagagaa ggcatataat cattaagaaa cagctgataa
     3361 ggagtcgctc cttccggcag atatgccatg ctgttccccg tcaagaattc gaattcgctg
     3421 ttggcggtac ctcctccgaa aaacgaaaca taggattccc ccctgatggt gttttcttca
     3481 aggcttttga aaaatggcat tacttctttg tttgtcgcaa gtccggtccc gacagctccc
     3541 acatcggcaa aggcttcatc cattactatg atcatattgg gagtcttttc gctggcaatg
     3601 acttcggtct ttgcatcagc ttccccgtca tccttttggg tatcctgtgc ctgttcttct
     3661 tttttgtatt tttcggctat ggtttttgcc gtctccgccg aatagttttc cggtttttca
     3721 actataagtt tccttatgct tcccacaagg gtaagaactg ctccgttgtt atgatatccc
     3781 ttcatgggcc ggaaggtttt tatttctata ccgaatgtat ttgtaaaaac agagaacaca
     3841 agtataagac atactgaaac cgccagaacg ccggtcactt tcagtctggg gaacattccc
     3901 ttcagtctta cttccttcag cttgctgaca accagacata tgccgaaaca tgccacaagg
     3961 aagatgaagg ctctgtaatt aaggtagaat tcatagttgc ccgcaacatt ggctgcggtg
     4021 gtaagagagc gaaagtccga tgccaaaagg ggtatcccgc ggaacatata aatgaaatag
     4081 ttcatgatcc cgaaaacagc cacaacgatg acaccgaaga catagcccat tttcatccgg
     4141 ccggttatta tgaagcccac acccattaca gacagtatga tcatcagatt aagaagaacg
     4201 aatataaaat taacatttaa gaatttggtg tcgttaagat attcgatggc cacaaatgcg
     4261 gcgaagggca cagcctccag catcacaagg tttattataa agttggtctt gtcattgaat
     4321 ctgtttctga aaaacaccag tatcaaaagt acaacgaata tcaaagaagt gaaaacgaat
     4381 ttttttaatg cgaaataagg tgtcccgtca tcataaatat ttgtatagta ctccttattc
     4441 agaagaaaat atatataacc cagtacgatc aaggcaatgg aaagtatcat gaacactttg
     4501 ccttttcttt tttgcgggat ataccttgtc ttcagcctgc taaaaaatga gattttttct
     4561 tccttatttt cagtcattgc tattgttgtc ctttcctaat attttccgta ctgttctttt
     4621 ggctgcgttc ccgtcatcca ggtagttgta gcgagagttg aatgccatgt atttttcatc
     4681 gggcacaaac ccgtctccaa cttcctttac tgcctgtatc agcctgtctt cttctgttat
     4741 gatctctccg ggcagttcat ttatgtcgat ataaaaaccc ctgatatcgt ctctgtattc
     4801 ttcaagatca tacatataaa acaaaatggg ttttttgagg tttgcataat caaaaaatac
     4861 gctggaataa tccgtgatga gcagatcgga tataatataa agctcattta tatcatcata
     4921 ttttgaaaca tcatatatga atccttcgta cttctcaaaa tcaatactgt tggccaccag
     4981 ataatgaagt ctgcataaaa gtatatagtc ttcccccagt tccctttgga gtctgtccag
     5041 atcaagatgc atatcatagg tataccccag gccggccaca tgctggttgt ctctccaggt
     5101 cggggcataa agtattatct tcttttctcc catgtcagaa atgcctatac actcttttgt
     5161 gaaatccttg agttcttcat cgtagtttgt cagtatgtca ttcctgggat acccttcttc
     5221 tattatcaca tcttctctgc cgaactcctt aaggttccat gcacttatga atttttccgt
     5281 agcaaaagaa gacggcgata tcatccatgt gaatttctcc gcatctattt tgtatttgtc
     5341 ccatatctca tcggtggaat tcatggcatt atcggaatga tccagatcat atcccagtct
     5401 tttcagggga gtgccgtgcc agcactggac atatacctga tccttcccgg gatagatgtg
     5461 gtccagtacc ctgtagttgg ttatccagta cttggccttt gccagagtct gttcatatgt
     5521 cctgccatag gttttgatga cttcagtgtt tctgtattcc tcttccatgt atctgtggta
     5581 agatggtttc ttgaacgacc
//
LOCUS       DAONHK010000070         5507 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_11439_length_5507_cov_3.791453, whole genome shotgun sequence.
ACCESSION   DAONHK010000070 DAONHK010000000
VERSION     DAONHK010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5507
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11439_length_5507_cov_3.791453"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..624)
                     /locus_tag="PLM92_03660"
     CDS             complement(<1..624)
                     /locus_tag="PLM92_03660"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HPR24270.1"
                     /translation="MDKKLKQIIQESTNMVFFGGAGVSTESNIPDFRSEQGLYNAEHK
                     YGMCPERIISHSFFVDDTETFYDYYKNNMIYREARPNKAHMALALLEKQKKLKAVVTQ
                     NIDGLHQMAGSKNVFELHGSVLRNNCMDCGEGYDLDYIMDPKNCKGSIPKCRECGGTV
                     KPDVVLYEEGLSEEMINGAVRAIAEADTLIVGGTSLVVYPAAGLINYF"
     gene            731..1393
                     /locus_tag="PLM92_03665"
     CDS             731..1393
                     /locus_tag="PLM92_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HPR24271.1"
                     /translation="MNTKVLRNLSYGVYVVSSMDGERPTGCTANSIMQITSSPPTIAA
                     SINHDNYTNSCIQKTGMFSVSILGEDINPGVIGHFGFQSGKDVNKYDDTPHEMISGIP
                     VVKSAGSYLICKVVDSMETSTHTVFLGEIIDADFLENGTPMTYAYYHNVIKGKSPKNA
                     PTYIPEEEKKMSGEKAIWKCTVCGYEYDENKEEVAFADLPEDWKCPVCLQPKTKFEKV
                     EV"
     gene            complement(1402..2181)
                     /locus_tag="PLM92_03670"
     CDS             complement(1402..2181)
                     /locus_tag="PLM92_03670"
                     /inference="COORDINATES: protein motif:HMM:TIGR03915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03915 family putative DNA repair protein"
                     /protein_id="HPR24272.1"
                     /translation="MVDYLYDGTFEGLLTCIYHNYYGEKASGIFPAAGYQSHMLGGYK
                     ETETDEEKADRVYDAVARRISDYDLGRIYKVFMSSAPEKENKILNYLRLGFKKGSCIS
                     LLHGDPTVFAVQAAEKKVNNEIHRLKGLVRFSAMGSCEYPVYYSAVEPDNDVIEFLAR
                     HFCDRFKNQAFIIHDIKRSKALIASGGSWYISSFFSSELPPFSEDEQKFRDLWKKYFD
                     STAIKERTNARCQKNFMPVRYWKHLTEMQPRIDSNDQEKLP"
     gene            complement(2162..3565)
                     /locus_tag="PLM92_03675"
     CDS             complement(2162..3565)
                     /locus_tag="PLM92_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014297519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA modification/repair radical SAM
                     protein"
                     /protein_id="HPR24273.1"
                     /translation="MDNIYEKLKILADSAKFDVSCSTSGVGRNNNGRIGNTQIAGCCH
                     SWSADGRCISLLKVLFTNKCIYNCEYCLNKRTADTQRASFEPDELARLVIEFYKRNYI
                     EGLFLSSAVEVSPDHTSEKILKALRLLRNNYGFAGYIHAKIIPGTSSELVHAIGLEAD
                     RLSVNTELPSRQSLSLLAPQKKPEHIFAPMKQITNTLIENRALVGPGTMFKGIDINRE
                     LNYLSPADGRDIIPRPGQRRNNEFAPAGQTTQIIIGADDSSDRDIIKVSEKIYHTFKM
                     KRVYYSAYIPVVSSPFLPDALTAPPLSREHRLYQADWLLRFYGFDSEEILPKGDPYLD
                     PELDPKASWALRNLNLFPVEINKASKEELLRIPGVGNLSVLRIIRQRRISAVKYEDLK
                     RIGVVLKRARHFITCSGKYYGERNFLPENIKKEILQTSCGTQMSMFETQLIPSFKAGN
                     LPISERKEGHRNGGLPL"
     gene            3695..4933
                     /locus_tag="PLM92_03680"
     CDS             3695..4933
                     /locus_tag="PLM92_03680"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HPR24274.1"
                     /translation="MKKLKTALVLSGGGARGSYQIGVWKALKKIRIKPDIVVGTSVGA
                     INGAMIVLGKFSDAADFWLSAETDEIFAVSSKNDISFKTPVKIRVTSQFFGMPMEDAF
                     GYAKDILINGGADSSRLFDLLKKHIDETKLRRSLTDYGLVTVSLTDLKSHYLFTDDIP
                     VGELHDFILASASCFPAAKYHEINITKYIDGGYADNMPVEMALEKGAERIIAVDLNAV
                     GKISKTSLKKAEENAEEFILISSTADLGNFLVFDPKTAARNMKLGFLDAMKAFGRYEG
                     KYLTFRKKSFGEVKVKEADTAGRIFGLDPSILYSRKSFEKSLKKKLFPAPDLPDDIKK
                     ISDIKKALAALLDHAGPAGLTVIIAEDIMRKGDESYFLKTPVSKLFKDQIIAANYIAG
                     SGMLVPSGPVFEHFPGGSFV"
     gene            complement(4871..>5507)
                     /locus_tag="PLM92_03685"
     CDS             complement(4871..>5507)
                     /locus_tag="PLM92_03685"
                     /inference="COORDINATES: protein motif:HMM:NF018816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HPR24275.1"
                     /translation="ISFPLFLCYNVKYYRYIINNEGQTVNMAQTNTSKIILTALMMGI
                     IIVSTIMIRVPIPFTQGYVHLGDGMIFLGVLILGWKYGAVAGGVGSMLGDIIGGFAMW
                     APWTLVIKGSMALIMGLIIAFAAKKERSHIQMMAMQLAGMIFAGLFMVMGYFVAEGLM
                     YGNWVVAALGVPWNIGQFAVGMIIAAAVKAALYKTPAGKMFEYRTGRDKHS"
BASE COUNT         1555 a         1219 c         1103 g         1630 t
ORIGIN      
        1 aaaatagttt ataagtcccg ccgcaggata taccacaagg gaagtgcctc caactataag
       61 agtatctgct tccgctatgg cccttacagc accgtttatc atttcttccg aaagaccctc
      121 ttcataaagg accacgtccg gtttgactgt tcctccacat tctctgcatt taggtatact
      181 ccccttgcag tttttgggat ccattatata gtcaagatca taaccctctc cgcaatccat
      241 acaattgttt ctgaggactg atccatgcag ttcaaaaaca tttttactgc ctgccatctg
      301 atgcagccca tctatattct gagtcaccac tgctttgagt tttttctgtt tttccagtag
      361 ggcgagggcc atatgtgctt tgttgggtct ggcctccctg tatatcatgt tatttttgta
      421 gtaatcatag aaagtttcgg tatcatcaac aaaaaaactg tgggaaatga tcctttccgg
      481 gcacatacca tatttatgct cagcattata aagtccctgc tcggatcgga aatcaggtat
      541 attgctttcc gtcgaaactc ctgctccgcc aaagaagacc atattcgtgc tttcctgtat
      601 tatctgtttt agctttttat ccattgtttc agctcccttt ttatcgtgta tattataaca
      661 cattgattga gattttttta ttttaatgct ataatccttt tgtgaccatt tctatacgaa
      721 ggaggagttt atgaatacaa aagttcttag aaatttatca tacggagtct atgtggtttc
      781 atcaatggac ggggaaagac ctacaggctg cacagctaac agcataatgc agataacatc
      841 atcccctccc acaattgccg ccagtataaa tcatgataat tatactaaca gctgcataca
      901 gaaaacaggt atgttttccg tatccatatt aggcgaagat ataaatcccg gcgtgatcgg
      961 gcattttggt tttcagtccg gcaaagatgt caacaaatat gatgatactc cccatgagat
     1021 gatatccggg atccctgttg tgaaatcagc gggaagttat ctgatttgca aagttgtgga
     1081 ttccatggaa acatcgaccc acactgtatt tcttggtgaa ataatagacg cagactttct
     1141 tgaaaacggc acacctatga catatgccta ttatcacaat gttataaagg gcaagagccc
     1201 caaaaacgca ccaacatata tacctgagga ggaaaagaaa atgagtggag aaaaagccat
     1261 atggaaatgt accgtttgcg gttatgagta tgatgaaaac aaagaagaag tagctttcgc
     1321 agatctgccg gaagactgga aatgtccggt ttgcctgcag ccaaaaacta aatttgaaaa
     1381 agtagaagtc taaaacatcc cttaaggcag cttctcctga tcattagaat cgatccgagg
     1441 ctgcatttca gtaagatgtt tccagtagcg caccggcatg aagtttttct gacaacgggc
     1501 attggtgcgc tcttttattg ctgtcgaatc aaaatatttt ttccaaagat ctctgaattt
     1561 ttgttcgtct tcggaaaaag gcggcagttc actgctgaaa aaagaagaaa tataccagct
     1621 tccgcctgat gcaataaggg ctttgcttct ttttatgtca tgtatgataa aagcctggtt
     1681 tttaaatctg tcgcaaaaat gacgggccag aaactcgatg acatcattat ccggttctac
     1741 agcgctataa tagaccggat attcacagct acccattgct gaaaatctta ccagtccttt
     1801 cagcctgtgg atctcattgt ttactttttt ctctgctgcc tgaaccgcaa aaactgtcgg
     1861 gtcgccgtgc agcaaactta tgcatgaccc ttttttgaat ccaagcctca aataattaag
     1921 tattttattt tctttttcgg gggctgaaga catgaagact ttatatatcc tgcccagatc
     1981 gtagtcggat atcctgcggg caacagcatc atataccctg tctgcttttt cttcatctgt
     2041 ctctgtttct ttatatccac ccaacatatg tgactgatat ccggcagcag gaaatatgcc
     2101 ggatgccttt tctccgtaat aattatgata tatgcatgtc aggagtccct cgaaagttcc
     2161 gtcataaagg taatccacca ttcctgtgtc cttcctttct ttcggatatc ggcaggttcc
     2221 ctgccttaaa cgatggaatc agctgtgttt caaacattga catctgtgta ccgcaactgg
     2281 tctgaagaat ttcttttttt atgttttcag gaagaaaatt cctctcgccg taatactttc
     2341 cggaacaggt gatgaaatgt ctggctcttt taaggacaac tcctattctt ttcaggtctt
     2401 catatttcac tgccgatatt cttctctgcc tgattatacg caaaaccgac agattaccca
     2461 caccgggaat gcgaagaagt tcttcttttg aggctttgtt tatttcaacc gggaaaaggt
     2521 tcaggtttcg cagtgcccag gaagccttag gatccagttc cggatccaga taaggatctc
     2581 ctttgggaag gatctcctcg gaatcaaatc cataaaacct caaaagccag tctgcctggt
     2641 aaagcctgtg ttccctggaa agaggaggcg cagtcagcgc atcaggaaga aaaggagatg
     2701 aaaccacagg tatatatgca ctgtaatata ctcttttcat tttgaaagta tgatatatct
     2761 tttcgctgac ctttattata tccctgtcag aagaatcatc tgctcctatt attatctgtg
     2821 tcgtctgccc ggcgggagcg aattcgttgt tccttctctg cccgggcctt ggaatgatgt
     2881 ctcttccgtc tgcaggactc aagtaattca gttctctgtt gatatctatt cccttaaaca
     2941 ttgtacccgg ccccaccaaa gcacggtttt ctatgagagt atttgttatt tgcttcatgg
     3001 gtgcgaatat atgctccggc tttttttgag gggcaaggag cgaaaggctt tggcgactgg
     3061 gcagttccgt attaacactt aatctgtctg cctcaaggcc tatcgcatga acaagctcag
     3121 aggatgttcc gggtattatt tttgcatgta tatatcctgc aaacccgtaa ttatttctta
     3181 gtaaacgtaa agctttcaat attttttcgg atgtatgatc cggggagact tcaacagccg
     3241 agctcaaaaa aagaccttct atataatttc gcttataaaa ttctatcaca agccgcgcca
     3301 gttcatcagg ctcaaaggat gccctctgtg tatcggcagt tcttttgttt aaacagtatt
     3361 cacaattgta aatacatttg ttagtaaaca gtaccttgag cagagaaata catctgccat
     3421 cagcagacca tgagtggcag cagccggcta tttgagtatt gcctattcgt ccgttattgt
     3481 ttctccctac gccgcttgtg gagcaggaaa catcaaactt ggcgctgtct gccagtattt
     3541 tcagtttttc atatatgtta tccatatcct tacaccctat cccgaacttg tgtttgatta
     3601 taacatataa cgaacatatg ttcaagccct gcatgtgtct cccctttgtt gacatttgga
     3661 agtctcaaga ttactatgaa atagagggga aaaaatgaaa aaactcaaaa cagctcttgt
     3721 tttaagcgga gggggcgcaa ggggcagcta tcaaatagga gtatggaaag cccttaaaaa
     3781 aataaggata aaacccgaca tagtcgtcgg aacatctgta ggcgccatca acggagctat
     3841 gatagttctg ggtaaatttt cagatgctgc tgatttctgg ctttcggcag agacagatga
     3901 gatttttgct gtttcatcaa aaaatgacat atcattcaaa actcccgtta agattagagt
     3961 aacctcccaa tttttcggta tgcccatgga agatgctttc ggatatgcaa aggatatcct
     4021 tattaacgga ggtgctgaca gcagccgcct ttttgatttg ctgaaaaagc atattgatga
     4081 aacaaaacta cgcagatccc tcactgatta tgggcttgtg accgtaagtc ttacagatct
     4141 gaaaagccac tatcttttta cagacgacat accggttgga gaactgcacg atttcattct
     4201 ggccagcgct tcatgtttcc ctgcagccaa gtatcacgag ataaatataa ccaaatatat
     4261 cgatggtgga tacgcagaca atatgcccgt tgaaatggct ttggaaaagg gtgccgaacg
     4321 catcatagca gtggatctta atgccgtagg caagatcagt aagacttcac tgaaaaaggc
     4381 agaggaaaat gcagaagaat ttattcttat aagtagcact gcagacctgg gaaatttcct
     4441 ggtttttgac ccgaagaccg cagcccgcaa catgaaactg ggatttctcg atgcaatgaa
     4501 agccttcgga cgatatgagg gcaaatatct taccttcagg aaaaaatcct ttggagaagt
     4561 aaaagtaaaa gaagcggata cagcaggtcg aatcttcggg cttgatcctt caattctata
     4621 cagccggaaa agttttgaga agtcccttaa aaagaagctg tttccggctc ctgaccttcc
     4681 tgatgacatc aagaaaattt cagacatcaa aaaagccctt gccgctcttt tggatcatgc
     4741 aggtccggca ggacttactg ttatcattgc agaagatata atgagaaagg gcgatgaaag
     4801 ttattttcta aagactcccg ttagcaaact ttttaaggat caaatcattg ccgcgaatta
     4861 tattgcgggt tcaggaatgc ttgtcccttc cggtcctgta ttcgaacatt ttcccggcgg
     4921 gagttttgta taaggccgct ttgaccgcag ccgctataat catcccaaca gcgaactgtc
     4981 ctatattcca tggcacaccc agtgcggcaa ccacccagtt tccgtacatg agaccttccg
     5041 caacaaaata gcccattacc atgaaaagtc cggcaaaaat cattcctgcc agctgcatag
     5101 ccatcatttg tatgtgactt ctttcttttt ttgctgcaaa tgcaataata agacccatta
     5161 taagtgccat tgatcctttt atcacaagcg tccagggagc ccacatggcg aacccgccaa
     5221 ttatatctcc cagcattgaa cctactcccc ctgcaacagc tccgtacttc cagccaagta
     5281 taagaacacc gagaaatatc atcccgtctc ccagatgcac atatccctgg gtaaatggta
     5341 tgggcacccg tatcataata gtagaaacta taatgatacc catcatcagt gccgtaagta
     5401 ttattttgct ggtgtttgtc tgtgccatat tgaccgtctg cccctcattg tttataatat
     5461 atctgtaata tttaacattg taacataaga aaagaggaaa ggaaatg
//
LOCUS       DAONHK010000071         5335 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_11916_length_5335_cov_4.685038, whole genome shotgun sequence.
ACCESSION   DAONHK010000071 DAONHK010000000
VERSION     DAONHK010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5335
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11916_length_5335_cov_4.685038"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..755
                     /locus_tag="PLM92_03690"
     CDS             <1..755
                     /locus_tag="PLM92_03690"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HPR24276.1"
                     /translation="VEIILTIFMFISGASYYLYFLALRSGIRTFLHDSEFKLYSLIFV
                     LFSVMIATGLLVQGEYDTFGEAIRYSSFQTVSILTTTGFATADYIFWPTFCQITLLLL
                     FFIGGCSSSTAGGIKVIRVFVLIKLIKRTIGMKLHPSAVVTVKVNDNKILPGDTVSGI
                     ASFVFLYIFLIFAGSILLSLENIDLLTCFTATASCIGNIGPAFGEVGPMLNYSFLSEG
                     SKMLLSFMMIAGRLELFTLIMLFSGKFWNPYH"
     gene            766..2235
                     /locus_tag="PLM92_03695"
     CDS             766..2235
                     /locus_tag="PLM92_03695"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HPR24277.1"
                     /translation="MYFHARTIARITGLIMTITGLAMIPPFLFALYYSDSSTALALGV
                     SCMASVIPGALMYFFLRGSKKGLQVRDGYLTVTICWAVCSLLGALPYWLSGQVPSLID
                     AFFESCSGYTTTGATVLIERNLTHGLLLWKAVTHWLGGMGILVFIIAVLPALGVGGHK
                     IASAEAPWASLNKIAPRTSDLAKLLYLIYISFTLMEFALLSLSKMSLFEAVINSLGSV
                     STAGLLLHPDGVAFYDSFYAEIVISVFTILSSVNYMMYIYIIKRNFRDLKDNVEIKVF
                     LGIIASVTLLITASLRISGTYDSLFTAFRHAFFQVTSISTTSGYTIGDFDVWPAFSKA
                     LLFTLFFIGGCAAATSGSIKVIRIIIMFKLIMRGFYKRLHPLAVRAVKIKGKNISAPM
                     VSSVTSFIILYFILYLFSVVVLSFQGLDMETTLSSPAGLMSNIGVGFGQVSEGDFSCY
                     SAPLKLYMSLLMVVGRLEIFTVFLLFVPSFWNPNRSNTR"
     gene            complement(2236..4245)
                     /gene="rnr"
                     /locus_tag="PLM92_03700"
     CDS             complement(2236..4245)
                     /gene="rnr"
                     /locus_tag="PLM92_03700"
                     /inference="COORDINATES: protein motif:HMM:TIGR02063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HPR24278.1"
                     /translation="MKKRKNMKRVTGTLQKHKRGFGFVITETASGIKEEKDIFISGKD
                     MNGAMNGDLVEVELIPARLWKNSPEGIVAGILSRSVSEVVGTFEKNGRFGFVIPDDRR
                     LNEDIFVKKKDFYCAERGDKVVAEIIKYPSRTDSAQGRIKQIISRSGQPGGDIRSLIR
                     QYGITEEFPSKAASEAKKLFAKGITEEDKEERRDLKDKMIFTIDGADSKDFDDAVSID
                     MLENGNYLLGVHIADVSSYVKEGSALDKEALKRGTSIYVIDQVVPMLPAELSNGICSL
                     NPLEDRLCISVDMEIDKAGEVIRHEIYESIINSKARMVYTDVSDMLEKNSRELIKKYD
                     SGPSGVYDSIVKMHELAKILEQRRDMRGSLDFDLEEAHITLNKEGIPVEVSTCERRSG
                     NKLIEEFMLKANEVIAEHFALLKIPFVYRIHEKPSPDNITELMLFLKGFGIYPEGGTK
                     DIKPKTLSRILKSISGGANENIISTVMLRSMQKAEYNTQCLGHFGLALKYYCHFTSPI
                     RRYPDLMIHRIIKSWLKGELMSSAERTAGPDAKVAAKKTRAYKRLTKEAAEISSAKER
                     QAIELEREAEKMKKAEYMSYHVGEEKSGIISGVTNYGIYVQLENTVEGMIKLSQLFDD
                     YYEYDPAGHRVCGRRKGKTYSLGDTIDIRVRSVSIEDREINFDMI"
     gene            complement(4428..>5335)
                     /locus_tag="PLM92_03705"
     CDS             complement(4428..>5335)
                     /locus_tag="PLM92_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009525513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HPR24279.1"
                     /translation="MHSTVWPIMFIAAGIYIAYEAAGLYGIALAAVGMLSTTGMTVAV
                     DAYGPVSDNAGGIAEMADLPEDVRGITDKLDSVGNTTAAIGKGFAIGSAALTALALFA
                     AYATVTDLKVISLLEPIVIVGLFIGGMLPFLFSAMTMNSVGKAAHQMIEEVRTQFRND
                     PGIMEGTSRPDYARCVDISTKAALKEMVAPGLLAIIAPLAVGLVLGTQALGGMLAGSL
                     TAGVLVAIMMANAGGAWDNAKKYIEEGHHGGKGSEAHKAGVVGDTVGDPFKDTSGPSI
                     NILIKLMTIVSVVFAPVFVAVGGLI"
BASE COUNT         1229 a         1302 c         1018 g         1786 t
ORIGIN      
        1 acgttgagat catactaacg atctttatgt ttatttcagg tgcaagttat tatctttact
       61 tcctggcttt gcgtagcggc atcaggactt ttttgcatga ctccgaattc aaactgtatt
      121 ctctgatttt tgtattattc tctgttatga tagcaacagg tcttcttgtt caaggtgaat
      181 acgatacatt cggagaggca attagatatt catcctttca aaccgtttct atacttacaa
      241 ctacaggctt tgctactgca gactacatat tctggccgac tttctgtcag ataactctgc
      301 ttttactttt cttcatcgga ggatgctctt cttcaaccgc aggaggcata aaagtaataa
      361 gagtatttgt gctgataaaa ctcataaaaa gaaccatcgg aatgaagctg catccaagcg
      421 cagttgtgac tgttaaagtg aatgataata aaatacttcc gggagatacg gtttccggca
      481 ttgccagttt tgtctttctt tatatttttc ttattttcgc agggagcata ctgttgtccc
      541 ttgaaaatat agatttgctg acatgtttta ctgctactgc atcttgcatt ggcaatatcg
      601 gtccggcctt cggcgaggtg ggaccaatgc tcaactacag ctttttatca gaaggctcca
      661 aaatgctttt atccttcatg atgattgccg gaagactgga actttttacg cttatcatgc
      721 ttttttccgg gaaattctgg aatccatatc actagaggta caaaaatgta ttttcatgca
      781 cgcaccatag ccagaataac aggattgata atgaccatta caggacttgc catgatcccg
      841 ccatttttgt ttgcacttta ttacagcgac tctagtacag cccttgcttt gggtgtatca
      901 tgcatggcat ctgtgattcc cggcgctttg atgtacttct ttcttagagg ctcaaaaaaa
      961 ggtttgcagg tccgcgacgg ttatcttact gttactattt gctgggccgt ctgttccctt
     1021 ttgggtgctt tgccttactg gctttccggg caggttcctt ctctcataga tgcattcttt
     1081 gagtcatgtt cgggctatac aaccaccgga gccaccgtcc ttatagaacg caaccttacc
     1141 cacggccttc ttctctggaa agctgttacc cactggcttg gaggaatggg tatacttgtt
     1201 tttataatag cagtacttcc tgccctcggt gtcggcggtc ataagatagc ctctgccgaa
     1261 gctccctggg cctcccttaa caaaatagct cccagaactt cagatcttgc aaaactgctt
     1321 tatctcatat atatatcttt tactttgatg gaattcgctc tgctgagtct cagtaaaatg
     1381 agtctctttg aagctgttat taattctctt ggaagcgtaa gcacggccgg tctccttctt
     1441 catccggacg gcgttgcttt ttatgatagt ttctacgcgg agatcgtcat atctgttttc
     1501 accattcttt cttccgtgaa ctacatgatg tatatataca taataaaaag gaacttcagg
     1561 gatctgaaag acaacgtgga aataaaagtt tttttgggta taatcgcttc tgtaacactt
     1621 ttgataactg caagcctaag gatctcaggc acctatgatt ctttgttcac agccttcagg
     1681 catgcgtttt tccaggtgac ttccatttcc accacttcag gatacaccat tggggatttc
     1741 gatgtttggc ctgccttttc caaggctttg ctgttcactc ttttctttat aggcggctgc
     1801 gcagcggcca ccagcggctc aataaaggtg atccgcataa tcatcatgtt caaacttata
     1861 atgaggggct tttacaaaag actccatcct cttgctgttc gtgccgtaaa aataaagggc
     1921 aaaaatatct ctgcccccat ggtttcatct gttacttcat tcatcatatt atatttcatc
     1981 ttgtatcttt tctctgttgt ggttctttca ttccagggcc ttgatatgga aaccactcta
     2041 agttctcctg caggcctgat gtcaaacata ggtgtcggct tcggtcaagt aagtgaaggt
     2101 gatttcagct gttattcggc tccgctaaag ctttatatgt ccctgctgat ggtggtgggc
     2161 cgtcttgaaa tctttaccgt ttttctgctt ttcgttcctt ctttttggaa ccccaacaga
     2221 tccaataccc gttgatcaga tcatatcaaa atttatttcc ctgtcttcaa tacttacaga
     2281 tcttactctt atatctattg tatcgccaag ggaataggtt ttcccctttc ttcttccgca
     2341 taccctgtgt cccgcgggat catattcata ataatcatcg aaaagctgcg agagctttat
     2401 catgccttca acggtatttt caagttgtac atatatgccg taatttgtaa ctccgcttat
     2461 tataccgctt ttttcctctc ccacatgata agacatgtac tccgcttttt tcatcttctc
     2521 cgcttcccgc tcaagttcta ttgcctgtct ttcctttgca gatgagatct ccgccgcttc
     2581 tttggtcaaa cgcttgtatg ctctggtttt ttttgctgca acttttgcat cgggcccggc
     2641 tgttctttca gccgaagaca taagttcccc ctttagccag ctttttatta ttctgtgtat
     2701 catcagatcc gggtaacgtc ttatgggcga agtgaaatga cagtagtatt taagagccag
     2761 gccgaaatgt cccagacatt gggtattata ttctgccttt tgcatggatc tgagcataac
     2821 ggtactgata atgttttcat ttgcacctcc ggaaatgctt tttaatatcc gggaaagggt
     2881 ctttggcttt atgtcctttg tccctccttc cgggtatatt ccgaatcctt ttaagaacaa
     2941 catcagttct gtaatattgt ccggagaagg tttttcatgg atcctgtaaa caaaggggat
     3001 ttttaaaagt gcaaaatgct ctgcgatcac ctcatttgcc tttaacatga attcctctat
     3061 cagcttattg ccgctccttc tttcgcaggt gctgacctcc accggtatac cttctttatt
     3121 aagggttatg tgtgcttctt caaggtcaaa gtccaagctg cctctcatat ctcttctttg
     3181 ctcaagaatt tttgccagtt catgcatttt tactatgctg tcatacactc cggatggacc
     3241 gctgtcatac ttttttataa gctctctgct gtttttttcc agcatatcgg agacatccgt
     3301 gtataccatt ctggccttgg aattgataat gctttcgtag atttcgtgcc ttatcacttc
     3361 ccctgctttg tctatttcca tatccacaga aatgcaaagt ctgtcttcaa ggggattcag
     3421 gctgcatatt ccgttggaaa gttcggcagg aagcataggc accacctggt ctatgacata
     3481 tatacttgtg cctcttttca gagcctcttt atcaagtgca ctgccttctt ttacatagct
     3541 gctcacatct gcaatatgga ctcccagaag gtaattcccg ttttccagca tatctatcga
     3601 tactgcatca tcgaaatcct ttgaatcggc accgtcgatg gtgaaaatca tcttatcttt
     3661 aagatctctt ctttcctctt tatcttcttc tgtgatacct tttgcgaaca gtttttttgc
     3721 ttcactggct gcttttgaag gaaattcttc tgtaatgcca tactgtctta tcagagatct
     3781 tatatctcct cccggctgac cgctcctgct tattatctgt tttatcctgc cctgggcact
     3841 atctgttcgg ctgggatatt ttattatctc ggcaacgacc ttgtctcctc tttccgcaca
     3901 gtaaaaatcc tttttcttta caaaaatatc ttcatttagt ctcctgtcat ccgggatcac
     3961 aaagccaaac ctgccgtttt tttcaaaggt gcctacgact tcggaaacag aacgggaaag
     4021 tatcccggca actattcctt ccggagaatt cttccaaaga cgggcaggaa taagctcgac
     4081 ttccaccagg tcgccgttca tggcgccatt catgtctttg ccggaaataa atatatcttt
     4141 ctcttctttt attccgcttg cagtttccgt tatcacaaaa ccaaagcctc ttttatgttt
     4201 ctgtaatgta cctgtcactc ttttcatgtt ttttcttttc ttcattcctt tttacctctt
     4261 tttcatatct ttggtttgta tggattatac gtcttctgca acaactatgc aaatccacat
     4321 cacatattta ccttgcatag tcaatgtaag caggtctctt ataaaaaagc aggccctcaa
     4381 aatgcacggg cctgccaggt tattcttgtt atgactgata tcaattatca gatcagtcct
     4441 cccacagcta caaataccgg tgcaaataca acggatacta tagtcatgag cttgataagg
     4501 atgtttatcg aagggcccga tgtatccttg aaaggatctc ccacggtgtc tccaactaca
     4561 ccggctttgt gggcttcgct gcccttgccg ccatgatgtc cctcttcaat atattttttt
     4621 gcattgtccc atgctcctcc tgcattggcc atcattattg ccaccagtac accggctgtc
     4681 agggatcctg ccagcattcc gcccaaagcc tgggtgccca gcaccagccc cacagccagg
     4741 ggtgcgatta tggccaaaag gcccggcgct accatctcct tcagtgcggc ctttgttgat
     4801 atgtcaacac accttgcata atcgggtctt gatgttcctt ccattatgcc ggggtcattc
     4861 ctgaactggg tccttacttc ttctatcatc tggtgtgctg ccttgcctac cgagttcatg
     4921 gtcattgcag agaacaggaa aggaagcatc ccaccaatga acagacccac tataactatc
     4981 ggttccaaca ggcttatcac tttaagatct gtaacagtag cataagctgc aaacagggcc
     5041 aaagctgtca gtgcggcaga gcctattgca aaaccttttc ctatggctgc cgtagtgttt
     5101 cctactgaat ccagtttgtc tgttatacct cttacatctt cgggaagatc tgccatttcg
     5161 gctatgcctc cggcattatc cgaaaccggt ccgtatgcgt cgacagccac tgtcatacct
     5221 gtcgttgaaa gcatccctac tgccgccagt gcgatgccgt aaagccctgc tgcttcatag
     5281 gctatatata tgcccgctgc gatgaacatt atcggccata cggtagaatg catgc
//
LOCUS       DAONHK010000072         5314 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_11977_length_5314_cov_3.927553, whole genome shotgun sequence.
ACCESSION   DAONHK010000072 DAONHK010000000
VERSION     DAONHK010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5314
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11977_length_5314_cov_3.927553"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1764)
                     /gene="pknB"
                     /locus_tag="PLM92_03710"
     CDS             complement(<1..1764)
                     /gene="pknB"
                     /locus_tag="PLM92_03710"
                     /inference="COORDINATES: protein motif:HMM:NF033483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HPR24280.1"
                     /translation="MSSKVIAGRYELLEKIGDGGMAVVYKAKCRLLNRFVAVKILKPE
                     FVKDAKFVENFRKESHAAASLSHPNIVNVYDVGREGSINYIVMELVEGKTLSSVIKEE
                     APLPYKRVIELTKQIASGLSAAHKNNIIHRDVKPHNILLTEDGVAKITDFGIAKAVTS
                     TTIVGNTSEAIMGSVHYFSPEQARGGYVDEKSDIYSLGIVMYEMLTGRVPFDGDNPVS
                     VALMHINNDITPPSGIVPGIPPRLEQIVMKATDKYQPNRFSSAEEMVDQLNNIEFVTR
                     IVGNSAIEPERRRYEEENDEVAEQLRKKVTDSGKKNGNRKKRMIIIIAIAVAVIGGLV
                     AAGFAMGILGKADIKVPDLRGMTYEEAEYKLDELGLRIEEGDLVYSSKYEVGEVVSSD
                     PEKGTMVKKRSVVTVNICKGAEEGTVPNLIGKKQKEAKQLIKKYGFKVGNITTKSGSE
                     EEGTVIEQDPGAGEETKPGSYINLVLSDGSEKDKAEVPSLTGKSLDAAKQAIIDAGFK
                     VGSVSYSNSDSYGKGQVMWQQYEPSTKVKKGSSINIKVSKGSGTSTIAVDISYSKAKQ
                     EIFYLTVTVSDSNGTRTVISGE"
     gene            complement(1754..2506)
                     /locus_tag="PLM92_03715"
     CDS             complement(1754..2506)
                     /locus_tag="PLM92_03715"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HPR24281.1"
                     /translation="MIQIGFKSSKGIKRKNNEDACFVMPKEEVYIVADGVGGNNAGEL
                     ASRTAVTSVAEHIKKNSLAEMFSEEQISEYFKDCMEEANKNVYDLAEKYEQNKGMATT
                     LVVCHMKGNRAYISNVGDSRAYIFSKGTLSQLTEDHTYVNSLIKMGIITEEEAKGHQK
                     VNMITKALGAEKEIAPDFYSTDLDKGDIILLCTDGLYGELDNNKMEKIIKKEENMPVL
                     TDELIKAANKAGGRDNITVICIKIDGGENNEQ"
     gene            complement(2525..3820)
                     /gene="rsmB"
                     /locus_tag="PLM92_03720"
     CDS             complement(2525..3820)
                     /gene="rsmB"
                     /locus_tag="PLM92_03720"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: protein motif:HMM:NF011494.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HPR24282.1"
                     /translation="MDANRKTAYNTLIDVETKKSYSNIALNHQIICGKPDNASFVREI
                     VYGVLENKMLLDYVIDSLVPSGAAKIKTSDLTILRMGIYQLAKMDSVPEYAAVNESVI
                     LAKKFSRGRENFINGVLRSYIRDKYKIKLPDRNEDEIKYLSVKYSYEPWIIKLWLESF
                     DIGFVEELLKAGNESPDVTIRMNWLKVMKEDLVKNLEKNGYTVTEGRHSKNAFHVKGK
                     NLLKTEFYKNGMFSVQDEASQMVAQILDPRHGETIMDVCAAPGGKTLAIAERMNNKGI
                     VLASDIYRRKLDIINRETERLGINIVETRTWDATKVDSSMAGKADRVIVDAPCSALGV
                     IRRKPEIKYKKETAEIQALPGKQLAILSASSSYVKPGGKLIYCTCTINPYENERVISD
                     FIRKVPSFIVEETVQFLPNVDDTDGFYICLMKKTDDLFE"
     gene            complement(3822..4526)
                     /locus_tag="PLM92_03725"
     CDS             complement(3822..4526)
                     /locus_tag="PLM92_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010965031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HPR24283.1"
                     /translation="MYFGMFDPTFILLVPAIIFAFIAQSRMNSAYNNYSGVAGRNGIT
                     GAAAARKILDSNGLDHISVEMGKGRLSDHYDPRKKKMRLSPDVYEGTSIASLAIAAHE
                     SGHAIQHGSKYVPLSIRNVIALPVSIVSGLSWPLLIIGLIVIQTGYYSQGNLIFNIGI
                     FAFVGVVLFHTITLPVEFNASRRALVQLEELGIVLPEETGGAKKVLSAAAMTYVAALA
                     VAVANLLRILLIRGRN"
     gene            complement(4560..>5314)
                     /gene="fmt"
                     /locus_tag="PLM92_03730"
     CDS             complement(4560..>5314)
                     /gene="fmt"
                     /locus_tag="PLM92_03730"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HPR24284.1"
                     /translation="PENITTDRVFFDKLKNTSPNLIVVAAYGKILPEEIIHMPGCGCV
                     NIHASLLPRFRGAAPIQRAILAGERYIGVTIMCMEEGLDTGDILSAAETEIDRKTSGQ
                     LHDELAVMGAKLLIETIISMEKGDIVKKPQNDSLSTYAPMIHKKEAEIDFSKDPSEIE
                     RIIRAFDPWPGAYTFHKGEMMKIWKAEAFDEEDCGGKRSGTVISVSEKGIGIAAGKGL
                     LLVGEMQMPGRKKMPVKDFLRGNSIEIMTVLG"
BASE COUNT         1199 a         1313 c          946 g         1856 t
ORIGIN      
        1 ctctcccgaa ataactgttc ttgttccgtt gctgtccgaa accgttactg tcaggtagaa
       61 tatctcctgc tttgcttttg aataacttat atcgactgct atggttgaag ttccgcttcc
      121 cttgctgact tttatgttta tggaagatcc tttttttact tttgtgcttg gttcatactg
      181 ctgccacatc acttggcctt ttccgtatga atcactgttg ctgtatgaaa cactgcccac
      241 cttgaagccc gcatctatta tggcctgctt tgcggcatca agacttttac ctgtgagaga
      301 aggcacttcc gctttgtctt tttccgaccc gtcactcaag acaagattta tatatgagcc
      361 gggtttggtt tcctcgcctg ctccgggatc ctgttctata actgtccctt cttcttcact
      421 gcccgatttg gtggttatgt taccgacttt gaatccgtat ttttttatga gttgtttcgc
      481 ttctttttgt tttttgccta tgagattggg cactgtgccc tcttccgccc ctttacatat
      541 gttaactgtt accacggatc tcttcttgac cattgttcct ttttcgggat ctgaagaaac
      601 aacttctccg acctcgtatt tcgaactgta caccagatcc ccctcttcga tcctgagtcc
      661 cagttcgtcc agtttgtatt ccgcctcttc atatgtcatc cctctgagat ccggtacttt
      721 tatatctgct ttgcctaaaa tccccattgc gaatcccgct gccaccagtc cgccgattac
      781 tgctacggct atggcgatta ttattatcat tctttttttc ctgtttccgt tctttttgcc
      841 ggaatctgtc actttttttc tcagctgttc cgcaacttca tcattctctt cttcatatct
      901 ccttctttcg ggctcaattg ctgaattccc gactatacgc gtaacgaact ctatattgtt
      961 gagctggtca accatttctt ccgctgacga aaatctgttg ggctgatact tatccgtggc
     1021 tttcattact atctgttcaa gtctgggagg tattccggga actatcccgg atggaggtgt
     1081 gatatcgttg tttatatgca taagcgcaac gcttaccgga ttgtctccgt caaaggggac
     1141 tcttcccgtc agcatttcat acataactat tcccagggaa tagatgtctg atttttcatc
     1201 cacatacccc cctcttgcct gttcaggcga aaaataatgg acagacccca ttattgcttc
     1261 cgatgtattc ccaactattg tggtggaagt aacagctttt gcaataccaa aatcggttat
     1321 ttttgcgaca ccgtcttctg taagaagaat gttgtgaggc ttgacatccc tgtgtattat
     1381 attgttttta tgagcagcgg aaagccctga tgcgatttgc tttgtcagtt ctataactct
     1441 tttgtaagga agcggagcct cttcttttat gacgctgctc aaagtttttc cttcaaccag
     1501 ttccataacg atataattga tgcttccttc ccgtcccaca tcatacacat ttactatatt
     1561 gggatgcgaa aggctggcag cggcatgaga ttcttttctg aagttttcca caaacttggc
     1621 atccttcacg aattcaggct tgagtatctt gaccgctaca aaacggttaa gcagtctgca
     1681 tttcgctttg tagacaacag ccatacctcc gtcacctatt ttttcaagca attcatatct
     1741 tcctgcaatg accttactgc tcattatttt ctcctccatc gatttttata catatcactg
     1801 ttatgttatc tcttcctccg gctttgttgg cggcttttat cagttcatcg gtcaagaccg
     1861 gcatattctc ttctttcttt attatctttt ccatcttatt attgtccaat tctccgtaaa
     1921 gcccgtccgt gcaaaggagt ataatgtctc ctttatcaag atccgtgctg taaaaatccg
     1981 gagctatttc tttttctgct cccaaagcct ttgttatcat attgactttc tgatgtccct
     2041 tggcttcttc ttcggttatt atccccatct ttatcagcga attcacgtat gtgtggtcct
     2101 cggtcagctg ggaaagagtc ccttttgaaa aaatataagc tctgctgtct cccacatttg
     2161 agatatatgc tctgttaccc ttcatatggc atacgaccag cgttgtggcc attcctttgt
     2221 tttgctcata tttttcggcc agatcataaa catttttatt tgcttcttcc atgcagtctt
     2281 taaaatactc actgatctgt tcctcagaaa acatttctgc aagagaattt tttttgatgt
     2341 gttcggccac agaggtcaca gctgttctgc tggccagttc gccggcatta ttgccaccca
     2401 caccatccgc aacgatgtat acttcttctt ttggcataac aaaacaggca tcttcattat
     2461 tctttctttt tattcctttg ctgcttttga atcctatttg tatcatgctt tttcttttcc
     2521 ttttttactc aaaaagatcg tcggtttttt tcatcagaca aatataaaat ccatcggtat
     2581 catccacatt gggcaggaat tgaactgttt cttccactat gaaagacggc acttttctta
     2641 taaaatcact tatgactctt tcgttttcat agggattgat cgtgcatgta caataaataa
     2701 gcttcccgcc cggttttaca taacttgaag atgcggataa aatggccagc tgcttcccgg
     2761 gcagtgcctg tatctctgca gtttcttttt tgtattttat ctccggtttt ctcctgatca
     2821 caccaagcgc cgagcagggt gcatccacaa tcactctgtc cgcttttccc gccatggaag
     2881 aatcgacttt tgtggcatcc catgttcttg tctctacgat attgatcccc agtctttctg
     2941 tttctctgtt tattatatca agctttctcc tgtaaatatc agaggccaga acgatacctt
     3001 tgttattcat cctttctgcg atggcaagag tctttcctcc cggggccgcg catacatcca
     3061 ttattgtttc cccgtgtctt ggatcaagta tctgggccac catctgtgat gcttcgtcct
     3121 gcacggaaaa cataccgttc ttgtagaact cagtcttgag cagattcttt ccttttacat
     3181 gaaatgcatt tttgctatgc ctgccttctg tcacagtgta tccgtttttt tccaggtttt
     3241 ttaccagatc ctctttcatt accttcagcc agttcattct tattgtcaca tccggacttt
     3301 catttccggc tttgagcaac tcttcaacaa atccgatatc aaaactttca agccagagtt
     3361 ttatgatcca tggttcatat gaatatttca cagaaagata ttttatctca tcttcatttc
     3421 tgtcgggaag ttttatttta tatttatctc ttatataaga tcttaataca ccgtttataa
     3481 agttttctct gccccttgaa aatttctttg ccagtataac actttcgttt acagctgcat
     3541 actccggcac cgagtccatt ttggccagct ggtatatgcc catcctcagt atagtaaggt
     3601 cgctggtctt gatttttgcg gctccactgg gcaccaggct gtctattaca tagtcaagca
     3661 gcattttgtt ttcaagaacg ccgtatacaa tctctctgac gaaggaggca ttatccggtt
     3721 tcccgcatat tatctgatga ttaagtgcta tattcgagta ggattttttt gtttccacat
     3781 caataagtgt gttgtatgct gtttttctgt ttgcgtccat tctaattacg cccccttatc
     3841 aacaatattc ttaatagatt cgccactgct acagccaggg ctgccacata cgtcatagct
     3901 gccgccgaaa ggaccttttt ggctccgcct gtttcttcgg gcaaaactat acccagttct
     3961 tccagctgaa caagtgctct tctgctggca ttgaattcca caggcagcgt tatagtatga
     4021 aaaagtacaa ctccaacgaa agcaaatatt cctatattaa aaatcaggtt tccctgggaa
     4081 taataacctg tctgtatcac tatcagccct attatcaaaa gaggccagga aagcccggat
     4141 acgatgctta ccggaagggc aatcacgttc cttatactaa gaggtacata ttttgaacca
     4201 tgctgtatcg catggcccga ctcatgagcc gctattgcca gagaagcaat ggaagtgccc
     4261 tcatacacat cgggtgaaag acgcattttc tttttgcgcg ggtcataatg gtctgataat
     4321 cttccttttc ccatttccac cgaaatatga tcaagtccgt tggaatcaag tatctttctt
     4381 gctgcagcgg ctcctgttat gccatttctt ccggctaccc ctgagtaatt gttataagcg
     4441 gaattcatcc gactctgtgc tataaatgca aaaataatgg ccggtaccag cagtataaaa
     4501 gtcggatcaa acattccgaa atacatatgt tttttacctc caaaacacca agttacattt
     4561 taacccaaaa ccgtcattat ttcaatgctg ttacctctca aaaaatcctt tacgggcatt
     4621 tttttcctgc cgggcatctg catttcccct acaaggagca gtccttttcc cgcggcgata
     4681 cctattcctt tttcggaaac actaatcacc gttccggatc ttttcccacc acagtcttct
     4741 tcgtcaaatg cttctgcctt ccatattttc atcatttcac ctttatggaa ggtgtaggca
     4801 ccgggccagg gatcgaaagc tctaatgatt ctttctattt cagagggatc tttggaaaaa
     4861 tctatttccg cttctttttt atgtatcata ggtgcatatg tggaaagact gtcattttgg
     4921 ggttttttga ctatatctcc tttttccatt gaaattattg tttctataag aagctttgcc
     4981 cccataacgg ccagctcatc atgtagctgt cccgaagttt tacggtcgat ctctgtttcc
     5041 gctgcagaaa gtatatctcc cgtgtcaagt ccttcttcca tacacattat ggtgacacct
     5101 atataccgtt ctccggcaag tattgctctc tgtatgggtg ctgcccccct gaatctgggc
     5161 aaaagggatg catgtatatt gacacatccg catcccggca tatgaatgat ctcttccggc
     5221 aggatctttc cgtatgccgc caccacgatc agattcggag atgtattctt caatttatca
     5281 aaaaaaaccc ggtctgtcgt tatgttttcg ggtt
//
LOCUS       DAONHK010000073         5307 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_11999_length_5307_cov_4.612909, whole genome shotgun sequence.
ACCESSION   DAONHK010000073 DAONHK010000000
VERSION     DAONHK010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5307
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11999_length_5307_cov_4.612909"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..700
                     /locus_tag="PLM92_03735"
     CDS             <1..700
                     /locus_tag="PLM92_03735"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF013168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_function: GO:0016860 - intramolecular oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]
                     imidazole-4-carboxamide isomerase"
                     /protein_id="HPR24285.1"
                     /translation="DKETVYNEDPCLVAKEFRRAGAEYLHIVDLDGADKGKPVNFEIV
                     SKIAEETDMFIQVGGGIRNEERIKKHLSCGVNRVILGTVAAENPEFVREMVRKYKDKI
                     AVGVDVRDGKVAVNAWKEVTNIDGMEFCRKLRDMGVKTVIYTDTSKDGGLAGTNMEAY
                     RELKKIEELDIIAAGGIRYERELTALRGMVKGAILGKALYSDKLSLKRCLELVGSNVG
                     MDGIEKKEINWKGH"
     gene            700..1455
                     /gene="hisF"
                     /locus_tag="PLM92_03740"
     CDS             700..1455
                     /gene="hisF"
                     /locus_tag="PLM92_03740"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015537578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HPR24286.1"
                     /translation="MISKRIIPCLDVRDGKVVKGRKFDSIKEVADPVSMAAFYNEAGA
                     DELVFYDITASFEKRKLFNEILKEVASKIFIPLTVGGGIDSLEDFDTVLKCGADKVSV
                     NSGAIKTPGIIDAAAKRYGNQCVVLSMDVKRVDDKFIVFSMGGRENTGKEAASWAIEA
                     VSRGAGELVINSIDTDGVKDGFDIEMLSALASKVSVPVVASGGAGKMEDFADLFEKTG
                     VEAGLAASIFHFREVEISDLKKYLKEKGVEVRI"
     gene            1452..2114
                     /gene="hisIE"
                     /locus_tag="PLM92_03745"
     CDS             1452..2114
                     /gene="hisIE"
                     /locus_tag="PLM92_03745"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018450024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HPR24287.1"
                     /translation="MNLKFDEKGLIPAIVQDHKNGQVLTLAYMNKESLEITIKEGRTC
                     FWSRSRNELWRKGAASGNYQEVVGIRADCDKDALVIEVIKNGPACHTGKESCFFNEIF
                     DADRTKTDPERSAENGFSWHALYKTISQRRKNPREGSYTSYLFEMGMEKILKKIGEES
                     TEVIIAAGKKDKEETVYEIADLAYHVMVMMSEMEIEPEEITAELAKRHVIDRKKKQER
                     MG"
     gene            2152..3174
                     /locus_tag="PLM92_03750"
     CDS             2152..3174
                     /locus_tag="PLM92_03750"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HPR24288.1"
                     /translation="MKKQMRSKTKKMPAILALIIAAVLLQAGCGTEEEISDTGFYLDT
                     TCTITLYDINKDEGERLIKKSFDICEKYERLMSKTIKGSDIDRINRAEGSPVSVSPET
                     IKVIKEGMRMGKLSGGKFDITIGRVTELWDFNSDRAEVPADEKIKEAVETVDYRKIQT
                     EGNTITLEDPAAKLDLGGIAKGYIADRITKMLVKNGAEKAIINLGGNVVAIGEKEENT
                     PWNIGVERPYSDRSEVIGVVEAVDNTVVTSGIYERFFEKDGKKYHHILDPETGYPAQS
                     DLEAVTVKGPSGSSMECDAISTVCILIGEKKAKKIIESMDGMEALFINKNNKATPTKG
                     LSFVIQ"
     gene            complement(3146..4342)
                     /locus_tag="PLM92_03755"
     CDS             complement(3146..4342)
                     /locus_tag="PLM92_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007049511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPR24289.1"
                     /translation="MRRKFSELSMRLETLAIFRKLLDDPVIAKLRTLFLLTEESEDER
                     QVISCFSSFISALYEKTPDLSKYIKSLILNDDNLYIRQKSAGKEISPTIESCLYAELE
                     LFQEISQLKVPDIKSAIGYHGFLPEWETSHCDLKGEYKKQIEKLPVKGFGIFAKYRSF
                     SVKDGEFIPILFPDNQSLSDLFGYRKEREQVIKNTEAFLEGKEASDMLLYGDAGTGKS
                     STIKAIVNEYAPKGLRLVEIRKSQIFDIPKVTEKLSGNPLKFIIYIDDLSFSGNNENF
                     SSLKAILEGSAVSRSSNILIYATSNRRHLLKETSAERQGDDLHINETLQETMSLAARF
                     GLTVAFQKPDKDTYLYITKELAKKYGLDMSDEELFKKAEAHAIRTNGRTPRAAKQFIE
                     LQKTGL"
     gene            4439..5212
                     /locus_tag="PLM92_03760"
     CDS             4439..5212
                     /locus_tag="PLM92_03760"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HPR24290.1"
                     /translation="MITLFLQILKSGNYDIILPMREEIKRKLKEKAEKDYKEFNTKLI
                     PGIDKKSVMGVRMPDVRALAKQIAKEDFRKFMDEMDEAENPCYEEKLIYGMVIGYAKI
                     NDDERFDLIRRFVPYIDNWALCDSVDSTYRFIRKDPEKGFDFLKTYLDSDREFEKRFG
                     TVMLLAHYVNNDDINEVYLPKVIEEMKLLGRDQPYYAMMAVAWTLSVCYIKNGKLTVG
                     LLSGDGLDHITRKKTIQKIRESFRVSEEEKENLKKLIKA"
BASE COUNT         1783 a          886 c         1320 g         1318 t
ORIGIN      
        1 tgacaaggag acagtataca atgaagatcc ctgtcttgtg gcaaaagaat tccgccgtgc
       61 aggagctgaa tatctgcata tagtagatct tgacggagcc gacaagggta aacctgtgaa
      121 ctttgaaata gtaagcaaaa tcgcagaaga gacggatatg ttcattcagg tgggaggcgg
      181 aataagaaat gaagagagaa taaaaaagca tctctcctgt ggtgtaaacc gtgtgatact
      241 gggcactgtg gctgccgaaa accctgaatt cgtaagagaa atggtccgga aatacaagga
      301 taaaatcgcg gtaggggttg atgtgcggga tgggaaagtt gctgtaaacg cctggaagga
      361 agtgacgaat atagacggaa tggagttttg cagaaagctt cgtgatatgg gagtaaagac
      421 tgttatatat acggatactt ccaaggatgg ggggcttgca ggtaccaaca tggaagcata
      481 cagagaactg aaaaagatag aagagcttga tataatcgca gccggcggaa taaggtatga
      541 aagggaactg actgctttaa ggggtatggt caaaggagcg atactgggca aagcacttta
      601 cagtgacaag ctttccctga aaagatgtct tgaacttgtg ggaagcaatg tggggatgga
      661 cggaatagaa aaaaaagaaa taaactggaa gggacactga tgataagcaa aagaataata
      721 ccctgtcttg atgtaaggga cggaaaagtc gtaaagggga gaaaattcga cagcataaag
      781 gaagtggccg atcctgtttc gatggcagct ttctacaacg aggcgggagc tgatgagctg
      841 gtattttatg atataaccgc aagttttgag aaacgtaagc tttttaatga gatattgaag
      901 gaagtggctt cgaagatatt catccctctt actgtaggag gcggaataga ttcacttgag
      961 gattttgata cagtattaaa atgcggtgct gataaggtca gcgtcaacag cggtgccata
     1021 aaaacccccg gcattataga tgcggcagcg aaaagatacg gaaatcagtg cgtagtgctt
     1081 tccatggatg tcaaaagagt ggacgataaa ttcatagttt tttccatggg aggccgtgaa
     1141 aacacaggaa aagaggcggc ttcctgggct attgaagcag tttcaagagg ggccggagaa
     1201 ctggtaataa acagtattga cactgatggg gtaaaggacg gtttcgatat agagatgctt
     1261 tctgctttgg cttcaaaggt ttctgtcccg gtggtggctt cggggggggc cgggaaaatg
     1321 gaagatttcg ccgatctttt tgagaagacc ggagtcgagg cgggtcttgc cgccagcata
     1381 ttccatttta gagaagtgga aatatccgat ctgaaaaaat acttaaaaga aaaaggtgtt
     1441 gaggtgcgca tatgaatctt aaatttgatg agaagggact tatacccgcc atagtacagg
     1501 atcataaaaa cgggcaggtg ctcacactgg catatatgaa caaagaaagt cttgaaataa
     1561 ccataaaaga aggaaggacc tgtttttgga gccgcagcag gaatgaactc tggagaaagg
     1621 gcgctgcaag cggcaactat caggaagtgg tcggcatcag ggcggattgt gacaaagatg
     1681 ctctggtcat agaagtcata aaaaacgggc cggcatgtca tacaggaaaa gaaagctgtt
     1741 ttttcaatga aatatttgat gcggacagaa ccaaaacgga tccggaaaga tctgcggaaa
     1801 atggattctc gtggcatgct ctttataaaa caataagtca gcgaagaaaa aatcccagag
     1861 aaggcagtta taccagctat ctttttgaaa tgggtatgga aaagatacta aaaaagatag
     1921 gcgaagaaag tacagaggtc ataatagctg ctggcaaaaa agataaagaa gaaactgttt
     1981 acgaaatcgc tgatctggct tatcatgtaa tggtgatgat gtcagagatg gagatcgaac
     2041 ctgaagaaat aactgcagaa ctggcgaaaa gacatgtaat agacaggaag aagaaacagg
     2101 aaagaatggg ctaggtacca ttctgctgtc gggtgtaaag ggcgataata tatgaaaaaa
     2161 cagatgagat ccaaaacaaa aaaaatgccg gccattttgg ctttgatcat cgctgccgtt
     2221 cttttgcagg caggatgtgg aacagaagag gaaatatcag atacgggatt ttatcttgat
     2281 accacatgca caataaccct ttatgacatc aataaagacg aaggagaaag actaataaaa
     2341 aaatcctttg acatatgcga aaaatatgaa agacttatga gcaaaacaat aaaaggcagt
     2401 gatattgaca ggataaacag ggcagaggga agtcctgttt cagtaagtcc cgaaaccata
     2461 aaagtaataa aggaagggat gagaatgggt aaattgtccg gaggcaaatt tgatataacc
     2521 ataggaagag tgacggaact ctgggatttc aacagcgacc gggcggaagt ccctgctgac
     2581 gaaaagataa aagaagccgt tgaaaccgtt gactacagaa aaatacaaac agaaggaaat
     2641 acaatcactc ttgaagaccc ggcagcaaaa ctggacctgg ggggcatagc caagggctac
     2701 atagctgaca gaataacaaa aatgctggtt aaaaacggag ctgaaaaagc aataataaat
     2761 ctgggaggaa atgtggtcgc tataggagaa aaagaagaaa atactccatg gaacatagga
     2821 gtggaaagac cttacagcga caggagcgaa gtgatcggcg tggttgaagc tgtggataat
     2881 actgttgtca catcaggcat atatgagcgt ttttttgaaa aagacggaaa gaaatatcat
     2941 catatcctgg atccggaaac gggatacccg gcacaaagtg atcttgaagc tgtgacggtg
     3001 aaaggacctt cgggcagttc catggaatgt gatgcaatca gtacagtgtg catactgata
     3061 ggcgaaaaaa aagcaaaaaa aattattgaa agcatggatg gtatggaggc tctttttata
     3121 aacaaaaata acaaggcaac gccgactaaa ggcctgtctt ttgtaattca ataaactgct
     3181 ttgcagctct cggagtacga ccgtttgttc ttatggcatg agcttcggcc tttttgaaaa
     3241 gctcttcgtc ggacatatca aggccgtatt tttttgcaag ttcttttgtt atataaagat
     3301 aagtatcttt gtcgggcttc tggaaagcaa cggtaagacc gaacctggca gcaagactca
     3361 ttgtttcctg cagggtctcg tttatgtgaa gatcatcccc ctgtctttcg gcggaagttt
     3421 cttttaggag gtggcgtctg ttactggtgg catatattag aatattactg cttcttgaaa
     3481 ccgcacttcc ctccaaaata gccttaagcg aggaaaaatt ttcgttgttg ccggaaaaac
     3541 tgagatcatc tatatatatt atgaatttaa gaggatttcc ggaaagtttt tcggtgactt
     3601 tggggatatc aaaaatctga cttttcctta tttccaccag ccgcagcccc ttgggcgcat
     3661 attcatttac gatcgctttt attgttgagc ttttgcctgt accggcatca ccgtagagaa
     3721 gcatgtcact ggcttctttg ccttcaagaa aagcctcggt attttttatt acctgttctc
     3781 tttctttgcg gtaaccgaaa agatccgaaa gagactgatt gtcggggaac aatatgggaa
     3841 taaattctcc gtcttttaca gaaaaagatc tgtatttggc aaaaatgccg aatcctttaa
     3901 cgggaagttt ttcgatttgt tttttatatt ctcctttgag atcacaatgg gaggtttccc
     3961 attccggcag aaaaccgtga taacctatgg cggatttgat atccgggacc ttgagctggg
     4021 aaatttcctg gaacagttcc agctcggcat aaagacagct ctcaatggta ggcgagatct
     4081 ctttcccggc ggatttttgt cttatataaa gattgtcgtc attaaggata agggatttta
     4141 tgtatttgct cagatcaggg gttttttcgt acagagcgga aataaaagag gaaaaacatg
     4201 aaatcacctg tctttcatcc tcgctttctt ctgtcagcaa aaataatgtc ctgagttttg
     4261 caataacagg atcatccaga agttttctga atatggccag ggtctccagc ctcatgctca
     4321 gttccgaaaa ttttcttctc atctaatcaa atccttcatt aaatcctttt atgttttagc
     4381 tgtatatagc aattgccgag tctttttatg aagcgctttg gcatatgata acgatattat
     4441 gataacactt tttttgcaaa tactcaaatc gggaaactat gatataatat tgcccatgag
     4501 agaagaaata aagagaaaac taaaagaaaa agccgaaaag gattataagg aattcaacac
     4561 aaaactcata cccgggatag ataagaaaag tgtcatggga gtaaggatgc cagatgtcag
     4621 agcactggca aaacagatcg ccaaagaaga cttcagaaaa ttcatggatg agatggatga
     4681 ggcggaaaat ccctgctatg aagaaaaact tatttacggg atggtaatag ggtatgcaaa
     4741 gataaatgat gatgagcgtt ttgatctgat caggcggttt gtcccatata tagataactg
     4801 ggcattatgt gacagtgtgg acagcacata cagattcata agaaaagacc ccgaaaaagg
     4861 gtttgatttt ttgaagacat atcttgattc ggatagagaa ttcgaaaaaa gattcgggac
     4921 ggtcatgctt cttgcacact atgtaaacaa tgatgatata aatgaagttt atctgccaaa
     4981 agtcatagaa gagatgaaac ttctgggcag ggatcagcct tattacgcaa tgatggctgt
     5041 tgcctggaca ctttctgtgt gctatataaa aaacggaaaa ctgactgttg ggctgctttc
     5101 cggtgacggc ttggaccata taacccgcaa aaaaaccatt caaaagataa gggaatcttt
     5161 cagagtatcg gaagaagaaa aggagaacct gaaaaaactc ataaaggcat aaagtaataa
     5221 tagcgaagaa acagggcaat gataagagga acagggtctg taaagtagat atgaaaaaaa
     5281 gaggccatga gaataatgtt tctgcta
//
LOCUS       DAONHK010000074         5252 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_12180_length_5252_cov_4.128921, whole genome shotgun sequence.
ACCESSION   DAONHK010000074 DAONHK010000000
VERSION     DAONHK010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5252
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12180_length_5252_cov_4.128921"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..867
                     /locus_tag="PLM92_03765"
     CDS             <1..867
                     /locus_tag="PLM92_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006000614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyglutaryl-CoA dehydratase"
                     /protein_id="HPR24291.1"
                     /translation="LEKNPGFRFSLGLAKRGLMGFIYGDLFMRMLYAVRPYESYPGSA
                     NALFEKWSRIALRNVTSGSIVRFRMIIKKMVKEFDELPIKENLKKPKVGVVGEILVKY
                     HPTANNDIVDVIEKEGGEAVVLDLIDFFLYGMYSKQFNYEQLSGSYKQMMVNKAAISF
                     IESIRKPARKALAESKRFEEPLHIEEIAAKAQEIISLGNQCGEGWLLTGEMVDLIDSG
                     VENIVCLQPFACLPNHVTGKGMMKALRKRNPYANIAAIDYDPGASDVNQLNRIKLMMA
                     TAYKNLQKKEEC"
     gene            927..1649
                     /locus_tag="PLM92_03770"
     CDS             927..1649
                     /locus_tag="PLM92_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006000616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPR24292.1"
                     /translation="MGRHGTIAGRKAAQDSKRSAIFTKYSRAITVAAKNGGDPEYNFA
                     LKHAIDMARSINMPNDNINKAVKRGTGELEGVSYETGSFEGYGASGVAVIVDVLTDNK
                     NRTTSFIKSAFDKYGGNLGTPGCVSYMFSRHGLIIIEKTDDTDEDIIMETALEAGMED
                     MKTHEDSYEIITDPDDFPNVKQALQDAGYEFVEADIEYLPSTEATPPEEELGKLKKMV
                     EALENNDDVQKVYHNCSIDLEQ"
     gene            1686..1863
                     /gene="ssrS"
                     /locus_tag="PLM92_03775"
     ncRNA           1686..1863
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PLM92_03775"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            2059..3726
                     /locus_tag="PLM92_03780"
     CDS             2059..3726
                     /locus_tag="PLM92_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005585075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT/YSL family transporter"
                     /protein_id="HPR24293.1"
                     /translation="MSKNQNGVKHAGSFKEQFTARGMMIGVIGAIILTMSSMFVALKI
                     SSLPWPIMFVALLSMFALKALGGTNINEINVTHTAMSAGAMVAGGLAFTLPGIWMLDP
                     EEDINAVILIVITLGGVVLGLIFTALIRKYFIVTKELPYPMGQAAAEALITGDQGGKK
                     AGVLFGSLGAAAVFTALRDWFLVIPAMLPANMSQKMLAFGSFGAIWLSPMLISVGYII
                     GPVFIGVWFLGALIGDFGILIGGQSFGWWDMETAAAIKSSLGIGLMVGTGIGIIIKGI
                     LPKSKEIFGAMFKKDTLGDSIVKMRWAPIAMVILAFLFTIIADMGVIASVITILGVWV
                     ATAMSAQCVGQSGINPMEIFGIIVLIVAKAASAAGSLAGIGEIAAFFVAAVVAVACGL
                     VGDIMNDFKAGYILKSDPKAQWYAECIGSLTGAFVAVVVLLAIVKAYGGEVFGSEVFP
                     AAQAAAVSAMVGGISHMTAFLLGMAIAVVLYCFDFPVMTLGLGVYLPFYLSATAFVGG
                     ALRFILDRAAPEWSKRGMGQVIAAGVLGGEAITGVVIAIIIAVRMVL"
     gene            3746..4708
                     /locus_tag="PLM92_03785"
     CDS             3746..4708
                     /locus_tag="PLM92_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005884932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HPR24294.1"
                     /translation="MKEIKITDIKGIKVGNAQSIRGGTGCTAIICEAGAFAGVDVRGG
                     APASRETELLKPVNTVKQIHCVMLSGGSAFGLAACDGAMQYLEEKKIGFDVGIGMVPI
                     VCGASLFDLVLGDPKTRPDKEMGYEACKAAENGEPAQGNEGAGTGASVGKFLGTERMM
                     KSGLGIYAVQLGKIKCGAVAAVNSLGDIVDYDTKKVIAGILSEDRKSLSCTKKIMYQE
                     IERPRNVFTGNTTVGCVVTNANLDKDQACKLASIAHNGYARTIEPVHTAADGDTVFVM
                     STGEEKAAFDPLSALVSEVTGRAVNNAVKNAKPAYGLPAACDFR"
     gene            4752..5174
                     /locus_tag="PLM92_03790"
     CDS             4752..5174
                     /locus_tag="PLM92_03790"
                     /inference="COORDINATES: protein motif:HMM:NF019500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HPR24295.1"
                     /translation="MKKLKMLKAIMKMTHADKLLISYVVFLVIGSFLIMLTDPTINTF
                     GNALWYCYTMGVTIGFGDLVVTTVFAKVISVLISLYTILIVGMIPAIVVSYYLEVVKL
                     REKETSTHFLYKLEHLSELSKEELEELSEKVKKFQKKQ"
BASE COUNT         1614 a         1002 c         1339 g         1297 t
ORIGIN      
        1 cttgaaaaaa atccgggatt caggttttct ctgggtctgg caaaaagagg gctaatggga
       61 ttcatctacg gagacctttt catgcgcatg ctttatgccg taagacctta tgaatcatat
      121 cccgggtctg ccaatgccct tttcgaaaaa tggtcaagga tagccctaag aaacgtgacc
      181 agcggcagca tcgtaaggtt caggatgata ataaaaaaaa tggtaaaaga attcgatgaa
      241 ctgcccataa aagaaaatct gaaaaaaccg aaggtcggcg ttgtgggaga gatactggta
      301 aaatatcatc ccactgccaa caatgatata gtcgacgtaa ttgaaaaaga aggcggtgaa
      361 gcggtagtcc ttgacctgat agacttcttc ctttacggta tgtacagtaa gcagttcaat
      421 tatgaacagc tttccggctc gtacaaacag atgatggtca acaaggcggc cataagtttc
      481 attgaatcaa taagaaaacc tgccagaaaa gctcttgcgg aaagcaaaag atttgaagag
      541 ccgctgcata ttgaagagat agccgcaaaa gcccaggaaa taatttctct ggggaaccag
      601 tgtggagaag gctggctcct tacgggagag atggtggatc ttatagacag tggagtcgaa
      661 aatatcgtat gtcttcagcc ttttgcatgt ctgccaaatc atgttacagg caaaggaatg
      721 atgaaagccc tgagaaagag aaatccttat gccaatatag cagcaataga ttatgacccg
      781 ggagccagcg atgtaaatca gttgaacagg ataaaactga tgatggctac ggcctacaag
      841 aaccttcaaa aaaaagagga atgttgaata ttatgagata tcattgtata atatcccggt
      901 aaaacataca gttaggaggt gtctttatgg gaagacacgg aaccatagcc ggcagaaaag
      961 ccgcacagga ttcaaaaaga tctgcgattt ttactaagta ctcaagagcc ataacagtgg
     1021 cggcaaagaa tggcggagat cccgaatata attttgccct taaacatgcc atagatatgg
     1081 ccagatccat caacatgccc aatgacaata taaacaaggc tgtaaaaagg ggcacaggcg
     1141 agcttgaggg agtaagctac gaaacaggaa gctttgaggg atacggagct tcgggagtgg
     1201 ccgttatagt agatgtcctt acagacaaca agaacaggac cacatctttc ataaaaagcg
     1261 cttttgacaa atacggaggc aacctgggaa caccgggatg tgtgtcctat atgttttcaa
     1321 gacacggcct tataataatc gaaaaaactg atgataccga tgaagatatt ataatggaaa
     1381 cggctctgga ggcaggtatg gaagatatga agacccatga agacagttat gagataatta
     1441 cagatccgga tgatttccca aatgtaaaac aggcattgca ggacgcagga tatgaatttg
     1501 ttgaagcaga tatagaatat cttccttcca cagaggccac gccgccggaa gaggaacttg
     1561 ggaaactcaa aaaaatggta gaagcgcttg aaaataatga tgacgttcaa aaagtttatc
     1621 ataactgcag cattgacttg gaacaataaa aaaagtatgt tataatctat tcaaccaaaa
     1681 gatatgattt tctgggttgt acgttaagac tgtataattc aacctacatt atagcgcagg
     1741 gagcccgtat atccggcgcc tatgtcaggc ctcccaagag aggaagacag aagcacggac
     1801 gagagcaccc accttaggaa actagggcgt gaattgacgg tctgacggca agccggaata
     1861 gaaaacacgg cggtcgggtt tgttcacggc cgctttttat ttgcctgttt tattgcaaat
     1921 atgcggtttt ttttgtataa tggcccagta ccaaagcaga aaaatgttta tcgtttttcg
     1981 gcattgaaaa caaaaaatat tacttgttta agaccccggc cactgatacc gggggtgcat
     2041 tttgcacgag gaggtagaat gtcaaaaaat cagaacggag ttaaacatgc cggctcattc
     2101 aaagagcaat tcacggcaag agggatgatg ataggtgtga taggagccat aatactgaca
     2161 atgagttcca tgttcgttgc cttgaagatc agctcactgc cgtggcccat catgtttgtt
     2221 gcgctgcttt caatgtttgc attaaaagca ttggggggca ccaatatcaa tgagataaat
     2281 gtgacccata cagcgatgtc agcaggagcc atggtggccg gaggacttgc ttttacactg
     2341 cccggcatat ggatgcttga cccggaagaa gatatcaatg cagttatcct catagtgatc
     2401 acactgggag gagtagtgct tggcctgata ttcacagcgt taataagaaa gtattttata
     2461 gttacaaagg agcttcctta tcccatggga caggcagcag cagaagccct tatcacagga
     2521 gatcagggag ggaaaaaagc cggagtgctt tttggctctc ttggagcagc agcagtattt
     2581 acggctctca gagactggtt tttggtgatc ccggccatgt tgccggctaa tatgagccag
     2641 aaaatgctgg catttggatc attcggagcg atctggcttt caccaatgct catttcggtg
     2701 ggatacataa tcggacctgt ttttataggc gtatggttcc tgggagcttt gataggagat
     2761 ttcggtattc tcatcggcgg acagagtttc ggatggtggg atatggaaac ggcagcagcc
     2821 ataaaatctt ccctggggat aggtcttatg gtgggaacag gtattggcat aataataaaa
     2881 ggaatattgc ccaaatcaaa agaaatattc ggagccatgt tcaaaaaaga cactttgggt
     2941 gattcaatag taaaaatgag atgggctccc atagccatgg tgattcttgc ttttcttttt
     3001 acaataatag cggacatggg cgtaatagca tcggtcatca ctatactggg agtctgggtg
     3061 gccacggcca tgtcagctca gtgcgtggga cagtccggca taaatcccat ggaaatattc
     3121 gggataatcg ttcttatagt tgctaaagct gcttcagcag cgggatcact tgccggaata
     3181 ggcgagatag cagcattctt tgtggctgcc gttgtagcgg tggcctgtgg cctggtgggc
     3241 gacataatga acgatttcaa agccggatat atcttgaaat cggatccaaa agcccaatgg
     3301 tatgctgaat gcataggttc tttgacagga gcattcgttg cagttgtcgt actgctggcc
     3361 atagtaaagg catatggggg agaagtattc ggcagtgaag ttttccccgc tgctcaggcg
     3421 gctgccgtat ctgcaatggt aggcggaata tcccatatga cagcattcct tttgggcatg
     3481 gcaatcgcag tggtgcttta ctgttttgat tttccggtaa tgacactggg acttggtgtt
     3541 tatcttccct tctatttatc tgcgactgct tttgtaggag gagccttaag gttcatactt
     3601 gacagagcag caccggaatg gtcaaaaaga ggaatgggac aggtcatcgc cgcaggagtt
     3661 cttggaggag aagccatcac cggtgttgtg atcgccatca taatcgctgt ccggatggtg
     3721 ttatgataag aaagagaatg aaaccatgaa agagataaaa ataacagaca taaaaggcat
     3781 aaaagtcggc aacgcacagt ctatcagggg aggaaccgga tgtacggcaa tcatatgtga
     3841 agccggcgct tttgccggag tagatgtcag aggcggggct cccgcatccc gggagaccga
     3901 actgcttaaa ccggtaaata cggttaagca gatacactgt gttatgcttt cgggaggaag
     3961 tgccttcggt cttgcagcat gtgacggagc tatgcagtac ctggaagaaa agaaaatagg
     4021 ctttgatgtc ggcataggca tggtgcctat cgtatgcggg gcatctctgt ttgaccttgt
     4081 tttgggagat cccaaaacaa gacccgacaa agaaatggga tatgaggcat gtaaagccgc
     4141 tgaaaacgga gaacctgccc agggcaatga aggggccgga acaggagcat ctgtaggcaa
     4201 attcctcggc accgaaagaa tgatgaaaag cgggcttggc atatacgcag ttcagctggg
     4261 aaagataaag tgcggcgctg tagctgcagt caattctctt ggagacatag ttgattatga
     4321 cacaaaaaaa gtaatagccg gcattttgtc cgaagacaga aaaagccttt catgtacgaa
     4381 aaaaataatg tatcaggaaa ttgaaagacc caggaatgta tttacaggaa atacaactgt
     4441 aggatgcgtg gtgaccaatg cgaatcttga caaggatcag gcatgcaaac tggcatccat
     4501 agcccataac ggatatgcaa gaaccattga accggtccat acggcagctg atggagatac
     4561 tgtctttgtc atgtccacag gagaagagaa ggcggccttt gatcctttgt cggctctggt
     4621 ttcggaagtg acgggccgag ccgttaataa tgctgtaaag aatgcaaaac ctgcatacgg
     4681 ccttcccgct gcctgtgatt tcagatgact gtgaggatgg aagaagaaaa aaagtatcgg
     4741 gagaaaaaac attgaaaaaa ttaaaaatgt taaaagccat aatgaaaatg acccatgcag
     4801 acaaactgct gatatcatat gtggtctttc ttgtgatcgg atcatttctt atcatgctaa
     4861 cagatccgac tatcaatact ttcggaaatg ctttatggta ctgctatacc atgggagtta
     4921 caataggttt tggggatctg gtggttacga ctgtctttgc aaaagtgatt tctgtattga
     4981 tttcccttta tacgatcctt atagtaggaa tgataccggc tatagttgtc agttattatc
     5041 ttgaagtggt caagctcaga gaaaaagaaa cctccactca ttttttatat aagctggagc
     5101 atctttcgga gctttccaaa gaagaacttg aagagctttc ggagaaagta aaaaagttcc
     5161 aaaaaaaaca atgaaaaaat gaccggcttg ccggtcattt ttattaatag ggcgtttaag
     5221 cccgtgtcga gtgcgcgggc gcacgagata aa
//
LOCUS       DAONHK010000075         5249 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_12191_length_5249_cov_4.160762, whole genome shotgun sequence.
ACCESSION   DAONHK010000075 DAONHK010000000
VERSION     DAONHK010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5249
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12191_length_5249_cov_4.160762"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..146
                     /locus_tag="PLM92_03795"
     CDS             <1..146
                     /locus_tag="PLM92_03795"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006566635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HPR24296.1"
                     /translation="HDAPWHKNFGGSIYVRNGSHGCVNMPEYAAASVYANIYAGYPVV
                     VHY"
     gene            complement(148..807)
                     /gene="hypB"
                     /locus_tag="PLM92_03800"
     CDS             complement(148..807)
                     /gene="hypB"
                     /locus_tag="PLM92_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HPR24297.1"
                     /translation="MSNVKVIEIKKSVFENNDAEAEKLRRRLKEKGVFLLNLMSSPGS
                     GKTSVLARTIRELHDEMAIGIMEADIDSAVDAETIAATGVKTIQIHTGGMCHLDAGMT
                     EKGLLELGTEDIDLVVLENVGNLVCPAEFDTGASKNAMILSVPEGDDKPLKYPLMFST
                     VDLLLINKIDCLTYFDFDMNAVYERVRKINPGIEIIEVSAKTGEGFDKWTDWLRENIK
                     V"
     gene            998..1999
                     /gene="msrB"
                     /locus_tag="PLM92_03805"
     CDS             998..1999
                     /gene="msrB"
                     /locus_tag="PLM92_03805"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019119048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HPR24298.1"
                     /translation="MKKKTIVISACAFLFASILFVTGTEKEQKDPEDKKIMLNEGKII
                     YLAGGCFWGMEKLFQSLPGVTDAVSGYANGKKDILPDYRSVCTGQTGYRETVRVSYDP
                     KKISLEALLFAYYYVIDPRVKDGQGNDKGSQYQTGIYYDDEDSGETVIRISDIVKKRT
                     DKFYVEIEPLENFHEAEEYHQDYLDKNPGGYCHVNPAEINEIIKIIDVTQNSATERPF
                     DNLYWKNHEKGIYVDILTGQPLFASTDKYDSSCGWPSFSKPIDENALIEKKDDSLGMS
                     RTEVRSSQGDSHLGHVFTRDSESPTGTRYCINSASLRFIPYENMEKEGYGHLKASVR"
     gene            2059..2802
                     /locus_tag="PLM92_03810"
     CDS             2059..2802
                     /locus_tag="PLM92_03810"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020072302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HPR24299.1"
                     /translation="MDRLKGKVAIITGATSGMGRDTAYVFAEEGAKVMITGRNEERAR
                     EVVENIKKNGGEASYVIADIADRASLDKIVDDTVAKYGTIDILYNNAGVLSIQSSMDI
                     SLEEWDKVMQVNFTSALMLAKKVAPIMKAKGKGYIINTGSVAGTSARWGAAAYCTSKH
                     AMNGLTKALARELGPEIRVNAILPGAIVTAMLDSVGGEDAVAGMKEMSPLKRAGQGRE
                     IGTAALFFATEDSSFVTGQLLRVDGGVDC"
     gene            complement(2831..3454)
                     /locus_tag="PLM92_03815"
     CDS             complement(2831..3454)
                     /locus_tag="PLM92_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPR24300.1"
                     /translation="MSKNNISKETGMFIPLCAGLLSFFVSGVIACVVIGLTDNYIFAT
                     IITGGIGSFLLGFLIRSIRKNLFRTTIVGALSMPLALAVGFFLVEGAGALLPQMANKI
                     ESIGMDNIIAIILIASIFGTIMGIVIYGIYSIRVFLPICAIAGIPAGALVIALNGSWR
                     SSATYNAIMETFWQVDLNFLSMAIFMGIGMGVSVGIYNRIKQKRIHP"
     gene            complement(3787..4731)
                     /locus_tag="PLM92_03820"
     CDS             complement(3787..4731)
                     /locus_tag="PLM92_03820"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HPR24301.1"
                     /translation="MENIYKTSYNRFKLKWSKSPQATGYYIYRKSPGKGFKKIATIKK
                     VSGLTYTDKKFVCNTAYTYMIIAYYSNGSTLIKSSASNAFSKKSKYVGKQTVNKCIYY
                     FGKNYKCYRKVNGRKKMICLTYDDGPTSKTKSILATLKKYDQAATFFVVGSRVSKYKS
                     TIKRAYKQGCEIGNHTYSHPMLTLCSYSRVKSQMSRTKRAIKRITGSAPAVMRPPYGA
                     FNRTVRSAVGVPLIYWSIDTRDWATRSSRSTIKKVMNGARNGSIVLMHDTQSSSATAS
                     KTIIPRLVKKGYQLVTVSEMAELRSGMKKGKVYYKFYK"
     gene            complement(4788..5171)
                     /locus_tag="PLM92_03825"
     CDS             complement(4788..5171)
                     /locus_tag="PLM92_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPR24302.1"
                     /translation="MTKKLSKIAFAFIMSVALIMAMIPSNSVVFADEITDPQNEQEQT
                     VPSVPQISGVTCLSDGTVSVVWAAAENAESYQVYRSSSASGGFVPVAETVNTRYTDRY
                     VTVNKSIITKSAVSEGYRLKKARSY"
BASE COUNT         1517 a         1156 c         1055 g         1521 t
ORIGIN      
        1 tgcatgatgc tccctggcac aaaaacttcg gaggaagcat atatgtaaga aacggcagcc
       61 acgggtgtgt taatatgccc gagtatgccg ccgcttcggt ctatgcaaat atctatgcgg
      121 gatatcccgt tgtagtccac tactgatcta cacttttata ttttccctaa gccagtcagt
      181 ccacttgtca aagccttctc cggtttttgc ggagacttct attatttcta ttcccggatt
      241 gattttcctt actctttcat aaacggcatt catgtcaaaa tcaaaatacg taagacagtc
      301 tatcttgttt atcaaaagca gatccaccgt gctgaacata agaggatatt tcaaaggctt
      361 atcatcgcct tccggtacac ttaatatcat ggcatttttt gatgcccccg tatcaaattc
      421 cgccgggcac acaagattgc ccacattttc cagaactaca agatcaatgt cctccgtgcc
      481 aagctccaga agtccctttt ctgtcattcc cgcatccaga tgacacatac cgcccgtatg
      541 aatttgtatg gtctttacgc ctgtggctgc gatggtctcc gcatcaactg cgctgtctat
      601 atccgcttcc attattccga tcgccatctc atcatgaagc tctctgatgg tccttgccag
      661 aacactggtc tttcccgatc cgggggaaga catcaaattg agaaggaaaa cccccttctc
      721 ctttagtctc ctcctcagtt tttctgcttc tgcatcgttg ttctcaaaaa cacttttttt
      781 gatttctatt acttttacgt tgctcatttt ttctttctcc gctgattctt cctgttggtt
      841 tatttttata atattttgct ttgaatggat tttgataata tatattgtac cataaagtgc
      901 cggcaagtcc aatggatcaa agttttgtaa gggcattaaa aatatctgtt tcagttcacc
      961 ttccgatggg taaatgattt gcatataggt gttatatatg aaaaaaaaga caatagtgat
     1021 ttctgcctgt gcatttcttt ttgcctccat tttgtttgtc acaggcacag aaaaagaaca
     1081 aaaagatccg gaggataaaa aaatcatgtt aaacgaaggt aaaatcatat acctggccgg
     1141 aggctgtttc tggggaatgg aaaagctgtt tcagtccctt cccggtgtca ctgatgcagt
     1201 aagtggatat gccaacggga aaaaagacat ccttcccgac tacaggtccg tctgcacagg
     1261 tcaaaccggt taccgggaaa ctgttcgtgt ttcctatgat cccaaaaaaa tcagtctgga
     1321 ggctctgctt tttgcatact actatgttat agatccccgg gtaaaggacg gccagggcaa
     1381 tgacaaggga agtcagtacc agacaggtat atactatgat gatgaggatt ccggcgaaac
     1441 tgtaataagg ataagcgata tagtaaaaaa acgtacagat aagttctatg tggaaataga
     1501 gcccctggaa aatttccatg aagcggagga atatcaccag gactacctgg ataagaaccc
     1561 gggcggttat tgccatgtaa accctgcaga gataaatgag atcataaaga taatagatgt
     1621 gacacagaat tccgccaccg aaagaccctt tgacaacctt tactggaaaa atcatgaaaa
     1681 aggaatatat gtggatatac ttacgggaca acccctgttt gcctccaccg ataaatacga
     1741 cagttcctgc ggatggcctt ctttctcaaa acccatagat gaaaatgccc ttatagagaa
     1801 aaaagatgat tccctgggaa tgagcaggac agaggtaaga agcagccaag gagactccca
     1861 tctgggacat gtttttacca gagacagcga atcccctacc ggaacaagat actgcatcaa
     1921 cagcgcatct ttgagattta taccctatga aaacatggaa aaagaaggat acggtcactt
     1981 aaaagcatca gtcaggtaat aataaaaaat caaaaaagat aagttaaata ataattaaat
     2041 gaaaggtagg aacaaaaaat ggacagatta aaaggtaaag ttgccatcat cacaggagcc
     2101 acttcaggca tgggaagaga tacagcttat gtatttgccg aggaaggggc aaaagtaatg
     2161 atcacaggca ggaatgaaga aagagccagg gaagtcgttg aaaacatcaa aaagaacggc
     2221 ggagaggcat cttacgttat tgcagacatc gcagacagag catctcttga taagatcgtt
     2281 gatgataccg ttgcaaaata cggtactatc gatatcctct acaacaatgc gggtgttctc
     2341 agcatacaga gctcgatgga tatatctttg gaagaatggg acaaggtaat gcaggtaaac
     2401 ttcacttcag ctctgatgct tgcaaaaaaa gtggctccca taatgaaggc caaaggtaaa
     2461 ggatatatca taaacaccgg ctccgtagcc ggcacatctg ccagatgggg tgccgctgct
     2521 tactgcacat ccaaacatgc catgaacgga ctgacaaagg cccttgccag agaacttggc
     2581 ccggaaatac gggtaaatgc catactgccg ggagccatcg taactgccat gcttgacagc
     2641 gtaggcggag aggatgcagt tgcaggcatg aaagaaatgt ctcctcttaa gcgcgcaggt
     2701 cagggaagag aaatcggaac tgcagctttg tttttcgcca cggaagattc ttctttcgta
     2761 acgggacagc tgttgcgtgt tgacggcggt gtagactgct agccgaatcg tataaaaaaa
     2821 gggtccgccc tcaagggtgg atcctttttt gttttatcct gttgtaaata cccaccgata
     2881 ctcccatgcc tattcccatg aaaatcgcca ttgaaagaaa gtttagatct acctgccaga
     2941 aggtctccat tatagcatta taggtggcac tgctacgcca cgaaccgtta agggcgataa
     3001 ccagtgctcc ggcgggaatt ccggcaatgg cacatatcgg aaggaatacc ctgatagaat
     3061 aaatgccgta aatgacaata cccattatcg ttccgaatat ggaggctatc aggataatgg
     3121 caataatgtt atccataccg atgctttcta ttttatttgc catctgcggt agcagtgcac
     3181 ctgccccttc cacgagaaag aagcccactg ccagggccag cggcatgcta agagccccaa
     3241 ctattgtagt tctaaaaaga ttttttctta tgctccttat gagaaaaccc agcaggaatg
     3301 atccaatccc tcctgtgatt attgtggcga atatataatt atctgtcagt cctatcacaa
     3361 cacaggctat gactccgctc acaaaaaacg acaggagtcc ggcacatagc gggataaaca
     3421 tccctgtttc tttggatata ttattcttag acatttttat tccccctctc caacaaaaga
     3481 tctatcccca taaatccttt ctgtttacct atattataac atgaacaatc aagggttttt
     3541 atgattgtat gaaaatttta ctttttcccg gtttatcaat cattgaaagc ccatctcaca
     3601 aaattccgat ttacctgctt attattttca tataaatctc cgggagtgtg actaaaaaaa
     3661 ataaatacta tagctcttga tcaggagaga catgctctca acagaaaaag cagtacttac
     3721 ttttataata ttatataata caaggatccg tcatatgcct gtcgatttgt ttgatcctgt
     3781 gatcttttat ttgtagaact tataatatac tttacccttt ttcatgccac tgcggagctc
     3841 tgccatctca cttaccgtca ccagctgata gccttttttg acaagccttg gaattatggt
     3901 cttagatgca gttgcagaag aggattgagt atcatgcata agaactatgg aaccattacg
     3961 agccccgttc ataacttttt ttattgtcga acgggaactg cgtgttgccc agtcacgggt
     4021 atctattgac cagtaaataa gtggtactcc cacagccgac ctgacagtcc tgttaaaggc
     4081 cccgtaggga ggtctcatta ccgccggtgc cgaacccgtg atccttttta tagccctttt
     4141 agtcctgctc atctgacttt ttactctcga ataagaacac aatgtgagca ttggatggga
     4201 atatgtgtga ttccctattt cacacccctg tttataagcc ctttttatgg tggacttata
     4261 ttttgaaacc ctgctaccta ctacgaagaa agtggccgcc tgatcatact tcttcaaagt
     4321 ggccagaata gactttgtct ttgatgtcgg accgtcatca taagtgagac atatcatctt
     4381 ttttctgccg ttgaccttgc ggtaacattt ataatttttc ccaaagtaat atatgcattt
     4441 attcacagtc tgttttccca catacttgga ctttttagaa aaagcattac tggcagaact
     4501 ctttatcagt gtgctgccat ttgaatagta agcaattatc atataagtgt aagctgtatt
     4561 acatacaaat ttcttatctg tgtaagtcaa accggaaact ttcttgatcg ttgctatttt
     4621 tttgaatcct ttcccggggc ttttcctgta aatatagtaa cctgtagcct gtggcgattt
     4681 gctccattta agcttgaatc tattgtatga agttttataa atgttttcca atcttacata
     4741 ctgcagtttc accggcccgg aggctttggc agcacacagg tcgctcttta atatgatctc
     4801 gccttcttca gtctgtatcc ttctgatact gcagactttg taataatact tttatttact
     4861 gtaacatatc tgtcggtata cctggtattt acagtttcag ccacaggaac gaatccacct
     4921 gaagcactgc tgcttcggta gacctggtag ctttctgcgt tttctgcagc agcccatacg
     4981 acactgaccg ttccgtcaga aagacatgtc acaccggaaa tctgaggcac cgacggtact
     5041 gtctgctcct gttcgttctg agggtcagtg atctcatcag caaaaaccac agaattggat
     5101 ggtatcatgg ccataatcaa ggcaacgctc ataataaatg caaaagcaat tttcgaaagt
     5161 ttttttgtca tgatattgct cctcttatga tatatatacc attatatcat ttatatttca
     5221 atgtttttct cttgtgccta tatgaggtt
//
LOCUS       DAONHK010000076         5197 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_12354_length_5197_cov_4.577791, whole genome shotgun sequence.
ACCESSION   DAONHK010000076 DAONHK010000000
VERSION     DAONHK010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5197
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12354_length_5197_cov_4.577791"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(156..902)
                     /locus_tag="PLM92_03830"
     CDS             complement(156..902)
                     /locus_tag="PLM92_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014185548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPR24303.1"
                     /translation="MHKKTLKSKHGYDIPCVTNISGKEKKVIIISHGFGSSKASPTAQ
                     MIMNVIGPDETGTLAYDFPAHGESPADGKYLTLEGCLDDLCSVEHFVHKASPEADIMY
                     FSSSFGAYINLLYLSKRTPLGNRSFLRSAAVNMPELFLHPTTEEKEQLSSPGYLMLDR
                     GYEHPLMITKEFVDGLMKNDIFHSFTPSPGVSIAMVHGDMDECIPCSKAKEFAEKYGI
                     GLTVIKGGDHRLMTPGSPEKVLRLAVEFFG"
     gene            1043..1125
                     /locus_tag="PLM92_03835"
     tRNA            1043..1125
                     /locus_tag="PLM92_03835"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1077..1079,aa:Leu,seq:tag)
     gene            1504..2865
                     /locus_tag="PLM92_03840"
     CDS             1504..2865
                     /locus_tag="PLM92_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009253422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HPR24304.1"
                     /translation="MGKELIFRSLMHEKTERVPWVPFAGVHAGKLKGYTAEEMLKDPD
                     KIVTSLEEVAKIYMPDGMPVVFDLQIEAEILGCELLWAKDNPPSVKTHPLDSEKSIPC
                     TCKFPSVDSGRIPVVLEAMKRVKRSVGEDIALYGLICGPFTLASHLRGSSIFMDMVKD
                     PKYVKELIGYCGQVACRMADIYMEAGMDVIAVVDPLISQVSPKHIEKMMLETFTAVFD
                     YIRSRERFSCFFVCGNASKQIDVMCRTGPDGISVDENVDLPKAKEITDRYNINIGGNI
                     PLTTTMLHGTQQDNMKKVMDLMDSVSDHTNFIISPGCDMPYATPPENTVACAQAVKNP
                     ESTRELIKNYQPEGIIDIDIEIPDYEASEKVIIELFLLDPEQCAACTYMLAAVEDAYE
                     DIKDMAEYRVYKYFIKEDIARTAKMGVKNLPSICVNGEIKWISIIPSREELINVIKEY
                     ADK"
     gene            2862..3779
                     /locus_tag="PLM92_03845"
     CDS             2862..3779
                     /locus_tag="PLM92_03845"
                     /inference="COORDINATES: protein motif:HMM:NF014542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HPR24305.1"
                     /translation="MIKVFLITGFLGAGKTTFLKEAFDAFKGMKTGAVVNEFGDINID
                     GMVLRREGIKLAELTGGSIFCSCIKDKFVDSLIEMSKMDMDIMFIEASGLADPANMGE
                     IIAGIKHKTGDVYDYKGSLCIADGESFAELSDILIAVKTQVEFASAVIINKIDLIDDG
                     KLEKVKAKIREINPLIPIYGAVRCKLDIKKVIADMEPSGKPPEKSKNTPENRAETFVV
                     KGQRAVGMDALKGFIKDIAGESYRVKGFCITEEGPVRISASGTAIEFFPWKEEIGDTK
                     IVVISAIGFRMMSVILRSIEKNAKGIFTV"
     gene            3855..>5197
                     /locus_tag="PLM92_03850"
     CDS             3855..>5197
                     /locus_tag="PLM92_03850"
                     /inference="COORDINATES: protein motif:HMM:NF036965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPR24306.1"
                     /translation="MEIINKEISISKEKCMPLAEKLFAESSGMSKKRKSYDNQKKQSL
                     ETLDMIYDRIGIKAVFSYYEDICFEGKKAVIGGVDLVSNAFEQMDPFRIKGAYVYMLT
                     VGDSGLKDGPVIKQLLADIWGTCFVDAARMILEERFTSEGKRLSDSFGPGFYGMDIRS
                     MKEIKKLADGEKIGIGMSREGLLIPEKSCAGIYFSVTEDYAKLKDECGDCKGTEKSCA
                     FCNINKEKETRCTGECEICGRCTSGGVINDEDRTKAPVFVLPEDFSPVKKLPGYGIAF
                     DMGTTTVAGLLWDLENGREILSLARSNPQRAYGGDVISRISFIRKDPENLRVMRDAIR
                     DCLNGIITELCHGAGVKKNSLSKVTLCGNTTMSHIFSGHDPSSLAKAPFIPAYRGEVL
                     MNKETSGLDINEDAKVMLLPNIAGHVGGDTVAGMLSSRMTEQKELTLFIDIGTNG"
BASE COUNT         1619 a          956 c         1279 g         1343 t
ORIGIN      
        1 aagacaccgc cctttcacgg cggtaacccg ggttcgattc ccggtagggt caccagaaaa
       61 cccccaaaca gttggaagtt aaacagtttg ggggcgtttt tttgatacag attgaagtat
      121 ccaagaatat ttaattattt tttcgaatca aaaaattatc cgaaaaattc gactgcaagg
      181 cggaggactt tttcaggaga accgggtgtc atcagcctgt ggtctccgcc tttgatcacc
      241 gtaagaccta ttccgtactt ttctgcaaat tcttttgctt tgctgcaagg aatacattca
      301 tccatgtcac catggaccat ggcgatggaa accccgggtg acggcgtgaa tgaatgaaaa
      361 atatcatttt tcataagacc gtctacaaat tcttttgtga tcatcagggg atgttcataa
      421 cctctgtcca gcatcagata tcctggggaa gaaagctgtt ccttttcttc tgttgtggga
      481 tgaaggaaga gttcgggcat attgaccgca gcacttcgaa gaaatgatct attccccagg
      541 ggggtcctct ttgacagata aagaagattt atatatgcgc cgaagctgga ggaaaagtac
      601 atgatatcag cttccggaga tgctttgtga acgaaatgtt ctacagaaca aagatcatca
      661 aggcagcctt ccaaagtaag atattttccg tctgcagggc tttcgccgtg tgcaggaaaa
      721 tcatacgcaa gggtgcccgt ttcatcggga ccgatgacat tcatgatcat ttgtgcagta
      781 gggcttgcct tgctgctacc gaagccgtga cttattatta tgactttttt ttcttttccc
      841 gagatgttcg tgacacaggg gatgtcatat ccgtgtttgc tttttagtgt ttttttgtgc
      901 atttttttct ccctgatgac cattttacat cagtttgctc tgaaaacaaa gagtgtcttc
      961 gatttttttt actaatctgt tgaagtcaga ggaaaaataa tgtatattat tttaatggcc
     1021 gttaaagacg gcatgatcat atgcgcccat ggcggaattg gcagacgcgc tagatttagg
     1081 atctagtggg agaccgtagg ggttcaagtc cctttgggcg caccaaagaa aaatgagatg
     1141 gggttatccc atctcatttc cgtatgtata gatgaaagga gggacttgaa cccgaaaggg
     1201 cgtgaaggtc cggtggacct tcacggtccg agcacgctga agcgagcgca ggacggagga
     1261 ttgccgaggg caatacgcgt aagtcccttt gggcgcacca ttttatccga aagcttattt
     1321 ttccataaag gtacagtatg cctcctgaat cgatttttta ataaataccg cttcccggga
     1381 ggtttttatt tgtcacttct ggggatgcag gttataatgt ttcagagaca ttgttattga
     1441 gcgatatgtt ttttgacaaa aaatatattt aatttgatat atctttgtaa cagggaggac
     1501 aaaatgggaa aagaacttat tttcagatca ttaatgcatg aaaaaacaga aagagtgcca
     1561 tgggtgcctt tcgcaggagt tcatgccgga aaactgaagg gatatacagc ggaggagatg
     1621 cttaaggatc ccgacaagat cgtcacatct ctggaggaag tcgcaaagat atacatgcct
     1681 gacgggatgc ctgtggtctt tgatctgcaa atagaggcgg aaatcctcgg atgtgaactt
     1741 ttgtgggcaa aggacaaccc cccttcagtc aaaactcacc ctcttgacag tgaaaaaagc
     1801 ataccatgca catgtaaatt cccttctgtc gattcgggca ggataccggt cgttctggaa
     1861 gccatgaaaa gagtcaaaag atccgtggga gaagacatag ctctttatgg cctgatatgt
     1921 ggtcccttta ctctggcttc gcaccttaga ggaagcagta ttttcatgga tatggtcaag
     1981 gatccgaaat acgtgaaaga gcttatagga tactgcgggc aggtggcatg ccggatggcg
     2041 gatatctaca tggaagcggg aatggacgtt atagcagtgg tggatcctct tataagtcag
     2101 gtctcgccaa aacatataga aaaaatgatg ctggaaactt ttaccgcggt atttgattat
     2161 attagaagca gagaaagatt ttcatgtttc tttgtttgcg gcaatgcctc aaaacagata
     2221 gatgttatgt gcaggacagg tcctgacgga atatcggtcg atgagaatgt ggatctgcca
     2281 aaggccaagg aaataacaga cagatacaat ataaacatag gagggaacat tccccttacg
     2341 accacaatgc ttcacgggac ccagcaagat aacatgaaaa aggtcatgga cctcatggac
     2401 agtgtcagtg atcacaccaa ttttataatc agtcccggat gtgacatgcc ctatgcaaca
     2461 cctccggaaa acactgtggc ctgtgctcaa gccgtaaaaa accccgagag caccagagaa
     2521 ctgataaaaa actatcagcc ggaggggatc atcgatattg atattgaaat accggattat
     2581 gaggcatccg agaaagtcat aatcgaattg ttcctgctgg acccggagca gtgtgcggcc
     2641 tgcacataca tgctggctgc agtagaagat gcatatgaag atatcaagga tatggcagaa
     2701 taccgggtat acaagtactt cataaaagaa gatattgcaa ggacggcaaa aatgggcgtc
     2761 aaaaatcttc ccagtatatg tgtcaacggg gagatcaaat ggatatccat cattccgtcc
     2821 agggaagaat tgataaatgt aataaaggaa tatgccgaca aatgataaaa gtttttctta
     2881 taacaggatt tctgggagcc ggtaaaacca catttctgaa agaagcattt gatgcattca
     2941 aaggaatgaa aacaggggct gtggtgaatg aattcggtga cataaatata gacggcatgg
     3001 ttttaaggcg tgaaggaatt aagctggcgg aactgaccgg cggttcaata ttctgttcat
     3061 gtataaaaga taaattcgtg gacagcctta ttgaaatgtc gaaaatggat atggatatta
     3121 tgtttattga ggcctcaggg ctggcagacc ctgcaaacat gggagagatc atcgcaggca
     3181 taaagcataa aacaggggat gtatatgatt acaaaggttc tctgtgcata gcagacggag
     3241 agagttttgc ggagctttcc gatatactga ttgctgttaa aacgcaggtt gaatttgctt
     3301 ctgcagttat aataaacaag atcgatctta tcgacgatgg aaaactggaa aaggtaaagg
     3361 ccaaaataag agaaatcaat ccgctgatac ccatatacgg agcggttcgg tgtaaactgg
     3421 atataaaaaa ggtcattgcc gatatggagc cttccgggaa accacccgaa aaaagcaaaa
     3481 atactcctga aaacagagcc gaaacatttg ttgtaaaagg tcaaagggca gttggcatgg
     3541 atgcgctgaa aggtttcata aaagatatag ccggggaaag ctatagagtg aagggatttt
     3601 gcattactga agaagggcct gtccggataa gtgcttcggg taccgccatc gagttttttc
     3661 catggaaaga agagattggc gacacgaaaa tagtggtcat atctgcaata ggattcagga
     3721 tgatgagtgt tattttgaga tccatcgaaa aaaacgcaaa gggcattttt acagtttaga
     3781 agatgccaca gaatatttgt gaatatctgt gcatctccga aaactgcaaa accactaaaa
     3841 gaacagagga gcaaatggaa ataataaaca aagagatctc catatccaaa gaaaaatgta
     3901 tgccgctggc agaaaaactt tttgccgaat caagcggcat gtccaaaaaa agaaagagtt
     3961 atgataacca aaaaaaacag tctctggaaa ctcttgacat gatatatgac aggatcggga
     4021 taaaggctgt cttttcttat tatgaagata tttgttttga ggggaaaaaa gctgttatcg
     4081 gcggagtgga tcttgtttcc aacgcctttg agcagatgga tcctttccgg ataaaaggag
     4141 catacgtata tatgcttact gtcggcgact ccggacttaa agacggtccc gtaataaaac
     4201 agcttctggc ggatatctgg ggaacatgtt tcgttgatgc ggcaaggatg atcctggaag
     4261 aaagatttac ttccgaaggt aaaagactga gtgattcttt cgggcccggc ttttacggta
     4321 tggatatcag aagtatgaag gaaatcaaaa aacttgcaga cggtgaaaaa ataggtatcg
     4381 gcatgagcag ggaaggtttg cttatacccg aaaaatcgtg tgcagggata tatttctcag
     4441 taactgaaga ctatgcaaaa cttaaggatg aatgcggaga ctgtaaaggg actgaaaaaa
     4501 gctgtgcctt ttgtaatata aataaagaaa aggaaacaag atgtaccggt gaatgtgaaa
     4561 tatgcggaag atgcacaagt ggtggagtca taaatgacga agacagaact aaagcacctg
     4621 tatttgttct gccggaggat ttttcgcctg taaaaaaatt gcccggatat ggaatagcct
     4681 ttgatatggg caccaccacc gtagcaggac ttctatggga tcttgaaaac ggccgtgaga
     4741 ttttgtctct ggccagatca aatccacaga gggcttacgg gggagacgtg atatcaagaa
     4801 tatcattcat aagaaaagat cctgaaaatc tgagagtaat gagagatgca ataagagact
     4861 gcctgaatgg gataataacc gaactgtgtc atggagccgg tgtaaagaaa aattctttat
     4921 caaaggtcac gttgtgcggc aatacgacca tgtcccatat tttttcgggg catgatcctt
     4981 cttctcttgc caaagcacct tttattcccg cttatagagg tgaggtttta atgaacaaag
     5041 agacatccgg actggatata aatgaggatg caaaagttat gcttttgccc aatatagcag
     5101 gacatgtggg aggagacacg gttgccggga tgctttcatc taggatgacc gagcagaaag
     5161 aactcacact gttcatcgat atcggtacta acgggga
//
LOCUS       DAONHK010000077         5167 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_12456_length_5167_cov_4.061033, whole genome shotgun sequence.
ACCESSION   DAONHK010000077 DAONHK010000000
VERSION     DAONHK010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5167)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5167)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5167
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12456_length_5167_cov_4.061033"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="PLM92_03855"
     CDS             complement(<1..273)
                     /locus_tag="PLM92_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SufBD protein"
                     /protein_id="HPR24307.1"
                     /translation="MDKDEIKKHVELLKSKDSIKGYEALKLLVAASNGSDDVHSYMEE
                     FFSMIDDSNSYIRTRALILIVENAKWDKEGRIEEEIERLLIHITDEK"
     gene            complement(332..1765)
                     /locus_tag="PLM92_03860"
     CDS             complement(332..1765)
                     /locus_tag="PLM92_03860"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016519121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HPR24308.1"
                     /translation="MKMIIDGKKADSIKGERFDIIAPATGKVIDSVPKATAEDIDISI
                     TAAVKAQKEWKNVPIYKRADILYKFLDIVEENKEDLAKTLSEENGKPIREARIEIRNI
                     KTAFSGFIESAKHYYGKIISPGAEAGYKKHMQLVTREPLGVVVCITPFNFPCDLYDHK
                     VAPALMMGNAAIALPSSDSALTLLKLTEMIVEAGVPGGVIQCLTAPGSVKSAAVSDRR
                     VSLVTLTGSTEVGIHTAAATAANLTGTALELGGNDAFIVLDDGDVDLAIKEMVLGRMY
                     NTGQVCCASKRFIIHNSIKEEFAEKAADRVKRLKIGDPLEEDTEIGCLINEKAAVKVE
                     KQVELTVKQGGKILTGGKRNGAFFEPTVITDLKRNADVMRDMEIFGPVVPIVGFDTDE
                     EALEIANDSKYGLSGCVFSENQKRAFKVAGAMEAGTAVINGSGFFRPFEMPFGGWKQS
                     GIGTEGVMTTFDKMTRIKTVVLKNIID"
     gene            complement(2209..2604)
                     /gene="rpsI"
                     /locus_tag="PLM92_03865"
     CDS             complement(2209..2604)
                     /gene="rpsI"
                     /locus_tag="PLM92_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007554258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HPR24309.1"
                     /translation="MAKMQYNGTGRRKSSVARVRLIPGKGNITINNKPLDDYLAMEIV
                     KREVKRPFEIAGCEGKFDVIARVHGGGTTGQAGALRHGISRALCQADRDAYRAVLKDA
                     GFLTRDPRMKERKKYGLKKARRASQFSKR"
     gene            complement(2623..3051)
                     /gene="rplM"
                     /locus_tag="PLM92_03870"
     CDS             complement(2623..3051)
                     /gene="rplM"
                     /locus_tag="PLM92_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005998792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HPR24310.1"
                     /translation="MKSFIAKPHEVERKWYIIDAEGRTLGRMATEAASILRGKKKTTY
                     TPHVDCGDYVIIINAEKVEVTGNKRKDKIYKHHSGYPGGLKEVTFEKMQEKHPEEIIR
                     HAVKGMMPNGKLGRKMFKKLKVYAGPEHDHSAQKPEPWEF"
     gene            3805..>5167
                     /locus_tag="PLM92_03875"
     CDS             3805..>5167
                     /locus_tag="PLM92_03875"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing phosphodiesterase"
                     /protein_id="HPR24311.1"
                     /translation="MINRKVIQKKQFVAIFMFSVFFFVALSIQYYNPSLLLSGMAACL
                     AVVMVYLTLQNPADMLDDLTGCLNRTALLRFIKERMKKKACCNAIIVSLDDLPEINKL
                     FGMDTGNEVLFNFAKFLKGISKKAFVFRLMGDTFIIATDKQKECFEIKDIISGKLKTS
                     SFTEDINIKISATLCYTRNVRCPAQESDVMLILLEGLQAAKSKGKGSVFEISQAFADS
                     VIFSFDMRSILKETLEKQDLQIWLQPIFSPATGKIIGAEALSRILHPEKGIISPADFI
                     PLAEKTGMITEISRQVLEKICSFIKEHEPFRYEGFECISMNLSVADIMQNDFPVKVND
                     CIEKHDLVPGSVIFEITETAAALGSETLKNNMKKLCEKNHEFAIDDFGTGYSNLSTLT
                     NLPFLILKTDRSVMPDDLSDKRQTSVFMKLIDLFKGVGLDVIVEGVETKEQHDLLLRL
                     GVKY"
BASE COUNT         1228 a         1195 c          980 g         1764 t
ORIGIN      
        1 cttttcgtcc gtaatgtgga tcagcagtct ttctatttct tcttctattc tgccttcttt
       61 gtcccatttg gcgttttcga caataagtat cagcgctctg gtgcgtatat atgaattgct
      121 gtcatcgatc atggagaaaa attcttccat ataagagtgg acatcgtctg atccattact
      181 ggccgccaca agaagtttaa gcgcctcata cccctttatt gagtctttgc tttttagcag
      241 ctccacatgt tttttgattt catccttatc cattttcttt tcctcttttt tttgaaaagt
      301 gtcttctttc tctgtcgaaa acttcaattg tctagtctat tatgtttttc agcaccactg
      361 tttttattct tgtcatttta tcaaacgtcg tcatcacgcc ttcagtacct atgccgcttt
      421 gcttccatcc tccgaagggc atttcaaagg gtctgaaaaa tccggatccg tttattaccg
      481 ctgttcctgc ttccatggca cctgccactt tgaaagccct cttctggttc tcggaaaata
      541 cgcagccgga aagtccgtac tttgaatcgt ttgctatctc caaagcctct tcatcggtat
      601 cgaatccgac tatgggcacc accggtccga aaatctccat gtctctcata acatcagcgt
      661 tccttttcaa gtctgttata acagtcggct caaagaaagc gccgtttctt ttgcctccgg
      721 tcaggatctt tcctccctgc tttactgtaa gttccacctg cttctcaact tttacggcgg
      781 ctttttcatt tatgaggcat cctatttccg tatcctcttc taaagggtct cctattttaa
      841 gtctcttgac tctgtctgcg gctttttccg caaattcctc ttttatcgaa ttatgtataa
      901 taaatctttt gctggcgcag catacctgtc ctgtattata catccttccc aacaccattt
      961 ccttgatggc cagatcaacg tctccgtcat ccagcacgat gaaagcatca tttcctccca
     1021 gctccagagc cgtcccagta agattcgctg ccgttgccgc tgccgtatgg atgccaactt
     1081 ctgtgcttcc cgtaagggtg accagactga ctcttctatc tgataccgct gcacttttta
     1141 cactacccgg agccgtcaga cattggatca cgccgccggg cactccggct tcaactatca
     1201 tttctgtaag tttgagcaaa gtaagggcac tgtcagatga gggcagtgct atggcggcat
     1261 ttcccatcat gagtgcagga gccactttat gatcataaag atcgcacgga aagttgaaag
     1321 gggttatgca taccaccaca ccaagaggtt ctctggttac cagctgcata tgttttttat
     1381 acccggcttc tgctcccgga gaaatgattt ttccgtaata atgctttgcg ctttcgataa
     1441 aacctgaaaa tgccgttttg atatttctga tctcaatacg ggcttctctg atcggctttc
     1501 cgttttcttc agaaagcgtc tttgcaagat cctctttgtt ttcttctact atatcaagga
     1561 acttatatag tatatctgcc cgtttatata tcgggacatt cttccattct ttttgagctt
     1621 ttacagctgc cgtgatgctg atatctatgt cttcagccgt tgcttttgga acgctgtcta
     1681 tcaccttacc tgtggcaggc gcaatgatat caaatctctc tccttttata ctgtcggctt
     1741 ttttgccgtc tattatcatt ttcatggtat taatgccccc ttgtttttgt ttcttaataa
     1801 aactataaac tgttcttctt taataagtca attgttattg gtaaatatgt gtttttttgg
     1861 tggtatttat ttcttattat atgcgtcgat ttaggagtgt tttgatttgt agaagatttg
     1921 tagaagattt gtaggcaaat aaaaacccac agggaactgc aggtgtatag atttcttgat
     1981 tttatgggtt tctttaccgt agagccccca taacaagctc ctgcactcct ggcggaggat
     2041 cttccttcga tttgcttcag ccacaataaa accccggaaa aattccgggg tttttgtgct
     2101 ttcgcatttc taataaccaa ttgcgccgta gagtgacacg gagcaagctc ctgcactcct
     2161 gtcggaggtc cttccttcga tttgcttcgc aaatcgggat aggtctctct atctctttga
     2221 gaactggctt gctcttcttg cttttttgag tccgtatttc tttctttcct tcatcctggg
     2281 gtctcttgtc aggaatccgg catctttcag gactgctctg taagcatctc tgtctgcctg
     2341 gcacagggct cttgatatgc cgtgcctcag ggctccggcc tgtcctgttg ttccgcctcc
     2401 gtgtactctg gcgataacgt cgaatttgcc ttcgcatcct gcgatctcaa agggtctttt
     2461 tacttctctc tttacgatct ccattgccag gtaatcgtca agaggtttat tgtttatggt
     2521 tatatttcct ttgccaggga tcaaccttac tctggctacg gaagattttc ttctacctgt
     2581 tccgttatac tgcatctttg ccatttcgtt tcctcccttt ctttaaaatt cccaaggttc
     2641 cggtttctgt gccgagtgat cgtgctcagg acctgcataa acctttaact ttttgaacat
     2701 ttttcttccc agtttgccgt ttggcatcat gccttttact gcatgtctta tgatctcttc
     2761 aggatgtttc tcctgcattt tttcaaaagt gacttcctta agaccgcccg gataaccgga
     2821 gtggtgctta tagatcttgt cttttctttt attgcctgtg acttccactt tctctgcatt
     2881 gattattatc acataatctc cgcagtccac atggggtgtg taagtggtct ttttcttgcc
     2941 cctcaatatg gaagctgctt ctgtggccat cctgccaaga gttctgccct cggcgtctat
     3001 gatgtaccac ttgcgctcaa cttcatgggg ttttgcgata aatgatttca ttttttcctc
     3061 cctgcctact ggtttccgca taaagaaagc ttttcggcac tgcctactgg gatcgctgat
     3121 ctttttcggc ataactaaaa cttgttcagc cgtgctgtct gccgggaaat ctttccctga
     3181 cgcacgaatg ccacgattta tggcactatg acagtttata tgttttttgt tgaaatgtca
     3241 aagattttat agaatatttt tttgttatat gatatatttg aatgagttag aaatttaaca
     3301 cgttaaagag aggtggctgt catactatac gatatcagct atgaaatctg cgctctgata
     3361 ttcctgtcag ttgtcgccgt gcattttttc agtgagtacc gcttcccgag tatcacaaat
     3421 cttattttca aaatcatatt aggggcaaca tttttttata cgctccttga tattgccacc
     3481 gcatatacca tttcttataa tgaccggttt tcatttttgc tgaatctaat gcttaatcag
     3541 ttgtttttta tgctccttat atttctggct ccccttatat atctttttat cctgagcctt
     3601 acagataagc ttacaaagaa gaaccgtgtt tttatcggca tatctttttt accctggctg
     3661 gcgttgttta tcatatgggt tctgaatccc ataagaggcg gcttttttac tttcgatata
     3721 tataatgatt acattcacgg acctttttat tacttgattt ttattatttc ggctttctat
     3781 ttatgccttg ccctttgcat gactgtgata aacagaaagg tcatacaaaa aaagcagttc
     3841 gttgccatct ttatgttctc tgttttcttt tttgttgctt tgtccataca gtactacaat
     3901 ccgtctcttc tgctttcggg catggccgca tgcctggcag tggttatggt atatctcact
     3961 ctgcaaaatc ctgctgatat gcttgatgat cttacgggct gtcttaaccg tacggctctt
     4021 ctgcgattca taaaagaaag aatgaaaaag aaggcctgct gcaatgctat aatcgtatcc
     4081 cttgacgatc tgccggaaat aaacaaactt ttcggaatgg ataccggaaa tgaggtactt
     4141 ttcaattttg caaaattcct gaaaggcatt tccaaaaaag catttgtctt ccgcctgatg
     4201 ggcgacacct ttataatagc caccgataaa caaaaggaat gttttgaaat aaaggacatc
     4261 atatccggga aactcaaaac ttcctctttt acagaagata taaatataaa aatatcggcc
     4321 actttatgct ataccaggaa tgtgagatgt ccggcccagg aatcagacgt catgctaatc
     4381 ctccttgaag gtctgcaggc tgcaaaatcc aagggaaaag gttcggtgtt tgaaatatct
     4441 caggcttttg cagattcggt gatcttcagt tttgatatgc gctccatatt aaaagaaact
     4501 ctggaaaagc aggatcttca aatatggctc cagcccattt tttctcctgc aacaggaaaa
     4561 ataataggag cggaagcatt aagcaggata cttcatcccg aaaagggtat cataagtccc
     4621 gcggatttca ttcctcttgc agaaaaaacc ggcatgatca cagaaataag ccgccaggtc
     4681 cttgaaaaaa tatgcagctt cataaaagaa cacgagccct tcagatatga aggttttgaa
     4741 tgtatatcaa tgaacctttc tgtagcagac atcatgcaga atgattttcc ggtaaaggtg
     4801 aatgactgta tagaaaaaca tgatctggtc ccaggctctg ttatattcga aataacagaa
     4861 accgccgctg ctttgggcag tgagactctc aaaaacaata tgaaaaagct gtgtgaaaaa
     4921 aaccatgaat tcgccattga tgacttcggc accggatact ccaatctttc aactcttaca
     4981 aaccttcctt tcctgatact gaaaacagac cgttcagtga tgcccgatga tctgtcagac
     5041 aaaagacaga cttccgtatt catgaaactg atagatcttt tcaaaggtgt cggtcttgat
     5101 gttatcgtcg aaggcgtcga aacaaaagag cagcatgatc tgctgctccg tcttggtgta
     5161 aaatatg
//
LOCUS       DAONHK010000078         5143 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_12544_length_5143_cov_4.385024, whole genome shotgun sequence.
ACCESSION   DAONHK010000078 DAONHK010000000
VERSION     DAONHK010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5143
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12544_length_5143_cov_4.385024"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1198)
                     /locus_tag="PLM92_03880"
     CDS             complement(<1..1198)
                     /locus_tag="PLM92_03880"
                     /inference="COORDINATES: protein motif:HMM:NF037180.2"
                     /note="catalyzes the formation of
                     2-(formamido)-N1-(5-phospho-D-ribosyl)acetamidine from
                     N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide and
                     L-glutamine in purine biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HPR24312.1"
                     /translation="MVRRIYVEKKPGFDAEAKNLMEDLKQDLHIKGLSDLRLINRYDI
                     QGIDKNTYEMAASSVFAEPALDDHWHEKLPAGKDAKVFASEYLPGQYDQRADSAAQCI
                     RIIKPEAKPIVKFARVYALYGDITEEELDKVIEYCINPVDSGLADDKKPLSLEMETVI
                     PDDVPRVKGFVKMDETRLEELRKEMGFAMSKADLMHIHDYFSKEEKRDPSLTELLVID
                     TYWSDHCRHTTFNTGLSNIYFEESPYGELVRNAFQEYMEMRNSVYGDRKKDVCLMDMA
                     CIGAKYLRKQGKADDIDESEEVNACSIKVRATVDGQEEDWLVMFKNETHNHPTEIEPF
                     GGAATCLGGAIRDPLSGRVYVYQAMRVTGAADPGTPVSETIKGKLPQRKITRQAAEGY
                     SSYGNQI"
     gene            complement(1369..3285)
                     /gene="htpG"
                     /locus_tag="PLM92_03885"
     CDS             complement(1369..3285)
                     /gene="htpG"
                     /locus_tag="PLM92_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015559187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HPR24313.1"
                     /translation="MAKKQFKAESKRLLDLMINSIYTHKEIFLRELISNGSDAIDKLY
                     YKMLTEEGSSGINRDNFEIEIKTNKTDRILKVSDNGIGMTKEEMESNLGVIARSGSLD
                     FREDAKDEEEDKKPKDQSDIDIIGQFGVGFYSAFMVSDQVTVISRAYGSDKAYKWESA
                     GTDGYTIEEHDKKDVGTEITLHIKKNEKKKGSGDEAADAEDYDSYLETYKIKQLVGRY
                     SDYIRYPIKMKEGGEIKTLNSMVPIWKQQKSKIKKEDYHIFYKQKFHDYVDPLKVVHS
                     SVEGVTSYSALLFVPSHAPYNYYTKDYEKGLQLYSSGVMIMDKCADLLPDYFNFIKGV
                     VDSQDLSLNISREMLQHDRQLKAIGGRLEKKIKTELVAMQKYDRENYEKFFKAFGLQL
                     KYGIYENFGMNKEALQDLVMFWSSTEKKPVTFAEYVARMPEEQKYIYYASGEDAEKID
                     RMPQTELLKDKGYEIFYLTEDVDEFVIQIMQSYEGKEFKSVSAEDLELEQTEEDKKAA
                     EEKEKENKELLEFMKESLEGKVSEVKLSQRLKSHPVCITAKGGLSLEMEKVLNQMPMA
                     DGNGMKAERVLEINSDHKIMKVLSKAFEAGDEKGKEKVREYTKLLYDQALLIEGMSIE
                     DPFAFVGSVCELMK"
     gene            complement(3565..4929)
                     /locus_tag="PLM92_03890"
     CDS             complement(3565..4929)
                     /locus_tag="PLM92_03890"
                     /inference="COORDINATES: protein motif:HMM:NF021196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPR24314.1"
                     /translation="MKRILFTCVGASDPVRGDFDGPMLHIIRHYRPETVYFYMSKEMS
                     KRKDEILLAINTVTGNIGHEIESIPLGSDKVDVSDFDAFNHEFEGYFKDIKEKHSDAE
                     LLVNISSGTPQMLITLALLSQDISFNIKAIQVSSPAKASNTSKTTTSKDYDFQEALDN
                     NFDDLEESENRCVEPKFIELKRSLAKEQLRALLYDYNYDGCEKFALAQGFTKDSRVIK
                     LISHLSERQNLKNSVEIQKEIIDYDFYPIKDSECKRVTEYMLILMQLQKMGKVTELLI
                     RLNPLVIRLQISFLENVFNIKKDSIASKKKDRYIFDTKKIENNYKELGERLNKKYRDG
                     FRDSDISVNVMKEIINFFANNKKYAGNLKKLNHIDYFEMFNRFDDVNEYRNKVAHSLI
                     AITEEELKKATNTGTADIIRNIKKIITFIHKNTYQEKILDVYDDVNEMILENINISAT
                     QELP"
     gene            complement(4955..>5143)
                     /gene="cas2"
                     /locus_tag="PLM92_03895"
     CDS             complement(4955..>5143)
                     /gene="cas2"
                     /locus_tag="PLM92_03895"
                     /inference="COORDINATES: protein motif:HMM:TIGR01573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HPR24315.1"
                     /translation="YGYRVQKSAFEAFLDSSKYKKMIKKIPRIIKSSDNVRVYMLEGE
                     GKYWGENRRDIKEDIIIL"
BASE COUNT         1173 a         1181 c          908 g         1881 t
ORIGIN      
        1 ctatctgatt gccgtatgag gaatatcctt ccgccgcctg gcgggttatc ttcctctgag
       61 gaagcttacc ttttatggtc tcactgaccg gagtcccggg atctgctgct cccgtaacgc
      121 gcatggcctg gtagacatat actctgccgc ttaatggatc tcttatggct cctccaagac
      181 atgtggccgc accgccgaag ggctcgattt ccgtgggatg gttatgggtc tcgtttttga
      241 acatcacaag ccagtcttct tcctgaccgt caacagtggc cctgaccttt atggaacagg
      301 cattcacttc ttcggactca tcaatgtcat ccgcctttcc ctgtttcctg agatattttg
      361 ctcctatgca tgccatgtcc ataaggcaca catccttttt cctgtcgccg tatacggagt
      421 tgcgcatttc catatactcc tgaaaagcat ttcttaccag ctctccgtag ggactctctt
      481 caaaatatat gtttgaaaga ccggtgttga aagtcgtatg cctgcagtga tcggaccagt
      541 atgtgtctat gaccagaagc tcagtcaggg atggatctct tttttcttct ttggaaaaat
      601 agtcatgtat atgcataaga tccgccttgc tcatggcaaa tcccatttct tttcggagtt
      661 cttcaagacg ggtctcatcc attttgacaa atccctttac cctgggcacg tcatcgggta
      721 tgactgtttc catttcaagg gaaaggggct ttttgtcatc cgccagcccg gagtcaacag
      781 ggtttatgca gtattcaatg accttgtcaa gctcctcttc tgttatatct ccgtaaagcg
      841 catagactct ggcgaacttt acgatgggtt tcgcttcagg ttttattatc cttatgcact
      901 gggcggcaga atcggccctc tggtcgtact gtccggggag gtattccgat gcaaacacct
      961 ttgcatcctt tcccgcagga agtttctcat gccagtgatc atccagcgca ggctcggcaa
     1021 aaaccgatga agcggccatt tcgtatgtat ttttgtctat gccctgaatg tcatatctgt
     1081 taataagccg cagatcggaa agccctttga tatggagatc ctgtttcaga tcttccatga
     1141 ggttttttgc ttccgcatcg aacccgggtt ttttctccac atagatcctt cttaccattt
     1201 atcttccttt ctgtataaat gttcggcttt tgatgggttt ttctccagta tttgctctat
     1261 attaacataa aaacagcccg ttttgggctg tttaagaaag ttttttaaat tatagttcgt
     1321 gtatattcga acattcttct gatctttaat ggtctttgtc tgtatttgct acttcatgag
     1381 ctcacataca gaacctacaa aagcaaatgg gtcctcaata ctcattccct ctataagaag
     1441 cgcctgatca taaaggagtt ttgtatattc cctgaccttt tctttgcctt tttcgtctcc
     1501 tgcttcaaat gccttggaaa gtaccttcat tatcttgtgg tccgagttta tctccaaaac
     1561 cctttcggct ttcattccat taccgtctgc catgggcatc tggttgagta ccttttccat
     1621 ttccagggaa agtcctccct tggcggtaat acataccgga tggcttttga gtctctgaga
     1681 aagtttcact tccgaaacct ttccttccag gctttccttc atgaactcaa gaagttcttt
     1741 gttctctttt tctttttctt ccgccgcttt cttatcttct tcagtctgct ccagctcaag
     1801 atcctctgcg ctgaccgatt tgaattcttt gccttcatag ctctgcatga tctgtatgac
     1861 aaattcgtcg acatcctctg tcagataaaa gatctcatat cccttatcct tcagaagttc
     1921 cgtctggggc atcctgtcta tcttttccgc atcttctccc gaagcataat agatatattt
     1981 ctgctcttcg ggcatccttg ccacatactc ggcgaaggtc accggtttct tttctgtgga
     2041 ggaccagaac atgaccagat cctgcaatgc ttctttgttc atgccgaagt tctcatatat
     2101 accgtattta agctgcagac cgaaggcttt gaaaaacttt tcgtaatttt ctctgtcgta
     2161 cttctgcatg gcaacaagct ctgtttttat ctttttttcc agtctccctc cgatggcttt
     2221 cagctgtctg tcatgctgca gcatctctct ggagatattc agagaaagat cctgggagtc
     2281 aaccacacct ttaatgaagt tgaaatagtc gggcagcagg tcggcgcatt tgtccattat
     2341 cattactccc gaagaataaa gctgcagacc tttttcgtaa tcctttgtgt agtagttgta
     2401 gggtgcatga gacggcacaa aaagcagtgc gctgtagctt gtcacccctt ccacgctgga
     2461 atggacgacc ttaagcggat ccacataatc gtggaatttc tgtttgtaaa aaatgtgata
     2521 atcttctttc ttgatcttgc ttttctgctg cttccatatg ggcaccatgc tgttcagtgt
     2581 ctttatctct ccgccttcct tcattttgat cggatagcgg atgtaatcgg aatatctgcc
     2641 caccagctgt tttatcttgt aggtctcaag atagctgtca tagtcttctg catctgcagc
     2701 ttcatcgccg gatccttttt tcttttcatt ctttttgata tggagagtta tctcagtgcc
     2761 cacgtctttt ttgtcatgct cttcaatggt atagccgtct gttccggcgg attcccattt
     2821 gtatgcttta tcgctgccat aggccctgct tattacagtt acctgatcgc tgaccataaa
     2881 tgcggaataa aaacccactc cgaactgtcc gataatatcg atatccgact gatctttcgg
     2941 ttttttatct tcctcttcgt cctttgcgtc ttctctgaaa tcaagagaac cgcttctggc
     3001 gatgactccc agattggatt ccatttcttc tttggtcatg cctatcccgt tatcagaaac
     3061 ctttaagatc ctgtcggttt tgtttgtctt gatttcgatc tcgaaattat ccctgttgat
     3121 gccactgctt ccttcttcgg tcagcatttt gtagtaaagt ttgtcgatag catcgcttcc
     3181 gttggatatc aactccctca aaaatatttc cttatgtgta taaatggagt tgatcataag
     3241 gtccaaaagt cttttggatt cagctttgaa ttgttttttt gccatttgtt atcactcctg
     3301 tttttaattt gtctgttatg acttgcttct cttggttagc actctcttgt tttgagtgct
     3361 aaaaacaata tacacctgct tctctttgca gtcaaggggg tttttgctca tatgggatca
     3421 aaaatgcaaa atcgttttaa cggacaactc aaaacttcgc accttttgcg gatgcgaagt
     3481 tttggtttta atacagtatg attatcagac cttccccaag accggtccct tattcgtttt
     3541 ctattttcct tatatgtttt gatcttaagg cagttcttgg gtggcggaaa tattgatgtt
     3601 ttccagtatc atttcattta catcatcata tacatcaagt attttctcct gataggtgtt
     3661 tttgtgtata aatgttatga ttttcttgat gttccttatt atatctgcgg ttcctgtatt
     3721 tgttgctttt ttcagttcct cttctgtgat cgcgatcagt gaatgtgcaa ctttatttct
     3781 atattcattt acatcatcaa atctgttgaa catctcaaag taatcgatat gattcagttt
     3841 tttcaagttc cccgcatatt ttttattatt ggcaaagaaa ttgattatct ctttcattac
     3901 gttaacactt atgtcactat ctctgaatcc atctctatat tttttgttta acctttcgcc
     3961 taattcttta taattgtttt ctatcttttt tgtgtcaaat atatatctgt ctttcttttt
     4021 tgatgcgatt gaatcttttt ttatattaaa tacattttct aaaaagctta tttgcagcct
     4081 tataaccaat ggattcagtc taatcaataa ttctgtcacc ttgcccattt tctgaagttg
     4141 cataagaata agcatatact cggttactct tttgcattcg ctatctttta taggataaaa
     4201 atcatagtca attatttctt tttgtatttc cacagagttc ttgagatttt gtctttcgct
     4261 taaatgactg atcaatttta ttacccgtga atcctttgtg aatccttgtg ccagtgcaaa
     4321 tttttcacac ccatcatagt tgtaatcata caaaagggct ctgagctgct cctttgccaa
     4381 acttcttttt aattcaatga acttcggctc gacacatctg ttttcacttt cttccaaatc
     4441 atcaaaatta ttgtccagcg cttcttgaaa atcgtaatct ttgcttgttg ttgtttttga
     4501 ggtgtttgag gcttttgccg gagacgaaac ctgtatcgct tttatattaa aactaatatc
     4561 ttgtgaaagc aaagccagtg taatgagcat ttgtggagtt cccgagctaa tattgaccaa
     4621 aagttccgca tccgaatgct tttctttgat atccttgaag tacccttcaa actcatggtt
     4681 aaatgcatca aaatccgata cgtcgacttt atcactgccc aaaggtatcg attcgatttc
     4741 atgtcctata tttccagtca cggtatttat tgccaaaaga atctcatctt ttctttttga
     4801 catttccttt gacatataaa aatacactgt ttccggtctg tagtgcctta ttatgtgcag
     4861 catcggtccg tcaaaatctc ctctgaccgg atcagaggca cccacacaag taaaaagaat
     4921 tcttttcatg atttctcctc caaaaataag ttacttaaag tataataata tcttctttta
     4981 tatctcttct attttctccc cagtattttc cttccccttc taacatatat acccttacat
     5041 tatcactgct ctttatgatc ctgggtattt tttttatcat ttttttatat ttactgctgt
     5101 ccagaaaagc ttcaaatgca gatttttgaa ctctgtagcc ata
//
LOCUS       DAONHK010000079         5021 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_12953_length_5021_cov_5.019936, whole genome shotgun sequence.
ACCESSION   DAONHK010000079 DAONHK010000000
VERSION     DAONHK010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5021
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12953_length_5021_cov_5.019936"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..858)
                     /locus_tag="PLM92_03900"
     CDS             complement(<1..858)
                     /locus_tag="PLM92_03900"
                     /inference="COORDINATES: protein motif:HMM:NF024252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPR24316.1"
                     /translation="MMFSQNLQYLRKEKKMTQEQLAEKLEVSRQAVSKWEAGSAYPEM
                     DKIIVMCRMFDIDMDTLVNGNAKKTVSEDDFGYDDFYNKCSRAVAFGVSLIIAGVAIL
                     IAMEGENHGKVGLLILFLLTTVAVVTFVWFGIQMDHFEKKHRSVPDFYKEEEKESFRK
                     KFALGMAAGVGLILLGVIIVIGSSLVDLTPHFAVSGMMICIAFAVYIFVYFGMQQQKY
                     NIEEFNEKNIKETSAKADKYSSIIMLSATAIFLLLGFLGGYWHPAWVVFPLGGIMCGI
                     VATALQKTKE"
     gene            complement(978..1976)
                     /locus_tag="PLM92_03905"
                     /pseudo
     CDS             complement(978..1976)
                     /locus_tag="PLM92_03905"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
     gene            complement(2237..3754)
                     /locus_tag="PLM92_03910"
     CDS             complement(2237..3754)
                     /locus_tag="PLM92_03910"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008370817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008765 -
                     UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HPR24317.1"
                     /translation="MEKNFYELKEYISLLEEEGLVVQVFSGEEDLKKRVRYISYSSKD
                     IEFNTLFICKGAHFSEEYLHEALGKGAVAYISENKYDVDAPCIKVEDIRKAMPLIADF
                     FYEKIWRRICIAGITGTKGKSTTAYYMKSILDQYLKVSKKPESAIISGIDNYDGIIKE
                     ESHLTTPEALMLHRHFDNAVKSGIEYISMEVSSQALKYHRTKGIVFDVACFLNLGTDH
                     ISDIEHNSFEDYSASKLIFFRQCKVACVNLDDDYADRVIEEAKKSRETEKIITFGLRE
                     DANIHGYDLKPGKKGIAFKVRSDSFDTEFEIGMTGLFNVSNALAAIAMSYALDIPLKY
                     IKSGLKKAKAAGRMEIFHNEDETLTVVVDYAHNQMSFESLFASTKKEYPGKKIFIVFG
                     CPGKKALGRRKELGDIAGKYADKSFITEEDAGEEPVHEISEEIASHIKEQGGNCVIID
                     DREEAIKRAINEADGDTVILITGKGRETRQKRGIEYIETPSDVEYVARFLKEKKC"
     gene            complement(3755..4468)
                     /locus_tag="PLM92_03915"
     CDS             complement(3755..4468)
                     /locus_tag="PLM92_03915"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HPR24318.1"
                     /translation="MKKEKTKCLGIIGGMGPLATQTFYKMIIDMTRADSDQDHIDMII
                     LNHASMPDRTKAVKEGHLDELFVKLVMDARFLENGGASFIAIPCNTSHVLINRLKDSV
                     DIPFINMIEESVREIRSLYGSGKKVGIMATDGTVKMGIYQEECLKNDLVPIVPSEKNQ
                     KRIMKIIYEGVKAGEAVDKKDFDVVKKEFDEKGCECALLACTELSCIKETEDLPGYYI
                     DAMGSLAKKAVELCGKELK"
     gene            complement(4472..>5021)
                     /locus_tag="PLM92_03920"
     CDS             complement(4472..>5021)
                     /locus_tag="PLM92_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009257904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HPR24319.1"
                     /translation="YSDRQGRVRMMCLGHVLLEEHTPHGIGNHAVIITEQNIELEEKI
                     KKMLETIGYTGFSNFDIKYDSRDGKFKAFEINTRQGRSNFYVTGSGANVAEYFVRDHI
                     YGENMGFYSVDAENLWMVVPKKVAFDYIREEYHDEMKRLIREGKYQNPLIYAPDNKLK
                     RKLRTTKLLMGHHNKFKKYLGK"
BASE COUNT         1275 a         1207 c          870 g         1669 t
ORIGIN      
        1 ttcttttgtc ttttgtaatg cggtggcaac gataccgcac attatgccgc ccagcgggaa
       61 aactacccat gcgggatgcc agtatccccc cagaaaaccg agtaaaagga atattgccgt
      121 ggcagacagc attattattg aactgtattt gtccgccttt gccgatgttt cctttatgtt
      181 tttttcgttg aactcttcaa tattgtattt ctgctgctgc atgccaaaat atacaaaaat
      241 ataaactgca aaagcaatgc atatcatcat acctgaaacg gcaaaatgag gtgtaagatc
      301 aaccagcgaa gaccctatca caataataac tcccagaagt atgagcccta ctcccgctgc
      361 catacccaat gcaaattttt ttctgaaact ctctttttct tcttctttat agaagtccgg
      421 aacggatctg tgcttttttt caaaatgatc catctgtatc ccgaaccaaa cgaaagtcac
      481 aacagcaact gttgtcaaca ggaaaagaat cagtaatcct acttttccgt ggttttctcc
      541 ttccatggct atcaatatgg ccactcccgc tattatcagt gataccccaa aggctacagc
      601 tctgctgcat ttgttataaa aatcatcata accaaaatca tcctccgata cggttttttt
      661 tgcattgccg tttaccaggg tatccatatc gatatcaaac atcctgcaca taactattat
      721 cttatccatt tccggataag cagatccggc ttcccacttg gataccgcct gtcttgaaac
      781 ttccagtttc tccgccagct gttcctgtgt catttttttc tcttttctca aatactgcag
      841 attttgtgaa aacatcattt ttcctttcct cccaaaaaat catcacgtgt aaaaaacgtt
      901 tttattctct gagataatga tacggttgcg gacaacagac cactaccatt tttatgtttc
      961 tttctatttt tttttcgcta cctgtggttg cttttgagcc atggagccgg aataagagat
     1021 gcaatatagg ccgctactgt caaaggcaca ataagcctta agggaaagca aaggaaaagt
     1081 accgccacca cggtaagagg ttttctcatg atatatccat aggcggcagc cgaaacaacg
     1141 gcaacggcaa acaccggttc aacacctgag acagcggcaa gggcgtatcc tatggaaact
     1201 cccgaaaaaa tgaccggaaa tatgttcccg cctttccatc cgaaatttac acatatattt
     1261 acagcaagaa gttttcctat ggcagtaagg atcagtaatg ttaccggtat gttctcccag
     1321 ttttccataa gtatgcccat ttcgtgttca ccgctgaaca tggtccatgg aagccatatc
     1381 cccagcatgg ccattataaa ccctgcgatc gtgcagctta atatcctgtg ttttattatt
     1441 cgtttcccca gggtttttgt tattttgtta aaggtgaggt acaaaagacc gcacaacaat
     1501 ccggcagcag caaaaactac gaaccatttc cactcctgta tcccggcatt tttttccgca
     1561 gtaaatctgg caagccccag tcctccgcca aaaaagttct taaggagcat cattgtccca
     1621 aagccgcccg ctatgcctgc cgcatatacg atggcttttt cgaccttgaa ctactttggt
     1681 gaaaaatatt ttttttcgtc ttcccttggt ttttcataat tatttacgaa accaaaaagg
     1741 ggggcttcga atataactcc caatgtagcc gccattccgg ctcttgccag atccttcgct
     1801 tcctcccctt tgtatttaag tttgtctccc gtccagcagc aaagaccggt aacaaggccc
     1861 gtcaaccctg cttctggccc aagggaccct ccgaagatca gtggaagaag agctgctgta
     1921 gctatgatat aaagcttatc gtaagaatag gtcccctccc ttttcagttt tatcatggtt
     1981 tcttccagag tatcgggaag tatcccgtat ttcctctgaa aaaaacctat gacaagtccg
     2041 cccagggtac agacggctgt ttggtatata taataatttg ttccgtaact gattttgtcc
     2101 ggtattttct gccaaatgat atctatacag aaattcatta tataaagtac cagccatatt
     2161 atggccccgg aaacagctcc gaggacaagc gcataaaagg caaacaacaa tgtgtttttt
     2221 attttttgtg ttcttttcag catttctttt ctttgagaaa ccttgcaaca tattcaacgt
     2281 ctgaaggtgt ttcaatgtat tcgatgccgc gcttctgtct ggtttctctt cctttaccgg
     2341 tgatcagtat cacagtatct ccatccgctt cgtttattgc ccttttgatc gcttcctctc
     2401 tgtcatcgat tattacacag ttgccgcctt gttcttttat gtgtgaggct atctcttcac
     2461 ttatctcatg cacgggttct tctcccgcat cctcttctgt aataaagctt ttatctgcat
     2521 atttccctgc aatatctccc agttcttttc ttcttccaag agcttttttg cccggacatc
     2581 cgaaaactat aaatattttt ttgccggggt actctttttt tgtggatgca aaaagagatt
     2641 caaaactcat ttgattgtga gcataatcaa cgaccactgt aagcgtctcg tcttcattat
     2701 ggaatatttc catacgaccg gcggctttgg cctttttcaa accgcttttt atgtatttca
     2761 ggggtatatc aagagcatat gacatggcta tggccgcaag ggcattgctc acattgaaaa
     2821 ggcctgtcat tccgatttca aactccgtat cgaaactgtc gctcctgact ttgaatgcta
     2881 tgcctttttt ccccggtttc agatcgtatc catgtatatt tgcatcttca cgcaatccaa
     2941 aagtgattat tttttccgtc tcccggcttt tttttgcttc ttctatgact ctatcggcat
     3001 aatcatcatc cagatttacg catgctactt tgcactgccg gaaaaagata agttttgaag
     3061 ctgaataatc ttcaaaacta ttatgttcta tatcgcttat atgatctgtt cccagattca
     3121 aaaaacaggc tacatcaaat actattccct tggtcctgtg atactttaga gcctggctgg
     3181 aaacctccat gcttatatat tctattccgc tttttaccgc attatcaaaa tgtctgtgca
     3241 gcataagggc ctcaggagtg gtcaggtgag attcttcttt tattatcccg tcataattat
     3301 ctattccgga aataatggca ctctcaggtt tttttgagac cttcaggtac tgatccagta
     3361 tgcttttcat ataatatgca gtggttgatt tgcctttagt ccccgttatt cccgctatac
     3421 aaatgcgtcg ccatatcttt tcataaaaaa agtctgctat caaaggcatc gcttttctta
     3481 tatcctctac tttgatgcac ggagcatcaa catcgtattt attttcactt atatatgcaa
     3541 ctgcgccttt tcccagtgct tcatgaagat actcctccga aaaatgggct cctttgcata
     3601 tgaataaagt attgaactca atatcctttg aactgtatga tatatatctg actctttttt
     3661 taagatcctc ttctccggaa aagacctgaa caacaagtcc ttcttcttcc agcagggaaa
     3721 tatattcttt caactcataa aaattttttt ccatttattt gagctccttt ccgcaaagtt
     3781 caacggcttt ttttgccaaa gatcccatgg catcaatgta gtaaccgggc aggtcttccg
     3841 tctccttgat gcatgaaagc tctgtacagg ccagcagtgc acattcacat cctttttcgt
     3901 cgaattcctt tttcactaca tcaaaatctt ttttgtcgac tgcttcaccg gcttttactc
     3961 cttcgtatat tatcttcatg atccttttct ggtttttttc tgaaggaact atgggcacca
     4021 ggtcgttttt aaggcactct tcctgatata tgcccatttt gaccgtgccg tccgtggcca
     4081 ttataccgac ttttttgccg ctgccgtaaa gactccttat ctctcttacc gattcttcaa
     4141 tcatgtttat aaaagggatg tctacagaat ccttaagcct gttgatcaga acatgggaag
     4201 tgttgcaggg tatggcgata aaagaggctc cgccgttttc aagaaatctt gcatccatga
     4261 ccagcttgac aaaaagttca tcaagatgcc cctctttgac ggcttttgtt ctatcgggca
     4321 tagacgcatg gttaaggatt atcatgtcta tatgatcctg atcggaatct gccctggtca
     4381 tatctattat cattttataa aatgtctgtg tggcaagagg tcccatccct ccgattatcc
     4441 ccagacactt ggttttttct tttttcataa atcatttccc caaatacttc ttgaatttat
     4501 tatggtgtcc cataagaagt ttcgttgtcc tcagttttct cttcaatttg ttgtcaggtg
     4561 catatatcag cggattttga tatttgccct ctctaatgag tctcttcatc tcatcatggt
     4621 attcttctct tatataatca aatgccacct ttttgggcac gaccatccaa agattttctg
     4681 catcaacgga ataaaacccc atgttttctc cgtagatatg gtcacgtaca aagtattccg
     4741 ccacatttgc tccggaaccg gtaacataaa aattgctgcg tccctgcctt gtgtttattt
     4801 caaatgcctt gaattttccg tctctgctgt catattttat gtcaaaattc gaaaatccgg
     4861 tatatcctat cgtttccagc atttttttta ttttttcttc cagttctatg ttctgctccg
     4921 taattatgac cgcatggttg cctatgccat ggggcgtatg ctcttcaaga agcacgtggc
     4981 caagacacat catcctgact ctgccctgtc tgtcggaata a
//
LOCUS       DAONHK010000080         5012 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_12994_length_5012_cov_4.076659, whole genome shotgun sequence.
ACCESSION   DAONHK010000080 DAONHK010000000
VERSION     DAONHK010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 5012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5012
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12994_length_5012_cov_4.076659"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="PLM92_03925"
     CDS             <1..375
                     /locus_tag="PLM92_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HPR24320.1"
                     /translation="EHAELWFKLAHGIGTTEENLLDAAAGEHYEWTEMYKEMAETARE
                     EGFDEIANQMEGVAAIEKEHEERYRKLAENVKAGKIFEKEEEVLWQCSNCGHQFKARK
                     APDICPVCLHPKAYFQVKPENF"
     gene            complement(372..1064)
                     /locus_tag="PLM92_03930"
     CDS             complement(372..1064)
                     /locus_tag="PLM92_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014355838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPR24321.1"
                     /translation="MIKIKNLTVKYPDDTEALRSLDLSISEGESIALMGANGAGKSSL
                     LLAITGIAPYNEGEIYVDDILLSKKTVADVRQRVGMIFQDPDDQLFMPSVFDDVVFGP
                     RNKGLDEKETVRIAESVLEALMISHLSSKVPGKLSGGEKKLVALAGVLAMDPSNLLFD
                     EPAAFLDPGAKRRLVSLVGNLDHTKIIATHDSDIARQLCSRVIILKKGSLSYDGTPDI
                     LDDLSVLEKYGL"
     gene            complement(1061..1861)
                     /gene="cbiQ"
                     /locus_tag="PLM92_03935"
     CDS             complement(1061..1861)
                     /gene="cbiQ"
                     /locus_tag="PLM92_03935"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0032778 - P-type cobalt transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HPR24322.1"
                     /translation="MQGLTKATYGFHRLDSLARGNTVIHKIDPMTKTITTLIYVVIVV
                     SFDRYDLSGLMPFVFYPVILMVLSETPLKPLMTRMAIALPFCIFAGAANLIFEKETAF
                     FMGSVPVSFGMISFFSIILKTILTVTAVLILAATTPMAQISKQFVRLKVPSLIVLLFS
                     MIYRYINTALQETISMYTAYKLRAPGEKAVKMKDMGAFTGQLFLRSADRSERIYAAMK
                     CRGFSGDYDHIRSGAVPAGEWFYIVILTISLLALRFFNVSLFIGGVLS"
     gene            complement(1849..2829)
                     /locus_tag="PLM92_03940"
     CDS             complement(1849..2829)
                     /locus_tag="PLM92_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014355836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HPR24323.1"
                     /translation="MHMADALVSPAVGVTMAAVSVGAIAYSVVKMKKEEDFDEKKVPL
                     MAVSGAFVFAAQMINFTIPGTGSSGHIGGGILLAALLGPYPALLTISAVLLIQCLFFA
                     DGGLLALGCNIFNMGIIPCLFAYPLIFKPIAGKVLSNGRIMAASVVAVVIGLQLGAFG
                     VVLETSASNVTALPFGTFLLLMQPIHLAIGIVEGIVTGMVLVFVRSMRPEILKCSSEK
                     KSLEDVSIKKTIIVFSAAALLIAGILSIFASSFPDGLEWAMEKTAGTAELANNGAFKD
                     AAGSFQSQTAFMPDYDSGAAGIVGGLITVILTGITGLIIYLVKRKKTCKA"
     gene            3081..3923
                     /locus_tag="PLM92_03945"
     CDS             3081..3923
                     /locus_tag="PLM92_03945"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HPR24324.1"
                     /translation="MRDVFVNKITDAVASLARETNYYLGEDVCKRISQCREAEDWPPA
                     KDILAKIEENIKTAGEDGIPLCQDSGLACVFLEIGQDVHLTGGRIEDAVNEGVRKGYE
                     EAYLRKSVVSDPLRRTNTNDNTPATISYDIVPGDRVKVIFAPKGFGAENMSRIAMLAP
                     SEGEQGVKDFVVETVEKAGGNPCPPVVVGVGIGGTFDKVTLLAKKALLLPLDLPNRDP
                     YYAQMEEELLKRINGSGTGPQGFGGKTTALAVKILTAPTHIAGLPVAVNLNCHVARHG
                     EIII"
     gene            3935..4492
                     /locus_tag="PLM92_03950"
     CDS             3935..4492
                     /locus_tag="PLM92_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012425205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing hydro-lyase"
                     /protein_id="HPR24325.1"
                     /translation="MTVKISGPFTKEKLVCLRAGDDVLLSGEIYTARDAAHKRMTEML
                     KRGEELPFDIENSIIYYVGPTPAKPGRPMGSAGPTTSYRMDPYAPMLMEKGQTGMIGK
                     GPRSEEVIEAIKKNKAVYFAAIGGAGALMSKKIIRSETVAFEDLGTEALRRLTVLDMP
                     VTVAVDSFGNDLYKTGRKKYLEEQK"
     gene            4489..>5012
                     /locus_tag="PLM92_03955"
     CDS             4489..>5012
                     /locus_tag="PLM92_03955"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005837953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HPR24326.1"
                     /translation="MIDLHYLKLLSEQYPNRKAAAAEIINLKAILALPKGTEYFLSDL
                     HGEHEAFIYMLKSASGVIRRKIEEIFGQDLTRAERDELASLIYNAEAEIKRRKKSEKD
                     FDKWCYLSIYKLIQVCRAVSTKYTRSKVRKRLPEHWGYSMDELLHADDEENKSRYYSK
                     IIESIIESRIAEDF"
BASE COUNT         1584 a         1023 c         1168 g         1237 t
ORIGIN      
        1 gaacatgctg aactctggtt caaactggct cacggcatag gcacaacaga agaaaatctg
       61 ctggatgcag cagccggaga gcattatgaa tggacagaaa tgtacaaaga aatggctgag
      121 actgcaaggg aggaaggctt tgatgagata gcaaatcaga tggaaggcgt agcggccatt
      181 gaaaaagagc atgaagaaag atacagaaaa ctggccgaaa atgtaaaagc cggaaaaata
      241 tttgaaaaag aagaggaagt tctgtggcag tgttccaact gcggacatca gttcaaagcc
      301 agaaaggctc cggatatatg tcccgtatgc ctgcatccaa aagcatattt ccaggtgaag
      361 cccgagaatt tctagagacc gtatttttca agaacagaaa gatcgtcaag tatatcaggg
      421 gtcccgtcat atgacaggga tccctttttc agtatgatga ctctgctgca aagttgccgt
      481 gcgatatcag aatcatgcgt agctattatt tttgtatgat caaggttccc gaccagggac
      541 acaagtcttc tttttgctcc cggatccaga aaggcagcag gctcgtcaaa gagtaggttt
      601 gaagggtcca tggcaagaac tcctgccaga gccacaagct ttttttcgcc tcccgaaagc
      661 tttcccggaa cctttgaaga aagatgggat atcataaggg cttcaaggac agattctgcg
      721 atccggactg tttctttttc atccagtcct ttgtttcggg ggccaaagac cacatcatca
      781 aaaaccgaag gcataaaaag ctgatcatcc ggatcctgaa aaatcatgcc gactcgctgt
      841 cttacatcag ccactgtttt ttttgaaaga aggatgtcat ccacatatat ctcaccttcg
      901 ttatagggtg ctattcctgt tattgccagc aaaaggctgg atttgccggc accgttggca
      961 cccataaggg caatactttc gccttcgctt atagaaagat ccagagacct cagagcctcc
     1021 gtatcatcgg gatatttgac cgttagattt tttattttta tcaacttaaa acacctccga
     1081 taaaaaggct tacattgaaa aaacgcaaag ccaaaaggga aatcgttaga atgacaatat
     1141 aaaaccactc ccctgcagga acagcaccgg atcttatatg atcataatct cccgaaaaac
     1201 ccctgcactt cattgccgca tatattcttt cgcttcgatc tgcagatctc aaaaaaagct
     1261 gtcctgtaaa agcacccatg tctttcattt taacggcttt ttctcccgga gcacgaagct
     1321 tgtaggccgt gtacatgctt atggtttcct gaagagcggt gtttatatat ctgtatatca
     1381 tggaaaagag aagaactatc agagatggta ctttcagtct tacgaactgt tttgatattt
     1441 gagccattgg tgtggttgca gccagtatca gaaccgcagt aactgtaagt atggttttca
     1501 gtataataga aaaaaatgat atcattccaa aggagacggg gacacttccc ataaaaaaag
     1561 cagtttcttt ttcgaagatc agattagctg caccggcaaa aatacaaaat ggcagggcta
     1621 tggccatcct ggtcataagg ggttttagcg gtgtttcaga taaaaccatc agtatgacag
     1681 gataaaaaac aaagggcatg agtccgctga gatcatacct gtcgaatgaa acaacaataa
     1741 ccacatatat caatgtggtt attgtttttg tcataggatc aattttgtgt attaccgtat
     1801 ttccacgggc aagagaatca agccggtgaa aaccgtatgt ggcttttgtc aggccttgca
     1861 tgtttttttc cttttcacca ggtatattat caatccggtt attcctgtca agatcactgt
     1921 gataaggccg cctactatac ctgcggcacc tgagtcataa tccggcatga aagctgtttg
     1981 actttgaaaa gaaccagcgg catctttgaa agcgccattg tttgccagtt ctgctgtacc
     2041 tgcggttttc tccattgccc attccagtcc gtccgggaaa gaagatgcaa atatcgacag
     2101 tatacctgcg ataagcaggg ctgcagctga aaagactatg atagtctttt ttatggaaac
     2161 gtcttcaaga gatttttttt ctgaagagca ttttaatatt tccggccgca tggatctgac
     2221 aaaaactaat accattccgg taacgattcc ttctactata cctattgcca gatgtatggg
     2281 ctgcataaga agaaggaaag tgccgaaggg aagagctgta acattcgagg ctgaggtctc
     2341 aaggaccaca ccaaaggcac caagctgcag gccgatcact acggcaacaa ctgaggccgc
     2401 cataatgcgt ccgttgctca gaacctttcc tgctatgggt ttgaaaataa gaggatatgc
     2461 aaaaaggcat ggtataatac ccatattgaa aatgttacat ccaagagcaa gcaggccgcc
     2521 gtcggcgaag aacaaacact gtatcagcag cactgccgat atggtcagca atgccggata
     2581 aggaccgaga agtgccgcaa gcaatatacc gcctccgata tgtccactgg agccggtgcc
     2641 gggtattgta aaattgatca tttgtgccgc aaacacaaat gcaccggata ctgccataag
     2701 aggaactttt ttctcgtcaa aatcttcttc ttttttcatt tttacgaccg aatatgcgat
     2761 cgcacctaca cttactgcgg ccattgtaac acccactgcc ggtgatacca gagcatctgc
     2821 catgtgcatg atatgtctcc ttttctataa ataaaaaacc agaaaaaacg aataatctgc
     2881 tgctgcagtg atccaccttc ctggtgacaa attacgagta aacattgtta ttagctgtat
     2941 atttgtgcat tgcaccttca tgatgcaaca aggttattat aggtcaaaga gcagatcatg
     3001 tcaatgatat tgaaaaccat tttgtattat agatgcaata tggtataata aacctgcaag
     3061 tggattccgc gaggacgaaa atgagagatg tttttgtaaa taaaataacg gatgcggtgg
     3121 catctttggc ccgggagacc aattattatc tgggagagga tgtttgtaag cgtatttccc
     3181 agtgcaggga ggctgaagac tggccccctg cgaaggatat acttgcaaaa atagaagaaa
     3241 atatcaaaac agcaggtgaa gacggtatcc ccctttgtca ggacagcgga cttgcctgtg
     3301 tttttttgga aataggccag gatgtgcatc tcacaggcgg aagaatagaa gatgccgtaa
     3361 atgaaggtgt tcgtaaaggg tacgaagaag catatcttag gaaatcagtt gtatcggatc
     3421 ctttaagaag aacaaatacc aatgacaaca ctcctgcgac cataagctat gatatagttc
     3481 ccggagacag ggtgaaagtg attttcgcac caaaaggatt cggtgcggaa aatatgagca
     3541 ggatcgcaat gcttgctcct tcggaaggag agcagggagt aaaggatttt gttgttgaga
     3601 cagttgaaaa ggcaggagga aacccatgcc ccccggtggt ggttggagtg ggtataggcg
     3661 gcacattcga caaggtcact cttttggcga aaaaggcact tttgctgcct cttgatttac
     3721 caaacagaga cccctattat gcccaaatgg aggaagaatt gctgaaaagg ataaatgggt
     3781 caggaaccgg tcctcagggt ttcggaggga aaaccactgc tctggcggtg aaaatactaa
     3841 cagctcccac tcatatagcg gggctgcctg tggcggttaa ccttaactgc catgtggcaa
     3901 ggcacggaga gataataata taaaaggtaa aaaaatgaca gtgaaaatca gcggtccatt
     3961 tacaaaagaa aaactggtct gtctcagggc aggagatgat gttcttttgt ccggggaaat
     4021 atatacagca agggatgctg cacataagcg gatgacagaa atgctcaaaa gaggagaaga
     4081 gcttcctttt gacatagaaa attcaatcat ttattatgtg ggtccgacac cggcaaaacc
     4141 gggcagacca atgggctcgg caggcccgac caccagttac agaatggatc cttatgcacc
     4201 catgcttatg gaaaaagggc aaacggggat gattggcaaa gggccgagaa gtgaagaagt
     4261 aatagaagct atcaaaaaaa acaaagcggt ctattttgcc gcgataggcg gagcaggagc
     4321 tctgatgtca aagaaaataa taagatccga aacggtagct ttcgaagatc ttggaacgga
     4381 ggcactgcgc agactcacag ttttggatat gcctgttaca gtggcagtgg actcttttgg
     4441 aaacgatctt tataaaacag gaagaaaaaa atatctggag gaacaaaaat gatcgatctg
     4501 cattatctta agcttctgtc tgaacagtat ccaaacagaa aagcggctgc agccgaaata
     4561 ataaatctga aggcaatact ggcgcttccc aagggaacgg aatattttct cagcgatctt
     4621 cacggagaac atgaagcctt tatctacatg ctcaaaagcg catccggagt aataagaaga
     4681 aaaatcgaag agattttcgg tcaggacctt acaagagcag aaagagatga attggcttct
     4741 ctgatatata atgcggaagc agaaataaaa agaaggaaaa aatccgagaa agattttgac
     4801 aaatggtgtt acctgtccat ttataaactt atacaggtgt gcagagccgt atccactaaa
     4861 tacaccagat caaaggtgcg taaaaggctg cccgaacact ggggttacag catggatgaa
     4921 cttttgcatg ccgatgacga agaaaacaag tcaagatatt acagcaagat aatcgaatca
     4981 ataatcgaaa gccgtatagc agaggatttc at
//
LOCUS       DAONHK010000081         4991 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_13080_length_4991_cov_4.021677, whole genome shotgun sequence.
ACCESSION   DAONHK010000081 DAONHK010000000
VERSION     DAONHK010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4991
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13080_length_4991_cov_4.021677"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="PLM92_03960"
     CDS             complement(<1..523)
                     /locus_tag="PLM92_03960"
                     /inference="COORDINATES: protein motif:HMM:NF013942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HPR24327.1"
                     /translation="MKEKVKLLVIFGGISSEHDVSCESAASIIKRLDRKKYDIYKIGI
                     TKGGRWILTDAETEKIADGTWENRISNRMAAVSPDRTLHGIRTLNGEEIHIDCVFPVL
                     HGRFGEDGTMQGLLELAGIPYVGPGVLSSSCCMDKTVTKDLISREGFLQPRYYVTDRY
                     EFSSDPKGILADIE"
     gene            complement(544..1272)
                     /locus_tag="PLM92_03965"
     CDS             complement(544..1272)
                     /locus_tag="PLM92_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009304082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPR24328.1"
                     /translation="MEKPVIEFKNVTKRYKLFKNDKKRLMSVFIKKIKYKEKKAVDDV
                     SFDIMKGEAVAFFGKNGAGKSTILKIITGVTYPTEGSVKVHGRVSALLELTSGFDPEL
                     TGRENIFLKGQLMGLKDKEIKALENTIIEFAELGDYIDQPIRTYSSGMKSRLGFAINA
                     NIKPEILIVDEALSVGDASFRKKCVKKVNEIMESEGLTLLFVTHSASDAKEFCKRGIV
                     LKNGKLIHDSDIEDSIKKYKELLT"
     gene            complement(1294..2097)
                     /locus_tag="PLM92_03970"
     CDS             complement(1294..2097)
                     /locus_tag="PLM92_03970"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPR24329.1"
                     /translation="MELFRIIKEHIAWRHQIFKLAKADIIKTYSGAALGWSWAVIKPS
                     VTIFVFWFAFSVGLRTRGPEGYSFFLWLISGMVAWFYISDMWNQGTGAMRKYSYLITK
                     MKFPISTIPTFVSISKLVIHLGLLAITIVLFMLAGHMPDKYFIQLPLYLLLMFLMATG
                     WALFASVMAAMSKDFWNLVKAFSTAIFWLSGIMWDPYSPAIPYWVKNFLMFNPVTYIV
                     CGYRDCFIYERWFFEQPLLLAIFLGEMIIMWTFAIWAYKKLKEDLPDVL"
     gene            complement(2103..3200)
                     /locus_tag="PLM92_03975"
     CDS             complement(2103..3200)
                     /locus_tag="PLM92_03975"
                     /inference="COORDINATES: protein motif:HMM:NF015755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HPR24330.1"
                     /translation="MRTDKINTYENIVPENKRQKRRRWSKKRKALIIISVLVIVCVAL
                     LIGGYFFLLDKFNNMDYEDLNGADYDINPGVEEKLEGYRNILIVGLDTRENEDESYSK
                     SDAIIIATIKEDNGKVRLTSVYRDTLLDLEEQKGKHRLDKLTHQYMYGGPLSLIRTLN
                     RNMDLNIKEYVKVNWETVADATDNMGGLELTIKDYEIKEMNKYIKSTNKTLHGDKTKI
                     KKAGKQTLNGIQTVTYCRIRKVGNGDYERTERMRKTMKAAFKKTKTMSLGELNDLAEE
                     ILPEIGTNITGKKIVGLAVGTLRYDLGKNSGWPYDVSEAVVDGVWYGPPVTLESNVKE
                     MHRKLFGQKNYEPTKKVKTINDRIIELTGVE"
     gene            complement(3197..3865)
                     /gene="rpe"
                     /locus_tag="PLM92_03980"
     CDS             complement(3197..3865)
                     /gene="rpe"
                     /locus_tag="PLM92_03980"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006308920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HPR24331.1"
                     /translation="MIKLAPSILSADFSKLGVDVAEIEKGGAHLIHVDVMDGHFVPNI
                     SYGATVMKSLLGKTEMPFDVHLMIEEPQKYIDDFMTEKTEYITVHQETCPGIPEIIKR
                     IKRAGAKAGVSINPETSEETVMDVLDSVDMVLIMSVHPGFGGQVFIESALKKAKTLAE
                     VKKKNGLSFKIEMDGGINLSNVKRVIESGTEIIVAGSSIFKTKNIEEATKRFLDIMNK
                     EEQS"
     gene            complement(3888..4751)
                     /gene="rsgA"
                     /locus_tag="PLM92_03985"
     CDS             complement(3888..4751)
                     /gene="rsgA"
                     /locus_tag="PLM92_03985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HPR24332.1"
                     /translation="MKGIIIKGIAGFYYVKCDGDIYRCRARGIFKKKEVVPLVGDIVE
                     ISIDENSDGTVEKIFPRDNFFIRPPIANIEVLAVMVSAAEPPPSTFILDRFLVMAEKH
                     NVRPVICINKKDLAKGNEIEELKSIYKEIYPVICLCGLTGEGIDLLKDFFRGRRVAFA
                     GPSGVGKSTILNRLLKEERAKTGEISIKTGRGKQTTRHVEIFDINDGMVYDTPGFTSF
                     NILDADEDELSHLYPEMKEYLNKCRYDNCRHVAEPGCSVREAVELGIIKRSRYDSYVE
                     QIKEIREKKKY"
     gene            complement(4765..>4991)
                     /locus_tag="PLM92_03990"
     CDS             complement(4765..>4991)
                     /locus_tag="PLM92_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPR24333.1"
                     /translation="TSTIAVDMSYSKAKQEIFYLTVTVSDSNGTRTVISGEQRQKSDG
                     GETVNISGTGSGTITVIFDDETVMTKKVSF"
BASE COUNT         1208 a         1198 c          870 g         1715 t
ORIGIN      
        1 tttctatatc tgccagtata cccttaggat ccgatgaaaa ttcatatctg tctgtgacat
       61 aataacgggg ctgcaaaaat ccttctctgg aaataaggtc cttggtcaca gtcttgtcca
      121 tacagcacga agatgacagt acaccgggtc ccacatatgg gatgcccgcc agctccaaga
      181 gtccctgcat ggtcccgtct tcaccaaatc ttccgtgcaa tacggggaac acacagtcaa
      241 tatgtatttc ttctccgtta agggtcctta taccgtgcag agtcctatca ggagaaaccg
      301 cagccattct gttgcttatc ctgttctccc aggtaccatc ggctattttt tctgtttcgg
      361 catcggtcag tatccacctt ccgcctttgg ttatacctat tttatatata tcatatttct
      421 tccggtcaag tctttttatt atggatgctg ccgactcaca tgagacatcg tgttcggatg
      481 atatccctcc gaaaatgacc agtagcttta ctttttcttt cattgatact acctgcatac
      541 tatctacgtt aacaattctt tgtatttttt gatggaatct tctatgtccg aatcgtgtat
      601 aagcttgccg tttttaagca ctattcccct cttacagaac tcttttgcat cactggcgga
      661 atgggtgaca aaaagtagcg taagcccctc cgactccatt atctcattta cttttttaac
      721 gcattttttt ctgaatgacg catcccccac agaaagagcc tcatcaacta tcagtatctc
      781 aggctttatg ttggcattta tcgcgaatcc cagtcttgac ttcatgccgc tggaatatgt
      841 ccttatgggc tggtcgatat aatcgcccag ttcggcaaat tctattattg tattctccag
      901 agcttttatc tctttatctt tgagccccat aagctgtcct ttcaggaaaa tgttctctct
      961 acccgtaagt tcagggtcga atcccgatgt gagttccaaa agagcactta ctcttccatg
     1021 gacttttaca ctgccctctg tgggatatgt cacacctgtt attattttca gtattgttga
     1081 tttacctgct ccgttttttc cgaagaaggc caccgcttct cctttcatga tatcaaaaga
     1141 aacgtcatcc actgcctttt tttctttgta ttttattttc tttataaata cagacatcag
     1201 gcgttttttg tcgttcttga aaagcttata tctttttgtg acatttttga attctatgac
     1261 cggtttttcc attatctaca ctttctcctg ttactataac acgtcaggca ggtcttcttt
     1321 aagttttttg tatgcccaga tagcaaacgt ccacattatt atcatttctc cgagaaaaat
     1381 ggccagaagc aatggctgtt caaaaaacca tctttcatat ataaaacagt ctctgtatcc
     1441 gcatactata taagtgaccg gattgaacat cagaaaattt ttcacccagt aaggtatggc
     1501 gggagaataa gggtcccaca ttattccgga aagccagaat atggccgtgc tgaaggcttt
     1561 gacaagattc cagaagtcct tgctcatggc cgccatgaca gatgcgaaaa gagcccagcc
     1621 cgtagccata agaaacatca gaagcaaata taagggcagc tgtatgaaat acttgtccgg
     1681 catatggccg gccaacatga acagtacaat ggttatcgca agaagtccca agtgtataac
     1741 aagcttggaa atactgacaa atgtagggat ggtagatatg gggaatttca ttttagttat
     1801 aaggtaactg tatttcctca tggctccggt gccttgattc cacatatcac ttatataaaa
     1861 ccaggctacc atacctgata taagccagag aaaaaatgaa tatccttcag gccctctggt
     1921 cctcagccct acagaaaaag caaaccagaa aacaaaaatt gttacagaag gcttgatcac
     1981 ggcccatgac catcccagtg ccgcaccgct gtaagttttt attatatcag cctttgccag
     2041 tttgaatatc tgatgcctcc atgctatatg ctcttttatt attctaaata attccatggg
     2101 ttctattcaa ctcctgtcaa ttctattata cggtcattta tggttttgac tttttttgtg
     2161 ggctcatagt ttttctgtcc gaataatttc ctgtgcattt ctttgacatt gctttccagt
     2221 gtcaccggag ggccgtacca tactccgtca acaacagcct cggagacatc atagggccat
     2281 ccggaatttt tgcccagatc atatctcagt gtccctacag caagtccaac gatttttttg
     2341 ccggttatat ttgtccctat ttcaggaagg atctcttctg caagatcatt gagctctcca
     2401 agagacatgg tttttgtctt tttgaaggcg gctttcatgg tcttgcgcat tctttctgtt
     2461 ctttcgtaat ctccattgcc cacttttctt atcctgcagt atgtaactgt ctggatgccg
     2521 ttaagagtct gtttccctgc tttttttatc tttgtcttgt caccgtgaag agttttgttt
     2581 gtactcttga tgtatttgtt catttccttg atctcataat cttttatggt gagttcaaga
     2641 cctcccatgt tgtctgtagc atccgccacg gtttcccagt tgactttcac atactctttt
     2701 atattgagat ccatattacg gttgagtgtc cttattagtg acagggggcc tccgtacata
     2761 tactgatgag tcagcttatc aagtctatgc ttgcctttct gctcttcaag atccagaagt
     2821 gtgtctctgt atacagaagt caaccttact tttccgttat cctcttttat tgtggcgatt
     2881 attatggcat ctgattttga atatgactcg tcttcgtttt ctcttgtatc aagacctact
     2941 atcagtatat tacggtaacc ttccagcttt tcctcaacgc cgggatttat atcataatct
     3001 gctccgttca ggtcttcata atccatatta ttgaatttgt ccaacaaaaa gaagtacccg
     3061 ccaatcagaa gtgccacgca aacgatcacc aatacggaaa ttattattaa tgcctttctt
     3121 ttttttgacc agcgccgtct cttttgtctt ttgttctccg gcacaatatt ctcgtaagta
     3181 tttattttgt ctgttctcaa ctctgctcct ctttattcat tatgtccaaa aacctttttg
     3241 tggcttcttc tatgttcttt gttttaaaaa tagacgaacc cgccacgatt atctctgttc
     3301 ccgactcaat aactcttttt acatttgaaa ggtttattcc tccatccatt tctattttga
     3361 atgaaagccc gtttttcttt ttcacttctg ccagggtttt tgccttctta agcgccgatt
     3421 caataaatac ctgccctccg aaaccggggt ggacagacat tatgaggacc atatccacac
     3481 tgtcaagcac atccatcact gtttcttcgg aagtttcggg atttattgat accccggcct
     3541 ttgccccggc ccttttgatc ctttttatta tctccggtat ccccgggcat gtctcctgat
     3601 gtactgttat atactctgtt ttttctgtca tgaaatcatc tatgtatttt tgaggttctt
     3661 ctatcataag atgtacatca aagggcattt cggttttgcc cagaaggctc ttcataacag
     3721 ttgcaccata gcttatattg ggcacaaaat ggccatccat aacatccaca tgtatgagat
     3781 gcgctccacc cttttctatt tcagccacgt ctactccaag ttttgagaaa tccgccgaca
     3841 agatcgacgg ggcaagtttt atcatttttc accttccctg ctgagtatca gtattttttc
     3901 ttttccctta tttcttttat ttgctcaaca tatgagtcat atctggatct ttttattatg
     3961 cccagctcta ccgcctccct gacagaacag cccggttctg caacatgcct gcagttatca
     4021 tatctgcatt tgttaaggta ttctttcatc tcgggatata ggtgcgagag ctcatcctca
     4081 tcggcgtcca aaatattaaa agatgtgaaa cccggtgtat catagaccat cccgtcattt
     4141 atgtcaaata tttcaacgtg cctggtagtc tgtttccctc ttcctgtctt tatgcttatt
     4201 tctccggtct ttgctctttc ctcttttagc agcctgttta atatggtgga tttcccaacg
     4261 cccgatggac ctgcgaaagc aactcttctg ccccgaaaaa aatctttcag cagatcaatc
     4321 ccctctcctg tcagaccaca tagacatata acgggatata tttctttata aatagatttt
     4381 aattcttcta tttcgtttcc ctttgccaga tcctttttgt taatgcagat aacgggcctc
     4441 acattatgct tttccgccat aacaagaaaa cgatccagta taaatgtgct gggtggtggt
     4501 tctgcagcag acaccatgac cgccagaacc tctatattgg ctatgggcgg tcttatgaaa
     4561 aaattgtctc ttgggaaaat cttttcaacc gtgccgtcac tgttttcatc tatacttatt
     4621 tcaactatat caccgaccaa agggacaact tctttttttt tgaatatacc ccttgctctg
     4681 cagcgataga tgtctccgtc acatttcaca taatagaatc cggctatgcc tttaataatg
     4741 attcccttca tccaaacgcc ccggtcaaaa gctcactttt ttggtcataa cagtttcatc
     4801 gtcaaatata actgttatgg tcccgctgcc ggtcccggat atattgacgg tttctccgcc
     4861 atcgctcttt tgtctctgct ctcccgaaat aactgttctt gttccgttgc tgtccgaaac
     4921 cgttactgtc agataaaaga tctcctgctt tgcttttgaa tagctcatat caactgcgat
     4981 ggttgaagtt c
//
LOCUS       DAONHK010000082         4882 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_13489_length_4882_cov_5.095919, whole genome shotgun sequence.
ACCESSION   DAONHK010000082 DAONHK010000000
VERSION     DAONHK010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4882
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13489_length_4882_cov_5.095919"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..334
                     /gene="hslV"
                     /locus_tag="PLM92_03995"
     CDS             <1..334
                     /gene="hslV"
                     /locus_tag="PLM92_03995"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015311653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HPR24334.1"
                     /translation="EKLEEHGGNLKRAAVALAQLWRTDKMMRNLEALMIVADKEAMLL
                     ISGNGEVIEPDQDFIAIGSGGNYAYAAANALFNHTEKNAEEIVKESLRIASSICVYTN
                     DNITVEKL"
     gene            346..1791
                     /gene="hslU"
                     /locus_tag="PLM92_04000"
     CDS             346..1791
                     /gene="hslU"
                     /locus_tag="PLM92_04000"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003869176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HPR24335.1"
                     /translation="MGNKLYYMTPKEIVAELDKYIIGQDEAKKSVAIALRNRYRRSLL
                     PEELMEEITPKNILMMGPTGCGKTEIARRLAKLMDAPFVKVEATKFTEVGYVGRDVDS
                     MIRDLVEASIRITKQNRLEEKYAIAEEMAEEKIIEAIIPGNKKNGKTQGAGGPFDFIL
                     NSGGYQSQKQQYEEQQAKKGDEENKSTVQDIEMAREQVRQQLKDGKLEEQYIEIQVND
                     VPKGNQLDMSQEGMSIAIGNIFGDMMPQKKKTKKVKVKDARKILREEEAQNLIDMDQV
                     TEEALENAEQNGIVFIDEIDKIASGSSYRSGADVSREGVQRDILPIVEGSVVNTKYGP
                     VKTDHVLFIGSGAFHTAKPTDLIPELQGRFPIRVELENLTEETLLKILTIPENALTKQ
                     HKALLETEGIKIDFSDDAIKEIARMAFLMNEQTENIGARRLHTILEKLLEDISFNIPE
                     MKEEKIVIDAEYVKEKFIEKIHPEDLDRYIL"
     gene            1904..2683
                     /gene="codY"
                     /locus_tag="PLM92_04005"
     CDS             1904..2683
                     /gene="codY"
                     /locus_tag="PLM92_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013361710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HPR24336.1"
                     /translation="MENEILTKVRKLNWVLQEAPKGKFSFGELCEILSNLSDANVYLV
                     NPRGKIIGVHYKIEADSSTIEDPETGIEKLPNEYTEAMMQIVKTEANLRGDEVLKIFK
                     YDYNTQEKYHTFIPIIGGEERLGTLVLARYSPEFSDEDLVLGEFGATVVGLEINRRKA
                     LENEEEERNEEIVKMAISTLSYSESEAVQKIFEELKNGEGILVASKIADRSRITRSVI
                     VNALRKLESAGIIESKSLGMKGTHIKVLNRKFYQQLDKLKN"
     gene            2741..4021
                     /gene="hflX"
                     /locus_tag="PLM92_04010"
     CDS             2741..4021
                     /gene="hflX"
                     /locus_tag="PLM92_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013361540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HPR24337.1"
                     /translation="MFRIDDENKIISSEECRAVIVGIQKNRDISYSMEELAGLAEAAG
                     AHVTGRMTQKTEKMNPATLIGAGKVLELAEICENMEVDTVIFNEELNGAQIRNLENAL
                     DRRVIDRTILILDIFAMRALSKEGRLQVELAQLKYRLPRLTGFGKSLSRLGGGIGTRG
                     PGEKKLETDRRHINKRINDIKKELVHIKTSREAQNSKRKRSRIPVVALVGYTNSGKSA
                     LMNKMLEMTEKEEKAVVSEDMLFASLDTQHRSINLKDGKTFILVDTVGFVSELPHMFL
                     DAFKATLEEAVYADLLLQVTDLTCQDQDFHNEVTKKVLEEIGAGHKEKITVYNKADMV
                     FEKERPASGSGSVAVSAETGENMETLMRMIQSRIFSDRIDVYLLIPYSNGELFSYICE
                     RGTVKSKEYLDEGIRVKAQIPQKDYHRVKKYEIV"
     gene            4008..4625
                     /locus_tag="PLM92_04015"
     CDS             4008..4625
                     /locus_tag="PLM92_04015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HPR24338.1"
                     /translation="MKLYKTVAGEASARQTIEKSRFIARIKPVSSKKEADDFIEGIRK
                     KNKDATHNVPAMVLGDKFQIQWASDDGEPQGTSGAPILYMLIREGITNVVVVVTRYFG
                     GIKLGTGGLVRAYTGTVKLAIERASVREISEMAEMTVKIDYTFLAGLKNAAEAEDFSI
                     ENIEYKEKITVVLIVPSEKKEKIKKLVAGITSGSAKVVSERLIKC"
     gene            4720..4795
                     /locus_tag="PLM92_04020"
     tRNA            4720..4795
                     /locus_tag="PLM92_04020"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4753..4755,aa:Val,seq:tac)
     gene            4804..4880
                     /locus_tag="PLM92_04025"
     tRNA            4804..4880
                     /locus_tag="PLM92_04025"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4838..4840,aa:Asp,seq:gtc)
BASE COUNT         1757 a          843 c         1193 g         1089 t
ORIGIN      
        1 agagaaactt gaagagcacg gaggtaacct taagcgcgca gctgtggccc tggctcagct
       61 gtggcgtacg gacaagatga tgagaaatct tgaggcactg atgatagttg cagataaaga
      121 agccatgctt ttgatatccg gaaacggaga agttatagaa ccggatcagg acttcattgc
      181 cataggttcg ggcggcaatt atgcctatgc ggcggcaaat gcgcttttca atcacactga
      241 aaaaaatgcc gaagaaatcg taaaagaatc attaaggata gcatcttcta tttgcgtata
      301 caccaatgat aatataacag tcgagaaact gtaaggagga cacagatggg aaacaaatta
      361 tattacatga ctcccaagga gatcgttgcg gaacttgaca aatatataat aggacaggat
      421 gaagcaaaaa aatctgtggc tatcgccctt aggaacagat atagaagaag tcttctgccg
      481 gaagaactta tggaagagat aactcccaag aatatactta tgatggggcc gaccggatgt
      541 ggtaaaactg aaatagcaag aagacttgcc aaactgatgg atgcaccctt tgtcaaagta
      601 gaagcgacca agtttacaga agtgggttat gttggccgtg atgttgattc catgataaga
      661 gatcttgttg aggcatccat aaggataaca aaacaaaacc ggcttgaaga aaaatatgcc
      721 attgcagaag aaatggcgga agaaaagatc atagaagcaa taatccccgg aaacaaaaaa
      781 aacggaaaga cccagggagc cggaggccct tttgatttca tattgaacag cggcggatac
      841 cagagccaga agcaacagta tgaagaacag caggcaaaaa agggagatga agaaaataaa
      901 agtacggttc aggacattga aatggccaga gagcaggtca ggcagcagct taaggacgga
      961 aaactggaag aacagtatat agaaatacag gtcaatgatg tgccgaaggg caatcagctc
     1021 gacatgagcc aggaaggcat gagtatagcc atcggcaata ttttcggaga catgatgccc
     1081 caaaagaaga aaacaaaaaa agtaaaggtc aaggatgcca gaaagatatt aagagaggaa
     1141 gaagcccaga accttataga catggatcag gtcactgaag aggccctcga aaacgctgaa
     1201 cagaacggca tagtatttat tgatgaaata gataagatag cctcgggttc aagttacaga
     1261 tccggtgcag atgtttccag agagggagta caaagagaca ttctgcccat agttgaaggc
     1321 agtgtggtaa acacaaaata cggtccggta aagacagatc atgttctttt cataggatcg
     1381 ggtgctttcc atacagcaaa gccaacggat cttattcctg aactgcaggg acgttttccc
     1441 ataagggtcg aattggagaa tctgacggaa gaaacccttc taaagatact taccatacct
     1501 gaaaacgccc ttacaaagca acataaagcc cttcttgaaa cagaaggaat aaaaatcgat
     1561 ttttccgatg atgcaataaa agagatcgca cgtatggctt ttcttatgaa tgaacagacg
     1621 gagaacatag gtgcaagaag acttcatacc atacttgaga agctgctgga agatatttca
     1681 ttcaacatcc ccgagatgaa ggaagaaaag atcgtaatag acgccgaata tgtaaaagag
     1741 aaattcattg aaaagataca tccggaagat ttggatcgtt acatactgta attgttgaaa
     1801 tgttataaaa attcaaaaaa atcagttgca acgactcctt ggagtatata taatatataa
     1861 gtggttaaaa atacttatat aaacgtaaca ggagtgtcat aaaatggaaa acgagatatt
     1921 aacaaaagta cgcaaactta actgggtact tcaggaagct cccaagggca agttttcatt
     1981 cggagaattg tgtgagatac taagcaatct ttcggatgca aatgtatacc ttgtaaatcc
     2041 cagaggcaag ataataggtg ttcattataa aatagaagcg gatagttcta ctatagaaga
     2101 tcctgaaacc ggaatagaga agctcccgaa tgaatataca gaagcaatga tgcagatcgt
     2161 aaagaccgaa gccaatctga ggggagatga agtactgaaa atattcaagt atgattataa
     2221 tactcaggaa aagtaccaca cattcattcc cataatagga ggagaagagc gattggggac
     2281 actggtcctt gcaaggtatt caccggaatt cagcgatgaa gatctggttc tgggagaatt
     2341 cggggcaact gtggtgggac ttgagatcaa cagaagaaag gctcttgaga atgaagaaga
     2401 agaaaggaat gaagaaatcg taaaaatggc cataagcacc ctttcatatt cggagagcga
     2461 agcggtgcag aagatttttg aggaacttaa aaacggagaa gggatacttg ttgccagcaa
     2521 gatcgcagat cgttccagaa taacaagatc agtcattgtc aatgctttga gaaaactgga
     2581 gagcgcagga ataatagagt caaaatctct gggcatgaag ggaactcata taaaagtact
     2641 caaccgtaaa ttctatcagc aactggataa attgaaaaat taaaagaaat aaatgctccg
     2701 gacttttgtc cggggcgttt ttttatgtta aaatcagtat atgttcagaa tagatgatga
     2761 aaacaaaata ataagcagtg aagagtgcag ggctgttata gtcggcatac agaaaaacag
     2821 ggatatatcc tattccatgg aagaactggc cggtctggca gaagccgccg gagcccatgt
     2881 tacaggccgg atgacacaga aaacagaaaa aatgaatccg gcaacactga taggagcagg
     2941 taaagtcctt gagttggcag aaatatgcga aaacatggaa gttgatactg tcattttcaa
     3001 tgaagaactt aacggagcac aaatacgcaa tctggagaat gccttggacc gaagagtcat
     3061 agacaggacc atacttattc ttgatatatt tgccatgcgg gctctttcca aggaaggccg
     3121 gcttcaggtg gaactggctc agctcaaata cagactgccc agattgaccg gattcggaaa
     3181 atctctttca aggctgggcg gaggaatagg aaccagggga cctggggaaa agaaactcga
     3241 aacagataga aggcatatca acaaaaggat aaatgatata aaaaaagaac tggtccatat
     3301 caaaacaagc agagaggctc agaattcaaa aagaaaaaga tcccggatcc cggtagttgc
     3361 ccttgtgggc tataccaact cggggaaatc ggcccttatg aacaaaatgc tggaaatgac
     3421 cgagaaggaa gaaaaggctg ttgtctcgga agatatgctt tttgccagtc ttgacacaca
     3481 gcacagaagc ataaatttaa aagacggcaa aacctttatt ctggtggaca cagtaggttt
     3541 tgtaagtgaa cttccccata tgtttcttga tgcattcaaa gctacattag aagaggcggt
     3601 gtatgcggat cttcttcttc aggtcacaga tctcacatgt caggatcagg attttcacaa
     3661 cgaagtaaca aaaaaagtgc tggaagagat aggagccggt cataaagaaa aaataactgt
     3721 gtataataaa gcagatatgg tttttgagaa ggaaagacct gcttcaggca gtggatctgt
     3781 tgccgtgtcc gcagagaccg gagaaaacat ggaaacactg atgagaatga tacaaagcag
     3841 gatattttct gacaggattg atgtttatct tctcatacca tacagtaatg gggagctctt
     3901 ttcatatata tgtgaaagag gaactgttaa aagcaaagaa tatttggatg aaggaataag
     3961 agtaaaagca cagataccgc aaaaagacta tcacagggtg aaaaaatatg aaattgtata
     4021 aaacagtcgc cggggaggca agcgcccggc agacaataga aaaatccaga tttatagcca
     4081 ggataaagcc ggtttcatca aaaaaagaag cagatgattt tattgaggga ataagaaaaa
     4141 agaataaaga tgcaactcac aatgtccctg ctatggttct gggagacaaa tttcagatac
     4201 agtgggcgag tgatgacgga gagccccagg ggacttcggg tgcgccaata ctttacatgc
     4261 tgatcaggga aggtataaca aatgttgttg ttgtggtcac cagatatttc ggagggataa
     4321 aactgggaac gggaggactt gtaagggcat atacggggac tgtaaaactt gctattgaaa
     4381 gggcctccgt acgggaaata tcggaaatgg cggaaatgac tgtaaaaatc gattatacat
     4441 tccttgccgg tcttaaaaat gctgccgaag ctgaagattt cagcattgag aacatagaat
     4501 ataaagaaaa aataacagtt gtgctgattg ttccttcaga gaagaaagaa aaaataaaaa
     4561 aacttgttgc gggcattact tcaggcagtg caaaagtggt gtccgaaagg ctcataaaat
     4621 gttgaaaaaa tagtattatt agtgccgaaa acacttgaca gtaaacagta gatactgtat
     4681 attatgtaag tgcgtcaagc agttttttgg acatgaggag ggcgcttagc tcagctggga
     4741 gagcacctgc cttacaagca gggggtcata ggttcgatcc ctatagcgcc caccataata
     4801 aatggtccgg tagttcagat ggttagaatg ccagcctgtc acgctggagg tcgtcggttc
     4861 gatcccgatc cggatcgcca at
//
LOCUS       DAONHK010000083         4836 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_13632_length_4836_cov_4.700899, whole genome shotgun sequence.
ACCESSION   DAONHK010000083 DAONHK010000000
VERSION     DAONHK010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4836
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13632_length_4836_cov_4.700899"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..793)
                     /locus_tag="PLM92_04030"
     CDS             complement(<1..793)
                     /locus_tag="PLM92_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010965061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HPR24339.1"
                     /translation="MAFEGLSEKLQGVFKGLKNKGSLTEADIDEAMREVKLALLEADV
                     NFKVVKEFVASVKEKALGAEVMKSLTPAQQVIKIVNDQLTDLMGGSGSKLTYSSSGFT
                     VIMMVGLQGTGKTTTCGKLALWLKKNGKKPMLCACDIYRPAAIDQLEIVGKSADTPVF
                     IMRDSKNPEEIAIKAKKEAELKGFNVLIVDTAGRLQIDEKLMEELDVIKKSVRPHEIL
                     LVVDALTGQDAVNAAEGFNSKLGIDGIIMTKMDGDSRGGAALSTKK"
     gene            complement(802..1158)
                     /locus_tag="PLM92_04035"
     CDS             complement(802..1158)
                     /locus_tag="PLM92_04035"
                     /inference="COORDINATES: protein motif:HMM:NF016205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxM family DNA-binding protein"
                     /protein_id="HPR24340.1"
                     /translation="MFDKITEISLLHDFYGQLLTDRQQQVMRLYHEENLSLSEIAHEF
                     GISRQGVHDTLKSAEKTLTGYEIKLGLVEKFKKTDGAVKKIDDIIDRIISENSIDKGS
                     KNKLNSIKKIIDEINE"
     gene            complement(1231..2259)
                     /gene="sppA"
                     /locus_tag="PLM92_04040"
     CDS             complement(1231..2259)
                     /gene="sppA"
                     /locus_tag="PLM92_04040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HPR24341.1"
                     /translation="MDKKSKMKKPLIILGSILGGLIVLGLIFAAIGGNPVNRAAGLFG
                     ADMSGGSQQTDYSYVSEDHIAVIHVDGIIGADTSGSFLSEPLGYDHQFTMDSIDEAIE
                     NEDNKGLIVFVDSPGGSVYETDELYMKIKEYKKKTERPVYAYMGSMAASGGYYLSAPA
                     DKIFASRNCWTGSIGVTVGTFYDISGLLEKYGIRTVTIDSGNNKSMGSMTEPMSSEEK
                     EIWQRLVDEAYEQFVDIVADGRGMSIDNVKKLADGRIYSASQAKKNGLIDEIGTFDDA
                     ANDMKKQNDLGPCEIYEVYPPEESLIDQLFMKISESKESGSDIERLLGIINENGNYPV
                     AYICEKLK"
     gene            complement(2283..2558)
                     /locus_tag="PLM92_04045"
     CDS             complement(2283..2558)
                     /locus_tag="PLM92_04045"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HPR24342.1"
                     /translation="MKEDNGKKWREGVYLCTCESSLKADIIESKLRSEGVPCLKKYKG
                     ASNFIEIAMGNTTAFPIDIYVREETLEDARNIIVAVPIDEDFKETEE"
     gene            complement(2555..4258)
                     /gene="thrS"
                     /locus_tag="PLM92_04050"
                     /pseudo
     CDS             complement(2555..4258)
                     /gene="thrS"
                     /locus_tag="PLM92_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100426.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
     gene            complement(4309..>4836)
                     /locus_tag="PLM92_04055"
     CDS             complement(4309..>4836)
                     /locus_tag="PLM92_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HPR24343.1"
                     /translation="GDYPFFVKPANAGSSVGISKVKNQIGLFEGITRAAAEDHKIIIE
                     EAISGRELEVSVLGNRDPKASAVGEILPANEFYDYEAKYINKKSRTRIVTDLSPQTEE
                     EIRAEAVSIYKTMNCRGMARVDFFLDEKNQIIFNEINTIPGFTSISMYPKLWEACGLS
                     YEKLIDRLIELAMEE"
BASE COUNT         1078 a         1190 c          849 g         1719 t
ORIGIN      
        1 ctttttttgt ggaaagcgct gcccctccac gggagtcgcc gtccatcttt gtcattatta
       61 taccgtcgat accaagtttg ctgttgaatc cttctgcagc attgactgca tcctgtcctg
      121 tcagcgcatc aactaccaga agtatttcgt ggggtcttac tgattttttt ataacatcca
      181 gctcttccat aagcttttca tctatctgca gtctgccggc tgtatctact ataagcacat
      241 tgaagccctt tagttccgct tcttttttcg cttttattgc tatttcctcg ggatttttgc
      301 tgtctctcat tataaaaacg ggtgtatctg ctgatttgcc cactatttcc agttgatcta
      361 tagccgcagg cctgtatata tcacatgcac acagcatggg ttttttgccg ttttttttca
      421 gccataaagc cagtttcccg catgttgttg tcttgcctgt tccctgaagc cccaccatca
      481 ttatcactgt gaatccggag gatgaatagg tcagcttgct gccggaaccg cccatcagat
      541 ccgtaagctg gtcattgact attttgatta cctgctgtgc gggggtaaga cttttcatga
      601 cttctgcccc cagagccttt tcttttaccg atgctacgaa ttcttttacc accttaaaat
      661 tcacatcggc ttcaagaagt gccagcttga cttccctcat tgcctcatct atatcagcct
      721 ctgttaagga acctttgttt tttagtccct tgaacacgcc ctgtagtttt tcgctcaatc
      781 cttcaaacgc catctcattc cctactcatt tatttcatct attattttct tgatgctgtt
      841 aagtttgttt tttgatcctt tatcaatact gttttcactt attatccggt ctattatgtc
      901 gtctattttc ttcactgctc cgtcggtttt tttgaatttc tccacaagcc caagtttgat
      961 ttcatatccg gtcagtgttt tttctgcact ttttaatgta tcatgaaccc cctgtctgct
     1021 tataccgaac tcatgggcta tttccgacag ggaaaggttt tcttcgtgat aaagcctcat
     1081 gacctgctgc tgtctgtccg taagaagctg tccgtaaaaa tcatgaagca ggcttatttc
     1141 cgttatttta tcaaacatac aatgtaattt aacacaaacc aaaggagctg tcaagtacta
     1201 tgccttgtca gctccttggt ttttttgttt ttacttcagt ttctcacaaa tatacgctac
     1261 aggataattc ccgttttcat tgattatccc aagaagtctt tcaatatcgc ttccgctttc
     1321 ttttgattct gatattttca tgaacagctg atcaataagg ctctcctcag gcggatagac
     1381 ttcatatatc tcgcatggcc ccagatcatt ttgttttttc atatcatttg cagcatcatc
     1441 aaaggttcct atttcatcta tcagtccgtt tttctttgcc tgggatgccg aatatatcct
     1501 tccgtctgca agttttttga cattgtcgat gctcatgcct ctgccatcag caacgatatc
     1561 cacaaactgt tcgtatgctt catcaacaag cctctgccat atttcctttt cttcactgct
     1621 catgggctca gtcatgctac ccatggattt gttgttgccg gaatcaatgg ttactgtccg
     1681 gataccgtat ttttcaagga gacccgagat gtcataaaaa gtaccgactg taacgcctat
     1741 tgaaccggtc cagcagtttc tgctggcaaa tatcttatcc gctggcgcag aaagataata
     1801 tccgccggaa gctgccattg atcccatata ggcataaacg ggcctctcag tctttttttt
     1861 gtattctttt atcttcatat aaagttcgtc tgtttcataa acggatcctc cgggcgaatc
     1921 aacaaaaact ataagccctt tgttgtcttc gttttctatg gcttcatcta tactatccat
     1981 ggtgaactga tgatcataac caagaggctc tgaaagaaaa ctgccggatg tgtcagcccc
     2041 aatgatcccg tcaacatgta tgactgctat atgatcctca gaaacatatg aataatcggt
     2101 ctgctgtgat cctccggaca tatcggcccc gaaaagtcct gcagccctgt ttactggatt
     2161 gcctcctatc gctgcaaaaa taagacccag aacaatcagt cctccaagta tagaaccgag
     2221 tattataagt ggttttttca tttttgattt tttatccata tgggttttcc tcttttatcc
     2281 tgtcactctt ctgtttcttt gaaatcttcg tctatgggca ctgctacgat gatattcctt
     2341 gcatcttcca gagtttcttc tcttacataa atgtcaattg gaaatgctgt agtattcccc
     2401 attgctatct ctatgaagtt gcttgcacct ttatatttct tgaggcaggg gacaccttca
     2461 ctccttagct tcgattctat gatatccgct ttcaaactgc tttcacaggt acagagataa
     2521 actccctcgc gccatttttt accgttatct tctttcatga agagaccgcc ttttcttcta
     2581 tttcttttag cagcatggct ttgaaccgct caagtggtat ttctccaagt tcgcctcttt
     2641 ttccggaacg gacagaaagg ctttttgctt cttcttctct ttcccctatt acgcctatgt
     2701 atgaaactct ttcatttctt gcttcacgca ttttatatcc tattttttca tttcttatat
     2761 ccagttcgac ccgcagtcct gcttttctga gttcttctgc tgtttgttcc gcatactctg
     2821 aaaacttttc tgtaacagtg agcagtttaa cctgaacagg ggccagccaa aggggaaact
     2881 tgccggcgaa gtgttctgtc agtatggcta tgaatctttc gatggaaccg aaaataactc
     2941 tgtgtatcat tgccggcctg tgtttctctc cgtccgctcc aatatagttt gaatcaaacc
     3001 tctggggcat ctggaaatca agctgtatag tgccgcactg ccatgtcctg cctatggagt
     3061 cctccagatg aaaatcaagt ttgggcccgt aaaaagcccc gtcaccttcg ttcactgtat
     3121 actccattcc tatttcctct gttgcttctc ttaaagcttc agtggctctt tgccattctt
     3181 catcagtacc catggcatct tccggctttg tggagagctc aatgtgatat ttgaatccaa
     3241 aaaggctgta aacataatct ataaatccta tcacgccctt gatctcatcc tttatttgtt
     3301 ccggtaacat gaatatatgt gcatcatcct gagtaaatgt ccttaccctc ataagaccgt
     3361 gaagtgctcc cgaaagctca tggcggtgca cctgtcccag ctctcccatt cgtattggca
     3421 ggtctctgta tgaatgtatc tttcttccgt aaaccatcat tgctcccggg cagttcatag
     3481 gcttcacggc aaaatccaca ttatctattt tggtaaaata catattgtct ttgtaatgat
     3541 cccagtgtcc cgaggtatgc cagagctcct cctggagtat aagcggagtc tttatttctt
     3601 catatcctct tttttcatgt tcctgcctcc agaatttttc cagctcgttc cttattacca
     3661 tgcctttagg cagaaagaaa gggaatcccg gaccttcgtc ataaagggca aaaaggtcca
     3721 tttcctttcc tatcttcctg tgatcacgtt tttttgcttc ctcaattctt ttcagatatt
     3781 gatccagctc ttcctggctt ggaaaggcag ttccgtatat tctctggagc atctttttat
     3841 ccgagtcgcc tctccagtag gctcctgcca cactggtcag atttattgcc tttatctggc
     3901 ttgttttttc catatgaggt cctgcacaaa gatcaacgaa atctccctgt ttataaaacg
     3961 aaagtttttc atcctcggga agatcctgaa taagttccac tttgtaatct tccttccgct
     4021 ctttcatcag ctttattgcc tcttctctgg gaagctcaaa tctttcgagt ggcagaccgg
     4081 actgtattat tcttttcatt tcctttgtta tttttttgag atcttcatca ttgaatttat
     4141 aatcaagatc gaagtcataa taaaacccgt tttctatggc aggtccgatt gccagttttg
     4201 cctccggcca gatttttttt acggcatgag ccattatatg cgaggcagta tgcctgtatt
     4261 tttttctcac ttcttcattt gagaagtcta tattttcttt tgccattttt attcctccat
     4321 tgccagttcg atcagcctgt ctataagctt ttcatagctt agtccgcaag cttcccaaag
     4381 cttgggatac atgcttatac ttgtgaatcc cgggatcgtg tttatttcgt tgaatatgat
     4441 ttgatttttt tcatcaagga aaaaatccac ccgggccatt cctctgcagt tcattgtttt
     4501 atatatagaa acagcctctg ctcttatttc ttcttctgtt tgcggactca gatctgttac
     4561 gatgcgcgtc cgggattttt tgtttatata ttttgcttca taatcataaa actcatttgc
     4621 cggaagtatt tctcccactg ccgaagcctt tggatctctg ttgcccaaaa cagaaacctc
     4681 cagttctctt ccggatatag cttcctctat tattatcttg tggtcttcgg cggcggctct
     4741 ggttattccc tcaaaaagcc cgatctggtt tttgactttg gatattccca cagaagatcc
     4801 tgcatttgcc ggtttcacga aaaaggggta atctcc
//
LOCUS       DAONHK010000084         4792 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_13819_length_4792_cov_3.957568, whole genome shotgun sequence.
ACCESSION   DAONHK010000084 DAONHK010000000
VERSION     DAONHK010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4792
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13819_length_4792_cov_3.957568"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1427
                     /locus_tag="PLM92_04060"
     CDS             <1..1427
                     /locus_tag="PLM92_04060"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005837953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HPR24344.1"
                     /translation="ELAISISNLAVDKLHIIGDIFDRGAHPDDIMDFLMDYHNVDFQW
                     GNHDIVWMGAATGNWACISNVLRMNISYNNFDMLEIGYGINLRPLAVFAERVYGDDPC
                     EHFKPKMLDKNKFDPVDEDLAAKMHKAIAICQFKVEGQRVMAHPEYELENRLLLDKID
                     LKNGTVKINDKVYELRDKNLPTIDPENPYELTNLEKRLMRILEASFLKSDKLQEHIKF
                     LYSHGSLYKIVNGNLLYHGCIPMNREGEFKECTVNEVTKKGREYIDYLDDEIRKAYYS
                     PNKSGETGRSGDLMWYLWLGKSSPLFGKDQMTTFERLFIDDKSTHKEKTVEYYSLIKE
                     RKTCEKILREFGLDPEKSKILNGHVPVKIKDGESPVKGEGLLYIIDGGISKAYQKTTG
                     IAGYTFIFNSWFMALSEHKPYQPLQSDGTQKFFTPTIKTVDMLKERMYIKDTDDGKKL
                     MGEIKELEALIEAYKDGEIKEDNE"
     gene            complement(1534..2217)
                     /locus_tag="PLM92_04065"
     CDS             complement(1534..2217)
                     /locus_tag="PLM92_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009644209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPR24345.1"
                     /translation="MDQEKIGSFLKKLRKEKRLTQEQLAEEFNVAGRTVSRWETGNNM
                     PDLSLLVKIADYYDVDIREVINGERKSKKMDNEIRDTLVKVAGYGDVVKKSETGKLHQ
                     FIGAILAGFGAFIIISSFAVFPSDSSWGSIYSVIGCVIASIGIYKLCAVIKITKKPQR
                     IICTIGCFLLMLCCLHGLDYMSVKAYKQPPRFCYEKSYGEDVIEYKTLFYTVTRYNVD
                     ADDEYYEFN"
     gene            complement(2601..2677)
                     /locus_tag="PLM92_04070"
     tRNA            complement(2601..2677)
                     /locus_tag="PLM92_04070"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2641..2643),aa:Arg,seq:acg)
     gene            3151..3753
                     /locus_tag="PLM92_04075"
     CDS             3151..3753
                     /locus_tag="PLM92_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005363495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HPR24346.1"
                     /translation="MVQERMKQILENEAVEIRWHGRGGQGAKTACLLLAEVAFNSGKH
                     VQGFPEYGPERMGAPITAYNRITKEHCKVHSNIYHPDYVVVVDETLLHSVDVARGIKD
                     GGALIINTGKDREEIRPLLNGYEGNIYTLDAERISMETMGKNFPNTPMLAAAVKVSNL
                     MDEESFVKDMEESFRHKFAHKPHLIEGNMAALKMSLKEVQ"
     gene            3755..4075
                     /locus_tag="PLM92_04080"
     CDS             3755..4075
                     /locus_tag="PLM92_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016323754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPR24347.1"
                     /translation="MNKTAKKSAAEINEKITWQEITTGAEICEGGTSKLVNTGDWRVK
                     VPVFIEEKCKHCMLCVPFCPDSSIPVKDGRRLDFDLMHCKGCGVCVKVCPFGAIDFVK
                     EEKK"
     gene            4072..>4792
                     /gene="porA"
                     /locus_tag="PLM92_04085"
     CDS             4072..>4792
                     /gene="porA"
                     /locus_tag="PLM92_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015907412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate ferredoxin oxidoreductase"
                     /protein_id="HPR24348.1"
                     /translation="MSIRERMSGNEAVAHAMKQIEPDVMGAFPITPSTEIPQYFSDYI
                     ANGKVDTEFVAVESEHSAMSVCMGASAAGARAVTATSSAGLAYMWELLYVVASSRLPV
                     TMAVVNRALTGPININNDHSDSMGARDSGWIQIYAETNQEVYDNFIQGVIIAEKSRLP
                     VMICQDGFITSHGVSNIELIEDEKVKEFVGDYRPENYLLKKENPLAVGPYGVSPYYME
                     VKRQQAQAMKDSMPVIKKTAEE"
BASE COUNT         1579 a          922 c         1082 g         1209 t
ORIGIN      
        1 aagagctggc catatcaata agcaatcttg ctgtggacaa gcttcatata ataggagata
       61 tatttgacag aggcgctcac ccggatgaca taatggattt cctgatggat taccacaatg
      121 tggattttca gtgggggaat catgatattg tctggatggg agcggcaacg ggaaactggg
      181 catgtatatc aaatgtcctt agaatgaata taagctataa taatttcgat atgctggaga
      241 tcgggtacgg aataaatctc cgccctctgg cggtgttcgc agaaagagta tacggcgatg
      301 atccctgtga gcatttcaag ccaaaaatgc tggacaaaaa caagttcgat ccggtggatg
      361 aagacctggc ggcaaagatg cacaaggcta tagccatatg ccagttcaag gtggagggtc
      421 agagagtaat ggcccatccg gaatacgaac tggagaacag acttctactg gataaaatag
      481 acctcaaaaa tggaacggta aaaatcaatg ataaggtata tgaactgcgg gacaagaatc
      541 ttcccaccat cgacccggaa aacccttatg aactgacgaa tttagagaaa cggttgatga
      601 ggatactgga agcatctttt ttgaaaagtg ataaacttca ggaacacata aaattcctct
      661 attcccatgg atctctttat aaaatagtaa acggcaatct cctttatcat ggctgcatac
      721 ccatgaaccg ggaaggcgag ttcaaggaat gtactgtaaa tgaagtaacg aaaaaaggca
      781 gggaatatat tgattacctg gatgacgaaa taagaaaggc atattacagt cccaacaagt
      841 caggggagac cggcagatca ggagatttga tgtggtatct gtggctgggc aaaagctcgc
      901 cgcttttcgg aaaagatcag atgaccacat ttgaaagact tttcatcgat gacaaatcga
      961 cacacaaaga aaagacggta gaatattatt ctcttataaa agagagaaaa acatgtgaaa
     1021 agatattaag agaattcggt ctggaccccg aaaaaagcaa gatacttaac ggacatgttc
     1081 cggtcaaaat aaaagacggg gaaagcccgg tcaagggaga agggctgctt tatatcatag
     1141 acggcggcat atcgaaagcc taccagaaga ctacgggaat agcaggatat acttttatct
     1201 tcaactcatg gttcatggcc ttgtccgagc ataagccata ccagccgctg cagtccgacg
     1261 ggactcagaa attcttcact ccgaccataa agaccgtgga catgctgaaa gagagaatgt
     1321 atataaaaga tacagatgac ggcaaaaaac tcatgggcga aataaaggaa ctggaagctc
     1381 tgatcgaagc ctataaggat ggcgaaatca aggaagacaa cgaatgatat tctgatgaaa
     1441 gctgttgtca ataaagacac aataaaaccg gaggaaatac ctccgttttt attttcctct
     1501 caatactgat tccgaatcat ttgtcaactt taatcaatta aattcataat attcatcatc
     1561 ggcatcgaca ttataccttg taacagtata aaacaaagtc ttatattcaa tgacgtcttc
     1621 gccatagctt ttttcatagc agaatcttgg tggttgctta taagctttga cgctcatgta
     1681 atcaagacca tgcagacagc atagcataag aaggaagcaa cctattgtac aaattatcct
     1741 ttggggcttt ttcgttattt tgattactgc acaaagtttg tatatcccga tagaagcaat
     1801 aacacaacca attacggaat agatagatcc ccagctgctg tcactgggga aaacagcaaa
     1861 tgaactaata ataataaacg ccccaaaacc agccaggatg gctcctataa attgatgtaa
     1921 cttgccggtt tcagattttt ttactacatc accataacct gcaactttaa ctaatgtatc
     1981 tctgatttca ttatccattt tcttactttt cctttcgcca ttaatgactt cacggatgtc
     2041 aacgtcgtaa taatccgcta tcttgacgag caaactaaga tcaggcatat tatttcctgt
     2101 ttcccatctg gaaactgttc tgccggcaac attgaattct tcagctaact gttcctgggt
     2161 caatcttttt tcttttcgaa gttttttaag aaaactcccg attttttcct gatccatttt
     2221 tctacctcct ttcatcaaaa tggtattgca tatttacgac catctacacg acacaaagtg
     2281 agaaatgccg ttaatgatca actggacaca tcttgtccgg ttgttttcct tctgtaattc
     2341 atgaatttat gcaatagtcc aatgaggtat aaattcaaat ttgttcattt ttgttgtata
     2401 ttagcaatta tatcacttct tcaatgtttg atataagcaa aatcccttta ttatcccttt
     2461 ggtaattgaa taaggcttga ttttactcaa atttaccaga ttatgtaaaa gatattagat
     2521 gatattggaa gataatataa ccttgaaaat cattgaaatt tcaaagaaaa aacccctaac
     2581 tattgcagtc ttggggtttt tggtgcgcca tgagggattc gaacccccga ccttcgcatt
     2641 cgtagtgcgc gactctatcc agctgagcta atggcgcagg cttactaaaa gcaccgttat
     2701 aaattataca gcaaggtatg gtttgttgtc aaaggaaata ataatcaata atggcagcaa
     2761 aaccgtcaaa taatgagaat aaacagcaat atttgttttg aaaaacaaaa aaaatacaaa
     2821 ttacttgttg acaacacatc aagaataata tagtatactg atgataattg aatatcaaag
     2881 actatgacgg agacagtagg gtcttttgaa agaacaaagc gaaccgggga tggtgagagc
     2941 ccggtatccg gtaaaaagac acacgaagat cacttctgag ttgcaggact gaagaaagta
     3001 agttctgacg gcttcccccg ttacagggaa cacgtatgat agtatgttgt atttggtggt
     3061 tagcgaacag cgcgagctgt cgcccttata caaggcggca gctttttgat tttgtacatc
     3121 aggacggcat aaaaatgccg ggaggataaa atggttcagg aaagaatgaa acagatactg
     3181 gaaaacgagg cggtggagat ccgctggcac ggtcgtggag gacagggcgc aaaaaccgcg
     3241 tgccttcttc tggcggaagt ggcatttaat tcgggaaaac atgttcaggg attccccgaa
     3301 tacggaccgg aaagaatggg agcacccata acagcatata accgtatcac aaaagagcac
     3361 tgtaaggtcc attcaaatat atatcacccg gattatgttg tggtggtgga tgagactctg
     3421 cttcattctg tggacgtggc aagaggcata aaagacggag gggcccttat aataaatacc
     3481 ggaaaagacc gggaagaaat aaggcctttg ctgaacggat atgaaggcaa tatatatact
     3541 ctggatgcag aacgcatatc gatggagacc atgggaaaga atttccccaa cacacccatg
     3601 ctggcggctg cagtaaaggt aagcaatctt atggacgaag agagtttcgt aaaggacatg
     3661 gaagaatctt tcaggcataa atttgctcat aaaccccatc ttatagaagg aaatatggcg
     3721 gctctgaaga tgtcgctgaa agaggtgcag taaattgaac aaaacagcga aaaaaagcgc
     3781 agcggaaata aacgaaaaaa taacctggca ggaaataacc acgggagccg aaatttgtga
     3841 aggcggcact tcaaaactgg tcaacacagg agactggaga gtaaaggtgc cggtatttat
     3901 tgaggaaaaa tgcaaacact gcatgctttg tgtgcctttt tgtcctgaca gttccatacc
     3961 ggtcaaagat ggcaggagac ttgacttcga tctgatgcac tgcaaaggtt gtggtgtatg
     4021 tgtcaaggtt tgtcccttcg gtgctataga ctttgttaag gaggaaaaaa aatgagtata
     4081 agagaaagga tgtccgggaa cgaagcggtt gctcatgcaa tgaaacaaat agagccggac
     4141 gtgatggggg cattcccaat tacaccttca acagagatac cccagtattt ctctgactat
     4201 atagcaaacg gaaaagtgga taccgaattt gttgccgttg aatccgaaca cagtgccatg
     4261 tcggtatgca tgggagcctc ggccgcggga gccagagccg ttactgcaac atcttctgca
     4321 ggacttgcat acatgtggga gcttctttat gtggtggctt catcaagact gccggtcacc
     4381 atggcagtgg tgaacagagc gctgacggga cctatcaata taaacaatga tcattccgat
     4441 tccatgggag ccagagattc gggatggata cagatatatg ccgagaccaa tcaagaagta
     4501 tatgataatt tcatacaggg agttatcata gccgaaaaaa gcagactgcc tgtgatgata
     4561 tgtcaggacg gattcataac cagtcatggt gtctccaaca tagagctgat agaagacgaa
     4621 aaagtcaaag agtttgtcgg tgactaccgg ccggagaatt atctgctcaa gaaagaaaat
     4681 cctcttgcag tgggacctta cggagtatcg ccctattata tggaagtcaa gagacagcag
     4741 gcgcaggcca tgaaagactc catgcctgta atcaaaaaga ccgcggaaga at
//
LOCUS       DAONHK010000085         4773 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_13891_length_4773_cov_4.157694, whole genome shotgun sequence.
ACCESSION   DAONHK010000085 DAONHK010000000
VERSION     DAONHK010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4773
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13891_length_4773_cov_4.157694"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1584)
                     /locus_tag="PLM92_04090"
     CDS             complement(<1..1584)
                     /locus_tag="PLM92_04090"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPR24349.1"
                     /translation="MTERKLGVFGIAFLYAGAIMGAGFASGREIWQFFGVFGKYGFAG
                     LAFVGALFIFVGIMTAIIARTLKTNDLGRIIVPGNSRILKAFTGYFMSFMLFTVVITM
                     TAAGGALFMQQFGASRILGGALIVILVIATVLGGFKRVAKVFGYVIPLLIIVMVIVCG
                     GVILKGIPESGIESQMIPSPLASVWQLAAVLYLSYNILAVVPIVANASINAKSTDHAV
                     LGAALGGSFLAVLALMITLALQTDAGFSESMDMPMLAFAMELSPVTGVIYTGVMLFAI
                     YSSATGNYYGFTTRLKNDGKKNRKTVIIAMAGFLLGLMGFRNVVAYMLPLEGFAGIAI
                     IIMLTCNFFIVMKNKKKEEKTYMFRDFHDHNRFDFPDGLVRVTAGFGGESILFFGSEK
                     TALFDCGMAYCGEELVQNIKEAIKRQEKKDGSARTLDYVMLSHTHYDHAGGIPYLRRC
                     WPELIVFGAAHGKAVFERPGAIKTIRTLGIAAEKYYGQGDPQKIIIEGLYIDKVIEEG
                     DNISLGKEYITVLETKGHTD"
     gene            complement(1577..2491)
                     /gene="rarD"
                     /locus_tag="PLM92_04095"
     CDS             complement(1577..2491)
                     /gene="rarD"
                     /locus_tag="PLM92_04095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HPR24350.1"
                     /translation="MSESRKQYKIGITAALICAVLWGVLPIYWQALRPIDSSVIIFYR
                     IMLVGVVCFIAALKLYGIKAIKAPLKKRGAKLKFFAAGLVITANWSIYIWAVNADFVI
                     QTCIGYYIEPLMVCIFGIIIFKEVLTKFKLTAFCFACAGVLLILLYFREIPLIALSLA
                     VTFAVYAAMKKTFNIEAMLSLFYETMFLVPVAAAVIVYLEVNGKGAIAVAEPYQYALL
                     FLSGILTAIPLGTFAVAANRVPLVTLGLIEYISPSITLIIGIFLFKEPFDLVQFMAFV
                     IIWIGLAIFTAGEIREANEIKRQGDIND"
     gene            complement(2512..3060)
                     /locus_tag="PLM92_04100"
     CDS             complement(2512..3060)
                     /locus_tag="PLM92_04100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPR24351.1"
                     /translation="MKEGEYMDLQDLLSFLEIDAPIEFEYFENFADLVECEETIPEST
                     LFSLFSETDKKIVGEIIVNYFDDMLDALPDDANDIYTLLEKIKLSMAGFIKASDDEDN
                     LARFSEQLAGFKTWYCFESAVGCKDPATGEEKLLTLRDALTLARLEKLEGEEYIYDFS
                     ECMNYEVDDYMVNISELTDPGR"
     gene            complement(3068..3883)
                     /gene="murI"
                     /locus_tag="PLM92_04105"
     CDS             complement(3068..3883)
                     /gene="murI"
                     /locus_tag="PLM92_04105"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006000558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HPR24352.1"
                     /translation="MDNRPIGFFDSGLGGLTCVPHLMKKLPNERIIYFGDTARTPYGS
                     KATSTIRKFSLQIADFLVENNVKMIVIACNTVSATCLYDLRERFPGTPVLGIIQPAAG
                     AVAENCSPSNRIGIIGTKVTIASHTYEDTILKQNGKLDIFESACPAFVPLIEEGIIEN
                     DIMDLTIRHYMDDFILENRIDTVVLGCTHYPLIRSNIERRYPDIRIIDPSEEIVSSIK
                     STLKENQMLAERPEKENTFYASDLSENFVRMIERIFMHSDVNVEFKNLDLEEA"
     gene            3962..>4773
                     /locus_tag="PLM92_04110"
     CDS             3962..>4773
                     /locus_tag="PLM92_04110"
                     /inference="COORDINATES: protein motif:HMM:NF020028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HPR24353.1"
                     /translation="MVKKLKNRKDIDKKYKWDISSMYPNERQWEKDVEKALKNAESFT
                     GFAGRLTKDSSTLLDAFKKRDSIWQTMEKAYVYARMKRDEDNEDRKYQSMCGKAGAYM
                     AKISAFCSFFVPELLKAPKDKLMAFIEEEPGLSVYRFAVTDILRQKKHILSKSEENIM
                     AQIGEVMPATNEIFTMLNNADLEFGNIKDENGRKVQLTHGNYINFMESRDRNVRRAAY
                     KKMYNCYREMINTIGAAYNYNTKTDVVNSRIRKYSSSRAAALSGDNIPDAVY"
BASE COUNT         1385 a         1107 c          936 g         1345 t
ORIGIN      
        1 gtctgtgtgg ccctttgtct caagtactgt tatatactct ttacccaggc ttatattgtc
       61 accttcttct atcactttat ctatatagag tccttcaatg attatttttt gaggatctcc
      121 ctggccgtaa tatttttctg cagctatacc cagagtcctg atcgttttta tggctcccgg
      181 cctttcgaaa acagccttgc cgtgggccgc accaaatacg atgagctccg gccagcagcg
      241 cctcaaataa ggaatacctc ctgcatgatc ataatgggtg tggctaagca tcacataatc
      301 gagagtccgg gccgatccgt cctttttttc ctgccgcttt atggcttctt ttatgttttg
      361 caccagttct tctccgcagt atgccatccc gcagtcaaaa agtgctgttt tttcacttcc
      421 gaaaaacagg atcgattctc ccccgaaacc tgctgttacc ctgacgagac catcgggaaa
      481 atcaaatctg ttatgatcgt gaaaatccct gaacatatat gttttttctt ctttcttctt
      541 gtttttcatg actataaaaa aattacatgt gagcattatt attattgcga tccctgcgaa
      601 gccttccagt ggaagcatat atgccacaac attcctgaat cccataagtc ccagaaggaa
      661 acctgccatt gctataatga ctgttttccg attttttttg ccgtcatttt taagtctggt
      721 ggtaaatcca tagtaattcc ctgtggccga agagtaaata gcaaaaagca ttacgcccgt
      781 atatatcaca ccggtaacag gggaaagttc catggcaaaa gccagcatag gcatgtccat
      841 tgattcggaa aaacccgcat cggtctgaag ggccagggtg atcataagcg ccagtaccgc
      901 caggaacgat cctcccagcg ccgctcccaa aacagcatga tcggtgcttt ttgcatttat
      961 tgatgcattg gcaacaatgg gcactacagc cagtatgtta tatgagagat aaagcactgc
     1021 cgccagttgc cagacggatg caagcggact ggggatcatc tgactctcta tgccgctttc
     1081 cggtatgcct tttaatataa caccgccgca gacaattacc attacaatta tgagcagcgg
     1141 aatgacataa ccgaaaacct ttgcgacccg cttaaacccg ccaagtactg ttgcaatgac
     1201 aagtattact atgagggctc ctcccagtat cctgcttgct ccaaactgct gcatgaacag
     1261 tgctcctccg gcagctgtca tggttatcac aacagtaaac agcatgaatg acataaaata
     1321 acctgtaaaa gctttcagta tcctgctgtt tcccgggact attattctgc ccagatcgtt
     1381 tgtttttagt gttcttgcta ttattgcggt cattatgccg acaaaaataa aaagtgcacc
     1441 cacaaaggca agacccgcaa atccgtattt accgaaaacg ccgaaaaact gccatatttc
     1501 ccggcccgaa gcgaagcctg ctcccatgat ggctcccgca taaagaaagg cgataccaaa
     1561 aacacccagt tttctttcag tcatttatat caccttgtct tttgatttca ttggcttccc
     1621 ttatttctcc tgcggtaaag attgccagcc cgatccatat tataacaaat gccatgaact
     1681 gaaccaggtc gaagggttct ttgaaaagaa atatacctat tataagggtt attgaaggcg
     1741 atatatactc aataagtccc agtgtaacaa gggggaccct gtttgcggcc actgcgaagg
     1801 tgccaagggg tattgctgtc agaatgcccg aaagaaaaag aagagcatac tgataaggct
     1861 ctgcaacggc aatggcccct tttccgttta cttcaagata aacgatcacc gccgccgcca
     1921 caggcacaag aaacatggtt tcataaaaaa gtgaaagcat ggcctctata ttgaatgttt
     1981 ttttcattgc agcatataca gcaaaagtca ccgcaaggct gagtgcgatg agagggatct
     2041 ctctgaaata caaaaggatc aaaagcaccc ccgcgcatgc gaagcagaat gcggtaagct
     2101 tgaattttgt cagtacctct ttgaaaatga taatgccgaa tatgcagacc atcaatggtt
     2161 ctatataata gcctatacat gtctgtatta caaaatcagc atttacagcc catatatata
     2221 tgctccagtt tgccgttatt acaagaccgg ctgcaaaaaa tttgagtttt gctccccttt
     2281 tttttagcgg agctttgatg gcttttatcc cgtaaagctt aagcgctgca ataaagcaaa
     2341 caacccccac aagcattatt ctgtaaaaga ttatcaccga agaatctatc ggccgaagtg
     2401 cctgccagta tatcggcagg accccccaca gtacggcgca tataagggct gctgttattc
     2461 ctattttata ttgtttcctg ctctcactca agatgtcctt tttcctttct tttaacggcc
     2521 cgggtccgta agttctgaaa tatttaccat atagtcatcc acctcataat tcatacattc
     2581 cgaaaaatca taaatgtatt cttccccttc aagcttttca agcctggcca gtgtaagtgc
     2641 atccctcaat gtgagaagct tttcttctcc ggtagcggga tctttgcagc ctacagccga
     2701 ttcaaaacag taccaggttt tgaagccggc caactgttct gaaaatcttg ccaggttgtc
     2761 ttcatcatct gaagccttga tgaacccggc cattgacagt tttatttttt caagaagagt
     2821 ataaatatca tttgcgtcat ccggcaaagc atcaagcata tcatcgaaat agttgacgat
     2881 tatttcaccc actatttttt tgtctgtctc ggaaaaaagt gaaaaaagag tgctttcggg
     2941 tatagtttct tcacattcca caaggtctgc aaaattctcg aaatactcaa attcgatggg
     3001 tgcatctatt tccagaaaag acagaagatc ctgcagatcc atatactcac cttctttcat
     3061 acatgagcta agcttcttcc aggtccagat ttttgaattc aacattgaca tccgaatgca
     3121 tgaatatacg ctcgatcatc cttacaaaat tctccgacag atcgctggca tagaaagtgt
     3181 tttctttttc cggacgttct gccagcattt gattttcttt aagggtgctt tttatactgc
     3241 ttacgatctc ttccgatgga tctattatcc ttatatccgg atatctcctt tctatgttgc
     3301 ttctgatcag tggataatgt gtgcagccca gaacgactgt atcaatgcgg ttttccaaaa
     3361 taaaatcatc catataatgt cttatggtca gatccattat atcgttttct attattccct
     3421 cttctataag aggcacaaat gcgggacatg cactttcaaa tatgtccagt ttcccgtttt
     3481 gtttaagtat cgtatcttca tatgtgtgac ttgcaatggt cacctttgta cctattattc
     3541 ctattctgtt gctgggagaa cagttttcgg ccactgcacc tgccgccggc tgtattatcc
     3601 ccagtacggg agtaccggga aatctttctc taaggtcata aaggcatgtg gcacttacgg
     3661 tgttacatgc aataacaatc atcttcacgt tgttttcaac aagaaagtcc gctatctgca
     3721 acgagaattt tcttatggtt gatgttgcct ttgacccgta aggtgtgcgg gccgtgtcgc
     3781 caaaataaat tattctttcg ttaggcaatt ttttcatcag atgaggcaca cacgtaaggc
     3841 cgccgagacc ggaatcaaaa aaacctatgg gcctgttgtc cattgtttat tatcctttca
     3901 agttggtgta taatatatat tacatcaaga atattgtaaa gtaaacacca cggaggatgc
     3961 aatggtcaaa aaactaaaaa acagaaaaga tatagataaa aaatacaaat gggatatcag
     4021 ctccatgtat cccaacgaga gacaatggga aaaagacgtg gaaaaagctc ttaaaaatgc
     4081 tgaaagtttt acaggatttg cagggcggct gacaaaagac agcagcacac tgcttgatgc
     4141 cttcaaaaaa agagacagca tttggcagac catggaaaaa gcatatgtct atgcaagaat
     4201 gaaaagagat gaggataacg aagaccgcaa gtaccagtcc atgtgcggca aagccggtgc
     4261 atatatggca aaaataagtg ctttctgttc tttcttcgtg cccgaacttc taaaggcgcc
     4321 aaaagataaa ctgatggctt tcattgagga ggagccgggt ctttccgtat acagatttgc
     4381 cgtgaccgac atccttcgcc agaaaaaaca tattctctcc aaatccgaag aaaacatcat
     4441 ggctcagatc ggtgaagtga tgccggctac aaatgagata ttcacaatgc tcaataatgc
     4501 agaccttgaa ttcggtaaca taaaagatga aaacggcaga aaagtccagc tgactcacgg
     4561 caattacatc aattttatgg aaagcagaga ccgcaatgta aggcgtgccg cctacaaaaa
     4621 aatgtacaac tgctacagag aaatgatcaa caccatcggt gcagcctata attacaacac
     4681 aaaaaccgat gtagtgaact cccgcatcag gaaatacagc tcctcaaggg cagcggctct
     4741 ttccggcgat aatatccctg atgcagtata tga
//
LOCUS       DAONHK010000086         4710 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_14127_length_4710_cov_4.098604, whole genome shotgun sequence.
ACCESSION   DAONHK010000086 DAONHK010000000
VERSION     DAONHK010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4710
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14127_length_4710_cov_4.098604"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1106)
                     /locus_tag="PLM92_04115"
     CDS             complement(<1..1106)
                     /locus_tag="PLM92_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006311832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HPR24354.1"
                     /translation="MNALAIIVPVAAIIGLIVAYGLTAWIGRADEGTDRMKEISGFIR
                     EGAMAFLSREYKTMVAVILLLFLIIGFAISWITAALYVVGACLSVLAGFFGMKVATKG
                     NVRTANAAMESGMSKALKIAFRSGAVMGLCVTGLGLLGLGVIFVIMGLDCVSVITGFG
                     LGASSMALFGRVGGGIYTKAADVGADLVGKVEAGIPEDDPRNPAVIADNVGDNVGDVA
                     GMGSDLFESYVGSTISAITLAAVAVANSGGLFDETAAALFPLLLSSVGIIASIIGILM
                     VRGKEGGNPATALNMGTYISGIIVIIFSFVLSYFMLGSIKYAVAIIGGLIVGIAIGKI
                     TEVYTSSDYKSVKKIAGQSETGPATTIIAGFGVG"
     gene            complement(1202..1453)
                     /gene="secG"
                     /locus_tag="PLM92_04120"
     CDS             complement(1202..1453)
                     /gene="secG"
                     /locus_tag="PLM92_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005837981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HPR24355.1"
                     /translation="MATLMIVFKVILAVASVILMISILMQSSNSAGLAGAIGGGAEQL
                     FGKKKSRGYEAILGRIATTAAVIFIVLSLVIVTLESHTA"
     gene            complement(1547..2293)
                     /gene="tpiA"
                     /locus_tag="PLM92_04125"
     CDS             complement(1547..2293)
                     /gene="tpiA"
                     /locus_tag="PLM92_04125"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009529735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HPR24356.1"
                     /translation="MRIPFIAGNWKMFKSRIETVVFAREFKELYKDTDVRTAICAPYV
                     HLDTLKAVFEGTQIKVGAQNVHFEDQGAFTGEVSALMLEEIGVDYCIVGHSERRQYFA
                     ETDETVNKKLHKLFETSIVPIMCVGEVLEQREAGTEAEVVKKQLHRGLAGIREEQIKK
                     LVIAYEPVWAIGTGLTASPLQAEEMCGIIRNTIDDLYSPEAGDSVIIQYGGSVKPANA
                     SEIMNMPQIDGCLVGGASLEPVDFMQIIDF"
     gene            complement(2295..3479)
                     /locus_tag="PLM92_04130"
     CDS             complement(2295..3479)
                     /locus_tag="PLM92_04130"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006000536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HPR24357.1"
                     /translation="MKKTVRDIEVKGKRVLVRCDFNVPINDLGITDDVRIKAALPTIE
                     YLIKNGAKVILMSHMGRPEGQAKMEYTLKPVAERLSTLLNKKVLFISCPEVISEKVRA
                     ASLELGEGDVMLLENTRFRKEETKNMDTFSKELSELGEVFVNDAFGTAHRAHCSTVGI
                     SAYVPSVSGFLMEKEIRFLGDALEEPERPFLAIMGGAKVKDKIPVIENLLKKVDSLII
                     GGGMAYTFFKAMGFEIGRSILDNENIELAGELIKKAEASGVKMLLPLDVLCSEEFSED
                     CRREICNNDSIPKDMMGMDIGPKTMELFTAEIASARTVVWNGPMGVFEMDSFAEGTKA
                     VAEAMAKSNALTIIGGGDSAAAVEKFGMAQKMNHISTGGGASLEFLEGRELPGIAVLE
                     DK"
     gene            complement(3502..4518)
                     /gene="gap"
                     /locus_tag="PLM92_04135"
     CDS             complement(3502..4518)
                     /gene="gap"
                     /locus_tag="PLM92_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005331598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HPR24358.1"
                     /translation="MAVKVGISGFGRISRVAIRAAMDDPEIDICGINVRNAEKDYMLY
                     MLKYDTTFGRFPKSLDMYEEGLVINGRKVPVYSESEAVNIPWKNCGAEYIVEGTGAYV
                     TTEKAMDHIKAGAKKVIISAPAKDTETPTFVMGVNHNEYKKEYQIVSNASCTTNCLAP
                     LAKVIHDNWTIEEALMSTIHAATAKQKVVDARSMKDWRTGRSAFGNIIPSTTGAAKAV
                     GLVIPDLKGRMTGISYRVPTADVSVIDLNVRLAKAATYEEMCSKMKEASEGPMKGILE
                     YVDDEVVSSDFIGDPCTSIFDSREGIMLNERFFKLIAFYDNEYGYSSKVLMLIKHMSS
                     VDNA"
     gene            complement(4595..>4710)
                     /locus_tag="PLM92_04140"
     CDS             complement(4595..>4710)
                     /locus_tag="PLM92_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014097469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HPR24359.1"
                     /translation="QTLVLLLKERGIDISRRTIAKYRDEMGISSSSKRKRY"
BASE COUNT         1189 a         1210 c          849 g         1462 t
ORIGIN      
        1 atgcccacac cgaatcctgc gattatggta gttgcaggac cggtctcgct ttgtcctgcg
       61 attttcttta ctgatttgta atctgaagaa gtgtaaactt ctgttatctt tcctattgct
      121 ataccgacga taagtcctcc tatgatagcc actgcatatt tgatgctgcc cagcatgaaa
      181 taactgagga cgaatgagaa tattattact attatgccac ttatatatgt gcccatatta
      241 agtgcagtgg caggattgcc cccttcttta cctcttacca tcagtattcc tattattgat
      301 gctattattc ctactgagga aagcaaaagc gggaacagag ccgctgccgt ttcatcgaaa
      361 aggcctcccg agtttgctac agccacagct gcgagagtta ttgcggaaat tgttgagccc
      421 acataagatt caaaaagatc tgaacccatt ccggcaacat cgcccacatt gtcacctaca
      481 ttatctgcta taacagcggg gtttctggga tcgtcttcgg gtataccggc ttccaccttg
      541 cctaccaggt ctgctcccac atctgcggcc tttgtatata taccgccacc tactcttccg
      601 aaaagagcca tggaagaagc cccgagtcca aatccggtaa tgaccgacac acagtcaaga
      661 cccattataa caaaaatcac gcccaggccc agaagaccaa gaccggttac gcaaagtccc
      721 attacagcac cgctcctgaa ggctatcttc agggctttgc tcatgccgga ttccattgct
      781 gcgtttgcag tcctgacatt gcctttggtg gcgactttca ttccgaagaa gccggccaga
      841 acggaaagac atgcacctac cacataaagt gccgcagtga tccagcttat tgcgaaccct
      901 attataagga aaagcaataa tatcacagcc accattgtct tgtactctct gcttagaaaa
      961 gccatagctc cttctcttat aaatccggat atctctttca ttctgtctgt gccttcatcg
     1021 gccctgccga tccaggctgt aaggccgtat gccactatca gccctatgat cgccgccaca
     1081 gggacgataa tcgctaatgc attcatgttt taaaacctcc ttaaactgct gataagaggc
     1141 atcttcccga cacctcttac ccacaataaa tattttacat attgatttga ttgaactgtt
     1201 ctcatgctgt atggctttcc agtgtaacta tgaccagtga cagcacaata aaaataacag
     1261 ctgcagttgt ggctatcctg ccaaggatag cttcatatcc tctgctcttt ttctttccga
     1321 aaagctgctc agcaccgcca cctatggctc ctgccaagcc ggcgctgttg ctggactgca
     1381 taagtatgct tatcatgagt attacactgg caactgccag tatcacttta aaaactatca
     1441 ttaaagtcgc cattgctgtt cctcctgtaa ctgtaacaaa atcacattcc gtcacagcca
     1501 tttgagttta acataagact atttaaaaat caaggatttt tctgcttcaa aaatctatta
     1561 tttgcataaa atcaacaggt tcaaggcttg ctccaccgac caggcatccg tctatttggg
     1621 gcatgttcat tatctctgat gcgtttgccg gtttcacact tcctccatac tgtataataa
     1681 cgctgtcccc tgcctccggg ctgtaaagat catctatagt atttcttatg atgccgcaca
     1741 tttcttccgc ctgcagcgga cttgcggtaa gtccggttcc tattgcccat acaggttcat
     1801 acgcaataac aagttttttg atctgttcct cccgaattcc tgcaagtccc cggtgaagct
     1861 gttttttgac tacttcagct tctgttcccg cttctctttg ttcaagcact tcaccaacac
     1921 acattatcgg aacaatggat gtttcaaaga gcttatgcag ttttttgttg acagtttcat
     1981 ctgtttcagc aaagtactgt cgtctttccg aatgaccgac aatacagtaa tccactccta
     2041 tctcttccag cataagagca gacacctctc ctgtaaaagc tccctgatct tcaaaatgaa
     2101 cattctgagc cccgaccttt atttgtgttc cctcaaatac tgcctttaga gtatcaaggt
     2161 gaacatatgg tgcgcatatg gctgtcctta catctgtatc cttataaagc tccttgaatt
     2221 ctcgtgcaaa aaccaccgtt tcgatgcgtg acttgaacat cttccagttg cctgcaataa
     2281 aaggtattct catatcattt gtcctccaaa actgctatgc ccggcaattc tctgccctca
     2341 agaaattcca gtgatgctcc acctcctgtt gatatatggt tcattttttg tgccattccg
     2401 aatttctcaa cagcagccgc agaatctcct ccgcctatta tggtcaaagc gttgcttttt
     2461 gccatggctt ctgcaacagc ttttgtgcct tctgcaaaac tgtccatttc aaacactccc
     2521 atggggccgt tccatactac cgtccgggct gaggctatct cggcagtaaa aagttccatt
     2581 gttttcggac ctatatccat acccatcata tcctttggta tactgtcatt gttacagatt
     2641 tcccttctgc agtcttcaga aaactcttcc gaacataaaa catccaaagg caacagcatt
     2701 tttactcctg aagcttcagc ttttttgatg agttctcctg ccagttcaat gttttcatta
     2761 tccagtattg acctgccgat ttcaaatccc atggctttga aaaaggtata tgccatgccg
     2821 ccaccgataa tgagagaatc tacttttttg agcaggtttt ctatcacggg tattttgtcc
     2881 ttgacttttg ctccgcccat tatggccaaa aaaggtcttt ccggttcttc aagggcgtct
     2941 ccaagaaatc tgatctcttt ttccataaga aaacccgaaa cagagggtac ataagctgaa
     3001 atgcccaccg ttgagcaatg tgccctgtga gccgttccga aggcatcgtt cacgaaaact
     3061 tctcccagct cagataactc ttttgagaaa gtgtccatat tttttgtttc ttcttttctg
     3121 aatctggtat tttcaagaag cataacatca ccttctccaa gttcaagtga tgcagccctt
     3181 accttttccg aaatgacctc cggacagctt atgaaaagga cttttttgtt cagcaaagtg
     3241 ctcagtcttt ctgcaacggg tttgagagta tattccatct tagcctgccc ctcgggcctt
     3301 cccatgtggg acatcaatat aacttttgca ccgtttttta tcagatattc aattgtaggc
     3361 aaagctgctt ttatacgcac atcatcggtt atgccaaggt cgttaatcgg cacattgaaa
     3421 tcacaacgca caagcactct tttgcctttg acttctatat ctcttactgt ctttttcatt
     3481 tgcagcctcc tttattgttt atcaggcatt gtccactgat gacatatgct ttatcagcat
     3541 aagcactttg gatgaatatc cgtactcatt gtcataaaaa gcaataagct tgaagaatct
     3601 ttcattcagc attattcctt cccttgaatc aaatattgat gtgcacggat cacctatgaa
     3661 atcactggag accacctcat catccacata ctccaatatg cctttcatgg gaccttccga
     3721 agcttctttc attttgctgc acatttcctc gtatgtcgct gccttggcca gtctcacatt
     3781 aaggtctatt actgaaacat ctgccgtggg cactctgtat gagatgcctg tcattctgcc
     3841 tttaagatca ggtataacaa gtcccactgc cttggctgct cccgttgtgg acgggattat
     3901 attgccgaat gctgatctcc cggttctcca gtctttcatt gatcttgcat caacgacttt
     3961 ctgctttgct gttgccgcat gtatggttga catcagggcc tcttctatag tccagttatc
     4021 atgtatgact tttgcaagag gtgcaagaca gtttgtcgtg cacgaagcat tggaaactat
     4081 ctgatattct tttttgtatt cattgtggtt gactcccatt acaaaagtag gagtctcggt
     4141 atccttggcg ggagcggaaa taataacttt ttttgctcct gcctttatgt gatccatcgc
     4201 cttctcagtg gttacataag ctcctgtgcc ctcgactata tactctgcgc cgcaattttt
     4261 ccagggtata tttactgctt cgctttcgct gtaaacagga acttttctgc cgtttatcac
     4321 aaggccctct tcatacatgt caaggctttt gggaaaacga ccgaaagtcg tatcgtactt
     4381 cagcatatac aacatataat ctttttctgc gttacgaaca tttatgccgc agatgtcgat
     4441 ttcagggtca tccatggctg cacggatcgc cactctgctt atacgaccaa aaccactaat
     4501 accaactttt actgccattt cgattcctcc tcatactctg ccccggggca gaacaaatga
     4561 aataaaacta tataattact atgatataac aaaatcaata acgctttctt tttgaagaag
     4621 aagatatccc catttcatca cgatactttg caatggtcct tctggaaata tctattcctc
     4681 tttccttcaa aagcagaaca agggtctggt
//
LOCUS       DAONHK010000087         4678 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_14274_length_4678_cov_5.028769, whole genome shotgun sequence.
ACCESSION   DAONHK010000087 DAONHK010000000
VERSION     DAONHK010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4678
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14274_length_4678_cov_5.028769"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(141..542)
                     /locus_tag="PLM92_04145"
     CDS             complement(141..542)
                     /locus_tag="PLM92_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HPR24360.1"
                     /translation="MIIQEAAEMYLETILILKKRNGSVRSIDIANEMNYSKPTVSEQM
                     KKFRQNGYIKMDPNGYITMTEKGLSIAENTYEKHLLLAGFLVEIGVDEKTAYEDACRI
                     EHYISHKTFECIKKHYRKLSPDKPASKLRSE"
     gene            693..899
                     /locus_tag="PLM92_04150"
                     /pseudo
     CDS             693..899
                     /locus_tag="PLM92_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
     gene            916..1137
                     /locus_tag="PLM92_04155"
     CDS             916..1137
                     /locus_tag="PLM92_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002593911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HPR24361.1"
                     /translation="MYTLKQAKTGTTVTAVKLHGGGAVKRRLMDMGITKGTEIHVRKV
                     APLGDPVEINVRGYELSLRKSDAGMIEIK"
     gene            1251..3410
                     /gene="feoB"
                     /locus_tag="PLM92_04160"
     CDS             1251..3410
                     /gene="feoB"
                     /locus_tag="PLM92_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HPR24362.1"
                     /translation="MNITIALAGNPNSGKTTLFNELTGANQFVGNWPGVTVEKKEGKL
                     KGDISSVNIIDLPGIYSLSPYSLEEIVARNYLLDQRPDAVINIVDGTNLERNLYLTTQ
                     LAEIGIPMVIAVNMIDIIRKNGDKIDIEKLSEELGFPVYEISALKGISTKEVADHAVR
                     MAKKRKKAMPKHSFKGSVEHAIAHIEEVALHSMPEEQQRWFAIKLFERDEKVYDQLSI
                     SPELRKHIETDIKNCEDEMDDDSESIITSSRYDYITSIINKCLFKKDKDRISLSDKID
                     RVVTHRVMALPIFAAVIFAVYYVSVSTLGAFLTDWTNETLFGERIMPAVSTLLEATES
                     AQWLNGLLVDGIIAGIGAVLGFVPQMLVLFIFLAFLEACGYMARIAFIMDRIFRKFGL
                     SGKSFIPMLIGTGCSVPGIMASRTIENDRDRKMTVITTSFIPCGAKLPIIALIAGALF
                     DGAWWVAPSAYFAGIAAIICSGIILKKTKMFAGDPAPFVMELPSYHWPATSNVLRSAW
                     ERGWSFIKKAGTIILLSTIVVWFTSYFGWINGKFVMLDDMQMENSILAYIGAAVTWLF
                     VPLGWGHWKAAVASVTGLVAKENIVGTFGILYGFSRVAEDGSEIWISLQGSFTPIAAY
                     SFLIFNLLCAPCFAAVGAIKREMNSGKWTWFAVGYQSLLAYSVSLCVYQFGRLINGEP
                     AGIWTFTAVLIVILLAYMMIRPDKRKRFFGKEIKNPV"
     gene            3487..3642
                     /locus_tag="PLM92_04165"
     CDS             3487..3642
                     /locus_tag="PLM92_04165"
                     /inference="COORDINATES: protein motif:HMM:NF024081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HPR24363.1"
                     /translation="MFATVIISILLAAAVAAAITVLYKGRKKGKPLCGCDCRNCARNS
                     ICKGEKS"
     gene            complement(3817..>4678)
                     /locus_tag="PLM92_04170"
     CDS             complement(3817..>4678)
                     /locus_tag="PLM92_04170"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPR24364.1"
                     /translation="RMLDEITGDHAAVILAENSAGIWINSSALDLVRNAAREDEVEVL
                     TIPYIITVLDPFDNDALKYAVNQQTFSWCQRGITTVFNSGSPEVFSEAYQSILISMLQ
                     EGFLRQRFFDSLSVFTNTNTRYLLSRLMHGKTLCTELDGFINFNTLKLNSENDNSKIN
                     DDYLEKVFAEISTRGFDMIVEDHNKKYRYLGPEEDPDIFCEENTSSDMDSYIRYRTVD
                     AAYQIGMSEILGSVEVGKKADFTVFEEDPLQKENLPEAFMTILNGDIVYDSEEDDINE
                     WYELMTSQQF"
BASE COUNT         1467 a          848 c         1033 g         1330 t
ORIGIN      
        1 tagccagggc ttatagccct agagcaaacc accctttgga agggtggtta tgattctttt
       61 atgtcaggta tgtccggtgt tcttttgtaa aaacattttt cggatattaa ctaagaattg
      121 atcatatacc gggttggagc ttattcagat ctcagcttgg atgcgggttt gtccggggat
      181 agcttccggt aatgcttttt tatacactcg aatgttttgt ggcttatgta atgttctatc
      241 ctgcaggcat cttcataggc ggttttttca tccacgccta tttcaacgag gaatcccgcc
      301 agaagcagat gtttttcata ggtgttttct gcaattgaaa ggcccttttc cgtcattgtg
      361 atatatccgt ttggatccat ttttatatat ccgttttgcc ggaatttttt catctgctcg
      421 ctgaccgtcg gttttgaata attcatttca ttggctatat ctatagagcg gacagaaccg
      481 tttctttttt ttaatatcaa aatagtttcc agatacatct cggcagcttc ctgaatgatc
      541 atagagcttt ctcctttcgt tttcctgatt tgattataac attagtattt tttgctatca
      601 acggaaaaat ccttgacaat ggagttagga gatgctaaca tttagttgag ttaggcgaga
      661 ctaaccaaaa aaatgcttac ggaggtgaaa atatgccgct gattatggca aacacaggag
      721 aaaaactttt tataaaaaaa gtaaatggaa aagaaaaaac aagaaaattt cttgaagata
      781 tcggatttgt tgaaggaact caaattacag tggtctcaag actgaaggaa gatctgataa
      841 tcaatgtcaa aggatctaaa atagcactaa gcagagaaat cgcttcaaaa attatagtat
      901 ggggaggaaa ttcgaatgta cacattgaaa caggcaaaga caggcaccac ggtcacggct
      961 gtaaagctac acggaggcgg tgccgtgaaa agacgcctta tggacatggg aataacaaaa
     1021 ggaacagaga tacatgttcg taaagttgcc ccgctgggag atccggtaga gatcaatgtc
     1081 agagggtatg agctttctct acgaaaatca gatgccggaa tgatagaaat caaataaaag
     1141 aaatcatatg ccggaaatca aaggataggt aaacagcatg cagaggcact gatgtctctg
     1201 caataaaaaa agcattgagt taggagacac taacacaaag gagggataaa atgaatatca
     1261 ctattgccct tgccggtaat ccgaattcgg gcaagacaac gctttttaat gaactaacgg
     1321 gtgctaatca gtttgtgggc aactggcctg gagtcactgt tgaaaaaaaa gaaggaaaac
     1381 taaaaggaga catatcttct gttaatataa tagatctgcc ggggatatat tctctttcgc
     1441 catacagtct cgaagaaata gttgcaagaa actatctgct ggaccagagg cctgatgccg
     1501 taataaatat agtggatggg actaaccttg aaagaaacct ttatcttaca actcaattgg
     1561 cggaaatagg aataccaatg gttatcgcag ttaacatgat cgatattatc cgtaaaaacg
     1621 gagataaaat agatatcgaa aaactttcag aagaactggg ttttcccgta tatgaaattt
     1681 ctgctctgaa aggaatatcc acaaaggaag ttgcagacca tgcggtaaga atggcaaaaa
     1741 aaagaaaaaa agccatgcct aaacacagct tcaaaggcag tgtagaacat gccatagctc
     1801 atattgaaga agttgcgttg cacagtatgc ctgaggaaca gcaaagatgg tttgcaataa
     1861 agcttttcga gagagatgag aaggtgtatg atcagctttc catatcaccg gagttgagaa
     1921 agcatattga aacagatata aaaaactgcg aagatgaaat ggatgatgat tcagaaagca
     1981 taataacctc atcaagatat gattatatta catcgataat aaacaagtgt ttattcaaaa
     2041 aagataaaga cagaatatct ttatcggaca agatagacag agtggtaact catagagtta
     2101 tggctcttcc catatttgca gctgtaattt ttgctgtata ttatgtttcg gtttccactt
     2161 tgggagcctt tcttacagac tggaccaatg aaactttgtt cggagaaagg ataatgccgg
     2221 ctgtatctac cctcctggaa gctacagaaa gcgcccagtg gcttaacgga cttttggtgg
     2281 atgggataat agcaggaatc ggagcggtac tgggattcgt ccctcaaatg ctggttctat
     2341 ttatttttct tgcattcctt gaagcatgcg ggtatatggc aaggatcgca tttataatgg
     2401 atcgcatatt caggaaattt ggtctttcgg gaaaaagctt cattcccatg cttataggaa
     2461 cgggatgcag cgtgcccggc ataatggcat caaggaccat tgaaaatgac agagacagaa
     2521 aaatgacggt tataaccact tcgtttatac cctgtggcgc taaacttccg ataatagccc
     2581 ttattgccgg agcattgttt gacggtgcat ggtgggtggc acccagtgca tattttgcag
     2641 ggatcgcagc cataatctgt tccggcataa tacttaaaaa aacgaaaatg tttgcaggag
     2701 atccggcccc ctttgtcatg gaacttcctt catatcattg gcctgcaact tcaaatgtgt
     2761 tgcgctccgc atgggagagg ggatggagtt ttattaaaaa agccgggacc attatcctct
     2821 tgtcaaccat tgtagtttgg ttcacatcat atttcggatg gataaacgga aagtttgtta
     2881 tgcttgatga tatgcagatg gaaaacagca tacttgctta tatcggagcg gctgtcacat
     2941 ggcttttcgt acctctggga tggggacact ggaaagctgc agtggcctct gtcacaggtc
     3001 ttgttgcaaa agaaaatata gttgggacat ttggtatatt atatggattc agccgggtag
     3061 cagaagacgg atctgaaata tggatcagcc tccagggttc attcacgcca atcgcggcat
     3121 attcttttct gatattcaat ctgctttgtg ctccctgctt tgctgcagtt ggtgccataa
     3181 aaagagagat gaacagcgga aaatggacct ggttcgcagt gggatatcag tcacttttag
     3241 cttattctgt aagtttatgt gtatatcagt tcggccgcct gatcaatggc gaaccggcag
     3301 gtatatggac atttacagca gttttgatcg tgatcttgct cgcatatatg atgatccgtc
     3361 cggacaaaag aaaaagattt ttcggaaagg agatcaagaa tccggtctga aaatttaaag
     3421 aagaaatatt aataaataat aagcagggta aaatgtttat atacagagaa aaaggaggaa
     3481 aataaaatgt ttgctactgt aataataagc atactgctgg cagcagctgt ggcggccgcc
     3541 ataacggtgc tttataaagg cagaaaaaaa ggaaaacctt tatgcgggtg tgactgcagg
     3601 aattgcgcaa gaaacagcat atgcaaagga gaaaagtctt aggcttaaat acaaatatcc
     3661 cgagggggat cccgggatat ttgcagtgtg tgtattcaat aaaagaagga aagtttgtat
     3721 gtgagaaatc catacttctg atccttcttg agtatagaac gaaaaagtga caaatatgtg
     3781 aaaaaaagag ttgataagat ttctttcaca aaatcttcaa aattgctgac ttgtcatcag
     3841 ctcataccat tcatttatat cgtcctcttc agaatcgtaa actatatctc cgttaagtat
     3901 tgtcataaac gcttccggaa gattttcttt ttgaagagga tcctcttcaa aaacagtaaa
     3961 atcagccttc tttccgactt ctacagaacc gagaatttca ctcatgccta tttgataagc
     4021 tgcgtcgaca gtacgatacc tgatatatga atccatatcg gaagaagtgt tttcttcgca
     4081 gaatatatcc ggatcttctt ccggaccaag atacctgtat tttttattat gatcttctac
     4141 gatcatatca aaacctcttg tggaaatttc agcaaaaacc ttctcaagat aatcatcatt
     4201 gattttgctg ttatcatttt cactgtttag tttaagagta ttgaaattga taaaaccgtc
     4261 aagctcggtg cataatgttt ttccgtgcat aagtctggaa agcagatacc ttgtatttgt
     4321 atttgtgaat acagataatg aatcaaaaaa tctttgtctg aggaatccct cctgaagcat
     4381 ggagatcaga atgctttgat aggcttcgct aaaaacctcc ggggaaccgc tgttgaaaac
     4441 ggtggtgatc ccccgctgac accatgaaaa agtctgttgg ttgacggcgt atttaagggc
     4501 atcgttatca aagggatcaa gcactgtaat gatgtaaggt atagtcagca cttcgacctc
     4561 gtcttccctg gcagcattcc tgaccaaatc aagggcggaa gagttgatcc atataccggc
     4621 actgttttct gcaagtataa ccgcagcatg atctccggtt atttcatcca gcatacgg
//
LOCUS       DAONHK010000088         4663 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_14342_length_4663_cov_4.074436, whole genome shotgun sequence.
ACCESSION   DAONHK010000088 DAONHK010000000
VERSION     DAONHK010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4663
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14342_length_4663_cov_4.074436"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(52..882)
                     /locus_tag="PLM92_04175"
     CDS             complement(52..882)
                     /locus_tag="PLM92_04175"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HPR24365.1"
                     /translation="MMTKVTVLGGIEVYIKNDPSGQIIRGMDNPGRISMRFAGPGFET
                     VEGLCREEADVYFCAAVGNDLAGRAVKAEVAALGANGDGITLIEGYDTAASHGVFNIV
                     NDLEFAFSNHDIYEKMEKSMIERVLPSIKKSDIVSADTTLPLDIIEYIRDECRDTLIM
                     LDVISEKTAAKTKEICGSFDIVKADRQSAEVLSGMEILSQEQLEKAAAEIMKKGMKKL
                     FITLGPGGIYYRQGDERGTLHSDRDTAVSDRTKTSARLIMGCAQGLSSSETAEKAIKE
                     "
     gene            complement(920..2521)
                     /locus_tag="PLM92_04180"
     CDS             complement(920..2521)
                     /locus_tag="PLM92_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005998668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HPR24366.1"
                     /translation="MKIKLKKDIKSEFETIEVKKDITLEKLAGSIKRDVPYMILAAKV
                     DNKVKRLTGIISDPCEIEFLDMRTRQAHLIYQYSVTLLYLAAVKEVLETTDTEIRNSL
                     NQGLFTLIRTKRPLTASDVKAVEKRMRQMVREDIPIESEGACKKEELKQMQLYRIKDY
                     EQFFYGLTVPSSGYLKYFLLKKYKRGVILRFPQPSMPDKMPPYVDNKKMYHAFAEAEK
                     WQRLLDISHVWDLNKKIIGGETREIIQMSEALHDKRIVEIAGEIAKLKKRVILIAGPS
                     SSGKTTFARRLCIQLKVNGLKPLYLGTDDYFLDRKDTPKDENGEPNFEDLEALDIDLF
                     NRDMNMLLEGKEADIPEFDFLQGKKVFGNRRTSIDKTNPIVIEGIHALNDELTPYIDD
                     ERKFKIYISPLTQLNIDDHNVISTTDSRMIRRMVRDHQYRGHSAEATIKSWPKVRAGE
                     DKNIFPYNSSADAFFNSVHIYELGVLKKYAEPMLKEVGEDRPEYSEAVRMLKFLSFFK
                     TIEDDSLIVNNSILREFIGGSVFHQ"
     gene            complement(2649..2885)
                     /locus_tag="PLM92_04185"
     CDS             complement(2649..2885)
                     /locus_tag="PLM92_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011170760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PC4/YdbC family ssDNA-binding protein"
                     /protein_id="HPR24367.1"
                     /translation="MSEKEKTEIVFEIREHLGVIGAYPTGWQKELNLVAWNGTPPKYD
                     IRDWDHDHEHMSRGITMHRDEMKKLVQIVSDKEF"
     gene            complement(3035..4444)
                     /locus_tag="PLM92_04190"
     CDS             complement(3035..4444)
                     /locus_tag="PLM92_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013378384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPR24368.1"
                     /translation="MMTMDKVKAFGAKRAIEYVEKDYKKNAPKLLDWLIEHDIGGDSL
                     APQAKIAKKHMQDPESNWNKLAEKVWTEVDAGVRKKIVENFIIHASLSGSKKQFETME
                     KEKCNVPWAILLDPTSACNLHCKGCWAAEYGDKLNLTFDELSSIVEQGKAMGTYFYIF
                     TGGEPLVRKDDVIKICEKHNDCAFLSFTNATLIDEAFAEEMLRVKNFIPAISIEGFKK
                     ETDARRGEGTFDSIIEAMDILKKKKLPFGVSCTYTSENTETIGSSEFIDFLIEKGALF
                     AWLFTYMPVGKGAAEELMVNSEQRKYMYEQIREFRNTKAIWTMDFWNDGEFVGGCIAG
                     GRSYLHINANGDIEPCAFIHYSDSNIRKHTLLEAYKRPLFMEYYNNQPFSDNMLRPCP
                     LLDNEGALAQMVKRSKAHSTDVNDPEDVDELTAKCKNAATAWKPVADEIWASKPHHEY
                     SGPRHNTRFHDDKYFETEK"
BASE COUNT         1073 a         1073 c          865 g         1652 t
ORIGIN      
        1 cagcctgttt gctgctttta ctgcatctga catttttctc ctcctgtctt tttactcttt
       61 tatagccttt tctgctgttt ctgaggacga gagcccctgt gcacatccca ttataagccg
      121 tgcggaagtt ttcgttctgt cggatacggc tgtgtcccgg tcactgtgca atgtgcccct
      181 ttcgtccccc tgccgataat atatgccacc gggtcccagc gttataaaca gctttttcat
      241 acctttcttc attatttccg ccgctgcctt ttccagctgt tcctgactta atatttccat
      301 tccgctaaga acctcggctg actggcggtc agcctttact atatcaaaag atccgcatat
      361 ttcttttgtt tttgctgcgg ttttttctga aataacatcg agcattataa gtgtatctct
      421 gcattcatct ctgatatatt caattatatc aaggggcagc gtggtgtctg ccgaaacgat
      481 atctgatttc tttattgaag gaaggactct ctctatcatg ctcttttcca tcttctcata
      541 tatgtcatgg tttgaaaatg caaattccag gtcattcact atattgaaaa cgccatggct
      601 tgctgcagta tcatatcctt caataagtgt gataccatct ccgtttgccc ccaaagccgc
      661 aacttctgct ttgactgctc ttcccgccag atcattgcca actgccgcac agaaataaac
      721 atcggcttct tccctgcaca gtccttcaac tgtctcgaag cccggtccgg caaacctcat
      781 ggatattctc cccgggttat ccatacctct tatgatctgt cccgacgggt catttttgat
      841 ataaacttct attcctccta atactgttac cttcgtcatc atttctcctt gctatgtatc
      901 aatgcattat cttatatttt tactgatgga agacgctccc tccgatgaat tcccttaaga
      961 tagagttgtt tacgatcagt gaatcgtctt cgatggtttt gaaaaagctt agaaacttaa
     1021 gcattctgac tgcttcgctg tattcgggac ggtcttctcc cacttccttt agcattggtt
     1081 ccgcatattt tttcagtacg cccagctcat atatatgaac cgagttgaaa aaagcatctg
     1141 cggaactgtt gtaaggaaaa atattcttgt cttcgccggc tcttactttt ggccagcttt
     1201 ttatggtggc ttcggcagaa tgacctctgt actgatgatc tctgaccatt cttcttatca
     1261 tccttgaatc agttgtggat atgacattat gatcgtctat gtttagctga gtcagagggc
     1321 ttatatatat cttgaattta cgttcatcat ctatataggg tgtaagttca tcattcaaag
     1381 catgtatacc ctcaatgact attggattgg ttttgtctat ggatgtcctc ctgtttccga
     1441 atactttttt gccctgaaga aaatcaaatt caggtatgtc tgcctctttc ccctccagca
     1501 gcatattcat atctctgttg aaaagatcga tatccagcgc ttcgagatct tcaaagttcg
     1561 gctcgccatt ctcatctttg ggtgtatctt ttctgtcaag gaaatagtcg tctgtcccca
     1621 gataaagcgg tttcagtccg ttgactttca gttgtatgca aagtctcctg gcaaaggtgg
     1681 tcttgccgga agaagaaggt cctgcgataa gtataactct cttttttagt tttgctattt
     1741 ctccggcgat ctcaactatt cttttgtcgt gaagtgcttc tgacatttgt attatttctc
     1801 tggtttcacc tccgattatt tttttgttaa gatcccaaac atgggatatg tccagcagtc
     1861 tctgccattt ttccgcttcc gcaaaagcat gatacatttt tttgttatcc acatagggcg
     1921 gcattttgtc aggcatggag ggttgaggga atctcaatat cactcctctt ttatattttt
     1981 tgagcaggaa atatttcagg tatcctgaag aggggactgt gagtccatag aagaactgtt
     2041 catagtcctt tattctatat agctgcatct gtttcagttc ttcttttttg catgcgcctt
     2101 cagattctat gggaatgtcc tctcttacca tctgcctcat tcttttttct acagctttaa
     2161 catcggatgc agtcagaggt ctctttgttc ttatcaatgt aaaaaggccc tgattaagag
     2221 agttcctgat ctctgtgtca gtcgtttcaa ggacttcctt aacggcagca aggtaaagaa
     2281 gggtaacact gtactggtag atcagatggg cctgtctggt gcgcatatca agaaactcta
     2341 tctcacatgg atccgatatt atcccggtaa gtcttttcac cttgttgtcc accttcgctg
     2401 ccagtatcat ataagggaca tctcttttta tgctcccggc cagcttctca agggtaatat
     2461 ccttctttac ttctatagtt tcaaattctg attttatgtc tttttttaat tttattttca
     2521 tttgtccctt ccccctacag cttagcatat atttttatat ataaaggaag tataacacag
     2581 ggaaaaaggc ggtacaaccg aaagccgcag cgccttttca tgcaaaataa cgtttccaag
     2641 aaactctctt aaaattcctt gtcacttacg atttgaacga gcttcttcat ttcgtctctg
     2701 tgcatggtta ttcctcttga catgtgctca tgatcatggt cccagtctct gatatcatac
     2761 tttggagggg tgccgttcca ggcgacaaga ttaagttctt tctgccagcc tgtaggataa
     2821 gcccctatta cacccagatg ttccctgatc tcaaatacta tttctgtttt ttctttctca
     2881 ctcacagtgt ttgttctcct ttcttgatat tttatatttg acaatattat acatttaatg
     2941 gaaatatttg tcaacataat ttttctgtat aaaaacacgc ccttaaaaag ggcgtgcact
     3001 tcttataaaa agtgttttat tttacagcgg atatctattt ttctgtctca aagtatttat
     3061 catcgtgaaa tctggtgttg tgccttggac cgctgtattc gtggtgaggt ttcgatgccc
     3121 atatctcatc tgccacaggt ttccatgccg tagcggcatt tttgcatttt gcagtaagtt
     3181 catccacatc ttccggatca ttcacatctg ttgaatgggc tttggatctt ttcaccatct
     3241 gtgccagggc accttcgttg tccagaagag gacacggtct tagcatattg tcgctgaagg
     3301 gctggttgtt gtagtattcc atgaacagcg gccgtttata tgcctcaaga agcgtatgct
     3361 ttcttatgtt ggaatcggaa taatgtatga aggcacaggg ttcgatatct ccgtttgcat
     3421 ttatatgaag ataggatctt cctcctgcta tgcatcctcc cacaaattct ccgtcgttcc
     3481 agaaatccat agtccatatg gctttagtat ttctgaactc tctgatctgc tcatacatgt
     3541 acttcctctg ttcagagttg accatcagtt cttctgcagc gcctttgccc acgggcatat
     3601 aggtgaacag ccatgcaaac agtgctcctt tttcaatcaa aaaatcaata aattcgctgc
     3661 ttcctatggt ttctgtgttt tcagatgtat atgtgcatga tactccaaag gggagtttct
     3721 tttttttcag tatgtccata gcttctatta tggaatcaaa agtgccttct ccccttctgg
     3781 catcagtttc ttttttgaat ccctctatgc ttatggcagg aataaaattc ttgactctga
     3841 gcatctcttc tgcgaaggcc tcgtcaatca atgttgcatt tgtaaacgaa agaaatgcac
     3901 agtcattatg tttttcgcag atctttatta cgtcatcctt tcttacaagg ggctctcctc
     3961 cggtaaagat ataaaaataa gttcccatag ccttgccctg ttcaactatt gaacttaatt
     4021 cgtcaaatgt caggtttagt ttgtctccgt actctgctgc ccagcagcct ttgcagtgga
     4081 gattacatgc ggatgtggga tcaagcagta tggcccacgg tacattgcat ttttcctttt
     4141 ccattgtctc aaactgcttc ttggaaccgg agaggctggc atgtattatg aaattctcga
     4201 ctattttctt tcttactccg gcatccactt cagtccagac tttttctgcc agcttgttcc
     4261 agttgctttc cggatcctgc atgtgtttct ttgctatttt tgcctgggga gcaagggaat
     4321 ctcctccgat atcatgttca ataagccagt caagaagttt aggcgcattt ttcttgtagt
     4381 ctttttccac atactctatg gcccgttttg ctccaaaggc ttttacctta tccattgtca
     4441 tcatttttcc catttttact ctctccttct gcgagtttca ctgttcaaaa atgtatgttt
     4501 atctatacaa tcttaccgtt tttgcaggct tgcgtcaaat cagcttcgga aaatcaagat
     4561 tttagcaata atagacaaca aaaaaacgcc ttaacggcgt tttttttatg gagcggatga
     4621 ggaggatcga actcccgtat ccagcttggg aagctgatat tct
//
LOCUS       DAONHK010000089         4625 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_14513_length_4625_cov_5.866521, whole genome shotgun sequence.
ACCESSION   DAONHK010000089 DAONHK010000000
VERSION     DAONHK010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4625
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14513_length_4625_cov_5.866521"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="PLM92_04195"
     CDS             <1..598
                     /locus_tag="PLM92_04195"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HPR24369.1"
                     /translation="ARTGIGTITLVDGDVFDESNLNRQTVCNEKNIGKKKASETKKRI
                     EQINSEVEVTAIDEAADEKNSKEILEDCDIVFDAVDSIPARMLIEKKAEEAGVPLVHG
                     AVNGWYGQVCVIYPGDRIIEKIFSDGAAAAVNTKEGDRHGNEGAEAGAPAFTPAAVAS
                     VQTAEGIKVLLKKGSILRKKLLTMDLMEQEYELIKLDG"
     gene            complement(622..870)
                     /locus_tag="PLM92_04200"
     CDS             complement(622..870)
                     /locus_tag="PLM92_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006520830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPR24370.1"
                     /translation="MAKKLNTKNNKEDFAKIDENDAGTIVCTKCNVSMEPIDVTFKYL
                     KRSFRHKVLRCSKCGQVYVPEDLAKGRMAEVEAALEEK"
     gene            complement(842..3130)
                     /locus_tag="PLM92_04205"
     CDS             complement(842..3130)
                     /locus_tag="PLM92_04205"
                     /inference="COORDINATES: protein motif:HMM:NF026042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HPR24371.1"
                     /translation="MEKYLPNFKKCIQKEPPFCTAECPFHLDIIDFIEKIKTARFNAA
                     YKLYKNAVGFPEIAASLCHAPCKNVCPITNNPIELNLLEHAVIENAGIKEPTDYNLPK
                     RKQKIAIIGAGISGMSCALRLSTKRYDVSVFEKIDRIGGELWDIVDPEIFRKEFDLQF
                     RHLSCEINLQTEIRSLDELKDFDAVYVATGKGGSDFGLLQKHLDKPCFMQEDTGIFAG
                     GSILGKEKIYAQADGLKMATTIDNFLRTGNLIYTPDPKGTNTILDKERLIITTRVTPA
                     NKSSYTEEEAISEADRCLKCQCDACRLYCDLTEYERKWPLRIRDEIQATTLPGFSEVK
                     ATPAKRLISACTQCGLCVETCPKDIDIGGLILEARKSMHRQDKLAWVFNEFWLRDMDF
                     TNGPMAGLVKGSPGSNELCKFAFFPGCQLGAGEPDLVIKSYQYLLDREKSTGLILGCC
                     GIPAEWAGNDDKLNEEISRINTAWQTLGKPTFVLACPTCANHLKKYLPDIPCVFLYEI
                     MDKEGISFSGNSLPVKEYPGTYSVFDPCSTRGNRALRESVRAIAQKAGYKLKPLVHQE
                     KYSSCCSYGGQGSIADPGFAKFVREKRIKESDYPYICYCINCRDSFVEEGKSAFHILD
                     LLFGNKPSNPTVTKRRENRIFLKETILKDFWGEEMEEKKKSYSFRLFVNEDLKKKISN
                     DRILEEDVMEVLEFCERTGRTVYNKNTGVFSGYNMVGHMTLWIEYLKKNDESFELVNA
                     YTHRMEIELEDVWNGEKVKHEK"
     gene            complement(3256..>4625)
                     /locus_tag="PLM92_04210"
     CDS             complement(3256..>4625)
                     /locus_tag="PLM92_04210"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HPR24372.1"
                     /translation="AYDELGDDLTWRTARFTYFPYNVPNAMGLSRVATTNHAYGTAYR
                     GYGSPQAYTCSEAMMDMMAEKIGMDPFEIRWRNIAREGDLNVNQYPFVDYPMEQIMET
                     MKPIYDKAVERAKKEDTPEKRRGVGIAWGGYNVTEGNEDQCTLAIEIAPDGKFIKYDT
                     WQDQGQGGDSGSLQCTLEALKPLGVKKEDILLRQNDSKNGVDSGASASSRQHYMNSKT
                     SQLVADKMINAMKKTDGTYRTYDEMVAEGLPLRYEAQYENVTDPTLCALDPNTGVGNP
                     TPAYTYGLFLAEVEVDVATGKTKVIGYTSVDDVGVIGNPTSVDGQAYGGLSHCMGFAL
                     TEDYDDVKKHSNMAGAGLLPIGEIPDDFNLIHLENPRSTNPYGSSGASEAFQSAGHMA
                     VINAINNACGVRIYELPARPEKVKAGLEKLAKGEKILPPKKFFLGSDFYEEMENIKNN
                     PVDVD"
BASE COUNT         1165 a         1039 c          998 g         1423 t
ORIGIN      
        1 ggccagaaca gggatcggaa cgataacact tgttgacggg gatgtctttg atgaaagcaa
       61 ccttaaccgt cagacagtgt gtaatgaaaa aaacataggg aaaaagaagg cctcagaaac
      121 aaaaaaaaga atagaacaga ttaattcaga ggttgaggta acggccattg atgaagcggc
      181 ggatgaaaaa aactccaaag aaattctcga ggattgtgat attgtttttg atgccgtcga
      241 cagcatcccg gcaagaatgc ttatcgagaa aaaagcagaa gaagccggag ttcctctggt
      301 acatggtgca gtaaacggct ggtatggcca ggtatgtgtc atatatccgg gggacagaat
      361 aatcgaaaag attttcagcg acggggccgc agcagcggta aataccaaag agggggacag
      421 acacggaaat gaaggagccg aggcaggcgc tccggctttt acccctgcag cggttgcttc
      481 ggttcagacg gccgaaggaa taaaagtgct gctaaaaaag ggaagcatac tcagaaaaaa
      541 actcctgacc atggatctca tggaacagga gtacgaactc ataaaactgg atggatgaaa
      601 acgcaaagaa aagcctccgc gtcacttctc ctccagggca gcttccacct ccgccatgcg
      661 gcccttcgct aaatcttcag gtacatagac ctgcccgcat ttagaacacc gcaaaacttt
      721 atgccggaat gaacgtttta aatatttgaa agtcacgtct attggttcca ttgagacatt
      781 acatttggtg caaacaattg tgcctgcatc attttcatca atttttgcga aatcttcttt
      841 gttatttttc gtgtttaact ttttcgccat tccatacatc ctccagttct atttccatcc
      901 tgtgagtata agcgtttaca agctcaaagc tctcatcatt ttttttcaga tattcgatcc
      961 atagagtcat atgacccacc atattgtagc cgctgaaaac acctgtattt ttgttataga
     1021 ctgtgcggcc ggtcctctcg cagaattcaa ggacttccat cacatcttct tccagtatgc
     1081 ggtcattact gatttttttc ttcaaatctt catttacaaa cagccggaag ctgtatgatt
     1141 ttttcttttc ttccatttct tcaccccaga aatcttttag tattgtttcc ttcaaaaata
     1201 tgcggttctc cctgcgcttt gttaccgtgg gatttgacgg tttgttgccg aaaagaaggt
     1261 caagtatatg aaaagcgctc tttccttctt caacaaaaga atcacggcag tttatacagt
     1321 aacatatgta tggatagtcg ctttctttta ttcttttttc acgtacgaac ttggcaaagc
     1381 cggggtctgc tatagaaccc tggcctccgt agctgcagca tgaagagtat ttttcctggt
     1441 ggaccagagg tttcagttta tatcctgctt tttgtgcaat ggcacgtacc gattcccgca
     1501 gtgcccggtt gccccttgtt gaacatgggt caaagactga atatgtaccc ggatattctt
     1561 tgaccggcag agaattcccg gaaaatgaga taccttcctt atccatgatc tcataaagaa
     1621 aaacacaagg tatatcggga agatatttct tcaggtgatt tgcacaggta ggacaggcca
     1681 ggacaaaggt cggttttccc agagtctgcc atgcagtgtt tatccttgag atctcttcat
     1741 taagtttatc atcatttccc gcccattccg cagggatgcc gcagcatccc aggatcagac
     1801 ctgtggattt ttcccgatca agcagatatt ggtacgactt tattacaaga tcaggctcac
     1861 ccgcccccag ttgacatcct gggaaaaaag caaatttgca tagctcatta cttccggggg
     1921 accctttcac aagaccggcc ataggcccgt ttgtaaagtc catgtctctc aaccagaatt
     1981 cgttgaagac ccaggcaagt ttatcctgac gatgcatgct tttacgtgct tccagtatca
     2041 ggccgcctat gtcaatatcc ttcgggcatg tttctacaca cagcccgcac tgggtgcatg
     2101 cactgataag ccgtttcgca ggggtagcct tgacttccga aaagccgggc agagtagttg
     2161 cctgtatctc atctcttata cgcagaggcc attttctttc gtactccgtc agatcacagt
     2221 agaggcggca ggcatcacac tgacatttaa gacagcgatc tgcttcactg atggcttctt
     2281 cttcagtata ggagcttttg tttgccggcg tcaccctggt ggtgattata aggcgctctt
     2341 tgtccagtat ggtatttgta ccttttggat cgggagtata tataagattg cctgtcctga
     2401 ggaaattatc tatcgtggta gccattttga gcccgtcggc ctgggcatat atcttttctt
     2461 ttccaaggat ggagccgccg gcgaatatgc cggtatcttc ctgcataaaa cagggcttat
     2521 caagatgttt ctgaagaagt ccgaaatcag atccgccttt tcccgtggcc acatatactg
     2581 catcaaaatc ttttagttcg tcaagggatc ttatttcggt ttgaaggttt atttcacagg
     2641 aaagatgtct gaactgaaga tcaaattctt tcctgaaaat ttcaggatct actatgtccc
     2701 acaattctcc tccgatccgg tcgatttttt cgaagaccga aacatcgtat ctttttgtgg
     2761 aaagcctcaa tgcacaactc atcccactta tacctgcacc tattattgct attttttgtt
     2821 ttcgtttggg gaggttataa tcagtgggtt cttttatacc ggcattttcg ataacagcat
     2881 gttccagaag attgagctca attggattat ttgttatcgg acaaacattc ttgcatggtg
     2941 catggcataa tgaagctgct atttcgggaa atcccacagc attcttgtat agtttataag
     3001 cagcgttaaa acgtgctgtt ttgatttttt ctatgaaatc gataatgtcc agatgaaagg
     3061 gacattccgc agtacaaaaa ggcggttctt tttgaatgca ttttttgaaa ttgggcaggt
     3121 atttttccat gttttccttt ttataaaatt tatttacgat atatatttat tccggataga
     3181 atttgtgcgg cccgaaatgc tcaggccgca ctgttagtct aaactatttt gcttgttttg
     3241 taaaacaact gtattttagt caacatctac aggattgttt ttgatattct ccatttcttc
     3301 atagaaatcg gatcccagga agaatttctt tggaggcaag atcttttcgc cttttgccag
     3361 tttctcaagt ccggctttca ccttttcggg tcttgcggga agctcatata tccttacacc
     3421 gcaggcattg ttgatggcat ttattactgc catatgtcct gctgactgga aggcttcgga
     3481 agctccggat gaaccgtagg ggttggtgct tctgggattt tcaaggtgta taaggttgaa
     3541 atcatcaggt atttctccta ttggaagcag tcctgcacca gccatatttg agtgtttttt
     3601 cacatcgtca taatcttctg tgagagcaaa tcccatgcag tgtgagagac cgccgtaggc
     3661 ctgtccgtca acggaggtgg gatttcctat aacgcccaca tcatcaacgg atgtgtaacc
     3721 gattactttt gtttttcctg tggcaacatc tacttctact tcagccagga ataaaccgta
     3781 agtatatgca ggagttggat ttcctactcc tgtatttgga tccaaagcac aaagtgtggg
     3841 atcggtaaca ttttcgtatt gagcctcata acgaaggggt aagccctctg ccaccatttc
     3901 atcgtatgtc ctgtatgtgc cgtcggtttt cttcatggca tttatcattt tgtcagctac
     3961 cagctgggat gtcttgctgt tcatgtaatg ttgtcttgaa gatgcggatg cacctgaatc
     4021 gactccgttc ttgctgtcat tctgccttaa aagaatatct tcttttttaa cgcctagagg
     4081 tttcaaagcc tcaagagtac actgcagtga gccggaatct cctccctgac cctgatcctg
     4141 ccatgtgtca tattttatga acttgccgtc aggagcgatc tcgatggcca gtgtgcattg
     4201 gtcttcatta ccttcggtaa cattgtatcc gccccatgcg atacctacac ctcttctctt
     4261 ttcgggggta tcttctttct tggctctttc tacagccttg tcatatatgg gcttcattgt
     4321 ttccatgatc tgttccatgg ggtaatccac aaaaggatac tgattgacat taaggtctcc
     4381 ttctctggct atgtttctcc atctgatctc gaaaggatcc attccgatct tttcggccat
     4441 catatccatc attgcctctg aacaagtata agcctgaggt gaaccgtatc ctctgtaggc
     4501 agtcccgtag gcatggtttg ttgttgctac tctggaaagt cccattgcgt ttggaacgtt
     4561 gtatgggaaa tatgtgaatc tggctgttct ccaggtaagg tcatctccaa gctcatcata
     4621 agcac
//
LOCUS       DAONHK010000090         4538 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_14884_length_4538_cov_3.710908, whole genome shotgun sequence.
ACCESSION   DAONHK010000090 DAONHK010000000
VERSION     DAONHK010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4538
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14884_length_4538_cov_3.710908"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..714)
                     /locus_tag="PLM92_04215"
     CDS             complement(<1..714)
                     /locus_tag="PLM92_04215"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HPR24373.1"
                     /translation="MLFESIKFIDNLPFTVDFTNVLEENYHCHKEMEMLFVLKGRTKY
                     KIHNVHYDLSAGDIIIADVEDLHQIYGSSKDICMLQMHIDMKYFEKYHPDISYMFFVC
                     EECTKDSSSDPNDLQNKLSFLRKHLARIMISYCRDNDDSSLLMQEIKELIDILVSNFR
                     GYNMEDFQYKPDSSEINPIDLERLCRINKYLLNNYDKKITLGDVAEMEHLNPYYVSHL
                     IKNISGTGFQSFLNSLRVEF"
     gene            916..1455
                     /locus_tag="PLM92_04220"
     CDS             916..1455
                     /locus_tag="PLM92_04220"
                     /inference="COORDINATES: protein motif:HMM:NF015674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HPR24374.1"
                     /translation="MQLFKQMKVIGIIIGALIFIIGVVFVARPVKVEILLDWVLGISL
                     MIGGISMIGKVIAERNEKGNIFIRMIPGIALLVIAAFALINEGLTVFMVGIIIGIAAF
                     FMAFERLTVANERRKAAQSWGTVAIFGVIQIFFGAFMLYATFSMIAALIMIIGIYLMI
                     TGISLVISSAIFPLPKGEK"
     gene            1452..2729
                     /locus_tag="PLM92_04225"
     CDS             1452..2729
                     /locus_tag="PLM92_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006000432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HPR24375.1"
                     /translation="MIPLSTRMRPLGLDEFVGQEHFMYHGSLLYNAIKNKTFDSAIFF
                     GPSGTGKTTLARIVAREMDSDFVEINAGVSGTKELKEIIGAAKTKFFGLQKEKTYLYV
                     DEFHRWNKLQQDSLLKALEEGVLRFIGSTTQNPYFAINNAVLSRVRSIYEFRPLSVDN
                     ILVLLKRTLDDKERGFGNLRISYEEKALKNLADMSEGDARIALDTLGFIIDNLKQEGK
                     IDNGMIREAMQKRTVFYDKSEDKYNLLSALQKSIRGSDPDASIHYLARLIEGGADEMM
                     IGRRLMVIASEDIGMAYPNAIAVVTACVQAAHMVGFPEARINLAEAVILLASCPKSNR
                     AYMALESAQKDLENRKTGDVPPVLKDSHYSGAKKRGIGEGYKYPHDYGGYVKQQYLPD
                     ELHKDGVRYYEPTENGSEASFKKYLESLREKKK"
     gene            2726..>4538
                     /locus_tag="PLM92_04230"
     CDS             2726..>4538
                     /locus_tag="PLM92_04230"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF000907.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase/30S ribosomal protein S1"
                     /protein_id="HPR24376.1"
                     /translation="MTEIILSPNSGFCFGVKLALEKAEEAIRTRKDKKIYTCGPLIHN
                     RNVTDGLKEKGIEIIDSPAEAGNGSVVIVRSHGEPESFYKMASERDLEIVDATCPFVF
                     RIHMLVKEAWSSGMQVVIVGDKDHPEVIGINGWCGNSACVFSSGEEAAEMESSKKLFV
                     VAQTTITKNLFEEVVGELSSKNFEMEVKNTICSATSERQDGCLVTAKSSDAMVIIGGR
                     SSSNTTKLYEIAKKHCKNVYFVENIDDLPLKEIQKYNRIGVAAGASTPENAIKEVISN
                     MSDNITENKELSMDDLMEDIEKSLRLPRGGEMVTGKVHQVTDKEIIVNLGCKKDGIIP
                     LEEIKLEGDQKLTDLYSEGDEIQAKVLKTDDGDGSILLSKKKLEAGEHWEQINDALEN
                     KTFIDVEVLRQVNGGVIAAYKEVQGFIPLSQLSDRFIEDSSEFVGQTLTVRVSRVDQR
                     RGKAVFSRKAFLNEERQKLVEEIWESVNVDDIVEGTVMRFTDYGAFVDIGGIDGLLHI
                     SEISWGKLKHPKEVLEIGQKIHVKVLSMNNEKGKISLGLKQTQPEPWSIINDKYNEGD
                     IVKGKVVQIKEYGCFVELEPGLDGLVHISEVSHRRVGN"
BASE COUNT         1484 a          822 c         1083 g         1149 t
ORIGIN      
        1 aaactcgacc cgcagggaat tcaggaaact ctgaaagccg gtaccggaaa tatttttgat
       61 cagatgagaa acatagtacg gattcaggtg ctccatttcg gccacatccc cgagagtgat
      121 ttttttgtcg taattgttca gcaggtattt gtttattctg caaagcctct ccagatcgat
      181 gggattgatt tccgaagagt cgggtttgta ttgaaaatcc tccatattgt atccccggaa
      241 atttgatacc aggatgtcta taagttcttt tatttcctgc atcagcagag aagagtcatc
      301 attgtccctg cagtatgaga tcattattct tgccaggtgc tttctgagga aggaaagttt
      361 gttctgaaga tcattgggat ccgaggaaga gtctttggtg cattcttcac acacaaaaaa
      421 catatagctg atatcgggat gatatttttc gaaatatttc atatctatat gcatctgcag
      481 catacagata tcctttgagg aaccgtagat ctgatgaaga tcctctacat ctgcgattat
      541 tatatctcct gcggaaagat cataatgaac attgtgtatt ttatattttg tcctgccctt
      601 tagaacaaaa agcatctcca tttctttgtg acaatggtag ttttcttcca gtacatttgt
      661 aaaatccact gtaaaaggca aattgtctat aaattttata ctttcgaaaa gcatattttt
      721 tctcctcaca atcaacaata ccaagatgcg atagttttgt caaagaaaaa cagatttttc
      781 ggtggggacc gaagacaaag agaaagaaag gctatggccg gcagtgcaag cctttcttct
      841 tgttaaataa gcgcttgtag attataataa acaaagaaaa agcggttttt tataatgcaa
      901 aaaggagaaa cagttatgca gcttttcaaa cagatgaaag ttataggcat tatcatcggg
      961 gcattgattt ttattattgg agttgttttt gtggcaagac ccgtaaaggt ggagatactt
     1021 cttgactggg tactgggcat atcacttatg attggcggta taagcatgat aggcaaagtt
     1081 atagccgaga ggaacgaaaa aggtaatatt tttattagaa tgatcccggg tatcgcatta
     1141 cttgttattg cagcttttgc acttataaat gaaggcctta cagtatttat ggtggggata
     1201 ataataggaa tagcagcatt ctttatggca tttgaaagac ttactgttgc caacgaaaga
     1261 agaaaggcag ctcagtcgtg ggggaccgtg gctatcttcg gggtcataca gattttcttt
     1321 ggagcattca tgctttatgc aacattttca atgatagccg ctcttataat gataataggg
     1381 atttatttga tgataaccgg cataagcctt gttatatcat cagccatatt cccgctgccg
     1441 aaaggagaaa aatgatacct ctttccacaa ggatgaggcc ccttggactt gatgaatttg
     1501 tgggccagga acatttcatg tatcatggct cgctacttta taatgccata aaaaacaaaa
     1561 cctttgattc ggcgattttt ttcggtccct ccggaacagg caaaacgact cttgcaagaa
     1621 tagttgcaag ggaaatggat tcggactttg ttgaaataaa tgcaggagtg tcaggcacga
     1681 aagaactgaa ggaaataatc ggagcggcaa aaacgaaatt tttcggactg cagaaagaaa
     1741 agacatatct ttatgtagat gaattccaca ggtggaacaa gttgcagcag gattcgcttt
     1801 tgaaagccct ggaagaaggc gttctgagat tcataggcag cacgacacag aacccctatt
     1861 tcgccataaa caacgcggtg ctcagcagag tcaggagcat atatgaattc aggccccttt
     1921 ctgtggataa catactggta ctccttaaga ggaccctgga tgacaaagaa aggggattcg
     1981 gtaacctcag gataagttat gaagaaaaag cgctgaaaaa tctggcggat atgagtgaag
     2041 gagatgccag aatagccctt gatacccttg gattcattat agataattta aaacaagaag
     2101 ggaaaataga taacggtatg atccgggaag ccatgcagaa acgcacggtt ttttatgaca
     2161 aaagcgagga caaatataat ctgctttcgg cactgcagaa atctatcaga ggcagcgatc
     2221 ccgatgcatc catacactat cttgcaagac tcatagaagg cggagccgat gaaatgatga
     2281 tcggcaggag acttatggta atagccagtg aggacatcgg catggcctat cccaatgcca
     2341 tagctgtagt caccgcatgc gttcaggctg cccatatggt gggatttccg gaagccagga
     2401 taaatcttgc agaagctgtt atattgttgg cgtcctgtcc aaaatcgaac agagcctata
     2461 tggccctgga atctgcccaa aaagatctgg agaacaggaa aaccggagat gttccgccgg
     2521 ttctcaagga ttctcattac agcggcgcaa aaaaaagagg gataggagaa ggttacaaat
     2581 atccccatga ttacggggga tatgtaaaac aacagtatct tcccgacgaa cttcataaag
     2641 atggagtcag atattatgaa cccacagaaa acggcagcga ggcatccttc aaaaaatatc
     2701 tcgaaagttt gagggaaaag aaaaaatgac agaaataata ctttctccca attcaggatt
     2761 ttgcttcggg gtcaagctgg ctctcgaaaa agcggaagaa gccatacgga caagaaaaga
     2821 caaaaaaata tatacatgcg gacctcttat acataacagg aatgtgacag atggactgaa
     2881 ggaaaaaggt atagagatca ttgactctcc ggctgaagcg ggaaatggat cggtggtaat
     2941 agtaaggtcc catggcgagc cggaatcttt ttataaaatg gcttcagaaa gggaccttga
     3001 aatagttgat gccacatgtc cctttgtttt tcgtatacac atgcttgtca aagaagcgtg
     3061 gtcatcaggt atgcaggtag ttatagtcgg tgataaagat caccctgagg tgataggtat
     3121 aaacgggtgg tgcggaaaca gtgcatgtgt tttttcatcc ggagaagaag ctgcagaaat
     3181 ggaaagcagc aaaaaacttt ttgtggtggc acagacgact atcaccaaaa atctctttga
     3241 agaagtagta ggggaactct cttcaaaaaa cttcgaaatg gaagtcaaaa atactatttg
     3301 cagtgccaca tcagaaagac aggacgggtg tttggttacc gccaaaagtt cagatgccat
     3361 ggttataata gggggaagga gcagttccaa tacaacgaaa ttatatgaaa tagcaaaaaa
     3421 acactgtaaa aacgtttatt ttgtggaaaa tatcgacgat ttgccattga aagaaatcca
     3481 aaaatataat agaatagggg ttgcggcggg tgcatcgaca cccgaaaacg cgatcaagga
     3541 ggttatttct aacatgagtg acaacatcac tgaaaacaaa gaacttagca tggacgattt
     3601 gatggaagac atcgaaaaat ccctcagact gcccagaggc ggcgagatgg taacaggcaa
     3661 agttcatcag gttacagaca aagagatcat tgtgaatctt ggctgtaaaa aagacgggat
     3721 catcccactg gaagaaatca aactggaagg agatcagaaa ctgaccgacc tttacagtga
     3781 aggtgacgag atccaggcca aggtcctgaa gacggatgac ggcgacggaa gcatactgct
     3841 ttcaaagaaa aagttggaag ccggcgaaca ctgggagcag atcaatgatg ctcttgaaaa
     3901 taaaactttt atcgacgttg aagttttaag acaggtaaac ggaggagtga ttgctgcata
     3961 caaagaagtg cagggattca taccgctttc acagctttcg gacagattca tcgaggattc
     4021 ttcggagttc gtgggacaga cccttaccgt aagagtatca agggtggatc agagaagagg
     4081 aaaggctgta ttctcacgca aggcattcct taatgaagaa cgccagaaac tggtcgagga
     4141 aatatgggaa agcgtaaatg tcgatgatat cgtggaagga actgtaatga gatttaccga
     4201 ttacggtgct tttgtggata tcggcggaat agatggactt ttgcatatct cggagatatc
     4261 ttggggcaag ctgaagcatc ctaaggaagt gcttgaaata ggacagaaga tccacgtcaa
     4321 ggtgctttcc atgaacaacg aaaaaggcaa aatatctctt ggtctcaagc agacacaacc
     4381 cgaaccctgg agcattataa acgataaata caatgaaggc gacatcgtaa agggcaaggt
     4441 cgtacaaata aaagaatacg gatgttttgt agaacttgaa ccgggtcttg acggactggt
     4501 gcacatttcg gaagtaagcc acaggagagt gggaaaca
//
LOCUS       DAONHK010000091         4482 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_15133_length_4482_cov_3.998419, whole genome shotgun sequence.
ACCESSION   DAONHK010000091 DAONHK010000000
VERSION     DAONHK010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4482
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15133_length_4482_cov_3.998419"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(101..1021)
                     /locus_tag="PLM92_04235"
     CDS             complement(101..1021)
                     /locus_tag="PLM92_04235"
                     /inference="COORDINATES: protein motif:HMM:NF014627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HPR24377.1"
                     /translation="MKKIKVLLRDFFNRDRKEKIKMFSIDFIWVFIGCAFGAFATVSV
                     MLPNGLTSGGLTGVVRIIQNYINIDFSILYYIGSFIIIAVAGITMGFAEVKKLILISV
                     LYPAVLMFFEYTDFRLLEEKDLILAAIFCGAFSGVCSGIVFWRGYSFSGTDAIAKMIR
                     KKLMPQFSQSKIMVTIDALVITVSAFIFGRNIALYALVTQVIISKTIDVIIFGFESKI
                     IQLEIITMKGDEITEYIVNDIGRGVSRHTVRGAYTDTVRSELRLLCSPRESISVKRKI
                     AKIDPGAFVTVIKVDNVWGSGRGFGDIDKD"
     gene            complement(1023..2381)
                     /locus_tag="PLM92_04240"
     CDS             complement(1023..2381)
                     /locus_tag="PLM92_04240"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HPR24378.1"
                     /translation="MSLLSGLPGIVEAAKKEYEEAKAGDFRTTSKKGNSLSNLIVQGE
                     NFGFMKYLAEKRGLKGGIKLIYVDPPFFSGTNYQARIKTDSPLTGEVAAFKIVAYKDK
                     WAQGMDEYLKMLTSRLFMMKDLLSEDGCLWIHLDWHVVHYVKIIMDEIFGEKNFINEI
                     IWNYKSGGTSKNHFSRKHDTLLFYGKGRKTPLDIPKEKSYNRERKPYRFKGVKEYRDE
                     NGWYTMVNMKDVWHIDMVGRTSKERTGYATQKPEALLERIIGSCTKEGEICADFFCGA
                     GTAAAVAEKLGRKWIACDGGDMAVSFAEKRLAKTGASFAVMKEAVKEKKSEDRIDIKT
                     EIINTSFEGKKLIRTKITDYAPCIKSLPLEEEGVKIIKELSEKDPLSLIDYFSIDFDY
                     DRETHRSDVYYFKDRENECICCEKILEKWGKISIKITDIFGNVFMKTLDLSAGAGIFL
                     EG"
     gene            complement(2378..4126)
                     /locus_tag="PLM92_04245"
     CDS             complement(2378..4126)
                     /locus_tag="PLM92_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006309157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HPR24379.1"
                     /translation="MAFLPVSKKEIKRLGWEQPDFVIVTGDAYVDHPSFGTALIGRLL
                     ERYGYKVAVLPQPDWKTAEDFKKLGKPRLGFLINSGTIDSMVAHFSVFKKRRKKDNCT
                     PGGEAGKRPDRAVIVYSNRAREAYKGVPVIIGGLEASLRRLGHYDYWDDSVRRSVLLD
                     SKADILIYGMGERAVLEIAEALDSGINVRDITWIRGTCVRGKEEPSDEDTLILPSYEE
                     IKNHRDEYCKSFVMQYRNNDSISGKRLAEKYDEKMWVFQNPPANVLEREELDDIYDMH
                     FERQAHPVYEGKEIPALGEIKFSITSNRGCFGGCSFCALTFHQGRQVRSRSEGSLLRE
                     AVLLSERKDFKGYIHDVGGPTANFRNESCERQRRHGMCMEKECLFPRICPNLKVDHKE
                     YKELLKKMRSIKGIKKVFIRSGIRYDYVMADKDKSFLKEICLHHISGTLKVAPEHVSP
                     QVLCYMRKPSAEVFEKFAAEYKEINKNLGKKQYLIPYFISSHPGCDLNDAVTLARYLK
                     KTGFVPDQVQDFYPSPGTLSTCMYYTEKDPFTMKKIYVAKTIKEKKLQRALIHFNKKE
                     NRYLVKEALDMTGRKI"
     gene            complement(4126..>4482)
                     /locus_tag="PLM92_04250"
     CDS             complement(4126..>4482)
                     /locus_tag="PLM92_04250"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HPR24380.1"
                     /translation="VCYDVRFPELFIDMAKKGAKAVVLPAAFSKKTGEAHWDITMKAR
                     AVDSQIYMAAVSPARDEKGPYVCYGYSKIIDPWGKEIAAAKTEETLITAEIDTEYVDD
                     VRRQLPLLKHRKEGLY"
BASE COUNT         1123 a         1077 c          776 g         1506 t
ORIGIN      
        1 ataaccgcga aataaatccg ggaaaaaaag actgatctgc tgacatataa cagaaaacga
       61 aataaaaaaa gagcggttgt ctgtccgctc tttgacttta tcagtctttg tctatgtcgc
      121 cgaaccctct tcctgagccc cagacattgt ccacctttat gacagtcaca aatgctcccg
      181 gatctatctt tgcgattttt ctttttacgc ttatgctttc ccggggcgag caaagcagcc
      241 gcaattcgct ccttactgta tccgtatatg ctcctcttac agtatgtctt gagacacctc
      301 ttcctatatc attgacaatg tattcggtta tctcgtcccc cttcatggtt attatttcaa
      361 gctgaattat ttttgactca aatccgaata ttatcacatc tatagtcttt gatattatca
      421 cctgggtcac aagtgcataa agggctatgt tccttccgaa tataaaggcg gaaactgtta
      481 ttaccagggc atcaatggta accattattt tgctctgcga aaactgaggc ataagttttt
      541 ttcttatcat tttggctatg gcatccgttc cgctgaatga atagcctctc cagaatacta
      601 tgccgctgca gacacctgaa aaagccccgc aaaatatggc ggccagtata agatctttct
      661 cttccagaag acgaaaatcc gtatactcaa aaaacataag gaccgcagga taaagtacac
      721 taataagaat aagctttttt acttctgcaa atcccattgt aatgcccgct actgctataa
      781 ttatgaaaga gcctatataa tatagtatgg aaaaatctat gtttatatag ttttgtatta
      841 ttcttaccac tcctgtcagc cccccggaag tcaatccgtt gggtaacatg acagaaactg
      901 tggcaaaagc accaaaagca catcctataa aaacccaaat aaaatcgata gaaaacatct
      961 ttatcttttc tttacggtcc cgattgaaga aatctcttaa taaaactttg atctttttca
     1021 ttttaacctt ctaaaaatat tccggcaccg gcagacagat ccagtgtctt catgaaaacg
     1081 ttcccgaata tatccgttat ctttatactg attttgcccc atttttccag tattttttca
     1141 cagcatatac attcgttttc tctgtctttg aagtaatata catctgacct gtgtgtttct
     1201 ctgtcatagt caaaatctat actgaaatag tctatgaggg aaagggggtc cttctctgac
     1261 aattctttta ttatctttac cccctcttct tccaggggaa ggctttttat gcagggggca
     1321 tagtctgtta ttttcgttct tatcagcttt ttcccctcaa aagatgtatt tatgatttca
     1381 gtttttatat ctattcggtc ttcgcttttt ttctctttta cagcttcttt cattacagca
     1441 aaagatgctc cggtctttgc cagtcttttt tctgcaaagg aaacagccat gtctccaccg
     1501 tcacatgcta tccattttct tccaagcttt tcggcaacag cagctgctgt acctgctccg
     1561 cagaaaaaat cagcacatat ttctccttct tttgtgcaac ttccgattat tctttcaagc
     1621 aatgcttcgg gcttctgagt ggcataccct gtcctttcct ttgatgtacg gccaaccata
     1681 tctatatgcc agacatcttt catattgacc attgtatacc agccgttttc gtcccggtat
     1741 tcttttactc ctttgaatct gtaaggcttg cgttctctgt tatatgactt ttcttttggt
     1801 atgtcaagag gcgttttcct tcctttgcca taaaacaaca gtgtatcatg cttgcgcgaa
     1861 aaatgatttt tgctggtgcc tccggatttg taattccaga tgatttcatt aatgaagttt
     1921 ttttcaccaa agatctcgtc cattattatt tttacataat gtaccacatg ccagtcaagg
     1981 tggatccata agcatccgtc ttctgacaaa agatccttca tcataaaaag tcttgatgtc
     2041 agcatcttta aatattcatc cattccctgc gcccatttgt ctttataggc cactatcttg
     2101 aatgccgcca cttctcctgt caaaggtgag tcggttttta tccttgcctg ataattggta
     2161 ccggagaaga aggggggatc cacatatatc agttttattc ctcccttaag tcctctcttt
     2221 tcagcaagat acttcatgaa accgaaattt tctccctgaa ctataagatt tgaaagagag
     2281 ttgccttttt tgcttgttgt tctgaaatct cctgcttttg cttcttcata ttcttttttc
     2341 gctgcctcca ctataccggg cagtccgctg agcaagctca tatcttcctc cctgtcatat
     2401 caagggcttc cttcacaaga tatctgttct cttttttgtt gaagtggatc agtgcccgct
     2461 gaagtttttt ttctttaatg gtttttgcca catatatttt tttcatggta aaagggtctt
     2521 tttccgtata gtacatgcat gtcgaaaggg tcccgggaga cggataaaaa tcctggacct
     2581 gatccggaac gaaaccggtt tttttgagat accgggcaag agtaaccgca tcgttaagat
     2641 cgcatcccgg atgcgatgaa ataaaatacg gtatcagata ttgtttttta cccagatttt
     2701 tgtttatctc tttgtactcg gcagcaaatt tctcgaagac ctctgcagaa ggttttctca
     2761 tataacagag tacttgaggt gaaacatgtt ccggcgcaac tttcaaagtg ccgcttatat
     2821 gatgcagaca aatttcttta aggaaacttt tgtccttgtc tgccataaca taatcatacc
     2881 ttattccgga tcgtatgaaa acttttttta ttcctttgat cgatctcatc tttttgagaa
     2941 gctctttata ttctttatgg tccaccttta gattgggaca gatccgggga aaaagacatt
     3001 ctttttccat gcacatacca tgcctccttt gcctttcgca tgattcgttt ctgaaatttg
     3061 ccgtagggcc ccccacatca tggatatagc ccttaaaatc tttcctttca gacaatagca
     3121 ctgcctctct taaaagagac ccttcgctgc ggctccttac ctgtctgccc tgatgaaaag
     3181 tcaatgcaca aaagctgcat cctccgaaac aacctctgtt ggaagttatg ctgaatttta
     3241 tttctcccag tgcaggtatt tctttccctt catatacagg atgtgcctgt ctctcaaaat
     3301 gcatatcata aatatcatcc agttcttctc tttcaagaac gtttgcaggg gggttctgaa
     3361 atacccacat tttttcatcg tatttttccg ccagtctttt gcctgatatg ctgtcattgt
     3421 ttcgatactg cataacaaag cttttgcagt attcatccct gtgatttttt atttcttcgt
     3481 aagaaggcag gatcaacgta tcttcgtctg atggttcctc cttccctctt acgcaagttc
     3541 cccttatcca tgttatgtca cgaacgttga tgcccgaatc cagagcttct gcgatctcca
     3601 ggactgccct ttcccccatc ccgtaaataa gaatgtccgc cttgctgtcc aaaagcacag
     3661 atcttcttac ggaatcatcc cagtaatcat aatgtccaag tctcctcaaa ctggcttcaa
     3721 gtccgcctat tatgacaggg actcccttat aggcttccct tgctctgttg ctgtatacta
     3781 tcactgccct gtccggtctt ttgccggctt caccacccgg tgtacagtta tccttttttc
     3841 tccttttttt gaaaacagaa aaatgagcaa ccatggaatc gatggttccc gaatttataa
     3901 ggaatccgag ccttggcttt ccgagcttct taaaatcctc tgctgttttc caatccggtt
     3961 gaggcagcac agcgacttta tatccatagc gctcaaggag tcttccgata agagctgtgc
     4021 cgaaggaggg atgatccaca taggcatcac cggtcacaat aacaaaatcc ggctgttccc
     4081 agccaagcct ttttatttct ttttttgaaa cgggcaaaaa agccatcagt ataatccctc
     4141 tttcctatgt ttcagtaaag ggagctgcct tctgacatca tccacatact ccgtgtctat
     4201 ttctgctgtt atcagcgttt cttcagtttt tgcagctgct atttctttcc cccagggatc
     4261 tattatcttt gagtatccgt aacaaacata tggccctttt tcatcccgtg ccggagaaac
     4321 ggcagccata tatatctggc tgtctacagc tctggccttc atagtaatgt cccagtgggc
     4381 ctccccggtc tttttgctga atgccgcagg aagaaccact gcttttgccc ctttttttgc
     4441 catatcaata aagagttccg ggaaacggac atcatagcag ac
//
LOCUS       DAONHK010000092         4364 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_15732_length_4364_cov_4.902298, whole genome shotgun sequence.
ACCESSION   DAONHK010000092 DAONHK010000000
VERSION     DAONHK010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4364
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15732_length_4364_cov_4.902298"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1714)
                     /locus_tag="PLM92_04255"
     CDS             complement(<1..1714)
                     /locus_tag="PLM92_04255"
                     /inference="COORDINATES: protein motif:HMM:NF016450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPR24381.1"
                     /translation="MPKAVKLGKMERQTFSKINEVAEMPNLIQIQTKSYDWFVKEGLR
                     EVFEDISPIEDYAGNLVLEFTDYSLDEPPKYSQEKCRERDVTYATPLKVKVRLITNET
                     GEVKEQEVFMGDFPLMTEKGTFIYNGAERVVVTQLVRSPGPYYSQEIDKSGKELFSTQ
                     VIPNRGAWLEYETDSNEILSVRVDRTRKQPITALIRALSQEGIKQKIKNVIEQKYREK
                     YKPEKGWTFDNFTQEEIKNLEVEFKDELKKKVRRYQELNSIVSKKLVSGTDEELIEIF
                     GEDERLIKSLEKDPSTNYDDALKEIYKKLRPGEPPTFDSAYDIIESLFFDPKRYDIAK
                     VGRHKYNKKLGLSNRIEKVMAAADVVDPNTGEILAEKDEVISTEMAVRIENAGVQEVI
                     AYGKDNKDVTTKIIGNNFVELKEYIKFDISDIKIPEKVYYPVLMDILKECQEDDDIKQ
                     ALISRKNELSPKRIILDDIIASISYLLNLRFGVGNKDDIDHLGNRRLRTVGELLQNQF
                     RIGLARMERVVKERMTIQDIEVTTPQALMNIRPVTAAIKEFFGSSQLSQFMDQTNPLA
                     ELTHK"
     gene            complement(1904..2272)
                     /gene="rplL"
                     /locus_tag="PLM92_04260"
     CDS             complement(1904..2272)
                     /gene="rplL"
                     /locus_tag="PLM92_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HPR24382.1"
                     /translation="MTKAEIIEAIKNMSVMELNELVEACEEEFGVSAAAPVAVAGGNG
                     AAAGAVEEQTEFTVVLKEVGAEKIKVIKVVREITGLGLKEAKDIVDKAPSNIKEGIEK
                     AEAEQIKNQLEEVGAGVELQ"
     gene            complement(2323..2985)
                     /gene="rplJ"
                     /locus_tag="PLM92_04265"
     CDS             complement(2323..2985)
                     /gene="rplJ"
                     /locus_tag="PLM92_04265"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HPR24383.1"
                     /translation="MNDTIKAAYAQKQVVIDEIKDKFEKAQSAVVVDYIGITVAQADK
                     MRKNLREADVDYTIYKNTLVKRAIEGTAYQKLEEVLTGPSAFAISYDDVTSPARLIND
                     AIKEFKKMSFKGGIIEGTYYDEEGLKKIALIPSKDELIAKFMGSIQSPVGKAVRTFAA
                     IAEAKTEGADDSSDTEKKEEKAARPAQETKEAEPVKEETEPAKEAAEPVKEEAEPAKE
                     EA"
     misc_feature    complement(3040..3155)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(3169..3870)
                     /gene="rplA"
                     /locus_tag="PLM92_04270"
     CDS             complement(3169..3870)
                     /gene="rplA"
                     /locus_tag="PLM92_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HPR24384.1"
                     /translation="MPKRSRNYKENAEKIEKANLYEPKEAVTLAAETAKAKFDETIEV
                     HIKLGVDGRHADQQVRGALVLPHGTGKSKKVAVFAKGAKVKEAEDAGADYVGAEDLAQ
                     RIQSENWFDFDVVVATPDMMGVVGKLGKVLGPKGLMPNPKSGTVTMDLGKALSDIKAG
                     KVEYRLDKSNIIHTTIGKASFGAEKLNDNFMALISAVIKAKPSAAKGQYLKSVTVAAT
                     MGPGIKINTAKLTAN"
     gene            complement(3915..4340)
                     /gene="rplK"
                     /locus_tag="PLM92_04275"
     CDS             complement(3915..4340)
                     /gene="rplK"
                     /locus_tag="PLM92_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016282999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HPR24385.1"
                     /translation="MAKKIDGYIKLQIPAGGATPAPPVGPALGQKSVNIMDFCKQFNA
                     KTQDQQGMIIPVVITVYADRSFSFICKTPPAAVLLKKAAGLESASGEPNKTKVATLKR
                     EQCEEIAKLKMPDLNAADLESATEMIKGTARSMGIVVED"
BASE COUNT          854 a         1058 c          811 g         1641 t
ORIGIN      
        1 tcttatgcgt caactccgcc agagggtttg tctgatccat aaactgtgaa agctgtgagc
       61 tcccgaaaaa ctcttttatt gcagcagtaa caggccttat gttcataaga gcctggggcg
      121 ttgtgacttc gatatcctgt atcgtcattc tttcctttac tactctttcc attctggcaa
      181 gccctatcct gaactggttc tgcaggagtt cccccacagt cctcagtctt ctgttgccca
      241 gatggtctat atcgtctttg ttccccactc cgaatctgag attcagaagg taacttatcg
      301 atgcgattat atcgtcaagg attattctct ttggtgaaag ttcatttttt cttgatatta
      361 aagcttgctt tatatcgtca tcttcctgac actcttttaa gatgtccatg aggaccggat
      421 agtaaacttt ttcgggtatc tttatatcag atatatcaaa ttttatgtac tctttaagct
      481 ctacgaaatt gttaccaatg atcttggttg ttacatcttt attatccttt ccataggcta
      541 ttacttcctg aacaccggca ttttctattc tgacggccat ttctgtactt atgacttcat
      601 ctttttctgc cagtatctca ccggtgttgg gatcaacaac gtccgctgct gccatcacct
      661 tctctatcct gttggaaagg cccagttttt tgttgtactt atgtctgccc acctttgcga
      721 tatcatatct cttggggtca aaaaacaatg attctattat gtcataggcg ctgtcaaatg
      781 tggggggttc tcccggtctc aatttcttgt atatctcttt taatgcatca tcataattgg
      841 tggacggatc cttttcaaga gatttgatga gtctttcgtc ttccccgaat atttctatga
      901 gctcttcgtc tgtgccgctt accagcttct tcgaaacaat gctgttcagt tcttggtatc
      961 tgcgtacttt tttcttcagc tcatctttga attctacttc aagattcttt atctcttctt
     1021 gagtaaagtt atcaaaagtc cagccttttt cgggcttgta tttttctctg tatttctgct
     1081 cgataacatt ctttattttc tgctttatgc cttcctggga caaagctctg ataagtgcag
     1141 ttatcggttg tttccttgtt ctgtcaactc ttactgaaag tatctcgttt gagtcggttt
     1201 catattccag ccatgctcct ctgttgggaa tgacctgtgt cgaaaagagt tcttttccgg
     1261 atttgtcgat ttcctggcta tagtaaggtc cgggagaacg aaccagctgg gttacaacga
     1321 ctctttcggc gccgttatag atgaatgtgc ctttttctgt cataagaggg aagtctccca
     1381 taaaaacttc ctgttctttt acttctcctg tttcatttgt tatgagcctc acctttacct
     1441 tcagcggtgt ggcataggtc acatctcttt ccctgcattt ttcctgggaa tactttgggg
     1501 gttcatcgag ggagtaatcg gtaaattcca gtactaagtt tcctgcataa tcctcaatag
     1561 gagaaatgtc ttcaaacact tctctcaagc cttcttttac aaaccaatca tatgatttgg
     1621 tctggatctg aatcaggttg ggcatttcgg caacttcgtt gatttttgaa aatgtctgac
     1681 gctccatttt acctaatttt acggcttttg gcatattcat cactccttgt atttttattt
     1741 aatcatatta cgtgtcagcc tattattata catcgtatgt gtcaattttt caagtataat
     1801 ttgttttttt tgcagctttt caaaaatgca caatagctga gcccttttag ctgagctcag
     1861 ccactatgat tttattatct tttatgagct tttattatat gcattactgc aattctacgc
     1921 ctgcgcccac ttcttccagc tgattcttga tctgttcagc ttctgccttt tctatacctt
     1981 ccttgatgtt ggagggagct ttgtctacaa tgtcttttgc ttctttaagt ccaaggcctg
     2041 ttatttctct taccaccttg atcaccttga ttttttcagc tccgacttct ttaagtacta
     2101 ctgtgaactc agtctgctct tcaacagctc ctgccgctgc cccgtttccg cctgccactg
     2161 ccacgggggc tgctgcggat acaccgaatt cttcttcgca tgcttcaacc agttcgttca
     2221 gttccataac tgacatattt tttatggctt caattatctc tgctttagtc attttattat
     2281 ctccttttct tttacttttt atgttttctt ttcttttgaa gtttaagctt cttcctttgc
     2341 aggttcagcc tcttccttca ccggctctgc tgcttccttt gcgggttccg tttcttcctt
     2401 caccggctct gcttcctttg tctcttgagc cggccgggct gctttttctt ctttcttttc
     2461 tgtatcggac gagtcatcgg ctccttcagt ttttgcttca gcaatggccg caaatgtgcg
     2521 gactgccttg cctaccggtg actggatgct tcccatgaat tttgctatca gttcatcctt
     2581 tgacgggatc agggctattt tcttaaggcc ttcctcatca tagtaagttc cctctatgat
     2641 accgcctttg aaagacattt ttttgaattc ttttatggca tcatttatta atcttgccgg
     2701 tgatgttaca tcatcatagc ttatggcaaa ggcgctgggt cctgtcagca cttcttccag
     2761 tttctgatag gcagtccctt ctattgccct tttgacaagg gtgttcttgt aaatggtata
     2821 atcgacatcc gcttcacgaa gattttttct cattttgtca gcctgcgcca ctgttattcc
     2881 tatatagtca acaactactg ccgactgggc tttttcaaat ttatctttga tttcgtctat
     2941 aacaacctgc ttctgagcat aagctgcttt tattgtgtca ttcatttctc tgagttctcc
     3001 tttccggcct ttttcatttg ctgtcaggcc ttttttacgg tactccataa aaaaccttgt
     3061 ttgccacaga caaacaaggt caatatttta tattgtacct cggcgggaga ttaagctttc
     3121 gcacccgctg tctacggttt cgttttctta agttttgtta cggctctttt agttggctgt
     3181 cagtttagcc gtattgatct tgatcccggg gcccatggtt gcggccacgg tcacactttt
     3241 gagatactgt ccctttgctg ccgaaggttt tgccttaata acggcactta taagtgccat
     3301 aaagttgtca ttaagttttt cggctccgaa ggaagctttt cctatggtcg tgtgaattat
     3361 attgctcttg tcaagacggt attcaacttt acccgctttt atgtcagaaa gagcttttcc
     3421 gagatccatg gttacggtcc ctgatttcgg gttaggcata agtccctttg gtcccagcac
     3481 tttacccagt tttcccacaa ctcccatcat atcgggagtg gcgacaacaa catcgaaatc
     3541 aaaccagttt tctgattgta tcctctgtgc caggtcttct gctcccacat agtccgctcc
     3601 tgcatcttcg gcctctttta ctttggcacc ttttgcaaaa actgcaactt ttttggattt
     3661 gcctgtgccg tgaggaagta caagggctcc tcttacctgc tgatctgcgt gtcttccgtc
     3721 gacacccagt ttgatatgca cttcgatagt ctcatcaaat ttcgcttttg ctgtttccgc
     3781 tgccagcgta acagcttctt ttggttcgta aagatttgct ttttctatct tttctgcgtt
     3841 ttctttgtaa tttctgcttc ttttaggcat tttttcctcc ttgtggtgct atcggctcaa
     3901 agtcctccca ctttctaatc ttctactact atgcccatgc tccttgcggt ccctttgatc
     3961 atctctgttg ccgactccag gtctgccgca ttaagatcgg gcattttcag ttttgcgatc
     4021 tcctcgcact gttctctttt gagtgttgcc actttggttt tgttgggctc tccggaagcc
     4081 gattccagtc ctgctgcttt tttcagcaac actgctgcag gtggcgtttt gcatatgaat
     4141 gaaaacgatc tgtctgcgta cactgttatc actaccggga ttatcattcc ctgctgatcc
     4201 tgtgtcttgg cgttgaactg tttgcagaaa tccattatat taacactttt ctgccccagt
     4261 gccggtccta caggtggtgc aggcgttgct ccgccggccg gtatctgcag tttgatatag
     4321 ccatcgatct tcttagccat gcttttcctc ctttcctcat atta
//
LOCUS       DAONHK010000093         4348 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_15813_length_4348_cov_4.548334, whole genome shotgun sequence.
ACCESSION   DAONHK010000093 DAONHK010000000
VERSION     DAONHK010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4348
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15813_length_4348_cov_4.548334"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /gene="zapA"
                     /locus_tag="PLM92_04280"
     CDS             complement(<1..470)
                     /gene="zapA"
                     /locus_tag="PLM92_04280"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HPR24386.1"
                     /translation="MGSNKVSVRIYGQEYIISGDKSQEHMIRVAAYVDTKMHELGSLF
                     KKSPVSSLAVLSAVNIADEYFTVMQKIDESKITNEQMEGDIHRYEQLWEEAKKTLSDQ
                     RKETQGVIKEKEKLMEKFREKEKQIEELLRGEKNIEAEVEKHKAMAEAETEEKY"
     gene            complement(486..2918)
                     /gene="pheT"
                     /locus_tag="PLM92_04285"
     CDS             complement(486..2918)
                     /gene="pheT"
                     /locus_tag="PLM92_04285"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HPR24387.1"
                     /translation="MLVPIEWLKDYTDVNVDTEEFCEKMIMSGSNIESVEYFGEGIEN
                     VVVGKVIKKEKHPDADKLSVCMVDVGEDAPLQIVCGAANVAEGINVAVALDNSRIPGP
                     LHGQSEKEGGTRIKKGKLRGVLSYGMICSAQELGFDDKVIPVAHKDGIWILPEGLSPG
                     ADISDAMELVSAVVDFEITPNRSDCLSMIGMARESAATLKSKLHYPEIKSSSASDKEA
                     KDFISISIKDPDLCKRYAAKIVTDIRIQQSPWWLQKRLMYAGMRPINNIVDITNFVML
                     EYGQPIHAFDIRQIEGKKIVVETASKGEKFVTLDENERTLDESMLMIKDGMKNLAVAG
                     VMGGLDSEIKNDTNTILVESANFDPESIRRTSKKLGLRTEASSRFEKKVDPALCRTCA
                     ERVCSLIEMLGAGTVVRGEVDVYPVPESPKKIDVRPARINHVLGVEIAADEMAEMFEA
                     LEMEVEREGELLKVIPPSVRRDLDSEVDFTEEAARMYGYDRLPVTIPKGNCEAVLSRE
                     ENLRKLTRDTLCALGANEIQTYSFVSPKSVDHIGIEEDSWERNFVYLKNPLGEENSVM
                     RTILTPGMLEVMGRNWSRKAESLKCFEIGSTFGKDLIDNEALPEEQDALCIGLYGEGY
                     DFFTLKGMVSEMLNVLKIKDPEFEAENGYRVYHPGRCARISYKGEELGIMGEIHPDVA
                     ELMGIGKRCYVAEFMFDKVIKNASTEVYYSPLPKYPAVARDIALLIKEDIPAGSVEKV
                     ICEAGGNILESVKLFDVYRGKQVDSGMKSAAFALTYRHPEKTLTDEEVAKIHEKVLAA
                     LKEKFDAVLRDV"
     gene            complement(2930..3952)
                     /gene="pheS"
                     /locus_tag="PLM92_04290"
     CDS             complement(2930..3952)
                     /gene="pheS"
                     /locus_tag="PLM92_04290"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005999681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HPR24388.1"
                     /translation="MQEKLKSILENTRNLLEKAAGIDETEMIRVKVLGKKGELTEILK
                     SMGKLAPEERKELGRAANDVRKEIEKLLEEKFAKVKEAEKESRFGLEKIDVTEPGREF
                     VLGVKHPLTIVTEEISGIFMSMGYSYAEGPQADTVFNAFDALNSPKDHPSRDITDTFY
                     ISDDVVLRPHTSTVQIRTLIKQEPPIKIISPGRCFRCDTFDATHSPMFQQIEGLLVDE
                     GINMADLKGTLDSMAKQLFGSATKTKFRPHYFPFTEPSAEMDVSCFKCGGKGCNICKG
                     SGWIEILGCGMVHPNVLAVGGLDTEKYTGFAFGMGVERIAMLKYGVDDIRLFYENDMR
                     FINQFK"
     gene            complement(3966..>4348)
                     /locus_tag="PLM92_04295"
     CDS             complement(3966..>4348)
                     /locus_tag="PLM92_04295"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HPR24389.1"
                     /translation="RTAEAAGYGGAIIMKGSGDIYSPKAVRAAAGSILRFPVMLTEEA
                     NDTAEVLLKNGKRIISTKADAENDFRETDLTGNTALVIGNEGKGVSDEFISLSDETLR
                     IPMKGEVDSLNAAVAAGILMYSSI"
BASE COUNT          964 a         1130 c          778 g         1476 t
ORIGIN      
        1 ttgtatttct cttctgtttc ggcttcggcc atggctttat gcttttctac ttcagcctcg
       61 atattctttt ctcctctgag aagttcttct atctgttttt ccttttctct gaatttctcc
      121 ataagctttt ctttttcttt tatgaccccc tgtgtctctt ttctctgatc cgaaagcgtt
      181 tttttggctt cttcccaaag ctgctcgtac ctgtggatgt ctccttccat ctgttcattt
      241 gttatctttg attcatcgat cttctgcata acagtaaaat actcgtcagc aatattaacc
      301 gcagaaagca cagccagaga agatacgggg ctttttttaa acagactgcc gagttcatgc
      361 attttagtgt ccacatatgc ggccactctt atcatgtgtt cctgtgattt gtctcctgag
      421 ataatgtatt cctgtccgta gatccttacg cttactttgt tgcttcccat ttctcctcct
      481 cctgtttata catctcttaa gacggcatcg aatttttctt ttagcgccgc aagaactttt
      541 tcgtgtattt tggccacttc ttcgtcggtg agtgtttttt cgggatgtct gtatgtgaga
      601 gcaaaagctg cgctcttcat accgctatca acctgttttc ccctgtaaac atcaaatagt
      661 tttacgctct ccagtatgtt tccgcccgct tcgcagatca ctttctcgac agagccggcg
      721 ggtatgtctt cttttataag caaagctatg tccctggcaa ctgcgggata ttttggaaga
      781 gggctgtaat aaacttccgt gctggcattt tttatcacct tatcgaacat gaactcagca
      841 acataacatc tctttcctat ccccataagc tccgccacat cgggatgtat ctctcccatt
      901 atgcccagtt cttcgccttt ataggatatc ctggcacatc tgcccggatg atataccctg
      961 tagccgtttt cggcttcaaa ttccgggtct tttattttta gtacattcaa catttcactg
     1021 accatgccct tcaaagtaaa gaagtcatat ccttcaccgt aaagacctat gcaaagtgca
     1081 tcctgctctt cgggaagagc ctcattatcg atcagatctt ttccgaacgt actgcctatc
     1141 tcaaagcatt ttaatgattc tgcctttctt gaccagtttc tccccattac ttcaagcatg
     1201 ccgggagtaa gtatagtacg cattacggaa ttttcttctc caagaggatt tttcagataa
     1261 acaaaatttc tttcccagga atcttcttct atacctatat gatcaacact tttggggctt
     1321 acaaaagaat atgtctgtat ttcatttgca cccagagcac aaagtgtatc tcttgtcagc
     1381 tttctgagat tttcttctct gcttaagacg gcttcacagt ttcctttggg tatagtcaca
     1441 ggaagcctat catatccgta catccttgct gcttcttcgg tgaagtccac ctccgaatca
     1501 agatctcttc tcacagaagg cggtataact ttaagaagct ccccttctct ttcaacttcc
     1561 atttccaggg cttcaaacat ttctgccatc tcatcagctg ctatttctac acccagcaca
     1621 tgatttattc ttgcaggtct tacatctatc tttttcgggc tttccggaac cggataaaca
     1681 tccacctcac ctctgacaac ggtgcctgct ccaagcattt caataagact gcagactctc
     1741 tctgcacagg tcctgcaaag agcagggtca actttctttt caaatctgga agaagcctcg
     1801 gtacgaagac ccagtttttt tgatgtcctt ctgatgctct ccggatcgaa gttggcggat
     1861 tccaccagga tggtatttgt atcattcttg atctccgaat caagacctcc catgacacct
     1921 gccacagcca aattcttcat tccgtccttt atcatcagca tggactcatc aagagtcctt
     1981 tcgttttcgt ccaaagtaac gaatttctca ccctttgaag ctgtttccac aacgattttt
     2041 tttccttcta tctgtcttat atcgaatgca tgtatgggct ggccgtattc cagcataaca
     2101 aaattggtta tatcaacaat attgtttatt ggcctcatgc ctgcatacat cagtcttttc
     2161 tggagccacc agggggactg ctgtatcctg atatcggtaa ctatctttgc cgcatatctt
     2221 ttgcacaggt cagggtcttt tatgctgatt gaaataaagt cttttgcctc tttatcggat
     2281 gcggatgagc tctttatttc agggtaatgc agtttgcttt ttagtgtagc cgccgattct
     2341 cttgccatgc ctatcatgga aagacagtcg gatctgttgg gggttatttc aaaatccact
     2401 acagcactta caagttccat ggcatcgctg atatctgccc cgggcgaaag tccttcaggc
     2461 agtatccata tgccgtcttt gtgagccacc ggtatgacct tgtcatcaaa acccagttcc
     2521 tgggcagagc atatcattcc ataggataag actcctctaa gttttccttt ttttattctt
     2581 gtgccaccct ctttttcgga ctgcccgtgc agggggccgg gtatcctgct gttatccaat
     2641 gcaactgcaa cattgattcc ctctgccaca tttgctgcac cgcatactat ctgcaacggg
     2701 gcatcttctc caacatccac catgcataca gaaagcttgt ccgcatcggg atgcttttct
     2761 ttttttataa cttttccgac tacaacgttt tcgatacctt ctccaaagta ttctacgctt
     2821 tcaatattgg agccggacat tatcattttc tcacagaatt cttccgtgtc cacattcaca
     2881 tctgtataat cttttagcca ttcaattggt actaacattg acaaacctcc tatttgaact
     2941 ggttgatgaa acgcatatcg ttctcataga acagccttat atcatcaact ccgtatttca
     3001 gcatcgcgat cctctctaca cccattccga aggcaaaccc tgtatacttt tctgtatcaa
     3061 gaccgcccac ggccaaaacg ttggggtgaa ccatgccgca tcccaggatc tctatccatc
     3121 cgcttccttt gcagatattg catcctttgc ctccgcactt gaagcatgat acgtccattt
     3181 cggcactggg ttctgtaaag gggaaatagt gaggtctgaa ttttgttttt gtggcagaac
     3241 caaagagctg ttttgccatg ctgtcaaggg tgcctttgag gtcggccatg tttattccct
     3301 catccacaag aagcccttct atctgttgga acatgggtga atgggtggca tcaaaagtgt
     3361 cgcatctgaa gcatctgccc ggtgagatta tttttatcgg gggttcctgt tttatcaatg
     3421 tccttatctg tactgtggaa gtatgaggcc tcaataccac atcatcggat atataaaatg
     3481 tatctgtgat atcccttgac ggatgatcct taggcgaatt caatgcatca aaggcattaa
     3541 aaaccgtatc cgcctgaggg ccttccgcat atgagtagcc catggacata aaaattcccg
     3601 atatctcttc tgttactatg gtcaggggat gtttcacccc caggacaaac tccctgcccg
     3661 gctctgtaac gtctattttt tcaagcccga atctgctttc tttttccgct tccttcactt
     3721 tggcaaactt ttcttccaga agcttctcta tttcttttct cacatcgttt gccgccctgc
     3781 ccagctcttt tctttcttcc ggcgccagct tgcccatgga tttcaatatc tccgtgagtt
     3841 cgcctttttt acccagcact tttactctga tcatttctgt ttcatcgatg cctgctgcct
     3901 tttcaagcag atttcttgtg ttttcaagta tactttttaa tttttcctgc attctcttaa
     3961 cctttctata tggaactata cataagtatg cccgcagcca ctgctgcatt gagagaatca
     4021 acttctccct tcataggtat tctgagtgtt tcatctgaca aacttatgaa ttcatctgat
     4081 actccttttc cttcattccc gatcaccagg gcggtattcc cggtcagatc cgtttctctg
     4141 aaatcatttt ctgcgtctgc ttttgtgctg atgattctct tgccgttttt caaaagaacc
     4201 tctgcggtat cgttcgcttc ttccgtcaac atcacaggaa atcttaatat ggaaccggct
     4261 gcagctctta ctgcctttgg agaatatata tctccgctac ccttcattat gattgcgcct
     4321 ccgtatccgg ccgcctcagc tgtcctta
//
LOCUS       DAONHK010000094         4334 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_15887_length_4334_cov_5.065670, whole genome shotgun sequence.
ACCESSION   DAONHK010000094 DAONHK010000000
VERSION     DAONHK010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4334
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15887_length_4334_cov_5.065670"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(75..1394)
                     /locus_tag="PLM92_04300"
     CDS             complement(75..1394)
                     /locus_tag="PLM92_04300"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HPR24390.1"
                     /translation="MNKKNNSKKTFFSMPILNAITAVAITVTVFFSGFSAVIAETEDI
                     PVSEKPMGKINARSAVLMDAKSGNILFEKEADKRLYPASCTKILSVIVVIENADLTDI
                     VTVSNNAVMKGISHRGAHIALRPGEKITVEDALYGALLVSANDCIIALGEHVAGSEKA
                     FVEMMNKKAAELEMNDSHFANTCGLFHKEHYSSARDLAIAMNYAIKNETFVKILGTSK
                     HVIPKSNKCDEKRYLYNNHRMIRFHSFEYEGVVGGKKGYITESKFNLVTYVKRGDMDL
                     IAVVMKGSGSDKNCKDTIKMFDHYYKGYKAMALSKKNINAALGKDGTFGVLGKKAEVS
                     WERPFKVIVPKSTTKKQLFSGIKITATEEAGLCFPVKKHSTVGKLTAEYNGKTVGYAC
                     LFSKEKVESNTAIFKKVLKITIPVLMILTVISLFFLRRRASQRNIEK"
     gene            1561..2337
                     /locus_tag="PLM92_04305"
     CDS             1561..2337
                     /locus_tag="PLM92_04305"
                     /inference="COORDINATES: protein motif:HMM:NF025865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPR24391.1"
                     /translation="MDMKKAIEMRRSKRKYIDIPLDEEMISMLRKSMETYNREGGVDI
                     RMVLGDEGAFGTAIGGIKKSYGLFSGVKNYFILISKLSREEHFDPLMYEKLGYYGEKL
                     VLEATAMGLGTCWVGGTYDKEAVVCSLDKDEEIASLIAFGATADKIPISEQIVGKALH
                     YRRSKEVKDMLRTSGDVENWIIEGMRCVVKAPSSINMQPVIFTASGDMVSAKVKGKRA
                     MEFFDLGIAKLHFEIGAGGGRWDFGNGGVFQPPPERHQDR"
     gene            complement(2418..2555)
                     /locus_tag="PLM92_04310"
                     /pseudo
     CDS             complement(2418..2555)
                     /locus_tag="PLM92_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007487901.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
     gene            complement(2724..3965)
                     /locus_tag="PLM92_04315"
     CDS             complement(2724..3965)
                     /locus_tag="PLM92_04315"
                     /inference="COORDINATES: protein motif:HMM:NF015400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HPR24392.1"
                     /translation="MKKILVLGTGAQGSTVAQRLDEEPNVSEIICADYDKKAVDSLVG
                     ILKKGKAAKVDASKKEEIMKLAAGTDLIVNALPLDYGINVMETAIEVKADYQDFAACE
                     NVAHCEDPADEWVEGIKIMYKDHGERFKKAGKTALIGTGSAPGLICVMARKTIRELDT
                     CDTINMMVYEGVESKRFLPFWWSPLVALSDMSEEAYAFENGRIIRTQPFSKPLTRQFP
                     EMGGESVTMVEHAHDEPVYMGLNSEKFFKGVKNIYFKYGGVGIDFARPLYRAGLLSRE
                     EEEIKGRKVIPFDVVLAHVPPAPKYHEEVKDIIEEGLVSDTGAMVIEAYGKKDGKNVM
                     VDSHIFAPGLVDSFERSGLSAEMYLTGQGGFLFSKMFVNDKIKQKGLISSDMLTDEQV
                     DYYIECAAKLDITMETEIVPL"
BASE COUNT         1211 a          879 c          880 g         1364 t
ORIGIN      
        1 agccagggct tatagcccta gagcaaacca ccctttggaa gggtggttat gattgtgcgt
       61 tttttattca taatttactt ttctatgttt cgctgtgaag ccctgcgcct taaaaagaaa
      121 agagatatca ctgtcaggat catcaaaaca ggtatggtga tttttaacac ctttttgaat
      181 attgcagtat tggattcgac tttttccttt gagaaaagac atgcatagcc cacggttttt
      241 ccgttgtact cggcagtcag tttgcctacg gtcgaatgtt ttttcaccgg gaaacaaagt
      301 ccagcctctt ctgttgcggt tatttttatg ccggaaaaga gctgcttctt tgtagttgat
      361 ttgggtacga tgactttaaa gggcctttcc catgacacct ctgctttttt gcccaggaca
      421 ccgaaagttc cgtcctttcc gagagcggca tttatatttt ttttgcttaa agccattgct
      481 ttatatccct tgtaatagtg atcgaacatt tttattgtat ctttgcagtt tttgtcagag
      541 cctgaacctt tcataacaac tgctatcagg tccatatcac ctcgttttac atatgttaca
      601 agattgaatt tcgactcggt tatatatcct tttttcccgc ctacaacacc ttcatattca
      661 aaggaatgaa atcttatcat tctatggttg ttgtaaagat atcttttttc atcgcattta
      721 ttgctttttg gtataacatg cttagatgtt cccagtattt ttacaaaggt ttcgtttttg
      781 attgcatagt tcatggctat ggccagatct cttgcactgc tgtaatgctc tttatggaaa
      841 agtccgcagg tattggcaaa atgagaatca ttcatttcca gctcggcagc ctttttattc
      901 atcatctcta caaaggcctt ctcggatccg gcaacatgtt caccaagagc tattatacaa
      961 tcgtttgccg aaaccagaag agctccgtaa agggcatctt ctacagttat cttctcgccg
     1021 ggacgaaggg ctatatgagc cccacggtgg cttatgcctt tcatcactgc attgtttgaa
     1081 acagttacta tatcagtaag gtcggcattt tcaataacta caataacaga aagtatcttt
     1141 gtgcagctgg cgggataaag ccttttatct gcctcttttt caaaaagtat gttgccgctt
     1201 ttcgcatcca tgagtactgc ggatctggca tttattttgc ccatgggttt ttcgctgacc
     1261 ggtatgtctt cggtttcagc gataactgct gagaagccac taaaaaatac cgtcactgta
     1321 atggcaacgg ctgtaatagc gttaagtatt ggcattgaaa agaaagtttt tttgctgttg
     1381 ttttttttgt tcatttcaca tcctgtattt taccggtcac gcaaagaatt atacaataaa
     1441 aaccgggaag tgaaaagaga aaactgcatt tattgacaaa tataataaag cgtgtgataa
     1501 taactgaaaa atatacaaaa gctctgcaga tacagaatat gattcaaaaa ggcaattaaa
     1561 atggatatga aaaaagctat cgaaatgaga agatccaaaa gaaaatatat agatataccc
     1621 cttgatgaag aaatgatttc catgctgcga aaatccatgg aaacatataa cagagaaggc
     1681 ggtgtcgata taaggatggt gcttggagac gaaggagctt tcggcacagc tataggcggt
     1741 atcaaaaaga gttacggact tttttcggga gtaaaaaatt attttatact gataagtaaa
     1801 ttgagcagag aggagcattt tgatccgctc atgtatgaaa aactgggtta ctacggagaa
     1861 aagcttgtac ttgaggcaac ggcaatgggt cttggcacat gctgggtggg aggaacatat
     1921 gataaggaag ctgtagtctg cagccttgac aaggatgaag aaatagcatc cctcatagcc
     1981 ttcggagcca cagccgataa aattcccata agcgaacaga tcgtgggcaa ggcgttgcac
     2041 tacagaagat caaaggaagt aaaggatatg ctcaggactt ccggggatgt tgaaaactgg
     2101 atcatcgaag gaatgaggtg tgtcgtaaaa gctccctcct caatcaatat gcagccggtc
     2161 atttttacgg catcggggga tatggtgtca gcgaaggtga agggcaaaag agccatggag
     2221 ttttttgacc tgggaatcgc caaactgcac tttgaaatag gcgcgggagg agggcgatgg
     2281 gattttggca acggaggggt ttttcagccg cccccggagc gacatcagga tcgatgaaaa
     2341 ataaaaacct gccttgcggc aggttttaag tttcagaaga aaatcaacta tctgttttct
     2401 ctgtaggctc ttctttcagc cttttttttg tctctgcatt ccttgcatct ttttggctcg
     2461 ttgtcgaatc ccttttcttt gtagaattcc tgttcgcctg ctgtgaatac gaattcctgt
     2521 ccgcactctt cgcagatcaa tgttttgtct tccatggtgt tcctccaaat gaatcataga
     2581 aataacttaa tacttgttaa ctttatggga ctattctctt gcaggaacat ctttgtttaa
     2641 gactccaaaa gatgaaagga cctataaaat tctttgtaca gcataattat atgattattt
     2701 tttgtatttg tccacacttt tttttaaagt gggactattt ctgtttccat ggttatatcc
     2761 agtttggcgg cacattctat gtaataatct acctgctcat cagtaagcat gtcagaggat
     2821 atcagaccct tttgtttgat tttgtcattt acaaacattt ttgaaaacag gaagcctccc
     2881 tggccggtca ggtacatttc tgccgagagt cctgatcttt cgaaggaatc cacaaggccc
     2941 ggcgcaaata tatgagaatc gaccataaca tttttgccgt cttttttgcc gtaggcttcg
     3001 atgaccattg cacctgtatc tgaaacaagt ccttcttcta tgatatcttt tacttcttca
     3061 tggtatttgg gagccggggg tacatgtgcc aaaacgacat caaatggtat caccttacga
     3121 ccctttattt cttcttcctc ccgggaaaga aggccggctc tgtaaagggg cctcgcaaag
     3181 tctatgccca caccaccgta tttgaaataa atatttttta cacctttaaa aaatttttcg
     3241 gaattgagtc ccatgtaaac aggttcatca tgagcatgtt ccaccattgt tacgctttcg
     3301 ccgcccattt cggggaattg tctggttaaa ggtttgctga agggctgcgt ccttattatt
     3361 ctgccatttt caaaggcata ggcctcctcg ctcatgtcgg ataaggcaac taaaggtgac
     3421 caccagaacg gaaggaagcg ctttgactcc acaccttcat agaccatcat gtttatggta
     3481 tcacaggtgt ccagttctct tatggttttt cttgccatca cacatatcag acccggggcg
     3541 gaacctgtac cgatcaaagc cgtctttccc gcttttttga aacgctcacc atgatctttg
     3601 tacatgatct ttattccttc tacccattca tcggcagggt cctcgcagtg agcaacattt
     3661 tcgcaggctg caaagtcctg ataatccgcc tttacttcta tggcagtttc catgacgttt
     3721 ataccgtaat ccagcggcag ggcatttact ataagatctg ttcccgcagc cagtttcatt
     3781 atttcttctt tctttgacgc atccactttt gctgctttgc ctttttttag tatacccaca
     3841 agggagtcca ccgctttttt gtcatagtct gcacatatta tctctgatac atttggttct
     3901 tcatccagtc tttgagcaac ggtagaacct tgagcaccgg tgccaagtac aagtatcttt
     3961 ttcatttctt cctcctttat aaaaccgatt taagtttagc acagcaaaaa aaaccgggca
     4021 agaaacagga agaatatttt aaaaatgttg aaatttgaac agaaaagggg ccatatgata
     4081 taaaaggacc caaaaagcat aaaagttttg ctttttaatg gattgaaatt tgtgattctt
     4141 tttccggaaa agacttaaga acagaagact tttcataaat agcaaaatca aaaagcaaat
     4201 taaacaataa aagcgtttaa accccgatgt acaatatagt aagataaata aatagatcaa
     4261 ttcttttctg ttgcattttg ggtctgtaaa gtagatatga aaaaaagagg ccatgagaat
     4321 aatgtttctg ctaa
//
LOCUS       DAONHK010000095         4315 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_15988_length_4315_cov_3.333099, whole genome shotgun sequence.
ACCESSION   DAONHK010000095 DAONHK010000000
VERSION     DAONHK010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4315
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15988_length_4315_cov_3.333099"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1672)
                     /locus_tag="PLM92_04320"
     CDS             complement(<1..1672)
                     /locus_tag="PLM92_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006059883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HPR24393.1"
                     /translation="MKRQKIINIAVIAHVDAGKSTLVDAFLNQSGVFRENETVADCVM
                     DSDDIERERGITIYSKNCSVMHDDIKINIVDTPGHADFSSEVERIMKTVDTVILLVDS
                     AEGPMPQTRFVLKKSLEQGIVPILLINKIDKKDARIKEVVDEVYELFMDLNATDKQLD
                     FPILYGIARQGIVVYDPEDVKGIDVFDKGTKKKKNAAGESGLDIKPLFDTIIRQCSFY
                     PDHVSEPLQLQISSLGYDDYIGRLGIGRVTKGVISEASEALLCRGDSIREKRKINQVF
                     IYRGMKRAAVPNAQCGDIVVISGISDISIGDTLCDEKSPEPMEMINIEEPTLSMDFIV
                     NKSPFAGKSGKYVTSRHIKERLFRELEVNVGLTVEETDSTDVFKVSGRGELHLSVLIE
                     NMRREGYELAVSKPEVIMKKGEKGEKLEPFEELILSVPDSYTGAVISKLNIRKGIMTE
                     MISENGYSKINYILPTRGLLGYRTEFINDTRGEGTMVRSFKCFDIYKGEMPGRTNGVV
                     IAQEPGTATSYALFNIQERAVLFIEPGIKVYEGMIVGVAARAEDMVVNP"
     gene            complement(1730..3637)
                     /locus_tag="PLM92_04325"
     CDS             complement(1730..3637)
                     /locus_tag="PLM92_04325"
                     /inference="COORDINATES: protein motif:HMM:NF021489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HPR24394.1"
                     /translation="MKRSLKNILAIFSAVALIIVFSAPVVFAADTNMKTMDFDVQVNV
                     KEDKSAYITEKIKVKFLEPRRGIFRNIPYRGTTYEKIDGKKVETNYHNKIEDITVSDY
                     NYETYNEDGFTVVKIGEEDKTIKGIHAYTISYNYRMAADKSKNSDTLYLNLLPKDWPT
                     AIDNTDITVNMPKSFDEDELEVFAGGIGDNINKVKWDVKGNTIHIHSTERLLAGAGTT
                     IRLVLPEDYFEGELSYDWAITLMLITGILAILALVFFWFRYGRDPKHIQTVEFDPPEE
                     VSPAEMGYIIDGISDKKDLISLIIYFAHKGYLTIEEADEKGRDFTLRKLKDLPEDSLT
                     YELTFFNGLFDRGQGDTVDLSQLGTGFYPTFAASQKQLADEFIKKKEKRIFALSSVRA
                     RLAAYAAVAAALLVSGLLTCVFTGTFSIMIGFAVALVLVILSYTISAKEHDKKYIRKK
                     TTKTLTMILSIICLLAGMVATFFIIYWMTGELIPALILVLMEAVGLWATRYMQKRTRR
                     GAEMLGKILGFKEYIKVADKARIEMMVNENPEYFYDILPYAYVLGLTKKWAKKFENIV
                     IEPPTWYYGDYRYDTFSALIFVNSFNHYTSAMDSSIVLPSSSVEGGGGSFGGGGGFSG
                     GGMGGGGGGSW"
     gene            complement(3639..4202)
                     /locus_tag="PLM92_04330"
     CDS             complement(3639..4202)
                     /locus_tag="PLM92_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007049709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HPR24395.1"
                     /translation="MTSTIILIVILAVVAVIVIWFIAAYNGFIKLRNNVEEGFATMDV
                     YMKKRFDLIPNLVETVKGYASHEKETFRKITDARAAVQNAGSIEERAAGENILSGTLK
                     NLFAVAENYPDLKANQNFMDLQSQLKKVEEDIANSRKYYNAVVKQFNTKTEVFPSNII
                     AGMFKFERKKMFEVETEEERKNVKVGF"
     gene            complement(4214..>4315)
                     /locus_tag="PLM92_04335"
     CDS             complement(4214..>4315)
                     /locus_tag="PLM92_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HPR24396.1"
                     /translation="LSAYKGFFGGKYFSAANDFLSKFGEPVKWAIEE"
BASE COUNT         1096 a         1016 c          767 g         1436 t
ORIGIN      
        1 aggggtttac caccatgtct tccgctctgg ctgcaacacc gactatcatt ccttcataaa
       61 cctttatccc cggttcaata aaaagcactg ctctctcctg tatattgaat aaagcatatg
      121 atgtggcagt tcccggttcc tgggcaatga ccacaccgtt tgttctgccg ggcatctctc
      181 ccttataaat gtcaaaacac ttgaaactcc ttaccatcgt tccttctccc cgggtatcgt
      241 tgataaattc tgtccgatac ccgagaaggc ctcttgtggg cagaatatag ttgattttag
      301 agtatccgtt ttcgcttatc atttccgtca tgatgccttt tcgtatattt aattttgata
      361 ttactgcgcc ggtataagaa tcgggtacag ataatatcag ttcctcgaag ggttcaagtt
      421 tttctccttt ttcacctttt ttcattataa cttccggttt gctcacagcc agttcgtatc
      481 cttcccgtct catgttttcg ataagcacag aaagatgaag ctctcccctg ccgctaacct
      541 tgaaaacatc cgtcgagtcg gtttcttcca cagtcagccc cacatttact tccagttctc
      601 tgaaaagtct ttcttttatg tgacggcttg ttacatattt gcctgatttt cctgcaaaag
      661 gagacttgtt cactatgaaa tccattgaga gggtaggttc ctctatattt atcatttcca
      721 tgggttcagg tgacttttca tcacaaaggg tatcacctat ggatatatca gaaatacccg
      781 atatgaccac aatgtccccg cactgggcat ttggcacagc cgctcttttc atgcccctgt
      841 atatgaacac ctggtttatt tttctctttt ctctgatgct gtctccccgg cacagaagtg
      901 cctccgaggc ttcacttatt accccctttg tcactcttcc tattccaagt cttcctatat
      961 agtcatcata gccaagactt gatatctgca actgaagagg ctcagaaaca tgatccggat
     1021 aaaaggagca ctgcctgatg atagtatcaa aaagaggctt tatatcaagt ccactttcgc
     1081 ctgcagcatt ttttttcttt tttgtgcctt tgtcaaaaac atcaattcct tttacatctt
     1141 caggatcata aaccactatc ccctgtcttg ctataccata gagtatggga aaatccagtt
     1201 gtttatccgt agcattaaga tccataaaca gttcatatac ttcatcgacc acttctttta
     1261 tccttgcatc ttttttatct attttattga tcaaaagtat gggtacgata ccctgctcaa
     1321 gggatttttt cagtacgaat cttgtttgag gcattggtcc ttctgcggaa tccaccaaca
     1381 gtattactgt gtccactgtt ttcattatac gttccacttc agaagaaaaa tccgcgtgcc
     1441 ccggtgtgtc cacgatattt atttttatat catcgtgcat aactgaacaa ttcttggaat
     1501 atatggttat tcctctttcc cgttctatat cgtcactgtc cattacacaa tccgccactg
     1561 tttcgttttc acgaaaaaca ccactttggt tgagaaaggc atctaccaaa gtggattttc
     1621 ctgcatctac atgcgctatt accgcaatat tgattatctt ttgtctcttc atgtatttat
     1681 tgtaacatat cagacggctg tttatgaata tttctttatg aaagtttcat caccatgagc
     1741 cgccgccgcc gccgcccatg ccgcctccgg agaaaccacc gcctccgccg aagctgcctc
     1801 cgccgccttc cactgaagat gagggaagca ctatgctgct atccattgct gaagtatagt
     1861 ggttaaaaga gttgacaaaa ataagggctg aaaaggtatc ataacgataa tcaccgtaat
     1921 accatgtggg aggttcaata acaatgttct caaacttttt ggcccacttt ttggtaaggc
     1981 caagaacata agcgtaggga aggatatcat agaaatattc cgggttttcg tttaccatca
     2041 tttctatcct tgctttgtct gcaactttta tgtattcttt gaaaccaagt attttcccca
     2101 gcatttctgc gcccctcctg gtccttttct gcatatatct ggtggcccac aatcccactg
     2161 cttccatcaa tacaagaatc agtgcaggga tcagttcccc tgtcatccag tatatgatga
     2221 agaaagtggc aaccatgccg gccaaaagac atatgatact taggatcatt gtcaatgtct
     2281 tggtggtttt ctttcttata tacttcttgt catgttcttt tgccgatatc gtatatgaaa
     2341 gtatcacaag caccagggcc acggcaaatc cgatcattat gctgaaggtg ccggtaaaca
     2401 cacaggtcag aagtccggac accagcagtg cagccgccac tgccgcatat gcggcaagtc
     2461 ttgcccttac actggaaaga gcaaatatac gcttctcctt cttttttatg aattcgtctg
     2521 ccagctgctt ttgggaagca gcaaatgtgg gataaaagcc cgttcccagc tgggaaaggt
     2581 ctacagtatc tccctgtccc cggtcaaaca aaccgttgaa aaaagtaagc tcgtaagtca
     2641 ggctgtcttc aggcagatcc tttaacttcc taagtgtgaa atcacgtccc ttttcatcgg
     2701 cttcttcaat agtcagataa cctttgtgag caaaataaat tataagagaa atgagatctt
     2761 tcttatcgct tatgccgtca attatatatc ccatttctgc cggggatact tcttcgggag
     2821 gatcaaattc cacagtctgt atgtgttttg gatctcttcc gtatctgaac cagaaaaaga
     2881 caagggcaag tatggccagt atgcctgtta tcagcataag tgttatagcc cagtcatagc
     2941 tcagttctcc ttcaaaatag tcttccggca gcacaagcct gatggtggta ccggcacctg
     3001 caagaagtct ttcggtgctg tgtatgtgta ttgtgttccc tttgacgtcc catttgactt
     3061 tattaatgtt atcaccgata cctccggcaa aaacttccag ttcatcttca tcaaagcttt
     3121 ttggcatgtt tactgttata tcggtattat caatggcagt tggccagtct tttggcaaaa
     3181 gattaaggta aagagtgtca gagttttttg atttgtccgc tgccatcctg tagttataag
     3241 agatagtata ggcatggatg ccttttatag ttttatcctc ttcccctatt ttcaccacag
     3301 tgaatccgtc ctcattatat gtttcataat tataatcgga aacggttata tcttctattt
     3361 tgttatgata atttgtttcc acctttttgc cgtcaatttt ctcgtaggta gtccccctat
     3421 aaggtatatt tctgaatatg cctcttctgg gttcaagaaa tttgaccttt attttttctg
     3481 ttatatatgc ggatttgtct tctttcacat ttacctgcac atcaaaatcc atggtcttca
     3541 tattggtatc ggcagcaaat accaccggag cagaaaacac aattatgagc gccactgcag
     3601 agaagatcgc aaggatattt ttcaatgatc ttttcatatc aaaatcccac cttaacattt
     3661 tttctctctt cttctgtctc aacttcaaac atcttctttc tttcaaactt gaacatacct
     3721 gcaattatat tgctgggaaa tacttctgtt ttggtattga actgtttcac cactgcatta
     3781 tagtattttc ttgaatttgc tatatcctct tccacttttt tgagctgtga ctgaagatcc
     3841 atgaaattct gatttgcttt aagatcagga taattctcag ccaccgcaaa aagatttttc
     3901 agtgtcccgg agagaatatt ttctcccgct gctctttctt cgatgcttcc cgcattctgt
     3961 acggcggctc ttgcatcggt aattttccgg aaagtttctt tttcgtgaga tgcataccct
     4021 tttaccgttt caaccagatt gggtataaga tcgaatcttt ttttcatgta gacatccatt
     4081 gtagcgaaac cttcttctac attgttcctt aattttatga aaccgttata ggccgctata
     4141 aaccagatga ctattacggc caccaccgcc agtattacta ttagtattat tgtgcttgtc
     4201 atgtaaatac ctcctattct tctatggccc attttacggg ctcgccgaat tttgacaaaa
     4261 aatcattggc agcagaaaaa tattttccgc cgaaaaaacc tttataagcc gaaag
//
LOCUS       DAONHK010000096         4253 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_16334_length_4253_cov_3.546212, whole genome shotgun sequence.
ACCESSION   DAONHK010000096 DAONHK010000000
VERSION     DAONHK010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4253
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16334_length_4253_cov_3.546212"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3296
                     /locus_tag="PLM92_04340"
     CDS             <1..3296
                     /locus_tag="PLM92_04340"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPR24397.1"
                     /translation="LEFSSDADLDTVSVDIRDKIDLVSGSWNENVTKPTVMKLNPDMM
                     PVTVAAVNMKGKTSSRISDLLNEELLRQLEGTDGVASVSGSGMVENSIHVELDQDKID
                     KTNKKVRDAVLSQYVQASGQLEGGISYAEAQQDKTDDSKKEITEGQVELAKQTEASRI
                     LLQRNKDQLEAQLSLDPSLAPYIVPRISAIEEAISNLNIQSQIGSSRLSNAMSETVSA
                     GAMLSMTVDQLQSSLNEINTQRDSALSSADLTGILTMSNISSILGAQNFAMPAGYITE
                     NGTDMMVSLGDKFKNISEIEELVLLDTGIPGTEPVTVADVAQVRDFSDVENVYAKING
                     EDGVLLTFSKQSDYPTTETAANIQDKFKELEKEYDGLEFTTLSDQGEYIYMAIDSVLQ
                     NLFLGAVLAILILMFFLRDIRPTLITAFSIPISLTFAIALMYFTGVTLNVISLAGLAV
                     GVGMLVDNSIIVIENIYRLRALGYGKIQAAFSGVKQMSGAITASTFTTVCVFIPIVFV
                     EGITREIFVDMALTVTYSLMASLFVALTLVPVMGRGFLKTVSANTVLSRNSKYVLKYR
                     KAVRYVLAHKSRVMIIATLLLVASSAVTFSRGFEYMPPMSTPEISVDIELPKENTLQE
                     TAETCDRIAEKIRNTEGVETVGVMLSSNTAAMFGFTADLKDYSRVSMYVLLDKDKTEL
                     NPSISKKIKEAGEKYGCEMIVSGDVDMSSYSAMGGSGIVANIYSDDLDDLREFSSRVE
                     KKLRTVKGTDNISDSEEDSVPEIKITVNKNKAMEKGFTTAQIYAQVSAALATEQNATS
                     MTYEGNKRDIVVTASENSKTDLEKLKDMELVASNQAAGEAAAGEGGTEKVKLSEIADI
                     SKDKALETITHDKQKRTIQVTSEVKNGQNITKVTEKARDSVEKMDIPSGTEVEFTGEN
                     ETIMDAMKQLVQMLLLGLLMIYLVMVAQFQSLRSPFIVMFTVPLAFTGGLLGLLITGN
                     DISVVSMIGFIMLMGVIVNNAIVLIDTVNRLRKEGMDKREAIEEAGSIRLRPVLMTAL
                     TTALALTPMAFGFGEGAEMMQPVAIVCIGGLIYATFMTLLVIPAMYDVLSRKKTSVIS
                     EEELTIIDE"
     gene            3317..3403
                     /locus_tag="PLM92_04345"
     tRNA            3317..3403
                     /locus_tag="PLM92_04345"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3351..3353,aa:Leu,seq:caa)
     gene            3442..3861
                     /gene="yajC"
                     /locus_tag="PLM92_04350"
     CDS             3442..3861
                     /gene="yajC"
                     /locus_tag="PLM92_04350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HPR24398.1"
                     /translation="MNSSTLLTFGPLILIVVIMYFLLIRPQKKREKQVNQMRANLKVG
                     DEIITIGGICGKIVKTKDESIIVQVGADKLKFEMMKWSVSKVVESASRPAQKAEEEPE
                     ETAEETKRSRPRRMKKAAVAEDIKEDADTDAAEDTEK"
     gene            4012..>4253
                     /locus_tag="PLM92_04355"
     CDS             4012..>4253
                     /locus_tag="PLM92_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecDF"
                     /protein_id="HPR24399.1"
                     /translation="MNDTLKKVFAVLVIIIIAFGWYWSVAGRGSVGPLKDKIKLGLDI
                     KGGVYVVLEAQTNKKGEKLKDLMEQTQAVIEGRVNQ"
BASE COUNT         1388 a          784 c         1035 g         1046 t
ORIGIN      
        1 tgctggaatt cagcagtgat gcagatcttg atacggtatc cgttgatata agggataaaa
       61 ttgatcttgt ttcaggcagt tggaacgaga atgtgacaaa gccgacagta atgaaattga
      121 atcctgatat gatgcccgtt acagtggcgg cagtaaacat gaagggcaaa acaagttccc
      181 ggatatccga tcttcttaat gaggaactat taagacagct ggagggtaca gatggtgttg
      241 ccagtgtttc cggttccgga atggtggaaa acagtataca tgtggaactt gatcaggata
      301 aaatagataa aacaaacaaa aaagtcagag atgctgtact gtcccaatat gtacaggcat
      361 caggtcagtt ggaaggcggg atctcctatg ccgaagcaca gcaggacaaa acagatgatt
      421 caaaaaaaga gataacggaa ggccaggtcg aactggcaaa acagaccgaa gcctcccgca
      481 tactgcttca aaggaataag gatcagctgg aggctcagtt gagtttggat ccttcactgg
      541 ccccctatat agttcccagg ataagtgcaa ttgaagaagc catctcgaat ttaaatatcc
      601 agtctcagat aggatcttca agactttcaa atgccatgtc agaaaccgtt tcggcaggtg
      661 cgatgcttag catgacagtt gatcagcttc agtcttctct gaatgaaata aacacacaac
      721 gcgactccgc cttaagcagt gctgatctga cagggatcct gacaatgagc aatatctcct
      781 ccatattggg cgcacagaat tttgccatgc cggcgggata cataacagaa aacggtactg
      841 atatgatggt gtctttggga gataagttca aaaatatcag tgagatagaa gagctggtac
      901 ttttggacac gggtatcccg ggaacagagc ctgtaacagt ggcagatgtg gcccaagtca
      961 gggatttcag cgacgttgaa aatgtctatg ccaagatcaa cggagaagac ggggtgcttc
     1021 ttactttcag taagcaatcc gattacccta ccacagaaac ggcggcaaat atacaggata
     1081 aattcaaaga acttgaaaag gaatatgacg gacttgagtt caccactctt tccgaccagg
     1141 gcgaatacat atacatggcc atagattctg ttcttcagaa cctttttctc ggagccgttt
     1201 tggccatatt gatacttatg ttttttttaa gagacatacg acctactctg ataactgctt
     1261 ttagtatacc cataagtctt acctttgcca tagcacttat gtatttcacc ggggtcacgc
     1321 tcaatgtgat ctctctggca ggtcttgccg taggtgttgg tatgcttgtc gacaattcga
     1381 ttatagtaat agaaaatata tatcggctga gagctctggg gtatggcaag atacaggctg
     1441 cattttcggg cgtgaagcag atgtcgggag caataacagc atcgaccttt acaacagtat
     1501 gtgtatttat acccatcgta tttgttgaag gaataacaag ggaaatattt gtggatatgg
     1561 cccttacagt gacctattct ctgatggcaa gtctgtttgt ggctcttacc ctggttccgg
     1621 tcatgggcag aggatttctg aaaactgtat cagcaaatac ggtgctttcc aggaacagca
     1681 aatatgtttt gaaatacaga aaggctgtaa gatatgtgct ggctcacaaa tcaagagtga
     1741 tgataattgc cacactgttg cttgtagcat cttctgcggt gaccttttcc aggggctttg
     1801 aatatatgcc gcccatgtcc acgccggaga tatctgtgga tatcgaactt ccaaaagaaa
     1861 acaccctgca agagacagca gaaacatgtg acagaatagc agaaaaaatc aggaacacag
     1921 aaggtgttga aacggtgggg gtcatgcttt catccaatac tgcggcaatg tttggtttta
     1981 ctgcggatct caaggactat tccagggtaa gcatgtatgt tctcctggat aaagataaaa
     2041 cagagctgaa cccgtccata tcaaaaaaga taaaagaagc cggagaaaaa tacggatgtg
     2101 aaatgattgt ttccggtgat gtggacatga gcagttattc agctatggga ggcagcggca
     2161 tagtggccaa tatctactca gacgatcttg atgacctaag agaattctca tccagagttg
     2221 agaaaaaact gagaaccgta aaagggactg ataatatatc tgatagtgaa gaagattctg
     2281 ttcccgaaat taaaataacg gtaaataaaa ataaagccat ggaaaaagga tttacaactg
     2341 cgcagatata tgcacaggta tctgcagccc ttgcaacgga acagaacgcc acatctatga
     2401 catatgaggg aaacaaaaga gacatagtgg tgacagcatc agaaaacagt aaaacagatc
     2461 ttgagaaatt gaaagacatg gaacttgttg cttcaaacca ggcagcagga gaagcggcgg
     2521 ccggcgaagg tggtacagag aaagtaaaac tatccgaaat agccgatatt tcaaaggata
     2581 aggctcttga aactataaca catgataaac agaaacggac catacaggtc acatctgaag
     2641 tgaaaaatgg acaaaatata accaaggtaa cggaaaaggc cagggattct gtagaaaaaa
     2701 tggatatccc atcaggcaca gaagttgaat tcaccggaga aaatgaaacc ataatggatg
     2761 ccatgaagca gctggttcag atgcttcttc tggggctttt gatgatatac cttgttatgg
     2821 tcgctcagtt ccagtcatta agatctccct ttatagttat gttcacagtg cctcttgcat
     2881 ttaccggagg acttctggga cttcttatta caggaaacga cataagtgtt gtatccatga
     2941 taggatttat aatgctgatg ggtgtgatag taaacaatgc aatagttttg atcgatacag
     3001 taaatcgtct gcgcaaagag ggaatggaca aaagagaggc catagaagaa gcgggatcaa
     3061 taagattaag acctgttctc atgaccgctc ttactactgc acttgcactc acgcccatgg
     3121 ctttcggttt tggggaaggc gctgaaatga tgcagcctgt ggccatagta tgcataggtg
     3181 gactcatata tgccactttt atgacactgc ttgtaatacc cgctatgtac gatgttttga
     3241 gccgcaaaaa aacatctgtg atttcagaag aagagttgac gataatcgat gagtaatgat
     3301 ataattactc cattgtgccg gcgtgatgga attggcagac gtgcgggatt caaaatcccg
     3361 tggtgacaac accgtatggg ttcgactccc atcgccggca ccagaagaac cagttttaaa
     3421 atgtctaagg aggatatatt tatgaatagc agcaccttgt taactttcgg accacttatt
     3481 ttgatcgtgg taatcatgta ttttctgttg atcaggcctc agaagaaaag agagaaacag
     3541 gtcaaccaga tgagagccaa cttgaaagtg ggtgacgaaa taataactat aggcggtatt
     3601 tgcggaaaaa tcgtcaagac caaagacgaa tctatcattg tacaggtagg tgccgataaa
     3661 ctgaagtttg aaatgatgaa atggtcggta tcaaaagttg ttgaaagcgc atcaagacct
     3721 gcccagaaag ctgaagaaga accggaagaa acagctgaag aaacaaaaag aagcagaccg
     3781 agaagaatga aaaaagctgc agtggcagag gatattaagg aagacgcaga tactgatgca
     3841 gcggaagata cagaaaaata gcagatctga aaaaagaaag cgaaagccct tgtgtatcca
     3901 ggggcttttt atgtgtttaa atattggaaa atcaatggtt tttccttgtg gaagagatag
     3961 aaaagttgta taatataatg taatgcaaaa aataaaaagg tggatagaag aatgaacgat
     4021 actctcaaaa aagtctttgc ggtgctggtc attataatca ttgctttcgg ctggtactgg
     4081 agcgttgcag gcagaggctc tgtgggtccc ttgaaagaca agataaagct tgggcttgat
     4141 ataaaaggcg gagtttatgt ggtgcttgag gcccagacca acaagaaagg cgagaagcta
     4201 aaggatctta tggaacagac acaggcagtc atagagggac gggttaacca gat
//
LOCUS       DAONHK010000097         4238 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_16418_length_4238_cov_3.343055, whole genome shotgun sequence.
ACCESSION   DAONHK010000097 DAONHK010000000
VERSION     DAONHK010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4238)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4238)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4238
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16418_length_4238_cov_3.343055"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(43..285)
                     /locus_tag="PLM92_04360"
     CDS             complement(43..285)
                     /locus_tag="PLM92_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005807651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HPR24400.1"
                     /translation="MKATGIVRKVDELGRIVLPIELRRTLNIEIKDPIEIYVDDDYIL
                     LKKYEPACVFCGNAKDIKTIGGKNVCMNCIEELKKL"
     gene            complement(361..690)
                     /locus_tag="PLM92_04365"
     CDS             complement(361..690)
                     /locus_tag="PLM92_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPR24401.1"
                     /translation="MDMTITLKDAMLFLIFGGLFVLIIYLIVLIKNVITSVKNANAIM
                     EDTKTMTGIAKKRSEDVDNMLDGIKDSVTGFTDAMKGKESVIKSLSSIASAVTSLVNI
                     IKKSEKG"
     gene            complement(781..1518)
                     /locus_tag="PLM92_04370"
     CDS             complement(781..1518)
                     /locus_tag="PLM92_04370"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HPR24402.1"
                     /translation="MGPERKDKIGFGNLRLIQKPGEFCYGVDSVIISDFAAKCKKNIK
                     KAADLGTGTGIIPLILSYKTRIPEIVGFEIQRDAVELAKRNVSENGLDDRIKIIGCDI
                     KNIPRDLWGTMDMVVTNPPYVADGSGLQNPELTMAKARHEITADMNDFIHAAAKLLKN
                     KGDFYIVHRPSRLTDLLFFARKNGLEPKEMRLVCPDKEKTANIVMLHCTKDGGKELKI
                     LDPLLIYDMVGNYSEEIMGIYERECQK"
     gene            complement(1518..2441)
                     /locus_tag="PLM92_04375"
     CDS             complement(1518..2441)
                     /locus_tag="PLM92_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HPR24403.1"
                     /translation="MVNVVGIKFKNAGKVYYFDPGEFELSLGDSVIVETARGMEFGTV
                     TREAADVKKSEIVQPLKKIVRKADEKDRKIYEENLHKKQKALELCQEKIEKHELDMKL
                     IDVEYTFDNNKIIFYFTSDGRVDFRELVRDLAGVFRMRIELRQIGVRDEAKMLGGIGN
                     CGKGLCCHEWLSDFEPVSIKMAKLQNLSLNPTKISGICGRLMCCLKYENDVYMEMKKG
                     MPEQGEKIKIPEGMAIVMESNILQNKVKSRLIIEEEDKENDIHGKLSTELYIYSKEEI
                     QRLGRKAAVKTVEPEIYEEEIPEEIKELLKD"
     gene            complement(2444..2977)
                     /locus_tag="PLM92_04380"
     CDS             complement(2444..2977)
                     /locus_tag="PLM92_04380"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPR24404.1"
                     /translation="MLCSRLQKKPFEGDRNIAIISDADIMSARAANRLLKTLEEPPLG
                     TVIMLLSENPEVLPKTIISRCVRCRVAGYEEGISPLKEKAEETVYMLIEKTPFYQLKK
                     KIDFIGKDPDKAGEFLDCMEEIYGELIKGKGCRSELFEKEYIYDAVSAIEKAKADIRR
                     KISVDYAVKKMILEIGG"
     gene            complement(3003..3272)
                     /locus_tag="PLM92_04385"
     CDS             complement(3003..3272)
                     /locus_tag="PLM92_04385"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPR24405.1"
                     /translation="MIFSDIENNDILIKNLQTAINNNSVHHAYIFEAPGSVDKKSFAK
                     AFTKALLCQKEKGKGCGVCDVCIKTQNENHIDVTFLGHCCRGTAV"
     gene            complement(3259..3894)
                     /gene="tmk"
                     /locus_tag="PLM92_04390"
     CDS             complement(3259..3894)
                     /gene="tmk"
                     /locus_tag="PLM92_04390"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003347113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HPR24406.1"
                     /translation="MKKGLFITMEGPDGAGKSTQLEFIKSFLEKKGEKAIFTREPGGT
                     PISEKIRKIILDKNNSDMVPMTEALLYAAARSQLVSQVIKPALERGKIVVCDRFVDSS
                     IVYQGYGRGLGESVNVINDYAVAGCIPDITFLMKLAPDFGKNRIKTENKDRIESENSD
                     FHENVYRGYIELEKRYPDRIIVIDARGTIEEVSREIGMQLERILKNHNDFQ"
     gene            complement(3845..4030)
                     /locus_tag="PLM92_04395"
     CDS             complement(3845..4030)
                     /locus_tag="PLM92_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPR24407.1"
                     /translation="MILLKIAFVLLLCLPLAYLAVHFLGDAWNQLAFYIKRKKRIKER
                     NNEKRSVYYNGRPGRRR"
BASE COUNT          995 a          995 c          707 g         1541 t
ORIGIN      
        1 agacagggct atgcctgtct tttggctggt ttacgtgctt ttttagagtt ttttgagttc
       61 ctcaatacag ttcatgcaaa cgttcttgcc gccgatggtt tttatatctt ttgcgtttcc
      121 gcagaaaaca catgcgggct catatttctt cagcagaata taatcatcat ccacataaat
      181 ttcaattggg tctttgatct caatattcaa ggtccttctc aattcaatag gcaatacgat
      241 cctgcccagc tcatcgactt tacgtacgat ccccgtagct ttcatctata taatctcctt
      301 tcaaccaatt ctgaacaaaa ttgtaagtaa gatatattat tgcctatatt tgtaaatttg
      361 tcaacctttt tcactttttt tgataatatt gaccagcgac gtcactgctg aagcaataga
      421 tgaaagggat tttatcacgc tctcttttcc tttcatggca tctgtgaaac cggtcacgga
      481 atctttgatt ccatccagca tattatccac atcttcagat cttttctttg ctatacctgt
      541 catagtcttc gtgtcttcca taatggcatt cgcatttttg accgaagtta taacattctt
      601 tataagtact atcaaataga ttatcaggac aaaaagtcct ccgaatataa ggaaaagcat
      661 ggcgtctttc agcgttattg tcatatccat ctttctacct cccgtgtttt tggcgtgtat
      721 attattatcc cttattatat gggatttttc aatatgtttt tctgaacctg cggaatcagt
      781 ttatttttga cattctcttt cgtatattcc cattatctcc tcggaatagt tacccaccat
      841 atcataaatt aaaagaggat caagtatttt cagttcttta cccccgtctt ttgtgcagtg
      901 gagcatcacg atattagcgg ttttttcttt gtccggacaa acaagtctca tttctttggg
      961 ttcgagtccg ttctttctgg cgaaaaacaa aagatctgta agtctggacg gtctgtgaac
     1021 tatatagaaa tcccccttat ttttgagaag tttggctgcc gcatgaataa agtcattcat
     1081 gtccgccgtt atttcatgtc tggcttttgc cattgttaac tcgggatttt gcaacccact
     1141 gccatctgcc acgtatggag gattcgtcac caccatatcc atggtgcccc aaagatcccg
     1201 gggtatgttt tttatatcac agcctattat ttttatcctg tcatcgagtc cgttttccga
     1261 cacattcctt tttgccagct caactgcatc cctttgtatc tcaaatccga ctatctccgg
     1321 tatccgggtc ttatagctca aaataagggg tattatgccc gttcccgttc ccaggtcagc
     1381 tgcttttttt atgttttttt tgcatttggc tgcgaaatcg gagattatga ccgaatcgac
     1441 cccatagcag aattctccgg gtttttggat cagcctgagg tttccgaacc ctattttatc
     1501 ttttctctcc gggcccatca gtctttcaaa agctccttga tctcttcagg tatctcttct
     1561 tcgtatattt caggttccac ggtttttact gcggcttttc ttcccagcct ttgtatttct
     1621 tctttgctgt agatgtataa ttctgtgctc agtttcccgt ggatgtcatt ctctttgtct
     1681 tcttcctcaa taataagtct tgacttcact ttgttctgaa gtatgttcga ttccattact
     1741 atggccattc cttcggggat cttgattttt tctccctgtt ccggcattcc ttttttcatt
     1801 tccatatata catcattttc atatttgagg cagcacataa gtcttccgca aataccggat
     1861 atctttgtag ggttcaaaga aaggttttga agttttgcca tttttatgga aacgggttcg
     1921 aaatcgctga gccattcatg gcagcacaga ccttttccgc agtttcctat accgcccagc
     1981 attttggctt cgtctctgac tcctatttgc cttaattcta tcctcatgcg gaataccccg
     2041 gcaagatctc ttacaagctc tctgaaatcc actcttccgt cggaagtgaa ataaaaaatt
     2101 atcttattgt tgtcaaaagt atattcaaca tcaataagct tcatatcaag ctcatgcttt
     2161 tctatctttt cctgacaaag ttccagcgcc ttttgctttt tatgaaggtt ttcctcatat
     2221 atttttctat ccttttcgtc ggcctttctt acgatttttt ttaggggctg tactatttcg
     2281 gatttcttca catcggctgc ttctctggta accgtgccga attccatacc tctggccgtt
     2341 tcaactatca cactgtcgcc caaagacaat tcgaattctc ccggatcgaa gtaataaacc
     2401 tttcccgcat tcttgaattt tataccaact acattaacca ttattatcct ccgatttcaa
     2461 gaatcatttt ttttactgca taatcaactg atatttttct tcttatatct gctttggcct
     2521 tttcaatggc acttaccgca tcatatatat attccttttc aaaaagctcg ctcctgcagc
     2581 cttttccctt gataagctca ccgtatatct cttccatgca gtcaagaaat tctccggcct
     2641 tatccgggtc tttgcctata aagtcgattt tcttcttaag ctgataaaaa ggagtttttt
     2701 ctataagcat ataaactgtt tcttctgcct tttccttcag cggggaaatc ccttcttcat
     2761 atccggccac ccggcatctg acacaccttg aaattatggt tttgggcaac acctctgggt
     2821 tttcggataa tagcattatg actgtcccca ggggcggttc ttcaagggtc ttgaggagcc
     2881 tgtttgccgc tctggcactc ataatgtctg catcacttat tatggctatg tttcgatccc
     2941 cttcaaaggg ctttttttga agcctggaac aaagcatttc tattccttcg tcttttagac
     3001 ttttagaccg ccgtccctcg gcaacagtgc cctaaaaaag ttacatctat gtgattttca
     3061 ttttgtgttt ttatgcacac atcacagaca ccgcagcctt ttcctttttc tttttgacac
     3121 agcagggctt ttgtgaaagc ctttgcaaag gattttttat caacgctacc gggagcttca
     3181 aatatatatg catgatgtac cgagttattg tttatggcag tctgcaaatt ttttattagt
     3241 atgtcgttgt tttctatatc actgaaaatc attatggttt tttagtatcc tttcaagctg
     3301 catccctatc tctctgctga cctcctctat ggtccccctg gcatctatta ctattatcct
     3361 gtcgggatac cttttttcaa gttctatata tcctctgtat acattttcat gaaaatcgct
     3421 gttttcactt tctatcctgt ctttgttctc cgtctttatc ctgtttttcc cgaagtccgg
     3481 ggcaagcttc attaaaaagg ttatgtccgg tatacagcct gccactgcat aatcgtttat
     3541 gacgttaacg ctttctccca gacctctgcc gtatccctgg tatactatac tggagtccac
     3601 aaatctatcg caaacaacga tcttacctct ttcaagggcc ggtttgatca cctgtgaaac
     3661 cagctgggaa cgggctgcag cataaagcag tgcttctgtc atgggcacca tatcactgtt
     3721 gtttttgtcc aggattattt ttcttatctt ttcactgatc ggggtgcctc ccggttctct
     3781 cgtgaaaatc gctttctctc cctttttttc aagaaaactt tttatgaact caagctgggt
     3841 ggatttacct gcgccgtccg ggccttccat tgtaataaac agaccttttt tcattatttc
     3901 tttcctttat gcgtttcttt ctcttgatat aaaatgccag ctgattccag gcatctccca
     3961 gaaaatggac tgccaggtaa gcaagaggaa gacaaagcag taaaacaaat gctattttga
     4021 gcaatatcat tattctttgt ctcctttctt ttgaattatc agctatgcta ttataccata
     4081 tttttatatt tatgtgatat aataaaggga caccaagagc ttatatgatc aataaaggag
     4141 aactaaatgg accagcttcg cagaatgaca gaaatcttcc ccaaaaccca ctactggaac
     4201 gattcctgtg acttgaatga acttaagtat tcccttca
//
LOCUS       DAONHK010000098         4183 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_16729_length_4183_cov_4.051114, whole genome shotgun sequence.
ACCESSION   DAONHK010000098 DAONHK010000000
VERSION     DAONHK010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4183
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16729_length_4183_cov_4.051114"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="PLM92_04400"
     CDS             <1..183
                     /locus_tag="PLM92_04400"
                     /inference="COORDINATES: protein motif:HMM:NF015589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HPR24408.1"
                     /translation="KWNAYDFKILYENSRIKVYVTETECVFSLEKGKSKMITVYGKPY
                     RLKKRLVIKRSSGESE"
     gene            203..838
                     /gene="pgmB"
                     /locus_tag="PLM92_04405"
     CDS             203..838
                     /gene="pgmB"
                     /locus_tag="PLM92_04405"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013623955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0046352 - disaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HPR24409.1"
                     /translation="MVYEAVIFDLDGVLCHTDKYHYMAWKEIAGELGISFDRKINDRM
                     RGVSRMESLEILLENFKGNLSWQQKECYAQKKNRIYRCLLKNISEKDLEPEVKQTLDA
                     IKASGIKTAIGSSSRNTKFILEQLGLSGFFDAVADGTDITRSKPDPEVFIKAAEFLGV
                     EPKSCLVVEDAVAGLTAAAAAGMDCAAMGNLKDSAKADHYLDRFSDLLHIL"
     gene            935..1936
                     /gene="glk"
                     /locus_tag="PLM92_04410"
     CDS             935..1936
                     /gene="glk"
                     /locus_tag="PLM92_04410"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF014715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HPR24410.1"
                     /translation="MILAADIGGTKLLFGLFEVREGSKLAPKYIRRYQSRQFMSLYEA
                     TETFLSDVREETDVSDIQTACFSMAGPVVENRCFLVNLGWHVNADDMKERLPHIKDII
                     LYNDLEATAVGLKGLKSDDLVELTPEISSKETDSKAILAAGTGLGEALILGGKVFPSE
                     GGHCEFGPRTLEEIRLWHFLHEHYGHVSYEHILSGAGLCRLALFVMNEKGIKKPGFDL
                     LPEEISRRGLDGDCEICRSALDIYTGIMGAEAGNLALKSMALGGIYLGGGIPPEIISR
                     LRKEDFIRSFRAKGRLSSLLEKIPVTVILNNNTALYGSAIIAAEKEGHTGNLSLEEL"
     gene            complement(2031..3119)
                     /locus_tag="PLM92_04415"
     CDS             complement(2031..3119)
                     /locus_tag="PLM92_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPR24411.1"
                     /translation="MKKSSTPGLSKALKIFVVISLICILSIDLFYGFYIYADHKDDPS
                     KSLYHRFYQETENSLDGLYVGASSTYRYWMPTRAYEKNGQCVYNFSTASMPFAAYQYV
                     IEECAKTQDFEYVIIELRRLYKPVRNFKPLAARIILKPMKPSTTKLKAINAVVDFAKA
                     SSTDKDVDTNFINYFNYAYIVHSMLDDETFHKWTSIVDERGKYKGFKVSENSFIQTPS
                     PAITTDGKSISPIEPLFEDCLDKLIKYCKTLDCHVIFVTAPLNTEEENLAKINYALKK
                     AEDNGFDTINFNTQQMREALGVDYSRDFYNERHMNVWGAMKYTDYLSDYLDHKYETED
                     HRGREGYESWQKAARDLNDILSLNGVEQ"
     gene            3353..>4183
                     /locus_tag="PLM92_04420"
     CDS             3353..>4183
                     /locus_tag="PLM92_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HPR24412.1"
                     /translation="MTEDKIIKQNEAEESRKYIDGYIKRARAAQEEFEKMPQTKVDTA
                     VKVIAKVIHDNAGMLAEMAVTETGMGNVKDKTLKNENKANIIWNSLKGKPSRGIIERD
                     DKTGITKVAKPMGVVAAITPCTNPIVTPMSNAMFALKCGNAIVITPHHKAIKCSTETV
                     DLMRTELQKIDLPQDLIQILDMQSRENTGTLISSADVVVATGGAGMVKAAYSSGRPAL
                     GVGAGNVQCIIDQGYDYKQAVPKIIAGRTFDYGIICSGEQSFICPEADYEHILSEFEK
                     N"
BASE COUNT         1294 a          821 c          996 g         1072 t
ORIGIN      
        1 aaatggaatg cctatgactt taaaatatta tatgagaatt ccaggataaa ggtttatgtc
       61 acagaaaccg agtgtgtctt ttctctggaa aagggaaaaa gcaaaatgat aactgtatat
      121 gggaaacctt acagactcaa aaaaaggctg gtcataaaaa ggtcttccgg tgaaagcgaa
      181 taaaagacaa ggacaaaaaa caatggttta cgaagctgtt atttttgatc tggatggcgt
      241 gctgtgccat acagacaaat atcattatat ggcatggaag gagatcgcag gagaactggg
      301 catcagtttt gacagaaaga tcaatgaccg tatgcgcggt gtgagtcgta tggagagcct
      361 ggaaatactt cttgaaaatt tcaaagggaa tctttcctgg cagcaaaagg aatgttatgc
      421 acaaaaaaag aaccggatat accgttgcct gctcaaaaac atatccgaaa aagacctgga
      481 gcctgaagtg aaacagacgc ttgatgccat aaaggcatcg ggcataaaaa ccgccatcgg
      541 ctcctccagc agaaacacaa aattcatctt ggagcaactg gggctttccg ggtttttcga
      601 tgctgttgcc gacggcactg acataacccg atcaaaacct gacccggagg ttttcataaa
      661 ggctgcagaa tttctggggg tagaacccaa aagctgtctt gtggttgaag atgcagtagc
      721 gggacttacg gcggctgcgg cggcgggcat ggactgcgct gccatgggga atctcaaaga
      781 ttcggcaaag gcggatcatt acctggacag gttttccgat ctgctgcaca tattatgatt
      841 tttttgttcg ccgctactca aactgagtat gtgtcacaga gaagtactta cagaagaagt
      901 gtgcacagaa gaagtatatc aaagaggagg tttcatgatc cttgctgcag atataggcgg
      961 gacaaaattg cttttcggac tttttgaagt tcgggagggc agcaaacttg ccccgaaata
     1021 tatacgaagg tatcaaagcc ggcagtttat gtcactttat gaagcgacag agacgtttct
     1081 ttcggatgtg agggaagaaa cagatgtgtc tgatatccaa accgcctgtt tcagtatggc
     1141 gggtcccgtg gtggaaaaca ggtgttttct ggtcaacctc ggctggcatg tgaatgcgga
     1201 tgatatgaaa gaaagattac cacatataaa ggatattatc ctttataatg atcttgaggc
     1261 gacggccgtg ggacttaagg gtcttaaaag tgacgatttg gttgagctta caccggaaat
     1321 ttcttctaag gagacggaca gcaaagctat actggccgca ggcacgggac tgggcgaagc
     1381 ccttatcctg ggcggaaaag ttttcccttc cgagggagga cactgcgaat tcggtccgcg
     1441 taccttggaa gaaataagat tgtggcattt tctgcatgaa cattacgggc atgtaagtta
     1501 tgaacacatt ctttccggtg caggcctgtg ccggctggca ctgttcgtta tgaacgaaaa
     1561 gggaataaag aagcccggat ttgatcttct gcccgaagag atatcacgaa ggggccttga
     1621 cggagactgc gagatatgca gatccgccct tgatatttac accggcataa tgggagccga
     1681 agcggggaat ctcgctctta aatccatggc tctgggaggc atatatctgg gagggggcat
     1741 accgccggag ataatatcaa ggctccgaaa agaggacttc ataagatcct tcagagccaa
     1801 ggggagactt tcttctctgc ttgaaaagat ccccgtcaca gttatcttga acaacaacac
     1861 cgccctttac ggcagcgcca taatagctgc agaaaaagag gggcataccg gaaaccttag
     1921 tctggaagaa ctgtgactaa aaaaaataat tatgtataat aaaaaaacgg atgaggcata
     1981 tgacctgtcc gttttttaca tataatcgta tcaccatata attgattctt ttactgttcc
     2041 actccgttta ggctcaatat atcgttgaga tcacgcgctg ctttttgcca gctttcgtag
     2101 ccttcccgtc cccggtgatc ttctgtttca tatttgtgat caagatagtc cgaaaggtaa
     2161 tcagtatatt tcattgctcc ccatacgttc atatgccttt cgttataaaa atcccggctg
     2221 taatcaactc ccagggcttc acgcatttgt tgcgtgttga agttgatggt atcaaatccg
     2281 ttgtcttctg cttttttgag ggcatagttt atttttgcca ggttttcttc ctcggtattc
     2341 aaaggagccg taacgaaaat aacatgacag tcaagggttt tgcagtattt gataagctta
     2401 tccagacagt cttcaaacag aggttctata ggagatatac tttttccatc tgtggtaata
     2461 gcaggagaag gggtttgaat gaaggaattc tctgaaacct taaatccttt atatttgccc
     2521 cgctcatcca ctattgaggt ccacttatga aaggtttcat catcaagcat ggaatgaacg
     2581 atatatgcat agttgaagta attgatgaaa ttagtatcga catctttatc tgttgatgaa
     2641 gctttggcaa aatcaacaac ggcattgata gctttcagct ttgtagttga gggcttcatg
     2701 ggttttaaga taatccgtgc ggccagaggt ttgaaattcc ggaccggctt atataaccgg
     2761 cgcagttcga taatcacata ttcaaaatcc tgtgttttgg cacattcttc gataacatac
     2821 tgataagctg caaagggcat ggatgctgtg gaaaaattat atacgcactg tccgtttttc
     2881 tcatatgctc tggtgggcat ccagtatcgg tatgtggaac tggctcccac atagagcccg
     2941 tccagggagt tttcggtttc ctgataaaaa cggtgataaa gactttttga cggatcgtct
     3001 ttgtggtcgg catatatata aaagccgtaa aagagatcaa tggataagat acatataagt
     3061 gaaatgacta caaatatttt taatgctttt gacagtccgg gcgttgatga cttcttcata
     3121 ttttcaggcc tttccagatg gataaaaatc acttttcagt tatactaaaa caccatatca
     3181 tacaatttaa gttatacagc ataaaagtgt ccaaagcaac atgtttcatg aaaatatcat
     3241 gaataatcat catttcttca aacagtgagc aaaaaataaa tataagactt tcagggttga
     3301 gaaaaagtct ttttcgatgt aaaatataat tctatgataa ggaggaaaat aaatgacaga
     3361 agacaagatc ataaaacaaa atgaagctga agaaagcaga aaatatatag acggatatat
     3421 aaaaagggca agagcagctc aggaagaatt cgagaaaatg ccccagacca aagtagatac
     3481 ggcagtaaag gtcatagcaa aagttataca tgacaatgcg ggcatgcttg ccgagatggc
     3541 ggtgacggaa acgggcatgg gaaatgtcaa agacaaaacc ctaaaaaacg agaataaagc
     3601 caatatcatc tggaacagcc tcaagggcaa gccttcaagg ggcataatcg aaagagatga
     3661 taagacgggt ataaccaagg tggcaaaacc catgggagtg gtggctgcca taactccatg
     3721 cacgaacccc atcgtgacgc ccatgagcaa tgcaatgttt gcattgaaat gcggcaatgc
     3781 catagttata acgccccatc acaaagccat caaatgcagt actgaaactg ttgatcttat
     3841 gagaacggag cttcaaaaaa tcgatctgcc ccaagacctg atacagatcc ttgatatgca
     3901 gtccagagaa aacaccggga ctttgatatc ttctgcggat gtggtggtgg ccaccggtgg
     3961 agcgggaatg gtcaaagccg cctacagctc cgggagaccg gcccttggag tgggagccgg
     4021 aaacgtgcag tgcataatag accagggcta tgattacaaa caagcggtac ctaagataat
     4081 agccggaagg acctttgact acggcatcat atgttccggg gaacagtcat tcatatgccc
     4141 tgaggcagac tatgagcata tactcagtga attcgaaaaa aac
//
LOCUS       DAONHK010000099         4143 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_16969_length_4143_cov_4.732877, whole genome shotgun sequence.
ACCESSION   DAONHK010000099 DAONHK010000000
VERSION     DAONHK010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4143
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16969_length_4143_cov_4.732877"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..691)
                     /locus_tag="PLM92_04425"
     CDS             complement(<1..691)
                     /locus_tag="PLM92_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009460623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPR24413.1"
                     /translation="MGTVEFRNVSFKYPLSEKEALKDVSLKIDASEFVVVCGKSGCGK
                     TTLLRQLKKNLIPYGRLEGEVLYDGECPSGTDDRKNAAEVGFVRQDPDNQIVTDKVWH
                     ELAFGLESLGLDNGTIKRRVAETASFFDIQIWFRKNTDELSGGQKQMVNLASVMVMQP
                     RLLILDEPTAQLDPIAASEFLRTIHRINREIGTTVILSEHRLEEAFIMADRVVVMEEG
                     KILCDDVPAKVG"
     gene            complement(664..1569)
                     /locus_tag="PLM92_04430"
     CDS             complement(664..1569)
                     /locus_tag="PLM92_04430"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HPR24414.1"
                     /translation="MFKEGLGETSKFSEYHPTVNLIYFVFVIGITMFATSPVFLVVTF
                     FCAWFYSILLRGYKAIKINIVFMLPIFFVMVFVNTFFNHNGETVLFYLNKAPITKEAL
                     VFGIAAAIVLTSVVIWFMCFNVIMSADKFIYIFGRTAPILGLLLSMTFRFIKLLKVRF
                     REISDGQKCLGKEGNKKILSRIRQLLKEVSILVSWSLEASIETADSMEARGYGLKGRT
                     FFHIYKFTGRDLKMMISILISGGISAAGCIMKEMTMYYYPALDFPEESWLTAVVLTFY
                     GILLLLPAVSDIYGELKWERLNSEM"
     gene            complement(1581..2420)
                     /locus_tag="PLM92_04435"
     CDS             complement(1581..2420)
                     /locus_tag="PLM92_04435"
                     /inference="COORDINATES: protein motif:HMM:NF025833.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HPR24415.1"
                     /translation="MEKQLRKKRIRRIALIVTAAVCLAAVLLNLDFEYADYGKALGKE
                     IERAEKVLQEAEPGDQKGQYDEDTIRDFKKQIEKARAVYNDKESEYEQQKKAYEKLKK
                     EIKKFKKSSNKEDIKKDKDKEKKKEDKKQKDKDDEDSRRGTDSKEEKTLDSTEKSDGK
                     ITVYIEIRNPTYDGHRSVILSETSYRTDPGKSVYDVLYYTCRNKGIHLDAGYESTYGT
                     TYVKGIDHLYEYDAGSLSGWMYKVNGSFVNYGSSSYKVKSKDRIVWLYTTDGGEDLGK
                     PIP"
     regulatory      complement(2515..2693)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(2766..3500)
                     /locus_tag="PLM92_04440"
     CDS             complement(2766..3500)
                     /locus_tag="PLM92_04440"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HPR24416.1"
                     /translation="MEKKKIYVATIAADAEEVIEKYGFGKEEDMFIYPENLDKENLPE
                     IRMPENRFLHGPFNEVYPAAIDPAARKLAMSRLNRGYEIAGNYGIEDMVLHSGYVPLI
                     YFKEWHLKESVIFWKEFIKNKEDITIYIENVMDDEPYMMLELAEKIADRRIKLCLDIG
                     HVNCVSPIPCEEWVKVLAPFLGHLHLHSNNGNKDEHLPLGKGTFDAEKAMEAAEKYCG
                     KEVTFTIESNPAEASVLWLKERGWLK"
     gene            3611..>4143
                     /locus_tag="PLM92_04445"
     CDS             3611..>4143
                     /locus_tag="PLM92_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPR24417.1"
                     /translation="MAFGIFGKKETADLIIINADIYTQDPDFPRARAVACKNGIIIAV
                     GDKEDLLDMQTSDTFILDLQGKYVTPGLIEVNGSPVMNAFFECCFFLDPQMNEDEIKE
                     ALASRFSLWSEKISSNAENADAGFTNSGLTGSDSADSGLADLEEEASDVFFAYGYDPN
                     ILSGKKTEEAAHILDEIT"
BASE COUNT         1041 a          984 c          753 g         1365 t
ORIGIN      
        1 taccgacctt tgccggaaca tcatcgcaaa gtatctttcc ttcttccatt accaccaccc
       61 ggtctgccat aatgaatgcc tcttcaagcc ggtgctcgga aagtatgaca gtggtcccta
      121 tttctctgtt tatcctgtgt atggtcctca aaaattccga agccgctatt ggatccagct
      181 gcgctgtagg ttcatcaaga ataagcagtc tgggctgcat caccatcacc gatgccagat
      241 tcaccatctg cttttggcca ccggaaagtt cgtcagtatt ttttcgaaac cagatctgaa
      301 tgtcaaaaaa acttgcggtt tctgcaactc ttctttttat ggtaccgtta tccagaccaa
      361 ggctttcaag accgaaagcc agttcatgcc agactttgtc cgtcactatc tggttgtccg
      421 gatcctgtct tacaaatcct acctcggcgg cgttttttct gtcatccgta ccggaaggac
      481 attcgccgtc ataaagcact tctccttcca gccttccgta aggtataaga ttctttttca
      541 gctgacggag caatgtcgtt tttccgcagc cggacttacc gcaaaccact acaaattccg
      601 acgcatctat tttgagagat acatccttta gggcttcctt ttccgaaaga ggatatttaa
      661 aacttacatt tctgaattca accgttccca ttttagctct ccgtatatat ctgaaactgc
      721 cggcaataac aacagtatcc catagaaagt cagcacgact gcagtaagcc agctttcttc
      781 aggaaaatcc agtgccgggt aatagtacat agtcatttct ttcatgatgc agcctgcggc
      841 agatatgcct cctgatatga gtatacttat catcattttc agatctcttc ccgtaaactt
      901 ataaatatga aaaaaggtac gcccctttag tccgtatcct ctggcttcca tggaatctgc
      961 cgtttctatg gaagcttcaa gggaccatga aacaaggatg gacacttcct ttaataattg
     1021 tcttattctg cttagtattt ttttgttccc ttcttttcca agacactttt gtccgtccga
     1081 tatttcccga aagcgtacct tcagaagctt tatgaatctg aaggtcatgg acaggagaag
     1141 tccaagaatc ggtgcagtcc tgccgaaaat ataaatgaac ttgtctgcgg acatgatcac
     1201 gttgaagcac atgaaccaga taactacaga tgtcaaaacg atggctgccg ctatgccgaa
     1261 tacaagagct tcctttgtta tcggtgcctt gttgagataa aagagtaccg tttctccgtt
     1321 atggttgaaa aaggtgttta caaaaaccat aacaaaaaat atgggcaaca taaaaacaat
     1381 gtttattttt atggctttat aacctcttaa aagaatggaa taaaaccatg cgcaaaaaaa
     1441 tgtcacaaca agaaaaacag ggcttgtggc gaacatggtg atccctataa caaaaacaaa
     1501 atatatcaga tttactgtgg gatgatactc cgaaaatttc gaggtctctc ccagcccttc
     1561 tttgaacaat ctgtgcctcc ttaaggtatg ggtttaccca gatcttctcc gccgtctgtt
     1621 gtataaagcc atacgatccg gtctttactt ttgaccttgt atgatgaact tccgtagttt
     1681 acaaaagagc cgttgacctt atacatccat ccgctcagac tgcctgcatc gtattcgtaa
     1741 agatggtcga tgcccttcac ataagtcgtt ccgtatgttg attcatatcc cgcatcaagg
     1801 tgtatccctt tatttctgca ggtatagtaa agcacatcat aaacactttt gccgggatcg
     1861 gtcctgtatg aggtttcaga aagtatcaca cttctgtgtc cgtcgtatgt tgggtttctt
     1921 atttcaatgt atactgttat tttgccgtcg cttttttctg tactgtctaa ggttttttct
     1981 tctttgctgt ctgtcccccg gcggctgtct tcgtcatctt tatctttttg ttttttatcc
     2041 tctttcttct tttctttgtc tttgtctttt tttatatctt ctttgttgct gctttttttg
     2101 aatttcttta tttctttttt cagtttttca taggctttct tctgctgttc atattcgctt
     2161 tctttgtcat tgtaaacagc ccttgctttt tctatctgtt ttttgaaatc tcttattgta
     2221 tcctcatcat actgcccttt ttgatccccc ggttcagcct cctgcaggac tttttctgcc
     2281 cgttctattt cttttcccag ggctttaccg taatccgcat actcaaaatc caggttgagt
     2341 aaaacagctg cgagacaaac tgccgccgtc actataagtg ctatccttct tattcttttt
     2401 ttccggagct gtttttccat caaatttctc cacaagactt ccaagtaaaa aactgcggca
     2461 actgccgcag tccgtttttc tgctctttca aaaactgtat accgatcgcc cgccggcaca
     2521 gcccgggatc tatgcaggtc ttctggctca ggtgtcatcg tcttgtccgt cttcccgatc
     2581 tctcagtgac ataatagaca aaactctccc cttacagcgg cgggaccgcg caggattcaa
     2641 acctgcttcc ctcttagccg gatatttccg gaacacagat catatttatt tacatggtaa
     2701 ttctaatgcc gtcaaaccaa aaagtaaagc cttatttctg ccttatgaaa tgcccccgga
     2761 tacttttatt ttaaccatcc tctttccttg agccacaaca cgcttgcttc agccggattg
     2821 ctttctatgg taaaagtcac ttcttttccg caatattttt ctgctgcttc catggctttt
     2881 tctgcatcaa acgttccttt tccaaggggc aaatgctcat ctttatttcc gttgttactg
     2941 tgcaggtgta aatgtcccaa aaatggtgca agtactttta cccattcttc gcagggtata
     3001 gggcttacgc agttcacatg cccgatatca agacaaagtt ttattcttct atctgcgatc
     3061 ttctctgcaa gttccagcat catatatggt tcatcgtcca tgacattctc aatatagatg
     3121 gttatatctt ctttgttttt tatgaactct ttccagaaaa tcaccgactc ttttagatgc
     3181 cattccttaa aatatatcag aggtacatac cccgaatgca gtaccatatc ctctatgccg
     3241 taattgccgg ctatttcata tcccctgttt aatcttgaca tggccagctt cctggcagcg
     3301 ggatctattg cagcagggta tacttcgttg aaaggtccat gcaggaatct gttttccggc
     3361 attctgattt caggaagatt ttctttatca agattttcag gatatataaa catatcctct
     3421 tctttgccaa agccgtattt ttctatgact tcctcagcat cggcggctat agtggcaaca
     3481 tatatttttt tcttttccat ggaatcacct tatcacatct gataataaca ggcaaacttt
     3541 tatgttataa tatgtttcaa agtataatac atatttcgca cactgcatga cttaagtccc
     3601 ggaggtgttt atggcttttg gtattttcgg aaaaaaagaa acagctgacc ttataatcat
     3661 caatgctgac atatatactc aggatcccga ttttccccgt gcccgggcgg ttgcctgcaa
     3721 aaacggtata ataatcgccg taggagacaa agaggatctt cttgatatgc agacatctga
     3781 tacatttatt ttggatcttc agggaaagta tgtgactccc ggacttatag aagtaaacgg
     3841 ttctcccgtc atgaatgctt tttttgaatg ctgttttttt ctggaccctc aaatgaatga
     3901 agatgaaata aaagaggctc tggcctcccg gttttctctg tggtccgaaa aaatctcctc
     3961 caatgcagaa aacgcagatg ccggtttcac aaactccggt ttaacaggtt cagattcagc
     4021 ggactcaggc ttagcggact tagaagaaga ggcttctgat gttttttttg cctacggtta
     4081 tgacccaaat atcctgtcgg gcaaaaaaac cgaagaggct gcccatatac tggatgaaat
     4141 aac
//
LOCUS       DAONHK010000100         4115 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_17122_length_4115_cov_4.057389, whole genome shotgun sequence.
ACCESSION   DAONHK010000100 DAONHK010000000
VERSION     DAONHK010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 4115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4115
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17122_length_4115_cov_4.057389"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1319
                     /locus_tag="PLM92_04450"
     CDS             <1..1319
                     /locus_tag="PLM92_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009643933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HPR24418.1"
                     /translation="GTDIILGGNPEFEAKKEMIKEGYDPEIIAYASSLIPTEDDEILK
                     ARAEFNIVHEKYKKERADEQQRVRDLGGLCIIGTERHESRRIDNQLRGRSGRQGDPGI
                     TQFCISMEDELMRLFGGERMQNVVSRLGVEEDEAIEAGMITRSIESAQKKVEGRNFSI
                     RKYVLQYDNVMNKQREIIYAERRKVLFGEDLKDYIMNMAEELIEGCIKPITVASKYPE
                     EWDLETMNANLKKICNRFKGVAYTEEGSRDLDEEILIEDVLEDFQKLYEEKEKEIGEE
                     RMRELERMILIRVVDNKWMDHIDAMDQLKEGIGLRAMGQQDPAGAYAQEGFDMFEEMI
                     EAIKEETVKFCYNVTLQTSSERRRIIGGGEDLKEEYEDDAAYLKDAVEGTDQSYEDTP
                     GQNHKVPERQKNKQQPVKRDMPKVGRNDPCPCGSGKKYKHCCGKDN"
     gene            1322..2420
                     /gene="prfB"
                     /locus_tag="PLM92_04455"
     CDS             join(1322..1396,1398..2420)
                     /gene="prfB"
                     /locus_tag="PLM92_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_156908167.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HPR24419.1"
                     /translation="MVQLEQIKNDIPASRGRLKEAGESLDPASLEKKLAGLEELTKEK
                     GFWNDNDNAQRVMKEKKSIESSLNEYREISRDLEDIEVLISLAEEAGDETMVPEIQEA
                     YREFTKKAETLRIKTLLKGEYDGNNAIIHIHAGSGGTDAQDWAAILLRMYSRWAESKN
                     CRIKTLDIQEDTEGGIKGATLLIQGSNTYGYLKNEKGVHRLVRISPFDSSGRRHTSFA
                     SVDVTPEMDDDIVVEIDEDDLRIDTFRSSGAGGQHVNKTDSAIRITHIPTNIVVSCQN
                     ERSQHQNKDMAMKILKSRLLELKEQEHKESLKELKGDYSQITWGMQIRSYVFHPYTMV
                     KDHRTGAEVGNVNKVLDGDLDMFINEMLKQG"
     gene            2616..3761
                     /locus_tag="PLM92_04460"
     CDS             2616..3761
                     /locus_tag="PLM92_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HPR24420.1"
                     /translation="MILETLVRFSEDMGLFSLDYKSAIMILVSILFLYLAIRKGFEPL
                     LLVPIAFGMLLSNLPLTGIFEAHPEAGTELADMGLLTIFYLLKPILPSLIFLGVGAMT
                     DFGPLIANPKSLILGAAAQFGIFFAFFAAIALGFDAQGAASIGIIGGADGPTAIYTTQ
                     KLAPQLLGAVAVAAYSYMALVPVIQPPIMKLLTTKKERVIKMEQLRVVSQREKILFPV
                     AVTIIVVLLLPAAAPLIGMLMLGNLFKECGRTARLSDAAANAMNNIVVIMLGLAVGAS
                     AQGPTFLVMDTIKIVILGVLAFAFGTAGGVLIAKLMNALTGGKLNPLIGSAGVSAVPM
                     AARVSQNVGKEYNRTNFLLMHAMGPNVAGVIGSAVAASVMLSMFGGY"
     gene            3890..>4115
                     /locus_tag="PLM92_04465"
     CDS             3890..>4115
                     /locus_tag="PLM92_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HPR24421.1"
                     /translation="MTEKKGKSKFDTVIFDFDGTIMDTNNIIIDSWQHTFRTIEGRER
                     PVEEIIGTFGEALGETMARLLPGHETQVALD"
BASE COUNT         1386 a          740 c         1033 g          956 t
ORIGIN      
        1 gaggaaccga cataatactt gggggcaatc cggagtttga agcaaaaaaa gaaatgataa
       61 aagaaggata tgaccccgag ataattgcat atgcttcaag tcttatacct acagaagatg
      121 atgaaatatt aaaagcacgt gcggagttca atatagttca cgaaaaatac aagaaggagc
      181 gggctgatga acagcagcgg gtcagagatc tgggcgggct ttgcataata ggcaccgaaa
      241 gacacgaatc aagaagaata gataaccagt taaggggtcg ttccggaaga cagggagacc
      301 cgggaataac ccagttctgt atttccatgg aagacgaact catgaggctt ttcggcggag
      361 aaagaatgca gaatgtagta agcagactgg gtgttgagga agatgaagcc atagaagcag
      421 gaatgataac ccgttcaata gaaagtgcac aaaaaaaggt tgaaggcagg aatttttcca
      481 tcagaaaata cgttcttcag tatgacaatg taatgaacaa acaaagagag ataatctatg
      541 cagaaaggcg caaggttctt tttggagaag atctgaaaga ttacataatg aacatggctg
      601 aagaactgat agaaggatgc ataaagccaa taacggtggc atcgaagtat ccggaggaat
      661 gggatctgga aacgatgaat gcaaacctaa aaaagatctg caacagattc aagggagtgg
      721 cttatactga agagggatca agggatctgg atgaagagat actgatagaa gatgttctcg
      781 aagactttca aaagctctat gaagaaaaag aaaaagaaat aggcgaagag cggatgcgtg
      841 agctggagag gatgatcctg ataagagttg tggacaataa atggatggat cacatagatg
      901 ccatggatca gctcaaagaa ggaatcggcc ttcgggcaat gggacagcag gatcctgccg
      961 gggcttatgc ccaggaagga ttcgatatgt ttgaagaaat gatagaggct atcaaagaag
     1021 aaactgtgaa gttctgctat aatgtaacat tacagacaag ctccgaaaga agacgtataa
     1081 tcggaggagg agaagatctc aaggaagaat atgaagatga tgcagcatac ctgaaggatg
     1141 cagtagaagg cacggaccag agctatgaag acacacctgg tcagaaccat aaggtgcctg
     1201 aaaggcaaaa aaacaaacag cagccggtaa aaagagatat gcccaaagta ggcagaaacg
     1261 atccatgccc ctgtggcagc ggaaagaaat ataaacactg ctgcggaaag gacaattgat
     1321 tatggtacaa ctggaacaga taaaaaatga tatacctgca tcaagaggca ggctaaaaga
     1381 ggcaggtgaa tctctttgac ccggcttccc ttgaaaaaaa acttgccggt cttgaagaac
     1441 ttacaaaaga gaaggggttc tggaacgaca atgataatgc ccaaagagta atgaaggaga
     1501 aaaaatctat tgaaagcagt ttgaatgagt acagggaaat aagcagagat cttgaggata
     1561 tagaagtact tatatctctt gcggaagaag caggagacga gaccatggtg cctgaaatac
     1621 aggaagcata cagagaattc acaaaaaagg ctgagaccct caggatcaaa acgttgctga
     1681 aaggggaata tgacggaaac aatgccatta tccatatcca tgccggttcc ggaggaacag
     1741 atgcacagga ctgggcagcc atccttttaa gaatgtacag ccgctgggcc gaaagcaaaa
     1801 actgccggat aaagaccctt gatattcagg aggacacaga gggcggcatc aaaggagcca
     1861 cacttcttat acaaggcagc aatacctatg gatatcttaa aaatgaaaag ggtgttcaca
     1921 ggcttgtaag gatatcccca tttgactcaa gcggcagaag gcatacatcc tttgcatcag
     1981 tggatgttac cccggaaatg gatgatgata tcgttgtgga aatagatgaa gatgatttaa
     2041 gaatagatac tttcagaagc agtggagcgg gaggacagca tgtaaacaaa accgattccg
     2101 ccataaggat cacacatata cccacaaaca tagtagtatc atgtcaaaat gagcgttctc
     2161 agcaccagaa caaagacatg gcaatgaaaa tactaaaatc aagactgttg gaactgaagg
     2221 agcaggaaca taaggaaagt ctaaaggaac taaaaggaga ttatagtcag ataacctggg
     2281 gaatgcagat acgttcgtat gtgtttcatc cttatacaat ggtaaaagat catagaacag
     2341 gcgctgaagt ggggaatgtg aacaaggtcc ttgacgggga tctggatatg tttataaatg
     2401 agatgctcaa gcagggataa accataaaaa aaaggtaaaa aaatggcttg aaaccattcg
     2461 gaccaaagga tttcttgtat acagtattta tgctcaaaaa cgatgttcaa aagcaaaatt
     2521 atcattgatt aaacaaaata agtgtgttac cattttaacg gtgtgggttt tcgcactgtt
     2581 ttattcatgt catttttgaa aaggagacag acaaaatgat tttagagact ctggtcagat
     2641 tctcggaaga tatgggatta ttcagcctgg attacaaatc agcgataatg atactggtat
     2701 ccattctttt cctttatctg gcgataagaa aaggttttga acctctttta ctggtcccta
     2761 ttgctttcgg catgcttctt tcgaaccttc ctcttacagg aatattcgaa gcacaccctg
     2821 aggccggaac agagctggcg gacatgggac tattaactat attttatctg ttaaaaccga
     2881 tattgccctc tttgatattt ctgggggttg gtgccatgac ggatttcgga cctcttatag
     2941 caaacccaaa atccctcata cttggtgccg cggcacagtt tggtattttc tttgcattct
     3001 ttgcggcgat cgctctcggc ttcgatgccc agggagccgc ttcaataggg ataataggag
     3061 gtgccgacgg gcctacggct atatatacaa cgcagaaact tgcaccacag ttgctgggag
     3121 cagttgctgt agcggcatac tcttatatgg ctctggtgcc tgttatacag cctccaatca
     3181 tgaagcttct gactacaaaa aaagaaagag tcataaagat ggaacagctt cgtgtagtaa
     3241 gccagagaga aaagatactg tttcctgttg cagtaacaat aatcgtggta cttctgcttc
     3301 cggcggctgc acctcttatt gggatgctta tgctaggcaa cctttttaag gaatgcggca
     3361 ggacagcaag actttccgat gcggcagcaa atgccatgaa taacatcgta gttatcatgc
     3421 tgggtctggc cgtgggagct tccgcacagg ggcccacctt cctggtaatg gatacaataa
     3481 agatagttat acttggagtt cttgcatttg cattcggaac tgccgggggc gtattaatag
     3541 caaaactgat gaatgccctt accggaggca agctgaatcc gcttatcggt tcagcaggtg
     3601 tttctgcagt tcctatggca gcccgtgtat ctcagaatgt aggaaaagag tacaacagga
     3661 ccaatttcct gcttatgcat gccatgggac ctaatgtggc aggtgtaata gggtctgcgg
     3721 tggccgcctc tgtaatgcta tcaatgtttg gaggttactg ataatataag cagatataaa
     3781 gcgtatttta ctccggccct gcggccggag ttttaatatc ttcgttgttg aaaccataat
     3841 gctgtgatac aatttctgta tgggagaatc agtgaggaag aaggataaaa tgacagaaaa
     3901 aaagggaaaa agcaaatttg atacggttat ttttgatttt gacggaacca taatggatac
     3961 caacaatatc ataatagatt catggcagca tacatttcgt acgatagagg gaagagaaag
     4021 acccgttgaa gaaataatag gaacctttgg agaagccctt ggtgaaacca tggccaggct
     4081 tctgcccggg catgagactc aggtggcttt ggata
//
LOCUS       DAONHK010000101         3988 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_17854_length_3988_cov_3.877702, whole genome shotgun sequence.
ACCESSION   DAONHK010000101 DAONHK010000000
VERSION     DAONHK010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3988
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17854_length_3988_cov_3.877702"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(55..888)
                     /gene="rplB"
                     /locus_tag="PLM92_04470"
     CDS             complement(55..888)
                     /gene="rplB"
                     /locus_tag="PLM92_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005999399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HPR24422.1"
                     /translation="MGIKKYKPTTPGLRGMTVADFNEITTDKPEKSLTVTLKKHSGRN
                     SRGKITVRHRGGGTRAKYRIIDFKRNKDDIPGKVATIEYDPNRSAYIALINYADGEKR
                     YILAPEGLKVGDVLMSGQKADIKSGNALPLSNIPVGTMIHNIEMKPGKGGQLVRSAGN
                     GAQLMAKEDKYVQVRLPSGEVRKVRMECRATIGEVGNEDHANINIGKAGRKRHMGIRP
                     TVRGSVMNPNDHPHGGGEGKAGIGRVSPVTPWGKPTLGYKTRKKNKDSSRYIVKRRNE
                     K"
     gene            complement(910..1200)
                     /gene="rplW"
                     /locus_tag="PLM92_04475"
     CDS             complement(910..1200)
                     /gene="rplW"
                     /locus_tag="PLM92_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002836110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HPR24423.1"
                     /translation="MKTPYDVIIKPVISERSMEDVQNRKYTFKVATDANKTQVKLAVE
                     EIFDVEVEKVNIMNVSGKLKRMGRNVGRTSSSKKAIVTLTEKSKEIEFFQGL"
     gene            complement(1200..1823)
                     /gene="rplD"
                     /locus_tag="PLM92_04480"
     CDS             complement(1200..1823)
                     /gene="rplD"
                     /locus_tag="PLM92_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HPR24424.1"
                     /translation="MAKVKVLKMTGADAGEIELKDEIFGIEPNEFAVHAVVKNYLANR
                     RQGTQSAKTRGDVRGGGRKPFRQKGTGRHRQGSTTDPTQVGGGVAFAPKPRSYRYTLP
                     KKLRRLAMISALSSKVKEKDIIVLDELKFKEPKTKEMISVLENIKAGKKALIVMADKD
                     DNIVKSASNIPGTRTTLVSAMNVYEIINHDSFIVTKDAVNKIEEVYS"
     gene            complement(1849..2484)
                     /gene="rplC"
                     /locus_tag="PLM92_04485"
     CDS             complement(1849..2484)
                     /gene="rplC"
                     /locus_tag="PLM92_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005999393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HPR24425.1"
                     /translation="MKILLGKKIGMTQIFNEEGEVIPVTVIEAGPQTVTQIKTVETDG
                     YDSVQVGFSDAKPQRINKPMTGHFKKAGVDVKKHLMEFIPDDGDSYEIGQLMTVNEFE
                     EGKLLDITGTSKGKGTQGNIKRHGHHRGPMTHGSKHKRMAGALAAGTYPSRVFKGNAG
                     PGRMGRETITVQNVKLVKVIEDRNLLLVKGAVPGGKGSLVRIQYAVKGQEQ"
     gene            complement(2554..2865)
                     /gene="rpsJ"
                     /locus_tag="PLM92_04490"
     CDS             complement(2554..2865)
                     /gene="rpsJ"
                     /locus_tag="PLM92_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008901013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HPR24426.1"
                     /translation="MGELQKIRIRLKAYDHALLDQSAAKIVDTAKKTGAEVSGPIPLP
                     TEKEVVTILRAVHKYKDSREQFEQRTHKRLIDITNATAKTTDALTKLELPAGVEIEIK
                     L"
     gene            complement(3325..3966)
                     /locus_tag="PLM92_04495"
     CDS             complement(3325..3966)
                     /locus_tag="PLM92_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015327031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HPR24427.1"
                     /translation="MSDAVKAANRLKCEEIIKNLQKRGMNGYYCENSREACEKALSMI
                     PNGTRVSWGGSVTIENIDIKDRLELVNAEIIDPMAAKDPKEAYDTRVMALMSDVFLTG
                     TNAITLDGKLVNIDGRGNRTAALCFGPKKVIIIAGTNKIVRDEEAAMARIKTEACPAN
                     CIRLKKDTPCAKTGICGNCLISGQTICSMTLVTRFCNSPERIHVIMVNENLGY"
BASE COUNT          739 a         1011 c          753 g         1485 t
ORIGIN      
        1 cgtttacgaa agggcctttc ttaagtgatc tacccatttg ttttgctcct ttccttattt
       61 ttcgtttctt ctcttaacga tatatctgct ggaatctttg ttcttctttc ttgtcttata
      121 tcccagggtc ggtttacccc atggtgttac cgggcttact cttccgatcc ccgcttttcc
      181 ttctccaccg ccgtgaggat gatcatttgg gttcattact gaccctctca ctgtgggtct
      241 tatgcccatg tgtcttttcc tgccggcctt tccgatgttt atatttgcgt gatcttcatt
      301 tcccacttca ccgatggttg ctctgcattc catcctgacc ttgcgaacct ctcccgacgg
      361 cagtcttacc tgaacatact tgtcttcctt ggccatcagc tgtgcgccgt tgcctgcgga
      421 tcttaccagc tgtccgcctt ttccgggttt catctctata ttatgtatca ttgtgcccac
      481 aggtatattg gaaaggggca gtgcgtttcc gctctttata tccgcctttt ggcctgacat
      541 cagcacatct ccgactttaa ggccttcggg agccagtatg tatcttttct ctccgtcagc
      601 atagtttatc agtgcaatat atgcgctcct gtttgggtca tattctattg tggccacttt
      661 accgggtatg tcatctttgt ttctcttgaa gtcgattatc ctgtacttgg ctcttgtgcc
      721 gccgcctctg tgcctgactg ttatcttgcc tctggaattt cttcctgaat gcttcttcag
      781 tgttactgtc agggattttt ccggcttgtc tgtggttatt tcattgaaat cggcaaccgt
      841 cattcctctc aaaccaggag tagtcggctt atattttttt attcccattg tcttctgcct
      901 cccgtctctt tacagtccct ggaagaattc gatttcttta ctcttctccg taagcgttac
      961 tatcgctttc tttgaagaag aagttctgcc gacatttctt cccattctct ttaacttacc
     1021 gcttacattc attatgttga ccttttcaac ttccacatcg aatatctctt cgactgcaag
     1081 ttttacctgt gtcttgtttg catcggtcgc caccttgaat gtgtattttc tgttttgaac
     1141 atcctccata cttctttcgg atattacagg ttttattatt acgtcataag gggttttcat
     1201 tatgaataca cctcctcgat cttgtttacg gcatcttttg ttactatgaa gctgtcatga
     1261 ttgattattt catatacgtt catggccgat accagagtcg ttcttgtccc gggtatattg
     1321 gatgctgatt ttacgatatt atcatcttta tctgccatca ctataagggc cttcttcccg
     1381 gcctttatat tttcaagaac actgatcatt tcctttgttt tcggctcttt gaacttcagt
     1441 tcatcaagca cgatgatatc cttttctttt acctttgagg aaagggcgga tatcattgcc
     1501 agtctcctga gtttctttgg cagtgtatat ctgtagcttc tgggtttggg tgcaaatgca
     1561 actcctcctc ccacctgggt gggatccgta gtgcttccct gtctgtggcg tccggtccct
     1621 ttttgtctga agggttttct tccgcctcct cttacatcgc ctcttgtctt tgcagactgc
     1681 gttccctgtc tacggtttgc caggtagttc ttgactactg cgtggacggc aaactcattt
     1741 ggctcgatcc cgaaaatctc gtctttgagc tctatttcac cggcgtccgc tccggtcatt
     1801 ttaagcactt ttacttttgc cattttctac gtcctccttc cgtaatcctt attgctcctg
     1861 gcctttaact gcgtactgta tgcgcaccag gcttcctttg ccgccgggta ccgcaccttt
     1921 tacaagcagc agatttctgt cttcgataac ttttaccagt tttacattct gaacagttat
     1981 agtctcgcgg cccattcttc cggggcctgc atttcccttg aacactctgg aaggataggt
     2041 ccctgcagcc agggcaccgg ccattctctt gtgtttggag ccgtgagtca ttgggcctct
     2101 gtggtgtccg tgtcttttaa tgtttccctg agtgcctttg ccctttgacg ttcccgttat
     2161 gtccagcagt tttccttctt caaattcatt gaccgtcatt aactgcccta tttcgtagct
     2221 gtcaccatca tcaggtatga attccatcag atgtttcttc acatccacgc ctgctttttt
     2281 gaaatgacct gtcatcggct tatttatcct ctgcggtttc gcatcggaga agcccacctg
     2341 aacggaatca tatccgtctg tttcgaccgt ttttatttgt gtgacggtct ggggccctgc
     2401 ttcgatcaca gtaacaggta taacttcgcc ttcttcattg aaaatctgtg tcataccgat
     2461 tttttttccc agtaatattt tcatatttaa cctcctgaat acttacagcg gattgcgttt
     2521 ttttgcaatc atactaaggg agttcccttc gccttataac ttgatttcga tttccactcc
     2581 tgcgggcaac tccagttttg tgagcgcatc cgtagtcttt gcagtggcat ttgtgatatc
     2641 tatcagtctc ttgtgtgtcc tctgttcaaa ttgttctctg ctgtccttgt acttatgaac
     2701 tgcccttagg atagttacga cttctttttc tgtgggaaga ggtatcggtc ccgatacttc
     2761 tgctcctgtc ttttttgccg tgtcaactat ctttgcagcc gactgatcaa gcagtgcgtg
     2821 atcatatgct ttcaatctga tcctgatttt ctgtagttct cccattttgt cctccaaatc
     2881 ttttttgtac agttttcgct attttcgtac aaggggttct ttgttggttt ttcaaggttt
     2941 caaggttaag cctgcgcgtt acacctcgcc gtgcgtttgc tgttttatgc cacgaaacaa
     3001 aaccggcaaa ccccttcgcc cccgtcttgc gggaacaatt ctcggcggaa atatcctgat
     3061 agctcagcaa cttcccgctt cttcgccatt tcgcaagctt ttgtagtata taacatatgt
     3121 attttcattg caagagtttt tttaaaaaaa cttgattgtt tttttgtggt ttttatccat
     3181 gcaaatcacc tcttttcaat gtctttatct gtcaacgcat tcacactgtt ccttgcgttt
     3241 gttatacgcg cccttctaat aaaaaaatgc atacccgact ttactgttac cgggtatgca
     3301 ttttgccgat atgatttttt attctcaata gccaagattc tcattcacca ttattacatg
     3361 tatcctttca ggactgttgc aaaatcttgt taccagtgtc atggaacata tggtctgacc
     3421 gcttataaga cagttcccgc atatacctgt ttttgcacag ggtgtgtctt ttttgagcct
     3481 tatacaattg gcgggacagg cttccgtttt gatcctggcc atagccgctt cttcatccct
     3541 tactattttg tttgttccag caattattat tacttttttt ggtccgaaac aaagagccgc
     3601 cgtacgatta cctcttccgt ctatgttgac aagctttccg tcaagggtga tcgcatttgt
     3661 gccggtaaga aaaacgtctg acataagagc catgaccctg gtgtcgtaag cctcttttgg
     3721 atctttggct gccattggat ctattatttc tgcattaaca agttccagtc tgtcttttat
     3781 gtcgatattt tcgatcgtaa cagaacctcc ccatgatacc cgggttccgt ttgggatcat
     3841 ggaaagggct ttttcgcagg cttccctgga attctcgcaa taatacccgt tcatacctct
     3901 cttttgaagg ttcttgatta tttcttcaca cttcagcctg tttgctgctt ttactgcatc
     3961 tgacattttt ctcctcctgt ctttttac
//
LOCUS       DAONHK010000102         3866 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_18724_length_3866_cov_5.384676, whole genome shotgun sequence.
ACCESSION   DAONHK010000102 DAONHK010000000
VERSION     DAONHK010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3866
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18724_length_3866_cov_5.384676"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            209..1783
                     /gene="rho"
                     /locus_tag="PLM92_04500"
     CDS             209..1783
                     /gene="rho"
                     /locus_tag="PLM92_04500"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HPR24428.1"
                     /translation="MDEKILKSKKVAELREVAKAFGVHESSTLKKEELIAALLSMADE
                     QKSGKTAAKKEMTETKEAEAEKSGAGSVKVQKKAPDKKGIRRKASEKANGFPVSAEEM
                     SERKNAKTAVQTESEEAGTIKPDENERIRENGKAEENSKIEANGKAEENGRTEAEGIL
                     DIAEGGFGFLRFSNFLTSDKDVYVSPSQIRRFNLKTGDKIKGITRAPKPGEKFGALLY
                     VNTVNGDDPGEARRRPDFDDLTPIFPDERLSLEDRSRDLAMRVIDLVAPIGKGQRGLI
                     VAAPKAGKTVLLKKVANSIEKKHPEVEMIVLLVDERPEEVTDMKRSLIGGEVIYSTFD
                     EQPSHHVKVAEMVLARAQRLVEQGKDVVILLDSITRLARAYNLVVPPSGRTLSGGLDP
                     AALYKPKKFFGAARNMEEGGSITILATALIETGSRMDDVIFEEFKGTGNMELHLDRRL
                     SEKRIFPAINLNKSGTRREDLLMTQEEMEAIWYMRRALGNSDPQEVTETIINHLANTR
                     SNAEFISLIKKIKLEA"
     gene            1785..2789
                     /locus_tag="PLM92_04505"
     CDS             1785..2789
                     /locus_tag="PLM92_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009051985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HPR24429.1"
                     /translation="MELKKVFIKDSCPKKVGGQAVLEGIMMKSEDRTSIVLRLPDNSL
                     HIKTEKLPEQKKWTQIPFIRGIFVFVDSLVQGMKTLMYSADVLEEYEGEGALEDDRFS
                     LWLEKKFGKKGAWNIMIYSSVVISVLFTLGVFIIAPTAAVSWLKNFTESEFLLNLAEG
                     LLRIGAFVLYVVLIARMEEIKKVFQYHGAEHKCIHCLETEKELTVENCRKYGTLHPRC
                     GTSFLMFVFVIALVLFSLLGWPDLLFRIISRILLIPVIAGLSFELLKWAGRSDNWVVK
                     ILSIPGLLMQMITTKEPADDQLEVAIAALKAATAKEGEFGQGPCDSQGRIIKEEESII
                     "
     gene            2786..3640
                     /gene="prmC"
                     /locus_tag="PLM92_04510"
     CDS             2786..3640
                     /gene="prmC"
                     /locus_tag="PLM92_04510"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HPR24430.1"
                     /translation="MSLLVRDLVKMGETQLKKVKCPDPKKDAEVLYCYMRNIDRNQFI
                     MEWSEIVDDRICDKYFDLIAERSTRRPLQHITGIQNIMGLDFGIREDVLIPRMDTETL
                     IEKTEEIIKQKGKASVLDLCCGSGAVGISLAVRNKGVKLTMADISSAAKALAEANAKK
                     FGIKTDVLLGDLFAPVKKKYNIIVSNPPYIPSAVIETLEDEVKRFEPKEALDGGVLGL
                     DFYERIIKEAHLHLKKEGWLVMEIGHDQAAQIRTITEETKLYEEYETAKDLAGKTRVF
                     AARIKGKG"
BASE COUNT         1318 a          729 c          945 g          874 t
ORIGIN      
        1 tagccagggc ttatagccct agagcaaacc accctttgga agggtggtta tgattttctt
       61 ttttgtttgc ctccgaaaca atacttgaaa aaacaaggtc tacggtatat agtattgtta
      121 ctggtatttt caaaaggcgg tgttcactgc cttatacata tcgatcgatc tattcccaaa
      181 tatatataaa agtaaataag gatgaattat ggacgaaaaa attttgaaga gtaaaaaagt
      241 tgccgagctg agggaagttg caaaggcttt tggtgtacac gaaagttcca ccttaaaaaa
      301 agaagaactt attgccgccc ttctttcaat ggcagatgaa caaaaatccg gaaagacagc
      361 ggcaaaaaaa gaaatgacag aaacaaaaga ggcagaagca gaaaagtccg gcgcaggatc
      421 cgtgaaggta caaaagaaag ctcccgacaa aaaaggcata agaagaaaag cgtcagaaaa
      481 agccaacggt tttcccgtat ccgccgagga gatgtccgaa agaaaaaatg caaagaccgc
      541 agtacaaaca gagtcagaag aagcagggac aatcaagccg gatgaaaatg aaagaatccg
      601 ggaaaacggc aaggctgaag aaaacagcaa gattgaagca aacggcaagg ctgaagaaaa
      661 cggcaggacc gaagcggaag gcatacttga tatcgcagaa ggcggattcg gatttctcag
      721 gttttcaaac tttctcacca gtgacaagga tgtttatgtt tctccttccc agataagaag
      781 attcaatctc aaaacaggag ataaaatcaa aggaatcaca agagccccga aacccggaga
      841 gaaattcgga gcacttcttt acgtgaacac tgtcaacggt gatgatccgg gagaggcaag
      901 aagaagaccc gactttgatg atcttacccc catctttccg gacgagcgtc tttcccttga
      961 ggacagaagt cgtgatctgg ccatgagagt catagacctg gtggcaccca taggtaaagg
     1021 gcagagagga cttattgtag ctgccccgaa ggcaggaaaa acagttttgc tgaaaaaagt
     1081 ggcaaatagt atagaaaaaa aacatcctga ggtggaaatg atagttctcc ttgtggacga
     1141 aagacccgag gaagtaacgg atatgaaaag atctcttatc ggaggggaag ttatttactc
     1201 aacctttgac gaacagccca gtcatcacgt aaaagtcgca gaaatggttc tggccagagc
     1261 acagcgcctt gtggaacagg gcaaggatgt ggtcatactt ttagacagca taacccgtct
     1321 tgcaagggca tataacctgg ttgttccgcc ttcgggaaga actctttccg gcggtcttga
     1381 ccccgcagcc ctttataaac ccaagaaatt tttcggagct gccagaaaca tggaagaggg
     1441 cggcagcata accatacttg ctacagcact tatagaaacg ggaagccgta tggacgatgt
     1501 catatttgaa gaattcaagg gaacagggaa tatggaactg catcttgaca gaagactatc
     1561 cgaaaagaga atattccccg caataaatct caacaaatcc ggcacaagga gagaagatct
     1621 tcttatgacc caggaagaga tggaagccat ctggtacatg agaagagctc tgggaaacag
     1681 cgatccacag gaagttacgg aaaccataat aaaccatctt gcaaacacaa gaagcaatgc
     1741 agagtttatc agccttatca aaaagataaa gctggaggca tagaatggaa cttaaaaaag
     1801 tttttatcaa agattcctgc ccaaaaaaag tgggcgggca ggctgtactg gaaggcataa
     1861 tgatgaaaag cgaagatcgc acatccatag tgctcagact tcccgataac agtcttcata
     1921 taaaaacaga aaaactaccg gaacagaaaa aatggactca gatacccttt ataagaggta
     1981 tattcgtttt tgttgattct ctggtgcagg gaatgaaaac attgatgtac tcagccgatg
     2041 tactggaaga atacgaagga gagggagctc tggaggatga caggttttct ttgtggcttg
     2101 aaaaaaagtt cggaaaaaaa ggagcctgga atatcatgat atattcttca gttgttattt
     2161 ctgttctttt tactttgggg gtatttatta ttgcacccac agcggcggta agctggctca
     2221 agaattttac ggaaagcgaa ttcctgctta atcttgcgga aggccttttg agaataggag
     2281 cattcgttct ttatgtggta cttatagcaa ggatggaaga aataaaaaaa gttttccagt
     2341 atcacggagc tgaacacaaa tgtatccact gtcttgaaac agagaaagaa ctgactgtag
     2401 agaactgcag aaagtacgga acgctgcatc ccagatgcgg tacaagtttc ctgatgtttg
     2461 tttttgtaat agcacttgtt cttttttctc tgctgggatg gccggatctt ttgttcagga
     2521 taatatcaag gatattgctc atacccgtta tagccggtct ttcttttgaa cttctaaagt
     2581 gggccggaag aagtgataac tgggtcgtga agatattaag cattcccgga cttctgatgc
     2641 agatgataac aacaaaagag cccgctgacg atcagctgga agtagccatt gccgctctca
     2701 aagcagcaac tgccaaagaa ggggaattcg ggcagggacc ctgtgacagt caaggaagaa
     2761 taatcaagga ggaagaaagc attatatgag tttactggta agagatttag taaaaatggg
     2821 cgaaacacaa ttgaagaaag ttaagtgtcc ggatcccaaa aaagatgcgg aggtacttta
     2881 ctgttatatg cggaatattg acaggaatca gtttataatg gaatggtcgg aaatagtgga
     2941 tgaccgcatt tgcgacaagt atttcgatct tattgcagag agaagtacca gaagacccct
     3001 tcagcatata acaggaatac agaatataat gggactggat tttggtataa gagaggatgt
     3061 ccttatcccg cgcatggaca cggagactct gatagaaaaa acggaggaaa taataaaaca
     3121 gaaaggaaaa gcatctgtac ttgatctttg ctgcggcagc ggtgcggtgg gcatttccct
     3181 tgcggtacgc aacaaaggtg taaagcttac catggcggat attagttctg cggcaaaagc
     3241 actggctgaa gcaaatgcaa agaaattcgg aataaaaacc gatgtacttc tcggagatct
     3301 ttttgctccg gtaaaaaaga agtataatat catagtttca aatccgccct acataccttc
     3361 tgctgtgata gagacccttg aagatgaagt gaaaaggttc gagccaaaag aagccttgga
     3421 cggaggagtc cttggtctgg atttttatga aaggatcata aaagaagcgc atttacatct
     3481 gaaaaaagaa ggatggctgg tgatggagat aggacatgat caggcggcac agatacggac
     3541 aataaccgag gaaacaaaac tgtatgaaga atatgaaaca gccaaggatc tggccggcaa
     3601 aaccagagtt ttcgctgcaa ggataaaagg gaaaggataa gtaacagcct gatcggctcc
     3661 ggcccaagcc aaaggcaaaa ggcaaaactt gaagatcagc ctgatcggct ccggcccaag
     3721 ccaaaggcca aatgcaaaac ttgaagatca gcctgataaa cttaagccaa atgcaaaact
     3781 tgaagatcag cctgataaac ttaagccaaa gggtcaagtc catttttgtg tgtaaattcg
     3841 ttcggtcgtc atctgcaggc gagggc
//
LOCUS       DAONHK010000103         3858 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_18790_length_3858_cov_4.515646, whole genome shotgun sequence.
ACCESSION   DAONHK010000103 DAONHK010000000
VERSION     DAONHK010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3858
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18790_length_3858_cov_4.515646"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..622)
                     /gene="tsaD"
                     /locus_tag="PLM92_04515"
     CDS             complement(<1..622)
                     /gene="tsaD"
                     /locus_tag="PLM92_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014966492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HPR24431.1"
                     /translation="MLDGKHLTLAIETSCDETSAALMAEGRQVLSNVISSQIDIHKAF
                     GGVVPEIASRHHLTNINHVIKRTFDEAGASMKDVDLIGVTKGPGLVGALLVGIATAKA
                     FALSTGKPLVGVHHIKGHLSANYIAHPDLGPPFTALVVSGGHTELIRVNGYDSLKYIG
                     GTRDDAAGEAYDKVARVLGLGYPGGPLIDEAAKKGDPDAIGFKRVYL"
     gene            complement(616..1101)
                     /gene="rimI"
                     /locus_tag="PLM92_04520"
     CDS             complement(616..1101)
                     /gene="rimI"
                     /locus_tag="PLM92_04520"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008423560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HPR24432.1"
                     /translation="MKGFIIETAKENDVDELADLDALCFEHPWSRDSFERELGHNKLA
                     FYLAAKLPEKEGGKIIGYAGIWLIIDEGHITNIAVHPDFRQKHIASSMMEFLLKETSK
                     AGIKRFTLEVRKNNTAARRFYKKYGFTELGIRSGYYENNEDAYIMWLDTEEGKDREDK
                     C"
     gene            complement(1098..1931)
                     /gene="tsaB"
                     /locus_tag="PLM92_04525"
     CDS             complement(1098..1931)
                     /gene="tsaB"
                     /locus_tag="PLM92_04525"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HPR24433.1"
                     /translation="MYILAVETTGPFCSAAVIDEKQNINEEVSREKMSHLKNLMPLVK
                     KVLEERKITSHGQKRALQLDDIDAIAVSCGPGSFTGIRIGVSSARALSQAEGGKPLIP
                     VPTLITFAHGEKEHKGLICPVLDARRNQVYAGAWQWEQEQGKIKSEEDFLFEGADFPK
                     HLCTKNIRERVPAGPYMLEEFLELMEVYDDIMFFGDGLLPYGEKIKKWAHTEDRKISF
                     APSENRFQSAALVAKHGLMLYKEGKTVGYEELMPNYMRKAEAQKRLEEGTLSKRIKGE
                     I"
     gene            complement(1951..2415)
                     /gene="tsaE"
                     /locus_tag="PLM92_04530"
     CDS             complement(1951..2415)
                     /gene="tsaE"
                     /locus_tag="PLM92_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006001571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HPR24434.1"
                     /translation="MKHTEKEIKMEKEIKLKNEAEVREIGIKLGQKLLPGSILALEGE
                     LGTGKTSLTRYIGLGLGITRNIKSPTFTILNEYRGGRLPLYHFDVYRIDDPKEMEELG
                     YEEYFFGEGITVLEWADKIKALLPDDAMIIRMGYGKGEEERVYRFNFDIDSI"
     gene            complement(2515..3066)
                     /locus_tag="PLM92_04535"
     CDS             complement(2515..3066)
                     /locus_tag="PLM92_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HPR24435.1"
                     /translation="MYENLNTQKKTVKLAKMGMLVAISVVLVYLIHFPIFPAVPFLEY
                     DPADIPIFIGTFAFGPLGGLAITVVTAVVQGTTVSAASGVYGILMHVIATGSFVLVAG
                     IIYKRNKTRKGAVIALAAGSLTWIVAMFFANLIITPYFMGVPRAVVMDLMPFIVAFNA
                     IKAGGNSLITFLLYKRISAFLHK"
     regulatory      complement(3167..3305)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(3409..>3858)
                     /locus_tag="PLM92_04540"
     CDS             complement(3409..>3858)
                     /locus_tag="PLM92_04540"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HPR24436.1"
                     /translation="EPAGNLTVNVGKIEMDTRAVRLSMNVRYPVTQSDKKVYEGIAPV
                     IERYDLGIIKKLHHEPLYRSLDDPMVKTLLAVYREHTGDTESAPKVIGGGTYARAMDN
                     CIAFGASFPGQRDIAHQKDEFINASDMIKMTNIFADAIYRLSKSGIM"
BASE COUNT          882 a          991 c          758 g         1227 t
ORIGIN      
        1 caagatatac ccgcttgaat cctatggcat cgggatctcc ttttttcgcc gcctcatcta
       61 taaggggtcc tccgggatat cccagtccca aaacccttgc caccttgtca taggcctctc
      121 ccgccgcatc atccctggtg ccgccgatgt actttaatga atcgtatccg tttaccctta
      181 taagttccgt atggccccct gatacaacaa gagctgtaaa gggaggtcca agatccggat
      241 gggctatata attggcactc agatgcccct ttatatgatg tactcccacc agaggttttc
      301 ctgtggaaag agcaaaagcc ttggccgttg cgatccccac caggagagct cccacaagcc
      361 ctggtccctt ggtgactcct ataagatcca catctttcat ggatgctccg gcctcatcaa
      421 aggtcctttt tataacatgg tttatattgg tgagatggtg ccttgaagct atttcgggca
      481 ccactccccc aaaggcctta tgtatgtcta tctgggatga gatcacatta gataaaacct
      541 gacgcccctc tgccataagg gcggccgagg tttcgtcaca gcttgtttca attgccagcg
      601 tcagatgctt tccgtctaac atttgtcctc cctgtctttt ccctcttcag tatccagcca
      661 cattatatat gcgtcttcat tgttctcgta gtatccggac cggataccca gttctgtgaa
      721 accgtatttt ttatagaacc tgcgggcagc cgtgttgttt ttcctgactt ccagtgtaaa
      781 tctttttatt cccgccttag atgtttcttt cagcaaaaac tccatcatag atgaagctat
      841 atgcttttgt ctgaagtccg gatgtacagc aatgtttgtt atatggccct catctattat
      901 cagccagata cctgcgtatc ctattatttt ccctccctct ttttcgggaa gttttgccgc
      961 aagataaaag gccagcttgt tatggccaag ctccctctca aaagaatctc tgctccaggg
     1021 atgctcaaaa cataaagcat caagatccgc cagttcatcc acatcatttt cttttgcggt
     1081 ctcaattatg aatcctttca tatctcgcct tttattcttt tgctcaatgt accctcttca
     1141 agtctttttt gtgcttccgc ctttctcata taattgggca tgagctcttc atatccgacg
     1201 gtttttcctt ctttgtaaag cataagaccg tgctttgcca caagcgccgc ggactggaac
     1261 ctgttttcgg agggtgcaaa agatattttt ctgtcttccg tatgtgccca tttttttatc
     1321 ttctccccat agggcaaaag tccgtcaccg aaaaacatta tgtcatcgta tacttccatg
     1381 agctccagaa attcttcaag catatatggc cccgccggaa ctctttcccg tatatttttt
     1441 gtgcagagat gtttcgggaa gtcagctcct tcaaagagga aatcttcttc gctctttatt
     1501 tttccctgtt cttgttccca ctgccatgcg ccggcatata cctgatttct tctggcatcc
     1561 agcacagggc atataaggcc tttgtgctcc ttttccccat gggcaaaagt aataagtgtg
     1621 ggcacgggga tcaatggctt tcccccttca gcctgggaca gggctcttgc ggaagagaca
     1681 cctatcctta tacctgtaaa tgagccgggg ccacaggata ctgcgatggc atcgatatcg
     1741 tctaattgca atgcacgttt ttgcccatgg ctcgttatct ttctctcttc caggaccttt
     1801 tttacaagag gcatgagatt tttcagatga ctcatcttct cccgggaaac ctcttcattt
     1861 atattctgtt tttcgtcgat cacagctgcc gaacaaaacg gtccggtggt ttcaacggct
     1921 aaaatataca tatctttcct ccggtaagca tcatatactg tcaatatcaa aattgaagcg
     1981 gtatacccgt tcttcttcac ccttgccgta ccccatgcgg atgatcatgg catcatcggg
     2041 cagcagcgcc tttatcttat cggcccattc caaaacggtt ataccttctc cgaaaaagta
     2101 ttcttcatag cccagttctt ccatttcttt gggatcgtct atcctgtaaa catcaaaatg
     2161 ataaagcggc agtctgcctc cccggtattc attcaaaata gtgaatgtgg gacttttgat
     2221 attccgggtt atcccaagac ccagcccaat atagcgggtc agtgaagttt tgcccgtacc
     2281 cagttcccct tcaagggcaa gtatgcttcc cggaaggagt ttctgcccca gctttatgcc
     2341 gatttcacga acttccgctt cattttttaa ttttatctct ttttccattt tgatttcttt
     2401 ttcagtgtgt ttcatatgat ttgattttta tccacgcaaa atattctaac ataaaaagag
     2461 cgcatttgga aatcctttgc ctgcgctctt tcataagttt gcttttcttt ctttttattt
     2521 gtgcaggaat gccgatatcc ttttatagag caggaatgta ataagcgaat tacctccggc
     2581 ctttatggcg ttgaatgcca ctataaacgg catcaggtcc atcaccactg ctcttgggac
     2641 tcccatgaaa taaggggtta ttataaggtt tgcaaaaaac atggctacta tccacgttaa
     2701 actgcctgca gcaagggcga tgaccgcgcc cttccttgtc ttatttcttt tgtaaatgat
     2761 acctgccaca agaacgaagg atcccgttgc aattacatgc ataagtatcc cgtatactcc
     2821 gctggccgca cttaccgtgg taccctggac cacggcggta acaacagtaa tcgccagacc
     2881 tcccaggggt ccgaaggcga atgttccaat aaagatcggt atatccgccg gatcatattc
     2941 caggaaaggt accgccggga atatgggaaa atggatcaga taaacaagta ccaccgaaat
     3001 ggccaccagc atacccattt tggcaagctt tactgttttt ttctgggtgt taagattttc
     3061 gtacattttt ttgattcctt tctttttgtt ttgttgatct ggaatctgtt cagctgcgta
     3121 aaaaacgccc cggatgttcc cggggcgcag gcataaatac aaagtattca cgtttctttc
     3181 atccggacta taccgtcggt accgggatct taccggttcg gcctttcggc tcgcggactc
     3241 gtagcgatgc ttttgcaatg ctctttaccg ccggtgagga attccacctc gccctgaaac
     3301 agatctttta tatattactt attctatatc ttttaatgat atcttgtcaa tatgttcccg
     3361 atttttcaga ctttttgaaa ttcagtccca cagcggtcct ttgattcttt acattatccc
     3421 gcttttgctc agtctgtata tggcatcggc aaaaatatta gtcatcttta tcatgtccga
     3481 agcgtttatg aattcatctt tttgatgggc tatgtccctt tgtccgggga aacttgcacc
     3541 aaaggcgata cagttatcca tggctcttgc ataggtccct cccccgatca ccttaggggc
     3601 gctttccgta tctcctgtat gttccctgta aacggcaaga agcgtcttaa ccatgggatc
     3661 gtcaagggat ctgtaaagag gttcgtggtg cagctttttt attatcccca gatcatatct
     3721 ttctatgaca ggtgctatgc cttcatatac ttttttatcg ctttgggtta ccggatacct
     3781 cacattcatt gaaagacgta cggctctggt gtccatctct atcttcccca cgttcactgt
     3841 caagttgccc gcaggctc
//
LOCUS       DAONHK010000104         3847 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_18860_length_3847_cov_4.933808, whole genome shotgun sequence.
ACCESSION   DAONHK010000104 DAONHK010000000
VERSION     DAONHK010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3847
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18860_length_3847_cov_4.933808"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            250..1575
                     /gene="rsxC"
                     /locus_tag="PLM92_04545"
     CDS             250..1575
                     /gene="rsxC"
                     /locus_tag="PLM92_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009643745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HPR24437.1"
                     /translation="MFFKGKSLKGIKVDHNKTTAGAETERMIPVPAYVYISMSQHIGA
                     PCKPLLGPGEMVRVGQLIADSDAFVSVPIHSSVSGTVEKIDGIRSSTGGEDKVMVIKT
                     DRKQDIVEGIKPPRIRSREGFVKEVRASGLVGLGGAAFPTHIKFSPKNPDEVKTLIIN
                     GAECEPFITSDYRTMIEDTDDIISGIKLTMKYLEMEECIIGIESNKPDAIELIKEKLD
                     KQGLDNIKVITLKTSYPQGAERVLVEVITGKAMAAGVLPAELGVIVSNITTIAFLGQY
                     FRTGMPLISKRITVDGSAVRQPKNIRAPIGAMISDVIDFCGGYRGKPSKIIMGGPMMG
                     RAIFSDNLPIVKNNNAILAFTGRQAYIEEETACINCGRCFRACPINLLPYALCRAYDR
                     KDIERLKELDVMQCMECGSCAYVCPARKPLGFTNKIGKQLVKEASENDR"
     gene            1565..2566
                     /locus_tag="PLM92_04550"
     CDS             1565..2566
                     /locus_tag="PLM92_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006598266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HPR24438.1"
                     /translation="MIGSMKNKNNLKLIVSASPHLVSGLNTQKIMLYVIIALMPSLAA
                     STWIFGFRAFALTVFCVAACMIFEYVFSLIAKKPQTTKDLSAVVTGILLAFNLPVSLP
                     YWMAAVGCFIAIIIVKQLFGGIGKNIVNPAITARVVLLVSFPAAMTTWQTTRFAGDAL
                     TGATPLGLIKEGMEVPANIDLFLGNVGGSMGEVSALALLIGFAFLLIKKVVSSIIPLS
                     YLAVVFFFALAVGEDPIFHIFAGGVMLGALFMATDYVTTPVLPPGQIIFGLGCGFITM
                     AIRIWGAYPEGVSFSILFMNILVPHIDRLCDYIIFRVKASKKKAKKALIDNTVEGTK"
     gene            2563..3042
                     /locus_tag="PLM92_04555"
     CDS             2563..3042
                     /locus_tag="PLM92_04555"
                     /inference="COORDINATES: protein motif:HMM:NF016121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HPR24439.1"
                     /translation="MNFKRRLGDYLLPTIVLVVICLVVTCALAFTYSKTKTTIDANAV
                     KTQDEAAKEVLGDEEGKALEETVQSYGGEMSVMVGISKEGKITGVKVISHGDTPGLGT
                     KAMEPEYLKQYKGTEKLEQESIREDDSKDAITGATISSNAVYVAVKKALLEYKKAGR"
     gene            3047..3724
                     /locus_tag="PLM92_04560"
     CDS             3047..3724
                     /locus_tag="PLM92_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009321778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HPR24440.1"
                     /translation="MKDKLSIFTKGIIKENPVLVLLLGMCPTLAVTTSAINGLGMGVS
                     VIVVLTCSNIVISLLKKAIPGKVRVPGYIVVIAGFVTVVQLLLKAYLPELDESLGLFI
                     PLIVVNCIILGRAEMFASKNTIIDSMLDGLGMGLGFTMALFLIGLIREFFGAGTAFGI
                     ELMADITPMGIFMLAPGGFFVLAMIIAAINWLSKGRAIKKKEIGCDGCPMISTCSGIC
                     EKEEAGV"
     gene            3726..>3847
                     /locus_tag="PLM92_04565"
     CDS             3726..>3847
                     /locus_tag="PLM92_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019678778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HPR24441.1"
                     /translation="MEQIIIIMMSVILVDNFVLSQFLGICPFMGVSKKLDSARG"
BASE COUNT         1193 a          710 c          960 g          984 t
ORIGIN      
        1 gtagccaggg cttatagccc tagagcaaac caccctttgg aagggtggtt atgatatgga
       61 taagcatgaa aaagtataca aaatacatat tgaaagatac tgtaaaaaga taaacagttg
      121 agttacaata catctggtga aggattagtt tgcacatact ataaactaat ttgcgtttta
      181 tccggctgct gtcagagaag gcggcctgtg gaatattatg aagcaaatag agaaaggacg
      241 aaaaaaacag tgttttttaa agggaaaagt ctaaaaggca taaaggtgga tcataataaa
      301 acaactgctg gggctgagac cgagagaatg ataccggtac ctgcttatgt ttatatatcc
      361 atgtcacagc acataggggc accttgcaag cctcttttgg gccccggaga gatggtaaga
      421 gtgggacagc ttattgcaga ttcagatgca tttgtaagtg tgccaataca ctccagcgta
      481 tcgggaactg ttgagaaaat cgatggcata cgttcgtcca ccggcgggga agacaaggta
      541 atggtcataa aaacagacag aaaacaggat attgttgaag gtataaaacc tcccaggata
      601 agaagcagag aaggctttgt caaagaggtc agggccagcg gtcttgtagg tcttggcggt
      661 gcggctttcc caactcatat taagttttct cccaaaaacc cggatgaagt aaaaactctg
      721 ataataaacg gagccgagtg cgaacctttt ataacctccg attacagaac tatgatagaa
      781 gacacggatg acataataag cgggataaag ctcacaatga agtatcttga gatggaggag
      841 tgtattattg gcatagaaag caacaagccc gatgccatag agcttatcaa agaaaaactc
      901 gataaacagg gccttgataa tatcaaggtg attacactga aaacctccta tccacagggt
      961 gcagagagag tcctggtgga agtaataaca ggaaaagcca tggccgcggg agtccttccg
     1021 gcggaacttg gtgtgatagt aagcaatata acgaccattg cattcctggg tcagtatttc
     1081 aggacaggca tgcctctgat atcaaaaagg ataacagtag atggatctgc tgtaagacaa
     1141 ccgaagaata tacgggcccc cattggtgcc atgatatctg atgtgatcga tttctgtgga
     1201 ggatacagag gcaagccaag caagataata atggggggac ccatgatggg aagggctatt
     1261 ttttcagaca acctgccgat agtcaaaaac aataatgcaa tactggcttt tacaggcagg
     1321 caggcgtata tcgaagaaga aactgcatgt ataaactgcg gccgctgctt tagggcatgt
     1381 ccgataaatc ttttgcccta tgccctttgc agagcttatg acagaaagga tatagaaaga
     1441 ctgaaagaac tcgatgtcat gcagtgtatg gaatgcggca gttgcgcata tgtatgcccg
     1501 gcaagaaagc ctctgggatt caccaataaa ataggcaaac aactggtaaa ggaggcgtct
     1561 gaaaatgatc ggtagtatga aaaacaaaaa taatctcaag cttatcgttt cggcatcgcc
     1621 tcatctggtt tccggactta atactcagaa gataatgctt tatgttataa tcgcccttat
     1681 gccgtccctg gcggcaagta catggatatt tggattcagg gcatttgcgc ttacagtttt
     1741 ctgcgttgcg gcatgcatga tttttgaata tgttttcagc cttatagcaa aaaaaccaca
     1801 gacaacaaag gatctttccg cagtggtgac aggtatactt ctggcattca atctgccggt
     1861 gtcactgcct tactggatgg ctgcggtggg ttgttttatc gccataataa tagtaaagca
     1921 gttattcggc ggtataggca agaatatcgt aaatcctgca atcacggcca gagttgtttt
     1981 gcttgtatcg ttccctgcag caatgaccac atggcagacc accagattcg ccggcgatgc
     2041 acttaccggt gcgactccct taggtcttat caaagaggga atggaagtgc ctgccaatat
     2101 tgatcttttc cttggcaacg taggcggatc catgggtgag gtaagtgccc tggcgctgct
     2161 tataggcttt gcttttcttc ttataaagaa agtggtttca tctataatac ccttgagtta
     2221 cctggctgta gttttctttt ttgctcttgc ggtgggagaa gatccgattt ttcacatttt
     2281 tgcgggaggt gtgatgctgg gagccctgtt tatggccact gattatgtga caacaccggt
     2341 gcttcccccc gggcagatca tcttcggtct gggatgtgga tttataacta tggccataag
     2401 gatatgggga gcatatcctg aaggagtctc gttttctata ctctttatga atatacttgt
     2461 accgcatata gacagattat gtgattatat aatattcaga gtaaaggcat caaagaaaaa
     2521 agcaaaaaaa gctttgatcg ataatacagt ggagggcaca aaatgaattt caaaagaaga
     2581 ctgggagatt atttactgcc cacaatagtg cttgtagtca tatgtcttgt tgtgacctgt
     2641 gcactggctt tcacatattc aaaaacaaaa acgaccatag atgccaatgc agtcaagact
     2701 caggatgaag ctgcaaaaga agtccttggg gatgaagaag ggaaagcact ggaagaaacc
     2761 gttcaaagtt atggaggaga aatgtctgtt atggtgggca taagtaagga aggaaagata
     2821 acgggagtaa aagtgatttc tcacggagac acaccgggac ttggaacaaa ggctatggaa
     2881 cccgagtatc tgaaacagta taaaggaaca gaaaaactgg agcaggaaag cataagagag
     2941 gatgacagca aagatgccat aaccggagcc accatatctt cgaatgctgt atatgtggca
     3001 gtaaaaaaag ctcttttaga atataaaaag gcgggacggt gatgaaatga aagacaaatt
     3061 aagcattttt acaaaaggaa taataaaaga aaaccctgtt ctggtacttt tgcttggaat
     3121 gtgcccgact ctggccgtta ccaccagtgc cataaacgga cttgggatgg gagtctcggt
     3181 aatagttgtt ttgacatgtt caaatatcgt gatatcgctg ctcaaaaaag ccatccccgg
     3241 gaaagtaaga gtccccgggt atatagtagt tatcgccggt ttcgttacag tggtgcaatt
     3301 gcttttaaaa gcgtaccttc cggagcttga tgaatctctg ggactgttca ttccactaat
     3361 agtcgtaaac tgcataatat tgggaagagc agagatgttc gccagtaaga atacaataat
     3421 cgattccatg ctggatggtc ttggcatggg tctcggattc acaatggcac tttttcttat
     3481 cggactgata agagagtttt ttggagccgg aacagcattc ggaatcgagc tgatggctga
     3541 tataacgccc atgggcatat tcatgctggc accgggagga ttctttgtac ttgccatgat
     3601 aatagctgca ataaactggc tttcaaaggg aagagcgata aaaaagaagg aaatcggctg
     3661 cgacggatgc cctatgataa gtacctgcag cggtatatgt gaaaaagagg aggcgggggt
     3721 ataaaatgga acagattatc attattatga tgagtgtaat actggtggac aattttgtat
     3781 tatcgcagtt tctgggcata tgtcccttta tgggagtatc gaaaaaactg gactccgcca
     3841 gagggat
//
LOCUS       DAONHK010000105         3752 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_19520_length_3752_cov_3.918042, whole genome shotgun sequence.
ACCESSION   DAONHK010000105 DAONHK010000000
VERSION     DAONHK010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3752)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3752)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3752
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19520_length_3752_cov_3.918042"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(105..1052)
                     /locus_tag="PLM92_04570"
     CDS             complement(105..1052)
                     /locus_tag="PLM92_04570"
                     /inference="COORDINATES: protein motif:HMM:NF036772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HPR24442.1"
                     /translation="MQKPKKLMPGATIGVISPSSPSETRSEVPRAVEYLSNMGYNIIT
                     GRNLNKTKGFVAASEEDRAADLNEMFKRQDIDAVFVTQGGYGAAQLLDLIDFEMIKKN
                     PKIFTGFSDITSLHLMLNKFCDLVTFHGPGMARFNEEDLTEYTRKQFFDALCIDKPLG
                     KINTADKKKWLYKISGGKTEGQITGGNLTLVCASLGTPYEIDCRGKILFLEDVDTEPW
                     IIDHNMCHLRNSGKLNDALGFVIGECKNCRPYDYKPGYNCDISLEEVLEYYLKDLSKP
                     VLYGLPLGHTDDIATIPLGVSVLLDGDSKTLEIKENGVL"
     gene            complement(1219..1614)
                     /locus_tag="PLM92_04575"
     CDS             complement(1219..1614)
                     /locus_tag="PLM92_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HPR24443.1"
                     /translation="MYNHVMAVVNIDETQKMLDEIAEKLPKEFFKELNGGILLLPEIK
                     RSPHAKKNDLYILGEYMKSSSMGRMIYIYYGSFQRLFGNMEREDFRRRLKETLLHEFT
                     HHLESLAGERGLEKKDEEKLIRYMENKKI"
     gene            1717..1914
                     /locus_tag="PLM92_04580"
     CDS             1717..1914
                     /locus_tag="PLM92_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPR24444.1"
                     /translation="MPLILLGLLVIVVTVAYFYLNGMMKPDSESKKKKKEDPSKIIYL
                     KKEDWKENTSEDNKNDNDGSK"
     gene            complement(1999..>3752)
                     /gene="pnp"
                     /locus_tag="PLM92_04585"
     CDS             complement(1999..>3752)
                     /gene="pnp"
                     /locus_tag="PLM92_04585"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013297884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HPR24445.1"
                     /translation="TVMCMDNDAPSEIAAMIGSSVALSVSDIPWEGPTGSVLVGRIDG
                     EFVINPTSAEREKSDMHMVVSGTKEAIMMVEAGAEEVAESDMLDGIMFAHEEIKKIVA
                     FIEEVVEEVGKAKKEIECYKVPEDIENDVREYAEEKMRAAVLTVEKQERLDNMDAVEV
                     ETQEHFAEKYPEGEKDIANILYTITKEQVRRLILDDCIRPDNRKHEEIRPIWVETGVL
                     PRCHGTGLFKRGQTQALSVATLGPVGEGQRLDGISEETEKRYMHHYNFPAYSVGETKP
                     MRSPGRREIGHGALAERAIVPVLPEVEEFPYAIRVVSEILSSNGSTSMASTCGSCLAL
                     MDAGVPIKAPVAGIAMGLIERVEEDGSSKMAILSDIQGMEDFLGDMDFKVAGTEKGVT
                     AIQMDIKVHGLSREILEKALSQAKEGRMHILKEMMSEIKEPREELSPWAPRIISLQID
                     PDKIRTVIGPGGKTINGIIDKTGVKIDIDDDGLVYIAAPDMESAEKGLNEIEMLIKEV
                     EVGEVYKGKVVRIMNFGAFIEILPGKDGLLHISKMAKHRVEKVEDIMNIGDEVEVKVT
                     EIDSQGRINLLRTQLPD"
BASE COUNT          925 a          918 c          664 g         1245 t
ORIGIN      
        1 ttagcagaaa cattattctc atggcctcat tttttcatat ctactttaca gacccaaagt
       61 aactgttatg ttatttatga attattgctg atttgcatat cgcactacaa aacaccgttc
      121 tccttgatct cgagcgtttt ggaatcaccg tcaagcaata cggatactcc aaggggaatg
      181 gtggcaatat catctgtatg tccgagaggc aagccgtaaa gtaccggttt tgaaagatcc
      241 ttcagataat actcaagaac ttcctccaga cttatgtcac agttgtatcc gggtttatag
      301 tcgtaaggtc tgcagttttt gcactctcct atgacaaatc caagggcatc gttgagtttt
      361 ccagagttcc tcaggtgaca catattatga tcaattatcc acggttcagt atcaacatcc
      421 tcaagaaaca gtattttgcc tctgcagtct atttcatagg gtgttcccaa acttgcacat
      481 accagggtca gattacctcc ggttatctgt ccttctgttt tgccgccgct tattttataa
      541 agccatttct ttttgtccgc cgtatttatc ttccccagag gcttatcaat gcataaggca
      601 tcaaaaaact gtttcctggt atattccgta agatcttctt cattgaatct tgccatgccg
      661 ggtccgtgaa atgttacgag gtcgcaaaat ttattaagca taagatgtaa agaagtaata
      721 tcgctgaatc ccgtaaatat tttgggattc ttttttatca tttcaaaatc aataaggtca
      781 agcagttgtg ctgcaccgta tccgccctgt gtaacaaaaa cggcatctat atcctgccgt
      841 ttgaacatct cattcagatc ggccgctctg tcttcctcgg acgcagcaac aaaacctttc
      901 gtcttattaa ggttcctgcc ggttatgata ttatatccca tgttcgaaag gtattcgact
      961 gcccttggca cctcacttct tgtttcagaa gggctcgaag gcgaaatgac acctatggtg
     1021 gctcccggca ttaatttttt gggtttttgc atggcgcacc tcatcataca taatactttt
     1081 ttacttaata gacttatctg ttttaatgtg tttataatat gaacgcttat ttctatttat
     1141 gaacatcatt attatatcac ctatgctccg ggccgtcaat aaaaaaagac agataattct
     1201 gtcttttagc attgcaggtc atattttttt gttttccata tatctgatca gtttttcttc
     1261 atccttcttt tccagccctc tttccccggc cagagattca agatggtgtg taaattcatg
     1321 aaggagcgtt tcttttaacc tcctccggaa atcctctctt tccatatttc cgaacagtct
     1381 ttgaaaggat ccgtaatata tgtatatcat tcttcccatg gaactgcttt tcatatactc
     1441 cccgagtata taaagatcgt tttttttggc atggggactt cttttgattt ccggcagcaa
     1501 aagtatccct ccgttcagtt ctttaaaaaa ctcttttgga agtttctctg cgatttcatc
     1561 cagcattttc tgcgtttcat caatatttac aactgccata acatgattat acattttatt
     1621 accgcccatt tcaaagaaaa gaccaataat cacaacctgc acttttcatt atgtgtgact
     1681 ttgttgtata ataagaaaaa ggaaggaggt gcaaatatgc cgcttatatt gttaggtctg
     1741 ctcgtaatag tcgttactgt tgcatatttt tacttgaacg gcatgatgaa gcccgattcc
     1801 gagagcaaaa agaaaaaaaa ggaagatcct tcaaaaataa tatatctcaa aaaagaggac
     1861 tggaaggaaa acacctccga agacaacaaa aacgataacg acggatcaaa atgaataaat
     1921 aaaaatgtgc attgacccgg aaaaggatcc ctgcacattt tttatttgtt ctctgatatg
     1981 gaaatcaact gcggtatatc agtcaggcag ctgtgtcctt aaaaggttga tccttccttg
     2041 actgtctatc tcggtgactt tcacttccac ttcatctccg atattcatta tatcttcaac
     2101 tttttcgacc ctgtgtttcg ccatctttga tatgtgcagc agcccgtctt ttccgggcag
     2161 gatctctata aaggctccaa aattcattat tcttaccact ttgcccttat aaacttctcc
     2221 gacttccact tcttttataa gcatctctat ttcattaaga cctttttccg cactttccat
     2281 atccggtgcc gcaatataaa caagcccgtc atcatcaatg tctatcttga ctcctgtttt
     2341 gtctattatg ccgtttatag tcttccctcc cggacctatg actgttctga tcttgtcagg
     2401 atctatctgc aaagaaatta tcctgggtgc ccagggagaa agttcctctc tgggttcttt
     2461 gatctcactc atcatttctt tgagtatatg catcctgcct tccttcgcct gagacagtgc
     2521 tttttcaagg atctctcttg aaagcccgtg cacttttatg tccatctgta tggctgtaac
     2581 acctttttcg gtcccggcca ccttgaagtc catatcgccc aggaaatctt ccattccctg
     2641 tatgtccgac aatatggcca ttttgctgct gccatcttct tcaactcttt caataagccc
     2701 catggctatg cctgccacag gcgcttttat tggcacgccc gcatccatca gggcaagaca
     2761 actgccacat gtggatgcca tggatgtgga tccgttcgag gaaagtattt ctgacactac
     2821 tcttattgca taaggaaatt cctccacttc gggaagcacc ggtacaattg ctctttccgc
     2881 cagcgctccg tgtcctatct ctcttctgcc cggacttcgc atgggttttg tttcccccac
     2941 tgaataagcc ggaaaattat aatgatgcat atatcttttt tcagtttctt cggatatgcc
     3001 atccagtctc tgaccttccc caacgggacc aagcgtggct acagaaagag cctgtgtctg
     3061 tccccttttg aagagtccgg tgccgtgaca tcttggcagg acgccggttt ccacccatat
     3121 gggtcttatt tcttcatgct tcctgttgtc aggtcttatg cagtcatcaa gtataagtct
     3181 tctgacctgc tcttttgtta ttgtataaag tatattggcg atatcctttt cgccctccgg
     3241 atatttctcc gcgaagtgtt cctgagtctc gacttccact gcatccatgt tatccagtct
     3301 ttcctgtttt tctacagtaa gtacagcagc cctcatcttt tcctcagcat attccctgac
     3361 atcattttct atatcttcag gaaccttgta gcattcgatc tctttttttg ccttgccgac
     3421 ttcttctact acttcttcaa tgaatgcaac tattttcttg atttcttcat gggcaaacat
     3481 tatgccgtca agcatatcgg attcagcgac ttcctccgca cctgcctcta ccatcatgat
     3541 cgcttctttt gtgcccgaaa ccaccatatg catgtcgctt ttttctctct cggccgatgt
     3601 ggggttgata acgaactctc cgtcgatcct gcccacaagt accgaacccg tgggtccttc
     3661 ccaaggaatg tccgaaacgg acaatgccac agatgagcct atcattgcag ctatttccga
     3721 gggggcatca ttgtccatac acataacagt gg
//
LOCUS       DAONHK010000106         3708 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_19866_length_3708_cov_4.448125, whole genome shotgun sequence.
ACCESSION   DAONHK010000106 DAONHK010000000
VERSION     DAONHK010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3708
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19866_length_3708_cov_4.448125"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..832
                     /gene="hydG"
                     /locus_tag="PLM92_04590"
     CDS             <1..832
                     /gene="hydG"
                     /locus_tag="PLM92_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005929422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HPR24446.1"
                     /translation="YILFQETYNKERYLELHPSGPKRDYDYHTEAMDRANEGGIDDVG
                     CGVLFGLDNYKYDFVGILMHAKHLEDTWGCGPHTISVPRIRPADDIDVSDFQNGVPDD
                     IFKRIVAVLRIAVPYTGMIMSTRESEKTRRECLEIGITQVSGGSRTSVGGYEEEERDE
                     STAQFDVSDTRTLDQVVSWLIELGYVPSFCTACYREGRTGDRFMSLLKSGQIANCCQP
                     NALMTLKEYLVDYASPETYEKGEAIIQKRLAMVPNEKVREIAKKHLKEIENGKRDFRF
                     "
     gene            832..2034
                     /gene="hydF"
                     /locus_tag="PLM92_04595"
     CDS             832..2034
                     /gene="hydF"
                     /locus_tag="PLM92_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006355632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HPR24447.1"
                     /translation="MNSLNETPRAGRLHISIFGKRNSGKSSLINALTGQDIAIVSEHA
                     GTTTDPVYKSMELHPVGPVVFIDTAGFDDFGKLGELRLSRTKEVIKKTDIALLLFDDG
                     SLDPEEGWLSGLRDADVPVIPIINKTDLLDRAALQQKIIMVFGLTPIMVSAADGTGIE
                     DIKKAIDKLAPEDFKREKILGDMVSAGETVLLVMPQDIQAPKGRLILPQVQVIRELLD
                     NKCTVVSTVSDGFLKALDSLSRAPSLIITDSQCFGYVNKNKPGESALTSFSILLAAFK
                     GDLKTFVEGAAAIDSLTEKSRVLIAEACTHAPLEEDIGREQIPGMLRERAGEGMRVDI
                     VAGSDFPKDLKKYDLIIHCGSCMFNRKHMMSRVEQAKTQGIPITNYGIVMAKLKGILD
                     DITLPPGR"
     gene            complement(2321..3706)
                     /locus_tag="PLM92_04600"
     CDS             complement(2321..3706)
                     /locus_tag="PLM92_04600"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008691878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HPR24448.1"
                     /translation="MYAFCQNLVAVDLSNIRSIKEMISVMKNKSKEIPEGCWIKGVNF
                     DDTLFKEKRMPTRHDLDNVSNKHPIVIRRVCLHAIVVNTEGLTLAGVIKGYNPEGGGI
                     VETEADGNPNGILREQCTQVIDNIIKDPLSDIALREKIMMKVFKDMSSRGITGIHTYS
                     AKIWNYKESIETYKHLENIGLLPVRVYVCLDELFDATTKINNDPNAKVHLGSYKLFTD
                     GSLGAESAALRDPYKNSGDSGVMICTEDELYNKMKTAYDKGLQPAIHAIGDRALEITI
                     NAIEKTILSSKTKPALPFRIIHAQMTPPDLIEKMKKIPVILDVQPVFLRTDLHWIEEK
                     VGSYRAKNTYMWKTLMRNGLMLTGSSDCPVESYDPLLGIYSAVTRCDFTGFPKNGYNP
                     DERIDIYDAVRMYTHNPHYATGQQNINGLIKEGFFADFVVLDRNIFEIDEMDLLDVNV
                     LSTYIAGEKVF"
BASE COUNT         1106 a          716 c          838 g         1048 t
ORIGIN      
        1 atatatactt ttccaggaaa catataacaa ggaaagatat ttggaacttc acccttcggg
       61 gccaaaaaga gattatgatt atcatactga agctatggac agagccaatg aaggcgggat
      121 agatgatgtg gggtgcggag tccttttcgg gcttgataat tacaaatatg attttgtggg
      181 aattctgatg cacgcaaaac atctggaaga tacatgggga tgcgggcccc ataccataag
      241 cgttccgagg atacgccctg ctgatgatat cgatgttagc gattttcaaa acggtgtacc
      301 ggatgatatt ttcaaaagga tcgttgctgt acttagaata gcggtgcctt ataccggaat
      361 gattatgtcc accagagaaa gtgaaaaaac acgaagagag tgtctggaga taggaataac
      421 tcaggtcagc ggaggaagtc gtaccagcgt gggcggatat gaagaagaag aaagagatga
      481 aagtactgca caatttgatg taagtgatac gaggaccctt gatcaggtag tctcctggct
      541 tatagaactg gggtatgtgc ccagtttttg taccgcatgt taccgggaag gacggacggg
      601 agacagattc atgagtcttt tgaaatcagg acagatcgcc aactgctgtc agccaaatgc
      661 tcttatgaca cttaaagaat acctggtgga ttacgcatct ccagagacat acgaaaaggg
      721 agaagctatt attcaaaaaa ggctggccat ggttcccaat gaaaaggtca gagaaatagc
      781 aaaaaaacat ctcaaggaaa tcgaaaacgg aaagagagat tttaggttct gatgaatagc
      841 ctaaatgaaa caccaagagc cggaaggctt cacataagta tattcggcaa gagaaacagc
      901 ggcaaatctt ctttgataaa tgctcttacc gggcaggata tcgccatagt ttccgagcat
      961 gccggcacca ctacggatcc tgtttataaa tccatggaac ttcatcctgt gggacctgtg
     1021 gtttttattg atacggcagg atttgatgat ttcgggaaac tgggagaact gaggcttagc
     1081 cgtacaaagg aagttatcaa aaaaaccgat atagcattgc ttttattcga tgacggcagt
     1141 cttgaccctg aagagggatg gctatccggt ttgagggatg cggatgttcc ggtcattcca
     1201 ataataaata aaacagacct gttggaccgg gcggctttgc agcaaaagat catcatggta
     1261 ttcgggctta ccccgataat ggtaagtgcc gccgatggaa cggggataga ggatatcaag
     1321 aaagccatag ataagctggc gccggaagat ttcaaacggg aaaaaattct gggagacatg
     1381 gtaagcgccg gcgaaacagt tcttctcgtt atgcctcagg acatacaggc accgaaagga
     1441 aggctaatac tgccccaggt acaggtgata agggaacttc ttgataataa atgtactgtt
     1501 gtatctactg tttcggatgg ttttttgaaa gctctggatt cgctttcgag ggcgccttcg
     1561 cttattatta ccgattccca gtgtttcgga tatgtgaata aaaacaagcc cggggagtct
     1621 gcgctgacat ctttttcaat attgctggcg gcatttaagg gagatcttaa aacttttgtt
     1681 gagggagctg cagcgataga ttctctgaca gagaaatcaa gggtgctcat agcggaagcc
     1741 tgtacccatg cacctctcga agaagatata ggaagagagc aaatacccgg gatgctcagg
     1801 gagcgtgcag gcgaaggaat gagagtggac attgttgccg gctcggattt tccaaaggat
     1861 ttgaaaaaat atgatttgat aatccactgc ggctcatgta tgttcaaccg caagcacatg
     1921 atgtcaaggg tagaacaggc aaagacccaa gggatcccta tcaccaatta cggaatagtg
     1981 atggcaaaac tgaaagggat actcgatgat ataactctgc cgcccggccg ctgacccgcc
     2041 cggtcacccg acttggctgc cctgcctcct atttgtaaaa ttactggagt tttaagcaaa
     2101 agggaaggga aaatatagca aaatcaaaat tttaaccctg gaaccggggt tttggcacga
     2161 aaagggttaa aatttcaaat tatatacttt tatcgccgtg ttaagtggtg ttacctgaat
     2221 tcatgcatag cgcaacgaaa agcaatagta tagatgccgg agtaaagcaa aaaagagaga
     2281 taaatgtaag atatttagtc aaaagaaaga ataagtaaaa ttaaaaaact ttttccccgg
     2341 ctatatatgt agagagaaca ttgacatcaa gcaaatccat ttcatctatt tcaaagatat
     2401 ttctgtcaag cacaacaaaa tcggcaaaaa acccttcttt aataagaccg ttgatatttt
     2461 gttgaccggt agcataatga ggattgtgag tatacattct cactgcatca tatatgtcta
     2521 ttctttcatc gggattataa ccatttttgg gaaagccggt aaaatcacat cttgtaacag
     2581 cagagtatat tcccaataaa gggtcatagc tttccacggg gcaatcagaa cttcccgtca
     2641 gcataagacc gttcctcata agggttttcc acatatatgt attttttgct ctatagcttc
     2701 ccactttttc ttctatccag tgaaggtcgg ttcttaaaaa aacgggctgt acatcaagga
     2761 tcacaggtat ttttttcatt ttttcaatca aatccggtgg tgtcatttgt gcgtggatta
     2821 ttctaaaggg caaagctggt tttgttttgg aagacaagat tgtcttttca attgcattta
     2881 tggtgatttc cagagccctg tcaccgatag catggattgc aggttgaaga cctttgtcat
     2941 aggcagtttt catcttatta taaagttcat cttctgtgca tatcatcaca ccggaatcac
     3001 cactgttttt ataaggatcc cttaaggctg ctgattctgc gccaagtgac ccatcggtga
     3061 acaacttata agagcctaaa tggacttttg cattagggtc attgttgatt ttagttgtag
     3121 catcaaataa ttcatcaagg caaacataga ctctgacagg tagtaagcca atgttttcaa
     3181 ggtgtttata agtttctata gattctttgt agttccatat cttggcagaa taagtatgta
     3241 ttcccgtaat ccctctgcta ctcatgtctt tgaagacttt catcattatt ttttctctta
     3301 aggcgatatc agaaagagga tctttgatta tgttatcgat aacctgtgtg cattgctctc
     3361 tcaatatgcc gttggggttt ccgtctgctt ctgtttcgac tataccgccg ccttcgggat
     3421 tgtaaccctt tattactccc gctaatgtca gaccctctgt gtttacaaca attgcatgta
     3481 gacaaactct ccttatgact atagggtgtt tattagacac attatccaaa tcatgccttg
     3541 taggcatccg tttttctttg aataatgtgt catcaaaatt gacccctttt atccagcatc
     3601 cctccggtat ttcctttgat ttgtttttca tgactgatat catttcttta atgcttctga
     3661 tatttgaaag atccacagcg actaaattct gacagaacgc gtacatat
//
LOCUS       DAONHK010000107         3631 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_20416_length_3631_cov_4.407159, whole genome shotgun sequence.
ACCESSION   DAONHK010000107 DAONHK010000000
VERSION     DAONHK010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3631
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20416_length_3631_cov_4.407159"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="PLM92_04605"
     CDS             complement(<1..286)
                     /locus_tag="PLM92_04605"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HPR24449.1"
                     /translation="MEFNNCSLIILAGGKSSRMGQNKAHLRWNAKCFLDIIIEKGKSL
                     GFGEIVLSGYRGKVSDCFSVEDELKERGPLGGLHACFKAASFEDCFVITVD"
     gene            complement(321..1136)
                     /locus_tag="PLM92_04610"
     CDS             complement(321..1136)
                     /locus_tag="PLM92_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004838145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HPR24450.1"
                     /translation="MAPLKPAEILDATINIGVGKANSSAAKLIMLGILAGAFIAFAAA
                     GSNMAAFNALGDAGTFGIGRWIAGMVFATGLMLVVCCGAELFTGNTLMVCSVLEKKIN
                     VSGMLRNWVIVYIANMVGSVLIAYLVVNSGLLASGDGALGATTVSIAAGKCNLEFLPA
                     FYRGILCNWLVCLAVWMAFGSDTMVGKFFSCFFPISLFVTSGYEHSVANMYYIPAGLF
                     AKGEFGASLDPAVIENLTWGGFFIDNLIPVTLGNIVGGMILVGMIYWFSYKKA"
     gene            1510..2688
                     /locus_tag="PLM92_04615"
     CDS             1510..2688
                     /locus_tag="PLM92_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HPR24451.1"
                     /translation="MDTFQKKMDIIYFDNSATSFPKAPGLGKAIGEHIDNNGFNICRG
                     SYEKSYQLQSTIIETREQMCSMFGFDLPQNVIFCSGATFGLNLIIKGILETGDHIITS
                     SMEHNSVARPVTVMTSKGIIWDIAKCRRDGSLDPSAVEALICKKTKLVLLAHASNVSG
                     SMMPIDEIGRLCKKHGVFFALDAAQTAGSKTIHMKNSNIDALALPGHKGLLGPQGIGA
                     VLLSERFASELGTVIEGGTGSLSDNFEMPSFMPDKFETGTLNIPGIIGLAHSLKYIKN
                     LGISEINAKKKILTEKFLSGILDIPHVNLSGIRSMDMRCSVISLDFKYIDNSNAAYLL
                     EKDHGIITRCGLHCSPMSHKTLGTYPQGTVRFSIGFFNTEKEVDTAISAINEIGKKHQ
                     "
     gene            complement(2682..2975)
                     /locus_tag="PLM92_04620"
     CDS             complement(2682..2975)
                     /locus_tag="PLM92_04620"
                     /inference="COORDINATES: protein motif:HMM:NF023251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HPR24452.1"
                     /translation="MEEKTAGIKNGDETDKASERKKAIWMVVTFENTFKASKASKILE
                     EEGFEGGLIPVPREIKASCGTAWRGGKNDKDKVADTLNKNKIGYENIYEVAFY"
     gene            complement(2993..3598)
                     /gene="yedF"
                     /locus_tag="PLM92_04625"
     CDS             complement(2993..3598)
                     /gene="yedF"
                     /locus_tag="PLM92_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008977018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HPR24453.1"
                     /translation="MRIDVTGEVCPVPVIKAKKALKEMSSGTLEVLTDSDVAVKNLEK
                     MARTENCLFSFKEEGDIFHVFIEKEEKENVAQGEEDKKVLEAQTLKGSKVVAIASDRM
                     GSGDAELGKILIKGFIFALTQMDELPASVLFYNSGAHLTVEGSPCIDHIKALEAAGVQ
                     IFTCGTCLDHYGITDKLKVGSVTNMYETVEKMLKAQSVIRP"
BASE COUNT          990 a          886 c          686 g         1069 t
ORIGIN      
        1 aatccacagt tattacaaaa cagtcttcaa aagaagcggc tttgaaacat gcatgcagcc
       61 cgccgagagg gcccctttct ttcaattcat cttctacaga aaaacaatcg ctcacttttc
      121 ccctgtatcc ggacagtact atctcaccga aaccgagact ctttcccttt tcgatgatta
      181 tatccagaaa acattttgcg ttccagcgca gatgtgcttt gttttgcccc atcctggaac
      241 ttttgcctcc ggcaaggata ataagtgagc aattgttaaa ttccataata attccggttt
      301 tgcgcccgga catacgcttt ttatgctttc ttatatgaga accagtatat cataccaacc
      361 agtatcattc cacctacaat gtttcctaaa gttacaggta tgagattgtc gatgaaaaat
      421 cctccccatg tcaggttttc tataacggcc ggatcaaggg aagcgccgaa ttcgcctttc
      481 gcaaaaagac ctgcgggtat gtagtacatg ttggcaacac tgtgttcata tcccgaggta
      541 acgaacagtg aaatggggaa gaagcaactg aagaatttcc ccaccatggt gtctgagccg
      601 aatgccatcc atactgccag acataccagc cagttacaaa gtattcccct gtaaaatgcc
      661 gggaggaatt ccagattaca cttacctgcc gctatactta ccgttgttgc tccaagagct
      721 ccgtcaccgg atgcaagcag tcctgagttc actaccaaat aggcaataag tacagatccc
      781 accatgttgg ctatatagac tatcacccag tttctcagca tgccgctgac attgatcttc
      841 ttttccagaa ctgaacatac cataagtgtg tttccggtaa aaagttccgc tccgcagcaa
      901 acgaccagca tcagtccggt ggcgaatacc attcctgcta tccatctgcc gataccaaat
      961 gttcccgcat ctcccagggc attgaatgcc gccatattcg agcctgcagc cgcaaaagca
     1021 ataaaagcgc ctgctaaaat tcccagcatg atcagctttg cagcgctgct gtttgctttg
     1081 cccacaccga tattgatagt tgcatccagt atttctgccg gttttaatgg tgccatgctg
     1141 ttactgggct ttggtgctgt gaccttaggt gcggcctcct gtttttccat tggttctaat
     1201 ggtgccattt ttgaattttc gtacgagagc tcaacccaaa aataaacggt atcaaaccgc
     1261 tcgtacactc cttccttttt ttttaataac ttctaacatc ggatatcgct atccgaaaaa
     1321 tacatcatca tagacagtat atttagtacg atataaattg tcaatacgtt tttcaaaagg
     1381 atacacacat cacttttata tatgaagcat aagtatatag ctgcatcatt tctattcata
     1441 ttatctattt gaattcatac acttcaatat tctataatga taccggaggc tgaaaggcaa
     1501 tgttcccaaa tggacacctt tcagaaaaaa atggacataa tatactttga taacagtgcg
     1561 acttcgttcc caaaggctcc cggtctcgga aaggccatag gcgaacatat agataataac
     1621 ggcttcaata tttgccgtgg cagttatgaa aagtcttatc agcttcaaag caccataatt
     1681 gaaacaaggg aacaaatgtg ttctatgttt ggttttgacc tgccgcaaaa tgttattttc
     1741 tgctccggag caacttttgg actgaatttg atcataaaag gtattcttga aacaggggat
     1801 cacattatca catcttccat ggaacacaat tccgtggcca ggcctgtaac agttatgaca
     1861 agcaaaggta tcatatggga tatcgccaag tgccgccggg acggaagcct ggatccgtcg
     1921 gcagttgaag ctttgatctg caaaaagaca aaactcgtgc ttcttgccca cgcatccaat
     1981 gtcagcggca gtatgatgcc aatagatgag atcggtcgtc tatgcaaaaa acacggcgtt
     2041 ttttttgctt tggatgccgc tcagaccgca ggcagtaaaa cgatccacat gaaaaacagt
     2101 aatatcgatg ccctggcatt gccgggtcat aaagggcttc tcggtcccca gggaattggg
     2161 gcggtactcc tttccgaaag attcgcctct gaacttggta ctgtcataga aggcggtacc
     2221 ggcagtcttt ccgataattt tgaaatgcct tccttcatgc cggacaaatt tgagaccggt
     2281 acccttaata tccccggaat aatcggcctc gcccattccc tgaaatatat caaaaacctg
     2341 ggcataagtg agataaatgc aaaaaaaaag atccttacgg agaagttttt aagcgggata
     2401 cttgatatcc cccatgtcaa cctttcgggg atcagaagca tggatatgcg ctgttccgtt
     2461 atctcgttag attttaagta cattgataac agcaacgcag cttatttgct ggaaaaggac
     2521 catggtatca taacaagatg cggcctgcat tgttcaccca tgagccataa gaccctcgga
     2581 acatatccac aaggcacagt acgtttttcg ataggttttt tcaacactga aaaagaagta
     2641 gataccgcca tatcagccat aaatgaaatc ggcaaaaaac atcagtaaaa agccacttca
     2701 taaatgttct catatcctat tttgttttta ttcagggtgt ctgcaacttt atctttgtca
     2761 tttttgccgc cacgccaggc agttccacag cttgctttta tctctctggg aacaggtatc
     2821 aggccgccct caaaaccctc ttcttcaagt atttttgacg cttttgaggc tttgaacgtg
     2881 ttttcgaaag tgaccaccat ccagatcgct ttttttcttt ctgaagcttt atcggtttca
     2941 tccccatttt tgattccggc tgttttttct tccatttctt cccatcccac gcttacggtc
     3001 ttatcacgct ttgtgccttt aacattttct caactgtctc atacatgttg gtgaccgatc
     3061 ccaccttcaa tttgtcagtt atgccgtaat gatcaaggca tgtgccacag gtgaatatct
     3121 gtactccggc cgcctcaaga gctttgatat ggtctatgca gggagatcct tcaacggtaa
     3181 gatgtgcccc gctgttataa aaaagcaccg atgccggcag ttcatccatt tgggtgagag
     3241 caaaaataaa gccttttata aggatcttgc ccagttcagc atcgccgctc cccatcctgt
     3301 ctgaggcgat ggctactact tttgatccct tcaaagtctg tgcttccaag acctttttat
     3361 cttcttctcc ttgcgcaaca ttttcttttt cttctttttc tataaaaaca tgaaaaatat
     3421 caccttcttc tttgaatgaa aataagcagt tttctgttct tgccattttc tcaaggtttt
     3481 taactgccac atcactgtcc gtcagcacct ccaaagtacc tgaagacatt tctttcaatg
     3541 ccttcttggc tttgataacg ggtaccggac aaacttcccc tgtaacatct attctcatat
     3601 tgatcctctc atttctcaaa taattaccaa t
//
LOCUS       DAONHK010000108         3617 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_20530_length_3617_cov_4.094891, whole genome shotgun sequence.
ACCESSION   DAONHK010000108 DAONHK010000000
VERSION     DAONHK010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3617
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20530_length_3617_cov_4.094891"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1150
                     /locus_tag="PLM92_04630"
     CDS             <1..1150
                     /locus_tag="PLM92_04630"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HPR24454.1"
                     /translation="ALWIYVVGEVNPDTTKKYDGIKIDFTNEESLAQNGLALLDPGVL
                     TVDVTVEGKRSAVNGVKEENIEVMADLTRRQKGKNSIHLEVNLPGEVKLKSISAENID
                     VTIENRISEEKPVKVSFKGKLEDNREPGEAEVNPDRVIVSGAESAVRSVSHVKAEVNV
                     KDFKNDKLSVETKALPVNRKGVAVRYLELSSDSIQVKARLMQTKEVSLKVNVKGEIKE
                     PYQLDSIDIPETVVIKGSEEDIENISVIQAEDVYIGDITKTSYIPLDFIMPSGVETAS
                     ESQDMSIKVTIKALSSKSFDYKSEDVDAKGLKTGLSASFEKAGIKVTARGMEDIVSGL
                     SGGDFGISVNLSELDAGTHKVKLKVTYTKKLSSVSVSPKEILVTISEE"
     gene            1164..2516
                     /gene="glmM"
                     /locus_tag="PLM92_04635"
     CDS             1164..2516
                     /gene="glmM"
                     /locus_tag="PLM92_04635"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009644490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HPR24455.1"
                     /translation="MSRLFGTDGVRGIANSELTPELAFNLGKAGAHVLNKEKRKPVVL
                     IGRDTRISGDMLESALCAGILAVGGNVIKVGILPTPAVAYLVRYFDADAGVMISASHN
                     PFEYNGIKFFNEKGFKLDDDIEDAIEDIIVRDIDVNSHITGDRLGKCIEGEEDALELY
                     AAFLKSTIDRDLKGIKIVLDCANGAAYKVAEKVYKDLGADVTVIGNRPNGLNINEGCG
                     STHPGQLQKKVIEEKALMGLAFDGDADRLIAVDEKGNIIDGDRQICICAKMMKEEGTL
                     KDNKVTATVMSNIGFHKYMEDLGCAVNTTKVGDRYVLEEMVKTGCVIGGEQSGHIIFL
                     DHTTTGDGILSSLQLIKAVKDSGLEPSELFGEVAIYPQVLRNAKVRNENKNKYEKDPD
                     IVKEIERLQKEMDGEGRILIRPSGTEPLVRVMIEGRNEGYITDLAEDLAELITRKLGQ
                     "
     gene            2589..>3617
                     /gene="glmS"
                     /locus_tag="PLM92_04640"
     CDS             2589..>3617
                     /gene="glmS"
                     /locus_tag="PLM92_04640"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005999772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HPR24456.1"
                     /translation="MCGIVGYIGGGKAKEIIIDGLKRLEYRGYDSAGIALCIDGKIET
                     RKHVGGIENLEKIIDDKEYESNIGIGHTRWATHGAPSDINAHPHNNEDNTVTVVHNGI
                     IENYTELKESLIKDHGIKFRSETDSEVIAQLIGALYDGDIHEAVNNAVKKMRGAYAIG
                     VVSAHEPDKIIAVRKDAPLVAGIGKGCNFIASDIPALLKYVREIFLIENNETIVVTKN
                     EVKIYDEKGQTVKRDVFHVTWDADAAEKSGYPHFMLKEIHEQPKGLEDTLLRRLDKDG
                     RIKLDGITMTKEDIESFNKIYIVACGTAYHAGLVGKYTIEKMARIPVEVDIASEFRYR
                     DSFVDENTL"
BASE COUNT         1284 a          608 c          928 g          797 t
ORIGIN      
        1 tgctctttgg atatatgtag tgggggaggt aaatcccgac acaacaaaaa aatatgatgg
       61 cataaagatc gattttacaa atgaagaaag tctcgcccaa aatggactgg cgcttttgga
      121 tcccggcgta ttaactgttg atgtaacggt ggagggcaaa aggtcggctg taaacggagt
      181 aaaagaagag aatatagaag tcatggcaga tcttacaagg agacagaagg gcaaaaacag
      241 tatccatctt gaagtaaatc ttcccggaga ggtgaaactt aagagtatat cagcagaaaa
      301 tatcgatgtg accatagaaa acaggatatc ggaagaaaaa cctgtaaaag tatcttttaa
      361 gggaaaactt gaggataata gagaaccggg agaagctgag gtcaatccgg acagagtaat
      421 agtcagcggg gctgaatctg cggtcagatc tgtaagtcat gtaaaggccg aggtgaacgt
      481 aaaggatttc aagaacgaca aactttctgt ggaaactaaa gcattgccgg taaaccgtaa
      541 aggtgttgct gtaaggtatc tggaactgtc ctctgactcc atacaggtca aagcccggct
      601 gatgcagaca aaagaagtct ccctcaaggt caatgtgaaa ggagaaataa aggaaccgta
      661 tcagctggac agcatagata tacctgagac agtggtcata aaggggtcag aagaggatat
      721 tgaaaacatt tctgtgatac aggcagaaga tgtatatata ggagatataa caaaaaccag
      781 ttatatcccc ttggatttta taatgccatc cggcgtggaa acggcctcgg agtcgcagga
      841 tatgagtatt aaagtgacca tcaaagcgct ttcatcaaag tcttttgatt acaaaagtga
      901 agatgtagat gcaaagggtc taaagacagg gctttcagct tcattcgaaa aggccggcat
      961 aaaagtaaca gccaggggca tggaagatat tgtttcgggt ctttccggag gggactttgg
     1021 gatatcggta aatctttcag aacttgatgc gggaacacat aaagtcaaac tgaaagtaac
     1081 ctatacaaag aaattgagca gtgtatctgt ctctccaaaa gagatactgg taaccataag
     1141 cgaagaataa aggaaaacat aatatgagca gattattcgg tacagacgga gtaagaggaa
     1201 tagccaattc ggaactgaca cctgaactgg cttttaatct gggcaaagcg ggagcccatg
     1261 tactaaacaa agaaaaaagg aagccggtgg tccttatcgg tagggatacc aggatatcgg
     1321 gagatatgct ggaaagcgcc ctttgtgccg gtatactggc tgtgggcggc aatgttataa
     1381 aagtgggtat actgccaaca ccggcggtgg cataccttgt caggtatttt gatgcggatg
     1441 caggggtaat gatatccgct tcccataatc cttttgagta taacggcatc aagtttttca
     1501 atgaaaaagg attcaaactt gatgatgata tcgaagatgc cattgaagat ataatagtaa
     1561 gagatataga tgtaaacagc cacataacag gagacaggct gggcaaatgt attgaaggtg
     1621 aagaggatgc gctggagctt tatgctgctt ttctcaaaag caccatagac agagatctaa
     1681 aaggcataaa gatagttctt gactgcgcca atggtgccgc atataaggtg gctgaaaaag
     1741 tctacaaaga tctgggagcc gatgtaacgg taataggcaa caggcccaac ggactaaata
     1801 taaatgaagg ttgcgggtcg actcatcccg gtcaactgca gaagaaagtg atagaagaaa
     1861 aggcactcat ggggctggca ttcgatggag atgcggacag gctcatagcc gtcgatgaaa
     1921 aaggaaatat aatcgacggt gataggcaga tctgcatttg cgcaaaaatg atgaaggaag
     1981 aaggcacact taaggacaac aaagtaactg ccaccgtaat gagcaatatt ggatttcaca
     2041 agtatatgga agacctggga tgtgctgtga acaccacaaa agttggagac aggtatgtgc
     2101 ttgaagaaat ggttaagaca ggatgtgtta ttggaggaga acagtcggga catataattt
     2161 ttctggatca cacaactacg ggagacggca tattatcatc acttcagctg ataaaagccg
     2221 taaaggattc cggccttgaa ccttcagaac tcttcggcga agtggccata tatccccagg
     2281 tgctgagaaa tgcaaaagtg agaaatgaaa acaaaaataa atacgaaaaa gatccggata
     2341 tagtaaaaga gatagaaagg ctccagaaag aaatggatgg agaagggcgt atacttataa
     2401 ggccttcggg caccgaacct ctggtgaggg tcatgatcga aggcaggaat gaaggataca
     2461 taacagacct tgcagaagat cttgctgaac tgataacaag aaaactggga caataaaacc
     2521 gggacgacag tattttcggt gaaaaagtcc tgagaaaggc aaaataaatt ttcgggagga
     2581 aaagaaatat gtgcgggatc gttggttata taggtggcgg taaagcaaaa gagataataa
     2641 tagacggact aaaaagactt gagtacagag gttatgattc ggcgggcatc gccctgtgta
     2701 tagacggcaa aatcgaaaca agaaaacatg ttgggggaat cgaaaacctc gaaaaaataa
     2761 tagatgacaa agaatatgaa agcaacatag gtatcggaca tacaagatgg gcaacccacg
     2821 gagcaccttc tgacataaac gctcatccgc ataacaatga ggataatacg gtcacagtcg
     2881 tccacaacgg aataatagaa aactataccg aactaaaaga atcccttatc aaagaccatg
     2941 gtataaaatt tcgttcagaa acggattcgg aggtaatagc acagcttata ggagcgcttt
     3001 atgatggaga tattcatgaa gctgtaaata atgctgtaaa aaaaatgagg ggagcctatg
     3061 ctataggtgt ggtatctgcc catgaaccgg acaagataat agcagtgaga aaagatgcgc
     3121 ccctggtggc gggaatagga aaaggctgta acttcatagc ctcggatata cctgctcttt
     3181 taaaatatgt gcgtgaaata tttttgatag aaaacaacga aaccattgta gtcacaaaaa
     3241 atgaagtaaa aatatatgac gaaaaaggtc aaacggtaaa gagagacgtt ttccatgtga
     3301 catgggatgc tgatgcggcg gaaaaaagcg gatatcctca ttttatgctg aaggaaatcc
     3361 acgaacagcc caagggactg gaggataccc tgctgaggag actcgataaa gacggaagaa
     3421 tcaaacttga cggtataacc atgacaaagg aagatataga aagttttaat aaaatataca
     3481 tagtggcctg cgggactgca tatcatgcag ggctggtggg gaagtataca atagagaaaa
     3541 tggccaggat acccgttgaa gtcgatatag cttcggagtt ccgttatcgg gattcctttg
     3601 ttgatgaaaa cactttg
//
LOCUS       DAONHK010000109         3605 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_20628_length_3605_cov_4.605070, whole genome shotgun sequence.
ACCESSION   DAONHK010000109 DAONHK010000000
VERSION     DAONHK010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3605
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20628_length_3605_cov_4.605070"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..769
                     /locus_tag="PLM92_04645"
     CDS             <1..769
                     /locus_tag="PLM92_04645"
                     /inference="COORDINATES: protein motif:HMM:NF019555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPR24457.1"
                     /translation="ERKFKSTNRLLYDEKNEINVNGRERFPKYKGAIGIKTGQTGTAG
                     YCLAGCAKRKGTEYIAVILFAPDDLSRFSDCIELLDFGFDNYHTYRAYNPGEPAGRVK
                     IKNGSKLKVDAEVKDGAYATLSNEGSDSLVSEKIVMRDDLKAPVKKGTRAGTVKLYVD
                     DIKVGEAELVVSESVKKGGPWTAIGISDLAAFIILILLIILFIALLIILIRRANRKKA
                     EKARKRREEEAKKRKEDEAKRRMAEEAENRRRREWPY"
     gene            780..2645
                     /gene="uvrC"
                     /locus_tag="PLM92_04650"
     CDS             780..2645
                     /gene="uvrC"
                     /locus_tag="PLM92_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005999812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HPR24458.1"
                     /translation="MFDIEENLKKLPDKPGVYIHKDRLGNVIYVGKAISLKKRVRQYF
                     RAYRNTDPKVRAMVSYISEFEYITTGTEMEALILECNLIKKYMPRYNVLLRDDKTYPF
                     IKVTTTEEFPRIMKTRKIDRKGDKYFGPYTDAGAVAQMVELLNTIYVLKRCPTSKFAK
                     GSRPCLNYHIGQCQGICKGGVDREEYMQRIGSAMEFLKGRNRKISDYLKEKMESASER
                     MDYEEAARYRDYLLAAKTLSEKQRVVLKNTKDIDVVHWAGEGHVVLFYVRDGKLTGRE
                     TYTLEVNPEDEEKDVITAFLKQHYGKQAAGPREILVSLMPEEAQMIEEYLSGLWPRKV
                     TLLVPVKGEKKALMDLVKKDIEEIRKTVDDREENRRERDAAIGREVAEFLEKTGIHKK
                     RGEERAYRIEAYDISTTGGIETVGAMVVFEGARPDKKAYRRFRIRTAEDSDDYGGIRE
                     VLYRRFKRGLAGEPGFDEMPDIVLIDGGRGHLSAALSVTEAMKIDIHVAGMVKDEKHR
                     TRGLVYKKKEAAKAGDEEISEYDLLSNSLLYRYIGTIQEEVHRFAITYHRGLRDKKSI
                     KSVLDGIKGIGPAKRNALLSHFGSIENIKKAKEQELLEVQGITPSLAQNIREYFN"
     gene            2695..2976
                     /locus_tag="PLM92_04655"
     CDS             2695..2976
                     /locus_tag="PLM92_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005999814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HPR24459.1"
                     /translation="MTDEQKKDQITEEESEVITLEFDDGTEVECEIMGVFDAGGKDYI
                     ALIPDDKTDDVYIYGYKETGEDEFELIDIEDDEEFQKAVAEFDKLVEEE"
     gene            complement(2985..3146)
                     /locus_tag="PLM92_04660"
     CDS             complement(2985..3146)
                     /locus_tag="PLM92_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPR24460.1"
                     /translation="MEKKNLIAGIAFLVGTAICVFSLFTREFQTYYVCGAVVFTVVGV
                     AFLKRANTR"
     gene            3226..>3605
                     /locus_tag="PLM92_04665"
     CDS             3226..>3605
                     /locus_tag="PLM92_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003495600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPR24461.1"
                     /translation="MKNYDIIVAGAGASGVYLSYELAKIKNRATVLMIDKGAPLEERI
                     CPIKKGVVSSCIKCEPCHIMNGYGGAGTLSDGKYNLTTHFGGDLHRYIGTENAMDLMN
                     YVDEVLCKMGGADAKLYSTANSDL"
BASE COUNT         1264 a          655 c          923 g          763 t
ORIGIN      
        1 agaaagaaaa ttcaaaagta ccaataggct gctttatgat gaaaaaaatg aaataaatgt
       61 taacggacgg gaacgtttcc caaagtacaa aggtgccatc ggcatcaaaa ctgggcagac
      121 gggcacggcg ggatactgtc ttgccggatg cgccaaacgc aaagggacgg aatatatcgc
      181 agtgatactt tttgcccccg acgatctttc cagattttcc gactgcatag aactgctgga
      241 tttcggattt gataattacc atacctacag ggcatataac ccgggagagc cggcaggcag
      301 ggtaaaaatc aaaaacggca gcaaactgaa agtggatgca gaagtgaaag acggagctta
      361 tgccacgctt tccaatgagg ggtcggattc tctcgtttcc gaaaagatcg taatgagaga
      421 tgatttgaaa gcacctgtca aaaaaggcac aagggcagga acggtcaagc tttatgtgga
      481 tgacataaaa gtcggggaag cggaactggt ggtttcggag tctgtcaaaa aaggcggtcc
      541 ctggacggcc atcggtattt ctgatctggc ggcatttata atacttatcc tgctcataat
      601 tcttttcata gcattgctta ttatactgat acgccgggca aacagaaaaa aggcagaaaa
      661 agcaagaaag agaagagaag aagaagcgaa gaaaagaaaa gaagacgaag caaaaagacg
      721 aatggcagaa gaggcagaaa acagaagacg cagagaatgg ccctattaaa ggcgtaaaaa
      781 tgtttgatat cgaagaaaac ttaaaaaaac tcccggacaa accgggagtt tatattcata
      841 aggacagact tggcaacgtt atatatgtgg gaaaggctat ttccctgaaa aaacgtgtca
      901 ggcagtattt tcgtgcatac agaaacacgg acccgaaggt cagagccatg gtttcctaca
      961 tttcggaatt tgaatatatc accacgggca cagagatgga agcgcttata cttgaatgca
     1021 atctgatcaa aaagtatatg cccagatata acgtcctgct ccgcgacgac aaaacatatc
     1081 ccttcataaa agtgaccacc acagaggaat ttcccaggat aatgaagacc cgcaaaatag
     1141 acaggaaagg cgacaaatat ttcggacctt ataccgatgc gggagcagtg gctcagatgg
     1201 tggaactttt gaacactata tatgtattaa aaagatgtcc aacatcaaag tttgccaaag
     1261 gatcaagacc gtgcctgaat tatcatatcg ggcagtgtca gggcatatgc aaaggcggtg
     1321 tggacaggga agaatacatg caaagaatag gttcggcaat ggagtttcta aagggcagaa
     1381 acagaaagat atccgattac ctcaaagaaa aaatggaatc cgcatcggaa agaatggatt
     1441 atgaagaggc tgccagatac agagactatc ttcttgcagc aaagaccctg agcgagaaac
     1501 agagggtggt actgaaaaat acaaaggata ttgatgtggt ccattgggcc ggcgaaggac
     1561 atgtcgtgct tttttatgtg agggatggaa agctgacggg aagagaaaca tatactcttg
     1621 aagtaaatcc tgaggacgaa gagaaagatg ttattacggc atttctgaaa cagcattacg
     1681 gcaaacaggc ggcgggtccc agagaaatac tggtctcatt gatgccggaa gaggcacaaa
     1741 tgatagagga gtacttaagc ggtttatggc caagaaaggt aaccttgctg gtgcccgtta
     1801 aaggcgaaaa aaaggctctg atggatcttg tgaaaaaaga tatcgaagag ataagaaaga
     1861 ccgtggatga cagggaagaa aaccgcagag aaagagatgc cgccatagga agagaggtgg
     1921 ctgagttcct ggagaaaacg ggcatccata aaaagcgcgg ggaggaaaga gcatacagaa
     1981 tagaggccta tgatatttcc accaccggcg gcatagaaac agtgggggca atggtggtct
     2041 tcgaaggggc aagaccggac aaaaaagcat acaggagatt cagaataaga acggcggaag
     2101 attcggatga ttacggtggc ataagggaag tgctttacag gcgattcaaa agaggtctgg
     2161 ccggtgagcc cggatttgat gaaatgccgg atatagttct gatagacgga ggcagaggcc
     2221 atttgagcgc tgctttatcg gtgactgaag ccatgaaaat agacatacat gtggccggca
     2281 tggtaaagga tgaaaaacac aggaccagag gactggtata caagaaaaaa gaagcagcaa
     2341 aagcaggaga cgaagagatc tcggaatatg accttttgtc aaactcactg ctttacagat
     2401 atatagggac gatccaggaa gaagtccaca gatttgcaat aacctatcac agaggattga
     2461 gagacaaaaa aagcatcaag tctgtgctgg acggcataaa aggcatcggt ccggcgaaac
     2521 gcaacgcact gctttctcac ttcggaagca ttgaaaacat aaagaaagcc aaagagcagg
     2581 aacttcttga agtgcagggt ataacgcctt ctctggcaca gaacataaga gaatatttta
     2641 attgataaac acatgtttag atgatatagt ttaaccgtaa aggaggaaga cccaatgaca
     2701 gatgaacaaa agaaagatca gatcacagaa gaagaatcag aagtaataac tcttgaattt
     2761 gatgacggaa cagaagttga atgcgagatc atgggcgttt ttgatgcagg agggaaagac
     2821 tatatagccc ttatcccgga cgacaaaacc gatgatgtct acatctacgg atacaaggaa
     2881 accggcgaag atgaatttga actcatcgat atagaagatg atgaagaatt ccagaaagcc
     2941 gttgccgagt tcgacaagct cgtggaagaa gagtaattga tatttcagcg ggtattggct
     3001 cttttgagaa atgccacacc tacaacggta aatacaacag caccgcagac atagtatgtc
     3061 tgaaattctc ttgtaaaaag gctgaataca catatggctg tgcccacaag aaaagcgatg
     3121 cccgcgatca gatttttttt ctccatgata atgacctccg ggaaatatta taacataagg
     3181 gtcatttgca tggaagattt gtttttgtaa ggaaagaatc aaaaaatgaa aaactatgat
     3241 attatagttg ccggtgccgg cgcaagcggt gtttatcttt cttatgaact ggccaaaata
     3301 aaaaacagag caacagtact tatgatagac aaaggagcac cccttgagga gaggatatgt
     3361 ccgatcaaaa agggtgttgt ttcttcatgt ataaaatgtg aaccctgcca tataatgaac
     3421 ggatacggag gagcaggtac gctttccgac gggaaatata acctgacgac ccatttcgga
     3481 ggagatctgc acagatatat cggaacagaa aatgccatgg acctcatgaa ctatgttgat
     3541 gaagtgcttt gcaaaatggg cggtgcggat gccaaacttt attccactgc aaattcggat
     3601 ctgaa
//
LOCUS       DAONHK010000110         3590 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_20735_length_3590_cov_3.306648, whole genome shotgun sequence.
ACCESSION   DAONHK010000110 DAONHK010000000
VERSION     DAONHK010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3590
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20735_length_3590_cov_3.306648"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="PLM92_04670"
     CDS             <1..199
                     /locus_tag="PLM92_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HPR24462.1"
                     /translation="VDYDVTGSIGKRYRRQDEIGTPFCICTDFDTIDDGCVTIRDRDT
                     MEQVRLPIEEVRKYIEEKLDF"
     gene            213..2843
                     /gene="ppdK"
                     /locus_tag="PLM92_04675"
     CDS             213..2843
                     /gene="ppdK"
                     /locus_tag="PLM92_04675"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006000291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HPR24463.1"
                     /translation="MKKFVYSFNEGSKDMRDLLGGKGANLAEMTKIGLPVPFGFTVTT
                     EACNRYYEEGKKIGDDIIKKIFEKLADLEKVTEKKFGNPENPLLVSVRSGAVFSMPGM
                     MDTILNLGLNDETVEGLAKLTDNERFAYDSYRRFIQMFGDVVMEIPKTKFDNIFEAKK
                     DEKGCSFDVDLKTEDLRSIITDYKKLVKTEIKRDFPQEPKDQLMEAIMAVFRSWNNER
                     AILYRQLNNIPSDLGTAVNVQSMVFGNMGNTSGTGVAFTRSPVNGENRIFGEFLVNAQ
                     GEDVVAGIRTPQPIEEMAEAFPEVYKEFMRISELLEKHYTDMQDMEFTVERNKLFMLQ
                     TRNGKRTAAAAVKIAVDMEQEGLINKKTAVTRLEPAQIDQLLHPKFDEAELKKAEPIT
                     KGLAASPGAAAGRIYFTAEEAVAAVNNGEKAILVRLETSPEDLAGMVVAEGILTARGG
                     MTSHAAVVARGMGKCCVSGCSEIKVEEEAKKMTAGGKVFKEGDFISLDGSEGVVYKGL
                     ISTVSPELSGDFGMIMKWADEVRTLGVRTNADNPRDARQAVDFGAEGIGLCRTEHMFF
                     EEERISNVRRMILADSEEERRSALALLLPYQKGDFKGIYEVMEDRPVTIRLLDPPLHE
                     FLPHTEEEIKELSKQINVPYEKLSKKVEELHEFNPMLGHRGCRLAVTYPEIAEMQAEA
                     IIMAAIELKKEKKMDITPEIMIPLVGLEKELAFVKQTVVDTINRIFEREGVKLDYMVG
                     TMIEIPRAAILADEIAKEAEFFSFGTNDLTQMTFGFSRDDTGNIIKEYRDKLIFEEDP
                     FQTLDISGVGQLVKMAVEKGKQTRPNIKLGICGEHGGDPKTVGFCHEIGLNYVSCSPF
                     RVPIARLAAAQAAVKAEKKS"
     gene            2866..3414
                     /locus_tag="PLM92_04680"
     CDS             2866..3414
                     /locus_tag="PLM92_04680"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HPR24464.1"
                     /translation="MKVLIFNGSPRLNGNTRTALVALKEETEQKMPDSETELIDAYRK
                     NISGCINCDACKHNGGECVQEDDTVEIIEKIYDADILIFGSPVYWWGITAKLKLIIDK
                     MYSKSEQLSKTKKKVGVIAVGMAGLTDPEYKLITDQFQCICDHLNWEMMFSEAFVSEN
                     PDSLKKNKEKLTEIKDLHRKFI"
BASE COUNT         1271 a          618 c          879 g          822 t
ORIGIN      
        1 ggtggattat gatgtgacgg gatccatagg caagagatac agaaggcagg acgaaatcgg
       61 tacaccgttt tgtatatgca ctgattttga tacaatagat gacgggtgcg tgaccataag
      121 agacagagat acaatggagc aggtaaggct gccaattgaa gaagtgagaa aatatatcga
      181 agaaaaactt gatttttaag gagtgtaaaa atatgaagaa atttgtatat tcttttaatg
      241 agggctcaaa ggacatgaga gatctcctgg gcggaaaagg tgcaaatctg gccgaaatga
      301 caaaaatagg tctgcccgta cccttcggtt ttacggttac aactgaggca tgtaacagat
      361 actatgaaga aggcaaaaaa atcggtgacg atataataaa aaaaatattt gagaagctgg
      421 cagacctgga aaaagtaact gaaaagaaat tcggaaatcc ggaaaatccg ctgctggttt
      481 ctgtccgttc gggagcagtt ttttccatgc ctggaatgat ggatactata ctgaatctgg
      541 gcctcaatga tgaaacggtt gaaggcctgg caaaacttac cgataatgaa aggtttgcat
      601 atgacagtta cagaagattc atacagatgt tcggtgatgt ggtcatggaa atccccaaga
      661 caaaattcga taatatattt gaagcaaaaa aagatgaaaa aggatgcagt ttcgatgtgg
      721 atctaaaaac tgaggatctg agatctataa tcactgatta caaaaagctt gtaaaaacag
      781 aaatcaaaag agattttccc caggaaccga aagaccagct gatggaagcc ataatggcgg
      841 ttttccgttc atggaacaat gaaagagcta tactttacag acagctgaac aatatacctt
      901 ctgatctggg gactgcagtg aatgttcagt ccatggtatt cggaaacatg ggaaacacat
      961 caggaacagg tgtagcattc accagaagtc ccgtaaacgg cgaaaacagg atattcggag
     1021 aatttctggt aaatgcccag ggagaagatg tggtggcggg aataaggaca cctcaaccca
     1081 tagaagaaat ggctgaggcc ttcccggaag tgtacaaaga attcatgagg atatcagaac
     1141 ttcttgaaaa acattataca gatatgcagg atatggaatt taccgttgaa agaaacaagc
     1201 tgtttatgct ccagacaaga aatggcaaaa gaacagctgc ggcggccgtg aagatcgcag
     1261 ttgatatgga acaagaaggg ctaataaata aaaagacagc cgtaacaaga ctggaaccgg
     1321 ctcagataga tcagttgctc catcctaaat ttgatgaggc agaactcaaa aaagcagaac
     1381 caataaccaa aggccttgca gcttcccccg gggcggcggc aggaaggata tacttcacgg
     1441 ccgaagaagc tgtggcagcg gtaaataacg gagaaaaggc aatactggta aggcttgaaa
     1501 cctcaccgga agatctggca ggcatggtag ttgccgaggg catacttacc gcaagaggcg
     1561 ggatgacatc ccatgcagca gttgtggcaa gaggcatggg aaaatgctgt gtttcaggct
     1621 gttccgagat aaaagtggaa gaagaagcaa agaaaatgac cgccggcggg aaggtattca
     1681 aagaaggaga tttcatttca cttgacggga gcgaaggtgt tgtctacaaa ggtcttataa
     1741 gtaccgtttc accggaactt tcaggtgatt tcggaatgat aatgaaatgg gcagatgaag
     1801 taagaacact gggggtaagg actaatgccg ataatcccag agatgccagg caggcggtgg
     1861 atttcggagc agaaggaata gggctttgca ggaccgagca catgttcttc gaagaagaaa
     1921 gaatatccaa tgtaaggcgg atgatacttg cagattccga agaagaaaga aggagtgccc
     1981 ttgcattgct tctcccttat cagaaaggcg atttcaaggg aatatacgaa gtcatggaag
     2041 acagacctgt taccataagg ctgcttgatc cgccgttgca tgaattcctg ccccatacag
     2101 aagaagaaat aaaggaactt tcaaaacaga taaatgtgcc ctatgaaaaa ttgtccaaaa
     2161 aagtcgaaga gcttcatgag ttcaatccta tgctgggaca cagaggatgc agacttgcgg
     2221 tcacatatcc cgagatcgct gagatgcagg ctgaagccat aataatggcg gcaattgaat
     2281 tgaaaaaaga aaagaaaatg gatattacac ctgaaataat gatacctctg gtgggccttg
     2341 aaaaggaact ggcatttgta aagcagactg tggtcgatac cataaacagg atatttgaaa
     2401 gagagggggt aaagcttgat tacatggtcg ggactatgat agagatacca agagcagcta
     2461 tcctggccga tgagatagca aaagaagctg agtttttctc atttggtaca aacgatctaa
     2521 cacaaatgac ctttggattt tccagggatg ataccggcaa cataatcaaa gaatacagag
     2581 acaaacttat ctttgaagaa gacccgttcc agacccttga tatttccggt gtcgggcagt
     2641 tggtaaaaat ggctgtagaa aaaggaaaac agacaagacc caatatcaaa cttggtatat
     2701 gcggagagca tggaggcgat ccgaagactg tgggattctg tcatgaaatc ggactcaatt
     2761 atgtttcctg ttcacctttc agggtaccca tagcaagact tgctgcagca caggctgcag
     2821 ttaaagcaga aaaaaagagt tgagaagcaa gaaaggagaa atcccatgaa agtgctgatt
     2881 tttaacggaa gtcccaggct taacgggaat accagaaccg cacttgttgc cttaaaagaa
     2941 gaaacagaac aaaaaatgcc ggattcagaa acagaactta ttgatgccta cagaaagaat
     3001 atcagcggat gcataaactg tgatgcctgc aaacacaatg gcggagagtg tgtccaggaa
     3061 gatgacacag ttgagataat tgaaaagata tatgatgcgg acatactgat atttggttct
     3121 cccgtatact ggtggggaat aacggcaaaa ctgaagctga taatagataa aatgtattca
     3181 aagtcagaac aactttccaa aacaaaaaag aaagtaggcg ttatagctgt gggtatggcg
     3241 ggacttacag atccggaata taagctaata acagatcagt ttcagtgtat ttgcgatcat
     3301 ctcaactggg agatgatgtt cagtgaagca tttgtttctg aaaatcccga ctccctcaaa
     3361 aagaataaag aaaaactaac ggaaataaaa gatcttcata gaaaattcat ataataagag
     3421 ccttttacag aaacgtacca aaaaataaag cttgaaaacc ttaaatatcg aaaacagcag
     3481 cagtttatgg ctgttgtttt cttttggtat attgacattt tttgccattt ggattattat
     3541 taatatcatg attacaaata ataacaaatg aggtgtaaaa tggatattat
//
LOCUS       DAONHK010000111         3588 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_20756_length_3588_cov_3.793660, whole genome shotgun sequence.
ACCESSION   DAONHK010000111 DAONHK010000000
VERSION     DAONHK010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3588
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20756_length_3588_cov_3.793660"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..788)
                     /locus_tag="PLM92_04685"
     CDS             complement(<1..788)
                     /locus_tag="PLM92_04685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HPR24465.1"
                     /translation="MTIKLIALDLDGTTLNSKGRLTQRTKNALEKAISKGINVVIATG
                     RCYSALPEEIIRICGIQYALTSNGAQIKDLCSGETLMSNCMDPFSVEESANVLSRYEY
                     MTEVFTDGKAYIEKKDYDEIARGGITYRYRDYVLRTRNPVEGAMDFMVDHKNSIENIN
                     IFFHDQEDKKRMWHVLSEISNVTVTSSLDNNWELGGATTSKAAALEDLCHILDVHKKE
                     IMACGDSLNDRDMLMEAGIPVAVGNAKDVIKEIASYITGTNNEDG"
     gene            complement(785..1372)
                     /locus_tag="PLM92_04690"
     CDS             complement(785..1372)
                     /locus_tag="PLM92_04690"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPR24466.1"
                     /translation="MEIETFTGGVLKSNGYVIYKKGKDTCYIIDPGYKANKFIGFVQK
                     NDLHCKGILLTHHHYDHVGVAEKTARETDCSVFIHRQDMDKYKSEAVAIEDGKIFLLD
                     EEEIRVVHTPGHTHGGVCYYSAKSKVVFTGDTVFNVDLGRTDLSDGSEEEMQDSIGNI
                     ISHWGNDMFIYPGHGDSCTMKYVRKNNKEFKEILK"
     gene            1527..2894
                     /gene="trkA"
                     /locus_tag="PLM92_04695"
     CDS             1527..2894
                     /gene="trkA"
                     /locus_tag="PLM92_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006001533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HPR24467.1"
                     /translation="MKIAIVGAGKLGHKVADALLGGDHSVTVIDKNEQTLQKLQSQMD
                     IMTVAANAKQISVLKDLHISSYDFLLAATDRDEKNIVIASFAKKLGCSKVIARVRDPE
                     HMNQLTFIRDTMSIDFTVNPDFAITMEIYKYLVEKYTLSNGIYTSGKVSFLEFYAEKM
                     PVLIDMPVSNFASVLPGMLLVAISRNGKVIIPHGNTVIEAKDCLYIVGEKVPVTVLSK
                     KVHEKGKWTDIQKVMIIGGGKTGLYLSQKLADFGASVKIIEQNKERCHYLSEHLEDVM
                     ILHGDGTDLALLEEENLDEIDAFVTATGYDEDNLLLALMAKSHHIEDVIAKVSRGIYT
                     DMISKMGVDMALNPLDITASNILRIIQGDKRVLSSQLIEGQAEIIEIVAEDHMKLTDK
                     TLKDMELPEGVIIAAIHRGFELIIPSGDTVIKEGDKVIALCLLSEVAAMEKLLCTKGV
                     TDFLK"
     gene            2908..>3588
                     /locus_tag="PLM92_04700"
     CDS             2908..>3588
                     /locus_tag="PLM92_04700"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HPR24468.1"
                     /translation="MDLNLRVLNQVTGILLLVSGCAMILPAVVAVIYGETSSLLAFVK
                     VLIPSIFTGVLFVIFARPKNVHMRIRDGFLIVSVCWLLLSLIGALPFVISGSIPNYID
                     AFFETCSGYSTTGASILADIESLPKSMLFWRSFTHWIGGMGVLVLAVALLPALGIGGQ
                     FIAKAESPGPTLSKLTPKISDTARLFYILYLIFTVTEIILLLIGGVNIYDAFIHTFGT
                     VGTGGFSNY"
BASE COUNT          965 a          788 c          731 g         1104 t
ORIGIN      
        1 ccgtcttcgt tgttggtgcc ggttatataa gatgctattt cttttatcac atcctttgcg
       61 ttgcccacgg ccacaggtat ccctgcttcc atgagcatat ctctgtcatt taaactgtct
      121 ccgcatgcca tgatctcttt tttatggaca tccaaaatgt gacaaaggtc ttccagtgcc
      181 gctgctttgc tggtagtcgc gcctcccagc tcccagttgt tgtccagaga agatgtaaca
      241 gtcacatttg atatttccga aagcacatgc cacattcttt ttttgtcttc ctggtcatgg
      301 aaaaatatat ttatattttc tatggaattc ttatggtcga ccatgaaatc catggctcct
      361 tccacaggat tacgggtcct gagtacgtaa tccctgtatc tgtaggtgat gcctcccctg
      421 gctatttcat catagtcttt cttttctata tatgctttgc cgtcagtaaa aacttctgtc
      481 atatattcat agcgactaag tacatttgca gactcctcaa ctgaaaaagg gtccatgcag
      541 ttgctcatta gagtttcgcc gctgcacaga tcttttatct gggccccgtt tgatgtcagc
      601 gcatattgta tcccgcatat tcttattatt tcttcaggca gagcagaata acatctacct
      661 gtggcaataa ccacatttat cccctttgat atggcctttt ccagggcatt ttttgttctt
      721 tgtgtgagcc tgcctttgct gtttagtgtc gtcccgtcca gatcaagtgc aatgagcttt
      781 atggtcattt tagtatctcc ttgaattctt tgttgttttt tcttacatac ttcatcgtgc
      841 aggaatcgcc atgaccggga taaatgaaca tatcatttcc ccaatgggat ataatgtttc
      901 cgatgctatc ctgcatttct tcttctgagc cgtcggaaag atcagtgcga ccgagatcta
      961 cattgaatac agtatctccg gtaaaaacga ccttgctttt tgcggaataa taacataccc
     1021 ctccatgagt gtgtcccggt gtatggacga cccttatttc ctcctcatca agcaaaaaaa
     1081 ttttaccgtc ttctatggcc acagcttcac ttttatactt atccatatcc tgtctgtgta
     1141 taaaaacaga gcagtctgtt tcacgggcgg ttttttccgc aacacccaca tggtcatagt
     1201 ggtgatgagt caacagtatt cctttgcagt gcagatcgtt tttctgcaca aaccctatga
     1261 atttatttgc tttatacccc ggatctatta tataacacgt gtcttttcct tttttatata
     1321 tgacatatcc attgcttttc agcactcctc cggtaaatgt ttctatttcc atttcttcat
     1381 tccagccttt cttttttata atgtcctcaa atcattgcgt tctgctatat tttattgtat
     1441 ttcaagacaa catgcaaata aatgttgaaa aacaatttaa tagtggtaga atgtgcttaa
     1501 atgcaaaaac agaaaggtat attccaatga aaatcgccat cgtaggagca gggaaactgg
     1561 gtcacaaagt ggcagatgcc ctgcttggcg gagatcactc cgtgacggta atagataaaa
     1621 acgagcagac actccaaaaa ctgcagtctc agatggatat catgacagtt gcagcaaatg
     1681 caaagcagat atccgtccta aaggatttgc atatttcttc atatgatttc cttctggccg
     1741 ccacggacag agacgaaaaa aacatagtaa tagcttcctt tgcaaaaaaa ctgggctgtt
     1801 ccaaggttat agcaagggta agggatccgg agcatatgaa tcagctgact tttatcagag
     1861 atacaatgtc catagacttc actgtgaacc cggattttgc cataaccatg gaaatatata
     1921 aatatcttgt tgaaaaatat actttgagca atggtatata taccagtggc aaagtctctt
     1981 ttcttgagtt ttatgccgag aaaatgcctg ttcttattga tatgcccgta tcaaactttg
     2041 catcggtgct tcccggtatg ctgctggtgg ccatctcaag aaacggcaag gttataatcc
     2101 cccacggaaa tacagtaata gaagccaaag actgtcttta tatagtggga gaaaaggtcc
     2161 ctgtaacggt cctcagcaaa aaagtccatg aaaaaggcaa atggacggat atccaaaaag
     2221 tcatgatcat aggcggagga aaaacaggtc tttatctttc acagaaactt gctgatttcg
     2281 gggcttccgt aaaaataata gaacagaaca aagaacgttg ccactacctt tcggaacatc
     2341 ttgaagacgt tatgatcctc cacggagacg ggactgatct tgctttgctg gaggaagaaa
     2401 atcttgacga aatcgatgcc tttgtcactg ccacagggta tgacgaagac aatctgctcc
     2461 tggcgctcat ggcgaaatcc caccatatag aagatgtgat cgccaaggtg agccgcggta
     2521 tatatacgga catgatatcg aaaatgggtg ttgatatggc ccttaatccc ctggatataa
     2581 cagccagtaa tatcttaagg ataatacagg gagacaagcg tgtactgtct tcccagctca
     2641 tagaaggtca ggcagaaata attgaaatcg ttgcagaaga tcatatgaaa ctgacagaca
     2701 aaacccttaa agatatggaa cttcccgagg gagtaataat agctgccata cacaggggct
     2761 tcgaactgat aatcccgtca ggcgatactg taataaagga aggcgacaag gtaatagcac
     2821 tttgtctttt atccgaagtc gccgccatgg aaaagctgct ttgtacaaaa ggagtgacgg
     2881 atttcctgaa ataggagatg ttgactgatg gatctgaatc ttcgtgttct taatcaagtg
     2941 accggcatcc ttttgctcgt gtccggttgc gctatgattt tgccggctgt agtggcagtt
     3001 atatatgggg agacatcaag ccttttagcc tttgtaaagg ttctgatacc ctctatattt
     3061 acaggtgttc tttttgtcat atttgccaga ccgaaaaatg tacatatgcg cataagggac
     3121 ggctttctca tagtgtccgt atgctggctc ctgctttcat tgataggtgc acttcccttt
     3181 gttatttccg gaagcattcc aaattatatt gatgcttttt ttgaaacatg ctccggatac
     3241 agtacgacag gtgcatcgat acttgctgat attgaatcac tgccaaaatc catgcttttc
     3301 tggcgttcat tcacccactg gataggggga atgggtgtgc tggtcctggc cgtcgctctg
     3361 cttcccgcac tgggtatcgg aggacagttt attgcgaagg ccgaatctcc cggtcccacc
     3421 ctttcaaagc tgacccctaa gatatcggat acggcaagac ttttctatat cctttacctt
     3481 atattcaccg taactgaaat catccttctt cttataggtg gagttaacat ttatgatgca
     3541 tttatacaca ctttcggtac agtcggcacc ggtggttttt ccaattat
//
LOCUS       DAONHK010000112         3563 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_20973_length_3563_cov_4.425599, whole genome shotgun sequence.
ACCESSION   DAONHK010000112 DAONHK010000000
VERSION     DAONHK010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3563
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20973_length_3563_cov_4.425599"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /locus_tag="PLM92_04705"
     CDS             <1..581
                     /locus_tag="PLM92_04705"
                     /inference="COORDINATES: protein motif:HMM:NF025300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HPR24469.1"
                     /translation="LVLRQDNADIRLTEKSYILGLASKERYKRVIRKKEIIGKEEKRL
                     ETTRIDRETANSFLKKTGGAGVKNSVTLSELLKRPEVSYERLKEIDRERPEMKKHEAE
                     ELQVRIKYKGYIEKQLAQIERFRKMEDKKLDENMNYREIQGIREEAKQKLEALKPLSL
                     GQASRISGVSPADINVLMIWLEKKKREKKESK"
     gene            578..1294
                     /gene="rsmG"
                     /locus_tag="PLM92_04710"
     CDS             578..1294
                     /gene="rsmG"
                     /locus_tag="PLM92_04710"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HPR24470.1"
                     /translation="MKKIKNLLRCFEIEENIIKTAVPAMEEYMNSILEWNKKVNLTAI
                     TSREEFIDKHLADSLMCAGSNEIQNSKTIIDVGTGAGFPGVPLALIFPEKSFLLLDSQ
                     NKKIEIIKEEAQKVGIRNAVFIQGRAEDLAGEEDYREKFDICVSRAVAPMAVLLEYCL
                     PFVRTGGNFLAYKGAKWQQELYAAENALDILGGEKIRIDSSNFTKEDTDHVVIYIYKE
                     KETPRKYPRKAGKPSKYPLL"
     gene            1390..2166
                     /locus_tag="PLM92_04715"
     CDS             1390..2166
                     /locus_tag="PLM92_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPR24471.1"
                     /translation="MGKAIAIFNQKGGVGKTTTNINLAASLAQKGKKVLVIDIDPQGN
                     TTSGLGISKKTLEISTYELLTDDDANAKDAIMHTSTENMDIIPANAQLAGAEVELVQV
                     KAREKRLKQAIEGIKEAYDYIFVDCPPSLGLLTINSLTAVDSVLVPIQCEFYALEGVS
                     QLMSTIEIVRKNLNPELQIQGVILSMFDGRTNLSIQVVEEVKKYFKEKVYTTVIPRNV
                     RLAEAPSYGMTIAEYDPRSTGAEAYREFAEEFLEMEESHG"
     gene            2159..3034
                     /locus_tag="PLM92_04720"
     CDS             2159..3034
                     /locus_tag="PLM92_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005999148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HPR24472.1"
                     /translation="MAKPKNKGLGRGLEALMGEASIGIREKDGEERGIEYIDINNIKP
                     NRNQPRKVFSEEKINELADSIKEHGVIQPLIVRTAESGYELVAGERRWRAARTAGLKE
                     VPCILRKLTEEENMLLAVVENMQREDLNPIEEAEGLGNMIEAFGLTQEQISKSVGKSR
                     PYITNALRLLKLPDEIKEMISSGKISQGHGRVLLSVSDRKKQFDLAKRIVKEDLSVRV
                     TEALAEEKKEKGKKTKARKMTKNPDVLQIERELKDALGTKVSINMKGKKGRIEIEYYS
                     KEELERLIDLLKEKK"
     gene            3269..>3563
                     /locus_tag="PLM92_04725"
     CDS             3269..>3563
                     /locus_tag="PLM92_04725"
                     /inference="COORDINATES: protein motif:HMM:NF012447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HPR24473.1"
                     /translation="MAILINGKKLTVEEVIRVARGGEEVRICEEAKIAVKKARDYVDK
                     KLSEGAIIYGLTTGFGKFSDTFISCEETADLQRNLIISHTCALGQPLPKETVRA"
BASE COUNT         1363 a          615 c          842 g          743 t
ORIGIN      
        1 ggcttgtttt aagacaggat aatgcagata taagacttac ggaaaaatcc tacatacttg
       61 gactggcatc aaaagaaaga tacaaaagag tcattcgaaa gaaggaaatc atcggaaaag
      121 aagaaaaaag gcttgaaact actcgaatag accgggaaac agcaaattct ttcctaaaga
      181 aaacaggagg cgccggtgtc aaaaacagcg ttacactttc agaacttcta aaaagaccgg
      241 aggtttcata cgagcgttta aaagagatag accgggaaag accggagatg aaaaagcatg
      301 aggcagaaga actccaggtc cgcataaagt ataagggata tatagaaaaa caactggcac
      361 agatcgaacg tttcagaaaa atggaagata aaaaacttga tgaaaacatg aattaccgcg
      421 agatccaggg catcagggaa gaagcaaaac aaaagctgga agcattaaaa cccctttccc
      481 tgggacaggc atcaagaata tcaggagtat cccccgcaga cataaatgtt ctaatgatat
      541 ggcttgaaaa gaaaaaaaga gaaaaaaagg aatctaaatg aaaaaaatca aaaacctgtt
      601 aagatgtttt gaaatagaag aaaatataat caaaaccgct gttcccgcca tggaagaata
      661 tatgaacagc atccttgagt ggaataaaaa ggtaaatctt acagcaataa ccagcaggga
      721 agaatttatt gacaaacatc tggcagattc ccttatgtgt gcaggctcaa atgagatcca
      781 aaactcaaaa actattattg acgtgggtac aggagccggt tttcctggtg taccgctggc
      841 attgattttt ccggaaaaaa gttttttgct tctggattca caaaataaaa aaatcgaaat
      901 aataaaagag gaagcacaaa aagtcgggat cagaaacgcc gtattcatac aaggacgtgc
      961 ggaagatctg gccggagaag aagattacag ggaaaaattc gacatttgtg tatcaagggc
     1021 ggttgcgcca atggctgtgc ttttggaata ctgccttccg tttgtcagga caggcggaaa
     1081 ttttttggct tataaaggag cgaaatggca gcaggagctg tatgccgcag aaaatgctct
     1141 ggatatactg ggcggggaaa aaataaggat cgacagcagc aactttacca aagaagatac
     1201 agatcatgtt gtgatatata tctacaaaga aaaggaaacc ccccggaaat atccccgaaa
     1261 ggcaggaaag ccttccaaat acccgctttt ataaaaatgt ttcacgtgaa acattttttt
     1321 attgtggcat attgccatag tataatgtat aatcaaagag cactgagcac aaagaaagga
     1381 ggcgaggaat tgggtaaagc gatagctatt tttaatcaga aaggtggcgt tggcaaaacc
     1441 acaaccaata taaatcttgc cgcaagtctt gcacaaaaag gaaaaaaagt actcgtgata
     1501 gatattgatc cccagggaaa tacgaccagc ggactgggaa tatccaaaaa aacccttgag
     1561 atttcaacat atgagttgct tacagatgat gatgcaaatg caaaagatgc catcatgcat
     1621 acatctacgg agaatatgga tataatcccg gctaacgcac agctggccgg agcagaagtg
     1681 gaacttgtac aggtcaaagc cagagaaaaa agactgaagc aggccatcga aggcataaag
     1741 gaggcatatg actatatttt tgtggactgt cccccttctc tcggattatt gactatcaac
     1801 tctcttacgg ccgtggattc cgtacttgtt cccatacagt gcgagtttta tgctcttgag
     1861 ggcgttagtc agttgatgag taccattgaa atagtaagga agaatcttaa tccggagctt
     1921 caaatacaag gagttatcct gagtatgttc gatggaagaa caaatctttc tatacaggta
     1981 gtggaggaag tcaaaaagta tttcaaagaa aaggtttaca caacggtcat accaagaaat
     2041 gtgaggctgg cagaagcccc cagttatggc atgaccattg cggaatatga ccccagatcc
     2101 acgggagctg aagcttacag ggaatttgcg gaagaattcc tggagatgga ggaaagccat
     2161 ggctaaacca aagaacaaag ggttgggacg tggtcttgag gccctgatgg gagaggcatc
     2221 cattggcatc cgtgaaaaag acggagaaga aagaggaata gaatatatag acataaataa
     2281 cataaagccc aacagaaatc agccaagaaa agttttcagc gaagaaaaaa taaacgaact
     2341 ggcggattcc ataaaagagc acggagtcat acagcctctt atagtaagaa ccgccgagtc
     2401 cgggtatgaa ctggtggcag gggaaagaag atggcgggca gcaaggacag cgggccttaa
     2461 agaagtgccg tgtatactca gaaaactgac agaagaagaa aacatgctgc tggccgttgt
     2521 ggaaaacatg cagcgggagg atctgaatcc tatagaagaa gccgaaggac tgggaaacat
     2581 gatcgaagcg ttcggactta cacaggagca gatatcaaaa agtgtgggaa aaagcagacc
     2641 ctatataaca aatgcactgc gtttacttaa attgccggac gaaataaaag aaatgataag
     2701 cagcggaaag atctcccagg gacatggaag ggtgctgctt tcggtgtcag acagaaaaaa
     2761 acagtttgat ctggctaaga ggatagtgaa agaagacctt tctgtgcgtg tcaccgaagc
     2821 tctggcggaa gaaaaaaaag agaagggcaa aaaaacaaaa gccagaaaaa tgacaaaaaa
     2881 ccccgatgtt ctgcaaatag aaagggaatt gaaggatgca ctgggaacaa aagtcagcat
     2941 aaacatgaag ggcaaaaaag gcaggatcga aattgaatac tacagcaaag aagaactgga
     3001 aagattaata gatcttctca aagaaaagaa ataaaaatgt ttcatgtgaa acatacaccg
     3061 gaaagaatgg ccgatcagag aagatcggcc atttgcgtta ataaatatac acaatataac
     3121 tgtatcacaa caaagggata aagcgttgat aaatttcaaa aaactatgga aaaaaagtga
     3181 atgattaagt ataatcaaaa aaaggctatc acgaagaaaa tgtatattta tacacaataa
     3241 taaatattta tagaacggag acaaaaagat ggcaatactt atcaatggaa aaaaactgac
     3301 agttgaggaa gttatcaggg tcgcccgcgg gggggaggaa gttcggatct gcgaagaagc
     3361 aaaaatagca gtaaaaaaag cacgggatta tgttgacaag aaactttctg aaggagcaat
     3421 aatttatggt cttaccaccg gctttggaaa attttccgat acatttattt catgtgaaga
     3481 aacagcggac cttcaaagaa atcttataat aagtcacaca tgtgcactgg gacagcctct
     3541 gccaaaagaa accgtacggg cag
//
LOCUS       DAONHK010000113         3549 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_21081_length_3549_cov_5.777333, whole genome shotgun sequence.
ACCESSION   DAONHK010000113 DAONHK010000000
VERSION     DAONHK010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3549
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21081_length_3549_cov_5.777333"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(37..381)
                     /locus_tag="PLM92_04730"
     CDS             complement(37..381)
                     /locus_tag="PLM92_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPR24474.1"
                     /translation="MGQMSLPLDSWSKTKIGLEQRQTLTREVIEEYQIKKIRDTFELA
                     VANSPFYRGIYGDLPVPESLEDMADIPFTMPKDLAFHGEEMICVPQKEISRIITMETS
                     GTTGTAKRVFFT"
     gene            complement(369..743)
                     /locus_tag="PLM92_04735"
     CDS             complement(369..743)
                     /locus_tag="PLM92_04735"
                     /inference="COORDINATES: protein motif:HMM:NF021252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HPR24475.1"
                     /translation="MNNEIMDYKLKGYCCSQIIMEMGLKRLGLEDKEMIKAMRGLCFG
                     MNHGKNCGILTAAMCLISLACTEETRARCGDDFFDWFEDTFGAIDCEVLLEGDPMNKV
                     EKCPGMIEASFAELSDILEWDR"
     gene            complement(745..1464)
                     /locus_tag="PLM92_04740"
     CDS             complement(745..1464)
                     /locus_tag="PLM92_04740"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPR24476.1"
                     /translation="MFRPGSFDITDKAIEKMALPKEAKVLEIGCGEGETVERLEKKYG
                     YRLTAIDKSLEMVSKAKKRGLKADIKYGDGEFLEDIPSLSLDAVIMECVLSRIKLADE
                     ALHEAYCVLKKSGKLFISDIYMKDPDPEKVKAIKIEAERQSKIMEPYPAPGEHHDPSK
                     VHDEETCSDECAEDHRKRFYDFRFDGLFIKEPLIRAVEDIGFKVTFWEDRTADLIEYT
                     KENFMRPEGVSYFMLIAEKGD"
     gene            complement(1442..2875)
                     /locus_tag="PLM92_04745"
     CDS             complement(1442..2875)
                     /locus_tag="PLM92_04745"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPR24477.1"
                     /translation="METIQKTQTICPVCGRPLSAEYKKEKDGVYFISECPEHGITKTL
                     AAENEKDITEWMGSPVVNVPPKRPITKGNQNECPLHCGTCENHMQTACCVLIDITNRC
                     NQHCPYCFAVSYLSEGRRAKEGEPDFAEMERKYDLLLELGEERPFNIHISGGEPTVRD
                     DLPEIIRMGREKGFEYIQVNSNGKRIAEEENYAKILKEAGTSVVFMQFDGTREDIYLK
                     LRNEALFEKKKKAVENCRKAGLPVTLVPTIVKGINLDNIGDMMKYLLDNTDVIKGIHF
                     QPASFFGRYPGEDEKTRRATMFDVLHGIEEQAEGFCYKDFAPIATGHQLCCFYSTYMK
                     MPDGKVKLMLSKEEKDKGVTCCGKSEDKEISCCSKEPSEYEKIDIIKKNRDYVLNKWS
                     VAGLGEECSCKDPENMDFDEFLSYYKNNVFTVTGMAFMDRSNLDAERLKRCRVQQLTE
                     DDRLIPFCAYNSYYRDKEKKDVQTRKF"
     gene            complement(2920..>3549)
                     /locus_tag="PLM92_04750"
     CDS             complement(2920..>3549)
                     /locus_tag="PLM92_04750"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007060558.1"
                     /note="NAD-dependent; catalyzes the formation of
                     3-phospho-D-glyceroyl phosphate from D-glyceraldehyde
                     3-phosphate; active during glycolysis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HPR24478.1"
                     /translation="VYGVNHDILTEKDNIISGASCTTNCLAPMAKVLNRYRELRTGFM
                     TTIHAYTGDQMILDGPHRKNDFRRARAGAINIVPNSTGAAKAIGLVIPELAGKLIGSS
                     QRVPVPTGSLTILDATLKDETETVSVEGINEAMKAAASESFGYTEEELVSSDVIGMHY
                     GSLFDATQTLAERCGTQIYEVRVVSWYDNEMGYVCQLIRTLYHIGKNFI"
BASE COUNT          834 a          875 c          639 g         1201 t
ORIGIN      
        1 gtgatgaaaa taatctattg taagttcctg atcttcttac gtaaaaaaca ctcttttggc
       61 agtgcctgta gtcccgctgg tctccattgt aattatcctg ctgatttcct tttgcgggac
      121 acatatcatc tcttctccgt gaaaagcaag atcttttggc attgtgaaag gaatatctgc
      181 catatcttca aggctttcag ggacaggaag atccccgtat attcctctgt aaaaaggact
      241 gttggcaaca gcaagttcaa acgtgtctct tatctttttt atttgatact cctcgatgac
      301 ttctctggta agtgtctgtc tctgttccag tcctatcttt gtttttgacc atgaatcaag
      361 aggaagactc atctgtccca ctccagaatg tccgaaagct ctgcaaaaga tgcttctatc
      421 atgcccggac atttttcgac tttgttcata ggatctcctt ccaacagcac ttcacagtct
      481 attgctccaa atgtgtcttc gaaccagtcg aaaaaatcat ctccgcaacg ggctcttgtt
      541 tcttctgtgc aggccagaga tataagacac atggcggctg taagtatacc acagtttttt
      601 ccgtgattca taccgaaaca aagtcctctc atggctttga tcatttcttt atcttcaagg
      661 ccaagtcttt taagacccat ttccattatt atctgactgc agcaatagcc cttaagttta
      721 tagtccatga tctcattatt cattttagtc ccctttctct gcaataagca tgaagtaact
      781 taccccttcc ggtctcatga aattttcttt tgtgtattct ataagatccg ccgtccggtc
      841 ttcccagaaa gtcactttaa atccgatatc ctccaccgct cttataaggg gttcttttat
      901 aaaaagtccg tcaaaccgga aatcgtaaaa cctttttctg tgatcttcgg cacattcgtc
      961 actgcaagtt tcttcgtcat gtaccttgct gggatcatgg tgttctcccg gtgcaggata
     1021 tggttccata atttttgact gcctttctgc ttctatcttt atcgccttca ctttttcagg
     1081 gtccgggtct ttcatgtata tatcgcttat gaaaagctta cctgattttt ttaaaacaca
     1141 gtaggcttca tgcagcgctt catccgccag ttttatgcga gaaaggacgc attccatgat
     1201 taccgcatcc agggaaagtg aaggaatatc ttcaaggaat tctccgtcac cgtattttat
     1261 gtccgcctta aggcctcttt tttttgcctt gcttaccatt tccagggatt tatctattgc
     1321 agtcagtctg tatccgtatt ttttttccag gcgctcaacg gtttctccct ctccgcagcc
     1381 tatttccagc acttttgctt ccttcggcag cgccattttt tcgattgctt tgtctgtaat
     1441 atcaaaactt ccgggtctga acatcttttt tctccttatc acgataataa ctgttatatg
     1501 cacaaaatgg aatcaacctg tcatcttctg taagctgctg gactctgcag cgtttcagtc
     1561 tctcagcatc caggttgctt cgatccataa atgccatacc tgtaactgta aacacattgt
     1621 tcttataata actcaaaaac tcatcaaaat ccatgttttc gggatctttg cagctacatt
     1681 cctcacccag tcctgccacg gaccatttat tcaaaacata gtctctgttt ttctttatta
     1741 tatcgatttt ctcatattcc gaaggctcct ttgaacagca ggatatttct ttgtcttcag
     1801 atttaccgca acatgtaact cccttatctt tttcctcctt tgagagcata agttttactt
     1861 tcccgtcagg catcttcata tatgtgctgt aaaagcagca taactgatga cctgtggcta
     1921 tgggagcaaa atctttataa caaaaacctt cagcctgttc ttctataccg tgcagcacat
     1981 cgaacattgt ggcccggcgg gttttttcgt cctctccggg ataacgcccg aaaaaacttg
     2041 cgggctgaaa gtgtattcct tttatcacat ccgtattatc aagaagatat ttcatcatat
     2101 ctcctatatt atccagattt attcccttga ctatggtggg taccaaagta acaggcaaac
     2161 cggctttcct gcagttttca acggcttttt tctttttttc aaaaagagcc tcgttacgaa
     2221 gctttagata tatatcttct cttgtgccgt caaactgcat aaatacaaca gaagtaccgg
     2281 cttccttaag gatcttcgca taattttctt cttcagcaat gcgttttccg ttgctgttta
     2341 cctgtatgta ttcgaatccc ttttcacgcc ccatccttat gatctccggc agatcgtctc
     2401 ttaccgtagg ttctcctccc gatatgtgta tattgaaagg tctttcttcc cccagttcca
     2461 ggagcagatc atatttccgt tccatctctg caaaatccgg ttccccttcc tttgcccttc
     2521 ttccttcaga caaataggat actgcaaagc agtaggggca gtgctgattg caccggtttg
     2581 ttatatcaat aagcacgcaa caggctgtct gcatgtgatt ttcacatgta ccgcaatgca
     2641 gagggcactc attttgattt ccctttgtta taggtctttt gggcggtaca tttaccaccg
     2701 gggaccccat ccattccgtg atatcctttt cattctctgc ggcaagggtc tttgttatgc
     2761 cgtgctcggg acattcactg atgaaatata ccccgtcttt ttcttttttg tattccgcag
     2821 acaaaggtct tccgcaaacc ggacatatgg tttgtgtttt ttgtatcgtc tccatttgtt
     2881 actgtcttaa ctccttgcag tgtcatataa gcattttatt tatataaagt ttttccctat
     2941 atggtaaagg gtcctgatca gctggcagac ataacccatc tcattgtcat accatgatac
     3001 tactctcacc tcgtatattt gcgtaccaca cctttctgcc agggtctggg tagcatcaaa
     3061 aagtgagccg taatgcatcc caataacatc actggaaacc agttcttctt cagtataacc
     3121 gaatgactcc gatgccgctg ctttcatggc ttcatttatt ccttccacag aaacagtttc
     3181 agtctcgtct ttcagagtag catccagtat agtaagagag cctgtaggca ccggcacccg
     3241 ctgagaggaa cctatcagtt tgcctgcaag ttcgggtatg acaagcccga tggcttttgc
     3301 agccccggtg ctgtttggca caatatttat agctcccgcc cttgctcttc tgaaatcatt
     3361 ttttctgtgg ggaccgtcaa gtatcatctg atctccggta tatgcatgta tagtcgtcat
     3421 aaagcccgtc ctcagttctc tgtaccggtt aagtactttt gccatggggg caagacagtt
     3481 tgtggtgcag gaagctcctg aaattatgtt gtctttctct gtcaaaatat cgtggttgac
     3541 accatagac
//
LOCUS       DAONHK010000114         3480 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_21685_length_3480_cov_4.052847, whole genome shotgun sequence.
ACCESSION   DAONHK010000114 DAONHK010000000
VERSION     DAONHK010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3480
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21685_length_3480_cov_4.052847"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..624)
                     /gene="trmB"
                     /locus_tag="PLM92_04755"
     CDS             complement(<1..624)
                     /gene="trmB"
                     /locus_tag="PLM92_04755"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF001080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HPR24479.1"
                     /translation="MRQRKLKNLDERLGYFKNWIVSEPEKQKGRWKEAAREGVPAGKQ
                     NVSIRLEIGCGKGRFLTEKAINDAEKKYIGIEGQQSVIVRAIEKAEKADLKNIMFISG
                     YVRDLREIFADGEVEGIYLNFSDPWPKKRHACRRLTHRNFLVSYKAVLAPGGFIEIRT
                     DNKDLFDFTLSDAEATGFFVENVTEDLYGSDIIKDGIMTEYEEKFSSM"
     gene            complement(641..1108)
                     /locus_tag="PLM92_04760"
     CDS             complement(641..1108)
                     /locus_tag="PLM92_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011971165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HPR24480.1"
                     /translation="MKTKYLIQAGLIAALYAALTVLLGAISYGPVQLRIAEAMTVLPA
                     LTPAAVPGLFAGCLISNMLGPYGALDMITGSLATLAAAFCSYKLKEKPLLVPLPPVII
                     NGIVIGSMLYYVYGVPFSLAACIGWVTLGQFMSCYILGYPLLLVFRKHKNIFE"
     gene            complement(1105..>1290)
                     /locus_tag="PLM92_04765"
     CDS             complement(1105..>1290)
                     /locus_tag="PLM92_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016287068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HPR24481.1"
                     /translation="PLFISAFRIAQDMAMAMEARCYHGGENRTKMNGMKFAKRDAFAA
                     FLMLLFLAVIILGRVFL"
     assembly_gap    1291..1390
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1391..2060)
                     /locus_tag="PLM92_04770"
     CDS             complement(<1391..2060)
                     /locus_tag="PLM92_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005341141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HPR24482.1"
                     /translation="MLRDITLGQYYSGESWIHRLDPRVKIIATLLFIVEIFIVNSFEG
                     FATAFAALIIVIAASKVPLKFIMRGLKPIFLIILFTFIVNLFIVEGEILVKLWIFTIT
                     KEGLYRAVFMAVRLILLIIGSSLLTLCTKPMSLTDGLESLMKPFKMIGVPAHELAMMM
                     TIALRFIPTLIEETDKIMKAQQARGADFETGNIFRRAKHLIPILVPLFISAFRIAQDM
                     AMAME"
     gene            complement(2054..2908)
                     /locus_tag="PLM92_04775"
     CDS             complement(2054..2908)
                     /locus_tag="PLM92_04775"
                     /EC_number="3.6.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005366617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HPR24483.1"
                     /translation="MSISAQKINHIYSKGLADQTVALKDVSFDINDGEFVGIIGHTGS
                     GKSTLLQHLNGLLKPDSGRIFINDREITAEGVNMMEIRRGTGLVFQYPEYQLFEETVE
                     KDIAFGPLNLGISQEETKARVREALSMVGLDPCEVAEKLPFDLSGGEKRRVAIAGVIA
                     MKPKVLILDEPTAGLDPKAHKEILDMIEEIHKNEKNITILVSHKMDDIARLCDKILVM
                     EKGTVVMTGTPEEIFSKGEELQGMGLDLPEAAQLILELRKRGIEKGETVLTMEQAERI
                     LLEVLGKC"
     gene            complement(2884..>3480)
                     /locus_tag="PLM92_04780"
     CDS             complement(2884..>3480)
                     /locus_tag="PLM92_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPR24484.1"
                     /translation="QTAGMVFQNPDNQIVSSIVEEDVAFGPENLGISPSEIRKRVEDS
                     LSAVNMGDYRKKPPHLLSGGQKQRIAIAGVVAMKPECIIFDEPTAMLDPKGRGEVMDI
                     IGELNQEGITVILITHFMEEAAMADRVIIMDEGRIKLDGSPSSVFAKRDELKSMSLDV
                     PFVVELAHRLRQKGVDIPENTVSREDLVEYVCRSVHKK"
BASE COUNT          842 a          862 c          645 g         1031 t
ORIGIN      
        1 cattgaagag aatttttctt cgtattctgt cattatcccg tcttttataa tgtccgaacc
       61 gtaaaggtct tctgtaacat tttccacaaa aaatcctgtt gcttcagcat cggaaagggt
      121 gaaatcaaaa agatctttat tgtcggtcct tatctcgatg aaacctcccg gggcaagaac
      181 agctttgtaa cttacaagaa aattcctgtg ggtgagcctt cggcaagcgt gccttttctt
      241 tggccatgga tcgctgaagt tcagatatat cccttcgact tctccatctg caaatatttc
      301 cctcagatcc cggacataac cagatataaa cattatattt ttcaggtccg ctttttcagc
      361 cttttcgatg gcacgaacta ttacactctg ctgcccctct ataccgatat attttttttc
      421 ggcatcattt atggcttttt cagtaagaaa acgtcccttt ccgcatccta tctccagcct
      481 tatgcttaca ttctgttttc ccgccggaac accttccctt gccgcttcct tccatcgtcc
      541 cttttgtttt tccggttcac tgactatcca gtttttgaaa tatcccagtc tttcatcaag
      601 gttttttagt tttctctgtc tcatgatctc tctttattta tcattcaaaa atatttttgt
      661 gtttacggaa taccagaagc aaaggatatc ccagtatata acatgacatg aactgaccca
      721 gtgtcaccca gcctatacat gctgccagac tgaagggtac accgtaaaca taataaagca
      781 ttgaacctat gactatcccg tttattatga ccggtggcag tggcaccagc agaggttttt
      841 ccttcagttt ataagagcaa aaagccgccg ccagtgtagc aaggcttcct gtgatcatat
      901 caagggctcc gtatggcccc agcatattgc ttataaggca gccggcaaaa agtccgggga
      961 ctgcagccgg cgtcaaagcc ggcaaaaccg tcattgcttc agcaattctc agctggaccg
     1021 gcccgtagct tatggctccc agcaatactg taagtgccgc atataaagct gcaataaggc
     1081 ctgcttgtat aagatatttg gtcttcatag aaacactctt cccaatatga tcactgccag
     1141 gaaaagcaac atcagaaaag cggcaaaggc atctcttttg gcaaatttca taccgttcat
     1201 ttttgtcctg ttttccccgc catgatagca tctggcctcc atggccatgg ccatatcctg
     1261 agctatcctg aaagcgctta taaaaagcgg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn cctccatggc catggccata tcctgagcta tcctgaaagc gcttataaaa
     1441 agcgggacaa gtatgggtat caggtgtttt gcccggcgaa aaatattgcc ggtctcaaaa
     1501 tcggctcctc ttgcctgctg agctttcatt atcttgtcag tttcttctat aagtgtggga
     1561 atgaaccgca gggctatagt catcatcatt gccagttcat gagccgggac tccaatcatt
     1621 ttgaagggct tcatgaggct ttccagtccg tctgtcaaac tcatgggttt tgtgcaaagg
     1681 gtaagcagag aagaacctat tatcaaaagt ataagtctta ctgccatgaa taccgccctg
     1741 taaagacctt ccttcgttat tgtgaatatc cataatttta ccagtatctc accttctact
     1801 atgaaaagat tgactatgaa agtgaaaagt atgataagaa atatgggttt aagtcccctc
     1861 ataataaatt tcagcgggac cttggaggct gcaataacta tgatcagtgc agcaaaggct
     1921 gttgcaaagc cctcaaaact gtttactata aaaatttcaa ctataaataa aagagtggct
     1981 atgatcttta ctcttggatc aagtctgtgg atccatgact ctcccgaata atactgtccc
     2041 aatgttatgt ctcttaacat ttgcccagca cctccagtaa tatccgttca gcctgctcca
     2101 tcgtaagaac tgtttcgcct ttttctattc ctcttttcct gagctccaga atgagttgtg
     2161 cggcttcggg aagatcaagt cccattccct gaagttcttc tccctttgag aatatttcct
     2221 cgggggtacc cgtcatgaca acggttccct tttccatcac cagtatcttg tcgcaaagcc
     2281 tggctatatc atccatcttg tgggaaacga gtatggtaat gtttttttca tttttgtgga
     2341 tctcctctat catgtccagt atttctttgt gggccttggg atccagtcct gcagttggct
     2401 catccagaat gagcaccttg ggtttcatgg cgataacacc ggcaatggcg actcttcttt
     2461 tttctcctcc cgaaagatcg aagggtaatt tttccgccac ttcacatgga tcaagtccga
     2521 ccatggaaag ggcctcccga acacgggctt ttgtctcttc ctgagaaatt cccagattca
     2581 ggggtccgaa agcgatatcc ttttctaccg tctcctcaaa aagctgatat tccggatact
     2641 gaaaaaccag tcctgtcccc cgacggatct ccatcatatt cacaccttct gccgttattt
     2701 ctctgtcgtt aataaaaatc ctgccggaat caggtttcag gaggccgttc aaatgctgca
     2761 gcaatgtgga ctttcccgaa cccgtatgcc cgattattcc cacaaactca ccatcattta
     2821 tgtcaaagct gacatcctta agtgccacag tctgatccgc cagtcccttg ctgtatatat
     2881 gatttatttt ttgtgcactg atcgacatac gtattccacc aaatcttctc tgcttactgt
     2941 attttcaggg atatcaacac ctttttgtct caaacgatgc gccagttcaa ctacaaaggg
     3001 aacatccaga ctcattgact tcaattcatc cctttttgca aacacgcttg aaggactgcc
     3061 gtcaagtttg atcctgccct catccattat tatgacccgg tccgccatgg ccgcttcttc
     3121 catgaaatgg gtgatcagga tcaccgttat accttcctga ttaagttccc ctattatatc
     3181 cattacttct ccgcggcctt ttggatccag catggctgtc ggttcatcaa aaattatgca
     3241 ttcgggcttc attgccacga ctcctgctat ggctatcctc tgtttctgcc cgcccgaaag
     3301 aagatggggc ggtttttttc tgtaatcccc catattgact gcactaagtg agtcttcaac
     3361 cctttttcgt atctccgaag gggatatccc cagattctcc ggaccaaaag ccacatcctc
     3421 ttcaactatg gatgaaacga tctggttgtc gggattttga aacaccatgc cggcggtttg
//
LOCUS       DAONHK010000115         3436 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_22075_length_3436_cov_2.820467, whole genome shotgun sequence.
ACCESSION   DAONHK010000115 DAONHK010000000
VERSION     DAONHK010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3436
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22075_length_3436_cov_2.820467"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1371)
                     /locus_tag="PLM92_04785"
     CDS             complement(<1..1371)
                     /locus_tag="PLM92_04785"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HPR24485.1"
                     /translation="MSFVSGQFLLFVAVVVVLFYVVPQKYKWVLLLVASYSFYLFGGI
                     KPVIFILLSTLSTFGAGLMMGRYEKKQKKEKRRILILLLLLNFGILLLLKYYNFFAEN
                     IDPLFRTFEYDISMPLVNIMLPLGISFYTFQAMGYAIDIYRGKYEPETNLAHFALFVS
                     FFPQIMQGPISRYNQLAPQFFAEHRFEYKKVKFGIQLMLWGFFKKMVIADRMAILVDT
                     VFPYYYGYDGDQVFVAMFCYAIQIYADFSGGIDITRGLAGAMGIELVQNFQRPYFAIT
                     VPEYWRRWHMSLTNWMRDYVFFPLTLSKMSNKLGRWSRKKFKGVVGRQIPSYLPTFIV
                     FFLIGVWHGASWGYIAFGFYNGTVIVLGMMLKPLFEAVNKKLGIKEGNTLFKIWQIIR
                     TFLIMMVGKTLVRAVSVKDGILMTLIAFDPFRWDIPHLPWNMVGMGLGKNQMLVLAIS
                     CIVFFFV"
     gene            complement(1390..1623)
                     /locus_tag="PLM92_04790"
     CDS             complement(1390..1623)
                     /locus_tag="PLM92_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HPR24486.1"
                     /translation="MEKIIEILNDIKPNVDYSNEKKLVDDEILDSFEVVAIIGELSEA
                     FGIEITVDEMIPENFNSAEAMEKLVKRLLEEQK"
     gene            complement(1692..2567)
                     /locus_tag="PLM92_04795"
     CDS             complement(1692..2567)
                     /locus_tag="PLM92_04795"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPR24487.1"
                     /translation="MNKQLMADMMLVFVTVCWGISYFLMDICLCDMGAFTLNMYRFIG
                     AFAVAFILGFPRLKKVSKTTIKYSFFLGIALCVTYIGATYGVMYTSLSNAGFLCALAV
                     IFTPILAFIFKGVVPEKKLAFVVIMCTIGIALLTLDENLSFASGDILCLICAFAYAVQ
                     MLITETAVRKEDVNAFHLGVFQLFFTGIFCGILAFLIEEPHLPSNVTVWMSVLFLMIF
                     CTGAAFIIQAIAQQYTSATRVGVIFTLEPVFAGFVAYFLAGEVLLPRAYAGAGLLIFS
                     LLLMEIDLKKIIKKP"
     gene            complement(2585..>3436)
                     /gene="holA"
                     /locus_tag="PLM92_04800"
     CDS             complement(2585..>3436)
                     /gene="holA"
                     /locus_tag="PLM92_04800"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HPR24488.1"
                     /translation="LPILSPKKLVVLKDYLPGENERVIEYFEDLPESTILVLTYEEGA
                     GKPKAAAGKKNDGEKETSNRRPGRDIIYKMVKSKGKIYDFGPLGRIQLSKFISKRLRG
                     LKKEADPSLISMIIAESGYYNRDGDYSLYNTENDVKKMADHCSGSELTGEDVLAGLSE
                     NLERNIFAMLDAVSGNRKDEAFRLLYDLLRSGESPPKLIAITASQLELMLCIKEMRQE
                     GKTRIQMEKEMNVHEYRLKKAMGFAEKYSERELRRILHSVYETDGRIKTGFLEPKMAL
                     EMMIAGI"
BASE COUNT          988 a          803 c          633 g         1012 t
ORIGIN      
        1 tacaaagaaa aatacgatac agcttatcgc aagtacaagc atctggttct taccaagacc
       61 catacccacc atattccagg gaagatgagg tatatcccac ctgaacgggt caaaggcgat
      121 cagggtcatc aaaataccgt cttttacgga aaccgccctt acaagggtct tccccaccat
      181 cattataagg aaagtcctta ttatctgcca tattttgaat aaagtgttcc cctctttgat
      241 accgagtttt ttgtttactg cctcaaaaag cggtttcaac atcattccca gaactatcac
      301 cgttccgtta taaaacccaa aggctatata accccagctg gctccatgcc atacacctat
      361 aagaaaaaat acgatgaatg tgggaagata tgacggtatc tgcctgccta caactccctt
      421 gaattttttt cttgaccatc tgcccagttt gtttgacatt tttgaaaggg tcaaagggaa
      481 gaaaacatag tctctcatcc agtttgtgag ggacatatgc catctgcgcc agtattcggg
      541 gacagttatg gcaaaataag gtctttggaa gttctgcacc agctctatac ccatggcacc
      601 ggccagtccc cttgttatat ctattcctcc ggaaaagtca gcatatatct gtattgcata
      661 gcaaaacatt gcaacaaaca cctgatcccc gtcatatccg taataatatg gaaaaaccgt
      721 gtccaccagg atcgccatac ggtcggctat gaccatcttt ttgaagaatc cccaaagcat
      781 tagctgaata ccgaatttta cttttttgta ttcaaacctg tgctctgcaa aaaactgggg
      841 agcaagctga ttgtatctgc ttataggtcc ctgcattatt tgaggaaaga aggaaacaaa
      901 gagtgcaaaa tgagccagat ttgtttcggg ctcatatttg cccctgtaga tatctattgc
      961 atatcccatt gcctgaaatg tgtaaaatga tatcccaagg ggcagcatta tattaacaag
     1021 aggcatggaa atatcatatt cgaatgtcct gaataacgga tcaatattct ctgcaaaaaa
     1081 attgtagtat ttaagaagca gcagtatacc gaaattcaga agcagaagca gtatgagtat
     1141 ccttcttttc tcctttttct gctttttttc gtatctgccc atcatgagcc cggcaccaaa
     1201 agtagaaagg gttgaaagaa gaataaaaat aacgggtttg atccctccga aaagatagaa
     1261 ggaataactc gctacaagaa gaagtaccca cttgtatttt tggggcacaa cataaaacaa
     1321 taccactact acggcaacga acagtaaaaa ctgccctgat acaaaagaca tcttccctcc
     1381 cgtcagtctt tatttctgtt cttccaaaag ccttttcacc aatttctcca tggcttctgc
     1441 ggaattgaaa ttctcgggaa tcatttcatc caccgttatc tctattccga aggcttcgga
     1501 aagttctcct attatggcaa caacttcaaa tgagtccagt atctcatcat caaccagttt
     1561 cttttcattt gaataatcca cattgggctt gatatcgttc agtatctcta ttattttctc
     1621 caagttatga tctcctttct ttttgtgttt taatccgact tttatattat acagacaagt
     1681 tgctctgatt tctatggttt ttttatgatt ttttttaaat ctatctccat caaaagaaga
     1741 ctgaatatca gtaagcccgc ccccgcataa gcccttggca aaagcacctc tccggccaga
     1801 aaataagcaa caaatccggc aaacaccggc tccagtgtga aaatcacacc cacacgggtt
     1861 gcactggtat actgctgagc aatagcctgt atgataaaag ctgccccggt gcagaatatc
     1921 atgagaaaca aaaccgacat ccatactgta acgtttgacg gcagatgagg ttcttctatc
     1981 agaaaggcaa gtattccgca gaatattcct gtgaaaaaaa gctgaaaaac cccgagatgg
     2041 aatgcattga catcttcttt tcttactgca gtctccgtga tcagcatctg taccgcatag
     2101 gcaaaggcac aaataaggca aagaatatct ccggaagcga aactgaggtt ttcatccagg
     2161 gtgagaagag ctatacctat ggtacacata attactacaa aagccagttt tttttcagga
     2221 acgacccctt tgaatatgaa agcaagtatg ggagtgaata taactgcaag ggcacaaagg
     2281 aaaccggcat tcgaaaggct ggtgtacatt accccgtatg tggctcctat gtaggtcaca
     2341 caaagggcta tacccagaaa aaagctgtat tttatggtgg ttttgctgac ttttttcagt
     2401 ctggggaatc caagtataaa tgcaaccgca aaagcgccta tgaatctgta catgttaagt
     2461 gtaaaagcgc ccatatcaca aagacaaata tccatgagaa aatatgagat gccccagcag
     2521 acagtaacaa aaaccagcat catatctgcc attaactgtt tgttcattct ttctcctaaa
     2581 gtttctatat cccggctatc atcatctcaa gagccatttt gggttccagg aatccggtct
     2641 tgatccttcc gtcggtttca taaacgctgt gcagtatcct tcttaattct ctttcggaat
     2701 atttttccgc aaatcccatg gcttttttca gtctgtactc atgcacattc atttccttct
     2761 ccatttggat gcgtgtcttt ccttcctggc gcatctcttt tatgcaaagc atcagttcca
     2821 gttgggatgc ggttatggca ataagcttag gcggggattc tcccgaacgg agcagatcat
     2881 aaagaagtct gaaggcctcg tcctttctgt tgccgcttac agcatcaagc atggcaaata
     2941 tattcctttc aagattttca gacaaccctg caagaacatc ttccccggtc aattcgctgc
     3001 ccgaacaatg atccgccatt tttttgacat cattttcggt attatacaga gaataatccc
     3061 catcccggtt atagtatccg ctttcagcta ttatcatcga tatcaaagac gggtccgcct
     3121 cttttttcag tcctcttaat cttttgctga taaacttgga caactggatc ctgccaaggg
     3181 gaccaaaatc atagatcttc cccttgcttt ttaccatttt gtagataata tccctgccgg
     3241 gtctgcggtt tgatgtttct ttttctccat catttttttt gccggcggca gcttttggtt
     3301 ttcctgcccc ctcttcatat gtgagtacca gtatcgtgct ttccggcaga tcctcaaaat
     3361 attctattac tctttcattc tccccgggca gatagtcttt taataccacc agctttttgg
     3421 gcgaaagtat gggcag
//
LOCUS       DAONHK010000116         3403 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_22401_length_3403_cov_3.980585, whole genome shotgun sequence.
ACCESSION   DAONHK010000116 DAONHK010000000
VERSION     DAONHK010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3403
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22401_length_3403_cov_3.980585"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(62..1174)
                     /locus_tag="PLM92_04805"
     CDS             complement(62..1174)
                     /locus_tag="PLM92_04805"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HPR24489.1"
                     /translation="MFLLVSALFFAVLFMPQKCLAASAPSSANIMDQMTLEEKIGQMF
                     LVCYDGSYDPAEEAADYYLGGYVFFADYFKGMNRKDVISGIKQIQKTAPLPLLTAVDE
                     EGGSVIRISGNPALHEKPFSSPSQVMERGGFFAAKDDASKKADMLLDLGINTNLAPVC
                     DVAQSNKDYIYERTIGEDPAKATAYAVSVVLASKEKNLGTCLKHFPGYGGSSDTHLGM
                     ATDNRPYKEFAKSDFMPFFFGIKAGADSVMVSHNIVNSMDPALPASLSKKVHDILRKD
                     FGFDKVIITDDISMGAITDLAGKQDTTAEGDPAEKNEAAVMAVKAGNDLICTRDFREQ
                     IPAVTDAVKKGVISEKQINRSVKRIIDWKISLGLIK"
     gene            complement(1259..2263)
                     /locus_tag="PLM92_04810"
     CDS             complement(1259..2263)
                     /locus_tag="PLM92_04810"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HPR24490.1"
                     /translation="MATKERVFKALKENKGKAVSGESLARSLNISRTAVWKAIKTLRN
                     EGYPIEAGTNKGYMLAAHLDYLSEDSIIAGLDGDLSDFSIHLYDTLESTNKTAKELAV
                     KDVDYNIAVIADHQTSGRGRLGREFFSPKGSGLYISLLLKPSFDMSKSVLITTAAAVA
                     AVRAIKKISDADLKIKWVNDIYMDDKKICGILTEAVTDFETGQIQHIVLGFGINCFEA
                     EYPEELSEKVSFIGGTFSRNMLAAEIINETMKIFNNIESRKFLEEYKNLSMVIDREID
                     VYKPAACASRTAVPIKALALGIDSDGGLIIRHEEDPFKGHIETLNSGEISIRVKKNKK
                     "
     gene            2458..3117
                     /locus_tag="PLM92_04815"
     CDS             2458..3117
                     /locus_tag="PLM92_04815"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HPR24491.1"
                     /translation="MMKNMSMKKISRIIIAFTLTAGLIFTMAPEATYAASISKKSVSH
                     VGKLIKVAKSKRGCRYSAGARGPNRFDCTGFVYYCMKKSGIKIRKGRLAPYKSSKYNV
                     GRSIKKAKKGDILLYYRGGRIGHAAIYIGGGKVIHATSRGVRITRWNGFGQRLAAVIR
                     VYSPAKKKKVTASKPKVTAVKTTPAPVTEPPPSTTEQTPPVTETPDAGTSVSPSTVPT
                     E"
     gene            complement(3190..>3403)
                     /locus_tag="PLM92_04820"
     CDS             complement(3190..>3403)
                     /locus_tag="PLM92_04820"
                     /inference="COORDINATES: protein motif:HMM:NF014669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015225 - biotin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015878 - biotin transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HPR24492.1"
                     /translation="HSFIWIVLSVTTGLFVCYLLGTLWFMISTATGLIPSLLMCVVPF
                     LPGDALKIIASSLLIKKLRPLVFKKL"
BASE COUNT          997 a          717 c          714 g          975 t
ORIGIN      
        1 cgctttctgg atctcttcaa atggatacat accatctcct cctaatgttt ctttttaatg
       61 gttattttat caaacccaga gaaattttcc aatcaataat tcttttgacg gagcggttga
      121 tctgtttttc acttattaca ccttttttga ccgcatccgt gacagccggt atctgttctc
      181 tgaaatctct tgtgcatata agatcattgc ccgctttaac ggccattact gccgcttcat
      241 ttttttcggc cggatcccct tctgcggtcg tatcttgttt gccggccaga tccgttatag
      301 ctcccatact tatatcatct gtgattatga ctttgtcaaa accgaaatct tttctgagta
      361 tatcatgaac tttttttgac agtgatgccg gaagggcagg atccatggaa ttgacaatgt
      421 tatgactgac cataacagaa tctgcccctg ctttgatgcc gaagaaaaaa ggcatgaaat
      481 cggatttggc aaattccttg tagggcctgt tgtcggtggc catgccaagg tgagtatcgc
      541 tgctacctcc gtaaccggga aaatgcttga gacatgtacc caggtttttt tctttggacg
      601 ctaatacaac cgaaactgca taagcagtgg cctttgccgg atcttcgccg atggtccttt
      661 catatatata atccttattg ctttgagcca catcacagac gggggcaaga ttagtgttta
      721 ttccaagatc aagcagcata tcggcttttt ttgaggcatc atcctttgcg gcgaaaaagc
      781 cgccccgttc catcacctgt gagggagaag aaaaaggttt ttcgtgtaag gccggattcc
      841 cgctgatacg gatgaccgat cctccctctt catctacagc agtcaaaaga ggaagaggtg
      901 cggttttctg gatttgtttg atcccggaaa taacatcttt tctgttcatt cctttgaaat
      961 agtcagcaaa aaaaacataa cctccgagat agtagtcggc ggcttcttct gcaggatcgt
     1021 aagagccgtc ataacagaca agaaacatct gtccgatttt ttcttcaagg gtcatttgat
     1081 ccattatgtt tgctgaagag ggagcggatg cggcaagaca tttttggggc ataaaaagta
     1141 cagcaaaaaa taatgctgaa accaaaagaa acatcgtgaa gattttcaaa tatttgtatg
     1201 attttgcttt ttccatggat aaatattaac acaatcagat aagaaaaggg agaccggttt
     1261 attttttgtt cttttttact cttatagaga tctcgccgct gtttagagtt tcgatatgac
     1321 ctttgaacgg atcttcttca tgtcttatga taagaccgcc gtcactgtct atacccaggg
     1381 caagagcttt tatgggaact gccgtcctgg aagcacaggc cgccggcttg tagacatcta
     1441 tttctctgtc aatgaccatg gaaagatttt tgtattcctc aaggaacttt ctgctttcga
     1501 tattattaaa aatcttcatg gtttcgttta ttatctctgc agccagcata ttccttgaaa
     1561 aagtgccgcc gataaaggag accttttcgg aaagttcttc gggatattcg gcctcaaaac
     1621 aatttatgcc gaagcccagg acgatatgct gtatctgccc ggtttcaaaa tcagtcacag
     1681 cttcagtaag gatgccgcat atttttttgt catccatgta tatgtcgttc acccatttta
     1741 ttttaagatc cgcatccgag atctttttta ttgcacgaac agcggcaacg gcagctgctg
     1801 ttgtgattag cacggatttg ctcatgtcaa aagaaggttt caaaagaaga cttatataaa
     1861 gccccgaacc ttttggagaa aagaattctc ttcccagcct tccccgtccg cttgtctggt
     1921 ggtcggctat tacagctata ttgtaatcaa catccttaac agccaattct tttgctgttt
     1981 tattagttga ttccagagtg tcatagagat gtatggaaaa gtcagacaga tccccatcaa
     2041 ggcctgcaat tatggaatcc tctgaaaggt agtcaagatg tgccgccagc atatatcctt
     2101 tatttgttcc ggcttctatg ggatatccct cattacgcaa agtttttatg gctttccata
     2161 cggcagtacg ggagatattc aaagatcttg ccaggctttc acctgatacg gctttcccct
     2221 tattttcttt gagtgctttg aaaacacgtt ctttggtggc cattcaagcc ctcctttttc
     2281 atcatatttt gtattgtaac atagcataaa aaaattgtaa acttgtatac attaaaacag
     2341 ttgacaataa gcaaagattt atgtgggtat tgtgaaaatg acaaatatgt cattgacctg
     2401 tcactttatg tcatttaagc taaacccatg atgtgaaaag gaaggcaact ttactttatg
     2461 atgaaaaata tgagcatgaa gaaaatcagc aggataataa tagcatttac tcttaccgca
     2521 ggacttattt ttacaatggc tcctgaggcc acctatgcgg caagcatttc aaagaagtca
     2581 gtttcccacg tgggaaaact cataaaagta gcaaaaagca aaagagggtg tcgctacagc
     2641 gccggagcca gaggaccaaa cagatttgac tgtacaggtt tcgtttatta ttgcatgaag
     2701 aaatcaggga tcaaaatcag aaaaggccgt ctggcacctt acaaaagcag taagtataat
     2761 gtaggcagaa gcataaaaaa agcaaaaaaa ggcgatattc tcctttatta cagaggcgga
     2821 aggatcggtc atgcagccat atacataggc ggaggcaaag tgatacatgc tacatcaaga
     2881 ggtgtgagga tcaccagatg gaacggattc ggccaaagac tggcagcggt aataagggta
     2941 tacagcccgg cgaagaagaa gaaagtcaca gccagcaaac ccaaggtaac agctgttaag
     3001 acaacaccgg ctcccgtaac agaaccgccg ccaagcacaa ctgaacagac accgccggtt
     3061 acagaaacac cggatgccgg tacatcagtt tcaccgtcaa cagttccgac agaataacaa
     3121 caataaaaac atctgaatgt gaatcagaga gaaagcgggg accctttatg ggtcctcttt
     3181 gttttttgtt taaagttttt tgaaaaccag aggccgcagt ttttttataa gcaaagaaga
     3241 agcgatgatt tttagtgcgt ctcccggaag aaaaggcact acacacataa gaagtgaagg
     3301 gatgaggcct gttgccgtgg aaatcatgaa ccacaaagtt cccaaaagat aacagacaaa
     3361 aagcccggtt gttaccgata aaacgatcca aataaaagag tgt
//
LOCUS       DAONHK010000117         3380 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_22606_length_3380_cov_4.562406, whole genome shotgun sequence.
ACCESSION   DAONHK010000117 DAONHK010000000
VERSION     DAONHK010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3380
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22606_length_3380_cov_4.562406"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            71..994
                     /locus_tag="PLM92_04825"
     CDS             71..994
                     /locus_tag="PLM92_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016294470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HPR24493.1"
                     /translation="MKEPVLIIMAAGMGSRYGGLKQIDPVTDRGEIIMDFSLYDAMMA
                     GFKKAIFVIKEEMKDDFYKLMENRSGKYLETEYAFQKLSDIPEGYKIPAGREKPWGTA
                     HAVLSCRDKIEGSFAVINADDYYGPGAFHKVYDFLSSEKEETDGDFCMVGYELSKTLT
                     ENGHVARGVCDISKNDYLRHIVERKKIMRVEDDICYSEDDGKTWMALPQDATVSMNFW
                     GYTLRMMEELEKGFPEFLKESINEDPLKGEYLLPSVTGKLIHEGKAAVKVLNSSDKWY
                     GVTYKEDKPGVMAALQSLKDKGVYPEILFSC"
     gene            1062..1502
                     /locus_tag="PLM92_04830"
     CDS             1062..1502
                     /locus_tag="PLM92_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPR24494.1"
                     /translation="MRKRRIFIIFLSVMVLVAGAFVLIKTAAPEKQKTVLASAQKKQE
                     QNPQKENKTKKDKETKIELTYGEKNKYTAEDEELYAIPEGEYEVTLSEASQEETGTVL
                     VEKDENTYQKVTQYVFSEGESQKVVVNEGEQVSVSQGTSWEFDK"
     gene            1502..2188
                     /locus_tag="PLM92_04835"
     CDS             1502..2188
                     /locus_tag="PLM92_04835"
                     /inference="COORDINATES: protein motif:HMM:NF025736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HPR24495.1"
                     /translation="MKPEKIRLKYCIIPLAFAELQNALTFMGTMWLRSDTAGITNITT
                     DFDRMIPVIPQFVYVYLGFFAFWIISFVLAARTGPKFFYRMIAAYILADAVCLLFYIF
                     FPTTNIGDRAEIEGDSLSWLLLSFVYFASKPTNLFPSTHCFVTWLSYICVRGQKHINK
                     VLRISFLVIAVLILLSTQFTKQHVFIDIPAGMLLAEGMYILCGRWQKFTGLIEKVFTC
                     INKKVFKDLR"
     gene            2302..2589
                     /gene="rpsF"
                     /locus_tag="PLM92_04840"
     CDS             2302..2589
                     /gene="rpsF"
                     /locus_tag="PLM92_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012104223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HPR24496.1"
                     /translation="MTNYEVLFILDPILEDEKKEATVETVKGIIETEGTVGKVDVWGM
                     RKLAYPIRKKEDGYYVLIEFSANETLPKELDRRMKISDDIMRHLIVNKDEK"
     gene            2601..3011
                     /locus_tag="PLM92_04845"
     CDS             2601..3011
                     /locus_tag="PLM92_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005999075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HPR24497.1"
                     /translation="MNNVTLIGRLTKDPEVRYTTDQMAIAKFTVAVDRFGKEKQADFI
                     PVTVFGKQAENCEKYLVKGRKVAIQGRIQTGSYENKEGKRVYTTDVVANTVEFVEWGD
                     RDGSPSRTGENSVAKAKDGVPEGFQSIDDDDIPF"
     gene            3035..3271
                     /gene="rpsR"
                     /locus_tag="PLM92_04850"
     CDS             3035..3271
                     /gene="rpsR"
                     /locus_tag="PLM92_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HPR24498.1"
                     /translation="MEKKRRSGGFRKKKVCQFCADKSQTIDYKDVDTLRRYVTERGKI
                     LPKRITGTCAIHQREVTKAIKRARAVALLPYTAD"
BASE COUNT         1162 a          575 c          814 g          829 t
ORIGIN      
        1 aggcaatgta tcacgtaaag agacatggca aaaaaggata ttccttcaca aaaggaaagg
       61 agtgatcgga atgaaagaac cggttttgat aataatggcc gcagggatgg gatcccggta
      121 cggcgggctc aaacagatag acccggtaac agaccgggga gagataataa tggatttttc
      181 tctttatgat gcaatgatgg caggattcaa aaaagccatt tttgtcataa aagaagaaat
      241 gaaagatgat ttttataaac tcatggaaaa cagatcggga aaatatcttg aaacggaata
      301 tgcttttcaa aaactttcgg atatacccga aggatataaa atacccgccg gaagagaaaa
      361 accctggggt acggcacacg cagtgctgag ctgcagagat aaaatcgaag gatccttcgc
      421 tgtcataaat gccgatgact attacggtcc cggggcattt cacaaggtat atgattttct
      481 ttcttcagaa aaagaggaga ctgacgggga cttttgtatg gtcggatacg aactaagcaa
      541 aactcttact gaaaacggtc atgtggccag gggtgtatgc gatatatcaa aaaacgatta
      601 tctcagacac atagttgaga gaaaaaaaat aatgagagtg gaagacgata tatgttattc
      661 cgaagacgac ggaaagacat ggatggcact tccccaggat gcaactgtat caatgaactt
      721 ctgggggtac accttaagaa tgatggaaga acttgaaaag ggttttcctg aatttctcaa
      781 agagagcatc aatgaagacc ccttaaaagg cgagtacctt ctcccatctg ttaccggaaa
      841 gctgatccat gaaggaaaag cggcggtgaa ggtgcttaat tcctctgaca aatggtatgg
      901 agtcacatat aaggaggata agcccggggt tatggcggca ttgcagtcat taaaagataa
      961 gggggtttat ccggagatac tgttttcttg ttaaaaagca aaaaaggcgg tataatatat
     1021 aagagtttgt tttgtccgtt attttgcgga ggtatgggac tttgagaaaa agaagaatat
     1081 ttatcatatt tctgtcggtg atggttttgg ttgccggagc gtttgttttg ataaaaacag
     1141 cagcaccgga aaaacagaaa acagttttag cgtccgctca aaaaaagcag gaacaaaatc
     1201 cccaaaaaga aaacaaaaca aaaaaagata aagaaacaaa gatagaactt acatacggtg
     1261 aaaaaaataa atacacagcg gaagatgaag agctttatgc cattcccgaa ggggaatatg
     1321 aagtgaccct atcagaagca tcccaagagg aaacgggaac ggtgcttgtt gaaaaagacg
     1381 aaaacactta tcagaaagtg actcagtatg ttttttcgga gggagaaagc cagaaggtgg
     1441 tagtaaatga aggagagcag gtttccgtaa gtcagggaac cagctgggaa tttgataaat
     1501 aatgaaaccg gaaaagatcc gcctgaagta ttgcattata cctttggctt ttgcagagct
     1561 tcaaaatgca ttgacattca tgggcaccat gtggctgagg agcgatacgg ccgggataac
     1621 aaatataacg actgactttg acaggatgat tcctgtcata cctcagtttg tttatgtata
     1681 tcttggtttt tttgcttttt ggataatcag ttttgtgctg gcggcaagga ccgggccgaa
     1741 atttttttac aggatgatcg ccgcatatat tcttgccgat gccgtgtgcc ttttgtttta
     1801 tattttcttt cctaccacaa atatcggaga cagggcagaa atagagggag attccctttc
     1861 atggcttttg ctttcttttg tttattttgc atcaaaaccc acaaatcttt ttccatcgac
     1921 ccactgtttt gtaacatggc tttcatacat ttgcgtcaga gggcaaaaac acataaacaa
     1981 agtcctgagg atttcttttc ttgtcattgc cgtccttata ttactttcaa cacagtttac
     2041 gaagcaacat gtttttattg acatacctgc aggcatgctg ctggcggagg gaatgtatat
     2101 cctgtgcggc agatggcaaa aattcacggg tcttattgaa aaagtattta catgtataaa
     2161 caaaaaggtt tttaaggatc tgcgataaaa gccttgcaat ttgccgcatt taaggtataa
     2221 tacataagca tgtaaattta taggtgcata ccctgcgtca gcatgatgct gacgccatta
     2281 agtccacagg gaggtgaaaa aatgacaaat tatgaagttc tgttcatcct ggatccaatt
     2341 ttggaagatg agaaaaagga agcgacggta gaaaccgtaa aaggcatcat tgagacagaa
     2401 ggtaccgttg gcaaggtcga tgtctgggga atgagaaaac tggcctatcc catacgtaaa
     2461 aaggaagacg gctattatgt gctcattgag ttcagtgcaa atgaaactct gccaaaagaa
     2521 cttgaccgca gaatgaagat atctgatgat atcatgagac atttgatagt caataaagat
     2581 gaaaagtaag gagaccggtt atgaataatg taacattgat cggaagactg acaaaggatc
     2641 cggaagtaag atatacgaca gaccagatgg ccatagcaaa gtttactgtt gcagtggata
     2701 gattcggaaa agaaaaacag gcggatttca ttcccgtgac cgtatttggc aaacaggcgg
     2761 agaactgtga aaaatattta gtgaagggtc gtaaggtggc catacaggga cggatacaga
     2821 caggaagcta cgaaaacaaa gaaggaaaaa gagtttacac aacggatgtg gtggcaaaca
     2881 cagttgagtt cgtagaatgg ggagacagag acggatctcc gtcaaggacc ggagaaaaca
     2941 gcgtggccaa ggcaaaagac ggtgttcccg aaggtttcca atctatagac gatgatgaca
     3001 tacccttcta aaaaggtgaa aggagcaaag aaccatggaa aagaaaagac gcagcggcgg
     3061 cttcagaaaa aagaaggttt gtcagttttg cgctgacaaa tcacagacca tagattataa
     3121 agatgttgac acacttagaa gatatgtgac cgaaagaggc aaaatactgc caaagagaat
     3181 aacaggaact tgtgcaatac atcaaagaga ggtcacgaaa gccataaaaa gagcaagagc
     3241 tgtagcactt ttgccctata ctgcagatta agaaaaagac ttgacaagag cgggtatccg
     3301 ctcttgtttt tttgaaggac cactgacaca acatgtagta gaggacaaaa gaagcacttt
     3361 atgttataat attttggcat
//
LOCUS       DAONHK010000118         3363 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_22769_length_3363_cov_4.114873, whole genome shotgun sequence.
ACCESSION   DAONHK010000118 DAONHK010000000
VERSION     DAONHK010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3363
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22769_length_3363_cov_4.114873"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1345)
                     /locus_tag="PLM92_04855"
     CDS             complement(<1..1345)
                     /locus_tag="PLM92_04855"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HPR24499.1"
                     /translation="MKNSSSNKFSQWELFPILDSISDAVFIDDSNGYALWCNKACMDL
                     YKVAPKDVVGVHVLDLEKNGVFSPSVANLVLEQKKEVTIIHENKEGKKLLSTGTPIKD
                     ENGNITKVITTSRDITELSTLQNKLQTVQGTLEEMKSFEKLSGQGIIASSHSMFNVVQ
                     LAKRLSAVDSTVLITGESGVGKGLIAEILHKNGNRKDAPFVTVNCGAIPENLIESELF
                     GYESGAFTGSRSEGKKGLFEAAQSGTIFLDEISELPLNLQVKLLQVIQEREITKVGGV
                     KKIPVDVRIISATNKDLHELVSENRFREDLYYRLNVVPINVPPLRERPEDILPLINKY
                     LEKYNEKLNEHKQIDQNALAVLLKYDWPGNIRELQNITERLILTCREDIITADELPGF
                     ITETINEPGEEFPSGPMSLAGSMANAEKKILSASLNKYKSTRAIAKALGISQPTVV"
     gene            1465..1845
                     /locus_tag="PLM92_04860"
     CDS             1465..1845
                     /locus_tag="PLM92_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008398832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin family protein"
                     /protein_id="HPR24500.1"
                     /translation="MMKKFYVCRHCGNIVLKVKDAGVPLVCCGENMTELIPGTVDAAL
                     EKHVPKVTVSGSTITALIGEVTHPMQEEHFIEWIYLETEKGAQIKYLKPGEEPKAVFE
                     VADGDKALAVYEHCNLHGLWKSEV"
     misc_feature    1944..2061
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            2105..2620
                     /gene="infC"
                     /locus_tag="PLM92_04865"
     CDS             2105..2620
                     /gene="infC"
                     /locus_tag="PLM92_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008901813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HPR24501.1"
                     /translation="MSRENLINEEIRDGELRIISQDGEQLGIMSRDEALRLAEEKGLD
                     LVNISPSAKPPVCKLLDYGKYRYELKKKEKEARKKQTIVQIKEIRLSTFIEDHDIMVK
                     AKTAYKFLENGDKVKVTLRFRGRERDYTDKGREVMEKFAEAVSEVSDIEKKPKFEGRS
                     LTMFLTPKAEK"
     gene            2663..2863
                     /gene="rpmI"
                     /locus_tag="PLM92_04870"
     CDS             2663..2863
                     /gene="rpmI"
                     /locus_tag="PLM92_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009644715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HPR24502.1"
                     /translation="MAKNKMKSHRGACKRFKLTGSGKVKRNKAYKRHILTKKSPKRKR
                     GLRKATIMTGADMKRIKNAMGK"
     gene            2882..3238
                     /gene="rplT"
                     /locus_tag="PLM92_04875"
     CDS             2882..3238
                     /gene="rplT"
                     /locus_tag="PLM92_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008677824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HPR24503.1"
                     /translation="MARVKKGVNAHKRHKKILKQAKGFYGQKSKVFKAANPAVMRALR
                     SAYVGRKNKKREYRKLWITRINAGARMNGLSYSRLMNGLRTSGIEINRKMLAEMAVHD
                     EAAFAELCDTAKKAVK"
BASE COUNT         1068 a          613 c          778 g          904 t
ORIGIN      
        1 gaactactgt gggctgactt attccaaggg ctttggcaat ggctctcgtt gatttgtatt
       61 tatttaatga agcggaaagg atcttttttt cggcatttgc cattgaaccg gccagggaca
      121 ttggaccgga agggaattct tcaccgggct catttattgt ttctgttata aaacccggaa
      181 gctcatctgc tgtgattata tcttctctgc aggtgaggat gagtctttct gttatattct
      241 gcagttctct gatatttccc ggccagtcat atttcaatag aacggctaag gcattctgat
      301 ctatctgttt atgctcattg agcttttcgt tgtatttctc caggtatttg tttataagag
      361 gtagtatatc ctcaggccgc tctcttaaag gaggcacatt tatggggacc acgttcaagc
      421 ggtaataaag gtcttcacgg aatctgtttt cactgaccag ctcatggaga tccttgttgg
      481 tggcggatat tatccgcaca tccaccggta tttttttgac tccgcccact tttgtgatct
      541 ctctttcctg tatgacctgg agcagtttga cctgaaggtt cagaggcagt tccgatattt
      601 catccaaaaa gatggttccg ctctgggcag cttcaaaaag tccctttttc ccttcggatc
      661 ttgaacctgt gaaagcgccg ctttcatagc cgaaaagttc tgattcgatg aggttttcag
      721 gtatagctcc gcagttcact gtaacgaagg gagcatcttt cctgtttccg tttttgtgaa
      781 gtatctcggc tatcaggccc ttgccgactc ccgattctcc cgttataaga acggtagaat
      841 ctactgctga aagtcgtttc gccagctgga ccacattgaa cattgagtgg ctgctggcta
      901 tgatgccctg gccggaaagt ttttcaaagg acttcatttc ttccagagtg ccctgaacgg
      961 tctgcagttt gttctgaaga gtggaaagtt ctgttatgtc cctggaagtc gttattactt
     1021 ttgtgatatt tccgttctca tcttttatgg gagttccggt ggacagcagt tttttgccct
     1081 ctttattttc atgtattata gtgacttctt ttttttgttc aagaacaaga ttggcaacag
     1141 aaggcgaaaa aaccccgttc ttttccagat caagaacatg gactcccaca acatccttgg
     1201 gagcaacctt gtaaagatcc atgcaggcct tgttgcacca aagagcatat ccattgctgt
     1261 catcaataaa aaccgcatcg ctgatgctgt ccagtatggg gaagagttcc cattgtgaaa
     1321 atttatttga agaagagttt ttcatttttc cctcactagt ttgatgtttt aattttggtg
     1381 tatatattat ataataacaa aaaacataat acaaggtatc cgatacaaaa tcgtatcgga
     1441 tcacggagaa ttgcggaggt aataataatg aagaaatttt atgtttgcag acattgcgga
     1501 aatatcgtat taaaagtaaa ggatgccggc gttcctctgg tttgttgcgg tgagaacatg
     1561 accgaactga ttcccggaac tgtagatgca gctttggaaa aacacgttcc aaaagtaact
     1621 gtttcgggaa gcacgatcac tgctttgata ggcgaggtaa cacacccgat gcaggaagag
     1681 cattttatag aatggatcta tctggagaca gaaaaaggcg cacagataaa atatctgaag
     1741 cccggggaag aaccgaaagc tgtttttgaa gttgctgacg gagataaggc tttggctgta
     1801 tatgagcact gcaatcttca cggcctttgg aaatctgaag tataaaggac attctgcgat
     1861 cggagaaaaa tataaatact atacaatgct caagtcagaa taagtacttg acaagttctt
     1921 tgagcaagtg gtatattaag cgggtaacta agaagaagtt atccgcttct cacctttttg
     1981 gccagtgtgc tggatcggtt gataaaaaag aattgcattt tgcatttaag cggatatttc
     2041 tgtccgcttt ttttatgccg ttatcctgcc gttttgggca gggcgaaatt ggaggtaaag
     2101 aacgattagc agggaaaatc ttataaacga ggaaatccgc gacggagagc ttagaataat
     2161 aagccaggac ggagaacagc ttgggataat gagccgtgac gaagcattaa ggctggcaga
     2221 ggaaaaagga cttgatctgg taaatatatc tccctctgca aaaccacctg tatgcaagct
     2281 tctggattat ggaaagtacc gttatgaact gaagaaaaaa gaaaaagaag ccagaaaaaa
     2341 gcaaaccatt gtgcagataa aagaaatacg tctcagtact tttatcgaag accatgacat
     2401 aatggtaaaa gctaaaaccg catataaatt ccttgaaaac ggagataaag taaaggttac
     2461 attgagattc cggggaaggg aaagagatta cactgacaaa gggcgcgaag tcatggagaa
     2521 atttgcagag gccgtttcgg aagtcagtga catcgaaaag aagcccaaat tcgaaggcag
     2581 gagtcttact atgtttctga caccaaaagc ggaaaagtga aaataacagt aaaaaaataa
     2641 agatacagga ggaattatca ccatggcgaa aaacaaaatg aaatcacaca gaggggcatg
     2701 caaaagattc aaactgacag gttccggaaa agtcaagaga aacaaggcat ataaacgcca
     2761 tattcttacc aaaaaaagcc caaaaagaaa aagagggctg agaaaagcaa ctataatgac
     2821 cggtgccgat atgaaacgta taaagaatgc gatgggcaaa tagaaagaga ggtaaggaaa
     2881 aatggcaaga gtaaaaaaag gcgtgaatgc gcataaaaga cataaaaaaa tactgaagca
     2941 ggcaaagggt ttttacggac agaaaagcaa ggttttcaaa gctgcaaatc cggcagtgat
     3001 gagagcactg cggtctgctt atgtgggaag gaaaaacaaa aaaagagagt acagaaaact
     3061 ctggataaca agaataaatg ccggtgccag gatgaacggt ttatcataca gcagattaat
     3121 gaatggcctg agaacatccg gtatagaaat aaacagaaaa atgctggcgg aaatggcagt
     3181 gcatgatgaa gctgcttttg cagaactttg cgataccgca aagaaagcgg taaaataaaa
     3241 agtcaaaaag caggacaatg tcctgctttt tatgaagaac agatgaataa atacttgcat
     3301 tatgttgaga aaatgaaaaa cccgtgttgt ttttcaacgc agctttaatt ttcaagtgat
     3361 gca
//
LOCUS       DAONHK010000119         3338 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_23012_length_3338_cov_3.126409, whole genome shotgun sequence.
ACCESSION   DAONHK010000119 DAONHK010000000
VERSION     DAONHK010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3338
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23012_length_3338_cov_3.126409"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1270
                     /locus_tag="PLM92_04880"
     CDS             <1..1270
                     /locus_tag="PLM92_04880"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HPR24504.1"
                     /translation="ISALFADIGDEQSIEQSLSTFSSHMKNIVEITKKAGAGSLVLLD
                     ELGAGTDPTEGAALAIALLEYLRAKNANILATTHYTELKKYALSAEGVENASMEFDVK
                     TLSPTFKLTMGIPGKSNAFEISKKLGLKDSITEKARELLSRGDIEFEEVISAIEEDKK
                     AAEKERDQAIALNIQMKKQKEEMDEEQRRLEERKEKIINKAKEEARDMIAEAKQICDE
                     VREELRNISKKESLSEKEQRNENSRRKIKDAAGRYRQQIIKEINDDPVRIEDVRTGDR
                     VKVLSLGQNGEIISLPDEKGEMTVQVGRLKVKAKTEDLKIINEGKKKKSSGAERHYRQ
                     LYSIKAKSVSVSINVQGQNLDEAKENVDKYLDDAFMAHLNEVTIIHGKGEGILKKGIQ
                     EMLKNHRHVSEYRKGGYNEGGDGVTVIKLK"
     gene            1355..1885
                     /gene="thpR"
                     /locus_tag="PLM92_04885"
     CDS             1355..1885
                     /gene="thpR"
                     /locus_tag="PLM92_04885"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HPR24505.1"
                     /translation="MRTFIAVTFDEGIKKDIEEIIIRIKRVSERGRFSPGRNLHLTLF
                     FLGDTDIKKIDDIKNAMDMVKCEKFDLKLLSPGRFSKGSSDIYWLGTEESIPLLRLQN
                     DLSRELMKIGFSAESKRFTPHLTIARQVKGDFKKACEAAGVKKTMIQKVDGISLMESR
                     RINGKQEYTEMYRKNF"
     gene            1912..2559
                     /locus_tag="PLM92_04890"
     CDS             1912..2559
                     /locus_tag="PLM92_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009003529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1847 domain-containing protein"
                     /protein_id="HPR24506.1"
                     /translation="MGMENTKSCVDCGTMKCSTKEGEYPDFCPARNFTEKEKRELISL
                     YGDDKENRMLSLAAAEVEFENYCRLTRVEETMEFAKKINAKKLGVATCVGLINESRIL
                     AKILRANGFEVMVVVCKTGSIQKKETGFMKTSKEIGDTMCNPLLQAKLLNEAKTDFNI
                     VMGLCVGHDSLFYKYSEAPVTTLVAKDRVTGHNPAAPLYNAGSYYKKLLKKGEHK"
     gene            2556..>3338
                     /gene="argS"
                     /locus_tag="PLM92_04895"
     CDS             2556..>3338
                     /gene="argS"
                     /locus_tag="PLM92_04895"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005999689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HPR24507.1"
                     /translation="MIDYKDIIAELITENTQGLSKDQVKVMIEVPADEKMGDYAFPCF
                     KLAKTLKKAPPLIAKDIAAKIEKHLLFDKVEQVNAYVNMFISREDFVSSIIKDAVSKE
                     ENYGRSTIGEGKKVIVEYSSPNIAKPFHIGHIRSTVIGNSIYKIYDFLGYDTVRINHL
                     GDYGTQFGKMICAYRHWGNKDDVVKEPIKTLLGYYTRFYKEAENDPGLDQEAREIFTK
                     LEHGEKEEVELWQWFRDESLKEFKRVYEMLGIEFDSYAGESFY"
BASE COUNT         1242 a          573 c          826 g          697 t
ORIGIN      
        1 gatatccgcc ctgttcgccg atatagggga cgagcagagc atagaacaga gcctttcaac
       61 attttcatcc catatgaaaa atatagtaga aataacaaaa aaggcaggag caggatctct
      121 ggtcctgctt gatgaactgg gtgcagggac ggaccccact gaaggagcgg ccctagccat
      181 agcactgctg gaatacctga gggcaaaaaa tgccaatata ctggcaacga cacattatac
      241 tgaactgaaa aaatatgcgc tttccgcgga gggcgtggaa aatgcctcaa tggaattcga
      301 tgtgaagact ttgagcccga ctttcaaact taccatgggt ataccgggta agtcgaatgc
      361 atttgaaata tcaaaaaaac tcggtctgaa agacagcata accgaaaagg ccagggaact
      421 tctttccaga ggtgatatag aattcgaaga agtgatttcc gccatagaag aagacaaaaa
      481 agcggcagaa aaggagaggg atcaggccat tgctctgaat atccaaatga aaaagcagaa
      541 ggaagaaatg gatgaggaac agagaagact tgaagaaaga aaagaaaaaa tcataaacaa
      601 agccaaggaa gaagctcgcg atatgatcgc agaagcaaag cagatatgcg atgaggtcag
      661 agaagaactc aggaacatat caaaaaaaga aagcctttcc gaaaaagagc aaagaaatga
      721 aaattccaga agaaagatca aagatgcggc cggcagatac agacagcaga taataaaaga
      781 aataaacgat gatcctgtga gaatagaaga tgtaaggacg ggagacaggg taaaggtgct
      841 cagcctgggt caaaacggcg aaataatatc tcttcccgat gaaaaagggg aaatgactgt
      901 tcaggtgggc aggctcaaag tgaaagcaaa gacggaagac ctgaaaataa taaacgaagg
      961 gaaaaagaaa aaaagcagcg gggcagagag acactacagg cagctttaca gtataaaggc
     1021 aaaaagtgtt tcagtgagca ttaacgttca gggccagaat ctggatgaag caaaggaaaa
     1081 tgttgacaaa tatctggatg acgcattcat ggcgcatctt aacgaggtta cgataataca
     1141 cggcaaagga gagggtatcc tgaaaaaagg aatacaggaa atgctcaaaa accacagaca
     1201 tgtttcggaa taccggaaag gtggctacaa cgagggtgga gacggagtca ctgttataaa
     1261 gctgaaatag atcgacaggg agaccggttc atcaactatc ctatccaccg attggcggat
     1321 tgcctgtaat tattctgaca tatgaggaat tattatgaga acttttatag cagtgacctt
     1381 tgatgaagga ataaaaaaag atattgagga aataataata aggataaaga gggtctctga
     1441 aagaggaagg ttttcacccg gaagaaatct tcacctgaca ctgttttttt tgggagatac
     1501 agacataaaa aaaatagatg acataaaaaa cgccatggat atggtgaaat gcgaaaaatt
     1561 cgatctgaaa ctgttatcac ccggaagatt cagcaagggc agcagtgata tatactggtt
     1621 gggaacagaa gaatcgatac cgcttctgag acttcaaaat gaccttagcc gggaacttat
     1681 gaaaatagga ttttctgcag aatcaaagag attcacacct catctgacaa ttgcaagaca
     1741 ggtcaaaggt gatttcaaaa aagcatgtga agcagcggga gtaaaaaaga ccatgataca
     1801 gaaagtggac gggataagcc ttatggaaag cagaaggata aacggaaagc aggagtatac
     1861 ggagatgtac aggaaaaact tctgaaaaat aaaggaggat gtgctataat tatgggcatg
     1921 gaaaatacaa aaagttgtgt agactgcggt accatgaaat gcagtacaaa agaaggcgaa
     1981 tacccggatt tttgccccgc aaggaacttt accgaaaagg aaaaaagaga gttgataagc
     2041 ctttacgggg acgataaaga aaacaggatg ctgtccctgg cggcggcgga agttgaattt
     2101 gaaaactact gcaggctcac acgagtggaa gaaaccatgg agtttgcaaa gaaaataaat
     2161 gctaaaaagc ttggtgtggc cacttgtgtg gggcttataa atgagagcag gatactggca
     2221 aagatattaa gggcaaacgg atttgaagtc atggttgtgg tctgcaaaac aggaagcata
     2281 caaaaaaaag aaacgggatt catgaagact tccaaagaaa taggcgatac tatgtgcaat
     2341 cctttgcttc aggcaaaact gcttaatgaa gcaaaaacag acttcaatat agtcatgggc
     2401 ctttgcgtag ggcatgacag ccttttttac aaatattcag aagcaccggt taccaccctt
     2461 gtggcaaaag acagggtcac cgggcataat ccggctgctc ccctttacaa tgccggctca
     2521 tattacaaaa aactgctgaa aaaaggagaa cataaatgat cgactacaaa gatattattg
     2581 cagaacttat taccgaaaat acccaggggc tttcaaaaga tcaggtaaaa gtcatgatcg
     2641 aggttcccgc cgatgaaaaa atgggagact atgctttccc gtgctttaag ctggcaaaga
     2701 ccttgaaaaa ggcacctcct ttgatagcaa aggatatcgc cgcgaaaata gagaaacatt
     2761 tattatttga taaagtggaa caggtgaacg catatgtcaa tatgttcatt tcacgcgagg
     2821 actttgtttc atctataata aaggatgcag tctcgaaaga agaaaactac ggcaggagta
     2881 cgatagggga aggcaaaaaa gtcatagtgg aatattcttc gcccaatatc gcgaaacctt
     2941 tccatatagg tcatatcagg agtaccgtga taggtaactc catttataag atatatgatt
     3001 ttctcggata tgatacggtg aggatcaatc atctgggaga ctacggcact cagttcggaa
     3061 agatgatctg tgcatacagg cactggggaa acaaagatga tgtggtaaaa gagccgataa
     3121 agaccctgct cggatattac accaggtttt ataaagaagc agaaaatgat ccgggccttg
     3181 atcaggaagc aagggagata ttcacaaaac tcgagcatgg tgaaaaagaa gaagttgagc
     3241 tctggcagtg gttcagagac gaatctctca aggaattcaa acgtgtatat gaaatgctgg
     3301 gcatagaatt cgattcctat gcgggagaaa gtttctat
//
LOCUS       DAONHK010000120         3258 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_23888_length_3258_cov_4.280674, whole genome shotgun sequence.
ACCESSION   DAONHK010000120 DAONHK010000000
VERSION     DAONHK010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3258
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23888_length_3258_cov_4.280674"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1116
                     /gene="argS"
                     /locus_tag="PLM92_04900"
     CDS             <1..1116
                     /gene="argS"
                     /locus_tag="PLM92_04900"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HPR24508.1"
                     /translation="EADKDPSLDQEAREIFTKLEHGQKEEVELWQWFRDESLKEFKRV
                     YEMLGIEFDSYAGESFYSDKMPRFVRELTEKGLLKKSEGAQIVDLEEYGMSPALITKS
                     DGSTLYITRDIAAAVYRKEHYDFYRNIYVVSSGQNLHFQQWIKIVELLGYEWARDCVH
                     IPFGLVSLEDGTMSTREGRVVFLEDVLSRAVEKTREIIKEKNVNTDDIEKTAKQVGIG
                     AVVFQELSNNRIKDYVFSWDRVLNFDGETGPYVQYTHARAASVLRKAGAAASVDAGKK
                     EIKAEYIKSDSAYELAKLIYTLPETVAEAGEKYEPSIITRHIINVAQAFNRFYHDEHI
                     LTEDGNERTAKLALVKAAKTTIKNGLGLLGIEAPEKM"
     gene            1117..1989
                     /locus_tag="PLM92_04905"
     CDS             1117..1989
                     /locus_tag="PLM92_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003485627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPR24509.1"
                     /translation="MRYEGNIYRPPSEARSLLIQVTIGCAHNECTFCSMFKDKKFRVR
                     PMEEIMEDLEDARRTYNFVEKIFLCDGDALCLSFDKLMTILRKIKDIFPECKRVTVYG
                     SPQDVLRKTEEELKGLYEAGVEIIYIGAESGSDKVLKNVKKGADRKEIIESVKKIEEA
                     KIKASVTFISGLGGRELWREHAKESGAMISEMQPSYVGLLTLMTEPEAPLTDDIAKGG
                     FLLLTAEEVVAETLLMLKNINVKKECIFRSNHASNYVSLRGTLPRDKDMMIKQLENAV
                     KNKGMLKDERFRML"
     gene            2006..>3258
                     /locus_tag="PLM92_04910"
     CDS             2006..>3258
                     /locus_tag="PLM92_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPR24510.1"
                     /translation="MSNKAKEKQNGGIKAPGEQKLTMAQSLGITKTVKRTEEETDIRK
                     RPDRISKYFRKYLNKFVFVEFSDEYLNGSKIKDIMKGVPVPLRRRDVKEFAGGKGLKA
                     THIAENMAWIMGCDPKFKYNQAYIDYMFKLFNYKIYEGMMKEGRDAAEIEDYDNACIH
                     FRASLCMKPDYLHGMYSYARVCRAMYMNSGNTEYVGRFKAEALDYFELVTETHPRFAQ
                     GYYYLGYAYLNIGLYSKAYAAWKWFLKFTRSGKDRKEINKRIAQIENPMRIEEGYNEI
                     LAGRYDQGVDILENYLDSQYKDWWPLHYYLGIGYARLGKKGDAFARFKHTLSLNGSHL
                     ETMEELVAIYEAEGDKENTNKYRKKIELIKADIAKEQEAIKKAAEKESGTEIVYLEQK
                     TKNKKEKSKTSSKETKKEEPEKAKE"
BASE COUNT         1189 a          583 c          812 g          674 t
ORIGIN      
        1 gaagcagaca aggatccgag ccttgatcag gaagcaaggg agatattcac aaaactcgag
       61 catgggcaaa aggaagaagt tgagctttgg cagtggttca gagacgaatc cctcaaagag
      121 ttcaaacggg tatatgaaat gctgggcata gaattcgatt cctatgcggg agaaagtttc
      181 tattcggaca aaatgcccag gttcgtcagg gaacttacgg agaagggact gctgaaaaaa
      241 tcagaaggag cacagatagt ggaccttgaa gaatacggca tgtcaccggc actgataacc
      301 aagtccgacg gctctaccct ttatatcacc agggacattg cagcggcagt atacagaaaa
      361 gagcattatg atttttacag gaatatatat gtagtctcat caggacagaa ccttcatttc
      421 cagcagtgga taaaaatagt ggaactgctg ggatacgagt gggccaggga ctgcgtccac
      481 ataccatttg gtcttgtaag tctcgaagac ggcaccatgt ccacaagaga aggccgggtg
      541 gtattcctgg aggatgtgct ttcaagagca gtggaaaaaa caagggaaat aataaaagaa
      601 aagaatgtta atacggacga tatagaaaaa accgcaaaac aggtgggaat aggagcagta
      661 gtgtttcagg aactttccaa taacaggata aaggactatg ttttttcctg ggacagagtc
      721 cttaattttg acggcgaaac aggaccttat gtgcagtaca cccatgccag agctgccagt
      781 gtcttgagaa aagcaggagc agcagcttca gtagatgccg ggaaaaaaga aataaaagcc
      841 gaatacataa aaagcgacag cgcctatgag ctggcaaagc tgatatatac attacccgaa
      901 acagtagccg aagcaggaga gaagtatgag ccttcgataa taacaagaca cataataaac
      961 gtggctcagg ctttcaacag gttttatcat gatgagcata tactgacaga agacgggaat
     1021 gaaaggacag ccaagcttgc cctggtaaaa gcagccaaaa caaccatcaa aaatggtctt
     1081 ggacttttgg gcatagaagc accggagaag atgtaaatga gatacgaagg gaatatatac
     1141 agacctccca gcgaggcaag gagtcttctg atacaggtaa cgataggttg tgcccataat
     1201 gagtgtacct tctgcagcat gttcaaggac aagaaattca gagtcagacc catggaagaa
     1261 atcatggaag atctggaaga tgccagaagg acgtataatt ttgtcgagaa gatatttctt
     1321 tgtgacggtg atgctctatg tctttctttt gataaactga tgaccatttt gaggaaaata
     1381 aaagacattt tccccgaatg caaaagagtc actgtttacg gttcgcctca ggatgtatta
     1441 agaaagacag aagaagaact gaaggggctt tatgaggcgg gagtagaaat aatttatata
     1501 ggagccgaat ccggcagcga caaagtcttg aaaaatgtca aaaaaggtgc tgacagaaaa
     1561 gagataatag aatcggtaaa aaagatagaa gaagcgaaaa taaaagcatc agtcactttc
     1621 atatcgggac ttgggggaag agaactctgg cgggagcatg caaaagaaag cggcgccatg
     1681 ataagtgaaa tgcagccttc atatgtggga cttctgaccc ttatgacgga accggaagct
     1741 cccctgacag acgatatcgc caaaggcgga tttctgcttc ttactgcaga ggaagtggtt
     1801 gccgaaactc tgctgatgct gaagaatatc aatgtgaaga aagaatgcat attcagaagc
     1861 aatcacgcat caaactatgt ttctctgaga gggaccctgc cccgggacaa ggatatgatg
     1921 ataaagcagc tggaaaatgc cgtaaaaaat aaaggcatgc tgaaggatga aagattcagg
     1981 atgctctaaa ataaggaagg tataaatgag taacaaagca aaagaaaaac agaacggtgg
     2041 aataaaagca cccggtgaac agaaactgac catggcccag tctcttggta taacaaaaac
     2101 agtcaaaaga acagaggaag aaacagacat aagaaaaagg ccggacagga tcagcaagta
     2161 tttcaggaaa tatttgaaca aatttgtttt tgttgaattt tcagatgaat atcttaacgg
     2221 ttccaaaata aaggatataa tgaaaggtgt gccggtaccc ttaagacgca gggatgtaaa
     2281 ggaattcgcc ggtggaaaag gattgaaagc cacccatata gccgaaaata tggcatggat
     2341 catggggtgc gatccaaaat tcaaatataa tcaggcatat atcgactaca tgttcaagct
     2401 ctttaactac aagatatatg aagggatgat gaaagagggc agagatgcgg cggaaatcga
     2461 agattatgat aatgcatgca tccacttcag ggcatctctt tgcatgaaac cggattatct
     2521 ccacggcatg tacagttatg ccagagtatg cagggcaatg tatatgaaca gcgggaacac
     2581 agaatatgtt ggaaggttca aggcggaagc gctggactac tttgagcttg tcacagagac
     2641 ccatcccaga tttgctcagg gatactatta tctgggatat gcgtatctta atataggtct
     2701 ttacagcaaa gcctacgccg catggaaatg gttccttaaa ttcaccagga gcggcaaaga
     2761 cagaaaagaa ataaacaaga gaatagccca aatagagaac cctatgagga tagaagaagg
     2821 atataatgag atcctcgccg gccgttacga tcagggcgtc gatatactgg agaattatct
     2881 ggacagccag tataaggact ggtggcccct tcactattat cttgggatag gatatgcaag
     2941 gctgggcaaa aaaggtgatg cttttgccag gttcaagcac accctttctc tgaacggatc
     3001 acatcttgaa accatggaag aactggtggc aatatatgag gccgaaggag ataaagaaaa
     3061 cacgaacaaa tacagaaaaa aaatcgaact gataaaagcc gatatagcaa aggaacagga
     3121 agccataaag aaggcagcgg aaaaggaaag cggaaccgaa atagtatatt tggaacagaa
     3181 aacaaaaaac aaaaaagaaa aatctaagac aagctccaaa gaaacaaaaa aagaagaacc
     3241 ggaaaaagcc aaagagaa
//
LOCUS       DAONHK010000121         3224 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_24294_length_3224_cov_3.436415, whole genome shotgun sequence.
ACCESSION   DAONHK010000121 DAONHK010000000
VERSION     DAONHK010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3224
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24294_length_3224_cov_3.436415"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(78..1133)
                     /gene="queA"
                     /locus_tag="PLM92_04915"
     CDS             complement(78..1133)
                     /gene="queA"
                     /locus_tag="PLM92_04915"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010965581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HPR24511.1"
                     /translation="MRVSDFDYPLPEELIAQHPKDKRDGSRLIIVDRETGRTEHRHFY
                     DILDQLSPSDCLVLNDSKVIPARLFGIKEGTGAKVEFLLLKRMHGDVFEVMVKPGRKL
                     KTGDKVTFSRPEDEAGLFQAEITGYGRDGTREALFTYEGIFIEKLEKLGKMPLPPYIK
                     RDAENEDKERYQTVYCKREGSVAAPTAGLHFTDDLLKKVSDKGVKLAYLTLHVGIGTF
                     RPVKEKNVQDHHMHFEDYQIDEKAAEIINGTIEEGGRIVCVGTTSVRTLESAAEKTGG
                     SWKVKAGAGSTDIFIYPGYSFKIVDSLITNFHLPRSTLLMLVSALYTREDMLRIYREA
                     VEKEYRFFSYGDAMFIK"
     gene            complement(1145..2209)
                     /gene="ruvB"
                     /locus_tag="PLM92_04920"
     CDS             complement(1145..2209)
                     /gene="ruvB"
                     /locus_tag="PLM92_04920"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005547426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HPR24512.1"
                     /translation="MDKEDRITAAKAGGTEEKNERGLRPQNLEEYIGQKTVTDNLKVF
                     IEAARKRGEPLDHVLFYGPPGLGKTTLAGIIANELEVDIRITSGPAIERAGDLAAILT
                     NLNENDVLFIDEIQRLNRSVEEVLYSAMEDFALDIIIGKGPSARSIRLDLAHFTLIGA
                     TTRAGSLSAPLRDRFGVICKFETYSKKELKQIASRSSHVLGLEADESSLEEISGRSRG
                     TPRVVNRLLKRVRDFSEVKGTGHVEIDITKKALKALGVDDRGLENLDREILKLIIERF
                     HGGPVGIDTIAASIGEERITIEDVYEPYLIQAGFLYRTQKGRMVSEEAYRHLGLDVPE
                     GIKPPEDIPGEEEKQEKLDL"
     gene            complement(2240..2836)
                     /gene="ruvA"
                     /locus_tag="PLM92_04925"
     CDS             complement(2240..2836)
                     /gene="ruvA"
                     /locus_tag="PLM92_04925"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HPR24513.1"
                     /translation="MMFHYIRGILAAKFQGTVVIEAQGIGYEIAVPLNSRVLAAGEGE
                     ELTVFTYMAVKEDDIALYGFEDEWSLDLFKKLISVSGIGAKAGLAVLSAMSAEEIKKA
                     IIFEDADMLTRAQGIGKKSAQRIVLELKDKLGTPGENGYSKGAAAGGIFGDNEKEEAL
                     NAMIALGYSRSEAAKAMAEIKEKDLTAEEYIRVALKKL"
     gene            complement(2833..>3224)
                     /gene="ruvC"
                     /locus_tag="PLM92_04930"
     CDS             complement(2833..>3224)
                     /gene="ruvC"
                     /locus_tag="PLM92_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014967206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HPR24514.1"
                     /translation="MPLRLKYLYESLTEIIKEHKPEEAAIEELFFNTNSKTAIMVGQA
                     RGVAILACTNSGLDISEYTPLQIKQALTGQGRADKKQVQFMVKNILALSEVPSPDDVA
                     DALAAAVCHGHSWGNKKIMNDNRRGEI"
BASE COUNT          700 a          797 c          635 g         1092 t
ORIGIN      
        1 ctcttctggc ttttttgtcc ttttttatta ggttgaatgt tactgcgtct ttcattttct
       61 tccttatctg ataaatatca ttttatgaac atggcatctc catagctgaa aaatctgtat
      121 tctttttcga ctgcttctct gtatattcta agcatatcct ccctggtgta aagagctgaa
      181 accagcatca gcagcgtgga tctgggcagg tgaaaatttg ttatcaaaga gtcaacgatc
      241 ttgaatgaat aaccgggata tatgaatatg tccgtgcttc ctgcccctgc tttcaccttc
      301 cagcttccgc cggttttttc ggcggcactt tcaagggtcc ttacagatgt agtccccaca
      361 catacgatgc gtccgccttc ttctattgta ccgtttatta tctcagccgc cttttcatct
      421 atttgataat cttcaaaatg catatggtga tcctgtacgt tcttttcctt gacgggtctg
      481 aacgtgccta ttcccacatg caaagtcaga tatgccagtt tgactccctt gtcagagact
      541 tttttcagaa ggtcatcggt aaaatgaagt cctgccgtag gtgctgccac agaaccttct
      601 cttttgcagt atacggtctg atacctttct ttgtcttcat tttcggcatc tctttttata
      661 tagggcggaa ggggcatttt gcccagcttt tcgagttttt caatgaatat gccttcgtag
      721 gtaaaaaggg cttctcttgt gccgtctctt ccatagcctg ttatctccgc ctgaaaaagt
      781 cccgcctcat cctccggcct tgaaaaagtg actttatctc cggtctttaa tttccgcccc
      841 ggtttcacca tcacttcaaa gacatctcca tgcatccttt tgagaagaag aaattccact
      901 tttgcgccgg ttccttcttt tattccaaaa agtcttgcag gaatgacttt tgagtcgttc
      961 agtacaagac aatccgaagg acttagctga tccaatatat cataaaaatg cctgtgttcc
     1021 gttcttcctg tttctctgtc tacaattatt agccgggatc cgtctctttt gtctttggga
     1081 tgctgggcga tcagttcttc gggaagcggg taatcaaaat cgcttactct cataccggta
     1141 tctttcataa gtccagcttc tcctgtttct cctcttcccc gggaatgtct tccggaggtt
     1201 ttataccttc gggaacatca agccccaggt gtctgtaagc ctcctcagaa accatccttc
     1261 ccttctgggt acggtacagg aatccggcct gtatcagata gggttcataa acatcttcaa
     1321 tggttattct ttcttctcct atggaggcag ctatggtatc tattcccacg ggtcctccgt
     1381 gaaatctttc gattatgagt ttgagtatct ctctgtccag attttcgaga cctctgtcat
     1441 ctactccaag ggccttaagg gctttttttg ttatgtctat ctctacgtgt cctgttcctt
     1501 tcacctcgga aaaatctctc actcttttga gaagtctgtt gaccactctg ggtgtgcctc
     1561 tggatctgcc ggagatctct tcaagagagc tctcatctgc ttcaagacct aatacgtgcg
     1621 atgaacggct cgctatctgt ttaagctctt tttttgaata tgtttcaaat ttgcatatga
     1681 ctccgaatct gtctcttaag ggggccgaaa gacttccggc tcttgtggtg gcccctatca
     1741 gggtgaagtg ggccagatca agtcttatgg atctggcgga gggacctttg cctattatta
     1801 tatccagggc aaaatcctcc atggctgaat aaagcacctc ttccacagac cggttcagtc
     1861 tttgtatttc atcaataaaa aggacatcat tttcgttgag attggtaagg atcgctgcaa
     1921 ggtcaccggc tctttcgatg gccggccccg atgttatcct tatatcgacc tcaagctcat
     1981 ttgctattat tccggcaagg gtcgttttgc ccagacccgg aggaccgtaa aaaagcacat
     2041 gatccagagg ctctcctctt tttcgtgccg cttctatgaa aaccttaaga ttatcggtca
     2101 ctgttttttg tccgatatat tcctcaaggt tctggggcct taatcccctt tcgttttttt
     2161 cttctgttcc tccggctttg gccgctgtta tcctgtcttc tttatccata atataccttt
     2221 atcaagatca ttaagagtcc tataattttt tgagagctac tcttatatat tcttctgcag
     2281 taagatcttt ttcttttatt tcagccatgg ctttggcggc ttcgcttctg gaataaccca
     2341 gggctatcat tgcattcaat gcttcttctt tttcgttgtc gccgaatatg ccacccgctg
     2401 cagctccctt agaatatccg ttttcaccgg gagttcccag tttgtccttc agttccagta
     2461 ctattctctg ggcggatttt ttgcctatgc cctgggctct ggtcagcata tcggcatctt
     2521 cgaatatgat ggcttttttt atttcttctg cagacatggc tgaaaggaca gcaagacctg
     2581 cttttgctcc tatgccgcta acggaaatga gttttttgaa aagatccagc gaccactcat
     2641 cttcgaaccc gtaaagggca atatcatctt ccttgacggc catgtatgtg aaaaccgtca
     2701 actcttctcc ttctccggca gccaaaactc tgctgttcaa gggcacggct atttcatatc
     2761 ctatgccctg agcttctata acaactgttc cctgaaattt tgcggcaagt atacctctta
     2821 tgtaatgaaa catcatattt ctccccggcg attatcattc attatttttt tgtttcccca
     2881 tgagtgtccg tgacatactg ctgcagccag agcatcagcc acatcatcgg gactgggcac
     2941 ttccgaaaga gccagtatgt tctttaccat gaactggacc tgttttttgt cagccctgcc
     3001 ctgtccggta agagcctgtt ttatttgaag gggagtgtat tcacttatat caagacctga
     3061 attagtacat gcaagtatag ccacacccct tgcctggccc accatgatcg ccgtcttgga
     3121 atttgtgtta aaaaaaagtt cttcgatggc ggcttcttca ggcttatgtt ctttgattat
     3181 ttctgtaagt gattcataaa gatatttaag acgcaggggc attt
//
LOCUS       DAONHK010000122         3145 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_25178_length_3145_cov_5.398058, whole genome shotgun sequence.
ACCESSION   DAONHK010000122 DAONHK010000000
VERSION     DAONHK010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3145
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25178_length_3145_cov_5.398058"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..712)
                     /locus_tag="PLM92_04935"
     CDS             complement(<1..712)
                     /locus_tag="PLM92_04935"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF013640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HPR24515.1"
                     /translation="MTDHEKNPERREISRICMPITGSSKNEIIRQTEKAMTAKCGMIE
                     WRADYLWAGDPVSVHKDPLVILDILDTICRISKNAETGKSSGKEEIKNKTVIFTLRTK
                     EEGGKANLKRSAYMEIIRDVIQKSRVDMVDIELLGENGTEDKARLEFIVDIAHDEGKK
                     VILSNHDFERTPDMEVITGRMDSMKELGADIVKVAYMPCDDADVDVLMDAAESMQKKN
                     PKMPLVVISMGENGKRTRE"
     gene            complement(715..1158)
                     /locus_tag="PLM92_04940"
     CDS             complement(715..1158)
                     /locus_tag="PLM92_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008752533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HPR24516.1"
                     /translation="MDKKYDVLKLENQLCFPLYACSKEMIRLYKRFLDPLDLTYTQYI
                     AMMVMWEHKRMTVKEMGEILLLDSGTLTPVLKKLESKGYLSRKRSKEDERTVMISITE
                     KGQDLKAEAAMIPERMGECIKLSKEEASKLQKILYKTLKKMEREK"
     gene            complement(1208..1684)
                     /locus_tag="PLM92_04945"
     CDS             complement(1208..1684)
                     /locus_tag="PLM92_04945"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HPR24517.1"
                     /translation="MSIYDLNVKDMEGKEVSLSDYKDKVLLIVNTATGCGFTPQYAGL
                     QELYTKYREKGLEILDFPCNQFGNQAPGTSEEIYTFCTGRFGMSFRQFEKIDVNGPDE
                     SPLYTFLKSQKIKGNGKIKWNFTKFLVDREGNVVKRFAPVVKPEKIEADIVKLLEE"
     gene            complement(1938..3131)
                     /gene="tuf"
                     /locus_tag="PLM92_04950"
     CDS             complement(1938..3131)
                     /gene="tuf"
                     /locus_tag="PLM92_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HPR24518.1"
                     /translation="MAKEKFERNKPHINIGTIGHVDHGKTTLTAAITKTLQDRYKLGK
                     LVAFDEIDKAPEEKERGITIATAHVEYETPNRHYAHVDCPGHADYVKNMITGAAQMDG
                     AILVVAATDGPMPQTREHILLSRQVGVPYIIVFLNKCDMVDDDELLDLVEMEVRELLD
                     QYEFPGDDTPIIRGSALKALENSEGEWGDRIIELMEAVDSYIPEPKRDNDKPFIMPVE
                     DVFSITGRGTVATGRVGRGRLKVGDEVEIVGLTDEKRKVVVTGVEMFRKLLDEAETGD
                     NIGALLRGVQRDDIERGQVLAKPGSIHPHTKFKGQVYVLKKEEGGRHTPFFNGYRPQF
                     YFRTTDVTGDLKLPEGTEMCMPGDNIVMEINLITPIAIEEGLRFAIREGGRTVGSGVV
                     TEIIE"
BASE COUNT          615 a          787 c          553 g         1190 t
ORIGIN      
        1 cttcccgggt cctcttgccg ttttctccca tggatatgac caccaggggc atttttggat
       61 tttttttctg catcgattcg gccgcatcca taagaacatc cacatctgca tcatcacaag
      121 gcatatatgc caccttgact atgtctgcgc ccagttcttt cattgaatcc attctgcctg
      181 ttataacttc catatcggga gttctctcga aatcatgatt tgacagtatt acttttttgc
      241 cttcgtcatg agctatatct actatgaact ccagtctggc cttgtcttct gtaccgttct
      301 cacccaaaag ctctatgtcg accatgtcca cacggctttt ttgtatcaca tcccttatta
      361 tttccatata tgcgcttctt ttaagattgg ctttgcctcc ctcttccttt gtacgcaaag
      421 taaatatgac cgttttgttt tttatttctt cctttccgga agattttccg gtttccgcgt
      481 tttttgaaat tctacatatg gtatccagta tatcaagaat cacgagggga tccttatgta
      541 cggaaaccgg atctcctgcc cataaataat cagctctcca ttctatcatg ccgcattttg
      601 ccgtcatggc tttttctgtc tgccggatta tttcgttttt gctgctaccg gtgataggca
      661 tacatattct tgatatttcc cttctttccg gatttttttc gtgatctgtc atttttattt
      721 ctcccgctcc atttttttca aggtcttata aaggatcttt tgtagctttg atgcttcttc
      781 tttggaaagt tttatgcact cacccattct ctcaggtatc atagcagctt ctgcctttag
      841 atcttgtccc ttttcagtga tgcttatcat tactgtccgt tcatcttctt ttgagcgctt
      901 acgtgacaaa taccctttgg actcaagttt tttcagcaca ggtgtaagtg tccctgaatc
      961 aagtagaagt atctctccca tttcctttac ggtcatcctt ttgtgctccc acatgaccat
     1021 cattgctatg tactgagtat atgtaagatc aaggggatca agaaaacgct tataaagtct
     1081 tatcatttcc tttgagcacg cataaagggg aaagcacagc tggttttcca gtttgagcac
     1141 atcatatttt ttgtccattt tctgtctcct gtttgtctgt taatagatgc cgttttacat
     1201 ccggtcgcta ttcttccagt agttttacaa tgtccgcttc tatcttttcg ggtttcacta
     1261 ccggtgcaaa tctttttaca acattacctt ccctgtccac cagaaacttt gtgaagttcc
     1321 actttatttt tccgtttccc tttattttct gagatttgag gaaggtgtaa aggggacttt
     1381 cgtcgggtcc gttgacatcg attttttcaa actgtctgaa actcatgcca aacctgcctg
     1441 tacagaaagt atatatctcc tccgatgtcc ccggagcctg attgccaaac tgattgcatg
     1501 ggaaatcaag tatctcaaga cccttttccc tgtatttcgt ataaagttcc tgaagccctg
     1561 catactgggg agtgaatccg cagcctgtgg ccgtattgac aataagaaga accttgtctt
     1621 tgtaatcaga aagtgatact tctttccctt ccatatcttt tacattaaga tcataaatac
     1681 tcatttcttc tcctcctgct ctttttctgt tatctggttt ttgtttcttt atttttttct
     1741 gtttttattt aatgcattat tttgttttat gtttgaatta tattgtacgc aatttaattt
     1801 tgtttgtcaa tactttttct gtactttttc ggaaaagcaa agaccggcca gtcggccggt
     1861 cttttgtatg tcttttagtt cttttgtatt ttgtgttttc atgcttcctt atgtttgaag
     1921 catttgtgat cttacagtta ttcgatgatc tccgttacta cgcctgaacc tactgtcctt
     1981 ccgccttctc ttatggcgaa tctcagtcct tcttctatgg cgattggcgt tataagattg
     2041 atctccataa cgatgttgtc tccgggcatg cacatttctg tgccttcagg aagtttaagg
     2101 tctcccgtta cgtccgtcgt cctgaaatag aactgaggtc tgtatccgtt gaagaacggt
     2161 gtatgtcttc cgccttcttc tttcttaagt acatatacct gacctttgaa ctttgtatgg
     2221 ggatggatcg atcccggctt tgccagtacc tgtcctcttt ctatatcgtc tctctgtact
     2281 cctctgagca gtgctccgat gttgtctcct gtctctgctt catccagcag tttcctgaac
     2341 atctccactc ctgttacgac tacttttctc ttttcgtctg taagacctac gatctccact
     2401 tcgtcgccta ccttcagtct tcctcttcct actcttcctg tggctaccgt tcctcttcct
     2461 gttatggaga atacgtcttc caccggcatg atgaaaggct tgtcattatc tctcttgggc
     2521 tcgggtatat aactgtctac tgcttccatc agctcgatta tcctgtctcc ccattcgcct
     2581 tccgagtttt ccagtgcctt caacgcagat ccccttatta tcggtgtgtc gtctcccggg
     2641 aattcatact ggtcaagaag ctctcttact tccatttcta ccagatccag cagttcatcg
     2701 tcatctacca tatcgcattt gttcaggaac acgatgatat acggtactcc tacctgtctt
     2761 gacaatagaa tgtgctctct tgtctggggc atgggtccgt ctgttgctgc caccaccagg
     2821 atagctccgt ccatctgcgc tgctcctgtt atcatgttct ttacatagtc agcatgtccc
     2881 ggacagtcta catgagcata gtgcctgttg ggtgtctcat attcaacgtg ggccgtcgct
     2941 atggttattc ctctttcctt ttcttccggt gctttgtcta tctcatcaaa tgccaccagt
     3001 ttccccagct tatatctgtc ctgcagcgtt tttgttattg ctgctgtcag cgttgttttg
     3061 ccgtggtcta catgtcctat cgttcctatg tttatgtgcg gcttattcct ttcaaatttc
     3121 tcctttgcca ttttttcttc ctcct
//
LOCUS       DAONHK010000123         3064 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_26152_length_3064_cov_4.081755, whole genome shotgun sequence.
ACCESSION   DAONHK010000123 DAONHK010000000
VERSION     DAONHK010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3064
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26152_length_3064_cov_4.081755"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            153..1172
                     /locus_tag="PLM92_04955"
     CDS             153..1172
                     /locus_tag="PLM92_04955"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HPR24519.1"
                     /translation="MEIKEKTTDIPIFSSAVYDFYFGGLNTAVFDIETTGLSPENSSV
                     IMAGFFIPSQEGGIVRQYLAENLSEERDLISMILKEIKDLDVLITYNGKAFDIPFLKT
                     RASHMGLHDLYLPYTLDIYKAVKDHSPIKSFLPNLKQKTLETFMGLWENRTDLISGKE
                     SAEMYFEYINNGDKKIKEKILLHNSDDVIQLYKLLSVTDKTDFHKAMYHLGYPIKKGF
                     WSGIVSNISFSGQRLMVKGDQLDHPANYTCFAGEDNDYSSSFSSAKRKFTTEILLLNE
                     KNYLFADLRKPSIRNENSENQFKSYPGYENGYIIIRKGSDINYGETNHLVKFILEGMV
                     EKCII"
     gene            1169..2440
                     /locus_tag="PLM92_04960"
     CDS             1169..2440
                     /locus_tag="PLM92_04960"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013976237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HPR24520.1"
                     /translation="MIIAKEAKNDSFKLASTEGSVRNDALREIASSLQKNSDQIFAAN
                     TVDLEMGEKEGLPTPLLNRLKFDEHKLSDCINGIKDLVSMPDPLFDTLMKKKLDDDLI
                     LEKITCPIGVIGVIFESRPDALVQISSLCVKSGNCAILKGGSEAKNTNKALFDVIHAA
                     GTNAGLPSGFAVLIEDRAGIDELLKCHDSIDLIIPRGSNSFVQHIMSNSRIPVMGHAD
                     GVCHIYVDRDADIQKAIPVIIDSKTQYTAACNAVETLLIHKDMAEMIIPALNKTAKGK
                     IIFKGDEKAVSCSGDQWENASEEDWGKEYLDNVLSVKIVNDISDAVSHINNYGSHHTD
                     AIITENDDSADFFMNNVDSAGVYKNCSTRFADGFRYGFGAEVGISTGKLHARGPVGID
                     GLITYKYRLIGDGNIVADYATGKRDFKFIPI"
     gene            complement(2496..>3064)
                     /locus_tag="PLM92_04965"
     CDS             complement(2496..>3064)
                     /locus_tag="PLM92_04965"
                     /inference="COORDINATES: protein motif:HMM:NF015949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF364 domain-containing protein"
                     /protein_id="HPR24521.1"
                     /translation="WILTGKEDLQKAIGGAVLCAALEDTGVQDEEKTENPYGINIKTD
                     DKVGMIGFIPPVADMFMKHAGSLIVFDQGISLRGGGERGCDVKVMPMEKQQQMLPDRD
                     IVVLSGTTTVNGTIDGLLEMCKKAREIIMIGPSTPMCPEGFKGSGITRLAGSFWDRNR
                     KEEIFKTISQAGGIAHVKKHMIKKSAKV"
BASE COUNT          933 a          634 c          617 g          880 t
ORIGIN      
        1 atttttatag gcggatttgt tacattatta gtgtggttat cccctgataa cctcattaat
       61 ctctaatccc aatacccctt ttgaacaaaa cagactgctt tgcagtctgt tttgtttttt
      121 gaaatatcat tgtattgtgt tattatagtt ccatggaaat caaagaaaaa acaacagata
      181 ttcccatttt ctcatctgcc gtatatgatt tttattttgg gggccttaat acggctgttt
      241 ttgacatcga aaccacaggc ctgtctcccg aaaacagttc tgttataatg gcgggttttt
      301 ttataccctc acaggaaggt ggcatcgtgc gtcagtacct tgccgaaaac ctttcggaag
      361 aaagagacct tatttcgatg atattaaaag aaataaaaga cctggatgtc cttatcacat
      421 ataacggcaa agcatttgac ataccttttt tgaaaacacg ggcatcacac atgggcctgc
      481 atgatcttta tctgccatat acactggata tatataaagc cgtgaaggat cattccccaa
      541 taaagagttt tcttcccaat ctcaagcaaa agacccttga aacatttatg ggtctttggg
      601 aaaaccgtac agatcttata tccggcaaag aaagtgccga aatgtatttc gagtatataa
      661 ataacggcga taaaaaaatc aaagaaaaaa tactccttca caacagtgat gatgtgatac
      721 aattatacaa attacttagt gtgacagaca aaacggattt ccataaagca atgtatcacc
      781 ttggctatcc catcaaaaaa ggcttctggt ccggaatagt atcaaatatc agtttttcgg
      841 gacaaaggct catggtaaag ggggatcagc ttgatcatcc ggcaaactac acctgcttcg
      901 ccggagaaga caatgattat tcttcgtctt tttcatcagc caaaagaaaa tttaccacag
      961 agatactatt gctcaatgaa aaaaactatt tatttgctga tctgcgaaaa ccgtctatcc
     1021 ggaacgaaaa cagcgaaaat cagtttaaaa gttatcccgg atatgaaaac ggatatatca
     1081 taatcagaaa aggcagtgat ataaattacg gtgaaaccaa tcatctggta aaattcattt
     1141 tggaaggaat ggtggaaaaa tgcattatat gatcattgca aaagaagcaa aaaatgacag
     1201 tttcaaactg gcttcgactg aaggatctgt cagaaatgat gccctcagag aaattgcctc
     1261 ttcacttcag aaaaacagcg atcaaatatt tgccgcaaac acagtcgatc ttgaaatggg
     1321 ggagaaagaa ggtctgccta ctccactgct caacagactc aagttcgatg aacataaact
     1381 gtcagactgc ataaatggaa taaaagatct tgtttcaatg ccggatcctc tttttgacac
     1441 actaatgaaa aagaagctgg atgatgacct tatactggaa aagatcacct gccctatagg
     1501 ggtcatcggt gtgatatttg aatcccgtcc cgatgccctt gtccagatct cgtcgctttg
     1561 tgtaaaaagc ggcaactgtg ccatcttaaa gggtgggagc gaagcaaaga acaccaacaa
     1621 agctcttttc gatgtaatac acgctgccgg gaccaatgcg gggcttcctt caggatttgc
     1681 agtgcttatc gaggatcggg caggaataga tgagcttctc aaatgtcatg attccattga
     1741 ccttattata ccgagaggct ccaacagttt cgttcagcat ataatgtcaa attccaggat
     1801 ccctgtcatg gggcatgctg atggtgtttg tcatatctat gtggatcggg atgcggatat
     1861 ccaaaaggct ataccggtga tcatagactc aaagacccag tatactgcag catgcaatgc
     1921 tgttgaaaca cttttgatac ataaagatat ggcggaaatg atcatccctg cgctaaataa
     1981 aacggcaaaa ggaaaaatca ttttcaaagg cgatgagaag gcagtctcct gttcggggga
     2041 tcaatgggaa aatgcatctg aagaagactg ggggaaggaa tatctggata atgtactttc
     2101 ggtaaaaata gtgaatgata tcagtgacgc tgtttcccat ataaataatt acggttctca
     2161 tcacaccgat gcaataataa cagaaaacga cgattccgca gattttttca tgaacaatgt
     2221 ggattctgca ggagtgtata aaaactgttc caccagattt gccgacggat tcagatacgg
     2281 ttttggtgca gaggtgggta taagcaccgg aaagctccat gcacgggggc ccgttggaat
     2341 agatggtctt atcacatata aataccgcct catcggagac ggtaacatag ttgccgatta
     2401 tgcaacagga aaaagagatt tcaaattcat accaatataa atcgctcttt tcttaaatga
     2461 taatttgttt tataatcact tttttattga gagtttcaaa cctttgcgga tttttttatc
     2521 atgtgttttt tcacgtgggc tataccgccg gcttgagata ttgttttgaa tatctcttct
     2581 ttgcggtttc tgtcccagaa tgatcctgca agtctggtta tcccgctgcc tttaaagcct
     2641 tcgggacaca tgggtgttga aggccctatc attataattt cacgggcttt tttgcacatt
     2701 tcaagcagcc cgtctattgt tccgtttaca gtggtcgtcc cggaaagcac gactatatcc
     2761 ctatccggaa gcatttgctg ctgtttttcc atgggcatta cctttacatc acaacctctt
     2821 tctccgcctc cccgtaaaga gatcccctga tcaaaaacta tcagagaacc ggcatgcttc
     2881 atgaacatat cagccacagg aggaataaac ccgatcattc ccactttatc atcagttttg
     2941 atatttatgc cgtaagggtt ttctgttttt tcttcgtcct gcactcccgt atcttccagt
     3001 gccgcacaga ggacagcgcc acctatggct ttttgaagat cctctttgcc ggtcagtatc
     3061 catt
//
LOCUS       DAONHK010000124         3031 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_26560_length_3031_cov_3.244624, whole genome shotgun sequence.
ACCESSION   DAONHK010000124 DAONHK010000000
VERSION     DAONHK010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3031
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26560_length_3031_cov_3.244624"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2206)
                     /locus_tag="PLM92_04970"
     CDS             complement(<1..2206)
                     /locus_tag="PLM92_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014731653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HPR24522.1"
                     /translation="MKKILILILTVSVMISVMMPLLLSRSYAADKEIDWTREEAQFIK
                     NHPVIKLGVDPEFVPFEFIDEDGKYKGIAADYLDLISEKTGLKFEIQKGLTWPEAYDM
                     AVNGEIDALPLIGKTSEREEHFIFSEPYYYFKRVIVTRETEKGISNLKDLEGKIVAVQ
                     RNSSHHSYLLEHSEINISLYDSVEAGLTAVATGKERAFIGNLATTNYMIHTHGLTGLR
                     YIAFEAQKAQALHFAVRKDWPELVSIFDKAMDAVTEKEKTAINNKWVDLQTDIGYGPI
                     IRIIIIVGSVIAIVFIVSFFWIVRLQKEIRRRKKVQKDLEKAKLEADEANEFKSNFLA
                     RMSHEIRTPLNAITGISYLLKKTDISFTQSMYVDRIMRAADNMLGIVNDILDFAKIEA
                     GKIELEKVPFSMDATIREVVNIVSYKIEEQGIRFRVFKDPAIADWFIGDSNRIQQILQ
                     NVLGNASKFTKAGEISLDVSLVSKKGDTHLLRFSVRDTGIGMTEEQKQKLFSPFIQAD
                     SSINRRFGGSGLGLSIVKNLVEMMKGEVRVNSVPGEGSEFIIEISLDPDKQKQKMYQK
                     MLSMSSFKEAKVLILQKKDSDMEPVCEYLSYFGMACETVFSEKDAIQRLEASLKKSDK
                     PFDLIMTGCGKSSGSGFEFIKNLRNNTSLTEIPIVIMLIPMNRYDLFDKLHENGIKRG
                     IAQPVTPYNILNEVIDALGIKKDKADGKAYETEQWLPLKKGKYHAVLADDNKT"
     gene            complement(2322..2630)
                     /locus_tag="PLM92_04975"
     CDS             complement(2322..2630)
                     /locus_tag="PLM92_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013656981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4491 family protein"
                     /protein_id="HPR24523.1"
                     /translation="MDFQGLIIGVITFVIIGIFHPIVIKSEYYIGKKIWPAFLALGIL
                     CIVLSLFANNVVVSAVFGITGFTFFWSIKEIIEQEERVKKGWSPANPKKTVVEYFYGY
                     "
     gene            complement(2744..>3031)
                     /locus_tag="PLM92_04980"
     CDS             complement(2744..>3031)
                     /locus_tag="PLM92_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012464608.1"
                     /note="has catalase and peroxidase activities; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase-peroxidase"
                     /protein_id="HPR24524.1"
                     /translation="EKKGVLSNDFFINLLDMDTEWKPVSEERDLFEGRDRKTGDLKWT
                     ATRVDLIFGSNSELRAISEVYGADDAKEKFVSDFICAWNKVMNADRFDLAI"
BASE COUNT          786 a          671 c          522 g         1052 t
ORIGIN      
        1 ttgttttatt gtcatctgca agaactgcat gatattttcc tttcttcaac ggtagccatt
       61 gttctgtttc gtatgctttt ccgtctgcct tatccttttt tatccccaga gcatcgatga
      121 cctcattcaa aatattgtat ggggtcactg gctgggctat cccccttttg ataccgtttt
      181 catgaagctt atcaaaaaga tcatatctgt tcatgggtat cagcattatt acaatgggaa
      241 tttcggttaa gcttgtgtta tttcggagat tctttataaa ttcaaatccg cttccggaac
      301 ttttgccgca accggtcata ataaggtcaa aaggtttatc tgattttttt aaagaagctt
      361 ctaatctctg tatcgcatcc ttttcagaaa aaactgtttc gcatgccatg ccgaaatagg
      421 aaagatactc acataccggc tccatatcgg aatccttttt ttgaagtatg agtacctttg
      481 cttctttaaa ggaactcatt gaaagcattt tctgatacat tttttgtttt tgtttatccg
      541 ggtccagaga aatttctatt atgaattcac tgccctctcc cggaacgcta ttcactctta
      601 cttcaccttt catcatttca acaagatttt ttactatgga aagcccaagt cccgatccgc
      661 caaagcgtct gtttatgctg ctgtctgcct gtatgaaagg agaaaaaagt ttttgctttt
      721 gttcttctgt catgcctatt cccgtatccc tgactgagaa tctcaaaaga tgagtatctc
      781 ctttttttga tacgagactt acatccaaag atatttctcc tgcttttgta aatttggatg
      841 cattaccaag cacgttctga agtatctgct gtatcctgtt tgaatctcct ataaaccagt
      901 cggctatagc cgggtctttg aatacccgga atctgatacc ctgttcttcg atcttgtaag
      961 aaactatatt cacgacttcc cgtatggttg catccatgct gaaaggcact ttttcaagtt
     1021 ctattttgcc tgcttcgatt tttgcgaaat ccagtatatc atttactatc cccagcatat
     1081 tatcggcagc tctcattata cgatccacat acatgctctg tgtgaatgat atgtctgttt
     1141 ttttgagaag atatgaaatt cctgttatgg cattcagtgg cgttcttatt tcatgggaca
     1201 tcctggcaag aaaattcgat ttgaattcgt tggcttcatc ggcttccagt tttgcttttt
     1261 ccagatcctt ctgtaccttt tttcttctcc tgatttcttt ttggagtctg actatccaga
     1321 aaaacgaaac aataaatacg atcgcaataa cagaacctac tataataatt atcctgataa
     1381 tcggtccgta gccgatatcc gtctgcagat ctacccattt attgtttatg gcagtttttt
     1441 ccttttctgt aactgcatcc atggctttgt caaaaatact tacaagttca ggccagtctt
     1501 tgcgtacggc aaaatgaagg gcctgcgctt tttgagcctc gaaggcaata tacctgagtc
     1561 ccgtcaggcc atgtgtgtgg atcatgtagt tagtagtggc cagattgccg ataaaagctc
     1621 tttctttccc tgtggccaca gctgtaagac ctgcttctac agaatcataa aggcttatat
     1681 ttatttccga gtgttccaga aggtaactgt ggtgggaact gtttctctgg acagcgacta
     1741 tttttccttc aagatccttt aaatttgata tgcctttttc agtttccctt gtcactataa
     1801 ctcttttgaa ataataatat ggttccgaaa aaataaaatg ctcttcccgc tctgaagttt
     1861 tgccaatgag cggcagggca tctatttcgc cattaacggc catatcatat gcttcgggcc
     1921 atgtaagccc tttttgtatt tcgaatttaa gcccggtctt ctcgctgata aggtcaagat
     1981 aatctgcggc tatccctttg tacttcccat cctcatcgat aaactcaaag ggtacgaatt
     2041 cgggatcaac gccaagtttt atgaccggat ggttctttat aaactgcgcc tcttcccggg
     2101 tccagtctat ctctttgtct gcagcataag aacgagataa cagtaatggc atcattactg
     2161 agatcatgac agaaacagtt agaataagga taaggatctt tttcatctct tctccccttt
     2221 tttatgccgg ggtttcagca cattcccttc aggccttctg tcttatatgc acatttcact
     2281 atttgaactc tttcttattc tgattatatg ccttttttac atcaatatcc ataaaaatat
     2341 tcaacgacag ttttttttgg attggccggt gaccagcctt ttttgacacg ttcttcctgc
     2401 tcaataattt cttttatgct ccagaaaaaa gtaaaaccgg ttattccgaa aacagcagaa
     2461 actacaacat tgtttgcaaa taaagaaaga acaatgcaca aaatacccaa agcaagaaaa
     2521 gccggccata tttttttacc tatatagtac tcggacttta tcactatcgg atgaaatatt
     2581 cctattatga caaaggttat gactcctatt atcaatccct gaaaatccat attgttcctt
     2641 tctgcaatct gatgtcatgg atcatgcatc caaaaaactg atgtctttta agaaatccaa
     2701 aggacagtaa acctgacctt tggatttctt gtgtttagtt gtcctatatt gcaagatcaa
     2761 aacgatccgc attcatgact ttgttccatg cgcagataaa atcactgaca aatttttctt
     2821 ttgcatcgtc agcaccgtaa acctcagata tggctctcag ttctgaattc gatccgaata
     2881 tgagatccac cctggtggct gtccacttca aatcgcctgt ttttctgtct cgtccttcaa
     2941 acaggtctct ttcctcggaa acgggtttcc actctgtatc catatcaaga aggtttataa
     3001 agaagtcatt gctgaggact cctttttttt c
//
LOCUS       DAONHK010000125         3013 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_26805_length_3013_cov_3.128803, whole genome shotgun sequence.
ACCESSION   DAONHK010000125 DAONHK010000000
VERSION     DAONHK010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3013
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26805_length_3013_cov_3.128803"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1167)
                     /locus_tag="PLM92_04985"
     CDS             complement(<1..1167)
                     /locus_tag="PLM92_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002845574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine gamma-lyase family protein"
                     /protein_id="HPR24525.1"
                     /translation="MDIYTEKLLTEKFKIDKKVISTVASSENKIADIFAGLDDIMTFN
                     QYKILDAFRKNRISDVHFSWNTGYGYDDPGREALEKVWADVFSAEAALVRPIIVNGTH
                     ALSLTLSGILRPGDELIYCTGRPYDTLEETIGIRGSGKGSLKDFGIIYKQVELAEDGS
                     LDFDALKKAITPDTRMISLQRATGYAFRKAVTIPEIKAWTAFVKGIRPDIVCMVDNCY
                     GEFLDTLEPTDLGADIIAGSLIKNPGGGLALTGGYITGRKDLVDLVSYRMTAPGIGGE
                     CGLTFGQTRNMLQGLFMAPSTVNGALKGAVLCGQVFSDLGYEVCPLPDDKRSDIIQAV
                     KLGSAEAVKAFCQGIQAAAPVDSHVRPEPWEMPGYEDDVIMAAGAFVQGSSIELS"
     gene            complement(1344..1820)
                     /locus_tag="PLM92_04990"
     CDS             complement(1344..1820)
                     /locus_tag="PLM92_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPR24526.1"
                     /translation="MKTKVLILSECMKCLYPKGTETLEDFMDQLEKTERTFIEMYKLD
                     DDKTIDPYYVEEDITKIFINFDSVESVSEGEVEILKAEQFDRILSKLAKICCSDCEND
                     GESETGCDSLPGKRDKIDLETKSCWLKEECFDEDPDPDPDPDKKIVFINKGRNNNN"
     gene            complement(1820..2983)
                     /locus_tag="PLM92_04995"
     CDS             complement(1820..2983)
                     /locus_tag="PLM92_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006000693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HPR24527.1"
                     /translation="MGVQKQIKKQIKIHNKSDKPDNIVLKEHEIVSRCEALEKDLPAF
                     MRGFFVYLKGNVLPMTRLAYLYDITFFCDYLVNQTDLTAAETPKNIKLQEFERIKAVD
                     INIFIDFCRKYEKETKNAVYIYENNNKTLARKKSSVSVLFKQLYRDELISRNITDGFD
                     PIKVPKPGEKEIKALQDDEVMVMLDAVSTGAGLTKKEHEFWEKTKKRDKSILILFLTY
                     GLRLSELQQLNISSFNFNRGEFRIYRKRGKESSMPLNRSVTATVRDYIENERPGSESI
                     PEDMKDALFLSLQGKRMTERQIRELVKKYTSIGMKTSRNAGYSPHKLRATAATSLIGR
                     GNSIFDVQNLLDHEQITTTQLYAAHKMNVKRELVKGMEWEIERKEKATGKDDE"
BASE COUNT          654 a          691 c          616 g         1052 t
ORIGIN      
        1 cgaaagttca atggaggagc cctgcacgaa agctcctgcg gccataatga catcatcttc
       61 atagccgggc atttcccagg gttcgggtct tacatgggaa tccacgggag cggcagcctg
      121 tattccctgg cagaaagcct tcacggcttc tgcagagccc agttttactg cctgtattat
      181 atcacttctt ttatcatccg gaagcggaca tacttcatac cccagatccg aaaaaacctg
      241 tccgcagagt acagcgccct tcaaggctcc gtttaccgtt gaaggagcca taaacagtcc
      301 ctgcagcatg tttcttgtct ggccgaaggt gagaccgcat tcgccgccaa tgcccggggc
      361 agtcattctg tatgaaacca gatccaccag atcttttctg ccggttatat aaccgcctgt
      421 aagtgccagc cctcctcccg ggttttttat aagggatccg gctattatat ctgctcccaa
      481 gtctgtgggt tccagagtgt ccagaaattc gccgtaacag ttgtcgacca tacagactat
      541 gtcgggtctg atacccttga caaaggccgt ccaggccttt atttcgggga tagtaacggc
      601 ttttctgaaa gcatatcccg ttgcccgctg caatgatatc atcctggtat cgggggtaat
      661 agcttttttt agcgcatcaa agtctaatga accgtcttct gccagctcca cctgtttgta
      721 tattatcccg aaatccttga gactgccctt gccgctgcct cttatgccta tggtctcttc
      781 cagggtgtca tagggcctgc cggtacagta tataagctca tcaccgggcc ttaagatacc
      841 tgaaagagta agggaaagcg catgggtgcc gtttactatt atgggtctta caagggcggc
      901 ttccgctgag aaaacatcag cccatacttt ttccagggct tcccttccgg gatcgtcata
      961 tccgtaaccg gtattccagg aaaaatgcac atcgcttatc ctgttcttgc ggaaggcatc
     1021 aagtatcttg tactggttga aagtcattat atcatcaagc ccggcaaaga tatcggcgat
     1081 cttgttttct gaagatgcca ctgtgctgat gacttttttg tctattttga atttttctgt
     1141 taaaagtttt tctgtataaa tatccacttt ttattatctc tttctttgat ataagttatt
     1201 tcagttgctt cgattattgg tgttccttct tttgctttag ttattattgt tcttcttttg
     1261 attcagttat cgttgttttt tattttgatt cggttattat tgtttcttct tttgcttcgg
     1321 ttattattgt tttttctttt gcttcagtta ttgttgtttc ttcctttgtt tataaagact
     1381 atctttttgt ccgggtcggg gtccgggtcc ggatcttcat cgaaacattc ttccttaagc
     1441 cagcagctct ttgtttcaag atcgatcttg tctctttttc ccggaaggga atcacatccg
     1501 gtttcacttt ctccgtcatt ttcgcagtca ctgcagcaga tttttgctag tttggacaga
     1561 atacggtcaa actgctctgc ttttagtatt tcgacttccc cttccgatac ggattccacg
     1621 gaatcaaaat tgatgaatat cttggttata tcttcttcca cataataagg atcaatggtt
     1681 ttatcatcat ccagtttata catttcgatg aatgtccttt ctgttttttc cagctggtcc
     1741 ataaaatcct ccagagtctc tgtgcccttc gggtaaagac atttcataca ttccgaaagt
     1801 atcagtactt ttgttttcat tattcatcat cctttcctgt tgctttttct ttccgctcta
     1861 tttcccactc catgcctttg acaagttctc tttttacatt cattttgtgg gccgcataaa
     1921 gctgtgttgt ggttatctgc tcatgatcca gcagattttg tacatcgaat atggaatttc
     1981 cgcggccgat caggctggtt gcggcggtgg ctctcagttt atggggactg taacctgcat
     2041 ttcttgaagt tttcatgcct atggaggtgt atttctttac cagttccctt atctgcctct
     2101 ctgtcatccg cttgccctga agagaaagaa aaagtgcgtc tttcatatct tcggggatgc
     2161 tttcactgcc gggcctctcg ttttctatgt agtctcttac ggtggcggtg acggatctgt
     2221 tcagtggcat tgatgattcc ttgcctcttt ttctgtatat cctgaactct cctctgttga
     2281 aattgaatga ggaaatattc agctgctgga gctcggaaag tctcagcccg taggtcagga
     2341 aaagaataag tatcgacttg tctctctttt ttgtcttttc ccagaattca tgttcttttt
     2401 ttgtcaggcc tgctccggtg gaaaccgcat ccagcattac cataacttcg tcatcctgaa
     2461 gagcttttat ctccttttca ccgggttttg gtacttttat ggggtcgaaa ccgtcggtta
     2521 tgttgcggct tatgagttca tccctgtaaa gctgcttgaa aagcaccgaa acagaggatt
     2581 ttttgcgggc aagagtcttg ttattgttct catatatata cacggcgttt ttggtttctt
     2641 tttcatattt tctgcaaaag tcgataaaaa tattgatatc aacggcctta atacgttcaa
     2701 attcctgcag tttgatgttt ttgggtgttt ccgccgccgt aagatctgtc tgattcacca
     2761 ggtaatcgca gaaaaatgtt atatcgtaaa ggtaggcaag tcttgtcatg ggcagaacgt
     2821 ttcctttgag atacacaaaa aaccctcgca taaaagcagg aagatctttt tcaagagctt
     2881 cgcatctgga tactatttca tgctctttca aaacgatatt atcgggtttg tcgctcttgt
     2941 tatgtatttt tatctgtttt tttatctgtt tttgaacacc catgaaagaa tatcctccaa
     3001 tgcttcctgt tcc
//
LOCUS       DAONHK010000126         3009 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_26858_length_3009_cov_3.338524, whole genome shotgun sequence.
ACCESSION   DAONHK010000126 DAONHK010000000
VERSION     DAONHK010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3009
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26858_length_3009_cov_3.338524"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="PLM92_05000"
     CDS             complement(<1..206)
                     /locus_tag="PLM92_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPR24528.1"
                     /translation="MTKALKKEAAGFNPVRSLIAVLIVIGVAYLISLIMPNDPEKFGA
                     WSLVPALFLIVYIFITKRILESLVL"
     gene            complement(457..1941)
                     /locus_tag="PLM92_05005"
     CDS             complement(457..1941)
                     /locus_tag="PLM92_05005"
                     /inference="COORDINATES: protein motif:HMM:NF015515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HPR24529.1"
                     /translation="MTKALKKEAAGFNPVRSLIAVLIVIGAAYLISLILPNDPEKFGA
                     WAVVPAVFLIVYIFITKRILESLVLASVIGFIMIGSTGNILQVFSDSLLGVMMSEDIA
                     WLIIVCGLMGSIITLIERAGGAFAFAEWAATKAKSRKSSLLWAWVLGIIIFIDDYLNS
                     LTVGSCMSPLTDKHKVPREFLAYVVDSTAAPLCVLIPISTWAVFCGRLLETNGWATDG
                     DGLLYFIKTIPFNFYGWLAAIIVPLVILGIIPIFGPMKKAEKRVAEGGLLAPPGSDKI
                     DIHGGVQMELPDNPKMMNFFLPIIVLVASTIFFDVDMAMGVLCTMVFMFVTYLAQKLM
                     SAQEFADITIKGIQNMILPLMLMVLAFVFADVNDQIGFTRYCIESATAVMTPALMPFI
                     IFCVLAVTEFITGTNWGMYVIALPIVIPLAMELGVDMPLAVSAVLSAGVFGSHICFYS
                     DATVLTSAATGCNNFDHAFTQAPFGILAAVISGILFLIFGFVMA"
     gene            complement(2094..2237)
                     /locus_tag="PLM92_05010"
     CDS             complement(2094..2237)
                     /locus_tag="PLM92_05010"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPR24530.1"
                     /translation="MISGYAHCSRILVKPGQNVKAGQKIAKIESSGDARSPNPHIFIL
                     KSY"
     gene            2362..>3009
                     /locus_tag="PLM92_05015"
     CDS             2362..>3009
                     /locus_tag="PLM92_05015"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006572257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HPR24531.1"
                     /translation="MDRTILHCDMNGFFASVELLDHPELKNKPMAVCGNPRNRHGIIL
                     AKNEIAKKFGIVTAETLWQAKKKCPGLICVSPHMGKYKKYSRIINNIYERYTDMVEPF
                     SIDESWLDVSASMKLFGSGREIADAIRITIKEELGLTLSVGVSFNKIFAKMGSEYKKP
                     DATTVISRENYREILWPLPVEKMFFVGSASAEKLIKTGILTIGDLALAPKRHMISL"
BASE COUNT          913 a          648 c          623 g          825 t
ORIGIN      
        1 aggaccaggg attccagtat cctcttggtt ataaatatat agacgataag gaataaggcg
       61 gggaccagag accatgctcc gaatttttcc ggatcattgg gcatgatcag tgatataagg
      121 tatgcaactc cgatcactat cagaactgca atcagggatc gcaccggatt aaatccggcc
      181 gcttcttttt ttagtgcttt ggtcattttt tcctcctttc gtattaaaac aaacatcagc
      241 gtataacaac aaagttattt tataaacggt ttttacagtt aaacaatagt aaaaaagtac
      301 taaacttcaa aaaagaattg gaactttttt attacgattc ctttttttta aggctttaga
      361 gatatgaaaa agccggacga atagtccggc ttttcagcac tttctgaaaa attacatgat
      421 tttcagaatg caatattatt cattacttat tgtaacttat gccattacaa atccgaatat
      481 gaggaaaagt attccggata taacggcagc caatatacca aagggtgcct gtgtaaacgc
      541 atgatcgaag ttgttgcatc ctgttgccgc ggatgtaagt accgtagcat cagagtagaa
      601 acatatatga cttccgaaaa ctccggcaga cagtaccgct gatacggcaa gaggcatatc
      661 aacgcccagt tccatggcaa gaggtataac tataggaagt gcgataacat acattcccca
      721 gttggtgcct gttatgaatt ctgtaacagc cagaacacaa aagattatga atggcataag
      781 cgcaggtgtc attacagccg tagcggattc tatacagtat ctggtgaatc cgatctggtc
      841 atttacatcc gcaaagacga atgccagcac catcagcatc aggggcagta tcatgttctg
      901 tatgcccttt atggtgatat ccgcaaattc ctgagcagac atcagctttt gggcaagata
      961 ggtaacaaac atgaatacca tcgtacaaag aacacccatc gccatatcca catcaaagaa
     1021 gatggtcgag gcaacaagaa cgattatggg aaggaagaaa ttcatcatct ttggattatc
     1081 gggcagttcc atctggactc cgccgtggat atctatcttg tccgatcccg gaggtgccaa
     1141 aagtcctccc tccgccacac gcttttcagc ctttttcatc ggtccgaaga tgggtattat
     1201 ccccaggatt accaggggta cgattattgc tgccagccag ccgtaaaaat tgaagggtat
     1261 cgttttaata aagtataaaa gtccgtctcc atcggtggcc cagccgtttg tctcaagaag
     1321 tctgccacag aaaaccgccc atgtcgaaat gggtatcaat acgcaaaggg gtgccgcagt
     1381 ggagtccacc acatatgcaa gaaattctct gggtactttg tgcttgtctg taaggggtga
     1441 catgcaggat cccacagtca gcgagttgag ataatcgtct atgaatatta tgatacccag
     1501 tacccatgcc cacaaaagtg aggattttct ggattttgcc ttcgttgccg cccattcggc
     1561 aaaggcgaaa gcacctccgg ctctttcgat aagggtgatt atgctcccca taagtccgca
     1621 tacaatgata agccatgcta tgtcctcgct catcattact cccagcagtg aatcactgaa
     1681 cacctgcagg atgttgcctg tactgcctat cataataaat cctatgactg atgccaggac
     1741 cagggattcc agtatcctct tggttataaa tatatagacg ataaggaata cagcaggtac
     1801 cacggcccat gctccgaatt tttccggatc gttgggaagg atcagtgata taaggtatgc
     1861 agccccgatc actatcagaa ctgcaatcaa agaccgcacc ggattaaacc cggccgcttc
     1921 tttttttagt gctttggtca tatgagttct cctctcttaa aataataata agtagaataa
     1981 tatgttatta ttctacttat tttcttatat tcatgcaagt ataaattttt aaaatattcg
     2041 cattatttat tgtatttttt aatagaataa tgccggatca acaaattttc cttttaatat
     2101 gacttcaaaa tgaagatgtg gggatttggg gatctggcat cgcctgaaga ctcgattttt
     2161 gctattttct ggcctgcttt tacattttga ccgggcttga ccagaattct gctgcaatgg
     2221 gcatatcctg aaatcatgtc ttttgcccgg ttcaagtccc tctgtcttgt atgaaagaca
     2281 gccggccgga aataaacaca aaagttttct tctcatcaca ttgacctcca aaataacatt
     2341 tgtttattat ataatatgat tatggacaga acaatactgc attgcgacat gaacggcttt
     2401 tttgcatccg ttgagttgct ggatcatcct gaattaaaaa ataaacccat ggcagtctgc
     2461 ggaaatcccc ggaaccgtca cggtattata ctggcaaaaa acgaaatagc aaaaaaattc
     2521 ggaatagtca ccgccgaaac cctgtggcag gccaaaaaaa aatgccccgg tcttatatgc
     2581 gtaagccctc atatgggcaa atacaaaaaa tacagtcgca taataaacaa tatttatgaa
     2641 cgctacacag atatggtgga gccgttcagt atagatgaat cgtggcttga cgtttcggcc
     2701 agcatgaaac ttttcggttc cggcagagaa atagccgatg ccataagaat aaccataaaa
     2761 gaagagttgg gactcaccct ttctgtggga gtctctttca acaagatatt tgcaaaaatg
     2821 ggcagcgaat acaagaagcc ggatgcaacg actgtgatat cacgcgaaaa ctacagagag
     2881 atactttggc cgctgcctgt ggaaaaaatg ttttttgtag gcagtgcatc cgctgaaaaa
     2941 ctgataaaaa ccgggatact taccataggc gatctggctc ttgcaccaaa gagacatatg
     3001 atttccctc
//
LOCUS       DAONHK010000127         3001 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_26969_length_3001_cov_4.428038, whole genome shotgun sequence.
ACCESSION   DAONHK010000127 DAONHK010000000
VERSION     DAONHK010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3001
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26969_length_3001_cov_4.428038"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1839)
                     /locus_tag="PLM92_05020"
     CDS             complement(<1..1839)
                     /locus_tag="PLM92_05020"
                     /inference="COORDINATES: protein motif:HMM:NF012239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HPR24532.1"
                     /translation="MKVHELAKKLDMSSKELIEKAKSIGIEVTSANSALSEIDEKTLE
                     NAIKAKRKKDTETKIVRVASKKKDVDEKEPRVTVRAANLKTPAEKTSKVRKKTAEEEP
                     VITRKPPIGKPVVPKEVEKRAARPVGKPLPKKAGGEEEATEKPTVTKKAAIAKKAEEA
                     NKTFKKEQSEKPRVKIIKSAKEQKAEAEAKEKKEREKRAEAKRTKTGDERLSGRSERP
                     KAGIERQKDGTERPKARTDRTSAKTERPARKPSSKEATAEKPAAKKEDRTKKGKAKEK
                     ERDKFNKLEKGKRSRRPAGYEALSLEKKAKKKHVKQKISEEPEIEETPLPAGTKVINV
                     PITVGGLAEQIDVSVSKIIMTLMGLGKITNINVNIDEDTLLILAEELGMNIVIGKVDQ
                     ETEEEGIELFDDREADLMPRPPIITVMGHVDHGKTSLLDAIRKTNVTASESGGITQHI
                     GASEVEINDKRIVFLDTPGHEAFTAMRARGADVTDIAVLVVAADDSVKPQTIESISHA
                     KAAGVPIIVAINKIDKPGANPDKVKQDLTEHSILVEEWGGDVIAVPVSAKSGEGIRNL
                     LEMILLQAEVLELKANPDRLAVGTVIEARLDKAKGPVATVLVTNGTL"
     gene            complement(1843..2172)
                     /locus_tag="PLM92_05025"
     CDS             complement(1843..2172)
                     /locus_tag="PLM92_05025"
                     /inference="COORDINATES: protein motif:HMM:NF004078.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HPR24533.1"
                     /translation="MRQKIESYLGLAKKAGKLLAGYNKCLDAIEKGKIKLLIISEDVS
                     ENSMKKMIKLAETKKTKYRIYGQSEETGRITGNPGRGIFGITDKNFAEVIIKEIDQKE
                     FEKKEVF"
     gene            complement(2156..2431)
                     /locus_tag="PLM92_05030"
     CDS             complement(2156..2431)
                     /locus_tag="PLM92_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007046646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HPR24534.1"
                     /translation="MKNKKIPMRRCVGCMSSRPKNELIRIAAYEGDVSVDPTGKAKGR
                     GVYVCPDLKCLIKAEKRNALGRSLSMEISKEQMEKLFEELRTYEAKD"
     gene            complement(2421..>3001)
                     /gene="nusA"
                     /locus_tag="PLM92_05035"
     CDS             complement(2421..>3001)
                     /gene="nusA"
                     /locus_tag="PLM92_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015525794.1"
                     /note="modifies transcription through interactions with
                     RNA polymerase affecting elongation, readthrough,
                     termination, and antitermination; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HPR24535.1"
                     /translation="REAGSRTKMAVYTEDENVDPVGACVGSRGSRVQNIVDELFNEKI
                     DIITWSEDPVALISNVLSPAKVEEVMIEDEEKVATVIVPDYQLSLAIGKSGQNVRLAA
                     KLCGWKIDIKSHTQYYGDDEENDEEYEENEDTEEISEDISEEEISEEQEEMTEAGAET
                     EPDEEETEETPAGTEKETPAEKEETTETTDEE"
BASE COUNT          540 a          750 c          526 g         1185 t
ORIGIN      
        1 aagggtgccg tttgttacaa gcacggtagc aacgggtccc tttgctttat caagccgggc
       61 ttcaataact gttcccacgg ccagccgatc gggatttgct tttagttcaa gtacttctgc
      121 ctgcagaagt atcatttcaa gaaggtttct tatcccttct ccgctttttg cagaaaccgg
      181 aactgcaata acatctccgc cccattcttc cacaagtata ctatgttctg taagatcctg
      241 tttgaccttg tcgggatttg cacccggttt gtcaattttg tttattgcaa ctattatggg
      301 tactcccgca gcctttgcgt ggcttatgga ttcaatagtc tggggcttta cgctgtcgtc
      361 cgcagcaaca acaagaactg caatatcagt cacatcagcg cctcttgctc tcattgctgt
      421 aaaagcttca tggcccggcg tatcaagaaa tactattctc ttatcattga tttcaacttc
      481 ggaagcacct atatgctgag tgatgcctcc ggactcggaa gctgtcacgt ttgtttttct
      541 tatggcgtca agaagagagg tctttccgtg atccacgtgc cccattaccg ttattatagg
      601 aggtctgggc ataagatccg cctctctgtc atcaaacagc tcaataccct cttcctcggt
      661 ttcctgatcg accttgccta taacaatatt cattcccagt tcttccgcca gtataagcaa
      721 tgtgtcttca tcaatattca catttatatt tgttatcttg ccaagaccca taagtgtcat
      781 tattatcttt gaaaccgaaa catctatctg ctctgccagt ccccctactg taatgggcac
      841 atttatgact tttgtccctg ccggcaacgg tgtttcttct atctccggct cttccgatat
      901 cttttgtttc acatgctttt tcttcgcttt tttttcaagg gaaagagctt catatcctgc
      961 aggtctgcgg ctccttttgc ctttttccag cttgttgaat ttatctcttt ctttttcttt
     1021 ggctttgcct ttctttgtcc tgtcctcttt ttttgctgcg ggcttttcag cagtagcctc
     1081 ttttgaggac ggttttctgg caggtctttc ggtttttgcc gaagttctat cggttcttgc
     1141 ttttggtctt tcggttccgt ccttttgtct ttcgatcccg gcttttggcc tttcggatct
     1201 gcctgaaagt ctttcatctc cggttttagt tcttttagcc tccgctcttt tttctctttc
     1261 ctttttctct tttgcttcag cttcagcttt ctgctctttt gcagatttga ttattttcac
     1321 cctgggcttt tcgctctgtt cttttttaaa ggttttattg gcttcttcag cttttttcgc
     1381 tatggcagcc ttcttcgtaa cagtgggttt ttcagtcgct tcttcttctc ccccggcttt
     1441 ttttggcaaa ggtttgccaa caggcctggc ggcccttttt tctacttctt ttggaacaac
     1501 cggctttcct atgggtggct tccttgtgat aacgggttcc tcttctgcag tcttttttct
     1561 tacctttgaa gttttttccg caggagtttt taaattggct gctctaacag taacacgggg
     1621 ttctttctca tcaacgtctt tcttttttga tgcgaccctt actatttttg tctcggtgtc
     1681 ctttttcctt tttgctttaa tggcattttc aagtgtcttt tcgtcaattt cagataatgc
     1741 gctattggcc gaagtcactt cgatccctat tgattttgct ttttctataa gttctttact
     1801 gctcatatca agcttttttg ctaactcatg tactttcatt gtctagaaca cctccttttt
     1861 ctcaaattct ttttgatcta tttcttttat tataacctca gcgaaatttt tgtctgttat
     1921 accgaatatc cctcttccgg gattaccggt aatacggccg gtttcttcgc tttgtccata
     1981 tatcctgtat tttgtttttt tggtttccgc cagcttaatc atttttttca tgctgttctc
     2041 cgatacatct tcgcttatta ttagaagttt tattttgcct ttttcaattg catcaaggca
     2101 tttattatat ccagccagaa gctttccggc tttttttgca agtccaagat agctttcaat
     2161 cttttgcctc atatgttctc agctcctcaa aaagtttttc catctgttct tttgatattt
     2221 ccatggaaag gcttctgccc agagcgtttc ttttctcggc ttttataagg cacttcagat
     2281 cggggcagac atataccccg cggcctttgg ccttccctgt gggatcaaca cttacatctc
     2341 cttcgtaagc agcgatcctt atgagttcat tctttggtct ggatgacatg catccgacgc
     2401 atcttctcat tggtattttt ttattcttca tctgttgttt ctgttgtttc ctctttttcc
     2461 gcgggagtct ctttttctgt gcctgccgga gtttcttctg tttcttcttc atccggttct
     2521 gtttctgcac cggcttctgt catttcttcc tgttcttctg aaatctcttc ttcacttatg
     2581 tcttcagata tttcttccgt atcttcattt tcttcatatt cttcatcatt ttcttcatcg
     2641 tcaccatagt attgagtatg actctttata tctattttcc agccacagag ttttgctgca
     2701 agtcttacat tctgaccgga tttgcctatg gcaagagaaa gctgataatc gggtacgatt
     2761 acagttgcca ccttttcttc atcttcgatc atcacctctt caaccttggc cgggctcagt
     2821 acattactta tcagggcaac gggatcctcg ctccaggtta ttatatcgat tttttcatta
     2881 aaaagttcat ccactatgtt ctgaacccgg gaacctctcg aacccacaca tgcacctacc
     2941 ggatcaacgt tttcatcttc tgtgtataca gccatttttg tcctggaccc cgcttctctg
     3001 g
//
LOCUS       DAONHK010000128         2969 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_27404_length_2969_cov_3.381263, whole genome shotgun sequence.
ACCESSION   DAONHK010000128 DAONHK010000000
VERSION     DAONHK010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2969
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27404_length_2969_cov_3.381263"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            339..785
                     /locus_tag="PLM92_05040"
     CDS             339..785
                     /locus_tag="PLM92_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013725473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HPR24536.1"
                     /translation="MRYSRQNKVLELINTQEIETQEKLCQLLKDAGYDVTQATVSRDI
                     KELQLIKTLSASGNYKYAAGVNFDGPITDRFVKIFKETIKSIDSAGNIIVVKTLSGCG
                     PAAGEAIDSLALPHIVGSVAGDNTIMIVIDDPGHADEVLKEFRKFL"
     gene            813..2432
                     /gene="recN"
                     /locus_tag="PLM92_05045"
     CDS             813..2432
                     /gene="recN"
                     /locus_tag="PLM92_05045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HPR24537.1"
                     /translation="MITHLSIKNFAIISDIDIDFYKGLNIMTGETGAGKSIVIEAMNI
                     ALGGRADRTFIRTGCDKAVVQLVASHINTETVVTRELYAEGKSLCRIDGEIVSLAQLN
                     EYCQKITDVHGQYAHQSLLDPGNHITLIDSYEKESIVSTKKRVSELYDDFSEVSKRLA
                     EIHSSVREEMRKKDYMEFELKEIKKAALVPGEEEKLKEQLDTLKYMETVSNNMEKAYM
                     LAREDENSPLESLGAIQNILKEIAPHSKDASELENDFHDIYYRLEDICTRLRQAKEKI
                     IFSSSDLDELSARLDLIETMKRKYGDDVEQILDHASKLEKALSIIENLDTDTAALNLE
                     KSRIEDMLKDETRRLSSLRKAAANTLEEKINKELLDLNFQDAQISIAIKPLSGYTANG
                     TDDVEFLIKTNKGSDFKPLAKIASGGEMSRIMLAFKTVIGDYDDIPTMVFDEIDSGIS
                     GATASVVGKKLMELAGKHQIISITHLPQIAACGDHNFRIEKKSSETETFTTIKHLSEK
                     EKVNEIARLLSGSNVSDLSVKSAKELIELSQ"
     gene            complement(2529..>2969)
                     /locus_tag="PLM92_05050"
     CDS             complement(2529..>2969)
                     /locus_tag="PLM92_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018658657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HPR24538.1"
                     /translation="GLDGLVHISEVSHRRVGNINEELSQGQEVEAKILEIDKERKRIS
                     LSIKETMEPPVFEEEDKKEAAPEEAAAEPEAEAAPEEAEAAPEEAVAEPEAEAAPEEA
                     VAEAETEAAPEETVAEVESEPEAEAAPEETVAEPEVKEETEEQA"
BASE COUNT          897 a          648 c          616 g          808 t
ORIGIN      
        1 tctttgatta tttctgtaag tgattcataa agatatttaa gacgcagggg catttcagtg
       61 ccggcacagg tccttatggc tccttggcag caaacactga aatgattgcc cttcatttct
      121 acgataccat agcccattat tgcgtatccg ggatcgatgc ccagtatttt catatgtgct
      181 cctttttgat tttcaaggtt tttcagcatt ttcaacaact tgaattatag catacaaaca
      241 tatgtttgtc tataatactt cttgaaatgt ttagtttgac aatggtataa ttattaaaga
      301 taatgcaaga attgttttat cgggaaggag tttgttatat gagatattcg agacaaaaca
      361 aagtcctgga gttgataaac actcaggaaa tagaaactca ggaaaaactc tgtcagctcc
      421 tgaaggatgc gggctatgac gtgactcagg ctactgtgtc ccgtgatatt aaagaacttc
      481 agcttatcaa gaccctttct gccagcggca attataaata tgcggccggc gtcaactttg
      541 acggacccat cacagacaga ttcgtaaaaa tattcaaaga aacaataaaa tctattgatt
      601 cagcgggaaa catcattgtt gtaaaaactc tctcaggctg cggccctgct gcaggagaag
      661 caatagattc actggcactt ccccatatag tagggtccgt tgccggtgat aatactatta
      721 tgatagtaat agacgatccc gggcatgcag atgaagttct gaaagaattc cgcaagttct
      781 tataataatt tttccaacaa aagaggtcat caatgataac acatctcagt atcaaaaact
      841 tcgcaataat aagcgatatc gacatagatt tttataaagg tctgaatata atgaccgggg
      901 aaaccggagc aggtaaatcc atcgtcattg aagcgatgaa tattgccctg ggcgggagag
      961 ccgacaggac ttttataagg accggctgcg ataaggctgt cgttcaactg gtagccagcc
     1021 atataaatac agaaactgtg gttaccagag agctttatgc cgaaggcaaa agcctgtgca
     1081 ggatcgatgg ggaaatagta tctctggctc agctaaacga atactgccag aagataacag
     1141 atgtgcacgg acagtatgca caccagtccc ttcttgaccc cgggaatcac atcacactga
     1201 tcgactcata tgagaaggag tctatcgtct caacaaaaaa aagagtatcc gagctctacg
     1261 atgatttcag tgaagtctca aaaagacttg ctgaaatcca ctcttcggta agagaagaaa
     1321 tgcgcaaaaa agactatatg gagtttgaac taaaggaaat caaaaaggca gctcttgtgc
     1381 ccggagaaga agaaaagtta aaggagcagc ttgatacact caagtacatg gaaaccgtca
     1441 gcaacaatat ggaaaaggca tatatgcttg cccgggaaga cgaaaattct cctttggaga
     1501 gccttggtgc catacaaaat atattaaaag agatcgcccc ccactccaaa gacgcatctg
     1561 aacttgaaaa tgatttccac gatatctact accgtcttga agacatatgc accaggctcc
     1621 gtcaggcaaa agagaaaatc atattctctt cttccgattt ggatgaactt tctgcccgtc
     1681 ttgatcttat cgaaaccatg aaaagaaaat acggagacga tgtggagcaa atactggatc
     1741 atgcatcaaa actggaaaaa gctctttcca taatcgagaa tctggatacg gacaccgcag
     1801 ctcttaacct tgaaaaaagc aggatcgaag atatgctgaa agatgaaaca agaaggctga
     1861 gcagtcttcg caaagctgca gccaataccc ttgaagaaaa aatcaacaaa gagcttcttg
     1921 atctgaactt tcaggatgcc caaataagta tagctataaa gcccctttca ggatatacgg
     1981 caaacggaac ggatgatgtg gaattcctga taaaaacaaa caagggatcc gactttaaac
     2041 ccctggcaaa aatagcttcc ggcggagaaa tgtcccggat catgctggcc ttcaaaacgg
     2101 ttatcggaga ttatgatgat atcccaacca tggtttttga tgagatagac agtggtataa
     2161 gcggagcaac agccagtgta gtaggcaaaa aattaatgga acttgccggg aagcatcaga
     2221 taatatcaat aacacatctt ccccagatag ctgcatgcgg cgatcacaat ttccgcatcg
     2281 aaaaaaaatc ctccgagacc gaaaccttca ctacaataaa acatctttcc gaaaaagaaa
     2341 aagtcaacga gatcgcacga cttctttcgg gatcaaatgt ttcggacctt tcggtgaaat
     2401 ctgcaaaaga acttatagaa ctttcccaat gattgtattt ttattatcag acgaccgggc
     2461 acggcgatga cacatttttc tcacaataaa aaaacacggc agatagttat ctgccgtgta
     2521 tgtttttatt atgcctgttc ttctgtctct tcttttactt caggttcagc tactgtttct
     2581 tccggggctg cttccgcttc gggttcggat tcaacttcgg ctactgtttc ttccggagct
     2641 gcttcagttt cagcttctgc tactgcttct tccggggctg cttcagcttc gggttctgct
     2701 accgcttctt ccggtgctgc ttcagcttct tccggggctg cctcagcttc gggttctgct
     2761 gctgcttctt ccggagctgc ttcttttttg tcttcttcct caaataccgg aggctccatt
     2821 gtttctttta tgctgagact tatccttttt ctttctttat cgatttcaag gatctttgcc
     2881 tcgacttcct gtccctgaga aagttcttcg tttatgtttc ccactctcct gtggcttact
     2941 tccgaaatgt gcaccagtcc gtcaagacc
//
LOCUS       DAONHK010000129         2959 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_27530_length_2959_cov_3.964876, whole genome shotgun sequence.
ACCESSION   DAONHK010000129 DAONHK010000000
VERSION     DAONHK010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2959)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2959)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2959
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27530_length_2959_cov_3.964876"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="PLM92_05055"
     CDS             complement(<1..235)
                     /locus_tag="PLM92_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPR24539.1"
                     /translation="MDTSLYISNTKMQFVCGEEKKGGISVSAFKTVELPEETVINGLI
                     VNEGRFDEAFQKIIEAHPAPGGMKKVRLTIGSGQ"
     gene            complement(257..1462)
                     /locus_tag="PLM92_05060"
     CDS             complement(257..1462)
                     /locus_tag="PLM92_05060"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HPR24540.1"
                     /translation="MPLYEYSAAKINGRSIKGKKQAGNFAELRAQLEEDGLYLVNYKE
                     ADEGEKGKQLKAKQLSEFCRELGMMIGSGVPLIKALNVMIQRDVHPKMKKVYTRLHRG
                     LQRGLMLSEAMEEQGKTFPELLINMYKASEASGGMEETSRRMAQHYEKAYKLTQKVRS
                     AMIYPIILVIVTFIVLMVVFLLLLPKFFTLFEQFDSQIPAITKFMINLSHGLQDNWLY
                     VLIGVLMVIVAFRAFMQLEKVKAKVHRIYLKIPKIGKLLRTIYTARFARTLCSCYSSG
                     ISMINALENTRDTVGNKYIASQFENMINDVRNGENLSAAIAKVSGLDPKLAASVMIGE
                     ETGRLDQMLNDTADSFDFEADIALQKLTATAEPLLIVVMAVVIGSIMISVILPLPTLY
                     NSVGGAGGM"
     gene            complement(1484..2254)
                     /locus_tag="PLM92_05065"
     CDS             complement(1484..2254)
                     /locus_tag="PLM92_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008789574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HPR24541.1"
                     /translation="MIWVAYAVIYGLIFLAGICIGSFLNVLIYRMPLGINIAKGRSFC
                     PKCKNKLQAKDMVPLISFFVLRGSCRFCGQKISGRYPAVELMGGMLAVASLYFFWLTW
                     QALLVYAAVCILLVIAFIDIDTREIPDRLNIALFILALISVFLFPDIAVIERIIGFFE
                     ISLPMLILNFIIKDSFGGGDIKLCAACGFLLGYKAMLVGAFLGILTGGIYAIWLLISG
                     SKSRKDHFAFGPFLSFGLMVALYAGTMIWERYLEIFMF"
     gene            complement(2354..2743)
                     /locus_tag="PLM92_05070"
     CDS             complement(2354..2743)
                     /locus_tag="PLM92_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPR24542.1"
                     /translation="MYNSLMKRKNSVMKKNRKGFTLVEVIVVLVILAILAAILIPSMV
                     GWIDKANEKTVVAEARAALLACQTLASETYGEEGTASVDAGEAATLADVDGTISGVTL
                     DGAKVTAMVYANDDYTATWDGSAWSVE"
BASE COUNT          785 a          741 c          562 g          871 t
ORIGIN      
        1 cttgtccgct gcctatggta agccttactt ttttcattcc ccccggcgcc ggatgtgcct
       61 ctatgatctt ctgaaaagct tcatcaaatc ttccctcgtt gactatcaaa ccgtttatta
      121 cggtctcttc gggcagttca acagttttga aggcgctgac ggatataccg cctttttttt
      181 cttctccgca gacaaactgc atttttgtat tggaaatata tagtgatgta tccattggtc
      241 acctcataaa tatttattac attccgccgg caccacccac tgaattgtac agtgtgggta
      301 gcggaagtat caccgatatc attatactgc ctattacaac agccattaca acgatcagaa
      361 gcggttccgc tgtggcagtc agtttctgca gtgctatatc cgcctcaaaa tcaaaggaat
      421 ctgccgtatc gttcagcatc tggtcaagcc ttcctgtctc ctctcctatc ataacgctgg
      481 ccgccagttt aggatccagt ccgctgactt ttgctatggc tgcagaaaga ttttctccgt
      541 ttcgtacatc atttatcata ttttcgaact gggatgcgat atatttgttg cccactgtat
      601 ctctggtgtt ctcaagggca tttatcatcg atatgccact ggaataacag gaacaaagtg
      661 tcctcgcaaa tctggcggta tatatagtcc tgagcaactt gcctatcttg ggtattttca
      721 ggtatatcct gtgtaccttt gcctttacct tttccagctg cataaaagct ctgaatgcaa
      781 cgatcaccat cagcacgccg atgagaacat aaagccagtt atcctgcagt ccgtgactca
      841 ggtttatcat gaactttgtt attgccggta tctgggagtc aaactgttca aaaagcgtga
      901 agaacttggg gagcagaaga aggaaaacga ccataagtac tataaatgtc actatgacca
      961 gaataatcgg gtatatcatg gctgagcgga ccttttgcgt cagcttgtat gctttttcgt
     1021 aatgctgggc catcctgcgg gaggtttcct ccatccctcc gctggcttca ctggccttat
     1081 acatattgat cagcagttcc ggaaaggttt tcccctgttc ttccattgcc tcggaaagca
     1141 taagtcctct ttgcagtccc cggtgcagcc tcgtatatac ttttttcatc ttcgggtgca
     1201 catctctttg tatcattaca ttcaaagctt tgatgagagg gacaccggat cctatcatca
     1261 ttcccagctc acggcaaaac tcggaaagct gttttgcctt tagctgcttt cccttttctc
     1321 cctcatctgc ttctttataa ttgacgagat aaagcccgtc ttcttccagc tgcgctctga
     1381 gttcggcaaa attaccggcc tgtttcttgc cctttatact tctgccgtta atttttgctg
     1441 cactgtattc ataaagtggc acttaaaacc tcccattaaa aaatcaaaac ataaaaattt
     1501 caagatagcg ctcccatatc attgtgcctg catagagagc caccatcaaa ccaaaactca
     1561 gaaaaggtcc gaaagcgaaa tgatctttcc tgctttttga cccggaaatc aaaagccata
     1621 ttgcatatat gcctcctgta agtatcccca ggaatgcgcc caccagcatg gctttatatc
     1681 caagtaaaaa gccgcaggca gcacataatt ttatatcgcc tcctccgaaa ctgtccttga
     1741 tgataaagtt caggatcagc atggggaggg aaatttcaaa aaatccgatg atcctttcaa
     1801 taactgcgat atctgggaaa agaaaaacgg aaatcagagc cagaataaac agggcaatgt
     1861 taagtctgtc gggtatctcc ctggtatcta tatcaataaa tgcgatcacc aaaagtatac
     1921 agaccgcagc ataaacaaga agtgcctgcc atgtaagcca gaaaaagtaa agagaagcga
     1981 cagcaagcat gcctcccatc agttcaactg ccggataacg cccggatatt ttttgtccac
     2041 aaaaacgaca agaacccctc agcacaaaaa agctaataag gggcaccatg tcttttgcct
     2101 gtaatttatt tttgcatttg gggcaaaagg aacggccttt cgctatattt attccaaggg
     2161 gcatgcgata tatcaagaca ttaaggaaac ttccgatgca gatccctgcc aggaatatca
     2221 gcccgtatat tactgcatac gccacccaga tcatatttca gttttccccc aatagattca
     2281 gccctgcaat ttggtcgttt tgaaaagggc cgaagcagaa aactcctccg gccctgtcca
     2341 gttgtggtta gtcttactca acactccatg cgctgccatc ccatgtagcc gtgtagtcat
     2401 cgtttgcata taccatggct gtaactttcg caccatcaag ggtaacccct gatatggttc
     2461 cgtcaacatc tgctaatgtt gccgcttcgc cagcgtcaac tgatgcagtt ccttcttcac
     2521 catatgtttc cgatgccagt gtctgacatg ccagcaatgc agcccttgct tcagctacga
     2581 ctgtcttctc gtttgccttg tctatccagc ctaccattga cggaatcaag atcgccgcca
     2641 gtatagccag aatgacaaga accacgataa cctcgaccag cgtgaaacct ttgcggttct
     2701 ttttcatcac actgttcttt ctcttcatta atgagttata catttacttc cctcctgttt
     2761 tcccaatacg gtttattgta ttgttatttt agcatactcc ccctaaatta caactatttt
     2821 ttgtattttt ttgtaaaaaa gaggaagatt tgggtgaata ttctgtcatc caaaaaccat
     2881 ttacacaata tatagtgtcc aggatcagca attcacctaa aatcaggaaa cttcttcttc
     2941 gctatttggc gatcaagga
//
LOCUS       DAONHK010000130         2941 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_27814_length_2941_cov_3.518365, whole genome shotgun sequence.
ACCESSION   DAONHK010000130 DAONHK010000000
VERSION     DAONHK010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2941)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2941)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2941
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27814_length_2941_cov_3.518365"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(21..1331)
                     /locus_tag="PLM92_05075"
     CDS             complement(21..1331)
                     /locus_tag="PLM92_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011868623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HPR24543.1"
                     /translation="MIWNKTAECAERKQLEKLQLTKLKRTVERVYKNVETYRDKMQQK
                     GIAPGDITSLEDLKKLPFTTKDDLRDNYPYGMFASPLSEIVRIHASSGTTGKPTVVGY
                     TRRDLENWAECIARCFAMAGGDKSSVVQVAYGYGLFTGGLGAHGGAEYMGASVIPMSS
                     GNTQKQILLMKDFKSTILCCTPSYALTIAEALKKAGIATDELALESGIFGAEPWTSGM
                     KKEIEKGLGIKAYDIYGLSEVMGPGVACSCSEDKGMHIMEDLFIAEIIDPKTEEVLPY
                     GEKGEVVFTTIGKEGIPLIRYRTRDICALMPEKCGCGRTTLRMSRVMGRSDDMLIIRG
                     VNVFPSQIESVISGFDELTLNYKIIVGRKDNKDTFELHVELAEGLAIDDIRSVEELRK
                     RVDHDLRAVLGIGCTLRFLNEGTLPRSEGKAVRVEDTRNLSERV"
     gene            complement(1328..1924)
                     /locus_tag="PLM92_05080"
     CDS             complement(1328..1924)
                     /locus_tag="PLM92_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003533839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HPR24544.1"
                     /translation="MNSKEIKDLDILIVGVGGQGTLLASVILGNIALEGGYDVKLSEV
                     HGMAQRGGSVVTHVRISREPVKAPVIDKGGADIILAFELLEGFRWVPFLKKEGYMFVN
                     DSRINPMPVIMGMESYPEGIKEFFEEKKDRVTLIDALGIAEACGSEKAVNTVLLGAMS
                     SVLPFPEKKWIEQIEKKVKPAFIELNKKAFKAGREGNK"
     gene            complement(1927..>2941)
                     /locus_tag="PLM92_05085"
     CDS             complement(1927..>2941)
                     /locus_tag="PLM92_05085"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPR24545.1"
                     /translation="YQYAKEALPEASFLKLGAVNPLPKDLIKQFAEKVKKIFVIEEGQ
                     PFIEEQIKAMGINVCGKDLFTVQGEYSVNMIKEVFRKPLADHEKAGEAPVRPPLFCAG
                     CPHRALFYLLGKMKKTVTGDIGCYTLGSLPPTSSMDTCLCMGASIGMAHGFEKARGEE
                     FGKDVVAVIGDSTFFHSGITGLINMVYNKSKGTVLILDNRTTGMTGHQDNPATGKDIK
                     GNVSPEISIEELCRACGVQHIKEVDPFDLEEMEEAIKEECERDALSVIVSKRPCALIV
                     KQPGIPYVITEECKKCGICMGIGCPAIEKKGDDIVIITERCVGCSLCSRICPFKAIVK
                     EDR"
BASE COUNT          651 a          780 c          551 g          959 t
ORIGIN      
        1 attctcctcc tctgctaaaa ctatactctt tcagaaagat ttcttgtgtc ttcaactctg
       61 acagctttac cttcgctccg tggcagagtc ccttcattca ggaacctgag tgtacacccg
      121 atgcccagta cggcacgaag atcatgatcc actctctttc taagttcctc aacggatctt
      181 atgtcatcta tggcaagacc ttccgccagt tccacatgaa gttcaaatgt atctttgttg
      241 tcttttctgc cgactattat cttgtagttc agagtcagtt catcaaatcc gcttatgacg
      301 ctttcgatct gggacgggaa tacattgact cctcttatta tcagcatgtc atcagaacgc
      361 cccatgacac ggctcattct taatgtcgtt cttccgcatc cgcatttttc cggcataaga
      421 gcgcatatgt ctcttgtcct gtatcttata aggggtatgc cttctttgcc tattgtcgtg
      481 aatactactt cacctttttc tccgtaagga aggacttctt ccgttttggg atctattatt
      541 tcggcaatga acagatcttc cattatgtgc attcccttat cctcgctaca gctgcatgca
      601 actcccgggc ccattacttc cgaaagtccg tatatatcat aagcttttat tccaagtccc
      661 ttttctattt cttttttcat gcccgatgtc cacggctccg ctccgaatat gcccgactca
      721 agggccagct catcggtggc tataccggct tttttgagag cttctgcgat agtcagtgcg
      781 tatgaaggag tacagcaaag tatagtgctt ttgaaatcct tcatcagcag tatctgtttc
      841 tgtgtgttcc cactgctcat gggtatcacg gatgctccca tgtactcggc tcccccgtgg
      901 gctcccagcc ctcctgtgaa aagtccgtat ccgtaggcca cctgtaccac ggaggattta
      961 tctcctcctg ccattgcaaa gcatctggct atgcactcgg cccagttctc aaggtctctt
     1021 cttgtatatc ccaccactgt gggcttaccc gttgttccag atgatgcatg gatccttact
     1081 atctccgaaa ggggcgaagc aaacataccg tagggataat tatcccgcag atcatccttt
     1141 gtggtaaatg gcagtttctt aaggtcctca agagaagtta tatctcccgg agcaattcct
     1201 ttttgctgca tcttgtccct ataggtttca acattcttat agaccctctc cactgtccgt
     1261 ttcaattttg tgagttgtaa tttttccagt tgttttcttt cagcgcattc cgctgttttg
     1321 ttccatatca tttgtttccc tcccgtcccg ctttgaaggc ttttttgttt agctcaataa
     1381 acgcgggctt tacttttttt tctatttgtt ctatccattt tttttccggg aaaggaagta
     1441 ctgaggacat ggctcccaga aggactgtgt ttacggcttt ttcactgccg caggcttcag
     1501 cgatcccgag agcatcgatc aaagttactc tatccttttt ttcttcgaaa aactctttga
     1561 tgccctccgg atatgactcc atacccatta tgaccggcat tgggtttatg cggctgtcat
     1621 ttacaaacat atatccttct tttttcaaga aaggtaccca tctgaaacct tcaagcaatt
     1681 caaaggccag tataatatcg gctccaccct tatctattac aggtgccttg accggctctc
     1741 tgcttatcct tacatgggtc accacgctgc cgcctctctg ggccattccg tggacttcag
     1801 aaagctttac atcatagcct ccttcaaggg caatattccc cagtatcaca ctggctaaaa
     1861 gggttccctg accgccaacg cctacaataa ggatgtcgag gtcttttatt tctttgctgt
     1921 tcatccctat ctgtcctcct ttactattgc tttgaagggg catatcctgc tgcaaagact
     1981 gcagccgaca catctttcgg ttatgataac aatatcatca cctttttttt ctatggccgg
     2041 gcatccgatc cccatgcata tgccgcattt cttacattct tctgttatca cataaggtat
     2101 ccccggctgc tttactatca gtgcacaggg cctctttgaa actatcacag aaagagcatc
     2161 tctttcgcac tcttctttta tggcctcttc catttcttca agatcaaagg ggtccacctc
     2221 ttttatgtgc tgtactccgc aggctctgca aagttcctca atggatatct ccggggaaac
     2281 attgcctttt atatctttgc ctgttgcggg gttatcctga tgcccggtca ttcctgttgt
     2341 tctgttatca agtataagta cagttccctt tgacttgttg taaaccatat ttataaggcc
     2401 ggtgataccc gaatgaaaaa atgtggaatc gcctattaca gccactacat ccttcccgaa
     2461 ctcttcgcct ctcgcctttt caaatccgtg ggccatccct attgaagctc ccatgcaaag
     2521 acatgtatcc atggaagatg tagggggaag tgaacccagc gtatagcatc cgatatcacc
     2581 cgtaactgtc tttttcattt tacctaaaag ataaaacagt gctctgtggg ggcatcccgc
     2641 acaaaaaagc ggtggtctta caggtgcctc tcctgccttc tcatgatcgg caagaggctt
     2701 cctaaagact tcttttatca tgtttacaga gtattcaccc tgaacagtaa aaagatcctt
     2761 gccgcataca tttattccca tggcctttat ctgctcttca ataaagggtt gcccctcttc
     2821 tattacaaaa atctttttga ctttttctgc aaattgcttt ataagatcct tcggcagcgg
     2881 gttgaccgct cccagtttca ggaatgatgc ttcgggcaaa gcttcttttg catactgata
     2941 g
//
LOCUS       DAONHK010000131         2929 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_27970_length_2929_cov_3.949200, whole genome shotgun sequence.
ACCESSION   DAONHK010000131 DAONHK010000000
VERSION     DAONHK010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27970_length_2929_cov_3.949200"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="PLM92_05090"
     CDS             complement(<1..280)
                     /locus_tag="PLM92_05090"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPR24546.1"
                     /translation="MLSRISIKKPFTVLVAVILIIVFGVVAYMKMTPDLFPKIDLPYV
                     IVMTSYQGSTPEESEAEVTSPMEQQMASLEDLKNIQSVSGEGYSMIMLE"
     gene            complement(335..419)
                     /locus_tag="PLM92_05095"
     tRNA            complement(335..419)
                     /locus_tag="PLM92_05095"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(383..385),aa:Leu,seq:gag)
     gene            complement(436..2088)
                     /locus_tag="PLM92_05100"
     CDS             complement(436..2088)
                     /locus_tag="PLM92_05100"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPR24547.1"
                     /translation="MNISTVEIKKCFQKRKWLKYALITFVVLAILASLFVFKNDFFPG
                     DDEKEYRISSEMLFPMGRIKTLNPAASQDEDTYYITKLIYEGLFELDNTLTPEPLLAD
                     SYEFSKGKGSVDIKLKSGVKWHDGKDFTADDVKFTITALKTAGSKCLYAEQVSNISGT
                     SVKDDDEIVIYFRDKNNVSLDQLTFPVLPEHKYNDIYYSETREDNFKPVGTGPYEYKK
                     FSEAAYLDLGAYEDYHGQKAQNSIKFIVLSNRDNANRLVESSNISVLFSKEAGRAAKI
                     SKKNIETVDFPANEVEYIGYNMESASTSKKSVRKAIAYAIDTKTIIEECYYNSGLQND
                     NLYFPGYLGIKSNKDAYPYSTGKAAALLKSAGYKDRDNDGYLESADGTRLTINIAVDS
                     GNASRLSAAEYIKDFLDNLRIDTYIIEGDWNAYIARLNSRSYDIFIGGLKYTESMDMK
                     IILSGEGQSNYTGYKNGKMDDMLDEMRSGLSIEEMTSLYDEIKKLSKEELPYYCLLYK
                     TYGAIKAPALQGEVTPLFCDFYYGCERWKCRYEVTEDTSGEQ"
     gene            complement(2085..2675)
                     /gene="coaE"
                     /locus_tag="PLM92_05105"
     CDS             complement(2085..2675)
                     /gene="coaE"
                     /locus_tag="PLM92_05105"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HPR24548.1"
                     /translation="MKKVGLTGGIGSGKSTVSAYIEKKGIPVIDADKIAREIVKKGSP
                     VLTELSEAFGDDIIDEKGQLDRKKLASRAFSDIESKNILDRITQERIYDIIYSRLKNI
                     SLEGKGKMVLIDAPLLFESGLSDLADEIWVVSADLKTRVERVAERDMISREQISRIAQ
                     NQLDEEEKEKKADHVLDNSGTKEQLYEQIERLLKKI"
     gene            complement(2672..>2929)
                     /locus_tag="PLM92_05110"
     CDS             complement(2672..>2929)
                     /locus_tag="PLM92_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019314525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HPR24549.1"
                     /translation="MNSPIQGSAADIIKLAMVKVHEALKKGGYRSRLILQVHDELIVE
                     ALKEEQEAVSKLLKENMESAMELNVPLFVELNTGKNWYELK"
BASE COUNT          721 a          678 c          505 g         1025 t
ORIGIN      
        1 attccagcat tatcatcgag tatccctctc ccgatactga ctgtatgttt ttcaagtctt
       61 ccagcgaagc catttgctgt tccattgggg atgtaacttc agcttcggat tcttcaggag
      121 tggaaccttg atatgatgtc attacaatta catagggaag atctattttg ggaaaaagat
      181 ccggtgtcat tttcatatat gcaacaacac cgaatactat tatcagtatt acagcaacaa
      241 gaacagtaaa aggttttttt atactaattc tggacaacat ttatttcaga tccttccttt
      301 ttaaaaaccc gccggactcc cagcgggttt aagatggtgc gcccgaccag acttgaactg
      361 gtacgatgtt actcacacgc ccctcaaacg tgcgcgtctg ccgattccgc cacgggcgca
      421 taccgtgaat gctatttatt gttctccact tgtatcctca gttacctcgt atctgcattt
      481 ccatctttca catccataat agaaatcaca gaacaaagga gttacttctc cctgaagagc
      541 cggtgctttt atggccccgt aggttttgta aagaagacaa taatagggca gttcttcttt
      601 tgaaagcttc tttatctcat cgtaaagaga tgtcatttct tcgatggaaa gcccggatct
      661 catttcatca agcatatcat ccattttgcc gtttttatag cctgtataat tactttgacc
      721 ttccccggac aaaatgattt tcatgtccat gctctctgta tatttgagcc ctccgataaa
      781 aatatcataa ctccgggaat tcagcctggc aatataagca ttccaatcac cttctataat
      841 ataagtatca atccttaaat tgtcaagaaa atcttttata tattccgctg cggaaagcct
      901 ggatgcgttg cctgaatcaa cagcaatatt tattgtaagt ctggtcccat cagcactttc
      961 cagatacccg tcattgtctc tgtccttata cccggcactt ttcaaaagag ctgcagcttt
     1021 gcctgttgaa tagggatatg cgtctttatt gctttttata cccagatatc cggggaaata
     1081 cagattgtca ttttgaagac cgctgttgta atagcactcc tcgattatgg tttttgtatc
     1141 aatggcatat gcgatggcct tccttacact ttttttggat gtacttgctg actccatgtt
     1201 atatccaatg tattcaactt catttgcagg aaaatcaact gtttctatgt tctttttgga
     1261 tattttcgcc gctctcccgg cttcctttga gaaaagcaca gaaatattac ttgattccac
     1321 cagcctattg gcattatctc tgtttgacag aactatgaat tttatgctgt tttgtgcttt
     1381 ttgcccatga tagtcctcat atgcgccaag gtccagatat gcagcttcac tgaatttttt
     1441 gtattcgtag gggcctgttc ccacaggttt gaaattatct tcccgggttt ctgaataata
     1501 tatatcatta tatttgtgct ctggcagcac aggaaaagtg agctgatcca gagaaacatt
     1561 atttttgtct ctgaagtata ttacgatctc atcatcatct ttcaccgatg tgccggatat
     1621 atttgagacc tgttcggcat aaaggcattt actgccggca gtctttaggg ccgtaatggt
     1681 aaacttgaca tcgtcagcag tgaaatcttt gccgtcatgc catttcacac ctgatttcaa
     1741 ttttatatca acggatcctt ttcctttact gaactcataa ctgtccgcca gcagtggttc
     1801 cggcgtcagg gtattatcaa gttcaaaaag tccttcatat ataagctttg ttatataata
     1861 tgtgtcctcg tcctgcgatg cagccggatt taaagttttt atcctcccca ttggaaaaag
     1921 catctccgat gatattctgt attccttttc atcatctccc ggaaaaaaat cattcttaaa
     1981 aacaaataaa gatgccagga tagccaatac cacaaaagta atgagagcgt actttaacca
     2041 ttttcttttc tgaaaacact tttttatttc aactgtactg atgttcatat tttttttagt
     2101 agcctttcta tttgttcata aagctgttct ttggttcccg aattgtcaag aacatggtct
     2161 gctttctttt ctttttcttc ttcgtccagc tgattttgtg ctattctgga aatctgttcc
     2221 ctgcttatca tatctctttc ggcgactctc tcgactcttg ttttcaggtc cgcagaaacc
     2281 acccatattt catccgccag atctgaaagt cccgactcaa acagcagcgg ggcatctata
     2341 agtaccattt tccccttccc ctcaagggat atgtttttca gtctgctgta tataatatca
     2401 tatatcctct cttgtgttat tctgtccagg atatttttgc tttcaatatc cgaaaaagcc
     2461 ctcgaagcaa gtttttttct gtccagctgt cctttttcat cgattatgtc atctccaaaa
     2521 gcttccgaaa gttctgtaag caccggagat ccctttttta ctatttctct ggctatctta
     2581 tcggcatcga tgaccggtat cccctttttt tctatataag cagataccgt acttttgccg
     2641 cttcctattc ctcctgtcaa tccgaccttt ttcattttag ttcataccag ttctttcctg
     2701 tgttcagttc gacaaaaaga gggacattta gctccatggc gctttccata ttttccttca
     2761 agagttttga aactgcttcc tgttcttctt tcagggcctc cactataagt tcatcgtgca
     2821 cctgcagtat caatctggat ctgtatccgc cttttttgag tgcttcatga acttttacca
     2881 tggccagttt tattatatcg gctgcgctgc cctgtatggg gctgttcat
//
LOCUS       DAONHK010000132         2926 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_28028_length_2926_cov_2.447928, whole genome shotgun sequence.
ACCESSION   DAONHK010000132 DAONHK010000000
VERSION     DAONHK010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2926
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28028_length_2926_cov_2.447928"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="PLM92_05115"
     CDS             complement(<1..468)
                     /locus_tag="PLM92_05115"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011968596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HPR24550.1"
                     /translation="MKKTVIEIKKLNEEAMEEAAKRQEKLAKPPGSLGRLEEISIRLA
                     GITGSPISRIEKPCIIIMCADNGVIAEGVASAPLSVTMAQTINFTMKKTGVGALAKEF
                     GTDLMVIDVGINGKVPEGLKTTDYPGPGKEVKIIDRKIAMGTNNINEGPAMTLS"
     gene            complement(602..1246)
                     /locus_tag="PLM92_05120"
     CDS             complement(602..1246)
                     /locus_tag="PLM92_05120"
                     /inference="COORDINATES: protein motif:HMM:TIGR02370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase cognate corrinoid protein"
                     /protein_id="HPR24551.1"
                     /translation="MEQLFEEMKNAVLEGEEEEAAELAQKALDSGIDPVAAMDNGFLK
                     GINEAGRLYEEEEFFLPELVCAADAMKAALGVLDEELKKCADNNTKTGKVVLATVQGD
                     VHDIGKTIVGAMMTANGYEVYDLGADVPNEKVVSSIDEIQPDIIGLSALLTTTMENQK
                     NVIEMISEAGKRDRVKIMVGGAPVSRQWSEKIGADGYSDNAIDAVKMAGTLIGK"
     gene            complement(1261..2793)
                     /locus_tag="PLM92_05125"
     CDS             complement(1261..2793)
                     /locus_tag="PLM92_05125"
                     /inference="COORDINATES: protein motif:HMM:NF018012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HPR24552.1"
                     /translation="MKEVQSIKEVQSKKKNLEHKRSSDQSRKEKKMLMQSKVLSDEEI
                     RTIHKNSIRILEETGVKFPSEKALDLLEDAGAEIDREKEIAYINEDMVKKALQTAPKE
                     FTLGAPDPKYDLSLPAAFPTHNIDGCGMYVLDHGSRKKRPGTTKDIENSFRIFQTMDQ
                     GSIGWAPIVAADLPPKAAGVTETGLFYINSSKHYQDEETSMKGVGYHMEMAKAILGSW
                     EEVRKRKIYSATYCTVAPLCHDHTMLEATMELTKYEAPILAYPMPACGSTGPASLFTN
                     VAMANAEALSCFVIFQLCNPGTPIIYGAALGSINMKSGIFLEGAPETALQLTAMTQMG
                     RYYGFPTEVAGCLSDSKEPGMQAILEKYMTMEPLIAASVDVVQGIGLIESSMTLSPEQ
                     MIIDDELFRYAVRIRRGIDPSEEKDLLEDIKAVGPEGHFLKQKSTRKLFRSDEFYRPG
                     NLIDRNSHDEWISLGSPDMYSVAHKRVVDILSEEPAGFLDRDTEKVIREIMEEAKAKM
                     SS"
BASE COUNT          592 a          771 c          568 g          995 t
ORIGIN      
        1 gctcagggtc atagccggcc cctcgtttat attattcgtt cccatggcta tttttctgtc
       61 tattatttta acttcttttc ccggcccggg ataatccgta gtcttcaatc cttcaggcac
      121 cttgccgttt attcccacat cgatcaccat gagatccgtt ccgaactctt ttgccagggc
      181 tcctacccct gtctttttca ttgtaaaatt tatagtctgt gccattgtca cggataaagg
      241 tgccgatgcc acaccttccg caatgacccc gttatcggcg cacatgataa taatacaggg
      301 tttttctatt ctgcttatgg gacttcctgt aatacccgcc agccttatgc ttatctcttc
      361 cagtctgccc aggctgcccg gaggcttagc cagtttttcc tgccttttag cggcttcttc
      421 cattgcctct tcattcagtt tttttatttc tatcactgtt tttttcatac atttatagta
      481 tcacaaaaac agcatccgaa aacaccaaag ttcaaaagga tgctgatttt cgcgctcttt
      541 ttttcttttt ttgctctgca tttcatgcct gcttgatttg caggatcccg gtaaaagtcc
      601 tctactttcc tattagtgtt ccggccatct ttacggcatc gatagcattg tcggaataac
      661 cgtcagcgcc tatcttctcg ctccactgcc tgctgaccgg agctcctcct accattatct
      721 tgactctgtc tcttttgccc gcttcgctga tcatctcgat aacatttttc tgattttcca
      781 tggtggtggt gagcagggca gagagaccta ttatatcagg ctggatctca tcgatggagc
      841 ttaccacctt ttcgttgggc acatctgctc ccagatcata aacttcataa ccgttggcag
      901 tcatcatggc tcccactatg gtctttccta tatcatgcac atctccctga actgtggcaa
      961 gaaccacctt tccggtcttt gtgttattgt cagcgcactt cttcagttct tcatcaagga
     1021 ctcccagagc cgctttcatg gcatcggcag cacatacaag ttcaggcaaa aagaattctt
     1081 cttcttcata aagcctgccc gcctcattta tgccttttag aaatccattg tccatggcag
     1141 ccacaggatc gattccgctg tcaagggctt tttgcgccag ttcggcagcc tcttcttcct
     1201 cgccttccaa aaccgcattt ttcatttctt caaataactg ttccattttt tcctcctgta
     1261 tcaagaactc atttttgctt ttgcttcttc catgatctct cttattactt tttctgtatc
     1321 ccggtcaagg aacccggcgg gctcctcaga aagaatgtca actactcttt tatgagccac
     1381 cgaatacata tccggactgc ccagggagat ccattcatca tgagaatttc tgtctatcag
     1441 attcccgggc ctgtaaaact catcgctcct gaaaagtttt cttgtacttt tctgtttcag
     1501 gaaatggcct tcaggtccta cggcctttat atcttcaaga aggtcctttt cttctgacgg
     1561 atctatacct cttctaatac ggaccgcata acggaaaagt tcgtcatcta ttatcatctg
     1621 ttccggagaa agtgtcatgc tgctttcaat aagacctatg ccctgcacca catccacact
     1681 tgccgctata agaggctcca tggtcatgta tttttcaagt atcgcttgca tacccggttc
     1741 tttggaatcc gaaaggcatc ctgcgacttc agtgggaaat ccgtagtatc tgcccatctg
     1801 ggtcatcgcc gtaagctgca gcgccgtttc gggagctccc tccaggaata tgccgctttt
     1861 catgtttatg gatcccaatg ctgcgccgta gattatggga gtccccggat tgcagagttg
     1921 aaatatcaca aagcacgaca gggcttccgc gttggccatt gcaacatttg taaaaaggct
     1981 ggcgggtccg gtgcttccgc aggccggcat gggatatgcc agtatcggtg cctcatattt
     2041 ggtcagttcc atggtggctt ccagcattgt atggtcgtgg caaaggggtg caacagtaca
     2101 ataggtggcc gagtatatct ttcttttcct gacttcctcc cagcttccca gaatggcctt
     2161 tgccatttcc atatgatagc ctacgccctt catgctggtt tcctcgtcct ggtaatgctt
     2221 actgctgttt atataaaaaa gtccggtttc cgttacgccc gcagccttgg gaggcagatc
     2281 ggccgccacg atgggagccc atcctatact tccctgatcc atggtctgga atatcctgaa
     2341 actgttttct atgtcttttg tcgtccccgg tctttttttt ctgctgccgt gatccagaac
     2401 gtacataccg cagccgtcga tgttatgggt gggaaatgca gctggcaaac taagatcata
     2461 tttcggatcc ggtgctccca gcgtgaattc ttttggtgct gtttgaaggg ccttcttcac
     2521 catgtcttca tttatatatg ctatctcttt ttctctgtct atttctgctc cggcgtcttc
     2581 cagcagatcc agcgcctttt cgctggggaa tttgaccccg gtttcttcaa gtatccttat
     2641 gctgttttta tgtatggtcc tgatttcctc atcgctgagt acttttgact gcatcagcat
     2701 ttttttctcc tttctgctct gatctgaact tcttttatgc tctaagttct ttttcttgct
     2761 ctgaacttct tttatgctct gaacttcttt catgctctaa gttcttttct tgctctgaac
     2821 ttcttttata tattaaaccc tataatcaaa taataggtct ttctgcccgg gaataggggt
     2881 atatttttgt tgttatcagg gtatattttt gatatcattg atttat
//
LOCUS       DAONHK010000133         2924 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_28047_length_2924_cov_4.420007, whole genome shotgun sequence.
ACCESSION   DAONHK010000133 DAONHK010000000
VERSION     DAONHK010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2924
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28047_length_2924_cov_4.420007"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..826)
                     /locus_tag="PLM92_05130"
     CDS             complement(<1..826)
                     /locus_tag="PLM92_05130"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF012911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HPR24553.1"
                     /translation="MKKQTTEKIKLWGIVQGVGFRPYVARIAYDMGIKGRVRNIGGLV
                     EITVTDKKERITEFLDVLKKEKPVPAEIVHISRETLPLEFFGHFTIKQSDDGDKETAM
                     IPADLAVCPDCLREMNDEDDPRRGHPFISCMVCGPRYTITDRFPYDRDNTSMVDFDMC
                     RYCREEYKDIRNRRYHAQTISCYDCGPQMEMYPEDEENIQNDEDLTKRNRKILEYAAS
                     LLKKGKVIAFKSMGGYNLMADPKNQEATSALREIKAREEKPFAVLFRDEKQIGEYCI"
     gene            1036..2544
                     /locus_tag="PLM92_05135"
     CDS             1036..2544
                     /locus_tag="PLM92_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019417416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HPR24554.1"
                     /translation="MFLREQWNSKLGFLLAAIGSAVGLGNIWRFPYICAENGGGAFLV
                     PYFFAIITAGIPILILEYTIGKTYRAGAPTALARINKRFEWLGWAQVMVAFLITVYYF
                     AIVVWVVSYLGFSFTEAWGKDPTTFFISYLGLTESGTQLGGIQLNLVIPFLIVWVLAA
                     VILYAGIKKGIEIACKICLPILLVMVFVLVIRGLTLPGAVDGLEYMFKPNWGALTDSS
                     VWVAAYGQIFFSLSIAFAIMISYSSYLPKKTDVVNSAFITATANHGFEVFAGIGVFSI
                     LGYMALQQGVAVEEVAGAGVGLAFMTFPTAISTLPALNAFFGVCFFGALFTAGITSLI
                     SIIQAVVTGIMDKFDMSHKKAVTMVIVPAFIVSLLFITGAGLTILDIVDAFVNNIGVA
                     GCGLLEVLLIGWFFSPEKLREAANEYSNFSVGKWWTYCLKIVTVIVLGVMLVLNIKTY
                     ITEGYGGYAQLDVTLYGWGAVLIIVLGSIIFTSMKGKDGYRDLSKIAEKEVN"
     gene            2546..2662
                     /locus_tag="PLM92_05140"
     CDS             2546..2662
                     /locus_tag="PLM92_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016958249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetS family NSS transporter small subunit"
                     /protein_id="HPR24555.1"
                     /translation="MSASAIIMMIIGCGALWGGCATAVIINMRHSKKEKQQQ"
BASE COUNT          760 a          614 c          615 g          935 t
ORIGIN      
        1 ttatgcagta ttctccgatc tgcttttcat ccctgaacaa aacagcaaag ggtttctctt
       61 cccttgcttt tatttctctt aaggctgatg ttgcttcctg atttttcgga tctgccataa
      121 gattgtatcc acccatgctt ttgaatgcaa tgacttttcc ttttttcaga agagatgccg
      181 catattccaa tatttttctg tttctttttg taagatcttc gtcattttgt atattctctt
      241 catcttcggg atacatttcc atctggggac cgcagtcata gcaggatatt gtctgggcat
      301 ggtatcttct gtttcttata tccttatatt cttcccggca gtacctgcac atatcaaaat
      361 ccaccatgga agtgttgtcc ctgtcatagg gaaatctgtc agttattgtg tatctgggcc
      421 cgcaaaccat acagcttata aaaggatgcc ccctccttgg atcatcctca tcattcattt
      481 cccttaaaca atcgggacaa acagcaagat ctgccggtat catggcagtc tctttgtcgc
      541 cgtcatcact ttgttttatg gtaaaatgac cgaaaaattc aagaggcaga gtttctctgc
      601 ttatatgaac gatctcagcc ggtacgggct tttctttttt tagtacgtcc aggaattctg
      661 ttattcgctc ttttttgtcg gtaacagtta tttcgaccag tcccccaata ttgcggacac
      721 ggccttttat gcccatatcg taggctatcc tggccacata aggtctgaat ccaacgccct
      781 ggactatgcc ccataatttg attttttccg ttgtttgttt tttcatttta tcattcagcc
      841 acaaatattc aattaatatt ataattcctt tcagtagtat atacaattta tatttttgca
      901 caaagttgaa tataattttt tgttgtctta caatgaaaaa cgttgacttt tcaatatttt
      961 tttgatatat tattatccgt tggatttatt tttaattttt tggcacaaag tttgtgaatt
     1021 acatttagga ggtgcatttt tttgagagaa caatggaact ctaaactggg ctttttactg
     1081 gcagccatcg gttctgccgt aggccttggc aatatatggc gtttcccgta tatttgcgca
     1141 gaaaacggag gcggggcctt tttggttccc tacttcttcg ccataataac agcaggtatt
     1201 cctatactta ttctggaata caccataggc aaaacctacc gtgccggcgc tcccacggcc
     1261 ctggccagaa taaacaaaag attcgaatgg ctgggctggg cacaggtaat ggtggctttc
     1321 ctcataaccg tttattattt tgcaatagta gtatgggtgg taagctactt aggattttcg
     1381 tttacagaag catggggcaa agatcccact actttcttta taagctatct gggtcttaca
     1441 gaaagcggca cacaactggg aggaatacaa ttgaatcttg tgataccgtt ccttattgtg
     1501 tgggtgctgg ctgcggtcat actttatgca ggcataaaga aaggtattga aattgcatgc
     1561 aagatttgtc tgcctatact tcttgtaatg gtttttgtac tggttataag aggtttaact
     1621 cttcccggtg cagtagatgg tctggagtac atgttcaaac ctaactgggg agcccttacg
     1681 gattccagtg tgtgggtagc ggcatacgga cagatattct tcagtttgtc catagccttc
     1741 gctataatga tctcctactc cagttatctt ccaaaaaaga ccgacgttgt aaacagcgca
     1801 ttcataactg caacggcaaa tcacggtttc gaagtatttg caggcattgg cgtattttcc
     1861 atcttgggat atatggcctt gcagcagggt gttgccgtag aggaagttgc cggtgccggc
     1921 gttggtctgg cattcatgac attccccaca gccataagca cactgcctgc cctgaatgca
     1981 ttcttcggag tatgtttctt cggagctctt ttcacagccg gtattacttc actgatttcc
     2041 atcatacagg cagttgtaac aggtataatg gataaatttg atatgtcaca caaaaaggct
     2101 gttaccatgg tcattgttcc tgctttcatc gtaagccttc tgttcataac aggtgcaggt
     2161 cttacaatac ttgatatcgt cgatgcattc gtaaacaaca ttggcgttgc cggatgcggt
     2221 cttcttgaag tcctgcttat cggatggttc ttcagtccgg agaaactcag agaagctgcc
     2281 aacgagtatt ccaatttcag cgttggcaaa tggtggactt actgcctgaa aatcgttact
     2341 gtaatcgttc ttggtgttat gctcgtgctc aatatcaaaa cttatatcac cgaaggttac
     2401 ggcggatatg cccagctcga tgtaactctt tacggatggg gcgcggttct cataatagta
     2461 ctcggatcaa taatcttcac atccatgaaa ggcaaggacg gctacaggga tctcagcaag
     2521 atcgccgaaa aggaggtaaa ctgatatgag tgcaagtgca ataataatga tgataatagg
     2581 ctgtggagct ctttggggcg gctgtgcaac agctgtaata atcaacatga gacacagcaa
     2641 aaaagaaaaa caacagcaat aacattgaca aaaagagccg gtaaaccggc tctttcagac
     2701 tgtagacaaa agtccgacct cggaaacgag gcaacggact tttttctatt gcatgtgtta
     2761 aacaccgaac gaaagtgaat atcgttgaaa atttgtgaaa acccgcataa gtgaagaaaa
     2821 tagttgttta acaagggaac ctcccctata tgaagatgga ttgccgggct ttgcaggatt
     2881 gtgatctctg tatttccaaa gcatatttgc aagctttttc atgt
//
LOCUS       DAONHK010000134         2883 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_28627_length_2883_cov_4.413720, whole genome shotgun sequence.
ACCESSION   DAONHK010000134 DAONHK010000000
VERSION     DAONHK010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2883
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28627_length_2883_cov_4.413720"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..628)
                     /locus_tag="PLM92_05145"
     CDS             complement(<1..628)
                     /locus_tag="PLM92_05145"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HPR24556.1"
                     /translation="MRSIRNYIKSLVLIIIILALSFTQTVPVFGIDEEPETASRAAIL
                     MDAKTGEIIYEKNADRVMEPASITKVMTCMLAIENLDPDHKVTISRDMDLLGHVMSLK
                     KGEQLTVRQLLYSMMVWSANDAAVVLAEEVSGSVEEFAELSTKRAHELGAENTFYKNP
                     NGLNDVTGHVTTAEDLALICRWAMENRTFREIVSTVRYKVPATEVSEER"
     gene            complement(625..1836)
                     /locus_tag="PLM92_05150"
     CDS             complement(625..1836)
                     /locus_tag="PLM92_05150"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010247506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cofactor-independent phosphoglycerate mutase"
                     /protein_id="HPR24557.1"
                     /translation="MKYLILVPDGAGDEKIEELGGKTPLEEAHMPVIKKLAQLGETGM
                     VKTIPDGVPPGSDAANLSVMGYDPAVFLTGRSPLEAASIGIEMSDTDVAFRTNLVTLK
                     GEGNYEDLIISDHSAGDISTEEADQLIKAVNKEFADKNISFYTGVSYRHLMIVHNGST
                     EMELTPPHDQLGKRAGDHLPKGRGSEFIADMMKKSYDILDDHPVNRARKAKGLGPANS
                     IWIWGQGKKPSLSSFTEKYGITGTAISAVDLIKGIGICAGLSTVDVQGATGTINTNFE
                     GKARAAIEAFRDGKDFVYLHIEGPDECSHQGDMEGKIKCLEIIDEKVLKPILEYLESS
                     SEDFSILEVPDHRTPLSIRTHSSDPVPYVIYRSVEQKPLDESREFSERSGEKGRFFGS
                     GFALADYFFQK"
     gene            complement(1833..>2883)
                     /gene="serC"
                     /locus_tag="PLM92_05155"
     CDS             complement(1833..>2883)
                     /gene="serC"
                     /locus_tag="PLM92_05155"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009644111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HPR24558.1"
                     /translation="SMLPLEVIEEAAADLANYKGCGESVIEMSHRSGEFAAIISDAEA
                     DLREIMEIPQNYRVMFIQGGATLQFSMVPMNLMRKSKKADYLITGSWAKKAAKEAKKF
                     GDIKITASSEEDNFSYIPEVTRGDFREDADYVHITSNNTIYGTKFPYIPDTGDIPLVS
                     DMSSCILSEKIDVSRFDLIYAGAQKNIGPAGVTIVIAREDLLGFADKNIPVYLDYKTH
                     SDNGSTYNTPPCFAIYIAGQVFKKIKKDGGISVIEKKNKAKANKLYGFIDSSDFYKCP
                     TREKDRSIMNVVFVTGDEEKDKKFVSEAKALGMHNLNGHRSIGGMRASIYNAMPKEGV
                     DTLIEFMKKFEAENK"
BASE COUNT          665 a          720 c          583 g          915 t
ORIGIN      
        1 ttctttcttc agatacctct gtggcaggca ctttgtacct taccgtagag acaatttccc
       61 tgaaagtccg gttttccatt gcccaccggc atatcagcgc cagatcctct gcagttgtca
      121 catgccctgt cacatcatta agaccgttgg gatttttgta gaatgtgttt tctgcgccca
      181 gttcatgggc tcttttggtg gaaagttccg caaattcctc gacgcttccc gaaacctctt
      241 ccgccagaac aactgccgca tcatttgccg accacaccat catcgaataa agaagttgtc
      301 tgactgtcag ctgttctccc ttctttagag acattacatg cccaagaaga tccatatctc
      361 tgcttatagt gaccttatgg tccgggtcaa gattttctat ggccagcatg caggtcatta
      421 ctttggttat actggcaggc tccattaccc ggtccgcatt tttttcataa atgatttccc
      481 ctgttttggc atccatcagg atagctgcac gggaagccgt ttcaggttct tcatcaatac
      541 caaaaacagg cactgtctga gtaaaagaaa gtgccagaat gataattatc aaaaccaggc
      601 tttttatata atttctaatg cttctcattt ttgaaaaaaa taatctgcca aagcaaagcc
      661 gcttccgaaa aaccggccct tttctccgga tctctcggaa aattcccttg attcgtccaa
      721 tggcttttgt tcaacgctcc tgtaaatcac atagggcacc gggtctgaag agtgggtcct
      781 tatggacagg ggcgttctgt gatccggaac ttccagtatg ctgaaatcct ctgaactgct
      841 ttccagatat tccaatatgg gctttagtac cttttcatct atgatctcaa gacattttat
      901 cttgccttcc atatccccct gatgagagca ttcgtcagga ccttcaatgt gaagatatac
      961 aaaatccttt ccgtctctga aagcttctat ggccgcccgg gctttccctt caaaatttgt
     1021 gtttattgtg ccggtggcac cttggacatc cacagtggaa agtcccgcac atatgcctat
     1081 gcctttgata aggtccacag ccgatatggc tgtgcctgtt ataccgtatt tttctgtaaa
     1141 ggaagaaaga gagggcttct ttccctgacc ccatatccag atcgaattgg ccgggccaag
     1201 accctttgct tttctggctc tgtttaccgg atgatcatca agtatatcat aacttttctt
     1261 catcatatca gcgataaatt cgctgcccct gcctttggga agatgatctc cggctctttt
     1321 gcccagctga tcatggggag gagtcaattc catctccgta gaaccgttat gaactatcat
     1381 cagatgtctg tagcttactc cggtataaaa gcttatattt ttatctgcaa attctttatt
     1441 taccgccttt atgagctgat cggcttcttc cgttgatatg tctccagcac tgtgatcgga
     1501 aatgataaga tcttcgtagt tgccttcacc tttcagggtg accagatttg tccgaaaagc
     1561 cacatctgta tcagacattt ctatgcctat actggcagcc tccagaggag agcgacccgt
     1621 aagaaaaact gcggggtcat atcccatgac agacaggttt gccgcatcgc tgccgggagg
     1681 aacgccatcg ggtatggtct tcaccatacc tgtttctccc agctgcgcca gtttttttat
     1741 caccggcatg tgtgcctctt caaggggagt tttgcctccc agttcttcga ttttttcgtc
     1801 cccggctcca tcgggaacaa gaatcagata tttcatttgt tttctgcctc aaacttcttc
     1861 ataaactcaa taagtgtatc tactccttct ttcggcatcg cgttatatat gctggcacgc
     1921 atgccgccta tggagcggtg tccgttaaga ttatgcatac ccagagcctt tgcctccgaa
     1981 acgaatttct tgtctttttc ttcgtctccg gtgacaaaaa ccacattcat tatagacctg
     2041 tccttctccc tggtggggca tttgtagaaa tcactcgaat ctataaatcc gtacaactta
     2101 tttgcctttg ctttgttctt cttctcgatg acggatatcc ctccgtcctt ctttatcttt
     2161 ttgaaaacct gccctgcgat gtatatggca aagcatggcg gagtattata tgtggagccg
     2221 ttatctgaat gtgtcttgta atccagataa acaggaatgt ttttatctgc aaatcccaga
     2281 agatcttccc tggctatgac aatggtcacg cctgcaggtc ctatgttctt ctgtgctcct
     2341 gcataaataa ggtcgaatct gctgacatcg attttctccg aaagtatgca tgaagacatg
     2401 tcgctgacca gcgggatgtc tcctgtatcg ggtatgtagg ggaactttgt tccgtaaatc
     2461 gtgttgttgg aagttatgtg tacataatcc gcatcttccc tgaaatctcc ccttgtgact
     2521 tcgggtatgt atgaaaagtt atcctcttcg gaagatgctg ttattttgat atcgccgaac
     2581 ttctttgctt ccttggcagc cttttttgcc caggatcccg ttataagata gtccgctttt
     2641 tttgattttc tcataaggtt catggggacc atggaaaact gcagcgtcgc acctccctgg
     2701 atgaacatta ccctgtagtt ttgcggtatt tccattattt ccctgagatc tgcttccgca
     2761 tcagaaataa tggctgcaaa ttccccggaa cggtggctca tttctattac agattcaccg
     2821 catcctttat aattcgcaag atctgctgct gcttcttcta ttacttcaag cggaagcatg
     2881 gat
//
LOCUS       DAONHK010000135         2815 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_29659_length_2815_cov_4.508333, whole genome shotgun sequence.
ACCESSION   DAONHK010000135 DAONHK010000000
VERSION     DAONHK010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2815
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29659_length_2815_cov_4.508333"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1048
                     /locus_tag="PLM92_05160"
     CDS             <1..1048
                     /locus_tag="PLM92_05160"
                     /inference="COORDINATES: protein motif:HMM:NF012392.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HPR24559.1"
                     /translation="FAEMMLIDTSENLTHISQACGFSSPDYFNKCFKALHGKTPAQYR
                     KSFVQRARIYGKPLSEKKALELLKTYLPPSDNVFLNGISFEAVLHTDFMRTSGEDFSG
                     LFRRRLIIDSNDDLLKLKGLERELRYLNFSSVALSYDFMNGIKNGNSLKDAESYLDSF
                     GLPVEKLTSSLAPYMQTYSEKVRPADTAAMIFKTACPGVFLSGEKNSLFSQKAVATPY
                     YFTFCALADISGNVTVSQEQGMAIKGHESLFIILFNKDEKRSLKYRVNIKNLPEKCCM
                     VSKHFCGQDNQYDIPLDTGPLKIIPERLRVHLNSAFCGKTSFSSLTGANDMPVIFDLS
                     PGSLAVVEIIKTDI"
     gene            complement(1069..2736)
                     /locus_tag="PLM92_05165"
     CDS             complement(1069..2736)
                     /locus_tag="PLM92_05165"
                     /inference="COORDINATES: protein motif:HMM:NF024910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HPR24560.1"
                     /translation="MKENQAGSMTQQKTEGVAGLKKYDVRIGGVVFLIYCLVAAGAFG
                     IEEMIPEAGPGMTITLLCVFPIIWAWPISNICAEMGSVIPAEGGVYVWAQKAFGEFWG
                     FQAGWWNTVSIYVTNGVYVALVVGYARQFIPMPDATAFAIKVAMIAFFTIINLLGIRE
                     VSKASTVFSILIVGAFAVVAVVGFANWNYNPMEPFVPEDYGILDGIGGGICICVWMYC
                     GYECISTLAGEVKNPQVIPKGLLIAMPLIALTYVLPTVGGLASVGNWENWSTDGGFDS
                     SSVGYATVLTENLGQAWGYIFLFIAIISQCAIFNTYLASGSRGFFVMADDNLFPRTLV
                     KVSKKRGVPSVGILSLALVTLFLAQFDFATLVNTEVVFILALYMILPLCTIKLRKIYP
                     IEERRANGQYVMPGGKVGLYFFTMMPLIISIFALLINGTDYFLVGLVGISTGPLAYML
                     FKWVKGGLAKNDPVKYPLNQKTKLAAGDISRLGLFSFLTGIFAFLGQFWLRWYEIDYG
                     EWGPGDYDMFGDKVPQMLEILKWGGLVLIVLGIILFIVDKKIEPKKE"
BASE COUNT          776 a          693 c          551 g          795 t
ORIGIN      
        1 gtttgcagaa atgatgctta tcgacacatc tgaaaatctc acccacataa gtcaagcctg
       61 cggcttttct tctcccgatt acttcaacaa atgctttaag gccctccacg gtaaaacccc
      121 tgcccagtac aggaaatctt ttgttcaaag ggcacggata tacggaaaac cattatcaga
      181 aaaaaaagct cttgaacttc tcaagactta tctcccccct tcggacaatg tctttttgaa
      241 cggcatatcc tttgaagcgg ttttgcatac ggacttcatg cggacctccg gcgaggattt
      301 ttcgggactg ttcaggcgca gactcataat agactctaat gatgaccttc tgaaattaaa
      361 aggacttgaa cgggaacttc gatatctcaa cttttcttct gttgctttaa gttatgattt
      421 tatgaatggc atcaaaaatg gcaacagtct taaagatgcc gaatcctatc tcgattcttt
      481 cgggctccct gtggaaaagc tgacttcttc tctcgctccg tatatgcaga catacagcga
      541 aaaagtaaga cctgcggata ctgccgcaat gatctttaag acagcttgcc ccggggtttt
      601 cctttcagga gaaaagaatt cccttttctc ccaaaaagcc gtagctacac catattactt
      661 cacattttgt gcccttgctg atatttcagg caacgtgacg gtttcccagg aacagggaat
      721 ggcaataaag ggtcacgagt ctctttttat catccttttt aacaaagatg aaaaacgctc
      781 tctgaagtac agggtaaaca taaaaaacct gcccgaaaaa tgctgtatgg tttccaaaca
      841 tttttgcggg caggataatc agtatgatat acctttagat accggtccct taaaaatcat
      901 tcccgaacgc ttaagggttc atctgaactc cgccttctgc ggcaaaactt ccttttcttc
      961 attgaccggt gcaaatgaca tgcctgtgat ctttgacctt tctcccggaa gtcttgccgt
     1021 tgttgaaata ataaaaacgg atatttgatt tgccttttca gcagtttttt actctttctt
     1081 cggctctatt tttttgtcta cgataaaaag tattataccg agaactatca gaacaaggcc
     1141 tccccatttt aatatctcca gcatctgggg aactttatct ccgaacatat cataatctcc
     1201 gggcccccac tctccgtagt ctatctcata ccacctgagc cagaactgtc ccaggaatgc
     1261 aaatatgccg gtgaggaaag agaacaatcc aagcctggaa atgtctcctg ctgccagttt
     1321 tgttttctgg ttcagaggat atttcacggg gtcgtttttg gcaaggccgc cctttaccca
     1381 tttgaataac atatatgcca gaggtcctgt gcttataccg acaagtccca caagaaaata
     1441 gtctgttccg tttatgagca gtgcaaaaat cgatataata agaggcatca tggtgaaaaa
     1501 gtaaagtccc actttgcctc ccggcattac atactgccca ttggctcttc tttcttctat
     1561 ggggtatatc ttgcggagct ttattgtgca aaggggaagt atcatataaa gagccagtat
     1621 gaaaaccact tctgtgttaa cgagagtggc aaaatcaaac tgtgccagga acagtgtcac
     1681 taaggccagc gacaatatgc ccactgacgg cacacccctc tttttgctta cttttaccag
     1741 agtccttggg aatagattgt catcagccat tacaaagaat cctcttgatc ccgaggcaag
     1801 atatgtgttg aatatggcgc actgggaaat tatagctata aaaaggaata tatatcccca
     1861 tgcctgtccc agattttctg tcagtacagt tgcatagcca acggagctgc tgtcaaaccc
     1921 tccatccgtg gaccagtttt cccagtttcc cacagatgcc agtcctccaa ctgtaggcag
     1981 cacatatgta agtgcaataa gaggcatggc gataagcaac ccttttggta tcacctgagg
     2041 attttttact tctcctgcca gggtggaaat acactcatat ccgcagtaca tccatacaca
     2101 aatacatatc cctccgccta ttccgtcaag tatcccatag tcttccggta caaagggctc
     2161 catggggtta tagttccagt ttgcaaatcc gactacggca accactgcaa aagcgcctac
     2221 tatcagtatg gaaaagactg tactggcttt gcttacttcc cttatgccca acaggtttat
     2281 tatggtaaaa aatgctatca tggcaacttt tatggcgaag gccgttgcat cgggcatggg
     2341 tatgaactgt ctggcgtatc ccactaccag agccacataa acgccgttgg tcacataaat
     2401 agatacggtg ttccaccatc ctgcctgaaa tccccagaat tcgccgaagg ctttctgtgc
     2461 ccatacatat acaccgcctt cagccggaat tacagatccc atttcagcac agatattgct
     2521 tattggccag gcccatatta tggggaaaac gcaaagcagg gttatggtca tccccggtcc
     2581 tgcttcgggt atcatttctt ctataccgaa agctcctgct gcaacgagac agtatataag
     2641 gaacactacg ccgccaatgc gtacatcata tttcttaagt cccgcaacac cttctgtctt
     2701 ttgctgagtc atactgccgg cttggttttc cttcatgatt ttacctcctt aataaaatgc
     2761 acggtctgta aagttaagaa tgaaaaatga ggcaccaacc ctttgtgata caatg
//
LOCUS       DAONHK010000136         2814 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_29672_length_2814_cov_5.898514, whole genome shotgun sequence.
ACCESSION   DAONHK010000136 DAONHK010000000
VERSION     DAONHK010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2814
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29672_length_2814_cov_5.898514"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1303)
                     /gene="mnmG"
                     /locus_tag="PLM92_05170"
     CDS             complement(<1..1303)
                     /gene="mnmG"
                     /locus_tag="PLM92_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HPR24561.1"
                     /translation="MKSCSMGEYDVIVVGAGHAGCEAALASARLGKKTLLLAINLESV
                     AMMPCNPSIGGTGKGHLVREIDALGGEMGINIDKTFIQSKMLNTAKGSAVHSLRAQAD
                     KQLYHQEMKKTVEKQKNLDLKQEEATDIIINGGKVEGVITKTRVEYKCKAVILATGTF
                     LRGRIFIGSISYDSGPNGLSPSIMLADNLKKHGLPLRRFKTGTPARALGSSVDKSKMK
                     EQKGDDIITPFSFMNDNIEKEQISCWLTYTNEKTHDVIRENLEKSALYGGYIEGIGPR
                     YCPSIEDKVNRFSDKKRHQLFVEPEGTDTDEVYLQGMATSLPEDIQEDFYKTIEGLEK
                     LKIVRPGYAIEYDCIDPQELKMSLENRSIENLFCAGQLNGSSGYEEAAAQGIMAGINA
                     VLKIDGEEPFVLDRSEAYIGVLIDDLVTKGTGEPYRIMTSRA"
     gene            complement(1305..2714)
                     /gene="mnmE"
                     /locus_tag="PLM92_05175"
     CDS             complement(1305..2714)
                     /gene="mnmE"
                     /locus_tag="PLM92_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005999142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HPR24562.1"
                     /translation="MKDTIAAVSTAYGEGGIGIVRISGDAAEQILTDIFVPAFDAALK
                     EGHKNQRKKKVGENRKLIYGTITDPKTGEKIDEALAVFMKGPATYTKEDIVEINCHGS
                     IISLRKTLALVLSMGARPAEKGEFTKRAFINGRLDLSQAEAVIDLIKAKTDKGFGLAM
                     DQLSGKYSKEIADIRNRIKDILVQIAVNIDYPDEDIESIVYEDLKKSSSQIGDMIGFL
                     IDSANTGRIIKEGLDVVIVGRPNVGKSSLMNALLRESRAIVTEIPGTTRDTIEETISI
                     KGIPVKITDTAGIHDTDDHIERIGIEKSKSASEKADLIVFMLDASQKLTEEDREIMEA
                     IDGKKTIVLINKTDLQKKLETDEIKKTLSSCYLIETSLLSGEGLSEIEEEIEKLVYGG
                     HVKQENSLLVTNARHEMLLKEAAKALDDAGKMISKGEPLEFIEVDIKRSYDFLGEITG
                     ETVTDDIIEEVFSRFCLGK"
BASE COUNT          619 a          700 c          505 g          990 t
ORIGIN      
        1 ccgcccggga tgtcattatc ctgtagggct cacctgttcc ttttgttaca aggtcgtcga
       61 tcagaactcc tatatatgct tcacttctgt caagaacaaa gggttcttcc ccgtcgattt
      121 tcaaaacggc atttattcct gccattattc cctgggccgc cgcttcttca tatcccgaac
      181 tgccgtttag ttgtcctgca caaaacagat tttctatgct gcggttttcc aggctcatct
      241 taagctcctg gggatcgatg cagtcatatt caattgcata tccgggtctt acgatcttta
      301 atttttcaag gccttcgatg gttttataaa aatcctcctg tatgtcttcg ggaagactgg
      361 tggccatgcc ctgcaggtag acttcatcgg tatccgtgcc ttcaggctca acaaaaagct
      421 gatgtctctt tttgtcggaa aaccggttga ctttatcttc gatagaagga cagtatctgg
      481 gacctatacc ttcaatgtat ccgccgtaaa gggctgattt ttcaaggttc tcccttatta
      541 cgtcgtgggt tttctcattg gtatatgtaa gccagcatga aatttgttcc ttttctatat
      601 tatcattcat aaaagaaaag ggtgttataa tatcgtcccc cttctgctct ttcattttgc
      661 ttttatcgac ggaggagccc aatgctcttg ccggcgttcc ggttttgaat cttcgcaagg
      721 gtaaaccatg cttttttaag ttatccgcca gcattatgga tggagaaaga ccgttgggac
      781 cactgtcata ggaaatgctt ccaataaata tccttcccct taagaatgtt cctgttgcca
      841 agatgaccgc tttgcattta tattccaccc gggtttttgt tataacacct tcgacctttc
      901 ctccgtttat gattatatcc gttgcttcct cctgtttcag atcaaggttt ttctgctttt
      961 ctacggtttt cttcatttcc tgatgataaa gctgcttgtc tgcctgggct cttagggaat
     1021 gcacagcgga acccttggca gtattaagca ttttgctctg tataaaggtc ttatcgatat
     1081 tgatgcccat ctctccacca agggcatcta tttctctgac aaggtgaccc tttcccgttc
     1141 ctccgatcga agggttacag ggcatcatgg ccaccgattc cagatttatt gccaggagaa
     1201 gggttttttt gccaagacgt gcagaagcaa gagccgcctc gcatcccgca tgaccggctc
     1261 caaccacaat tacatcatat tctcccatag aacaactctt catcttactt cccaaggcag
     1321 aatctggaaa aaacctcttc tattatatcg tctgtaaccg tttctcccgt gatctcgccc
     1381 aaaaaatcat aacttctttt tatatccacc tcaataaact ccaacggttc accttttgat
     1441 atcatttttc ctgcatcatc cagagccttt gcagcttctt tcagtaacat ttcatgtctt
     1501 gcattggtca ccagtaaact gttttcctgt tttacatgtc ctccgtaaac aagtttttcg
     1561 atttcctctt ctatctccga aagcccctca ccggataata aagatgtctc tatcagatag
     1621 cagcttgaaa gggtcttctt tatttcgtcc gtctccagct ttttctgaag atcggtttta
     1681 tttatcagta caatggtttt ttttccatcg atagcttcca ttatttctct gtcttcttcc
     1741 gtgagttttt ggctggcatc cagcatgaag actataagat ccgctttctc cgatgcactc
     1801 ttgctttttt ctattcctat tctctcaatg tggtcatcgg tatcgtgaat gccggccgta
     1861 tctgttatct ttaccggaat acctttaatg cttatcgttt cttcgatggt atctctcgtt
     1921 gtcccgggaa tctcggtcac tatggctctt gactctcgaa gcaaagcatt cattaatgag
     1981 gactttccca cattgggtct tcccacgatg accacgtcaa gtccttcctt tattattctt
     2041 cctgtattcg ctgaatctat tagaaatcca atcatatcgc ctatttgcga tgagcttttt
     2101 ttcagatctt cgtaaactat tgattctatg tcttcatccg gataatcaat gttgacggct
     2161 atctgtacga gaatatcttt tattctgttt cttatatctg ctatttcttt tgaatacttc
     2221 cccgaaagct gatccatggc aagaccaaaa cccttgtcgg tcttcgcctt tataagatct
     2281 atcactgctt ccgcctggga aagatccagc ctgccgttta taaatgctct tttggtaaac
     2341 tcaccttttt cggcaggtct tgcgcccatg gacaatacca gcgccagggt tttccgaagg
     2401 gaaataatac ttccgtgaca atttatttcc actatgtctt cctttgtata tgtagctgga
     2461 cctttcatga aaacagccag ggcctcgtct attttttctc cggttttcgg gtctgttatc
     2521 gttccgtaaa tcagttttct gttttcgccg actttctttt ttctttggtt tttatgacct
     2581 tcttttaaag cggcatcaaa agccggaaca aatatatcgg taagtatttg ttccgccgca
     2641 tcaccgctta ttcttactat tcctatacct ccctcgccgt aggctgtgga aacagctgca
     2701 atggtatctt tcatttttct ccttttaaaa aatggcccgt gataccacgg gccattacgg
     2761 tttttctatt tatgctcgat tataaccctt ctgtatggtt cttcaccttc actt
//
LOCUS       DAONHK010000137         2808 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_29783_length_2808_cov_2.462405, whole genome shotgun sequence.
ACCESSION   DAONHK010000137 DAONHK010000000
VERSION     DAONHK010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2808
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29783_length_2808_cov_2.462405"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(149..1648)
                     /gene="glpK"
                     /locus_tag="PLM92_05180"
     CDS             complement(149..1648)
                     /gene="glpK"
                     /locus_tag="PLM92_05180"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011099949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HPR24563.1"
                     /translation="MEKYIMALDQGTTSSRCIIYDRKGRIKSLAQKEFRQIYPKGGWV
                     EHDAMEIWSSQLSVAQEAMAKINATHENIVSIGITNQRETAIVWDKDTGIPVYNAIVW
                     QCRRTAEYCDELKALGLTDKIREKTGLLPDAYFSATKLKWILENVNGTRKKAEEGKLL
                     FGTVETWLIWKLTGGKVHVTDYSNASRTMMFNINTMKWDNELLDIFEIPTNMLPEPVK
                     SSMVYGKTSSNIFGSEIPIAGAAGDQQAALFGQKCFRAGESKMTYGTGGFMLMNTGKK
                     PVFSKNGLITTVAWGIGDTVYYALEGSVFVAGAAIQWLRDQLGLFDDAAVSEKMALSV
                     SDSAGCYFVPAFVGLGAPYWDPYARGTILGITRGTDKNHIVRAALESMAFQTRDLADA
                     MASDLESTLTDLKADGGASANDFLMQFQADILDRDVIRPACIETTSLGAAYLAGLSTG
                     YWEDEKDIAGNCSADKRFKPSIGSKERSFRMKKWHQAVKCAMNWETAGN"
     gene            complement(1750..>2808)
                     /gene="hemW"
                     /locus_tag="PLM92_05185"
     CDS             complement(1750..>2808)
                     /gene="hemW"
                     /locus_tag="PLM92_05185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HPR24564.1"
                     /translation="KEYSENYTVDTVFIGGGTPSFASAEQTERITEAIYRDFYVSEDA
                     EITIEGNPNSLSREKLRAYKAGGINRLSMGVQSMDDEILVHLGRVHKKDHILDAYENA
                     RNAGFENINFDLMFGVPDQTLKQWENTVSEVIKLAPEHISFYSMQLEEGTSFCRNYKK
                     GLLDLTEESIVRGMYHEALRTFKNEGYMHYEISNLARPGFQCRHNLKYWSFEEYLSAG
                     LGASSYITKNLSGEMSGDPAGSITGSRFKNTESMETYLRLTGKGYLPIDLKSYHTDTI
                     KESMAVYCFTALRKTDGINFADFTKRFGMDFFRAYRERADIIFKYRKKGLLNITGTGI
                     ALTEKGIDHSNDIMSEFV"
BASE COUNT          640 a          669 c          579 g          920 t
ORIGIN      
        1 taccaccctt actgttagca ctcaaacata acgagtgcta atcatgttca taatctaaca
       61 ttattgaaca ggcatgtcaa tacttttggt aaaatttttt ataaatatgg ttttaattgt
      121 gatttcgctt gtgatctcag ttgagatttc agttccccgc tgtttcccag ttcatagcac
      181 attttacggc ctgatgccat tttttcatgc gaaaggaccg ctcttttgaa ccgatagaag
      241 gcttgaaacg tttgtcggca ctgcagttgc cggcaatatc tttttcgtcc tcccagtaac
      301 ctgtggaaag ccccgcaaga taggcggctc ccagagaagt ggtctctatg caggcaggtc
      361 ttattacatc ccgatccagt atgtccgcct ggaactgcat aagaaaatcg tttgcactgg
      421 ctcctccgtc tgccttcagg tcagtcagtg tactttcaag atcggaggcc atggcatcgg
      481 ccaggtctct ggtctggaac gccattgatt caagggcggc tcttacgata tggtttttgt
      541 ccgttcctct tgttatgccc agtatggttc ctctggcata gggatcccag tatggggcac
      601 ccagccctac gaaagcagga acaaaatagc atccagccga atcggaaacc gaaagggcca
      661 ttttttctga tactgccgca tcatcaaaaa gccccagttg gtctcttaac cactgtatgg
      721 ctgcacctgc cacaaagaca gagccttcca gggcataata aacggtgtct cctatccccc
      781 aggctacagt cgttataagt ccgtttttgg aaaataccgg tttcttgcct gtgttcataa
      841 gcatgaaacc gcctgtgccg tatgtcattt tactttctcc ggctctgaag catttctgtc
      901 cgaaaagcgc tgcctgctga tcccctgctg cacccgcaat gggtatctcg cttccgaaga
      961 tgttagaaga tgtttttcca tagaccatgc tgctttttac cggctcgggc agcatattag
     1021 tgggtatttc aaatatatca agaagttcat tgtcccattt catggtattt atattgaaca
     1081 tcatggtcct tgatgcattg gaataatcag tcacatggac ttttcctccg gtaagtttcc
     1141 atatgagcca ggtttccact gtgccgaaaa gaagtttgcc ttcttcagcc ttttttcttg
     1201 ttccgtttac attttcaagt atccatttta gtttggttgc tgaaaaataa gcatcgggta
     1261 aaagaccggt tttttctctt attttatctg taagtcccag ggctttcagt tcatcgcagt
     1321 actccgcagt ccttcggcac tgccatacaa tggcattgta aacaggtatt ccggtgtctt
     1381 tatcccatac gatggcggtc tctctttgat tggttattcc tatggaaacg atgttttcgt
     1441 gggtggcgtt tatctttgcc attgcctcct gagccaccga aagctgagaa gaccatattt
     1501 ccatggcatc atgttcaacc catccgccct ttggatatat ctggcggaat tctttctggg
     1561 caagactttt gatccggcct tttctgtcat aaataatgca cctggagctg gtcgttccct
     1621 ggtcaagggc cataatatat ttttccatgg ttgttctcct tttcccggtt ttgcaccgga
     1681 tacacttcaa atgtatgtct cgtgtatatc taatgtatat ctaatgtata tctcatgctg
     1741 aggtcgatcc tatacgaatt cgctcattat atcatttgaa tgatctatcc ccttttctgt
     1801 aagggcgata cctgttccgg tgatatttag aagtcctttt tttctgtatt tgaatattat
     1861 gtctgctctt tctctatatg ccctgaaaaa atccatcccg aatctctttg tgaaatcagc
     1921 gaaatttatg ccatccgtct ttctgagagc agtgaaacag tatacggcca tgctttcttt
     1981 gatggtatcg gtatgataac ttttcaggtc gatgggcaga tatccttttc ctgtaagcct
     2041 caggtatgtt tccatacttt cggtattttt gaacctgctg cccgtaatcg atcctgccgg
     2101 atccccgctc atttctccgg aaaggttttt tgttatatat gaagaggcac cgagtccggc
     2161 agaaaggtat tcttcaaagg accagtattt cagattatgc cggcattgaa acccgggcct
     2221 tgccaggttt gatatctcat aatgcatata accttcgttt ttgaatgtcc ggagggcttc
     2281 atggtacatc cctctgacta tgctttcttc tgtaagatcc agcagtccct ttttgtaatt
     2341 tctgcagaag gaagttcctt cctcaagttg catgctgtaa aaagaaatgt gctcgggagc
     2401 cagttttata acttcgctga cggtgttttc ccactgcttc aaagtctggt ccggcactcc
     2461 gaacatgaga tcaaaattga tgttttcaaa acctgcattt cgtgcgtttt catatgcatc
     2521 caaaatatgg tcttttttgt ggaccctccc gaggtggacc agtatctcat cgtccatgga
     2581 ctggactccc atgctgagcc tgtttatgcc tccggcttta taggctctga gtttttccct
     2641 tgaaagagag tttgggttcc cttctattgt gatctccgca tcttctgaaa catagaaatc
     2701 cctgtatatt gcttcagtta ttctctccgt ttgctctgct gaagcaaaag acggagttcc
     2761 gcccccgatg aaaacagtat ccacagtgta attttcactg tactcttt
//
LOCUS       DAONHK010000138         2720 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_31302_length_2720_cov_3.257036, whole genome shotgun sequence.
ACCESSION   DAONHK010000138 DAONHK010000000
VERSION     DAONHK010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2720
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31302_length_2720_cov_3.257036"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="PLM92_05190"
     CDS             complement(<1..318)
                     /locus_tag="PLM92_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HPR24565.1"
                     /translation="MDHPKEEIKKILGLLRNWRRDLHMIPEPGSEVTKTRSYIHSVLK
                     KMNCRIIPAAQNGICAFFDFDRPDTAAYRSDMDALPMTESGDLSYASKHPGFMHACGH
                     DGHM"
     regulatory      488..651
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            775..2052
                     /gene="lysA"
                     /locus_tag="PLM92_05195"
     CDS             775..2052
                     /gene="lysA"
                     /locus_tag="PLM92_05195"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008691072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HPR24566.1"
                     /translation="MGIMEIGKVSVTRLAEKCGTPLLIYDEEKIENKIKSYMRNFSSP
                     LFQTEVVYASKAFGCPAMIEKVKDGGASLDVVSGGELYIAKVSGMNMEKVYFHGNNKT
                     SEEISTALEFGCGNIVADNLEEFRTIIETAACTKRKINLMLRINPKVAAHTHEYTVTA
                     AEDSKFGVSIDHKEKIAVLIKTAADKKVTFKGFHSHIGSQIFEASAFIKAAGLMIGLC
                     SYMEKEHDIPCPWLSLGGGFGIKYTAEDDPPETETLCRELIRGCEEAIRSAGVKTEKI
                     MIEPGRSIAGDAGCTVYKVGYEKIAGDKRYLFVDGGMGDNIRPALYGAEYPCDLAEHM
                     GDTKDKEYVIGGKYCESGDIIIRKAMLPEAKKGDLLIVYSTGAYAYSMSSCYNGTGRP
                     AVVFVKDGRTRLVMRREKKEDLMYLAKDQEVKI"
     gene            2049..>2720
                     /locus_tag="PLM92_05200"
     CDS             2049..>2720
                     /locus_tag="PLM92_05200"
                     /inference="COORDINATES: protein motif:HMM:NF012899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPR24567.1"
                     /translation="MNTVQQYGSECIDCTDTIKIIAKETAKKKNPQDGLVIVVSAMGS
                     IAESIMTNAGNIAENMVREDPHALFSAGERQTAALMAVALEEAGLNAQVVTDIRGRSL
                     EAKDHEESTVEIDTGKIEKLLDEGTIVVVTGSQGIGDHELKDMDRRCGASSTAVVIAA
                     QLGCECELYGNAEGIHSVDPAVCPGSKILKTSCYEEAMEMVLLGEANLESRALELAGM
                     YNVKLH"
BASE COUNT          851 a          515 c          699 g          655 t
ORIGIN      
        1 catatgtccg tcgtgtccgc aggcatgcat aaaaccggga tgttttgatg cataagaaag
       61 atcaccgctc tctgtcatag gaagagcatc catgtcagat ctatatgctg cagtatccgg
      121 tctatcgaaa tcaaagaaag cacaaatccc gttttgtgct gctggtatta tcctgcagtt
      181 cattttcttc agtactgaat gaatataact gcgggtcttt gtgacctcag atcccggttc
      241 ggggatcata tgcaggtctc tgcgccaatt tctcaaaagc cctagtattt tttttatttc
      301 ttccttggga tgatccataa tatctcctct gcgggatgat ccataatatt ccctctgtat
      361 atcctctttt cggcatatat tatctcaaaa aacagcggct gtcaaaaact tttgttaaaa
      421 acaacaaaaa atccttgcat actgcaaact attattgtag aatgaacaac ataaatcaaa
      481 attcaataat catgatagag gcgcgaagca gaagaagata tgggagccgg caggcagaga
      541 tcatatcgga ggcaatcttc gccgaatcgg tcaggcaggc atgccgggtc ggtgggcata
      601 tggtcaatag ccatatgact gtctacgttc agtagttgcg ctatctttta atgtatgcat
      661 agttttatgt gtatgtttct tgggaccggc agcgcatgcc ggttttctat ttctccggac
      721 aggatatgcg taccattggg caaaaaactg aaagtgaaag gacattaaag taaaatgggg
      781 atcatggaaa taggcaaagt gtctgttacc cgtctggcag aaaaatgcgg aacaccgctt
      841 ttgatctacg acgaagaaaa gatcgaaaac aaaataaaaa gttatatgag gaatttttct
      901 tccccgcttt ttcagactga ggtggtttat gcatccaagg cttttggctg tccggcaatg
      961 atagaaaaag tcaaagatgg cggcgcctcg ctggatgttg tcagcggagg agaactttat
     1021 attgccaagg tgtcaggcat gaacatggaa aaagtatatt tccatgggaa caataaaact
     1081 tctgaagaaa tcagtacggc attggagttt ggctgtggaa atattgtggc ggataatctt
     1141 gaagagttcc gtacaattat tgaaaccgca gcctgcacga aacgaaagat aaacttgatg
     1201 ttgcggataa atccgaaagt cgctgcccat actcatgagt atacagtcac ggcagcagaa
     1261 gattcaaaat tcggagtttc catagaccat aaagaaaaaa tcgcagtcct gataaaaact
     1321 gcagcagaca aaaaggtgac cttcaaaggt tttcattcac atataggttc tcagatattt
     1381 gaagcatctg cctttataaa ggctgccgga cttatgatcg gtctttgcag ctatatggaa
     1441 aaggagcatg atatcccttg tccgtggctg agcctgggag gaggattcgg aataaaatat
     1501 accgctgaag atgatccgcc ggagacagag accctttgca gagaactgat caggggatgt
     1561 gaggaagcga taagatccgc cggtgtaaaa accgaaaaaa tcatgatcga gccgggcagg
     1621 tccatagcgg gagatgcggg atgtacagtt tataaagtgg gatatgaaaa gattgcagga
     1681 gataaaagat atctgtttgt agacggggga atgggagata atatacgccc tgccctttac
     1741 ggggcagaat acccctgtga cctggcagag catatgggag atacgaagga taaagagtat
     1801 gtgataggcg gtaaatattg tgaatccgga gatataatca tcagaaaagc aatgctgccc
     1861 gaagccaaaa aaggcgatct gcttatagta tattccacgg gtgcctatgc atattccatg
     1921 agcagctgtt acaacgggac aggacgtccg gcagtagtat ttgttaaaga cggcaggacg
     1981 cggctggtaa tgcgacggga gaagaaagaa gatcttatgt atttggcaaa agatcaggag
     2041 gtgaaaatat gaatacagta cagcagtacg ggagtgagtg tatagactgt acagacacta
     2101 taaaaatcat agcgaaagaa acagccaaaa agaaaaaccc gcaggacggt cttgtgatag
     2161 tagtctctgc catgggaagt atagcagaaa gcataatgac aaatgcagga aatatagcag
     2221 agaatatggt ccgggaagat ccgcatgctc tgttttccgc cggagaacga cagacagcag
     2281 cacttatggc tgtagccctt gaagaagccg ggctgaatgc acaggtcgta accgacataa
     2341 gaggaaggag ccttgaggca aaagatcatg aagaaagcac cgtcgaaata gacaccggca
     2401 aaatagaaaa actgctggat gaaggaacca tagtcgtagt tacaggttct cagggaatag
     2461 gggatcatga gctcaaggac atggaccgca gatgcggggc atcttccaca gcggtggtca
     2521 ttgcggcgca actgggatgt gaatgtgagc tttacgggaa tgctgagggg atccattctg
     2581 ttgatcccgc agtgtgtccc ggttcaaaaa tcctgaaaac ttcgtgctat gaagaagcca
     2641 tggaaatggt attgctgggc gaagcgaatc tggaaagcag ggcattggag ctggccggta
     2701 tgtataacgt aaaacttcat
//
LOCUS       DAONHK010000139         2708 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_31537_length_2708_cov_3.680362, whole genome shotgun sequence.
ACCESSION   DAONHK010000139 DAONHK010000000
VERSION     DAONHK010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2708
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31537_length_2708_cov_3.680362"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..997
                     /locus_tag="PLM92_05205"
     CDS             <1..997
                     /locus_tag="PLM92_05205"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IclR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HPR24568.1"
                     /translation="PFQLMQKVSEEYGCNGSFKVADGASYAKTMKDCVSWGPVFPGDF
                     MAAHVEDERLSVDTMMLATKLYTSFLARTASDIDKKQKLEDMTSLDKALYILSFFTEE
                     PYRYSVGHIVKLTGINRTTVYRNLVSLQKAGLLIREEENGRYSLGPETYRMGNVYLTN
                     ADYKDNILGILEDIGAETRESVGLARLEGDKIVSIYSVESHQSIKMNDRPGTFYPVNK
                     GTYGKCLMAFSEEPVTADMLKKQNFKKTGPNTLTEPEEILAEYEKIRAQGYVLSIEET
                     MNYVIGVGVPLKSHDGHVKNVVAASFFKQDDYLEKIEKIKDILFKYKKELEKFIN"
     gene            1227..2153
                     /locus_tag="PLM92_05210"
     CDS             1227..2153
                     /locus_tag="PLM92_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015925114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC/Alx family metal homeostasis membrane
                     protein"
                     /protein_id="HPR24569.1"
                     /translation="MDTDSKNIKNIGEEELRNDHRLRRTVKAYKWLFFWIGLAFLFNL
                     GVYFFFGTEKAIEFLGGYLIEFSLSIDNVFVFLMIFMSFNISEHAQHRVLSYGIAGAI
                     VLRFIFIFFGVSVVNKFEWVLYIFGVILIISAVRMLKKEDKEKDPHDSPIIRVMKKII
                     PMTTYFQDEKFFVKEKAKLMATPLFAVLVLIESSDIIFAIDSVPAVISVSRDLFIVYT
                     SNIFAILGLRQLFFVVEHMQERFQYVRYGVAVLLAFTGVKMLLGMAHIHISTPISIGI
                     IAGVLVTSIIVSVIVSKYKEKKAAKKEETDNV"
     gene            2316..>2708
                     /locus_tag="PLM92_05215"
     CDS             2316..>2708
                     /locus_tag="PLM92_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006000393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HPR24570.1"
                     /translation="MFKKLSEKPIAEVQNAQAEKWEKEDLLKKCLSSRENSPSFIFYE
                     GPPTANGKPGIHHVMARTLKDSVCRYKTMRGYKVNRKAGWDTHGLPVEIEVERQLGMT
                     GKQDIEKYGIAKFNEKCKESVFIYYNQWE"
BASE COUNT          904 a          482 c          622 g          700 t
ORIGIN      
        1 accctttcag cttatgcaga aagtttcaga agaatacggt tgcaacggca gcttcaaagt
       61 ggctgacgga gcatcctatg ccaagaccat gaaagactgt gtatcctggg gaccggtatt
      121 tccgggggat tttatggccg cacatgtaga ggatgaaaga ttatcagttg atactatgat
      181 gcttgcaaca aaactctata cttcatttct tgcaaggact gccagtgata tagacaaaaa
      241 gcaaaagctt gaagacatga caagccttga caaagccctt tatattttgt cattctttac
      301 ggaggaacct tacagatatt cagtaggtca tattgtcaag ctgacaggca taaacaggac
      361 tactgtttac agaaatctgg tgtcactcca aaaagcgggg cttctcataa gagaagagga
      421 aaatggccga tattccctgg gacccgaaac atacaggatg ggtaacgtat atcttaccaa
      481 tgcggactat aaagataata tattgggcat actggaagac ataggggcag aaacaaggga
      541 gtctgtgggg ctggcccgcc ttgaaggaga caaaatagtt tcgatatatt ccgtggagag
      601 tcaccagtcc ataaaaatga acgacagacc cggcaccttt tatcccgtga acaagggtac
      661 atatggtaaa tgtcttatgg ccttcagcga agagcctgtt acggctgata tgctcaaaaa
      721 gcagaatttc aagaaaacag ggccgaatac tctgacagaa cctgaagaga tcctggccga
      781 gtacgaaaaa ataagggccc agggttatgt gctgagcata gaggaaacaa tgaactatgt
      841 tataggggtg ggagtacctt taaaaagcca tgacggccat gtaaagaatg tggtggcagc
      901 atcatttttc aagcaggatg attatctgga aaagatagaa aaaatcaagg atatcttatt
      961 caaatacaag aaagagcttg aaaaattcat aaattgaaaa acaaattgac gaattttttc
     1021 cactatggta ctattagtgc gaagatatgt taaggggagt agcttgaaag cgacaggacc
     1081 aacaatcgcg gcttatgcct ggttctttcc tccggagtgg aagcgagacc ttaatgtttg
     1141 ctttttggca gacattaagg cttttatatt ttatggtgct atccggaatc aaagaccgga
     1201 agaccaaaaa cataaaaagg agaaaaatgg ataccgacag caagaacata aaaaatatag
     1261 gggaagaaga actccgtaat gaccacaggc tcagaagaac tgttaaggct tacaaatggc
     1321 tttttttctg gatcgggctt gcttttcttt tcaatctagg agtgtatttt ttctttggaa
     1381 cagaaaaagc catagaattt cttggcggat atttgattga attttccctc agtatagata
     1441 atgttttcgt atttctgatg atattcatga gtttcaatat aagtgagcat gcacagcaca
     1501 gagtactgag ctacggtatt gcaggagcca tagtcttaag atttatattc atatttttcg
     1561 gtgtttcagt tgtaaataaa tttgagtggg tgctttatat ttttggtgtc atacttatta
     1621 taagtgcagt aaggatgctg aaaaaagagg acaaagaaaa ggatcctcat gacagcccca
     1681 taatccgtgt gatgaaaaaa ataataccga tgaccacata ttttcaggat gagaagttct
     1741 ttgtaaaaga aaaagcaaaa ctcatggcaa caccgctttt tgcggtgctg gtgctcatag
     1801 agagttctga tataatattc gccatagact cggttccggc ggtcatatcg gtttcaagag
     1861 acctttttat agtatataca tcaaatatat ttgccatact gggactcagg cagcttttct
     1921 tcgtagttga acatatgcag gagcggtttc agtatgtgcg atacggtgtg gcggtacttc
     1981 tggcatttac aggggtgaaa atgcttttgg gaatggccca catacatata tccactccga
     2041 tttccatcgg aataatagcg ggggtccttg tcaccagcat aatagtgtct gttatcgttt
     2101 caaaatataa agaaaaaaaa gccgcaaaaa aagaagagac agacaatgta taagactcac
     2161 ttttacgaga ctctaaataa tgcctgcaga gtttgatttt tgttgacatt gatgccttaa
     2221 gacaatataa tagaggcgtg cgaatgcgca gaaaatcgtc ccctgatcgg cagacgccgg
     2281 gcagggattt tatttatcaa ggagacaaca aagaaatgtt caaaaaatta tcagaaaaac
     2341 ctatagcaga agtgcaaaac gcacaggccg aaaaatggga gaaggaagac ctcctgaaaa
     2401 aatgcctttc ttcaagagaa aacagccctt cattcatttt ttacgagggg cctcccaccg
     2461 ccaacggaaa accgggtata caccatgtga tggcaaggac cctgaaagac tcggtttgtc
     2521 gttacaaaac gatgcgggga tataaagtaa acagaaaagc cggatgggat actcatggac
     2581 ttccggttga gatagaagta gaaagacagc tgggaatgac cggcaaacag gatatagaaa
     2641 aatacggcat tgcaaagttt aacgaaaaat gcaaagaatc tgttttcata tattacaatc
     2701 agtgggaa
//
LOCUS       DAONHK010000140         2681 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_31996_length_2681_cov_3.066260, whole genome shotgun sequence.
ACCESSION   DAONHK010000140 DAONHK010000000
VERSION     DAONHK010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2681)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31996_length_2681_cov_3.066260"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..669
                     /locus_tag="PLM92_05220"
     CDS             <1..669
                     /locus_tag="PLM92_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018593795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPR24571.1"
                     /translation="HISFYVIGFLALTGTLLGRFVCGWLCPFGLVQDLLFKIPFIKKI
                     RKVPGDRILKGLKYFILAIFVILLPIFVSGSFGQGDPYFCKYICPSGTLMAGWPLMLL
                     NSGLRATAGFLFTWKSIILVAVILISIIIYRPFCRWACPLGAIYGMCNRISLYRYEVD
                     SEKCTQCGKCQSVCKLDIKMYENPNSAECIRCGDCQRACPEGAITVPIMKNKLPIKKE
                     VENE"
     gene            662..1003
                     /locus_tag="PLM92_05225"
     CDS             662..1003
                     /locus_tag="PLM92_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011591025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HPR24572.1"
                     /translation="MNDCIFCKIAAKEIPSKAVYEDEEIYCFHDLEPQAPVHVLIIPK
                     KHIASTDEIMPGDLELMGHMMGTIGKIASGLGLENGYRVVMNCGEDGMQTVNHLHMHL
                     LGKRKLTWPPG"
     gene            1061..1243
                     /gene="rpsU"
                     /locus_tag="PLM92_05230"
     CDS             1061..1243
                     /gene="rpsU"
                     /locus_tag="PLM92_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009644705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HPR24573.1"
                     /translation="MAQVIVKENESLESALKRFKRSCARDGVMSELRKREHYEKPSVK
                     RKKKSEAARKKANKKY"
     gene            1290..1715
                     /locus_tag="PLM92_05235"
     CDS             1290..1715
                     /locus_tag="PLM92_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009345559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HPR24574.1"
                     /translation="MADFKEAMKSHDEVGKNTITFVRAAIKQAEVDTRTELSDEEIIL
                     IIAKQIKMRKDAIKDFEKGGREDLAKAYKAEIEILSGYMPPQISEEEIRKIVRDTAAE
                     LGIEGGSENMGKLMGRVMQNVKGTADGGDVRKIVQEFLA"
     gene            1777..>2681
                     /locus_tag="PLM92_05240"
     CDS             1777..>2681
                     /locus_tag="PLM92_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013275724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HPR24575.1"
                     /translation="MNNQVKVEIDDTLDRSELFGIMDKNLKIIKDNFSVEIIQRGNQI
                     TLKGDDAEKAGTVIREMLDIISSGEKIDSQKINYIMTLAEKGVSYQESSIGKDILCYT
                     HMGKPIKPKTLGQKSYVASIKNKDVVFCIGPAGTGKTYLAVAMAVSAFKHKDVQKIIL
                     ARPAVEAGESLGFLPGDLQEKVDPYLRPLYDALYDILGRDSALRLKEKELIEIVPLAY
                     MRGRTLDNSFIILDEAQNTTKEQMKMFLTRMGFGSKMVITGDVTQIDLPKGKHSGLVE
                     VTKILKEVKGIDFSYLKDADVVRHD"
BASE COUNT          902 a          453 c          650 g          676 t
ORIGIN      
        1 catatatcgt tttatgttat tggttttctt gctttgacag gtaccctttt gggcaggttc
       61 gtatgcggat ggctttgtcc ttttggtctt gtgcaggatc tccttttcaa gataccattt
      121 ataaaaaaga taaggaaagt gccgggggac aggatactaa agggactgaa atattttata
      181 cttgccattt ttgtgattct cctccccatt tttgtatcag gttcatttgg tcagggagac
      241 ccttatttct gtaaatacat atgtccttcg gggactctta tggcaggatg gcctcttatg
      301 cttttgaaca gcggattaag ggcaaccgca ggattccttt ttacatggaa gtcaattatt
      361 ctggttgcgg tcatacttat ttcaataatc atatatcgcc cattttgcag atgggcatgt
      421 cctctggggg cgatttacgg catgtgcaac aggatatctc tttaccgcta tgaggtagac
      481 agtgagaagt gtacacagtg cggtaaatgt cagagcgttt gcaagttgga tataaagatg
      541 tatgagaacc caaacagtgc agagtgtata aggtgcggcg actgtcaaag ggcctgtccg
      601 gaaggagcca taacagtgcc gataatgaaa aacaagttac ctataaaaaa ggaggtcgaa
      661 aatgaatgat tgtatatttt gtaaaatagc cgcaaaagag atcccgtcaa aagctgtcta
      721 tgaagacgaa gagatctatt gtttccatga tctggaacct caggcccctg tccatgttct
      781 tataataccc aaaaaacata tagcatcaac agatgagatc atgccgggcg atcttgagct
      841 tatgggccat atgatgggta cgataggcaa gatagcttcc ggtcttggtc ttgaaaacgg
      901 atacagagta gtaatgaact gtggagaaga cgggatgcag acagtaaatc atctgcatat
      961 gcaccttttg ggaaagagaa agttgacatg gcctcccggt tgatagtata atattatggt
     1021 tgcaggcaag atattgtaac gtcaggaggt gagtaagaat atggcacagg taatagtaaa
     1081 agaaaacgag agccttgaaa gcgctctaaa aagattcaag cgttcctgcg cacgcgatgg
     1141 cgttatgagt gaacttagaa aaagagagca ctacgaaaaa cccagcgtaa aaaggaagaa
     1201 aaaatcagaa gctgcaagga aaaaggcaaa caaaaagtat taagcttttt gaagagaggt
     1261 gttttttatt ggctttaaaa gaaaaattga tggctgactt caaggaggcc atgaagtctc
     1321 acgatgaagt cggcaaaaat accataacat ttgtaagggc tgccataaaa caggcagaag
     1381 tcgatacccg tacagaactt tccgatgaag aaataatact cataatagca aaacagatca
     1441 aaatgagaaa agatgctata aaggactttg aaaaaggcgg cagagaagac ctggcaaagg
     1501 catacaaagc tgagatcgaa attttatccg gatatatgcc gccgcagata tcggaagagg
     1561 aaataagaaa aatcgtaagg gatacggctg ctgaactggg tatagaaggc ggcagcgaaa
     1621 atatggggaa actcatgggt cgtgtcatgc agaatgtcaa aggcactgct gatggcggtg
     1681 atgtaagaaa aatagtccag gagttccttg catagaataa aacatatttg cggcaacccg
     1741 atttatcggg ttgctcttta taaaggagaa tattttttga ataaccaagt taaagtcgaa
     1801 atagatgata cactggaccg tagtgagctt ttcggcatca tggacaaaaa tctaaaaatc
     1861 ataaaagaca atttttctgt tgaaataatc cagagaggga atcaaataac ccttaaggga
     1921 gatgatgccg aaaaagccgg aactgttatc agagagatgc ttgacataat atcatcggga
     1981 gagaaaatag attctcagaa aatcaactat ataatgacac tggcggaaaa gggagtatca
     2041 tatcaggaaa gcagcatagg taaagatatc ctttgttaca cccacatggg aaagccgata
     2101 aaaccaaaga ctctgggaca gaaatcatat gtggcttcaa taaagaacaa agatgtggtt
     2161 ttctgtatcg gccctgcagg tacaggtaag acatatctgg cagtggccat ggctgtgagc
     2221 gcgttcaagc ataaagatgt acagaagata atactggcaa gacctgctgt ggaggcggga
     2281 gaaagtcttg gattccttcc cggagatctg caggaaaaag tcgatcccta cttaagacct
     2341 ctttatgatg ccctttatga tatattggga agagacagcg cattgagact aaaggagaaa
     2401 gaattgatag agatagttcc tctggcatat atgagaggcc ggactctgga taattctttt
     2461 ataatactgg atgaagccca aaatactacc aaagaacaaa tgaagatgtt tttgacaaga
     2521 atggggttcg gatccaaaat ggtcataacg ggagatgtga cccagataga ccttcccaag
     2581 ggcaaacatt cgggacttgt ggaagtcaca aaaatactca aagaagtcaa aggaatagat
     2641 ttttcttatc ttaaagatgc ggatgttgta agacatgatt t
//
LOCUS       DAONHK010000141         2674 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_32115_length_2674_cov_3.524246, whole genome shotgun sequence.
ACCESSION   DAONHK010000141 DAONHK010000000
VERSION     DAONHK010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2674
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32115_length_2674_cov_3.524246"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      68..187
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            259..1929
                     /locus_tag="PLM92_05245"
     CDS             259..1929
                     /locus_tag="PLM92_05245"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HPR24576.1"
                     /translation="MVKKAKTRIITTITALVMIFSVGMFFPDFASADSLFDQKIARNG
                     LDYEEVTGDNATVFTIFVSDAAKDFSNPQNRGDGFQGRWKMLTQSQLDSLDTTDGIVL
                     IFGNQSTHQWLYGPVYYSRCTAEGAYEQDIVAKGLKLEAIAYAMGYKPDNDSKTRITA
                     IGADGTKTDAGSKAFIERYYYTQDNNDPGVPVFEILKLNGTGSLPSFRMGQETPTDKN
                     KWKDNIKGIQIDAVTTATQKRDVVYTVRSGSTISEYVMNDIVDAGEYKAKYYYYNENS
                     NEVTADVKGAPLKNVLSQKGIVLGKNDAISAENKRGTLKELSTGDASKYFLAYSGTEK
                     TGETETALESDSEFCLFGPGNTVSEVKFRDIYGIKVTRAPQAPQIAASSKGYRSIKIS
                     WNIKTNAKGYEIFNITANKKIYVSGGTKTYYVQEGLKTGKKYTYKVRAYNLKENGSKV
                     FGDYSSSKAAVPVPAKPSSIKLYKKSRAVKVKWSAVKGASGYQVYMATKKNGKYKNVK
                     NIKSAKTKVYTKTKLKKGKKYYFKVRAYRTVGKSKIKGQWSAVKYRKL"
     gene            1948..>2674
                     /locus_tag="PLM92_05250"
     CDS             1948..>2674
                     /locus_tag="PLM92_05250"
                     /inference="COORDINATES: protein motif:HMM:NF014427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HPR24577.1"
                     /translation="MRRKATKKRMIAFIVSLSMVASLIPVMAFADVTEVSLNKTKLTM
                     ETESTYDLTADVTVTEGEATTVTWSSDDTDVATVTDGVVTAVSEGTADITATSTADDT
                     KMATCRVTVYENALTIVLGTRADPIVVKEFRNADINALTSSGPFMYGGFNHYFTWSAT
                     KNKSTGVDEPKSGPSIDTIFEAAGIDVDAIDDDTVISFIPSDDTTGQYTANFTKAQLF
                     ADRYYYRYSSELTQGGFLTSGKEV"
BASE COUNT          945 a          484 c          614 g          631 t
ORIGIN      
        1 gtattgaatt tttatatata attatacaaa aatctgttga ttaaagcacc tttaactgat
       61 aaactaataa caatcgtgaa tccgagtttc gattctaaag gccaaaggcc catgaagaga
      121 gaccgatagg gcaggcggga aactgctgag ccttccgtat ttgaaaagga gccgcgatca
      181 ctgaagccgc agatttaagg ctgcagactc cttttttgta aggagttatt tttttggcat
      241 aaagcaggca taggaataat ggtaaagaaa gcaaagacaa gaattattac gactatcacg
      301 gcattagtga tgattttttc agtggggatg tttttcccgg attttgcaag tgcagacagc
      361 ctgtttgacc agaaaatcgc aagaaacgga ctcgactatg aagaggtaac aggggataat
      421 gctacggtat ttaccatatt tgtttcagat gcagccaaag atttcagtaa tccacaaaat
      481 agaggagacg gatttcaggg tagatggaag atgctgactc aaagtcagct tgattctctg
      541 gatacaacag atgggatcgt gctcatattc ggaaaccaat ccacccatca atggctatac
      601 ggcccggtat attattcgcg ttgtacagcc gaaggagcct atgagcagga tattgtggct
      661 aaagggttaa aacttgaagc aatagcctat gcaatgggct ataaaccgga taacgattcc
      721 aaaacaagaa taacggctat aggagcggac gggacaaaaa cagatgccgg cagcaaggct
      781 ttcatagaac ggtattatta tactcaagat aataatgatc cgggagtgcc ggtttttgaa
      841 atactgaaac tgaatggtac aggcagtttg ccatcattca ggatggggca ggaaacccct
      901 acagataaaa acaaatggaa agataatata aaaggaatac agatcgatgc ggtaacgact
      961 gccacccaaa aacgagatgt ggtttataca gtcagatccg gcagtacaat ttccgaatat
     1021 gtcatgaatg acatagttga tgcgggagaa tataaggcga aatattatta ttataacgaa
     1081 aactcaaatg aagtgactgc tgatgttaaa ggtgcacctt tgaaaaatgt tctttcacaa
     1141 aaaggaatag ttcttggcaa gaatgatgct atttcagccg aaaataaaag aggtacttta
     1201 aaagagcttt ctacggggga tgcatcgaag tattttcttg catacagcgg cacagagaaa
     1261 acaggagaga cagaaactgc actggagagc gatagcgagt tttgtctttt cggtcccgga
     1321 aacacggtgt cggaagtcaa attcagagat atttacggga tcaaggtgac cagggcacct
     1381 caggctcccc agatcgcagc ctcatcgaag ggatacagga gtataaagat atcctggaac
     1441 ataaagacaa atgctaaagg atatgaaatt tttaatatta cagccaacaa aaaaatatat
     1501 gtttcggggg gaactaaaac atactatgtt caagaagggc taaaaacagg caaaaaatac
     1561 acatataaag tcagagcata caaccttaag gaaaacggaa gcaaagtatt tggtgattat
     1621 tcttcttcca aggcagcagt tcccgtacct gccaagccgt caagcatcaa actttacaaa
     1681 aaaagcagag ctgtaaaggt aaaatggtcg gcagtcaaag gcgcatcggg atatcaggtg
     1741 tacatggcca caaagaaaaa cggaaaatac aaaaatgtga agaatataaa gtcggctaaa
     1801 accaaagttt acacaaaaac aaaactcaaa aaagggaaaa aatattattt caaagtcaga
     1861 gcctatagaa ccgtaggcaa aagcaagatc aaggggcaat ggtctgcggt gaaatataga
     1921 aaattatagt attacaagga ggaaagaatg agaagaaaag caactaaaaa gcgcatgatc
     1981 gcttttattg tttccctgtc tatggtggca tcccttatcc ctgtcatggc atttgcagat
     2041 gtaacggaag tctctcttaa caagacgaaa ctcaccatgg aaactgaaag tacatacgat
     2101 ttaacggctg atgttacagt gactgaagga gaagcaacta ccgtaacctg gtcaagtgat
     2161 gatacggatg tggctacggt tacggacggt gttgtaactg cagtatcaga aggtacagcg
     2221 gatatcacag caacttccac tgcagacgac acaaaaatgg ctacatgccg ggtgactgtg
     2281 tatgaaaatg cacttacaat agtgttggga acaagggcag atcccatagt agtaaaggaa
     2341 ttcagaaatg cagatatcaa tgcattgact tcaagcggac catttatgta tggaggcttc
     2401 aatcactatt ttacctggtc tgccacaaaa aacaaatcaa caggtgtaga tgaacccaaa
     2461 tcgggtccca gcatagatac tatttttgaa gcggccggaa tagatgtgga tgccattgat
     2521 gatgatacag ttatttcatt tataccctcc gatgatacca ccggacagta tacagccaac
     2581 ttcacgaagg cccagctttt tgcagacaga tactattaca gatattcaag cgaactgaca
     2641 cagggaggat ttttgacaag cggcaaggaa gttg
//
LOCUS       DAONHK010000142         2672 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_32148_length_2672_cov_4.833779, whole genome shotgun sequence.
ACCESSION   DAONHK010000142 DAONHK010000000
VERSION     DAONHK010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32148_length_2672_cov_4.833779"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            264..1556
                     /locus_tag="PLM92_05255"
     CDS             264..1556
                     /locus_tag="PLM92_05255"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HPR24578.1"
                     /translation="MKRLQEFMKNKMMKSYSFFIIFTVAILCIIYFIIKNFDLIAVTT
                     GSAILSILDALTPLFIGLLLAYIINPLVNGIDNRFMNRIFKDPPPLSGNENPTAKRKH
                     KRKKYRSRLASIILTYVIIIILISLLIYLLASLLVGELLLESLPAAAGDIADNLALYE
                     KNLRTWVSDLPGGVVSEKINDAADAILSWISKNLSAGAIVSAVTSVFGSIVNFVIGLI
                     ISIYLLIDKDFFKHLWNGFLDLVLPQKACSAINGTLTEINDVLSRFIRGVLIDAVVVA
                     TLSSIGLTIAGLEFAVFIGIFAGICNIIPYFGPILGMIPAFLVGFITEGLWQGVIAVI
                     ILLVIQQIDGNLIYPKVVGSSTGLHPLFVLLAVSIGSYYAGIAGMVLAVPVAGVLQIF
                     CYRWIAFQQKRKEDRNQEGGNDREREQETDIKQTVKKP"
     gene            complement(1646..1987)
                     /gene="rplQ"
                     /locus_tag="PLM92_05260"
     CDS             complement(1646..1987)
                     /gene="rplQ"
                     /locus_tag="PLM92_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HPR24579.1"
                     /translation="MPGYKKLGRDSAHRKSMLRNLVTDLLREERISTTNDRAKEARRQ
                     AEKMITLAKRGDLHARRQVMAYVYDEDVVKKLFDDIAPKYADRNGGYTRILKLGPRQG
                     DSAEVVFLELV"
     gene            complement(2006..>2672)
                     /gene="rpoA"
                     /locus_tag="PLM92_05265"
     CDS             complement(2006..>2672)
                     /gene="rpoA"
                     /locus_tag="PLM92_05265"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HPR24580.1"
                     /translation="KRVIINAVGPKEVTAADIIADADMEIFNPGLHIATLEENASLVM
                     EINLANGRGYVPAEQNKTESTPIAVIPVDSIFTPVVKVNFTVENTRVGQVTDYDKLIL
                     EIWTDGSISPEEGVSIGAKIMQEHLNLFVQLTDDTESMEIMVEKEEDQKEKALEMTIE
                     ELELSVRSFNCLKRAAINTVEELTHKSEDDMMKVRNLGKKSLDEVKLKLEELGLGLRE
                     TDE"
BASE COUNT          711 a          584 c          524 g          853 t
ORIGIN      
        1 actggcacaa agagaagctg acaaagaagc tgctcttaaa accctggagg cttaatacct
       61 gcaagccaaa taactgaagc tcaaaacggc agaccatttg gtctgccgtt ttattatatt
      121 gacagaaaga aaatcaaacg ctgatgttta ttatttctta attctttttt tactttggaa
      181 tatggtgtat aataattagg ttatttgttt cggcagtttg ctttcacgca tgactgcccg
      241 cccattgatg gagatgatcg tatatgaaaa gactgcagga atttatgaaa aacaaaatga
      301 tgaaaagtta cagctttttt attattttca cagttgccat attatgcata atatatttta
      361 taataaagaa ctttgatctg atagccgtga ccaccggcag tgccattctc agtattcttg
      421 atgccctaac acctcttttt atcggtcttc ttctggccta tataataaac ccccttgtga
      481 acggcatcga taacagattt atgaacagga tattcaagga tcctccccct ctttccggca
      541 atgaaaaccc gactgccaaa aggaaacaca aaagaaaaaa atatcgaagc agacttgcca
      601 gtattatcct tacatatgtg attatcataa tattgatatc ccttctgatt tatcttctgg
      661 catctttact ggtaggggaa cttcttttgg aaagccttcc tgccgccgcc ggagatatag
      721 cagacaactt ggctttgtat gaaaagaacc taaggacctg ggtatcagat ctgcccggag
      781 gagtcgtctc cgaaaagata aatgatgctg ctgatgctat tctctcatgg ataagcaaaa
      841 acctttctgc cggtgccata gtaagtgcag ttacgagcgt gttcggcagc atagtcaatt
      901 ttgttatagg tcttattata agtatctatc tgcttataga caaggatttt ttcaagcacc
      961 tatggaacgg tttccttgat cttgtactgc ctcaaaaagc ctgctctgca ataaacggaa
     1021 ctcttacgga aatcaatgat gttctgtccc gcttcattcg gggggtactt atagatgcag
     1081 ttgttgttgc caccctttcc tccataggtc taacgatagc gggcctggaa ttcgcagtat
     1141 ttataggcat atttgcaggt atatgcaata tcatccctta tttcggtcct atattaggca
     1201 tgatacccgc atttcttgtg ggattcatta cagagggtct ttggcagggc gtgattgccg
     1261 ttatcatcct tcttgtaata cagcagatag acggcaacct catatatcca aaggttgtgg
     1321 gttcatccac cggacttcac cctctttttg tccttttggc ggtaagcata ggcagttact
     1381 atgccggtat agcaggtatg gtcctggcag tgcctgttgc gggagttctc cagatattct
     1441 gctatagatg gatagccttc cagcaaaaaa gaaaagaaga cagaaatcag gaaggcggga
     1501 atgaccgtga acgggaacag gagacagata ttaagcaaac tgtaaaaaaa ccataaagaa
     1561 agatattaaa aaagaagaaa aaatcgttca tagaataaac agtaaaaaag gactcttatt
     1621 aagagtcctt tcgtttctta tgatatcaga caagctccag aaaaaccact tctgctgagt
     1681 caccctgtct tgggcccagc ttcagtatcc ttgtataacc gccgtttctg tcggcatatt
     1741 tcggtgctat atcatcaaat aattttttta caacgtcttc gtcatataca tatgccatca
     1801 cctgtcttct ggcatgaagg tcaccgcgct ttgcaagggt gatcattttt tctgcctgcc
     1861 tacgggcttc cttggctctg tcatttgttg tagagatcct ttcttctctc aaaagatcgg
     1921 tcaccagatt tctcagcatc gattttctgt gtgctgaatc tctacccagt tttttatagc
     1981 ctggcatttt gctgcctcct ttcatctatt cgtcggtttc acgaagtcca agacccagtt
     2041 cttccagttt aagtttaact tcatcaaggg atttctttcc gagatttcta actttcatca
     2101 tatcgtcttc cgatttatgg gtcagctctt ctactgtatt tattgccgct ctcttgagac
     2161 aattgaatga cctgacagaa agttcaagtt cttctatcgt catttccaga gccttctctt
     2221 tctgatcttc ttctttctcg accattattt ccatggattc tgtgtcatct gtaagctgta
     2281 cgaacagatt gagatgctcc tgcattattt tggctccgat ggaaactccc tcttcagggg
     2341 aaatggatcc gtcggtccat atttcaagta tcagtttatc ataatctgtt acctgcccta
     2401 cccttgtatt ctccacggta aagtttacct ttacaacggg tgtaaaaatg gaatcaacgg
     2461 gaataacagc gataggcgta ctctcagtct tgttctgttc tgcgggcaca tatcctctgc
     2521 cgtttgccag attgatctcc attaccaggg atgcattttc ttccagggtg gcgatatgaa
     2581 gacccggatt gaatatttcc atatcggcat ctgcgattat gtctgcagca gtcacttctt
     2641 ttggcccaac tgcatttatt atcactcttt tt
//
LOCUS       DAONHK010000143         2672 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_32149_length_2672_cov_4.632021, whole genome shotgun sequence.
ACCESSION   DAONHK010000143 DAONHK010000000
VERSION     DAONHK010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32149_length_2672_cov_4.632021"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..746)
                     /locus_tag="PLM92_05270"
     CDS             complement(<1..746)
                     /locus_tag="PLM92_05270"
                     /inference="COORDINATES: protein motif:HMM:NF015042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-specific 2-thiouridylase"
                     /protein_id="HPR24581.1"
                     /translation="MNTNSRKVAAAVAENKVVLGLSGGVDSTAAALLLKEKGFEVTGL
                     YFDVTTEADEKSEKSRAAAEKAAKEMDIDFVYKNVSREFNEIVLSNFCDEYLSGRTPN
                     PCIICNPLIKFKTLKETADAKGARYISTGHYASVVFREDVYYIKTAANKKKDQSYMLY
                     RLPQEILSGLILPLGDFESKDDIRKFVKENDLSNADSPDSQEICFIDSDEGYAEFFRK
                     NGYAAERGDFIDMKGDIIGSHKGIINYTVG"
     gene            complement(700..1164)
                     /gene="nifU"
                     /locus_tag="PLM92_05275"
     CDS             complement(700..1164)
                     /gene="nifU"
                     /locus_tag="PLM92_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005837789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HPR24582.1"
                     /translation="MGMYNDKVMDHFMNPHNVGELENPDGVGKYGSPVCGDMMQIMIK
                     VSDDKITDAKFKTFGCGSAIASSSIATDMIIGKTIDEALEMTNKQIVDELGGLPAVKI
                     HCSVLADHAIKAAIYDYAKKNNKEYEGLKGFDPNAEEDEHEQPEGCSGGCGK"
     gene            complement(1169..2338)
                     /gene="nifS"
                     /locus_tag="PLM92_05280"
     CDS             complement(1169..2338)
                     /gene="nifS"
                     /locus_tag="PLM92_05280"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005837788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HPR24583.1"
                     /translation="MKEVYLDYSATTPVKKEVFEEMAPYFTEHFGNPSSLYKIASESK
                     EALNLARKRLADLINASPEEIFFTGSGTEADNWAVFGTLDAMKKKGNHMITSSIEHHA
                     IMHTAQYLMKRGIDATFLSVDKEGIISPAELEKSINDKTVLVSLMMVNNEVGSIEPIK
                     ELALVAKKHGVLFHTDAVQALGNVPIDVKELGVDMMSLSSHKIYGPKGVGALYIRKGV
                     RISNFVHGGAQENKKRAGTENLAGIVGFGKAAELANKNLTDHINRLTELRNYFIDRVK
                     SSIKDVNINGSMDHRHPGNANITFNYIEGEAILLLLNEKGIYVSTGSACSSASLSPSH
                     VLAAMGVPVEMIHGTIRFTMGDFTTKEDLDYTVEALKEIITKLREVSSVSEEKGW"
     gene            complement(2394..>2672)
                     /locus_tag="PLM92_05285"
     CDS             complement(2394..>2672)
                     /locus_tag="PLM92_05285"
                     /inference="COORDINATES: protein motif:HMM:NF017762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HPR24584.1"
                     /translation="FDTASKSDYNIIVGFFVKGTYMISEEKIKRINQLAKKQKEGELT
                     PEEKKEQKILRDEYIAAIRQRTKQHLDRIRFVEDMSEEELDSIKKKEN"
BASE COUNT          606 a          650 c          479 g          937 t
ORIGIN      
        1 tgtcccacag tatagtttat tatgccttta tggctgccga ttatgtctcc cttcatatca
       61 atgaaatcgc ccctttcggc ggcataaccg tttttcctga aaaactcagc atatccttca
      121 tcgctgtcaa taaaacatat ctcctggctg tcgggcgaat cagcattgct tagatcattt
      181 tcttttacga attttcttat atcatccttg ctttcaaaat cgcccagagg caaaatcagt
      241 ccggacagga tctcttgggg cagtctgtaa agcatgtagg actgatcctt ttttttgttt
      301 gctgccgttt ttatataata tacatcttcc cgaaaaacga cggaggcata gtggcccgtt
      361 gaaatataac gggcaccctt cgcatctgca gtctccttca gtgttttgaa ttttatgagg
      421 ggattgcata ttatacaggg attcggtgta cggcccgaaa gatattcatc gcagaaattg
      481 gaaagcacaa tttcattgaa ctcccgggac acattcttat aaacaaaatc tatatccatt
      541 tcttttgccg ctttttctgc tgctgccctg cttttttcac tcttttcatc cgcttctgtg
      601 gttacatcaa aataaagacc tgttacttca aagccttttt ctttcaataa taaagcagcg
      661 gcagtgctgt caactccgcc gctcaatccc aaaactactt tattttccgc aaccgccgct
      721 gcaaccttcc ggctgttcgt gttcatcttc ttctgcattt ggatcgaaac ccttcagccc
      781 ttcatattct ttgttgtttt tcttggcata atcatatatc gcagctttta tggcatgatc
      841 ggccagtacc gagcagtgta ttttcacagc gggaagcccg cccagctcat cgactatctg
      901 cttattggtc atttccagag cttcgtctat ggttttgcct attatcatat ctgtagcgat
      961 acttgatgat gctatggcag agccgcatcc gaaggtcttg aatttagcat cggttatctt
     1021 atcatcgctg acctttatca ttatctgcat catgtctccg catacagggc tgccgtactt
     1081 gcccaccccg tcggggtttt caagttcgcc cacattatga ggattcatga agtgatccat
     1141 taccttgtcg ttatacattc ccattttttt accagccttt ctcctccgag acagaggata
     1201 cctctctgag tttagttatt atttctttaa gtgcttccac tgtataatcc agatcttcct
     1261 ttgttgtaaa gtctcccatg gtgaatctta tggtaccgtg tatcatctca acgggcactc
     1321 ccatggccgc cagtacatgg gaaggcgaaa gggatgccga ggaacaggcg gatcctgttg
     1381 aaacatatat ccccttctca ttgagcaaca gaagtatggc ttctccctcg atataattga
     1441 aggttatatt tgcattcccc gggtgtctgt gatccatgct cccgtttata ttcacatcct
     1501 ttatgctgct tttgactctg tcgataaaat aatttctgag ttctgtgagt ctatttatat
     1561 gatccgtcaa atttttattt gccaactctg ccgctttccc gaatcctact atgccggcaa
     1621 gattttctgt ccccgcacgc ttcttgtttt cctgagcgcc gccgtgcacg aaatttgata
     1681 tccttacgcc ctttcttata tagagagctc ccacaccctt tggtccgtat atcttatggg
     1741 aggaaaggga catcatatcg actcccagtt ccttgacatc tattggcaca ttccccaggg
     1801 cctgcaccgc atccgtatgg aaaagcactc cgtgtttctt ggccacaagg gccagttcct
     1861 ttatgggctc tatgctgccc acttcattat tgaccatcat cagtgaaaca agaacagttt
     1921 tgtcatttat ggacttttcc agttccgccg ggcttattat gccctccttg tccaccgaaa
     1981 gaaatgtggc atctattcct cttttcataa gatactgggc agtatgcatg atagcatgat
     2041 gctcgatact gctggttatc atatggtttc cttttttctt cattgcatcc agtgtgccga
     2101 aaacagccca gttgtcagct tctgtaccgc ttcccgtaaa gaatatctct tcgggggaag
     2161 catttataag atctgccagt ctttttctgg caagattcag tgcctctttt gattctgatg
     2221 ctattttata taaacttgaa ggatttccga agtgttccgt aaaatacggt gccatttctt
     2281 caaaaacttc tttttttacc ggtgttgtcg ccgaataatc aagatatact tctttcattt
     2341 tcagatgtgc tcctttactt tttctttata ccaatttctg ggtattttca aactcaattt
     2401 tctttctttt taatagaatc aagttcttct tctgacatat cctcaacaaa acggatccta
     2461 tccagatgtt gttttgttct ttgtcttatt gccgcaatat attcatctct caagatcttc
     2521 tgctcttttt tttcttccgg agtaagttcc ccttctttct gcttttttgc cagttggttt
     2581 attcgtttta tcttttcttc tgaaatcata tatgttcctt tcacaaaaaa tccgactata
     2641 atattatagt cggatttact ggctgtgtca aa
//
LOCUS       DAONHK010000144         2670 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_32203_length_2670_cov_2.681836, whole genome shotgun sequence.
ACCESSION   DAONHK010000144 DAONHK010000000
VERSION     DAONHK010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2670
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32203_length_2670_cov_2.681836"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="PLM92_05290"
     CDS             complement(<1..293)
                     /locus_tag="PLM92_05290"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPR24585.1"
                     /translation="MKSIMKFFFVFIIIVFIAVLIGAGILAYAFFVEPQRLSGTELRM
                     SNSNITESLSVAVFADTHFGKNYDISDFKKATDEINKASPDVIIFLGDLIDDY"
     gene            412..1617
                     /locus_tag="PLM92_05295"
     CDS             412..1617
                     /locus_tag="PLM92_05295"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacetone oxidase family FAD-binding enzyme"
                     /protein_id="HPR24586.1"
                     /translation="MKKNDTDICIIGCGASGMAAAISAAQMSPSKDICIIEKKEIPGK
                     KLYATGNGRCNISNRACMDSDDVMSFFESIGVALREEEEGRIYPLSGQAADVVLALEK
                     AMEVYNINIIVNSTVTDVKKEKDCFIITYKEKKTGEESTVNSKKLLIACGGKAGPQYG
                     TSGDGYSISKKLGHNASATFPILTPLECEGDFSLLKGVRTPAKAVLMRKNKKAAQETG
                     RIQFTDDGLSGICIFDLSRYIRTEEDVQWRESMAEYKIVLDFFPEKSEKKLINELTKR
                     KEIKGFKAEDLLISYLNRPLAAEILKNNGADTAKPASAVTEKEIKELAASMKSLCFRI
                     KGKKGWKVAQCTAGGIKLEEINSDTMESKITKGLYFAGEILDHDGPCGGYNLHNAWKT
                     GIKAGKAMV"
     gene            1618..>2670
                     /locus_tag="PLM92_05300"
     CDS             1618..>2670
                     /locus_tag="PLM92_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(FAD)-utilizing dehydrogenase"
                     /protein_id="HPR24587.1"
                     /translation="MYRIHQIILRLGEDKDSIPSKIAKKTRNSEKSIKEWKIVKESVD
                     AREKNDVKMVYTVDFSCEGKANAEKAPDMTYRPVDPTGTHPGKRPVITGSGPCGMFAA
                     LILSEAGFAPLVIERGRSVEDRIKDVKGFKEKGILDEESNVQFGEGGAGTFSDGKLYS
                     GIKDKRIRKVLETFYEAGAGHEILYKQKPHIGTDRLRLILPEIRSRIIKNGGEIRFSS
                     KLTEIHTENGKLKYISVNDEEEIETDDLILAQGQSAYDTISMLYKKGLIMEQKAFSIG
                     IRIQHEQRMIDEAQWGSSEKSILLGPAEYRLSGRCRNKRGVYTFCMCPGGEVIPAASS
                     NGHTVINGMSNSSRDGK"
BASE COUNT          964 a          474 c          626 g          606 t
ORIGIN      
        1 ctgtaatcat caatgagatc gcccaaaaaa atgatgacat ccggagaagc tttgtttatt
       61 tcatctgtag cttttttgaa atcggatata tcataattct ttccgaaatg ggtatcggca
      121 aaaactgcaa cggacaggga ctctgttatg ttggaattac tcattcttag ttccgttcct
      181 gagagtcttt gcggttccac aaagaatgca taggccaaaa tgccggcacc gatgagaacg
      241 gcaataaaaa ctattattat aaaaacaaaa aagaatttca ttattgattt caatcgctaa
      301 acctcctgac tgaactatga taacattgaa accatagcaa atcaatgatg agataatgag
      361 tttgacgact ttgtagagat tagaaaaaaa ttgtgataca atcgatatgt tatgaaaaag
      421 aatgatactg acatatgcat aatcggctgc ggagcctcgg gtatggctgc agcgatttct
      481 gcggcacaga tgtcgccgtc aaaggatata tgcatcatag aaaaaaaaga gatccccggc
      541 aaaaaactgt atgccacagg taacgggaga tgtaacatat caaacagagc atgcatggac
      601 tctgatgatg tgatgtcttt ttttgagtct atcggggtag cccttagaga ggaagaggaa
      661 gggcgcatat atcctttatc cggtcaggca gcagacgtgg ttcttgcttt ggaaaaggcc
      721 atggaagttt acaatataaa tattattgta aatagtacag tcactgatgt aaaaaaagaa
      781 aaagattgtt ttataataac atataaagaa aagaaaacag gtgaagaaag tactgtaaac
      841 agcaaaaaac ttcttatagc ctgcggaggc aaagcgggac cccagtacgg tacctccggg
      901 gacggataca gtatttctaa aaaacttgga cataatgcat ctgccacttt tccaatactg
      961 actccgctgg agtgtgaagg ggatttcagt cttttaaaag gtgtgcgcac acctgcaaaa
     1021 gccgttttga tgcgaaaaaa caaaaaagca gcacaggaaa caggcaggat ccagtttacc
     1081 gatgacggtc tctccgggat atgcattttc gatctgagca gatacataag aacagaagaa
     1141 gatgtccaat ggagggaaag catggctgaa tataagatag tgcttgattt ttttcctgaa
     1201 aaaagcgaaa aaaaacttat taatgaactt acaaagcgaa aagagataaa agggttcaag
     1261 gctgaagatc tgctgatttc atatttaaac agaccccttg cagctgaaat actgaaaaat
     1321 aacggagcgg atactgcaaa accggcctcg gcagtgaccg aaaaagaaat aaaagaactg
     1381 gccgcatcaa tgaaatcttt atgcttccgg ataaaaggaa aaaaaggatg gaaagttgcc
     1441 cagtgtacag caggaggcat aaaactcgaa gaaataaaca gtgatacaat ggaatcaaag
     1501 atcaccaaag gcctttattt tgcaggagaa atactggatc atgacggacc ttgcggaggg
     1561 tacaaccttc ataatgcatg gaaaaccgga ataaaggcag gaaaggcaat ggtatagatg
     1621 tacaggatac atcagatcat tttgcggctc ggcgaggata aggactcaat accttcaaag
     1681 atcgcaaaaa aaacaagaaa tagtgagaaa tcaataaaag aatggaaaat agtaaaagaa
     1741 tccgttgatg ccagggagaa aaatgatgta aaaatggtat acacggtaga tttttcatgt
     1801 gaagggaaag caaatgccga aaaagctcct gatatgacat acagaccggt agatccgaca
     1861 gggacacatc ccgggaaaag gcctgttata acaggatcgg ggccctgtgg catgtttgct
     1921 gcccttatac tttccgaagc cggttttgca cctttagtga ttgaaagagg ccgcagtgta
     1981 gaagaccgca taaaagatgt aaagggtttc aaagaaaaag gcatcctgga tgaagaatcc
     2041 aacgttcagt tcggagaagg gggagcgggc acattttccg acggaaaact ttattcgggg
     2101 ataaaggata aacgcatcag aaaagtcctg gaaacctttt atgaagcagg ggctggtcac
     2161 gaaatattat acaaacagaa accccatatc ggaacggaca gactcagact tatattaccg
     2221 gaaatacgct cgagaataat aaaaaacggc ggagaaataa gattttccag caaactgacc
     2281 gaaatacaca cggaaaacgg gaaactgaaa tatatttctg tgaacgatga agaagaaata
     2341 gaaacggatg atctgatcct ggcgcaagga caaagtgcat acgatactat aagcatgttg
     2401 tacaaaaaag gcctgatcat ggagcagaag gctttttcca tcggtatcag gatccagcat
     2461 gaacaaagga tgatcgacga agcccagtgg ggcagttccg aaaagtcaat tcttctgggt
     2521 ccggcagaat acaggctttc cggaagatgc aggaacaaaa gaggcgtata tactttctgc
     2581 atgtgtcccg gaggagaagt tatacctgca gcatcctcaa atggacatac agttataaat
     2641 ggcatgagta acagttcgag agacgggaaa
//
LOCUS       DAONHK010000145         2664 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_32308_length_2664_cov_3.094289, whole genome shotgun sequence.
ACCESSION   DAONHK010000145 DAONHK010000000
VERSION     DAONHK010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32308_length_2664_cov_3.094289"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            127..894
                     /locus_tag="PLM92_05305"
     CDS             127..894
                     /locus_tag="PLM92_05305"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018782910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HPR24588.1"
                     /translation="MKLKGKTAIVTGASSGMGRDIASYFAKEGANVLVVARRAERLKD
                     LEEQTKELEGKVVAFSADVSDKSKAEAMIDEAVKVFGKLDILVNNAGIMDDMSGIGDM
                     GDEMYDKVFDVNVKGTVYAMRKAVKYFEGVGGGNIINIASIGGIKGGIAGAAYTASKH
                     AVVGLTKNTAYMYAPKNIRCNAICPGAIETEIASSEYMKKINMEGAQRTGVGLALNPR
                     SGKGSEIATVAVFLASEDASFVNGQCLAVDGGWTAYM"
     gene            1062..>2664
                     /gene="katG"
                     /locus_tag="PLM92_05310"
     CDS             1062..>2664
                     /gene="katG"
                     /locus_tag="PLM92_05310"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018213251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HPR24589.1"
                     /translation="MEERKCPVTGKTAKSVSGGGTENKDWWPDKLNLKMLHQNSSLSN
                     PMGSDFKYSEEFKKLDLKEVKNDLYDLMTDSKDWWPADYGHYGPLFIRMAWHSAGTYR
                     MGDGRGGASDGTQRFAPLNSWPDNVNLDKARRLLWPIKKKYGKKISWADLMILAGNCA
                     LESMGCKTFGFGGGREDVWEPQEDVYWGSESEWLGEKRYSGERDLENPLAAVQMGLIY
                     VNPEGPDGQPSALASGKDVRETFARMAMNDEETVALIAGGHTFGKCHGAGPATHVGKE
                     PEAAPMEEMGLGWKSTYKSGKGPDTIGSGIEGAWKPGPTKWDMGYLETLFKYDWDLVK
                     SPAGAWQWIPADPEARKTVEDAHDPNKTHAPMMTTADLSLKMDPIFRPIAKKYLDHPE
                     EFADAFGRAWFKLTHRDMGPRSRYLGPEVPEEDLIWQDPLPEADYKMIDEQDIVYLKK
                     IIMASGLSVSQLVSVAWASASTFRGSDKRGGANGARIRLAPQKDWEVNQPDILNTVIK
                     TFEKIKTDFNNAQTENKKVSLADIIV"
BASE COUNT          882 a          496 c          699 g          587 t
ORIGIN      
        1 gaataaaaaa tatgtttgga actaatgaaa atgggtaaaa atttaacaat ccatttaatg
       61 cagacaatca tccaaaaaga aaaaagacaa ttacatgtgt gaagtgatat tgaaaaggag
      121 aacatcatga aattaaaagg aaaaacagct atagtgaccg gagccagctc cgggatggga
      181 agagatatag catcatattt tgcaaaagaa ggagcaaacg tgctggttgt ggccagaaga
      241 gctgaaagac taaaagatct tgaggaacag acaaaggaac tggaaggaaa agttgttgca
      301 ttttctgcag acgtttctga caagagcaaa gcggaggcca tgatagatga agcagtgaaa
      361 gtattcggca agctggacat acttgtaaac aatgcgggaa taatggatga catgagcggc
      421 attggtgata tgggcgatga gatgtacgat aaggtatttg atgttaatgt caaaggcaca
      481 gtgtatgcca tgcgcaaagc agtcaaatat tttgaaggcg tcggaggcgg aaatataata
      541 aatatcgctt ccataggcgg tatcaagggg ggcatagcag gcgcagcata tacagcaagc
      601 aaacatgcgg ttgtgggact tacaaaaaac actgcctata tgtatgcacc aaagaatata
      661 agatgtaatg ccatatgccc gggagccatc gagacagaga tagcaagtag cgaatacatg
      721 aagaaaataa acatggaagg ggcacaaagg accggtgttg gactggcact taatccaaga
      781 agcggaaagg gcagtgaaat agcaacggta gccgttttcc tggccagtga agatgcatca
      841 tttgtaaacg ggcagtgcct tgcagtagac ggcggctgga cggcatatat gtaaaccaat
      901 agataatgta aagagacaaa aatcaacaga aaaaaaagag gcagatcgtt atcatatcgc
      961 agacgtgatt tgttatgatc atgcctcttt gtttacactt ttggctgtgc tgtgttatat
     1021 ttaagatgta taagccaact ttgcgaaagg aggactacat tatggaagag aggaaatgtc
     1081 ccgtaacagg gaaaacggca aagtctgtat caggcggcgg cacagaaaac aaagactggt
     1141 ggcctgataa gcttaactta aagatgcttc accagaactc atctttgagt aatcccatgg
     1201 gatctgattt taaatattca gaggaattca aaaaactgga tctgaaagag gtcaaaaatg
     1261 acctttatga tcttatgaca gattcaaaag actggtggcc ggccgactat ggtcattacg
     1321 gccccctttt tatcagaatg gcatggcaca gtgcaggaac ctaccgaatg ggagacggac
     1381 ggggaggcgc ctctgacgga actcagcgat ttgcacctct taacagctgg cccgacaatg
     1441 tgaacctgga taaggcccgc cgtttgctgt ggcccataaa aaagaaatac ggcaaaaaaa
     1501 tatcatgggc ggatcttatg atactggcag ggaattgtgc tcttgaatcc atggggtgca
     1561 aaaccttcgg gtttggtggc ggccgtgaag atgtatggga acctcaggaa gatgtttact
     1621 gggggtcaga aagtgaatgg ctgggagaaa aaagatattc aggtgaaaga gaccttgaaa
     1681 atccactggc agcagttcag atggggctta tatatgtcaa tccggaaggc cctgacggtc
     1741 agcccagtgc tcttgcatcc ggcaaggatg tccgggagac ttttgcaaga atggctatga
     1801 atgatgaaga aacagttgct ctgattgcag gaggacatac cttcggaaaa tgtcacggag
     1861 cggggcctgc aacacatgtg ggaaaagaac cggaggctgc acccatggaa gaaatgggac
     1921 ttggatggaa gagtacatac aaaagtggaa aaggtccgga taccatcgga agcggaattg
     1981 aaggggcatg gaaacccgga cccacaaagt gggatatggg atatctggag acacttttta
     2041 aatatgactg ggatcttgtg aaaagccctg caggagcatg gcagtggata cctgccgatc
     2101 cggaggcgcg aaaaactgta gaagatgctc atgatcccaa taagactcac gcacccatga
     2161 tgacaaccgc agacctttcc ctcaaaatgg atcctatatt ccgacctata gcaaaaaaat
     2221 atcttgatca tcccgaagag tttgcagatg cctttggcag agcatggttt aaacttacac
     2281 atcgtgatat gggaccgaga tcccgttacc tgggtccgga agtccctgaa gaagatctta
     2341 tatggcagga tcctttgcct gaagcagatt acaagatgat agatgaacag gacattgtat
     2401 accttaaaaa aataatcatg gcttcaggtc tttcagtttc acagcttgta tctgtcgcat
     2461 gggcttcggc atcgactttc cggggttccg acaagcgcgg cggtgcgaac ggagcgcgta
     2521 taagactggc tccgcaaaaa gactgggagg tgaatcagcc ggatatcctg aacacggtaa
     2581 tcaaaacatt tgaaaaaatt aaaacagatt tcaacaatgc ccaaacagaa aacaaaaaag
     2641 tttccctggc agatattatt gtgc
//
LOCUS       DAONHK010000146         2661 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_32356_length_2661_cov_3.877206, whole genome shotgun sequence.
ACCESSION   DAONHK010000146 DAONHK010000000
VERSION     DAONHK010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2661
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32356_length_2661_cov_3.877206"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..782
                     /locus_tag="PLM92_05315"
     CDS             <1..782
                     /locus_tag="PLM92_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HPR24590.1"
                     /translation="AVNVSIALKKPLLIKGEPGTGKTMLAQSISEALQKKLMIWSIKS
                     TTKAQEGLYIYDTVQRLYDSQFGEGNVADIRQYIKLGRLGEAFSADEQVVLLIDEIDK
                     ADLEFPNDLLWELDKMEFYINETKETVQAKNRPIVIITSNAEKELPDAFLRRCIFHYI
                     EFPDEDKMKEIVKVHYGDIEKKLLDNTMKAFYEIRKMNDLQKKPSTSELLDWLQALMI
                     SGVAVEKIEDQMPFIGVLLKKNQDIDIFNEIKEKGYSLKRW"
     gene            782..1975
                     /locus_tag="PLM92_05320"
     CDS             782..1975
                     /locus_tag="PLM92_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPR24591.1"
                     /translation="MFLELFYLLRARELEVSVNEWMILTEALDKGLAGSSLTGFYYLC
                     KSILVKTEADYDKLDSVFAEYFGGIKTPEDLPEEFWRWLKCDAREKDINDRKMFDSFL
                     MELDELLKNFYERIEEQKEKHDGGNYWIGTGGTSTMGTGGYHERGIRTGGKNRHRSAV
                     QIAGERNFKDFRQDTILDIRQFQMAFRKLRQYSSRIQEERSELDIEKTIDETCEKGGM
                     LHIVYDKPRKNTIKLLLLIDSDGSMLPYSRLCNRLFQAVSKSNHFKDLKVYYFHNCIY
                     DNLFRTPQCKRGDWVDTSWVLSNLDSEYKVIFVGDAAMAPLELFRRGGNSIIGLYNDE
                     PGMEWLKRFKKRYKRQIWLNPVEARSWENMYGGETLAAIREVFPMYELTLDGLEKGIK
                     KLLVK"
     gene            complement(2022..>2661)
                     /gene="lexA"
                     /locus_tag="PLM92_05325"
     CDS             complement(2022..>2661)
                     /gene="lexA"
                     /locus_tag="PLM92_05325"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006000386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HPR24592.1"
                     /translation="LKERERKILNYMKFEIKVKGYPPTVREICNALDIKSTSTAHKDI
                     ANLERSGYIVKDPSKPRALMIVDPSAKKRSESDDRYDHPEADITYVPVVGRIAAGTPI
                     LAEENLEETFPVPSRFVGSGNSFMLTVKGESMIEAGIMDGDYILVRQQETANNGDIVV
                     AMVDGFESEATVKTFYKEKDHIRLQPENPAMSPIIVEDAKILGKVKGVFRYF"
BASE COUNT          858 a          505 c          612 g          686 t
ORIGIN      
        1 atgcagtcaa tgtttccata gcactgaaaa agcctttgct aataaaagga gaaccgggaa
       61 caggtaaaac catgttggcc cagtcgattt cagaagcact tcaaaagaaa ctgatgatat
      121 ggagcataaa atcaacgaca aaggcacagg aaggactata tatttatgac acggtgcaaa
      181 gactttatga cagccagttc ggagaaggaa atgttgcgga catcaggcag tatataaaac
      241 tcggaagact tggagaagct ttttctgctg atgaacaggt agttttgctg atagatgaaa
      301 tagacaaagc cgatcttgag ttcccgaatg atctgctttg ggaactggat aaaatggagt
      361 tttatataaa tgagacaaaa gaaaccgtgc aagcgaaaaa cagaccgatt gtaataataa
      421 cttccaacgc ggaaaaagaa ctgccggatg catttttgag aagatgtatt ttccattata
      481 tagagtttcc cgatgaagac aaaatgaaag agatagtaaa ggtgcattac ggcgatattg
      541 aaaaaaaact gcttgacaat acaatgaagg cattttatga aataagaaaa atgaatgatc
      601 ttcagaagaa acccagcact tcggaactgc tggactggct tcaggcactc atgataagcg
      661 gcgtagccgt agaaaaaata gaggatcaaa tgccgtttat aggggtgctt ctaaaaaaga
      721 atcaggacat agatatattc aatgaaataa aggagaaggg ttattccctt aaacgctggt
      781 aatgtttctg gaattattct atctgctcag agcaagagaa cttgaagtgt cggtaaacga
      841 atggatgata cttacagagg ctcttgataa aggtcttgcc ggctcaagcc tgacagggtt
      901 ttattatctt tgcaaaagta tacttgtaaa gacagaggcc gactatgaca aactggattc
      961 tgtttttgca gaatatttcg gtggcataaa aacaccggaa gatctgcctg aagaattctg
     1021 gagatggctg aagtgtgatg cccgtgaaaa agacatcaat gaccggaaga tgtttgacag
     1081 ctttctgatg gaactggatg agctgctaaa gaacttttat gaaagaatag aagaacaaaa
     1141 agaaaaacat gacggcggca attactggat aggaacggga gggacttcca caatgggtac
     1201 cggcggttat catgaaagag gtataaggac aggaggcaaa aacagacaca ggtcagcggt
     1261 gcaaatcgcc ggagaaagga atttcaaaga cttccgacag gataccatac tggacatcag
     1321 acagtttcag atggcattta gaaagctcag acagtattcc tcaaggatac aggaagaaag
     1381 atccgaactg gacatagaaa agaccatcga tgagacctgt gaaaaaggtg gcatgctcca
     1441 tattgtatat gacaagccga gaaaaaacac cataaagctt ctcttactca tagactccga
     1501 cggttctatg cttccctaca gtagactttg caacagactt tttcaggccg tgagcaaatc
     1561 caatcatttt aaggatctga aggtatatta ctttcataac tgcatatatg ataatctttt
     1621 caggacaccc caatgcaaaa gaggagactg ggtagacaca agctgggtcc tgtcaaacct
     1681 tgacagcgaa tacaaggtta tattcgtggg agatgcggca atggcgcccc ttgaactttt
     1741 cagaagaggc gggaattcta ttataggact atataatgat gagcccggga tggaatggct
     1801 caaaagattc aagaaacgct ataaaagaca aatatggctt aatcccgtag aggcaagatc
     1861 gtgggaaaac atgtacggag gagaaacact ggcggccata agggaagtgt ttcccatgta
     1921 tgagctcaca cttgacggcc ttgaaaaagg cataaagaaa cttcttgtga aataaaaaag
     1981 gaccggcatc tctgaacgcg ccggtccttc tgttatttac gttaaaaata tctgaaaaca
     2041 ccttttactt tgccgagaat ttttgcgtct tcaacgataa tagggctcat ggccggattt
     2101 tcgggttgca accgtatgtg atctttttct ttgtaaaaag tctttacggt ggcttcgctt
     2161 tcgaaaccat ccaccatggc aacgacaata tctccgttgt ttgctgtctc ctgctgcctg
     2221 acaaggatat agtcgccatc cattatacct gcttcgatca tgctttcacc tttgacggta
     2281 agcataaaac tgttgccact gcccacaaat cgtgaaggaa ccgggaaagt ttcttcgaga
     2341 ttttcttcgg caagtatggg agtgccggca gctattctcc ccaccacagg aacatatgtt
     2401 atatcggctt cgggatgatc ataacgatca tcactttcgg atctcttttt tgcggaagga
     2461 tcaacgatca taagtgccct gggtttggac gggtccttta ctatatatcc ggatctctcc
     2521 agattggcta tgtctttatg tgctgtagaa gtcgatttga tatcaagagc attgcatatc
     2581 tccctcacgg tgggaggata tcccttgacc ttgatctcga acttcatgta gttcagtatc
     2641 tttctttctc tttcttttaa t
//
LOCUS       DAONHK010000147         2642 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_32691_length_2642_cov_4.832238, whole genome shotgun sequence.
ACCESSION   DAONHK010000147 DAONHK010000000
VERSION     DAONHK010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32691_length_2642_cov_4.832238"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(101..658)
                     /locus_tag="PLM92_05330"
     CDS             complement(101..658)
                     /locus_tag="PLM92_05330"
                     /inference="COORDINATES: protein motif:HMM:TIGR02175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HPR24593.1"
                     /translation="MRINVRFAGAGGQGVILASVLLAKAYGLGEDYNISQTQSYGPEA
                     RGGACKAEVVISDDYIDYMKVDKADVFIAFNQVGFNKYKDTIKKDAMVLINSTLVESD
                     DPNHYRIPATAIAEDMEKPFVINMIMRGALTKLMPKLHYPAVEEEVMDNFSASIAGIN
                     LMAYEKGYDEMEKELEEREKNNTKI"
     gene            complement(655..879)
                     /locus_tag="PLM92_05335"
     CDS             complement(655..879)
                     /locus_tag="PLM92_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004034245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPR24594.1"
                     /translation="MADITIDKACCKGCDICLSICPKKIFVRSKKRNNYGTNMPEAVN
                     RDTCVFCGMCERLCPDGAINVRKTEEDKEK"
     gene            complement(872..1696)
                     /locus_tag="PLM92_05340"
     CDS             complement(872..1696)
                     /locus_tag="PLM92_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015791338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPR24595.1"
                     /translation="MNELYEKYIKKESLPTLFCPGCGNGIVQFAAIKAFEKMGIKDDL
                     ACVSGIGCSSWIPCYFKMDVMHTMHGRALPFAEGLKLSRPDKKILVFSGDGDCLAIGG
                     NHLIHAASRNIDLTVIEINNYIYGMTGGQKSPATPEGSVTKTSPFGSYDEPIDGCSLV
                     AAAGGTYVARWTTAHPVQLEKAIAEGIRHKGFSFIEVLTQCPVQAGKNIFGEKSPVAM
                     MARYKEMTYIYKEGMEIPEGKVRIGKIKHDTSRKEYTEKVLQKLEEKNIRKGQSNG"
     gene            complement(1693..>2642)
                     /locus_tag="PLM92_05345"
     CDS             complement(1693..>2642)
                     /locus_tag="PLM92_05345"
                     /inference="COORDINATES: protein motif:HMM:NF013974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPR24596.1"
                     /translation="SGPGYTLMQEAIGFAAVTETPTVIVNVQRGGPSTGQPTMSSQQD
                     IYQARYGSHGDYEIIVLCPSSVQEMYDFTMRAFELSEKFRTPVILLSDEVVGHMREKI
                     IMKPLPKETIEPTQIYASSPREDFFKGANSMVEGQLHDELGRRIGHIAPKSGDFINKI
                     CSKITDNIKEIADVEAFCMEDAEVAILAYGSVARSSMNAIKLARGIKDDRVRAKFRIV
                     KDLFVRGVPATIQTDYSHLKVGLIKVNTVWPFPEEKIQELARNVDMVIVPEMNTGKYC
                     REVRRALWDKTVISMPKCGGEIHSPNEILDRILEEVWCK"
BASE COUNT          591 a          662 c          527 g          862 t
ORIGIN      
        1 tttatctcgt gcgcccgcgc actcgacacg ggcttaaacg ccctattaat aaaaaaagcg
       61 gtcggggcaa tgttgctgcc gggaccgctt ttatctttcg tcatattttt gtattatttt
      121 tttctctttc ttccagttct ttttccattt catcatatcc cttttcgtag gccataagat
      181 ttattcctgc tatgctggca gagaaattat ccattacctc ctcctcaaca gcgggataat
      241 gaagtttggg cataagcttc gtaagtgctc cccgcattat catattaatg acaaagggtt
      301 tttccatatc ttcggctatg gccgtggcgg gtattctgta atggttggga tcgtcagatt
      361 caaccagtgt actgttaata agaaccattg catctttttt tatagtgtct ttgtacttgt
      421 tgaagcccac ctgattgaat gcgataaaaa catctgcctt gtcaaccttc atatagtcta
      481 tgtaatcatc tgaaatcaca acttctgctt tacaggctcc gcccctggct tcaggtccgt
      541 aactctgagt ctgagaaata ttatagtctt ctcccagtcc atatgctttg gccaaaagca
      601 cagaagcaag tatgactccc tgtcctcccg cacctgcaaa acgcacattt attctcattt
      661 ttctttatcc tcttcggtct ttcttacatt aattgcccca tccggacaca gcctttcgca
      721 cataccgcaa aacacacatg tatccctgtt gactgcctcg ggcatattcg tgccgtaatt
      781 attacgtttc ttggagcgta caaatatttt tttgggacat atggaaagac atatgtcaca
      841 acctttgcag caggctttgt ctatggttat atcagccatt gctttgccct ttccttatgt
      901 ttttttcttc gagtttttgt aaaaccttct cagtgtattc tttccgcgaa gtgtcatgct
      961 tgatttttcc tatccttacc ttcccttcgg ggatttccat gccctccttg tatatgtatg
     1021 tcatctcttt atatcttgcc atcatggcca ccggactttt ttctccgaaa atattttttc
     1081 cggcctggac gggacattgg gtcagtacct ctataaaaga gaaaccttta tgccggatgc
     1141 cttctgcaat ggctttttcc agttgcaccg gatgggctgt ggtccatctg gcaacatagg
     1201 tccctccggc ggcggccaca agactgcacc cgtctatggg ttcatcgtaa ctgccgaatg
     1261 gagaggtctt tgtgaccgaa ccctctggtg tggccgggga tttctgcccg cctgtcatac
     1321 cgtaaatata attatttatt tctatcacag taaggtctat atttcttgac gcagcgtgta
     1381 tcaaatgatt accgcctatt gcaaggcagt ctccgtctcc cgaaaaaacc aggatctttt
     1441 tatcaggcct ggaaagttta agcccttcgg caaagggaag cgctcttccg tgcatggtat
     1501 gcattacatc catcttgaag taacagggta tccaggaaga acagcctatc ccgctcacac
     1561 atgccaggtc atcttttatg cccatcttct caaaggcctt gatggcggca aactgaacga
     1621 ttccgttgcc gcatccggga cagaaaagtg tgggaagtga ttctttcttg atatattttt
     1681 cataaagttc gttcacttgc accaaacctc ctcaagtatc ctgtccagaa tttcatttgg
     1741 ggaatgtatc tcccctccac acttgggcat ggatattact gttttgtccc aaagagctct
     1801 ccgcacttcc ctgcaatatt ttcctgtatt catttcgggc actatgacca tatccacatt
     1861 tcttgccagt tcctgtatct tttcttcggg aaagggccag actgtgttta ccttgatcag
     1921 tccgactttc agatggctgt aatccgtttg tatggtggca gggactcctc tgacaaacag
     1981 atcctttact atacggaatt ttgctcttac cctgtcatcc tttatacctc ttgcaagctt
     2041 gatggcattc atggaagatc ttgcaacgga tccgtaggca agtatggcca cttccgcatc
     2101 ttccatgcaa aaggcctcca catccgctat ttcttttatg ttatctgtga tctttgagca
     2161 tattttattt atgaaatcac cgcttttggg ggcgatgtgc cctattcttc tcccaagttc
     2221 atcatggagt tgtccctcga ccatggagtt tgcacccttg aaaaaatcct ctctgggtga
     2281 agacgcatag atttgtgtcg gttcaattgt ttcttttggc aggggcttca taatgatctt
     2341 ttctctcatg tgtcccacaa cttcatcgga aagcagtatg accggcgtcc tgaatttctc
     2401 ggaaagctca aatgccctca ttgtaaaatc atacatctcc tgtaccgaag aaggacaaag
     2461 cactattatt tcataatctc cgtggctgcc gtatctggcc tgatatatgt cctgttggga
     2521 tgacatggtg ggctgtcctg tggaggggcc tcctctttgc acatttacga tcaccgtggg
     2581 tgtttctgta accgctgcaa atcctatggc ctcctgcata agcgtatatc cgggacccga
     2641 tg
//
LOCUS       DAONHK010000148         2614 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_33210_length_2614_cov_3.864009, whole genome shotgun sequence.
ACCESSION   DAONHK010000148 DAONHK010000000
VERSION     DAONHK010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33210_length_2614_cov_3.864009"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..821
                     /locus_tag="PLM92_05350"
     CDS             <1..821
                     /locus_tag="PLM92_05350"
                     /inference="COORDINATES: protein motif:HMM:NF019342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HPR24597.1"
                     /translation="AVSKSCILLFSIMLVTVLCFGCGDNEEADVQETEKAEIGISFME
                     DTESGEYSEDLQVYIDAIEKADAEPVLLPLIENEEDAEEALDDVDALVLTGGEDIDPS
                     YYDEEPDENLEDVNEERDTSDFLLLDEALDEDLPVLATCRGMQVLNVACGGSLYQDIP
                     TQYDTDMLHRSVDQVEFVWHNINIDRGSLLAGIMSDGDLKVNSWHHQGVKDLGQGLKV
                     VAVADDGMIEAIEKEDETFVLGVQFHPEWHIDYGDKEFLAFFEILADQAENGND"
     gene            complement(837..1457)
                     /locus_tag="PLM92_05355"
     CDS             complement(837..1457)
                     /locus_tag="PLM92_05355"
                     /inference="COORDINATES: protein motif:HMM:NF012696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HPR24598.1"
                     /translation="MTNKKFGISEALDLLKKGNERFVKGLESPGDISFQRRKDLAENG
                     QSPYAAVLTCSDSRTPPEHIFNAGLGDIFVVRNAGNLAGDCDIGSIEYAAEHLGVPVV
                     VVLGHDKCGAVSSAVDIFLSGEELPGNDPLSAMLNEICENACAKAGADITREEFIKRS
                     EDLNIEHSADKLQKSPIIADLVKKGTLVVVRAKYSLETGQVTFFDR"
     gene            complement(1740..2450)
                     /locus_tag="PLM92_05360"
     CDS             complement(1740..2450)
                     /locus_tag="PLM92_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014281354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HPR24599.1"
                     /translation="MKYKLAHKYDKYMDKMMGPNPIKLQEELLEGHNIPKGATVMDLG
                     SGQGLTSVFLVKEYGFRVFATDLWSDPTDNMEFFRSTGLDTDKIIPIHADANDLPYAK
                     DFFDAVVSTDSYNYFGREKDYLGQKLLPYVKRGGYIYISVPGMKKDCHDNLPKELLLS
                     WTPEQMDYIHDALYWERLIRQTEGVDILSIHEMESNEEAWADWLACDNEYAVEDRAAM
                     EAGGGRYMNFIGMILKRQ"
BASE COUNT          700 a          578 c          570 g          766 t
ORIGIN      
        1 aagctgtttc aaaatcatgt attcttttgt tttccattat gctggtgacc gtcctttgtt
       61 tcggatgcgg cgataatgaa gaagccgatg tccaggaaac ggaaaaggcc gaaataggca
      121 tctcctttat ggaagacact gaatcaggag aatactcaga ggatctgcag gtatatatcg
      181 atgcaataga aaaagccgat gcagaacctg ttctgcttcc tcttatcgag aatgaagaag
      241 atgcagaaga agcccttgat gatgtggatg ccctggtatt aacaggagga gaagatatcg
      301 acccctcata ctatgatgaa gaaccggatg aaaaccttga ggatgtcaat gaagaaagag
      361 acacatccga ctttttgctg cttgatgaag ctcttgatga agatctcccc gttctggcca
      421 cctgccgcgg aatgcaggta ctcaatgttg cctgcggagg cagtctttat caggatatac
      481 cgacccagta cgatacggac atgcttcaca gatctgtgga tcaggtggaa tttgtatggc
      541 ataacataaa tatcgacaga ggttctctgc tggcgggcat catgagtgac ggagatctga
      601 aagtcaactc atggcatcat cagggagtca aggatctggg ccaaggcctt aaggtagttg
      661 ccgtggccga tgacggtatg atagaagcca tcgaaaaaga agatgaaacc ttcgtcctgg
      721 gagtccagtt ccatccggaa tggcatatag attacggcga taaagaattt ctggcgtttt
      781 tcgagatcct ggcagatcaa gctgagaacg gaaacgactg atgatcttgt aaatatctac
      841 ctgtcgaaaa atgtcacttg gcctgtttca aggctgtatt ttgccctgac tacaacaaga
      901 gtgccttttt tgacaagatc ggcgatgatg ggactttttt gcagtttatc tgccgagtgt
      961 tctatgttta gatcttcgga tctttttatg aactcctccc gagtgatatc agcccctgct
     1021 tttgcacagg cattttcgca gatctcatta agcatggcag aaaggggatc attgcccggc
     1081 aactcttcgc ccgaaagaaa aatatcaacg gccgaactga ctgctccgca tttgtcatgt
     1141 cccagtacaa caactacagg aacgccaagg tgctctgcgg catactcgat gctgcctata
     1201 tcacagtccc ctgccagatt gcccgcattc ctgactacaa aaatgtctcc cagacccgca
     1261 ttaaaaatgt gttcgggagg agtccttgag tcagagcagg tgagaactgc agcataagga
     1321 gactgaccgt tttcggcgag atcttttctt ctttggaaag aaatgtcccc cggagactca
     1381 agccccttta caaatcgttc attgcctttc ttcagaagat caagtgcttc cgaaatgccg
     1441 aattttttat ttgtcattac agtgttttcc tttctgttat ttgggtatga ttatatcata
     1501 aaacttgatg gaggattaat atcatgaaaa aatttataat tttgaccatt gctgcgatcc
     1561 ttcttgtaat gaccttcact gcatgcagcg acgatgcccc gcggcccggc tgaggagact
     1621 ccgttaagtg tgcgtcggtg gcgtacgttg aaaaaagctg aaaatgaatg gccgaaatca
     1681 ataaaaagag aaatgacgga tgtcatttct ctttttgcat attgcatctt caaaaaattt
     1741 tactgtcttt ttagtatcat tccgataaaa ttcatgtacc tgccgccgcc ggcttccatg
     1801 gctgccctgt cttctacagc atattcattg tcacaggcaa gccagtcggc ccatgcttct
     1861 tcattgctct ccatttcatg tatggaaagt atatcaacac cttctgtttg tcttatcagc
     1921 ctttcccaat aaagggcatc gtgtatgtag tccatctgtt cgggcgtcca tgaaaggagc
     1981 agttcctttg gcaagttgtc gtgacagtcc tttttcattc ccgggacaga aatatatatg
     2041 tacccgcccc ttttcacata gggaagaagt ttttgaccca ggtaatcctt ctctcttccg
     2101 aagtaattat atgaatccgt gcttactacc gcatcaaaga aatcctttgc atatggaaga
     2161 tcattggcat cggcatgtat gggtattatc ttatccgtat caagaccggt gcttctgaaa
     2221 aactccatat tatctgtggg atcagaccag agatccgtgg cgaatacccg aaatccgtat
     2281 tcctttacca ggaagaccga agtaaggccc tgaccgctgc ccagatccat tacagtagct
     2341 cccttgggta tattatgtcc ttccagaagt tcctcctgaa gtttgatggg atttggcccc
     2401 atcatcttat ccatatattt gtcgtattta tgtgccagtt tatatttcat ttcattatcc
     2461 ttcctttgtt ttccatcgat catggctatt aaaagccata ttaagtgatt ttgggcacaa
     2521 aaaatgtgcc ttgttttgtt ccataatagc tctacctctt tcaaaagagt tttcaaaacc
     2581 ataagtatca gaataatttg ttggatttat atta
//
LOCUS       DAONHK010000149         2576 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_33897_length_2576_cov_3.074177, whole genome shotgun sequence.
ACCESSION   DAONHK010000149 DAONHK010000000
VERSION     DAONHK010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33897_length_2576_cov_3.074177"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1030
                     /locus_tag="PLM92_05365"
     CDS             <1..1030
                     /locus_tag="PLM92_05365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPR24600.1"
                     /translation="GCFAEGIKSEGYQTYTYSDDFSYYAAGVPSLVNGFLFEEDMETV
                     WPFYREIYHSQDDTKDTYNEKVMEFNADYYGSFAIFTDQMPALPLDFNGQYERLKKAA
                     DKKTKALMEEAGINTKAYDEALKELKTAADETKKEAEKINKGYLNAWIKDDRDTMAKY
                     KEKGAKLTERSLLAFDFAQNKFLKLMYERPIVGHEAAFENINLCRKTIEALKAGKSEE
                     AYNKYAWKINNSLEWYEMNFSPEVTRIQDDMFWGKKNKNNLFWGTGKGFVKAEVSKAT
                     RSLASKQAEIKNQNSDEIQNGNKDEKKTDFTKEIKIYEKAVKDQMEIFKDLCNREISD
                     MKELSRMI"
     gene            complement(1134..2510)
                     /gene="mgtE"
                     /locus_tag="PLM92_05370"
     CDS             complement(1134..2510)
                     /gene="mgtE"
                     /locus_tag="PLM92_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012799681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HPR24601.1"
                     /translation="MEEKKTFDQAVLEMIDLLKEKNYKKTREKLLENNEADIAEALEE
                     ILEETGIDTTIIVFRMLPKDISVEVFSRLPSDDQKEIINRITEKELTYIIDELDFDDK
                     IDVLEELPANVVDKILAKTPRNERHQINTFLNYPDNCAGTLMTPDYISLEKEMTVREA
                     LAYIKKEGMDSETIYTCYVKEGSRKLQGIVSLRTLVVSDDDTKIADIMREDFISINVY
                     EDQEEVSEMFKKYGYLAMPVVDKEQRLVGIITMDDILDVIEEENTEDIERMAGVMDSS
                     TTEYLNTGVGQHVKSRLPWLIFLMVSLMITGAIISQFEETLSQVIVLVAYLPLLMGTG
                     GNTGSQTSTLVIRSMAIGDLELKDALKVLWKEFRISITVGLILSVMNFGKVMLIDGES
                     WKIAITVSAAMLIIVAFAKVIGGMLPMAAKKIKIDPALMANPMIASITDMFSATIYLV
                     MASLILGI"
BASE COUNT          758 a          539 c          503 g          776 t
ORIGIN      
        1 aggatgtttt gctgaaggga taaaaagcga aggctatcaa acatatactt attccgatga
       61 cttttcatat tatgctgccg gcgtgccctc tctggtaaac ggttttcttt ttgaggaaga
      121 tatggagacc gtctggccct tttacaggga aatctatcat tcccaggacg acacaaaaga
      181 tacctacaat gaaaaagtaa tggagttcaa tgcggattat tacggctcct ttgctatttt
      241 taccgatcag atgcctgctc ttcctttgga tttcaatggt cagtatgaac ggctcaaaaa
      301 ggctgccgat aaaaaaacaa aagcccttat ggaagaggcc ggcataaata caaaagcata
      361 tgatgaggca ctgaaagaac tgaaaactgc cgcagatgaa acaaaaaaag aagcagagaa
      421 aataaataaa ggatatctta acgcatggat aaaagatgac agagatacca tggccaagta
      481 taaagaaaaa ggagcaaagc ttaccgaacg gagccttttg gcctttgatt ttgcccagaa
      541 caaatttctt aaattgatgt atgaacgtcc cattgtcggt catgaagctg cttttgaaaa
      601 cataaatctg tgccgaaaaa ccattgaagc cttaaaggcc ggcaaatcag aagaagcata
      661 caacaaatat gcctggaaga taaacaattc tttggaatgg tatgaaatga atttcagtcc
      721 ggaagtgacc aggatacagg atgatatgtt ctggggcaaa aagaataaaa ataatctgtt
      781 ctggggtacc ggaaagggat tcgtaaaagc tgaagtgagc aaggccacca gaagcctggc
      841 atcgaaacag gctgaaatca aaaaccaaaa cagtgatgaa atacagaacg gaaataagga
      901 cgaaaagaaa acagacttca ccaaagaaat aaaaatatat gaaaaagcag tcaaggatca
      961 aatggagatt tttaaggatc tgtgcaacag agagatctct gatatgaaag aactttcgcg
     1021 tatgatctag acggctgcta tatgacatta ttgcacgggc atatgagcgg gacttgtggt
     1081 ccgtgcacta tataagtata tgaaaagagt gccatttaaa gcactctttt ttattatata
     1141 cccagtatca gtgatgccat caccagatat atggtggctg aaaacatatc ggtaatagag
     1201 gcgatcatgg gattggccat cagagccggg tctattttta ttttttttgc cgccatgggc
     1261 aacattcccc cgatcacctt tgcaaatgcc actattataa gcatggctgc ggaaaccgtt
     1321 atcgctattt tccatgattc tccatcaatc agcattacct ttccgaaatt cataacgctc
     1381 aatataaggc caacggttat gcttatcctg aattctttcc atagtacttt cagagcatct
     1441 ttaagctcca ggtctccaat ggccatactt cttattacca gcgttgatgt ctgggaaccg
     1501 gtatttcctc ctgtccccat aagaagcggc agataggcta caagaactat tacctgggac
     1561 agcgtctctt cgaactggga tattattgct ccagttatca tcaaagaaac catgaggaat
     1621 atcagccacg gaagcctgct tttgacatgc tgccccactc ccgtattaag atactcagtt
     1681 gtggaactgt ccattacacc ggccattctc tctatatctt cggtgttctc ttcctcgatt
     1741 acatcaagga tgtcatccat ggttattatg cccaccaggc gctgttcttt atccaccacc
     1801 ggcatggcca gatatccgta ttttttgaac atctcggaaa cttcttcctg gtcttcatat
     1861 acattgatgg atatgaaatc ttctctcatg atgtcagcga tctttgtgtc gtcatcactt
     1921 actaccaggg tccttaggga gactattccc tgaagctttc tgctgccttc ctttacataa
     1981 catgtatata ttgtttcact gtccattccc tcttttttta tataagccag ggcctctctt
     2041 acagtcattt ccttttcgag acttatataa tcgggagtca tcagtgttcc ggcacaatta
     2101 tcgggatagt tgaggaaagt gttgatttga tgtctttcat tcctgggtgt ctttgcaagg
     2161 atcttgtcca caacattggc gggcagttct tcaagcacgt caatcttgtc gtcaaaatca
     2221 agctcatcaa ttatgtaggt cagctctttt tctgttatac ggtttattat ttccttctga
     2281 tcatctgaag gcaaacggga aaaaacttcc accgaaatat ctttcggcag catcctgaat
     2341 actataatag tcgtatcgat acctgtttct tcaagtattt cttccagcgc ttccgctata
     2401 tcggcttcgt tgttttccag cagtttttct cttgtttttt tgtagttttt ttctttcagc
     2461 aaatctatca tttctaacac agcctgatca aatgtctttt tttcttccat tttttttacc
     2521 tcctttcttt gcatttgatt agtatacact ttttatgtgt ttatatcaac gaaatt
//
LOCUS       DAONHK010000150         2541 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_34624_length_2541_cov_3.980290, whole genome shotgun sequence.
ACCESSION   DAONHK010000150 DAONHK010000000
VERSION     DAONHK010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2541
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34624_length_2541_cov_3.980290"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1049
                     /locus_tag="PLM92_05375"
     CDS             <1..1049
                     /locus_tag="PLM92_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPR24602.1"
                     /translation="KTKEMKQKKKTANKPDNMKRKILCFMVAFIAAFSQGVLFLTPAC
                     AETAGETLIVRVGYFGDVNDYRLKAELSREQLEALPQYTTNYTNVTRVGTIMYTVAQG
                     PKVEDVLALAGIDVWSAQTMHLRTTDATGETNNWFSEFTAAAYLSGNRFYYPNINGKW
                     ETLSESSGQALSGGTAGAQPVHTILAIRSYSTKNPEQAAVMTPAMMDEEKSYRFCTGQ
                     TPISEYVPTTMNDVSSKDSAKWIFGIDVTLYGSSEREDPNDPNDIVGSEKDSGSMKDS
                     ESTGLFAREVTLGAAVEDEEKTMKDRKEMSENAEELAAEARDPKALAIAGSVAGGALM
                     TGLFVKLILFLKEV"
     gene            1054..1695
                     /locus_tag="PLM92_05380"
     CDS             1054..1695
                     /locus_tag="PLM92_05380"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HPR24603.1"
                     /translation="MFSDTLKEVLRAVASVMQVPVMIVLLLLMVVTVIMLGSIIMEYF
                     TERRRMKAKIPSLVDRIQGKNTDELKILVEESGLLKRQKAALLKVVERKNLSDDTKDA
                     LARQIIFQEQAYYEKVSKITDVIARIAPIFGLLGTLIPLGPGLIALGQGDTKTLAASL
                     LIAFDTTIAGLISAAFAYLISSIRKRWHEEYIVALETIMETILEQQSEERGER"
     gene            1692..2006
                     /locus_tag="PLM92_05385"
     CDS             1692..2006
                     /locus_tag="PLM92_05385"
                     /inference="COORDINATES: protein motif:HMM:NF021440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2149 domain-containing protein"
                     /protein_id="HPR24604.1"
                     /translation="MRVTGKSGRLRINRKKDEINPMDGLANMADVMLVFACGLLIALV
                     VNWNVDINTLSDSDVPEKAKYEVEDIQEEASEEVTDKGRLEELGKVYRDPDTGKYYVI
                     DE"
     gene            1999..2496
                     /locus_tag="PLM92_05390"
     CDS             1999..2496
                     /locus_tag="PLM92_05390"
                     /inference="COORDINATES: protein motif:HMM:NF012401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HPR24605.1"
                     /translation="MNKRNFGKSIFVFFAGFFVILAFCGCGNPDIDISKYEDSVIVFS
                     GLTDKDIKITVKELKSMDCVTVKTKSTSDKIGEIKATGPLLDTVLDAYDAEKQDFRII
                     NIYGSDKYKISLTESFFGENDLILAFGIDEKPLEKGSRPIRLIIPGSDSAYWVRLVKK
                     IEFIR"
BASE COUNT          854 a          470 c          632 g          585 t
ORIGIN      
        1 aaaaaacaaa agaaatgaaa caaaagaaaa aaacagcaaa taaaccggat aacatgaaaa
       61 gaaagatact ttgctttatg gtcgctttta tagccgcctt ttctcagggg gttttatttt
      121 tgacgcccgc atgcgcagaa actgccggag aaactcttat agtccgtgtg ggatatttcg
      181 gcgatgtgaa tgattacaga cttaaagcgg aactttcaag ggagcagctg gaggcacttc
      241 ctcaatatac aacgaactac acaaatgtaa ccagagtagg gacaataatg tacactgtgg
      301 cacagggacc aaaagtcgaa gatgttctgg ccctggcagg gatagatgtc tggtctgcac
      361 aaaccatgca ccttagaaca acagatgcca caggagaaac aaataactgg ttttcggaat
      421 tcactgcggc ggcatatctt tccggcaaca ggttttatta tcccaatatc aacggcaagt
      481 gggaaacatt gagcgaaagc agcgggcagg cactcagtgg aggaacagca ggagcacagc
      541 cggttcatac catactggcc ataaggagct attccactaa aaatccggag caggcggcag
      601 taatgacacc cgccatgatg gatgaagaaa aaagctacag attctgtacg ggacaaacac
      661 caataagcga gtatgtgcct accaccatga atgatgtcag ttccaaggat tcggcaaaat
      721 ggatattcgg aatagatgtc actctttacg gttcttcgga aagagaagat cccaatgatc
      781 caaatgatat agtcggcagt gaaaaagatt caggaagcat gaaagacagc gaatcaaccg
      841 gtctttttgc cagagaagtg actctggggg cagccgttga agatgaagaa aaaaccatga
      901 aagacagaaa agaaatgtcc gaaaatgccg aggaactggc ggcagaagca agagatccaa
      961 aggccctggc cattgcagga agcgtggcag gaggggctct tatgaccggg ctttttgtca
     1021 aacttatatt atttttgaag gaggtatgag gacttgtttt ctgatacatt aaaagaagtg
     1081 ttaagagcag tggccagtgt tatgcaggtg ccggtaatga tagtgctgct gttgcttatg
     1141 gtagtgaccg ttataatgct gggcagcata ataatggagt attttaccga acggagacgc
     1201 atgaaagcaa agataccttc ccttgtggac cggatacagg gcaaaaatac agatgaatta
     1261 aaaatccttg tggaagaaag cggtcttctg aaaagacaga aggcagccct gctgaaagtc
     1321 gtagaaagaa aaaacctgtc tgatgataca aaggatgctt tggcaagaca gataatattc
     1381 caggagcagg catattatga aaaagtctca aagataacag atgtaatagc aaggatagct
     1441 cccatattcg gacttctggg aacacttata cctcttgggc cggggcttat cgctctgggt
     1501 cagggtgaca caaagacact ggcagcatcc cttcttatag cctttgacac aacaatagcc
     1561 ggattaataa gtgcagcttt tgcatatttg atatcatcaa taaggaaacg ctggcatgaa
     1621 gaatatatcg tagctctgga aaccataatg gaaaccatac ttgagcagca gagcgaagaa
     1681 aggggagaga gatgagagtc acaggcaaaa gcggacgact tcgcataaac agaaaaaagg
     1741 atgaaataaa tcccatggac ggtctggcca acatggcaga tgtcatgctg gtattcgcat
     1801 gcggacttct gatagcactg gtagtgaact ggaatgtgga tataaacacc ctttctgaca
     1861 gtgatgtgcc ggaaaaggcc aaatatgaag tcgaagacat acaggaagag gcttccgaag
     1921 aagtaaccga caaaggccgg ctggaagagc tggggaaagt ttacagggac cccgatacgg
     1981 gaaaatacta cgtgatagat gaataagcgg aatttcggaa aatcaatatt tgtttttttt
     2041 gcagggtttt ttgtcatact tgccttttgc ggctgcggaa atccggatat agacatttca
     2101 aaatatgagg acagtgttat cgttttttcc ggactgactg acaaggacat aaaaataaca
     2161 gtcaaggaat tgaagtcaat ggactgtgtc actgtaaaaa caaagagtac aagtgataag
     2221 ataggagaaa taaaggccac cgggccactg cttgatacgg tgcttgatgc ctacgatgca
     2281 gaaaaacagg atttcaggat catcaatata tacggctcgg ataaatacaa aatatcactg
     2341 acggaaagct tttttggaga aaatgatctt atcctggcct tcgggatcga tgaaaaacct
     2401 ttggaaaaag ggtcaaggcc cataaggctt ataatacccg gatcggattc agcttactgg
     2461 gtacgcctcg tgaaaaagat agagttcatt cgctgatgtt atttttataa aaaatgtatt
     2521 gaatttttat atggaattct a
//
LOCUS       DAONHK010000151         2525 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_34940_length_2525_cov_3.703239, whole genome shotgun sequence.
ACCESSION   DAONHK010000151 DAONHK010000000
VERSION     DAONHK010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2525
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34940_length_2525_cov_3.703239"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..229
                     /locus_tag="PLM92_05395"
     CDS             <1..229
                     /locus_tag="PLM92_05395"
                     /inference="COORDINATES: protein motif:HMM:NF014327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HPR24606.1"
                     /translation="EAEYHFVEVMACPNGCIGGGGQPRSDRDSRRKRSDALYSNDKLA
                     EIRHSAANPFVTRVYKVIVRDDAKRLLHVHY"
     gene            361..2313
                     /locus_tag="PLM92_05400"
     CDS             361..2313
                     /locus_tag="PLM92_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HPR24607.1"
                     /translation="MENKIEQQKTEKNREKPEGTRENPEGTRENTERNDREAVREKLK
                     ADLDKLLKKVEKPARYTGLEINSVMKDPDAVSVRFAFAFPDTYEIGMSYVGLQILYDI
                     LNKDKDVYCERVFAPAPDMEELLRREDMPLMSLETKTPLHQFDIIGFTLQYELSFTNI
                     LNMLDLSGIPLKREDRKQGKWPLIIAGGPCAFNPEPLSDFVDLFVIGDGEKADIDLCR
                     LYSKLKSELKKDATEDRLKKVFLEKASKMQGIYVPGRPIEGKIQKILLSDIENLSYPE
                     KPIVPFIETVHDRAVVETFRGCSRGCRFCQAGMIYRPVRERSPETIKKLAECQLKSTG
                     NDELSLLSLSISDYSGFERLVMDIMEMCQKEDVSLSLPSMRLDSFSFRVLEEIQKYKK
                     SGLTFAPEAGTQRLRDIINKNITDEDIFSSVRKALELGWQQIKLYFMVGLPGETEEDL
                     QGIADIVKKILDINYEVNGRKGGRFNVTVSVSNFVPKAHTPFQWEAQDSEESFLEKQE
                     YIRRRLPKKNVTYNYHGAYKSILEGVFARGDAECGRVLLEAWKNGCRFDSWNEHFDRQ
                     KWREAFEKTGISADKYTVRKRSYDQSLPWDFIDTYISREFLINEAKKAQIGQTTGDCR
                     EGCEDCGISRSAGCPLARGKKKGTTE"
BASE COUNT          848 a          489 c          620 g          568 t
ORIGIN      
        1 agaggccgaa tatcattttg tggaagtaat ggcctgcccc aacggatgca ttggaggcgg
       61 cgggcagccc agaagcgaca gagattccag gaggaagaga tcggatgccc tttattcaaa
      121 cgataaattg gctgaaataa ggcattctgc ggcgaatccc tttgtaacaa gggtatataa
      181 agtcatagtc agggatgatg ccaagagact tcttcacgtt cactattaga aaactaccat
      241 attttacaga cccccttaat aatctatctg aaaacggccg tttatgattt cgcggtcgtt
      301 ttcacttttt tgtaacaaac cgttacaaat gactttaaat ttgctataat atcaagcgtt
      361 atggaaaata aaatagaaca gcagaaaaca gaaaaaaacc gcgagaagcc agagggaacc
      421 cgggaaaatc cagagggaac ccgggagaat acagaaagaa atgaccggga agcagtgcgt
      481 gaaaaactga aggcagatct tgataaactt ctgaaaaaag tcgagaaacc ggcaagatac
      541 acaggtcttg aaatcaattc ggtaatgaag gacccggatg cagtatctgt tcggtttgct
      601 tttgcttttc ccgatacata tgaaataggc atgtcctatg tgggcctgca gatactttat
      661 gatatcctga acaaagataa ggatgtttac tgtgaaaggg tttttgctcc tgctccggac
      721 atggaggaac tgctgaggcg ggaagatatg cccctcatga gcctggagac gaagacgcct
      781 cttcatcagt tcgatattat aggattcact ctccagtatg agctttcctt taccaatata
      841 ctcaatatgc tggatctttc gggaataccc ttaaaaagag aagacaggaa acaggggaaa
      901 tggccactca tcatagcagg agggccctgt gctttcaacc cggagccgct ttctgacttt
      961 gttgaccttt ttgtcatagg ggacggtgag aaggcggata ttgatctttg cagactttat
     1021 tccaaactca aatctgaact caaaaaagat gcaactgaag accggctgaa gaaggttttc
     1081 cttgaaaaag catcaaaaat gcagggtata tatgtgccgg gcaggccaat tgagggcaaa
     1141 atacaaaaga tattgctttc cgatattgaa aatctttctt atccggaaaa acccatagtg
     1201 cctttcatag aaactgtcca cgacagggcg gttgttgaga ctttcagggg atgcagcagg
     1261 ggatgcaggt tctgccaggc gggcatgata tacaggcctg tcagagaaag aagccccgaa
     1321 accatcaaaa aactggcgga atgtcagtta aaaagcaccg gaaacgatga actttcactt
     1381 ctttcccttt ccatctccga ttattcggga ttcgagcgac tggtaatgga tatcatggaa
     1441 atgtgccaaa aagaagacgt ttctctttcc ctgccttcca tgagactgga ttccttcagt
     1501 ttcagggtcc tggaagagat acagaaatac aaaaaatcgg ggctgacctt tgctccggaa
     1561 gccggtaccc agagactgag ggacattatc aacaagaata taaccgatga agacatattc
     1621 tcttccgtga gaaaagctct ggaactgggt tggcagcaga taaagctgta tttcatggtg
     1681 ggtcttcccg gggaaacgga agaagatcta cagggaatag ctgatatagt aaaaaaaata
     1741 ctggatatca attatgaggt gaacggtcgc aaaggcgggc gtttcaacgt tacagtcagt
     1801 gtttcgaatt tcgttccaaa agcccacaca ccttttcagt gggaagccca ggacagtgaa
     1861 gaaagtttcc ttgaaaaaca ggagtatatt cgtcggagac tgcccaaaaa gaatgttacc
     1921 tataattacc acggagcata caaaagtatc ctggaagggg tctttgccag gggagatgca
     1981 gaatgcggca gggtcctttt ggaagcatgg aaaaacggtt gcaggttcga cagctggaac
     2041 gaacattttg accggcaaaa atggagagaa gcttttgaaa aaacaggtat ctctgcagac
     2101 aaatatacag tgaggaagag aagttatgac cagagcctgc catgggactt tatcgatact
     2161 tatataagcc gggaattcct tataaatgag gccaaaaagg ctcaaattgg ccaaaccaca
     2221 ggggactgca gggaaggatg cgaagactgc ggcatcagca gaagtgcagg ctgtccgtta
     2281 gcaagaggaa agaaaaaagg aacaacggaa taaaagcaga cagagccaag gaaaaaaaag
     2341 aagacagagc caagggaaaa aacaagacag agccaaggga aaaaagaaga caaagccaag
     2401 aaaaaaagaa gacaaagcta atggtataaa ataccgcaaa acgggagaat aaatttgcca
     2461 agatataaac tgacacttac aaaggaagga tatatacgtt atacttcgca tcttgatatg
     2521 ttgag
//
LOCUS       DAONHK010000152         2487 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_35765_length_2487_cov_3.486842, whole genome shotgun sequence.
ACCESSION   DAONHK010000152 DAONHK010000000
VERSION     DAONHK010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35765_length_2487_cov_3.486842"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..905)
                     /locus_tag="PLM92_05405"
     CDS             complement(<1..905)
                     /locus_tag="PLM92_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004626220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HPR24608.1"
                     /translation="MAPPYEYHGYAMDTEITIKIMDRDTHRIPDGAKDEIERLEKKLS
                     RFIENSEISRINMSAGKEAVTVSTETMNILSEAKRFSRISLGVFDITAAPLIDLWRNS
                     RIDGPPDKKKISRALALVDHRDLVLDKKEKKAFLKNAGQMIDLGGIGKGYAGDCCIKK
                     LKEGGISSAFLNIGGNVCLLGKRKDGTPFRVGIRHPRKKDTLLGAVEVTEASGKAVVT
                     SGDYERCFIDKEGKRVHHIIDISKGMPATSGLVSVSVIHESGMIADALSTAVFAAGIQ
                     KGKGFIEKIKGAEAILMDEDQSIFVT"
     gene            complement(906..>2487)
                     /locus_tag="PLM92_05410"
     CDS             complement(906..>2487)
                     /locus_tag="PLM92_05410"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HPR24609.1"
                     /translation="DNKELSFYDLIAAEYRELLWQQWETILSEKKTFNYEYEIVKASG
                     ERRWVLEMGQGVYDEQGDVEALEGIIIDITDHKVMENTLKYNNDHDRWTGLHNRTYLE
                     KMIDEDTKKEFAGKRALISINLFTLKSLTKIYGFHYTQDLVKEIAKALKVHCDNEKRL
                     FYTYADRFVLYVKNYNEKEYLELLCEKIALTLDSILSIERVGGGIGVLEISKDFEGNA
                     DVILKNLLLASEKATEIKDKDFGICFYDSKMQKQILREQEIKWELSRLASFGNKGNLY
                     LQYQPIVDTESKAICGFEALARFKNENLGQVPPLEFIPVAEKTKLIIPIGELVIRQAF
                     SFLKRLNEEGYEDINVSVNISVIQILKEDFCSNFFRLMDEMKVRPENVGIEITESVFT
                     SSYIEINKILGEMKKAGIHIYIDDFGTGYSSLARERELNINSLKIDKFFVDDLMVLDP
                     QREIIIDIISMAHKFDHKAIAEGVEHEKQRKNLIKCGCDRIQGFLISKPLDEDDAIKF
                     LRKNTKRKKDSTKKGEQM"
BASE COUNT          575 a          573 c          440 g          899 t
ORIGIN      
        1 gtgacaaata tactctgatc ttcgtccatc agaattgctt cggctccctt gatcttctct
       61 ataaatcctt ttcccttttg gatacctgct gcgaaaacag ctgtagacag tgcatcggct
      121 atcatgccgc tttcatggat cacggaaacg cttacaagcc ctgaggtggc cggcattccc
      181 tttgatatgt cgattatatg atgcaccctt tttccttctt tgtcaataaa gcagcgttca
      241 taatcgccgg atgtcaccac agctttgccc gaagcttccg ttacttccac tgctcccaga
      301 agggtgtctt tttttctcgg atgtcttatt ccgactctga agggagttcc gtcctttctt
      361 tttccaagga gacagacatt gcctcctata ttcaaaaatg cggaagaaat gccgccttct
      421 tttagttttt ttatgcagca gtctccggca tatcctttgc ctatgcctcc gaggtctatc
      481 atttgaccgg catttttaag aaatgctttt ttctcttttt tatcaaggac aagatctctg
      541 tgatccacca gggcaagtgc acgactgatc ttttttttat caggaggtcc atcaatcctg
      601 ctgttcctcc aaagatcaat tagtggtgca gcggttatgt caaatacacc aagggatatc
      661 cgggaaaatc tttttgcttc cgaaaggata ttcatcgtct ctgtgcttac tgtaaccgct
      721 tctttgccgg cagacatgtt gattctgctg atttcactgt tttcaataaa acgactcagc
      781 tttttttcca gtctttctat ttcatctttt gcgccatccg ggatcctatg ggtatccctg
      841 tccattattt tgatggtgat ttctgtgtcc atggcatatc catggtattc ataaggcggg
      901 gccacttaca tttgttctcc tttttttgta ctgtcctttt tcctttttgt gtttttcctg
      961 aggaatttta ttgcgtcatc ttcatccaga ggtttactta taaggaatcc ctgtattctg
     1021 tcgcaaccgc attttatgag atttttcctt tgcttttcgt gttctactcc ttcggctatt
     1081 gccttatgat cgaatttatg agccatggaa atgatgtcga ttattatctc tctttgtggg
     1141 tcaagcacca tcaggtcatc tacgaagaat ttgtctattt tcagggaatt tatattcagt
     1201 tctctttctc tggccagaga tgaatatccg gttccgaaat catcgatata aatatggatg
     1261 cctgcctttt tcatttctcc caggatcttg tttatttcga tatagcttga agtaaatacg
     1321 gactctgtta tttctattcc cacattctcc gggcgaactt tcatttcatc catcagccgg
     1381 aaaaaattac tgcaaaaatc ctccttgaga atttgtatga cggaaatatt tactgaaaca
     1441 tttatatctt catatccttc ctcatttaat cttttcagaa aactaaaagc ctgtcttatt
     1501 acaagttctc ctatgggtat tatcaatttt gtcttttctg ctacaggaat gaattccaaa
     1561 ggcggcacct gtcccaggtt ctcatttttg aacctggcca gtgcttcaaa tccgcatatc
     1621 gcttttgatt cggtatccac tatgggctga tactgcagat ataagtttcc tttgttgccg
     1681 aatgaggcca gtctgctaag ctcccacttt atctcctgtt cccttagtat ctgtttctgc
     1741 attttggaat cataaaagca tatcccgaaa tctttgtcct ttatttcagt tgctttttcg
     1801 gaagccagaa gaagattttt cagaataaca tccgcattgc cttcaaagtc tttgctgatt
     1861 tccaggacac ctatgcctcc tcctaccctt tctattgaca gtatcgaatc cagggtaagg
     1921 gctatttttt cacagagcag ctccagatat tctttttcgt tatagttttt tacgtacaaa
     1981 acaaaacggt ccgcatatgt ataaaaaaga cgtttctcat tatcacaatg aactttaagt
     2041 gcttttgcta tttccttgac cagatcctgt gtgtaatgga atccgtatat cttggtcagc
     2101 gacttaagag taaaaagatt gatgcttatc aaggcccttt tgccggcaaa ttcttttttc
     2161 gtatcctcat cgatcatttt ttcaagatag gtccggttgt gaaggccggt ccatcgatcg
     2221 tggtcattat tatattttag cgtattttcc attaccttat gatctgttat atctattatt
     2281 attccttcta aagcttccac atcgccctgt tcatcataaa cgccctggcc catttcaagg
     2341 acccatctgc gctccccgga ggctttgact atttcatatt cgtaattgaa ggttttcttt
     2401 tcagaaagta ttgtctccca ctgctgccag agcagttctc tgtattctgc agcgatcaga
     2461 tcataaaagg aaagttcctt gttatct
//
LOCUS       DAONHK010000153         2484 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_35827_length_2484_cov_3.146562, whole genome shotgun sequence.
ACCESSION   DAONHK010000153 DAONHK010000000
VERSION     DAONHK010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35827_length_2484_cov_3.146562"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            75..632
                     /locus_tag="PLM92_05415"
                     /pseudo
     CDS             75..632
                     /locus_tag="PLM92_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002605121.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
     gene            complement(744..1622)
                     /locus_tag="PLM92_05420"
     CDS             complement(744..1622)
                     /locus_tag="PLM92_05420"
                     /inference="COORDINATES: protein motif:HMM:NF014376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HPR24610.1"
                     /translation="MKFRQPVIFELNSGTNALVSEAEAVPFHMHDSIVEIVCVEKGRL
                     KISDCALDHILGPGDVYIFNAMDAHRMHPLSPGTLILTVHIDKRYYSRFFRDLRDIYF
                     ICGSESPEKDSRELRHLKHLMHALLKECSCAAPSDLVTENLVREMLGLLCEQFRYYFY
                     KKDPDGIYMLFRRATSTAAMHHNKRAYEIVDYIYENFSRRLRLTDVASKYYLSPSHMS
                     RYIKKVIGISFSEILALARCEEAERLLFDTEKTVDEIAGEVGFVNRKHMAVNFRKWYG
                     KTPTRWRKSLEEEKNF"
     gene            1911..>2484
                     /locus_tag="PLM92_05425"
     CDS             1911..>2484
                     /locus_tag="PLM92_05425"
                     /inference="COORDINATES: protein motif:HMM:NF018012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HPR24611.1"
                     /translation="MFNGKEKKMLMQSKVLSDEEINVIHKKSIRILEETGVKFPSEKA
                     LDLLEGAGARIDREKELAYINEDMVNKALKTSPKEFTLGAPDPKYDLKLPSALPTHNI
                     DGCGMYVYDHDSRKKRLGTVKDVEDSFRIFQTMDMGSIGWAPIAAADLPPKVAGVMET
                     GLFYINSSKHYQDEETSMKGVGYHMEMAKAI"
BASE COUNT          870 a          502 c          565 g          547 t
ORIGIN      
        1 cggtggagac catagtaaag gccaatgagg gtgaagaccc ggtcatggtg gtgtaaactg
       61 ttgaagaagt agtaaatgaa aaagcgatat cctatgatac ggaagtcaaa aaatcaagca
      121 agtatgaaga gggaacggta aaggttgctg aagaaggaga gaagggcata aaagaagtta
      181 cttccatcgt gaaaaaagaa aacggagaag taacagacag caaagtcatc ggagagaaag
      241 taacaaaaaa acccgagtca aaagtcgtaa tcaaaggaac caaaatcagc aagaccggac
      301 aaaaagttgc aagctacgca ttgcagtttg ttggaaatcc ctatgtatgg ggaggaacaa
      361 gtcttaccaa cggagctgac tgttccggat tcactctaag cgtttattca catttcggca
      421 tttcactgcc tcacagtgca gccgctcaga gaaactacgg aaaggctgtt tcatacggcg
      481 atgcaagacc cggagatctg ttcttttatc cgcaccatgt tgcgatatac atcggtggcg
      541 ggcaaatcgt gcatgccagt acttccagta caggaataat agtaggaaat gccagctaca
      601 gacctctctc gtgtatcaga cgtcttttgt aaaaaagagc aaaaacaaaa aaggcttgga
      661 aaaacccaaa agtcaaacaa aaaaccaaaa caaaaatgag ctgcccatag tgcaaaaaat
      721 tgccgggcag cttttttatt gtgctaaaag tttttttctt cttccaagct tttgcgccac
      781 ctggtgggag tttttccgta ccattttctg aaattcaccg ccatatgttt tctgttgaca
      841 aagcccactt ctcccgctat ttcatccacg gttttttccg tatcgaaaag aagccgctcg
      901 gcttcctcgc atcgggcgag agccagtatc tcagaaaaag agatgccgat cacttttttt
      961 atatacctgc tcatatgaga gggactgagg taatattttg atgccacatc agtcagtctc
     1021 aggcggcggg aaaagttctc gtagatatag tcaactattt cataagctct tttattatgg
     1081 tgcatcgctg ccgtagaggt ggctctgcga aaaagcatat aaatgccgtc gggatctttt
     1141 ttgtaaaagt aataccgaaa ctgttcgcaa agaagtccca gcatttctct taccaggttt
     1201 tctgttacaa gatcactggg tgccgcacag gagcactctt ttaaaagagc gtgcataagg
     1261 tgtttcaggt gacggagttc gcgggaatct ttttcgggac tttcagatcc gcatatgaag
     1321 taaatatcac ggagatcccg gaaaaatctg ctgtaataac gcttgtcgat atgaactgtc
     1381 aaaatcaggg tgccgggcga taaaggatgc atcctgtgag cgtccattgc attaaaaatg
     1441 tagacatccc ctggaccaag gatatgatca agagcacagt cggagatctt gagacggcct
     1501 ttttcgacac agactatttc tacgatgctg tcatgcatgt ggaaaggaac tgcttccgcc
     1561 tctgagacaa gggcatttgt gcccgaattc aattcaaaaa ttacaggctg acgaaatttc
     1621 ataaatcaat gatatcaaaa atataccctg ataacaacaa aaatataccc ctattttcag
     1681 gcggaaagac atattattta atcatagaga tactgtaaat gaaaacaaag aaacaaagaa
     1741 gcaaatagaa accaaaaaac aaagaaacca aaaaacaaag gaactaaaaa acgaagaaaa
     1801 cccaagaacc caagaaaacc aggaaacgaa gcaaagaaga aataaaagaa acacaaggaa
     1861 agcgaagaca ggaaaagaaa agtcaggtaa atcacaacag acagaaacta attttcaacg
     1921 gaaaggagaa aaaaatgctg atgcagtcaa aagtactcag cgatgaggaa atcaatgtca
     1981 tacacaaaaa aagcataagg atacttgaag aaaccggagt gaaattcccc agcgaaaaag
     2041 ccctggatct gctggaggga gcaggagccc ggatagacag agaaaaagag ctagcatata
     2101 taaatgaaga catggtgaat aaggccctaa aaacatcacc caaagagttc actctcggag
     2161 caccggaccc caaatacgat cttaagctgc cttctgccct tccgacccac aatatcgacg
     2221 gttgcggcat gtatgtctat gatcacgaca gcaggaaaaa aagactgggg acggtaaaag
     2281 atgtagaaga cagtttcagg atattccaga ccatggatat gggcagtata ggatgggcgc
     2341 ccatcgctgc ggctgatctg cctccgaagg ttgcgggcgt aatggaaaca ggactttttt
     2401 atatcaacag cagcaagcat tatcaggatg aggaaaccag catgaagggc gtaggctatc
     2461 atatggaaat ggcaaaggcc attc
//
LOCUS       DAONHK010000154         2482 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_35860_length_2482_cov_3.751957, whole genome shotgun sequence.
ACCESSION   DAONHK010000154 DAONHK010000000
VERSION     DAONHK010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2482
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35860_length_2482_cov_3.751957"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..723)
                     /locus_tag="PLM92_05430"
     CDS             complement(<1..723)
                     /locus_tag="PLM92_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HPR24612.1"
                     /translation="MKKKIMLGNEAFARGAAEAGTKVISSYPGTPSTEITENAAKYDS
                     IHVEWAPNEKVGAEVVIGAGIAGARAMTCMKHVGLNVAADPLFTAAYTGVNAGIVFVV
                     ADDNGCYSSQNEQDSRFYARSAGVPMLEPADSKECLEYVKLAFELSEKYDTVMMIRSN
                     TRISHSRGVVELGEIPVPKLKEYKKNTGKYVAMPAAAKKLHIAQEKRIQKFAEDSSRS
                     RINRIEEGSKEMGIITSGICYQY"
     gene            complement(726..2024)
                     /locus_tag="PLM92_05435"
     CDS             complement(726..2024)
                     /locus_tag="PLM92_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004617924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HPR24613.1"
                     /translation="MIWNKKIECAGRDEIKEIQLVRLKQTVERCYERVPFYRKKFDET
                     GLKPKHIRTLEDVRLLPFTTADDLKNNYPFGLFAVDKKEIVRVHGSSGTTGKPKIEGY
                     TRNDLENWTEILARVISAAGVTSEDITQISFGYGLFTGGFGLHYGMERVGAMVVPLSS
                     GNTERQLMIMQDFGVTVLVSTPSYALYMADEIEKRGIDKSKIKLRLALFGGEGHTEEM
                     RAKIEKQLMITATENYGLSELGGPGYSGECYLQKGMHIAEDHYFSEIINPDKLNTLDM
                     GEKGELVITSLTREAFPMLRYRTRDITWLDDTPCPCGRTSMKMAKVQGRSDDMLIIRG
                     VNVYPSQIESVIMSTKEIQPFYEIVVYNEGYMDRIEVRVEFSDASLLDNFKKLEELRG
                     RIGHDLRTVLNIEAKVTLVSAGTLPRFEGKGKRVIDKRNR"
     gene            2259..>2482
                     /locus_tag="PLM92_05440"
     CDS             2259..>2482
                     /locus_tag="PLM92_05440"
                     /inference="COORDINATES: protein motif:HMM:NF013986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HPR24614.1"
                     /translation="MDEMAQTGTKWKENITISSMCGIFAESEVVSLIAENKNTADIIH
                     GLNKSVASKTVSLLSRIPVREKIMITGGVA"
BASE COUNT          605 a          601 c          453 g          823 t
ORIGIN      
        1 atactgatag catatcccgg aggttattat gcccatttct ttactgccct cttctatcct
       61 gtttatccgg gacctgctgc tgtcttcggc aaatttctgt attctttttt cctgggctat
      121 gtgaagtttt ttggccgccg caggcatggc tacatatttg cctgtatttt ttttatattc
      181 tttaagtttg gggacgggga tctctccaag ttcaactact ccccttgaat gagatatcct
      241 ggtgtttgat cttatcatca ttactgtatc gtatttttca ctcagttcaa aagccagttt
      301 cacatattca aggcactcct tcgagtctgc cggctccagc atcggtactc ccgcacttct
      361 ggcataaaac ctggaatcct gctcgttctg gcttgaatag catccgttat catctgcaac
      421 aacaaaaact attccggcat tgactcccgt ataggccgct gtgaaaagag gatccgctgc
      481 cacattaagt ccaacatgct tcatgcatgt catcgctctt gctccggcta tgcctgcccc
      541 gataacgact tcagcaccaa ctttctcgtt gggtgcccat tccacatgta tactgtcgta
      601 ttttgctgca ttttctgtta tttctgttga aggtgtcccg ggatatgatg aaatcacttt
      661 tgtcccggct tctgctgcac ccctggcgaa ggcttcattg cccagcatta tctttttttt
      721 catagttatc tgttcctctt gtctattact ctttttcctt ttccctcgaa tctgggcagt
      781 gttcccgcag acaccagagt tacttttgcc tctatattca gtaccgttct cagatcatga
      841 cctatccttc ctctcagttc ttcaagcttt ttgaagttat caagcaatga tgcgtccgag
      901 aattccactc ttacttctat cctgtccata taaccttcat tgtagaccac tatctcatag
      961 aaaggctgta tctcttttgt ggacattatt acggattcaa tctgggaagg atatacattc
     1021 acgcccctta ttatcagcat atcatcggag cgtccctgaa cttttgccat tttcatggaa
     1081 gtcctgccac aggggcaggg agtatcatca agccatgtta tatctcttgt cctgtatctc
     1141 agcatgggga aggcttccct tgtaagtgat gttattacca gttcaccttt ttctcccata
     1201 tcaagagtat tgagtttgtc cggatttatt atctcagaaa aataatggtc ttctgcaata
     1261 tgcattccct tttgcagata acattctccc gaatatcccg ggcctcccag ctcggacagt
     1321 ccgtagtttt ctgtggctgt tatcatcaac tgtttttcaa tttttgccct catctcttcc
     1381 gtatgtcctt ctccgccgaa gagagccagt cgaagtttta ttttgctttt atctatacct
     1441 cttttttcga tttcatccgc catgtaaagt gcataagaag gcgttgacac cagtactgtg
     1501 actccgaaat cctgcattat cataagctgt ctttctgtat ttccgcttga aagcggcacc
     1561 accatggccc ctactctctc catgccgtag tggagcccga aacccccggt aaaaagtccg
     1621 tatccgaaag atatctgagt tatgtcctcc gatgtgactc ctgcagcgga tattactctg
     1681 gccagtatct ctgtccagtt ttcaagatcg tttcttgtat atccttctat ctttggcttc
     1741 cccgtggttc ccgaagaccc gtgcaccctt actatctctt ttttatcaac agcaaaaagt
     1801 ccgaaaggat aattgttctt gaggtcatcc gccgttgtaa agggcagaag tctaacatcc
     1861 tccagtgttc ttatgtgttt gggcttaagt cccgtttcat caaatttctt tctgtaaaag
     1921 ggtactcttt cataacatct ttctacggtt tgcttgagtc gtaccagctg tatctctttt
     1981 atctcatctc taccggcgca ttcaattttt ttgttccaga taatgtgttc cattttttct
     2041 tcccttttct taaataatac acttattgta tagtaaagaa ggtgtaaaat caaacacttc
     2101 tgcccattct atgctataat atgccattat gaacaaagta atatcgaaat tcggaaaaac
     2161 agtagaaaaa caaataaata aaaatccaaa agcagcctac tcagtgtgcc atagaaagct
     2221 atttccttga gacaatggca aaagtcctgc agatgggaat ggacgaaatg gcgcaaacgg
     2281 gaacgaaatg gaaagaaaat ataactattt caagtatgtg cggcatattt gcagaatccg
     2341 aagttgtttc tcttattgcg gaaaataaaa ataccgcaga tatcatacat ggactgaaca
     2401 aatccgtggc atcaaaaaca gtttctcttt taagccgtat accggtgcgc gaaaaaatca
     2461 tgataacagg aggtgttgca aa
//
LOCUS       DAONHK010000155         2340 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_39242_length_2340_cov_3.485339, whole genome shotgun sequence.
ACCESSION   DAONHK010000155 DAONHK010000000
VERSION     DAONHK010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39242_length_2340_cov_3.485339"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            259..573
                     /locus_tag="PLM92_05445"
     CDS             259..573
                     /locus_tag="PLM92_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPR24615.1"
                     /translation="MVEQVFRLYKGNEKTIEKVLLDENINYMHMVLNKTEGLPEHFSN
                     SNLYMTVLRGRLSIGLDDQEIHEYEAGDLLKIPFKTKMNVKNLHDDTLELIVVKAPAP
                     KD"
     gene            653..817
                     /locus_tag="PLM92_05450"
     CDS             653..817
                     /locus_tag="PLM92_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004353445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HPR24616.1"
                     /translation="MKKYTCIPCGYVYDPELGDSDGGIAEGTPFEELPDDWHCPICFV
                     GKDEFEPTEE"
     gene            832..>2340
                     /gene="hcp"
                     /locus_tag="PLM92_05455"
                     /gene_synonym="priS"
     CDS             832..>2340
                     /gene="hcp"
                     /locus_tag="PLM92_05455"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HPR24617.1"
                     /translation="MSMFCYQCQETAGGQGCKIRGVCGKTEEVAKLQDLLIYTLKGIS
                     DIVTKSKTDAVSIEKVNHEVLNSLFMTITNANFDDGSIETQIKKMIALRDELQKPAEP
                     DCLHDAALFNVKSRESMLEKADSIGVLATENEDVRSLREMITYGLKGMAAYTEHANNI
                     GKENLEINAFIYEALAATLDDSMTADDLVVLTMKTGEHGVKAMALLDEANTTRFGTPE
                     ITEVNIGVKNNPAILITGHDLTDLEQLLEQTKNTGVDVYTHSEMLPSHYYPAFKKYEN
                     FVGNYGNAWWKQIDEFGPFHGPILFTTNCIVPPRSEEIKGRIFTTGSTGYPGCKHILP
                     DENGKKDFSEIIELAKTLPAPEGIESGSIVGGFAHDQVIALADKVVDAVKSGAIKKFF
                     VMAGCDGRMKSREYYTDFAKKLPGDTVILTAGCAKYRYNKLGLGDIDGIPRVLDAGQC
                     NDSYSLAVIAMKLQEVFGLDDINKLPIAFNIAWYEQKAVIVLLALLYLGVKNI"
BASE COUNT          797 a          454 c          496 g          593 t
ORIGIN      
        1 gaaacatgtt cttctttttt ctttatcaat ttacaccatt atatgggcac gacctgaaat
       61 aagaaagtat tgtttgaaaa atacagaatc agtgtaaatc ataaaggtaa attgattata
      121 taagcaaatg taaaaaatat atcttactgc tttataatta tatcataaaa aacgttgtta
      181 tgttgtaaat tcaacataca aacctgattc tttatagtat aatacaattt aaattatacc
      241 agatgggagt gataagccgt ggtagaacaa gtattcagac tatataaggg aaacgaaaaa
      301 acaatagaaa aagtactgct tgacgagaat ataaactaca tgcacatggt actaaacaaa
      361 actgaagggc tacccgaaca tttctcaaat tcaaaccttt atatgactgt gttgcggggc
      421 agactttcta tcggtcttga tgatcaggaa atacatgaat atgaagcagg agatcttctc
      481 aagataccct tcaagacaaa aatgaatgtc aaaaaccttc acgatgacac actggaactt
      541 attgtcgtaa aagcccctgc acccaaggat tgatgatcct ttgaggaatg gaccaaaata
      601 taaaaaagaa tatcttttat cagaaaaacg ttttacagga aaggagaaac aaatgaagaa
      661 gtacacatgt atcccctgcg gatatgtata tgacccggaa ctgggagatt ccgacggagg
      721 tatcgctgaa ggaacccctt ttgaggagct tcccgatgac tggcactgtc caatatgttt
      781 tgttggcaaa gacgaatttg aacctacaga agaataaagg aggaaaacag tatgagtatg
      841 ttttgttacc aatgccagga aacagccgga ggacaaggct gtaagatccg tggtgtatgc
      901 gggaagacgg aagaagttgc aaagcttcag gatcttctga tttacaccct taagggcatt
      961 tcagacatcg ttacaaaaag taaaacagat gcagtgtcca ttgaaaaagt caaccatgaa
     1021 gttcttaaca gtctttttat gactattaca aatgcaaact ttgatgacgg ttcaattgaa
     1081 actcaaataa aaaaaatgat agccttaagg gacgaactcc aaaagcctgc cgagccggac
     1141 tgccttcatg atgctgcgct gttcaatgta aaatcaagag agtccatgct cgaaaaggcc
     1201 gattccatcg gtgtactggc cactgaaaat gaagatgttc gttctcttcg tgaaatgatc
     1261 acatacggac ttaagggaat ggccgcatat accgaacatg ccaacaatat tggcaaagaa
     1321 aatcttgaaa taaatgcttt tatctatgaa gctcttgctg ccacactgga tgactccatg
     1381 actgcagatg acttggtagt tcttaccatg aaaacaggag aacacggtgt aaaagccatg
     1441 gctcttctgg atgaggccaa taccacgaga ttcgggactc ccgagatcac agaggtaaat
     1501 ataggtgtaa aaaataatcc tgcgattctt ataacaggcc atgatcttac agatctggaa
     1561 cagctgctgg aacagacaaa aaacacagga gtagatgttt atacccacag cgaaatgttg
     1621 ccttcacact actacccggc tttcaaaaag tacgaaaatt ttgtgggtaa ttacgggaat
     1681 gcctggtgga aacagataga tgaattcgga cctttccacg gaccgatcct ttttaccacc
     1741 aactgtatag tccctccccg aagcgaggaa ataaagggaa ggatatttac cacaggctcc
     1801 accggctatc ccggatgcaa gcacatactc cctgatgaaa acgggaaaaa agacttttct
     1861 gagataatag agctggcaaa aactcttccg gctcccgagg gaatcgaatc aggcagtatc
     1921 gtgggcggtt tcgcacacga tcaggtaata gctctggccg ataaggttgt tgatgcggtc
     1981 aaatccggtg caataaaaaa attctttgtt atggcaggtt gcgacggcag aatgaaatca
     2041 agagaatact atacagattt cgcaaaaaaa cttcccgggg atacagtaat actgaccgcg
     2101 ggatgtgcaa aataccgtta caataaactg ggccttggag atattgacgg cataccaaga
     2161 gtacttgatg caggccagtg taatgactcc tattctctgg ccgtgatcgc catgaaacta
     2221 caggaagtat tcggtcttga cgatattaac aagctgccca tagcattcaa tatagcctgg
     2281 tatgaacaga aagctgtgat cgttcttctt gccctgcttt atctcggtgt taagaatata
//
LOCUS       DAONHK010000156         2314 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_39920_length_2314_cov_4.363435, whole genome shotgun sequence.
ACCESSION   DAONHK010000156 DAONHK010000000
VERSION     DAONHK010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2314)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39920_length_2314_cov_4.363435"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="PLM92_05460"
     CDS             <1..690
                     /locus_tag="PLM92_05460"
                     /inference="COORDINATES: protein motif:HMM:NF025925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HPR24618.1"
                     /translation="ACSTAAGPAFEGASINHGMRAVPGAVEKIMIKKEKVLIKTIDGI
                     QPKGICGSGIIDAVAQMLDAGVVNRTGRILSAEELKKNGMPGAAMRINEKESREFSLV
                     IREHDEDIVLTQKDIREVQLAKGAISAGINIMLEKAGRKKEEIKKIVLAGAFGNSINK
                     ESAVAIGLLPDIDIKKIHAVGNAAGAGAIMVLADDREQKKALELPRITQHVELAEIKG
                     FQEKFISAMSF"
     gene            784..2133
                     /locus_tag="PLM92_05465"
     CDS             784..2133
                     /locus_tag="PLM92_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019123680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HPR24619.1"
                     /translation="MIDRQKLKSQMKKEIEMFEKNHKKSGELYRKAKESLLQGVPMNW
                     MTKWAGSYPVYVANAKGAHFQDVDGNDYLDLCLGDTGSMIGHAPEPAVRAITEYAKKG
                     TTFMLPTEDAAWNGEELNRRFGMKYWQFATSATDSNRFALRLAREITGRKKIVVYNWC
                     YHGSVDETVAVIDEKTGKTVVKPGSIGPQCDPELTTKVIEWNDVPALEAALMDEDVAA
                     VLAEPVMTNCGIVHPKPGYHDALRKLAKKYGTLLIIDETHTICAGVGGCTKEYGLKPD
                     MVVVGKTVAAGIPCAAYGFTKEVGDKAAAAIPRELCDIGGIGGTLAANALSMHVMRAV
                     LGEVLTQEYYDKNIPLAKRFNEGVQSVISKYGLPWNTTQLGCRTEYWFRKEPAKNGGE
                     AEAAVDFELDQYMHLASMNRGFLMTPFHNMALISAATSEEDIDRHTMVFDEIVSNII"
BASE COUNT          803 a          424 c          596 g          491 t
ORIGIN      
        1 gcctgttcga cggcagcagg tccggcattt gagggagcct cgataaatca cgggatgcgg
       61 gccgtaccgg gggccgttga aaaaatcatg ataaaaaaag aaaaagttct gataaaaaca
      121 atagatggca tacagccaaa aggcatatgc gggtcgggca taatagatgc tgtggcacag
      181 atgcttgatg ccggagtagt gaacaggacc ggcaggatac tttctgctga agagctgaaa
      241 aagaacggta tgcccggagc ggccatgcga atcaacgaaa aagaaagcag agaattctca
      301 ctcgtgataa gagagcatga cgaagacata gttcttactc aaaaagatat aagagaagta
      361 cagctggcaa agggagccat atccgcggga ataaacatta tgcttgaaaa agcaggaagg
      421 aagaaagaag aaataaaaaa aatagtactt gcgggagcct ttggaaactc cataaacaaa
      481 gaaagcgcag tggccatagg gctgctaccg gacatagaca taaaaaagat ccatgcggta
      541 gggaatgccg ccggagccgg agccataatg gtgcttgccg atgaccgtga acagaaaaaa
      601 gcattagagt tgcctcgaat aacacagcat gttgaactgg cggagatcaa aggctttcag
      661 gaaaaattta tttcagccat gtctttttga tcaaaacatt tttcagaaaa acaaacaccc
      721 attgataaaa acagcaagaa taagatataa taaaattcaa atataaataa acaggaggta
      781 attatgatag acagacaaaa gctaaaaagc cagatgaaaa aagaaatcga aatgttcgaa
      841 aaaaatcaca aaaaatcggg tgagctttac agaaaagcca aagaaagtct gctgcaggga
      901 gtgccaatga actggatgac aaagtgggcg ggcagctatc ctgtttacgt ggctaatgca
      961 aaaggagccc attttcagga tgttgacggc aatgattatc tggatctatg cctgggagat
     1021 accggctcta tgatcggcca tgcacctgag ccggcggtaa gagccataac tgagtatgca
     1081 aaaaagggca ccacttttat gctgccgaca gaagatgccg cttggaacgg cgaagaactt
     1141 aacagacgat tcgggatgaa atactggcag tttgccacaa gtgctacaga ctcaaacaga
     1201 tttgctctca gactggcaag agagataacc ggtagaaaaa agatagttgt ctataactgg
     1261 tgttatcacg gttctgtgga tgaaacagta gctgtgatcg atgaaaaaac aggaaagaca
     1321 gtggtaaaac ccggaagtat aggtcctcag tgtgatccgg aactgacaac aaaggtgata
     1381 gaatggaacg atgttcctgc ccttgaagcg gcacttatgg acgaagatgt ggctgcagtt
     1441 ctggcggaac ccgtaatgac caactgcggg atcgtgcatc ctaaacccgg ataccatgat
     1501 gcactcagga aactggcaaa aaaatacggc acattactta ttatagacga aacacatacc
     1561 atttgcgccg gtgtgggcgg atgcacaaag gaatacggac tgaaaccgga tatggtggta
     1621 gttggcaaga ccgttgccgc aggcataccc tgtgctgcct atggatttac caaagaagtg
     1681 ggtgataaag cagctgcagc catacctcgt gaactttgcg atataggagg aataggaggg
     1741 acactggcag ccaatgccct gtccatgcac gtgatgagag ctgtccttgg ggaagttctt
     1801 acacaggagt attatgacaa gaatatacct ttggcgaaga gattcaatga aggtgtgcag
     1861 agtgtaataa gcaagtatgg tcttccgtgg aatactacgc agctgggatg cagaactgaa
     1921 tactggttca gaaaagagcc tgcaaagaac ggaggagaag ctgaagctgc agtggatttc
     1981 gaattggatc agtacatgca tctggcatca atgaacagag gattccttat gactcccttt
     2041 cataacatgg cactgatatc agcggcaaca tcggaagaag atatagacag acataccatg
     2101 gtgtttgatg aaatagtatc aaatataata tagcagcaaa tgtgaaaaac tttaaatacg
     2161 gagcgatgaa atttttggaa aatgtcgctc ctttttattt tatcaaagat accggagggc
     2221 ttttgtctgc cggaaaaaag ttgtatactg gaaaaaagag ggtctgtaaa gtagatatga
     2281 aaaaaagagg ccatgagaat aatgtttctg ctaa
//
LOCUS       DAONHK010000157         2307 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_40125_length_2307_cov_2.736679, whole genome shotgun sequence.
ACCESSION   DAONHK010000157 DAONHK010000000
VERSION     DAONHK010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40125_length_2307_cov_2.736679"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /locus_tag="PLM92_05470"
     CDS             <1..513
                     /locus_tag="PLM92_05470"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HPR24620.1"
                     /translation="AHKENRKVHKENLNIQEESPYRERRMFISFSIIHRFAKIFLSAK
                     MEQLGTLKCSPPYIAAIYHNPGLSQNELSEFLKIDKGAVAKTVKCLLAKGFIEREKDP
                     LDKRGYKLYLTKKGETLMPNLLTIESGFERKITEGMSNEEKELLTKLINKAATNILSL
                     DTGEKNENNI"
     gene            497..>2307
                     /locus_tag="PLM92_05475"
     CDS             497..>2307
                     /locus_tag="PLM92_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006860027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPR24621.1"
                     /translation="MRIIFKYYKKYIPFILLIVGVLFGQAMCELALPGYMSDIINEGI
                     IMQNMDTIYRLGIIMIAVTLGSGICAVAGSFLASRTAAKSARDIRSSLFRKVTSFSDA
                     EMDQFSTASLITRSTNDVQMIQQASVMILRMAFYAPIMGVGALIKALNTNVSLTWTIG
                     LAVAVIFIIMLMAFFLVLPKFKVLQMKLDRLNLIMKERLSGLLVVRGFAREKYEQGRF
                     DEANIDLMKINVFVNKAMSLLMPALMLVMNLSGILIVWFGSKLIEANDMMIGDMLAFL
                     QYSMQIIMSFLFITMMFIMVPRAAVSAQRIGKVLAVEPSVKDAPAAGPQGKAGAEAVP
                     EKAGAEAVPGKAVSVSVSGKSGSTTTSNAPGSIKETQVSKGVLEFENVSFAYPDSKEK
                     TLCDINFTVSPGKTTAIIGGTGSGKTTLVRLITRFYDVTGGRILLDGNDIRNIPQKDL
                     RDVIGYVPQKGILFSGTIADNLRFGKEDASPEEMESAAETAQALDFIKSKNEGFEELI
                     TEGGTNVSGGQKQRLSIARALIKDPHIYIFDDTFSALDFKTDAALRKALKQKIEDASM
                     IIVAQRINTIMDADQIIVLDEGEIAGKGTHKELLSSC"
BASE COUNT          723 a          497 c          504 g          583 t
ORIGIN      
        1 gcccataagg aaaaccgtaa agttcataaa gaaaatctta atatacaaga agaaagtcct
       61 tacagggaaa ggcgcatgtt cataagcttt tcaataatac acagattcgc aaaaatattt
      121 ctctcagcaa aaatggaaca gctcggcacc ttgaagtgct cgccccctta tattgcggcg
      181 atctatcata atccgggact ttctcaaaat gaactgtcgg aatttcttaa aatagataaa
      241 ggcgccgtcg caaaaacagt caaatgtctt ttggccaaag gatttattga aagggaaaaa
      301 gaccctctgg acaaacgcgg atataaactg tatctcacca aaaaaggaga gacccttatg
      361 ccgaatctgc tgaccataga atccggattt gaacggaaaa taacagaggg tatgtccaat
      421 gaggaaaaag aacttttaac aaaactcata aataaagccg cgacaaatat tttgtccctc
      481 gatacaggag aaaaaaatga gaataatatt taagtattac aaaaaataca ttcccttcat
      541 acttttgatc gtaggtgtac ttttcgggca ggccatgtgc gaactggccc ttcccggata
      601 tatgtcagac ataataaatg aaggaataat aatgcagaac atggatacca tttacaggct
      661 gggcattatt atgatagccg taactctggg aagcgggata tgtgctgtcg caggaagctt
      721 tcttgcctcc cgcactgccg ccaaatcagc ccgtgatata agatcttctt tgttccgcaa
      781 agtcacttct ttttccgatg ctgagatgga tcagttttcc acagcgagcc tcataacccg
      841 ttccaccaac gatgttcaaa tgatacagca ggcttccgtt atgatcctgc gcatggcatt
      901 ttatgcgccg ataatgggag ttggcgccct gataaaagcc cttaacacca acgtatccct
      961 aacatggacc atcggtcttg ccgtggcagt tattttcatt ataatgctga tggctttctt
     1021 ccttgtactt cctaaattca aagtcctgca gatgaaactt gaccggttga atctgataat
     1081 gaaagaacgg ctttcgggat tgcttgtcgt caggggtttt gcaagagaaa aatatgaaca
     1141 gggacgcttt gatgaggcaa atatagatct tatgaaaata aatgtttttg taaacaaagc
     1201 catgtctctt cttatgccgg ctctcatgtt ggtaatgaat ctttccggaa tactgatcgt
     1261 gtggttcggt tcaaaactaa tagaggcaaa cgacatgatg atcggagaca tgctggcatt
     1321 ccttcagtat tcgatgcaga taataatgtc atttctcttt attaccatga tgttcatcat
     1381 ggttccaaga gctgcagttt ccgcacaaag gatcggcaag gttttggcgg ttgagccttc
     1441 tgtaaaagat gcgccggcag cagggccaca aggaaaagcc ggtgctgaag cagttccgga
     1501 aaaagccggt gctgaagcag ttccgggaaa agccgtttcg gtatcagttt cgggaaaatc
     1561 cggctcgacg acaacttcaa atgctcccgg aagcataaaa gaaacccaag tatccaaagg
     1621 tgttcttgag ttcgaaaatg tatcattcgc atatcccgat tcaaaagaaa agaccctttg
     1681 cgatatcaac ttcaccgtca gcccgggaaa aaccactgcc ataatcggtg gcaccggcag
     1741 cggcaaaacc actctggtaa gactgataac ccgcttttat gatgtgaccg gcggccgcat
     1801 ccttcttgac ggaaatgata taagaaatat ccctcagaaa gatcttcgtg atgtgatcgg
     1861 atatgtgccg caaaagggaa tactgttttc aggcaccatt gcggacaatc ttcgtttcgg
     1921 aaaagaagat gcttcgcccg aagaaatgga atcagccgca gaaacagctc aggctctcga
     1981 tttcataaaa agcaaaaatg aaggctttga agagcttata accgagggcg gcaccaatgt
     2041 ttccggaggt cagaaacaga ggctttccat cgccagggca cttataaaag acccccatat
     2101 atacattttt gacgatacat tttcggcgct tgattttaaa acagatgcgg ctcttagaaa
     2161 agcccttaaa cagaaaatcg aagatgcctc catgataata gtcgctcaga ggataaacac
     2221 cataatggat gcagatcaga tcatcgttct cgatgaagga gaaattgcag gaaaaggcac
     2281 ccacaaagag ttgctttcat cctgtga
//
LOCUS       DAONHK010000158         2271 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_41060_length_2271_cov_4.867780, whole genome shotgun sequence.
ACCESSION   DAONHK010000158 DAONHK010000000
VERSION     DAONHK010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41060_length_2271_cov_4.867780"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..648
                     /locus_tag="PLM92_05480"
     CDS             <1..648
                     /locus_tag="PLM92_05480"
                     /inference="COORDINATES: protein motif:HMM:NF014533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HPR24622.1"
                     /translation="TVVSGMAIGIDSYAHKGTLAKKGSTVAVLGCGTDICYPEVNGRL
                     KEQIEKNGLVVSEYPKGHPPSFYTFPRRNRIISGMSEAVVVISAGLNSGALITAELAA
                     DQGRDVYAVPGNINSIYNMGSNKLIRDGAIMISKIDDLLDDLSIKRKETEEVEAALGP
                     EEKTVFGLIAENGEVTADYLCRKTGKKSSYISGIVTVLEMKGVVETSSGKIFVAK"
     gene            709..>2271
                     /gene="topA"
                     /locus_tag="PLM92_05485"
     CDS             709..>2271
                     /gene="topA"
                     /locus_tag="PLM92_05485"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015311654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HPR24623.1"
                     /translation="MAAAKKHLVIVESPSKAKTIGKFLGSRYKVVASSGHLRDLPRSK
                     LGIDIEKDFEPEYISIRGRGSLIKELKKEAKKADRIYLATDPDREGEAISWHLQFLLG
                     IDNKEPCRIVFNEITKKAIKEAVKNPRPVDIKLVDAQQARRVLDRLVGYSISPLLWRK
                     IRRGLSAGRVQSAALSMICDRESEIKAFVPDEYWTVTALFKKQEVFSAKLQQFMGEKI
                     VLSKKEDADRVLSAIKGNSFTVESVEEKERQRKPYAPFTTSSLQQEASAKLNFTAKKT
                     MQIAQQLYEGIMIKGDGTAGLVTYIRTDSVRISEEAKEEAKTYINERFGTGYHSDNFF
                     SNKKKEVQDAHEAIRPSIIGLDPEQIKDSLNKDQYNLYRLIWSRFIASRMKPAVYSGV
                     SAVIENSGYMFKATGSKLKFDGFLKVYSPGKEDIKMLPHLEKGEKLLAEDILAEQNFT
                     QPPARFTEASLVKELEERNIGRPSTYAPIIATLTERKYIIREKKMLVPTDLGDMVNQM
                     MNEYFKNIVDLNF"
BASE COUNT          816 a          410 c          544 g          501 t
ORIGIN      
        1 actgtggtaa gcggaatggc tatcggcatc gattcgtacg cccataaggg aacccttgcc
       61 aaaaaaggtt ctactgtggc agtactggga tgcggtacag atatttgtta cccggaagta
      121 aacggcagat taaaagagca aatagaaaaa aacgggcttg tggtgtctga atatccaaag
      181 gggcatcccc cctctttcta tactttcccg agaagaaaca gaataataag cggtatgtca
      241 gaggcggtcg ttgttatttc cgccggtctt aacagcggag ctcttattac tgccgaactg
      301 gccgcggatc agggcaggga tgtatatgca gtgcccggga acataaacag catatataac
      361 atggggtcaa acaaactaat aagagacggg gcgataatga tatcaaaaat agatgacctt
      421 ctggatgacc ttagcataaa aagaaaagaa acagaagagg ttgaagcggc tctgggtcct
      481 gaagaaaaaa cagttttcgg actgattgcc gagaacggag aagtcactgc ggattacctt
      541 tgcagaaaga cgggaaagaa gtcttcctac ataagcggaa tagtgaccgt gctggaaatg
      601 aaaggagtgg ttgaaacttc atccgggaaa atttttgttg caaaataaca agaatgtaca
      661 tataatcatt ttttgtaaag tgtttggata attctaaagg gagtaaatat ggctgcagca
      721 aaaaaacatt tagttatagt tgaatcgcct tcaaaggcca aaaccatagg caaatttctc
      781 ggtagcagat ataaagtggt ggcatcctcg ggacatttaa gagatctgcc cagaagcaaa
      841 ctggggatag atatcgaaaa agattttgag ccggaataca tttcaataag gggaagagga
      901 tcacttataa aagaactcaa aaaagaagca aagaaggcag acaggatata tctggccact
      961 gacccggaca gagaaggaga agcgatatca tggcatctcc agtttctgct gggtatagat
     1021 aataaagagc cctgcagaat agtatttaat gagataacaa aaaaagcaat aaaggaagca
     1081 gtaaaaaatc ccagacctgt ggatataaaa ctcgttgatg cccagcaggc acgaagagta
     1141 ctggacagac tggtgggtta ttcaataagt ccacttttat ggagaaagat aagaagaggc
     1201 ctttctgcgg gaagagtcca gtccgcagcc ctttccatga tatgtgacag ggagagcgaa
     1261 atcaaagcct tcgtgccgga tgagtactgg accgtaacgg cccttttcaa gaaacaggaa
     1321 gtgttttcgg caaaacttca gcagttcatg ggagaaaaaa tagtcctgtc aaaaaaagaa
     1381 gatgctgaca gggtgctttc ggccataaaa gggaacagct tcacggtgga aagcgtagaa
     1441 gaaaaagaaa gacaaagaaa accttatgcg ccattcacaa ccagcagtct tcagcaggaa
     1501 gcatcggcaa aattaaactt tactgcaaaa aagaccatgc agatcgcaca gcagctttat
     1561 gagggaataa tgataaaagg tgacgggaca gcgggactgg tcacatatat aaggacagat
     1621 tcggtacgaa tatccgagga ggcaaaagaa gaggcaaaaa catatattaa tgaaagattc
     1681 ggaacgggat atcattcaga caactttttt tcaaacaaaa aaaaggaagt tcaggatgct
     1741 catgaagcca taaggccaag tatcataggg cttgatccgg aacaaatcaa agattcactc
     1801 aacaaggatc agtacaatct ttacagactc atatggagca gattcatcgc aagccggatg
     1861 aaaccggctg tttattcagg agtatctgcg gttatagaaa acagcggata tatgttcaag
     1921 gctacaggat ccaaacttaa attcgacgga tttctgaaag tttattctcc cggaaaggaa
     1981 gacataaaaa tgcttcctca cttagaaaaa ggtgagaaac tcctagcaga agatattttg
     2041 gcggaacaga atttcacaca gcctccggcg agatttacag aagcatctct tgttaaggag
     2101 ctggaagaaa gaaacatagg aagacccagt acctacgcac ccataatagc aacccttaca
     2161 gaaagaaaat atataataag agagaaaaag atgcttgttc ccactgacct gggagacatg
     2221 gtcaatcaga tgatgaatga gtatttcaaa aatatagttg atttgaattt t
//
LOCUS       DAONHK010000159         2267 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_41167_length_2267_cov_3.967450, whole genome shotgun sequence.
ACCESSION   DAONHK010000159 DAONHK010000000
VERSION     DAONHK010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41167_length_2267_cov_3.967450"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1048
                     /locus_tag="PLM92_05490"
     CDS             <1..1048
                     /locus_tag="PLM92_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPR24624.1"
                     /translation="DTEEYGFDTYKSIIGNDTESVARTEQELMGGLGGSRSDLSSREA
                     CFLLAEYMDFNKEHGIELPGDTREYIFMLEDAFSDPLSEEEKDVLANKTCTPIKSEYH
                     LINYFLMRCLGKDYKGAEYLCRGFDPWSVFPDIPMCTLCRNASDPSDLTDVRPGDTGG
                     DLSSEGVFLCQSLIEYDKKYSILVSRIEVHDMKVTDCRLESSMQISPAEAAMLLSRSE
                     FITVYEIMASVKDMEKYTWDLAAGTIVTIHDNGKLMMSFNENNDHVNKRVFRLSEDVF
                     GLYYITDYGQFIISSHSLKNIRKMEDVISSGPLDSLLLPLGKYEFKEPILYEFIQSDY
                     DNFEEFMEDLEPDE"
     gene            1060..2190
                     /locus_tag="PLM92_05495"
     CDS             1060..2190
                     /locus_tag="PLM92_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013841517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HPR24625.1"
                     /translation="MQKRTFKKTGEKISLLGMGVMRLPETSGGLIDEDLSVKIIRRAI
                     DGGVSYVDCAYTYHGGASEKVLGKALKNGYREKVFIADKIPSWLAKDEKDIEKMFEES
                     FARLDVDFIDMYLIHNITAPIWKKVQKLNVIPFLEQKQKEGKIGHLGFSFHDSLSLFK
                     EVVNFRRWDFCQIQLNYMDSDIQAGVEGLRYAASKGLSVIIMEPLKGGRLTDTVPSSI
                     KEIWDRAETKRSPAEWCFRWVADFPEVTTILSGMSTMEQLEENLKIIENCLPESLTEN
                     EHSLIEEVADKYRELIKYQCTDCKYCMPCPFKVDIPRCLSLYNEWFLYDKNPKTLMEY
                     KNWMDKRQRASNCADCKECESKCPQQLHITEAMKKIKNEFGE"
BASE COUNT          703 a          487 c          523 g          554 t
ORIGIN      
        1 tgatacggaa gaatacggtt ttgatacata caaaagcatt atcggaaatg acactgaaag
       61 cgtggcaagg accgaacagg aacttatggg aggtctcgga ggaagcagat ccgatctcag
      121 cagcagagag gcctgctttc tgctggcgga atatatggat ttcaataagg aacacggaat
      181 cgaacttccc ggcgatacaa gagaatatat atttatgctg gaagacgctt tttccgatcc
      241 cctgtcagaa gaagaaaaag atgtacttgc aaataagaca tgtactccca taaaatcaga
      301 ataccatctc atcaactact tccttatgag atgtctgggc aaagactaca aaggtgccga
      361 atatctttgc cgggggtttg atccgtggtc ggtttttccc gatatcccca tgtgtaccct
      421 atgcaggaat gccagtgacc cttcagacct caccgatgtc cggcccgggg ataccggggg
      481 cgatctttca tctgaaggag tgttcctctg tcagtccctg attgaatatg acaaaaaata
      541 ttccattctt gtcagccgca ttgaagtcca tgatatgaaa gtaaccgact gcagactgga
      601 gtcctccatg cagataagcc ctgcggaagc ggccatgctg ctttccagat cggaattcat
      661 aacggtatat gaaataatgg catcagtaaa ggatatggaa aaatatacct gggacctggc
      721 tgccggcacc atagtaacca tccatgacaa cggcaaactg atgatgtctt ttaacgaaaa
      781 caacgatcat gttaacaagc gcgtctttcg tctcagcgaa gatgtgttcg gtctttatta
      841 tataaccgac tacgggcagt tcattatttc ttctcattct ctgaagaata taaggaagat
      901 ggaggatgta atatcctcag gacctctgga ttcacttctt ctgccgttgg gaaaatatga
      961 attcaaagaa cccatccttt atgaatttat acaaagcgac tatgataact ttgaggaatt
     1021 tatggaagat ctcgaaccgg acgaatagga ggtagtcata tgcagaaaag aactttcaaa
     1081 aaaaccggtg aaaaaatctc tcttctcggt atgggagtaa tgcgtcttcc cgaaacatca
     1141 ggcggtctga tcgatgaaga tctgtctgta aaaataatca gaagagccat agacggcggt
     1201 gtcagttacg tggactgtgc ctacacatac cacggcggag ccagtgaaaa ggtccttggc
     1261 aaagccctga aaaacggtta cagggaaaag gtcttcatag ccgacaagat accctcatgg
     1321 ttggccaaag atgaaaaaga tatcgaaaaa atgttcgaag aaagctttgc ccgcctggat
     1381 gttgatttca ttgacatgta tcttatccat aacatcactg cccccatatg gaaaaaagtc
     1441 caaaagctga acgttatccc tttcctggaa caaaaacaga aggaaggaaa gatcggacat
     1501 ctgggatttt cttttcatga cagcctttct cttttcaagg aagtcgtaaa cttccgccgc
     1561 tgggatttct gccagataca gctcaactat atggattctg atatccaggc cggagtggaa
     1621 ggccttcgtt atgcggcttc aaaaggtctt tccgttatta taatggagcc tctaaaggga
     1681 ggcaggctta cggataccgt tccttcatcc ataaaagaaa tatgggacag agccgaaaca
     1741 aaaagatctc ctgcggaatg gtgtttccgc tgggtggccg attttcctga ggtgaccacc
     1801 atattaagcg gcatgagcac aatggaacag ctggaggaga atctgaaaat aatcgaaaac
     1861 tgccttcccg aaagtcttac agaaaatgaa cattccctga tcgaagaagt ggcggacaag
     1921 tatagggaac ttatcaaata ccagtgtacc gactgcaaat actgtatgcc ctgccctttc
     1981 aaggtggata tcccccggtg cctttctctg tataatgaat ggtttcttta cgataaaaat
     2041 ccaaagaccc ttatggagta caagaactgg atggataaaa ggcagagggc ttccaactgc
     2101 gcggactgta aagagtgtga aagcaaatgc ccccagcagc tgcatataac agaagccatg
     2161 aaaaagataa aaaatgaatt cggggaatga ggcacgatca aacaaggttc atcaatcata
     2221 accacccttc caaagggtgg tttgctctag ggctataagc cctggct
//
LOCUS       DAONHK010000160         2248 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_41726_length_2248_cov_4.375741, whole genome shotgun sequence.
ACCESSION   DAONHK010000160 DAONHK010000000
VERSION     DAONHK010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41726_length_2248_cov_4.375741"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..649)
                     /locus_tag="PLM92_05500"
     CDS             complement(<1..649)
                     /locus_tag="PLM92_05500"
                     /inference="COORDINATES: protein motif:HMM:NF015181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HPR24626.1"
                     /translation="MKQIMIKGVHNTGKTTTVTAVIKELIKRGYTVGSVKDIHFVGFA
                     IDEEGTDTWKHADAGATTVTARGLEETDIIHKKKLDLESVLCQYDQDYLVIEGDPGAS
                     CPNIVTGEKTEDLDERMDERTIAFSGIIGDSLQCYKGLPVMSNKKDIKKMVDLIEKEV
                     KIMADARKETEVKMYFDDKEVPMVPFVEAIIRGSIMGIAKELKGYEEGCEVKIVMK"
     gene            complement(669..2060)
                     /locus_tag="PLM92_05505"
     CDS             complement(669..2060)
                     /locus_tag="PLM92_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPR24627.1"
                     /translation="MSDIKINMDRSTGQIKMQELNDQMVRIIKKDFPGHIKSGRELAA
                     YVASATAEYNGKLPIYVMYMPKLFTEKEGAFLKQTAETMHGILKRMIEEYIENPDYRP
                     LFGFSKQAEELIMTPSFYDSLLPMARVDIFLNEEDMSFKFCEFNSDGSSGMNEDREFV
                     NGFKDTLLYRELSKEYSLQPFELFDTWCETVKRIYGTFKNRVPEPRTGIVDFTEKGCS
                     PEEFNAFREAFIRNGMDAVICEIRDMKYRGGILYDGEGRRIDIVYRRAVTRDVLEKSD
                     EIKDFLAAVKDGNICLIGSFATQVIHDKIAFVNLHLKETQSFLTGEQNDFVRKHVPYT
                     AELKKEKTDIDEIIETKDKWLIKPRDSYGAVDIYAGNKFKTDEWAGIVKKYEGTDFLV
                     QEFCTPYRTRNLDCQLKEPGFINYSNLTGLFLYDGVFSGAYSRISDDTIIAEQYDEND
                     IGSYIARALKQNI"
     gene            complement(2053..>2248)
                     /locus_tag="PLM92_05510"
     CDS             complement(2053..>2248)
                     /locus_tag="PLM92_05510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPR24628.1"
                     /translation="EKGYDAVVYGIPVCEIFDEMIYHMSINLSREEKHYTDIMSDMIR
                     RRENPAMMMKKDHKKGDRHV"
BASE COUNT          503 a          556 c          376 g          813 t
ORIGIN      
        1 atttcatcac tattttgact tcacagccct cttcatatcc tttgagttct tttgcgatac
       61 ccattatgct gcctcttatt attgcttcaa caaacgggac catcggaact tccttatcgt
      121 caaaatacat tttgacttca gtttcctttc ttgcatctgc cattattttt acctcctttt
      181 caatcaaatc aaccattttt tttatatctt ttttattgct cattacgggc agtcctttgt
      241 agcattgaag gctgtctcct attatgcctg aaaaagcaat agttctttca tccattctct
      301 cgtcaagatc ttctgttttt tctccggtaa ctatgttggg gcaggatgct cccggatcgc
      361 cttctattac cagatagtcc tgatcatact ggcaaagcac actttcaaga tcaagctttt
      421 tcttgtggat tatatccgtt tcctcaagac cccttgccgt cactgtggtg gctccggcat
      481 ctgcatgctt ccatgtgtct gttccttctt cgtctattgc aaaccccaca aaatgtatgt
      541 ctttcaccga ccccaccgta tatccgcgtt ttatcagctc ttttatgact gctgtgaccg
      601 tggtggtttt acctgtatta tggacccctt ttatcattat ctgcttcatt aagtactctc
      661 ctttcaggtt atatgttttg cttaagtgcc cgggcaatgt aggagcctat gtcattttcg
      721 tcatactgct cggcaattat ggtatcgtct gatatcctgg aataggctcc gctgaatacg
      781 ccgtcataaa ggaaaagccc tgtaagattg ctgtagttta taaacccggg ttcctttagc
      841 tgacaatcca gattccgggt cctgtagggc gtgcagaatt cctgcaccag gaaatcagtc
      901 ccctcgtact tttttactat tcctgcccat tcatccgttt tgaatttgtt tcctgcgtaa
      961 atatccaccg ctccgtagga atctctgggc tttatgagcc atttgtcttt tgtctctatt
     1021 atttcgtcaa tatctgtctt ttcttttttt aattccgccg tataagggac atgctttctt
     1081 acaaaatcat tctgctctcc cgtgagaaag ctttgggtct ctttcagatg cagattgaca
     1141 aatgctattt tgtcatgtat cacttgtgtt gcaaaactac cgatcagaca aatatttccg
     1201 tctttcactg ccgcaagaaa atcctttatt tcatcacttt tctcgagaac atctctggtc
     1261 actgcccttc tgtatactat gtctatcctt ctgccttcac cgtcatagag gatgcctccc
     1321 ctgtatttca tatctcttat ctcgcagatg acagcatcca tgccgtttct tatgaaggcc
     1381 tccctgaacg cattgaactc ctcaggagag cagcccttct ctgtgaaatc aactattcct
     1441 gtcctgggct ccggaactct attcttgaag gttccatata tcctttttac ggtttcgcac
     1501 catgtatcaa aaagttcaaa gggctgaaga ctgtattctt tgctgagctc cctgtacagc
     1561 agtgtgtctt tgaatccgtt cacgaactcc ctgtcttcgt tcatgccgct gctgccgtcg
     1621 gaattgaatt cacagaattt aaatgacatg tcttcttcgt tcagaaatat atcaactctg
     1681 gccattggca gcaggctgtc ataaaatgac ggtgtcatta taagttcctc tgcctgtttc
     1741 gaaaaaccga aaagaggtct gtaatccggg ttctctatat actcctctat cattctcttc
     1801 aggatgccgt gcatggtctc tgcagtttgc ttaagaaaag ccccttcttt ttctgtaaaa
     1861 agctttggca tatacatcac atatatagga agttttccat tgtattcagc agtggccgat
     1921 gccacatatg ccgccagttc tctgccgctt tttatatgtc ccgggaaatc cttttttatt
     1981 atgcggacca tttgatcatt cagttcctgc atttttattt gcccggtaga tctgtccatg
     2041 tttatcttta tatcagacat gtctgtcacc ttttttatga tcttttttca tcatcatagc
     2101 cggattttct cttcttctta tcatatcaga cattatatcc gtataatgtt tttcctctcg
     2161 ggaaagattt atactcatat gatatatcat ttcatcaaat atttcacata ctggaatacc
     2221 ataaacaact gcatcatatc ccttctct
//
LOCUS       DAONHK010000161         2217 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_42663_length_2217_cov_3.104533, whole genome shotgun sequence.
ACCESSION   DAONHK010000161 DAONHK010000000
VERSION     DAONHK010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42663_length_2217_cov_3.104533"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..646)
                     /locus_tag="PLM92_05515"
     CDS             complement(<1..646)
                     /locus_tag="PLM92_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005213549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPR24629.1"
                     /translation="MKKILIIEDEPNIRELVTYNLKTNGYEPLESEDGITGITLVYKE
                     KPDLILLDIMLPGKDGYDICRELRSEGNNTPIIMLTAKSEEVDKVLGLEFGADDYISK
                     PFGVRELMARIKAVLRRYDLSEEEVDVKKSEDTVIAGDIRINFDRHEVLVCEEKVELT
                     MKEFELLGFLARNRGIAFSRDELLDRVWGIDYPGETRTIDVHIRYIRKKLGNGAD"
     gene            complement(727..>2217)
                     /locus_tag="PLM92_05520"
     CDS             complement(727..>2217)
                     /locus_tag="PLM92_05520"
                     /inference="COORDINATES: protein motif:HMM:NF037997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HPR24630.1"
                     /translation="IGFVSAGLMKLPQAISVILGANIGTTVTAQLIAFKIGDYAWVFV
                     FVGFIMFFFIKNKEQVKNVGQTLFAFGLLFVGINTMSAVMKPMAASPVFADMMMQVQD
                     IPFLGIIIGTLMTIVVQSSSATIAVLQNLASTAGPDGVTSIIGLQGSLPILFGDNIGT
                     TITALLASIGASVNAKRTALAHTIFNVTGTLLFIWFIPQIARLIQFISPKGPEIEVIA
                     RQIANAHLFFNLTNTLIWLPFIWVLALIVTKILPGRDSDKLTSEPIYLDFKIIDQSVF
                     AIHLATKELYRIAEFAYDMMVKAKKAFIGNDTDAVRDVFEIEDTVNSLQEKTVNYLAS
                     IFSSESVTESQKVRLSGLLHVAADIEHIGDHCKNIAEFAQEKTVNKYDFSDTALAEIY
                     ECFDQAKRMMKDSMKALETGDRNIAKDILKQEEAMNRTEIRLRKKHMQRLDEKTCSPA
                     FTVIYTDVVHNIEKIGDCCNNIAEAVLEDVHFKSEFDREDSFAENT"
BASE COUNT          576 a          498 c          417 g          726 t
ORIGIN      
        1 cgtctgcacc attgcccagc ttctttctga tatatcttat atgtacatct atcgtgcggg
       61 tctctccggg ataatcgatc ccccaaactc tgtccagaag ttcatctctt gaaaaagcta
      121 tgcctctgtt ccgggcaaga aatcccagaa gttcgaactc tttcatggtg agttccactt
      181 tttcttcgca gacaagcacc tcatgcctgt caaagtttat tcttatatct cctgcaataa
      241 ccgtatcttc gctttttttc acatctactt cttcttctga taaatcatat cttctcaata
      301 cagcttttat cctggccatc agttctctga ccccaaaggg ctttgatata taatcatccg
      361 caccgaattc aagacccagt accttgtcaa cttcctccga tttggcggtg agcattatta
      421 tgggagtatt gtttccttcc gaacgcagtt cacggcatat atcatatcca tccttgcccg
      481 gtagcataat atccagaagt atcagatcgg gtttttcttt atataccaga gtaatgcctg
      541 taattccgtc ttctgattcc aggggttcat atccgttagt cttaagatta tatgttacca
      601 gttctcttat gttgggctca tcttctataa taaggatttt tttcatatta tgcttccttc
      661 cccgggacat aaatattatg agaattttca caataatatt ttaacacatc ttcggttttg
      721 tcacaatcat gtgttttccg caaaagagtc ctcccggtca aattccgatt tgaaatgaac
      781 atcctcaaga acggcttcag caatattgtt gcaacagtct cctatttttt ctatgttatg
      841 aacaacatct gtgtatatta cagtaaaagc gggagaacag gttttttcgt caagtctttg
      901 catgtgtttt tttcgcagtc ttatctcggt cctgttcata gcctcttcct gtttgaggat
      961 atcttttgct atatttctgt ctcctgtctc aagggccttc atggagtctt tcatcattct
     1021 ttttgcctga tcgaaacatt cgtatatctc cgcaagagca gtatcggaaa agtcatattt
     1081 attaactgtt ttttcctgag caaattctgc tatgttcttg cagtgatcac cgatatgttc
     1141 tatgtcggca gccacatgaa gcagtcccga aagtcttact ttctgacttt ctgtaacgga
     1201 ttccgatgaa aatatggaag caagataatt cactgttttt tcctgcagag aattcacagt
     1261 gtcttctatt tcaaaaacat ccctcaccgc atctgtatcg tttcctataa atgctttttt
     1321 tgctttgacc atcatgtcat atgcaaattc tgcgatcctg taaagttcct ttgttgcaag
     1381 atgtattgca aatacagact gatctattat tttgaagtca agatatatgg gttctgatgt
     1441 cagtttgtcc gaatctctgc cgggaaggat cttggtcact atcagtgcca gcacccaaat
     1501 gaacggaagc cagataagcg tattggtaag gttgaagaac aggtgtgcat tggctatctg
     1561 ccttgctatg acttctatct caggcccttt tggagaaatg aactgtatca gtcttgctat
     1621 ctgaggtatg aaccatataa agagaagtgt accggtaaca ttgaaaatgg tatgagcaag
     1681 agcagttctc ttggcattga cagatgcacc gatggatgcc agaagggcgg ttattgttgt
     1741 gccgatatta tctccgaaaa gtataggaag tgaaccctga aggcctataa tactggtcac
     1801 cccatcgggg cctgctgtgg atgccaggtt ctgaagcaca gctatggtgg cggaactgct
     1861 ctgcaccact atcgtcatca aagtaccaat gattatcccg agaaaaggta tgtcctgcac
     1921 ctgcatcatc atgtctgcaa atacaggcga tgctgccatg ggtttcatga cggcactcat
     1981 ggtatttatc cctacgaaca gaagcccgaa agcaaaaagt gtctgtccca cattcttgac
     2041 ctgttcttta ttttttataa aaaagaacat tataaatcca acaaaaacga atacccatgc
     2101 ataatccccg attttgaatg caatgagctg tgcagttacc gtagttccta tgtttgctcc
     2161 gagtataacg ctaatggcct gaggaagttt cataagtcct gcactgacaa atcctat
//
LOCUS       DAONHK010000162         2160 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_44378_length_2160_cov_5.221853, whole genome shotgun sequence.
ACCESSION   DAONHK010000162 DAONHK010000000
VERSION     DAONHK010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44378_length_2160_cov_5.221853"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1368)
                     /locus_tag="PLM92_05525"
     CDS             complement(<1..1368)
                     /locus_tag="PLM92_05525"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HPR24631.1"
                     /translation="MKSPAIRSRKRIIFAFGLFCALLLAVCIKIGYIQIVQGEELTKK
                     AIAQQTNDEAIEAKRGEIRDRNGKELAVSTICYSLWARPAAMEDKDSAAVTLAEITDM
                     DAEEIKEMVNQDKSLVKIAKYLDSGAAEEIRKADMPGVSITEESKRSYPLGEFASHLL
                     GSVTDDNNGLTGLEQKYNGYLKGVSGRWLKNTDASGNPLAYGADKYYEAENGATLLLT
                     IDEVLQHYTEKAIASVAKTTKADRVSCLIMDPETGEILAAASTPGFDPNYPRIPTDEV
                     EAKKLGSMNEKKQMEYLNEMWRDPLFNDTYEPGSTAKLVTTSIALEEGLTDTSENFVC
                     TGSIELYGETLKCWRSENPHGKQTLTEAVGNSCNPVFVTLSTRIGLEKYYKYLELFGL
                     TQKTGVDFPGEANSILQNKETAGPIGLATMAYGQGIAMTPVQLLTATCALGNEGKLMR
                     PRLVKE"
     gene            complement(1472..1903)
                     /locus_tag="PLM92_05530"
     CDS             complement(1472..1903)
                     /locus_tag="PLM92_05530"
                     /inference="COORDINATES: protein motif:HMM:NF016860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HPR24632.1"
                     /translation="MITAEQWYEQQTNYTKYGFDMKPRASSERPGNSSGVTVADRKRL
                     MFLVVVAGLICIGIIISSAYAASVAFTNNQLKEQNAALQSEVETLEIKIQSANNIRTI
                     EEKATGELGMVYPEGEQYVVLSGEEKTADDFGSLLKKQAFQ"
     gene            complement(1878..>2160)
                     /gene="mraW"
                     /locus_tag="PLM92_05535"
     CDS             complement(1878..>2160)
                     /gene="mraW"
                     /locus_tag="PLM92_05535"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015541976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="16S rRNA
                     (cytosine(1402)-N(4))-methyltransferase"
                     /protein_id="HPR24633.1"
                     /translation="DVLADKGRLCVITFHSLEDRIVKETINKRADPCTCPPEFPVCVC
                     GKKADIVKITKKPVIPDEAEIEANPRSRSAKLRVAEKRVKSDDNSRTMV"
BASE COUNT          442 a          557 c          433 g          728 t
ORIGIN      
        1 ctctttgacc agtctcggtc tcattagttt cccctcgttt ccaagggcac aggtcgccgt
       61 caaaagctgc accggggtca tggctatgcc ctgaccgtat gccatagtag ccagtcctat
      121 gggacctgct gtttctttat tctgaagtat ggaattcgcc tcgcccggga aatcgacccc
      181 cgttttctga gtgagcccga aaagctccag atatttatag tatttttcca ggcctatcct
      241 ggtagataat gtcacaaaga caggattgca tgagtttccc accgcctctg taagggtctg
      301 cttgccgtgg ggattttcgg aacgccagca cttaagtgtt tctccgtaaa gctctatgga
      361 gccggtgcag acgaaattct ccgatgtgtc agtcaggcct tcctccagtg ctatagatgt
      421 ggtgaccagt tttgctgtgg aacccggctc atatgtatcg ttaaaaagcg gatctctcca
      481 catttcattc agatattcca tttgcttctt ttcattcatg gatccgagct ttttggcttc
      541 gacttcatcg gtgggtattc tgggataatt gggatcaaac cccggagtag aggccgctgc
      601 cagtatctct ccggtttcgg gatccattat aagacaactg accctgtccg cctttgtggt
      661 ttttgccaca gaagcgatgg ctttttctgt gtaatgctgc agcacctcat ctatggtcaa
      721 aagaagagtg gctccgtttt cagcctcata atacttgtct gcgccgtagg caagaggatt
      781 cccggaggca tctgtatttt tcagccatct tcctgacact cccttaagat agccgttgta
      841 tttctgctca aggcccgtca gcccgttgtt gtcgtcagtc acgcttccca gcagatggga
      901 tgcgaattct cccaggggat acgatctttt gctttcttct gttatggaca ccccgggcat
      961 atctgctttt cttatttctt ctgcagcccc cgagtcaaga tatttggcga tttttaccaa
     1021 acttttgtcc tggttgacca tttcttttat ttcttccgca tccatatccg ttatctctgc
     1081 cagagtcacg gctgcggaat ctttatcctc cattgccgcc ggtcttgccc aaagggaata
     1141 gcagatggta ctgactgcca gttcctttcc gtttctgtct cttatttccc ctctttttgc
     1201 ttctatggcc tcatcattgg tctgctgtgc tattgctttt tttgtcagtt cttctccctg
     1261 tactatctgt atatatccaa tttttataca cacagcaaga agaagcgcac agaacagccc
     1321 gaatgcaaat ataatccttt ttctgcttct tatggccggt gatttcattt ttttgccttt
     1381 ctttccgctt ttctaataaa aaagtcagac aaacactttt ttcctcatta agttgtctga
     1441 ccgatccttt ttattcagtt actgctgccg ttcactggaa agcctgtttt ttcagtaggc
     1501 ttccaaaatc atcagcagtt ttctcttctc ccgacaagac aacatactgc tcaccctcgg
     1561 gataaaccat gccaagttca ccggtggctt tttcttcaat ggttcttatg ttattggcac
     1621 tttgtatctt tatttccaga gtttcgactt cgctttgaag agctgcattc tgttctttca
     1681 gctgattgtt ggtaaatgcc acagacgctg catatgcgga agatattatt atgccaatac
     1741 aaataagccc cgcgaccacc accaggaaca taagcctttt cctgtcagct accgttacac
     1801 cgctgctgtt tccgggtctt tccgaggagg ctctcggctt catgtcaaat ccgtattttg
     1861 tataatttgt ctgttgttca taccattgtt ctgctgttat catcactctt cacccttttc
     1921 tccgccaccc ttagtttcgc gctcctggat ctggggtttg cttcaatttc cgcttcatca
     1981 ggtatcacag gtttttttgt tattttcact atgtccgctt tctttccgca aacgcatacc
     2041 ggaaattccg gtggacatgt acatggatca gcccttttgt ttatagtttc ttttactatt
     2101 ctgtcttcaa gagaatgaaa ggttatcacg caaagccttc ctttatcagc cagaacgtca
//
LOCUS       DAONHK010000163         2159 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_44411_length_2159_cov_3.800380, whole genome shotgun sequence.
ACCESSION   DAONHK010000163 DAONHK010000000
VERSION     DAONHK010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44411_length_2159_cov_3.800380"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /gene="ybeY"
                     /locus_tag="PLM92_05540"
     CDS             <1..248
                     /gene="ybeY"
                     /locus_tag="PLM92_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009530202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HPR24634.1"
                     /translation="QYDTQEEIPKTGMSVLGDIVICSPIAKKQAEEAGHSEKRELVYL
                     FVHSMLHLLGHDHMKKEEKKLMRETEEKIMHSIDMER"
     gene            257..631
                     /gene="cdd"
                     /locus_tag="PLM92_05545"
     CDS             257..631
                     /gene="cdd"
                     /locus_tag="PLM92_05545"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018598611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HPR24635.1"
                     /translation="MYRNLYKEAKTMINNAYAPYSRFKVGAALMTKDGKIFKGANIEN
                     ASFGAGICAEQVAVSAAVSAGEKEFDAIAVVSGEGSAWPCGICRQFMYEFSPEMKVIT
                     GDDEDHLEVYELAELLPKGFVL"
     gene            628..1509
                     /gene="era"
                     /locus_tag="PLM92_05550"
     CDS             628..1509
                     /gene="era"
                     /locus_tag="PLM92_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009644773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HPR24636.1"
                     /translation="MKSGFIGIIGRPNVGKSTLLNSILGEKIAITTAKPQTTRNVIRG
                     IYTGDDIQMVFIDTPGITKPRNKLGEFMTGSAIRTLNEVDIIIFIVDDYPEKGPGDGY
                     ITDILKEINTPKILLINKIDIMDPQKYKGIYDFYEKTAIFEEILGVSAKDGTNVRDII
                     DHTGRMLDEGPMYFPEDTITDHPERFIVSEIIREKLLLYLEEEVPHGVAVEIESYEEK
                     PDIIRIGAVIYCEKKSHKGIIIGKEGRKLKGVGKSARQEIESLLGTKVFLELWVKIKE
                     NWRDSDRDLSSFGYKVK"
     gene            1509..>2159
                     /gene="recO"
                     /locus_tag="PLM92_05555"
     CDS             1509..>2159
                     /gene="recO"
                     /locus_tag="PLM92_05555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HPR24637.1"
                     /translation="MYTDTEGIILRQTKTVNGRRMVLLFSKKYGKISAGTGISEKGRN
                     KAALAMRPFAYGRYEIFKSRQNFNIKGAEVLKSYYKIGEDVDKYMNSSYILELTEKLL
                     AEEQQNTRMFDLLLDFFDAMEKRKKKYMTLVLAYETKALGILGYMPELSVCTICGKKN
                     EPALLDIKEGGIVCRDCVNNAGHHDRHTLIYDIDFGIVDILKYFSANSLKHLENLAL"
BASE COUNT          777 a          337 c          522 g          523 t
ORIGIN      
        1 cccaatatga cactcaggaa gaaataccca aaaccggcat gtcagtactg ggagatatag
       61 tgatatgtag tcccatagca aaaaagcagg cggaagaagc gggacattcg gaaaaaagag
      121 agttggtata tctttttgtt cacagcatgc ttcatttgct tggacatgat catatgaaaa
      181 aagaagaaaa aaagctgatg agagaaaccg aagaaaaaat aatgcacagc atcgatatgg
      241 aaaggtaggg gatatcatgt acaggaatct ttataaagaa gcaaaaacta tgatcaacaa
      301 cgcatatgca ccatattcca gattcaaagt gggtgcagcc ttaatgacaa aggacggaaa
      361 aatattcaag ggagccaata tagaaaatgc atcattcggt gccggaatat gtgcggagca
      421 ggttgctgta tcggcagctg tttcggcggg agagaaggag tttgatgcta ttgcggtggt
      481 ttccggagaa ggaagtgcat ggccctgtgg tatatgcaga cagttcatgt atgagttcag
      541 ccctgaaatg aaagttatta ccggagacga tgaagaccat ctggaagtat atgaactggc
      601 tgagcttctg ccgaaaggat ttgtgctgtg aaaagtggat ttataggtat tatcggaaga
      661 cccaatgtgg ggaaatcaac acttcttaat tccatactgg gagaaaaaat agccatcaca
      721 acggccaagc cgcaaacaac aagaaatgtt atcagaggaa tatacaccgg cgatgatata
      781 cagatggttt ttattgatac tcccggaata acaaaaccta gaaataagct gggagaattc
      841 atgacaggaa gcgccataag gacgctgaat gaagttgaca taattatatt tattgtggat
      901 gattatccgg aaaagggtcc gggagatgga tatataacgg atattctaaa agaaataaat
      961 actcccaaga tacttcttat aaacaagata gatatcatgg atccgcaaaa gtataaggga
     1021 atatatgatt tttatgagaa aaccgctata tttgaagaaa tacttggggt gtcggctaaa
     1081 gacggtacaa atgtcagaga tatcattgat catacaggca ggatgcttga tgaagggccc
     1141 atgtatttcc ctgaggacac tataacagat catcctgaaa gattcatcgt gagtgagata
     1201 ataagggaaa aactcctatt gtatctggag gaggaagtgc cccatggagt cgcggtggag
     1261 atagagtcct atgaagaaaa accggacata ataagaatag gggctgtcat atactgtgaa
     1321 aaaaaatctc ataaaggtat tataattgga aaagaaggaa ggaaactgaa aggtgtcggc
     1381 aaaagtgcaa gacaggaaat cgaatccctt ctgggaacaa aagttttttt agagctttgg
     1441 gtaaagatca aagaaaactg gagagacagc gacagggatc tttcctcttt tggatataag
     1501 gtaaaataat gtatacagat acagaaggga tcatcttaag acagacaaaa accgtcaatg
     1561 gaagaaggat ggttcttttg ttttcaaaaa agtacggcaa aataagtgcc ggtaccggaa
     1621 taagtgaaaa aggaagaaat aaagcggcat tggcaatgag accttttgcc tacggcagat
     1681 atgagatttt taaaagcagg cagaatttca atataaaagg tgccgaagtc ctgaaaagct
     1741 attacaagat aggggaagat gtggacaaat acatgaacag ttcatatata ctggagctca
     1801 ctgagaaact gcttgccgaa gaacagcaga acaccagaat gttcgatctt cttctggatt
     1861 tttttgatgc catggaaaag cggaaaaaaa agtatatgac cctggtcctt gcctatgaaa
     1921 caaaggccct tggtatactg ggttatatgc cggagctttc agtatgcacc atatgcggaa
     1981 agaagaatga acccgcctta cttgatataa aagaaggagg gatagtatgc agggactgtg
     2041 taaataatgc cggtcatcat gataggcata cattgattta tgacattgat tttggtatag
     2101 tagatatatt aaaatatttt tcggcaaact cattgaagca tcttgaaaat ctggctctg
//
LOCUS       DAONHK010000164         2134 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_45205_length_2134_cov_2.668591, whole genome shotgun sequence.
ACCESSION   DAONHK010000164 DAONHK010000000
VERSION     DAONHK010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45205_length_2134_cov_2.668591"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(418..825)
                     /locus_tag="PLM92_05560"
     CDS             complement(418..825)
                     /locus_tag="PLM92_05560"
                     /inference="COORDINATES: protein motif:HMM:NF020376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HPR24638.1"
                     /translation="MCDFFVDEENEEDNDEDKDEDEEESEVIYLSGVALSGTVNKKGI
                     TELISRIKIFDRLFFFKENAENSEKDLIAVRTEEGERIGHVPEHKALLIDMMMDAGKR
                     VYGRLEKKEPMGRGFKIVIGVYAREKKVPKRTG"
     gene            complement(854..1162)
                     /locus_tag="PLM92_05565"
     CDS             complement(854..1162)
                     /locus_tag="PLM92_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004632166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HPR24639.1"
                     /translation="MKPKHITITGLSYHGDDRFLYRPGRVFKCVKEKENPYDEEAIGV
                     QLEGIKIRGYVANSVNTVAKGTMSAGRIYDKTDNEFDIIVRFVTRASVICEVIEETEK
                     "
     gene            1294..>2134
                     /locus_tag="PLM92_05570"
     CDS             1294..>2134
                     /locus_tag="PLM92_05570"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HPR24640.1"
                     /translation="MEKTKLKMLKIMDILRETDEEHPITAGQICGKLQAEGISAERKS
                     VSSDIRLLMEYGIDIIMCADKKNGCYMASRQFEDWELKILIDAVWQAKCLTRNVSKTL
                     VDKLLATTCKESRQTLKQATYIFSHAKTRNKTTQIAIDVFLRALIKGRKVSFQYTYIG
                     PDLKSHLRKDGCLYTVSPYSLVWGNDFYYLICNTDGHEGLTWYRLDRMKNFLLTDCPS
                     RPACETAGPDPNYAISEYVKNSIHHYGGKKIHLQLRTCESMIDHLLDQFGDNIIIKKE
                     ENKK"
BASE COUNT          579 a          494 c          363 g          698 t
ORIGIN      
        1 tcctttgtca attttaaatt ttgactgtat ggtttcaatc cattagtatg tagcttttcc
       61 acatactctg tttctatttt tcccataagg aacccgtgga aaagtgatga catattataa
      121 tttattggtt cctcattcgt ttctattctg atttcaattt tttttaacaa ttgacttgct
      181 cctttactgt acactttgat ttgctcataa acatctcccg tttgattatg caaaaaaaac
      241 aggcaccaaa aaaattgtgc ctgttgactt atcacttatt ttggatgctt tttcatgcag
      301 tcctccctgt gatacgagaa atatgtttta ttattataca ttataacact tttattcaaa
      361 attttcactt atttatattt tctcccattg ttgcctgctt ttttagggtt ccctttgcta
      421 tcctgttctc ttcggcactt tcttttccct ggcatataca cctattacta ttttgaaacc
      481 tctccccatg ggttcctttt tttcaagcct gccgtagact cttttgcctg catccatcat
      541 catatcgatg agaagcgctt tgtgctcagg tacatgaccg atcctttctc cttcttcggt
      601 cctgactgct atcagatcct tttcactgtt ttctgcattc tctttgaaga aaaacagtct
      661 gtcaaaaatt tttatccggg atataagttc agtgattcct tttttgttta ctgttcccga
      721 cagagcaaca ccggaaagat aaatgacttc gctctcctct tcgtcttcat ctttatcttc
      781 atcattatct tcttcatttt cttcatcaac gaaaaaatca cacacttttg atcatctcct
      841 ttttgcccca tctctatttt tcagtttctt caatgacttc gcatataaca cttgcccttg
      901 ttacaaaacg aacgattata tcgaattcat tatctgtttt gtcatatatc ctgcctgcac
      961 tcatggttcc tttagccacg gtattgacac tgttggccac gtatcccctt atctttattc
     1021 cttccagctg aacaccgatg gcctcttcat cataggggtt ttctttttct tttacacact
     1081 taaaaactct tcccggtctg taaagaaacc ggtcgtcccc atgatatgaa agtccggtta
     1141 tggtaatatg ttttggcttc attactattc tccttcctct tctgtgaacg cattatagca
     1201 cgaaaaccgg ctttcacttg tcctttttct tggtcaggtg atatgttata atgtgcatat
     1261 ataaaatcaa gatccactca cggaggtacc cacatggaaa aaacaaaatt aaaaatgctc
     1321 aagataatgg acatattaag agaaacagat gaggaacacc ccatcacggc cggacagata
     1381 tgcggaaagc tccaggcaga aggcataagc gcagaaagaa aatcggtcag cagtgatata
     1441 cggctgctta tggaatacgg cattgatata atcatgtgcg cagacaagaa aaatggctgc
     1501 tacatggcaa gccgtcagtt cgaggactgg gagctcaaga tacttatcga tgcagtctgg
     1561 caggccaagt gtctcactcg aaatgtgtca aagactttag tagataaact gctggccacc
     1621 acgtgtaaag aaagccgaca aactcttaaa caggcaactt atattttttc ccatgcaaaa
     1681 acaagaaaca agactaccca gattgccata gatgtcttcc tccgggcatt gataaagggc
     1741 cggaaggttt ctttccagta tacatatatt ggtccggacc tcaagtctca tcttcgcaaa
     1801 gacggctgcc tttatacagt cagcccctac tcccttgtct ggggcaatga tttttattat
     1861 ctgatatgca acaccgatgg ccatgaaggc cttacctggt atcgcctgga ccggatgaag
     1921 aattttttgc tgactgactg tccttcccgt ccggcttgcg aaacggcggg cccggatccc
     1981 aactatgcca tttctgagta tgtcaaaaat agtatccatc actacggagg caaaaaaata
     2041 catctccagc tgcgcacctg cgaaagtatg atagatcatc ttctggacca gttcggggat
     2101 aatatcataa taaaaaaaga agaaaacaaa aaaa
//
LOCUS       DAONHK010000165         2132 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_45265_length_2132_cov_3.058257, whole genome shotgun sequence.
ACCESSION   DAONHK010000165 DAONHK010000000
VERSION     DAONHK010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45265_length_2132_cov_3.058257"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(133..1293)
                     /locus_tag="PLM92_05575"
     CDS             complement(133..1293)
                     /locus_tag="PLM92_05575"
                     /inference="COORDINATES: protein motif:HMM:NF016357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047355 - CDP-glycerol
                     glycerophosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HPR24641.1"
                     /translation="MKNLIKKIKGVINAVIFIIINRVLCFFTKVEEKKVLFASDVRSG
                     TGGNYSFILEWLAGKGYEVIYDFKADRRVKRGLRGSLALVYKMTTSGSILLEDYFRYI
                     TFMKVRKGQQICQLWHGAGAFKKFGFSRAAENEGIRIHKGYRKYAKAIVSAEEIRGCY
                     AEAFRIPKENVRATGIPRTDIFFDEKYIAGKKEELIKEFPVFEKKKVILFAPTYRGVR
                     AEDAAYDFDMLDLKKLYDDLNEEHVFVFKWHPAAYNNIMMNNREVYDLKKYGDFFLDL
                     SSDRDINDLLLVTDILITDYSSVIFDYLLVDRPIVYYPYDLAAYEAGRGLYYDYEAYL
                     YGTVAMDQKELTDAIKAGDMCSEKREAFRKHFMEACDGHSTEKTCRWFFEEE"
     gene            1410..>2132
                     /locus_tag="PLM92_05580"
     CDS             1410..>2132
                     /locus_tag="PLM92_05580"
                     /inference="COORDINATES: protein motif:HMM:NF016145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HPR24642.1"
                     /translation="MKNLYIRAGMAPFENLDPYHVLLHNSISGNSGNLLYAYGLCRAI
                     MTENTNIEVDHYTTSSSDMPDINKKYDAWIIPLADAFRNNFTEVMDKLTKCIRKSRIP
                     VILVGVGLRAPLETDLSSSFPFDDHVKKFVSAILDKSAVIGVRGETTGRYLTKLGFRE
                     GSHYKVIGCPSMYGFGDTLKIREPQINKGSLISLNSSIFSEQKTIDFVERVRQQFPNH
                     YFVPQHRRELAEYHGFSCVYQKD"
BASE COUNT          580 a          488 c          408 g          656 t
ORIGIN      
        1 aaaaatccag ggacggctca tccccctggc gactcagtat ggttatctgc cgctttgtgg
       61 gcgccagttt atgaaagaaa tatatgaagt taagtatgcc ccttaccagg gcaagaaaaa
      121 atctcattat tttcattcct cctcaaaaaa ccagcggcat gttttttctg ttgaatgtcc
      181 gtcacaggcc tccatgaaat gcttcctgaa ggcttctctt ttttcgctgc acatatcccc
      241 ggcttttatg gcatctgtca gctccttctg atccatggca acagtgccgt aaagataagc
      301 ttcataatca taataaagac ctctgccggc ttcataggca gcaagatcat aggggtaata
      361 gactatgggt ctatccacca gaaggtaatc gaatataaca gatgaataat ccgttatcag
      421 tatatctgta acaagcagca gatcgtttat gtctctgtcc gaagaaagat ccaggaaaaa
      481 atctccgtat tttttgagat catatacttc cctgttgttc atcatgatat tgttgtaggc
      541 tgccggatgc catttgaata caaaaacatg ttcttcatta aggtcatcat aaagcttttt
      601 taaatcaagc atatcaaagt catatgctgc atcttctgct cttacccccc tgtatgtggg
      661 agcgaacagt atcacttttt tcttttcaaa taccgggaat tcttttataa gttcttcttt
      721 tttccccgct atatattttt cgtcaaagaa gatatcggtc ctgggtattc ccgttgccct
      781 tacattttct tttggtatcc ggaatgcctc cgcatagcat ccccttattt cctcggcact
      841 cactatggct ttggcatact tgcggtaccc tttgtgtatc cttatgccct cattttctgc
      901 cgcccggctg aatccgaatt ttttgaaggc tccggcccca tgccacagct ggcatatctg
      961 ctgtcctttt ctgactttca taaaggttat ataccggaaa taatcctcaa gcaggatgct
     1021 tccggaagta gtcattttat ataccagtgc gagggatcct ctaaggcctc ttttgacccg
     1081 tctgtccgcc ttgaaatcat atatcacttc atatcctttg ccggcgagcc attcaaggat
     1141 aaaactgtaa tttccgccgg tcccggatct gacatcggaa gcaaaaagga cctttttttc
     1201 ttccactttc gtaaaaaaac aaaggacacg gtttatgata atgaatatga cggcatttat
     1261 aacgcctttt attttcttta tcaggttttt catatcatgt atcattatac tataaaaaaa
     1321 accatatatc cccctttttt tcagtatttt gacgatttat attgttgcat ttttctgtaa
     1381 atgctatact ttaccgggag gtattttaca tgaaaaatct ttacattcgt gcggggatgg
     1441 ccccctttga aaaccttgac ccatatcatg tattgctaca caacagtata tccggcaatt
     1501 cgggaaatct cctctatgcc tacggccttt gcagggccat tatgacagag aatacaaata
     1561 tcgaagtcga ccattacaca acttcctcat cagacatgcc tgatatcaac aaaaaatatg
     1621 atgcatggat aatcccgttg gcagatgcgt tcaggaataa tttcacggaa gtcatggata
     1681 agctcacgaa atgtatcagg aaatcaagga tccctgttat ccttgtgggt gtgggtctta
     1741 gggctccttt ggaaacagat ctcagttcct cttttccctt tgacgatcat gtgaagaaat
     1801 ttgtaagcgc gatcctggac aaatccgcag ttatcggtgt acggggtgag accacgggca
     1861 gataccttac caaactgggc tttagggaag gctcccacta caaagtgatc ggttgtcctt
     1921 caatgtacgg ttttggagat acattgaaaa taagagaacc ccaaataaac aaaggatctc
     1981 tcatctcact gaattcatcc atattctccg aacagaagac tatcgatttc gtggagaggg
     2041 taagacagca gttccccaat cattacttcg tgcctcagca caggagagaa ctggcggaat
     2101 accacgggtt ttcatgtgta tatcaaaagg at
//
LOCUS       DAONHK010000166         2123 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_45550_length_2123_cov_3.429400, whole genome shotgun sequence.
ACCESSION   DAONHK010000166 DAONHK010000000
VERSION     DAONHK010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45550_length_2123_cov_3.429400"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="PLM92_05585"
     CDS             <1..528
                     /locus_tag="PLM92_05585"
                     /inference="COORDINATES: protein motif:HMM:NF025210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HPR24643.1"
                     /translation="LHIKKPFNNGGTKIMSRNLIVEPKVVSGVKVAGNIVIYTLRNIN
                     NNGRAVAELFDVLRDIDINIDMISQHNDGEGKCTVAFSCTGNQTEKMENALKSNPRLQ
                     GIEVSKQEDLSMVSIIGVGMASHSGVAGKVFNVLAEAGIPYYNITTSEISISVALDSE
                     NSSAAVVALGEAFRL"
     gene            533..1402
                     /gene="dapF"
                     /locus_tag="PLM92_05590"
     CDS             533..1402
                     /gene="dapF"
                     /locus_tag="PLM92_05590"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HPR24644.1"
                     /translation="MEIRFSKYHGCGNSFVIIKEQGIPAGDHSRLALEMCSESKGIGA
                     DGLIIARADPQFEMVFYNRDGSRAPMCGNGIRCFAVFCIKEGMTRKRKFKIKTMAGIM
                     EAEIKDRGNDLEAEINMGPPGPAASHGSMEKKEFLFYKKLKVSTGEIYVRTLFIGTLH
                     TVIWAEEYPMDELKSLCAEICSHPAFPEKTNVDIVRIIDRKTLEMFTYERGAGFTPAC
                     GTGACAAVAAGIAEGKIENKVDVFLPGGKLHIEQRKNKEIFMKGPAVFIAGGLYRRDT
                     NDTRLHGGADHTV"
     gene            1362..>2123
                     /gene="dapA"
                     /locus_tag="PLM92_05595"
     CDS             1362..>2123
                     /gene="dapA"
                     /locus_tag="PLM92_05595"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HPR24645.1"
                     /translation="MIRGSMVALITPYNDDGSVNLRRQAELIEWHIQNGTDSILILGT
                     TAETPALTAAEQEDIVRVSIDAAKGLKPVIVGSGSNNSLAAKEKSLKFQSMGADGLLV
                     ITPYYNKTNKAGMIHHFRAVADAVDIPVYIYNVPGRTGCAITYETLEELSKHPKIVGI
                     KEASGDISFVTKISRLINDDFNIYSGNDDMIVPLMSMGGEGVVSVLANILPAETHEMT
                     MAYINGETEKAAALQKKYLDFINALFIETNPMPVKE"
BASE COUNT          693 a          404 c          547 g          479 t
ORIGIN      
        1 cttcatataa agaaaccatt caataacgga ggaacaaaga taatgagccg taatctgata
       61 gtagagccca aagtggtgtc gggcgtaaaa gttgcaggca atatagtaat atacacatta
      121 agaaatataa ataacaacgg cagagctgtg gcagaacttt ttgatgtgct cagggacata
      181 gacataaata tagatatgat atctcagcat aatgacggtg aaggaaaatg tacggtggca
      241 ttcagctgca ccggaaacca gactgaaaaa atggaaaatg ccctgaaaag caacccgcgg
      301 cttcagggga tagaggtgtc aaaacaagag gatctttcca tggtatcaat aatcggcgtt
      361 ggtatggcca gccattcggg agtggccggc aaagtattca atgtacttgc tgaagccgga
      421 ataccttatt ataacatcac cacttcggaa atatccattt ccgttgccct ggattcggaa
      481 aacagcagtg ctgctgtagt cgctttggga gaagcgttca gactttaggg ctatggaaat
      541 aagattcagt aaatatcacg gctgcgggaa cagtttcgtg ataataaaag agcagggcat
      601 accggccggg gatcacagcc gccttgccct ggagatgtgt tctgaaagca aaggtatagg
      661 agccgacgga ttgatcatag caagagctga tcctcagttt gaaatggttt tttacaaccg
      721 ggacggaagc cgtgcaccta tgtgcggcaa cggaataagg tgttttgccg tattctgcat
      781 taaagaagga atgacccgga aaaggaaatt taaaataaag actatggccg ggatcatgga
      841 agcagaaata aaagacaggg gcaacgatct tgaggcggaa ataaatatgg gtcctcccgg
      901 acctgcagct tcccatggtt ccatggaaaa gaaagagttt cttttttata aaaaactgaa
      961 ggtctccaca ggtgagatat atgtaaggac attatttatt ggcacgcttc atacggttat
     1021 ctgggcggaa gaatatccca tggatgagct gaaaagcctt tgtgccgaga tttgttctca
     1081 tccggctttc cctgaaaaaa ccaatgtgga tatagtaagg ataatagaca gaaaaactct
     1141 tgaaatgttc acttatgaga gaggggcagg attcaccccg gcctgcggaa cgggcgcatg
     1201 cgctgcggtg gccgcgggaa tagcggaagg aaagatcgaa aacaaggtag atgtgtttct
     1261 gcccggaggc aagctgcata tcgaacaaag aaaaaacaaa gaaatattca tgaaaggccc
     1321 ggcagttttt attgccggcg gcctgtatag gagggataca aatgatacga ggctccatgg
     1381 tggcgctgat cacaccgtat aatgatgacg gcagtgtgaa tctcagacgc caggcagaac
     1441 ttatagaatg gcatatacaa aacggcacag acagtatact tattttgggg accacagcag
     1501 agaccccggc gctgactgcg gcagagcagg aggatatagt gagagtgtcc atagatgcgg
     1561 caaaggggct aaaacctgta atagtgggca gcggttcaaa caactcgctg gcagcaaagg
     1621 aaaaaagcct gaaattccag agcatggggg ctgacggact gctggtgata acaccttact
     1681 ataataaaac caacaaagcc ggaatgatac atcatttcag agctgtggca gatgcagtgg
     1741 atataccggt atatatttac aatgtgcccg gaaggaccgg ctgtgccata acctacgaaa
     1801 ctctggaaga gttgtcaaaa catccgaaaa tagtcgggat aaaagaagca agcggggata
     1861 tatcttttgt tacaaagata tccagattga taaacgatga tttcaatata tattcaggca
     1921 atgacgacat gatcgttcct cttatgtcaa tgggaggaga aggcgtggtg tctgttcttg
     1981 ccaatatact gccggcagaa acacatgaaa tgaccatggc ctatataaac ggtgaaacag
     2041 aaaaggctgc agcccttcaa aaaaaatatc ttgattttat aaatgcattg tttattgaaa
     2101 ctaatcccat gccggtaaaa gag
//
LOCUS       DAONHK010000167         2120 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_45644_length_2120_cov_4.078450, whole genome shotgun sequence.
ACCESSION   DAONHK010000167 DAONHK010000000
VERSION     DAONHK010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45644_length_2120_cov_4.078450"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..82)
                     /locus_tag="PLM92_05600"
     CDS             complement(<1..82)
                     /locus_tag="PLM92_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015523209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HPR24646.1"
                     /translation="MARIAGVDLPRDKRIDIGLTYIYGIGR"
     gene            complement(215..328)
                     /gene="rpmJ"
                     /locus_tag="PLM92_05605"
     CDS             complement(215..328)
                     /gene="rpmJ"
                     /locus_tag="PLM92_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HPR24647.1"
                     /translation="MKVRSSVKPICEKCKIIKRNGKVMVICDNPKHKQKQG"
     gene            complement(344..562)
                     /gene="infA"
                     /locus_tag="PLM92_05610"
     CDS             complement(344..562)
                     /gene="infA"
                     /locus_tag="PLM92_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HPR24648.1"
                     /translation="MAKKDVIEAEGVVVDAQPNAMFVVKLETGHEVLAHVSGKIRMNF
                     IRILPGDKVKVELSPYDLTRGRITWREK"
     gene            complement(577..1323)
                     /gene="map"
                     /locus_tag="PLM92_05615"
     CDS             complement(577..1323)
                     /gene="map"
                     /locus_tag="PLM92_05615"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003870250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HPR24649.1"
                     /translation="MIIIKSEHEIEIMRKSGKVTGTILKELEDFIKPGISTKSIDRHA
                     AEIIRENGMEASFKGYNGYPASVCTSINEEVVHGIPSGGRIIKEGDIVSVDVGCTYKG
                     YVTDAARTYPVGEISPEAQKIIDAAKDSFFEGLEFCREGYRLSDISNAIQTKAEGEGF
                     SVIRDLVGHGVGRKMHEEPQIPNYGIAGRGPRISRGMVFAIEPMIAQGTYEVFTGKDN
                     WTISTADGKLSAHYENTVVITGGAPELLTL"
     gene            complement(1328..1969)
                     /locus_tag="PLM92_05620"
     CDS             complement(1328..1969)
                     /locus_tag="PLM92_05620"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008881924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HPR24650.1"
                     /translation="MNLVLLGPPGAGKGTQAAMIIEKYGIPHISTGDIFRNNIKEGTP
                     LGKKAKEYMDKGELVPDSLVIEIATDRLDKEDCREGFLLDGFPRTVQQAKALDEHLAE
                     KGKQIDHVLDIRVEKDELIRRLTGRRVCRNCGATYHAVNIPPQKEGICDICGGELYQR
                     ADDNRETVENRIEVYKSQTSPLIDYYEKHDNIKHFDGSIGLEKLFAEIKELLG"
     gene            complement(1983..>2120)
                     /locus_tag="PLM92_05625"
     CDS             complement(1983..>2120)
                     /locus_tag="PLM92_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011026319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HPR24651.1"
                     /translation="IVGQITPLDVQFGGTSLLIAVGVALDTMKQLEQQMVMRNYQGFL
                     K"
BASE COUNT          460 a          554 c          376 g          730 t
ORIGIN      
        1 gtcttccaat accatagata taagttagac caatgtctat tctcttgtct ctaggtaagt
       61 caactccggc tatacgagcc atattctcct cctgttccca tcttccgtcg cgcaaccatc
      121 agctgtttca aggcttgggc gcaatataaa cgggatgttc ttcagcgcat tcactgcgca
      181 gttataactt aagcaccggc attgccggcg attatcaacc ttgtttctgc ttgtgcttgg
      241 gattgtcaca gataaccatg acttttccgt ttctctttat tatcttgcac ttttcgcaaa
      301 tgggctttac cgatgatctc actttcatct caactaaacc tccttatttt tctctccatg
      361 ttattctgcc tcgggtaagg tcataggggg acagttccac ttttactttg tctccgggaa
      421 ggatccttat gaaattcatt ctgatttttc ctgacacatg agccagtact tcatgtcctg
      481 tttccagttt tacgacgaac attgcattgg gctgcgcatc aaccactacg ccttctgctt
      541 caattacatc tttcttggcc ataaatccac ctctccttat aaggtaagca attccggcgc
      601 tccgccggtt atcacaacag tattctcata atgtgcggaa agttttccgt ccgccgttga
      661 aatagtccag ttatcctttc cggtgaacac ctcatatgtg ccctgggcta tcatcggctc
      721 tatggcgaat accattcctc ttgaaattct gggtcctctg cccgctattc cgtaatttgg
      781 tatctggggt tcctcgtgca tttttcttcc tacaccgtga cccacaagat cccttataac
      841 tgaaaaacct tctccttcgg ccttagtctg tatggcgttt gatatatcgg aaagtctgta
      901 tccttctctg caaaactcca gaccttcgaa aaaactgtct tttgcggcat caattatttt
      961 ctgagcttca gggcttattt cgcccaccgg atatgttctt gccgcatctg taacatatcc
     1021 cttatatgta cagcccacat ctacacttac tatgtcacct tcttttatga tccgtcctcc
     1081 tgagggtatt ccgtgaacga cctcttcatt tatggatgtg caaacgctgg ccggatatcc
     1141 gttatagcct ttgaatgaag cttccatccc gttttcacga atgatttccg ctgcatgtct
     1201 gtcaatagac ttggtggata tcccgggctt tatgaagtct tccagttctt tgagtatagt
     1261 gccggtcact ttgcccgatt tgcgcattat ttctatttca tgctctgatt ttattattat
     1321 cattctatta tcccaaaagt tcttttattt cggcaaaaag tttttccaat ccgatggatc
     1381 cgtcaaaatg ttttatattg tcatgttttt cataataatc tatcagaggt gaagtctgcg
     1441 acttataaac ttcgatcctg ttttctacag tttccctgtt gtcatctgcc ctttggtaaa
     1501 gctctccacc gcagatatca catattccct ccttttgggg aggtatattt accgcatgat
     1561 aagttgctcc gcagttcctg catactcttc ttcccgtaag tcttcttatg agctcgtcct
     1621 tctctaccct tatgtcaagt acatgatcta tctgtttgcc tttttccgcc agatgctcat
     1681 ccagtgcctt tgcctgttga acagttctcg ggaatccgtc aagaagaaaa ccttcacggc
     1741 aatcctcctt atccagcctg tctgttgcta tttctataac cagactgtca ggaacaagtt
     1801 ctcctttgtc catgtactct ttagcctttt taccaagggg agtcccttct tttatattgt
     1861 ttctgaaaat atctcccgtt gatatatgag gtattccgta tttttctatt atcattgcag
     1921 cctgggtgcc tttaccggct cccggggggc ccaataaaac cagattcatt atgtcacctc
     1981 cgtcacttaa ggaatccctg ataatttctc atgaccatct gctgctccag ctgtttcatt
     2041 gtatccaagg caacgcccac tgctatgagc agtgaagtcc ctccgaactg tacgtcaagc
     2101 ggagttatct gtcctactat
//
LOCUS       DAONHK010000168         2119 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_45674_length_2119_cov_4.176357, whole genome shotgun sequence.
ACCESSION   DAONHK010000168 DAONHK010000000
VERSION     DAONHK010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45674_length_2119_cov_4.176357"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..752
                     /locus_tag="PLM92_05630"
     CDS             <1..752
                     /locus_tag="PLM92_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HPR24652.1"
                     /translation="LGVGAGNVQCIIDKGYDYKQAVPKIIAGRTFDYGIICSGEQSFI
                     CPEEDYENILREFEKNGAYIVKDEEEAERLRNVLFEEGKPNRHSVGQSPQKIAEMAGI
                     SLPEGTRVIVAQAKGTAGSDLFGKEKMAPVIAAYKYRTFEEGVEIAEANLSVEGKGHS
                     VAIHSDNIRHIEYAGEKLSVSRFVVNQTCASTAGGSFYNGLAPTNTLGCGSWGHNSIS
                     ENLDFKHLMNVSRIAYFMKDNHVPDREELWG"
     gene            753..1985
                     /locus_tag="PLM92_05635"
     CDS             753..1985
                     /locus_tag="PLM92_05635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HPR24653.1"
                     /translation="MRSDKYKKRNAAKAAKEARKSKSGRRKYSKEDDDFFAGFDELDN
                     DEPIKTKKSSKKARRRGGKTPTKRKKTVKTVIICLVLLLLALSAGAYIFMTHKLSKMD
                     YVDTGSVEWCIDDRVAEELKDYRNFAVLGVDARDMESYDGSNNDAIIIVSLNKKTDKI
                     KMISVMRDSYLLVDKKQQDDLYLTKITHAHAIGGGVWTCRALNRNLDLNIEEFAIFNW
                     KAVADLVDAVGGVTVEVEGNEINDFNHYGQETAEKVGGTFSAISYPGEYDLDGIQAAT
                     YCRIRKTSGGDSQRAERMKKVMTAIFKKAKTMDLSTLNNIADEMLPEIRTNIKSGSML
                     KLMAGIKGYELNGNEGFPYKYAGGMYHGGWYAVPQTLKSNVKELHKKAFKQKKYRPTQ
                     TVREINEMIINDTGVTLP"
BASE COUNT          725 a          399 c          548 g          447 t
ORIGIN      
        1 cccttggagt gggagccgga aacgtgcagt gcataataga caagggctat gattacaaac
       61 aagctgtacc taagataata gccggaagga cctttgacta cggcataata tgttccggag
      121 aacagtcatt catatgcccc gaggaagact atgagaatat actcagggaa ttcgaaaaaa
      181 acggagcata tatagtaaag gatgaagaag aggctgaaag actcagaaac gttctgtttg
      241 aggaaggaaa acccaacaga cactcagtgg gccagtctcc ccaaaaaatc gctgaaatgg
      301 cggggatatc ccttcctgaa ggcacccgtg tgatagtggc acaagcaaaa gggactgccg
      361 gatcagatct tttcggtaaa gaaaagatgg caccggtgat agccgcatat aaatacagga
      421 cctttgagga aggggtcgaa atagccgaag caaatctttc ggtggaaggc aaaggccaca
      481 gtgttgccat ccattcggac aatatcaggc atatagagta tgccggcgag aaactgtcgg
      541 tttccagatt cgtggtcaat cagacatgcg ccagcaccgc cggaggcagc ttctataatg
      601 gtcttgctcc cacaaatacg ctgggatgcg gatcctgggg acataatagt atttcggaaa
      661 acctggactt caaacatctt atgaatgttt ccaggatagc atatttcatg aaggataatc
      721 atgtacctga ccgggaggaa ttgtggggat aaatgagaag cgataaatac aaaaaaagaa
      781 atgccgccaa ggcagcaaag gaagcaagga agtcaaagag cggccggaga aaatattcca
      841 aagaagacga tgattttttt gccggatttg acgaattaga taacgatgaa cctataaaaa
      901 caaaaaaaag ctcaaaaaaa gcaagacgaa gggggggcaa gacccccaca aaacgtaaaa
      961 aaaccgttaa aacagttatt atttgtcttg tgctgctgct tcttgccctt tcagccggtg
     1021 catatatttt catgacacat aagcttagca agatggatta tgtggatacg ggcagtgtgg
     1081 aatggtgcat agatgacaga gtggcagaag aactgaagga ttacaggaat tttgctgttt
     1141 tgggggtcga tgccagggac atggaaagtt atgacggatc aaacaatgat gcgataataa
     1201 tagtgagcct gaacaagaag accgacaaga taaagatgat atccgtcatg agggacagct
     1261 atctgctggt ggataaaaaa cagcaggacg atctttacct gacaaagatc acccatgccc
     1321 atgccatagg cggaggagtc tggacatgca gggcactgaa caggaatctg gatctcaata
     1381 tagaagaatt cgccatattc aactggaaag cggtggctga tctggtggac gccgtgggcg
     1441 gtgtaacagt tgaggtggaa ggaaacgaaa taaacgattt caatcattac ggtcaggaga
     1501 ccgccgaaaa ggtgggcgga acattttccg ccataagcta tcccggggaa tatgatctgg
     1561 acgggatcca ggcggccacc tactgcagga taagaaaaac ctccggaggc gacagtcaga
     1621 gagcggaaag aatgaaaaaa gtcatgaccg ccattttcaa aaaggccaag accatggatc
     1681 tgagcaccct gaacaacatt gccgatgaaa tgctgcctga aataagaacc aacatcaaaa
     1741 gcggatcaat gctgaaactg atggcgggaa taaagggata cgagcttaac gggaatgagg
     1801 gcttcccgta taaatatgcc ggagggatgt atcacggcgg atggtatgca gttccccaga
     1861 ctctgaagtc caatgtgaag gaacttcaca agaaggcttt caaacagaaa aagtacagac
     1921 caacgcagac ggtcagggaa ataaatgaaa tgataataaa tgatacgggc gtgactttac
     1981 cataaaaaat gagaaaatat atggatatca gaaacatgtt tctgatatcc atatttattt
     2041 ggggataata ttgttttttc atattgacaa gcattgatat tgcagtatac tacagtaagt
     2101 acttactgta ggaggaaat
//
LOCUS       DAONHK010000169         2108 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_46026_length_2108_cov_3.940088, whole genome shotgun sequence.
ACCESSION   DAONHK010000169 DAONHK010000000
VERSION     DAONHK010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46026_length_2108_cov_3.940088"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..605
                     /locus_tag="PLM92_05640"
     CDS             <1..605
                     /locus_tag="PLM92_05640"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HPR24654.1"
                     /translation="EIRSDKGETIKKFESEVVRQVVSKETADEMCLIMESVVEEGGAG
                     TAKIDGYRVGGKTGTANKVVNGEYSEAVCSSFIGMAPMDDPKIAVLFIVDNPKGQIYG
                     SATAAPGARQVLENALRYMKVEPDYTAAERQANSKKTVTVPNVTGESYSEAIGILGGA
                     DLKHKVSPDMGDGADFIVKDQYPKAGDEIEKTGTVYLYRK"
     gene            647..2029
                     /gene="murF"
                     /locus_tag="PLM92_05645"
     CDS             647..2029
                     /gene="murF"
                     /locus_tag="PLM92_05645"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HPR24655.1"
                     /translation="MRKFTFREIEKAVGGKQISGSPDTVVLGVSKDSREAYSGSLFFP
                     LKGEKYDAHSFLPQVIQRGCRTFLISDEEILNVLKDKEDLDIIKVKDTTAALQDMAGY
                     YLKQMSLKKIAVTGSVGKTSTKDMIHCVCSSKYKTGKTKGNQNNHIGLPLGILSLDED
                     TEAAVLEMGMDRPGEIDFLADLVRPDIGVITNIGISHKENLGSREGIFRAKMELVNYF
                     GRENVLIINSGKDFLLKSNIEGEYRLLSAGTEKDDDFVVSDIWDKGEKGTEFFIKYRD
                     EKQFFRLSVPGRHNAFNAALAAACGMELGIDLKGSSEALLSLEITEGRLTVKEKNGIK
                     VIDDTYNASPDSMKAGIDTLNAVSSKRTIAILGDMFELGDDADIHHRKIGEYAKNSGV
                     DMVVAVGEMARHIAAGAAEKGHYYSNKEALKKDINDIVKKGDAVLVKASRGMEMEEIV
                     KIIIDKQEKK"
BASE COUNT          737 a          353 c          551 g          467 t
ORIGIN      
        1 aagagataag atccgacaaa ggcgaaacaa taaaaaaatt cgaaagcgaa gtggtaagac
       61 aggtggtatc caaggaaacc gccgatgaaa tgtgtcttat aatggagtct gtagttgaag
      121 aaggcggtgc cggtacagcc aagatagacg gatatagagt aggcggaaaa acagggacag
      181 ccaataaggt cgtaaatggc gagtacagcg aggcggtatg ttcttctttt ataggtatgg
      241 cgcctatgga tgatccaaag atagccgtac ttttcatagt ggataatccg aaagggcaga
      301 tatacggaag tgccacggca gctccgggag ccaggcaggt gcttgaaaat gccctgagat
      361 atatgaaggt cgaaccggat tatacagcag cggaaagaca ggcaaacagt aaaaaaacag
      421 ttacagtccc caatgtcacc ggggaaagct acagtgaagc catagggata ctgggaggag
      481 cggatcttaa gcacaaggtg tcaccggata tgggtgacgg agccgatttc atagtgaaag
      541 atcagtaccc gaaagcaggg gacgaaatag aaaaaacagg cacagtatat ctgtacagaa
      601 aatagcgatt gatgtataat atactgatta tgccggagaa atatttatga gaaaattcac
      661 tttcagagaa attgaaaagg ctgttggcgg aaaacagata tcaggatctc ctgatactgt
      721 cgttcttggg gtctcaaagg attcgcggga ggcttacagc ggttcattgt ttttccctct
      781 gaaaggagaa aaatatgatg cccacagctt tttgcctcag gttatacaaa ggggatgcag
      841 gacttttctg atatccgacg aagagatact gaacgtcctg aaggacaaag aagaccttga
      901 tataataaaa gtaaaagaca ccactgccgc actgcaggat atggcaggtt attatctgaa
      961 acagatgtca ttgaaaaaaa tagcagttac cggaagtgta ggcaagacat ccacaaaaga
     1021 catgatacat tgtgtatgca gcagcaaata taagacggga aaaacaaagg gcaatcagaa
     1081 caaccatatc ggtctgcctt tgggaatact cagtttagat gaagatacag aagcggcggt
     1141 tcttgagatg ggaatggaca gaccgggaga aatagatttt ctggcagacc ttgtaagacc
     1201 ggacatagga gtaataacaa acataggcat ttcccacaag gaaaacctgg gcagccgtga
     1261 aggtatattt cgtgccaaaa tggagcttgt caattatttc gggagagaaa acgtgcttat
     1321 aataaacagc ggaaaggatt ttcttctgaa aagtaatata gaaggtgagt acaggctttt
     1381 aagtgcggga acagaaaagg atgatgattt tgttgtttct gacatatggg acaaaggaga
     1441 aaaagggaca gagtttttca taaaatacag agatgaaaaa caatttttca gactttcggt
     1501 gccgggccgg cataacgctt ttaatgctgc tttggctgcg gcctgcggga tggagcttgg
     1561 aatagaccta aagggatcat cagaagcgct gttatccctt gaaatcacgg agggaagact
     1621 gaccgtaaaa gaaaaaaacg gcataaaagt aatagatgac acatataatg caagtccgga
     1681 ttcaatgaag gccggcatag acactcttaa tgctgtctca tcaaaaagga caatagccat
     1741 actgggagac atgtttgagc tgggcgatga tgctgatatc catcacagga agataggaga
     1801 atatgcgaaa aactccggag tggatatggt ggtagccgta ggagaaatgg cccggcatat
     1861 agcagcgggt gcagcagaaa aaggacatta ttacagtaat aaggaagcac tcaaaaaaga
     1921 cataaacgac atagtaaaaa aaggagatgc tgttcttgtg aaagcttcca gaggcatgga
     1981 aatggaagaa atagtaaaaa taattatcga taaacaggag aaaaaatgag ttacggaata
     2041 attgttatag taggtctttt gggactcacc tcgggtatac tgcttacatc gcttttgata
     2101 cctgttct
//
LOCUS       DAONHK010000170         2083 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_46868_length_2083_cov_4.598619, whole genome shotgun sequence.
ACCESSION   DAONHK010000170 DAONHK010000000
VERSION     DAONHK010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46868_length_2083_cov_4.598619"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="PLM92_05650"
     CDS             <1..331
                     /locus_tag="PLM92_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012093792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HPR24656.1"
                     /translation="TSPNTLIEGYYAAEAKSFTKGTVSLIRVSKKFIEKGDRVLIIDD
                     FLAHGQASLAMTDIIKQAGAHVAGIGIVIEKLFQGGGEKLREAGHRVESLASISKIND
                     GIITFAK"
     gene            517..1620
                     /locus_tag="PLM92_05655"
     CDS             517..1620
                     /locus_tag="PLM92_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009528157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HPR24657.1"
                     /translation="MKKVFAVAVALIMVLTLSFGLVGCGESEEESETLKVGFLYLGTA
                     KDGGFTQAQDEGRAAMEKHFDGAVETMAVEEVDEDKDAVITAGKNLLDQGCQVIIAGS
                     FGFMDGMEELANEYPDQYFLHFSGNKSNDTNFDNFFGAMEEPRYLAGIVAGMTTETNK
                     VGYVAAFPYTEVNIGINSFALGVQSVNPDAEVKVVYINTWYDPTKEKEAAEALLDQGC
                     DVLEQHCDTVGPTEAARDAEAHSIGYNLDIGKDYPETFMTAPIWHYDAYYIQVVQDIM
                     DGKFKPSQYYGNMADGFVDLAPLADFVSDDIKAKVEEVKAEIVAGDFAPLSGEIQFAN
                     GEYWCKEGQTLTREEIWLDELPLVKGVTSSEYE"
     gene            1639..>2083
                     /locus_tag="PLM92_05660"
     CDS             1639..>2083
                     /locus_tag="PLM92_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004623092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPR24658.1"
                     /translation="MQNIAIQMEKITKSFGALKANEDVDLIVKKGEVHALLGENGAGK
                     STLMNMLSGIYVPDSGSIFVEGQRVTFTSPKDSIGLGIGMIHQHFKLVDILTAKDNVV
                     MGQKAKKDSRDKDLSKEIRDLSDMYGLDIDPDKKVYRMSVGEKQTL"
BASE COUNT          681 a          372 c          525 g          505 t
ORIGIN      
        1 gacttcgccg aataccttga tagaagggta ttatgcagcg gaagcaaagt cttttaccaa
       61 aggcactgtt tccctgatca gagtgtcaaa gaaattcatc gaaaagggag acagggtcct
      121 tataatcgat gattttctgg ctcacgggca ggcatccctg gcaatgactg atataataaa
      181 acaggcggga gcccatgtcg caggtatcgg aatagtaata gaaaagctgt tccagggtgg
      241 aggagagaag cttagagagg cgggacacag ggtagaatcc ctggcttcca taagcaaaat
      301 aaacgatggc atcataacct ttgcgaaatg aacggtatat agcgatatta tatataatgt
      361 tcggattata acgaacatta tatttttttt ggtatcaaca atatgctttt ttacgaaaaa
      421 gacttcctgt tggtcagggt aaagactata atgaatttat caaaaacagt tcagaaagag
      481 tttttgcttt ttatttttag gaggagaagg agaaaaatga aaaaagtatt tgcagttgcg
      541 gtagcgctaa taatggtgct gactctgtca ttcggtcttg tgggctgcgg tgaaagtgaa
      601 gaggaaagtg aaacactgaa agtgggcttc ctctatttag gcacagcaaa agacggcgga
      661 ttcactcagg ctcaggatga gggcagagca gccatggaaa aacatttcga cggtgcagtt
      721 gagactatgg ccgttgaaga ggttgatgaa gacaaagatg cagttataac agcaggcaaa
      781 aatctgctgg atcagggatg ccaggtgata atagccggaa gcttcggttt catggacggc
      841 atggaagaac tggccaatga atatccggat cagtatttcc tccatttctc aggcaacaaa
      901 tccaatgaca ccaattttga caatttcttt ggagccatgg aagagcccag atatctggca
      961 ggtatagttg caggcatgac cacagaaaca aacaaagtcg gatatgttgc ggcattccca
     1021 tacacagaag taaatatcgg aataaattca tttgctctgg gagttcagtc tgtaaatccc
     1081 gatgcagaag taaaagttgt atatatcaat acatggtatg atccgactaa agaaaaagaa
     1141 gcagctgaag ctctgcttga tcagggatgc gacgttcttg aacagcactg cgatacagtg
     1201 ggacccacag aagcagcaag agatgcagag gctcattcaa tcggatacaa tcttgatata
     1261 ggcaaggatt accccgaaac atttatgaca gctcctatct ggcactatga tgcatattac
     1321 attcaggtag tacaggacat aatggacggc aaattcaaac cgagccagta ttatggaaac
     1381 atggctgacg gattcgttga cctggctcct ctggctgatt tcgtttcaga cgatataaag
     1441 gcaaaagttg aagaagtaaa agcagaaata gttgcaggcg attttgctcc tctttcgggc
     1501 gagatacagt ttgcaaacgg agaatactgg tgcaaggaag gacagacact gacaagagaa
     1561 gaaatatggc ttgacgagct tcctcttgtg aaaggcgtta cttcatcgga atatgaataa
     1621 ggattcggag gactgacagt gcagaatatt gcaattcaaa tggagaagat caccaaatcc
     1681 tttggagcac tgaaagccaa tgaagatgta gatttaattg tgaaaaaagg cgaagtacat
     1741 gcccttctgg gggagaacgg agccgggaaa tcaacattga tgaatatgct gtcggggata
     1801 tatgtccccg acagcggttc tatctttgtt gaaggtcaaa gagttacttt tacatctcca
     1861 aaggattcca ttgggctggg gatcggaatg atccaccagc attttaaatt ggtggatatc
     1921 ctgaccgcaa aagataatgt ggtaatggga cagaaggcaa aaaaagacag cagagacaag
     1981 gatctttcca aagaaataag ggatctgtcc gacatgtacg gtcttgatat agatccggac
     2041 aaaaaagtat accgcatgtc agtgggagaa aaacagaccc ttg
//
LOCUS       DAONHK010000171         1983 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_50723_length_1983_cov_3.344917, whole genome shotgun sequence.
ACCESSION   DAONHK010000171 DAONHK010000000
VERSION     DAONHK010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1983
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50723_length_1983_cov_3.344917"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1189)
                     /locus_tag="PLM92_05665"
     CDS             complement(<1..1189)
                     /locus_tag="PLM92_05665"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003450306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HPR24659.1"
                     /translation="MSKEVSMEKLVALAKNRGFVYPGSEIYGGLANSWDYGPLGVEFK
                     NNLKKAWWKKFVQESKYNVGIDAAILMNPRVWEASGHVAGFADPLVDCKNCKARFRAD
                     KLIEDHMEAQGVTGITVDGWPREKLEEYIKEKNLSCPDCGKSDFTGIRQFNLMFRTFQ
                     GVTEDSGSEIYLRPETAQGIFVNFKNVQRTSRKKIPFGIAQIGKSFRNEITPGNFIFR
                     IREFEQMELEFFCKPGTDLEWFDYWKEYCLNFLLSLGMKKENIKLRDHEKEELSHYSN
                     ATTDIEYLFPFGWGELWGIADRTDFDLNQHIGHSGQEMNYMDPETNEKYIPYCIEPSL
                     GADRIALAFLCDAYDEETITDDKGKEDMRVVMRFHPAIAPYKAAVLPLAKKLSYLAEP
                     IKEE"
     gene            complement(1190..1870)
                     /locus_tag="PLM92_05670"
     CDS             complement(1190..1870)
                     /locus_tag="PLM92_05670"
                     /inference="COORDINATES: protein motif:HMM:NF025601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HPR24660.1"
                     /translation="MEITLEKIELVKDRTGVSYKEAKEALEAAEGSVVDAIINIEETL
                     DEDSKKTFGVKGAAIVDSIKELVKKGNVSKIVVKKQDGDQVVNIPVNVGIVGIVIAPW
                     GMLVGAIAAFGFKCVIEIVKNDGTIIDVSDRANEAMNKAKEKGVEFADAAKEKGGDVY
                     EKVKNSDVYEKVKSSDAYEKVKDAASDAINKTSDAIKNKKEKAAEDFADTFAEDIEDE
                     DDKKGDKE"
BASE COUNT          438 a          473 c          334 g          738 t
ORIGIN      
        1 gttcttcttt tattggctca gccagatatg acagtttttt tgccagcggc agtactgctg
       61 ctttgtatgg tgctatggca ggatgaaaac gcattacgac cctcatatct tcttttcctt
      121 tatcatcggt aatggtttct tcatcgtagg catcacaaag gaaagccagt gctatcctgt
      181 cggctccaag agaaggttct atacaatagg gtatatactt ttcattggtt tcgggatcca
      241 tataattcat ttcctgtccc gaatgaccga tatgctgatt gaggtcaaaa tctgttctgt
      301 cggctatgcc ccacagttct ccccaaccga aaggaaacag atattcaatg tctgtggtgg
      361 cattgctgta atgggacagt tcttcttttt cgtgatctct cagctttatg ttttcttttt
      421 tcatccccag tgataaaagg aaattcagac agtattcctt ccagtaatca aaccattcaa
      481 ggtctgtccc cggcttgcag aaaaactcaa gttccatctg ttcgaactca cgtatcctga
      541 atatgaaatt accgggagtt atttcgtttc ggaaagattt ccctatctgg gctattccaa
      601 aaggtatttt ttttctggaa gttctttgta cattcttgaa atttacaaat atcccctgag
      661 cagtttcagg tcttaaatat atttccgatc cggaatcttc tgttactccc tgaaaagtcc
      721 tgaacatcaa attgaactgt cttatgccgg taaaatcgct tttaccgcag tccggacagc
      781 tgagattctt ttcttttatg tattcttcaa gtttttctct gggccagccg tctacggtta
      841 tgccggtcac tccttgagct tccatgtggt cttcaataag cttatctgct ctgaacctgg
      901 ctttgcagtt tttgcagtcc accaaggggt ctgcaaatcc ggcaacatga cctgatgctt
      961 cccataccct gggattcata agtatggctg catctatacc gacattatat tttgattcct
     1021 gcacaaattt tttccaccag gcttttttta gattgttttt gaactccact cccaaaggac
     1081 cgtagtccca tgagttagcc agtcctccgt atatctctga accgggatat acaaatcctc
     1141 tgtttttggc cagagcgacc agtttctcca tagatacttc tttgctcatc tattctttat
     1201 cgcctttctt gtcatcttca tcttctatgt cttctgcaaa tgtatcggca aaatcttcag
     1261 cagccttttc ttttttgttt tttattgcgt cggaagtttt gtttattgca tcagatgcag
     1321 catcttttac tttctcatag gcatcactgc ttttgacttt ttcgtaaaca tcgctgttct
     1381 ttactttttc atatacgtcg ccgccttttt cttttgcagc atctgcaaat tccacaccct
     1441 tttctttggc cttgttcatg gcttcgttgg ctctgtcgct tacatctatt attgttccgt
     1501 cgtttttgac tatttcaata acacacttga atccaaatgc agctattgct ccaaccagca
     1561 ttccccaagg tgctattact atacccacta tacccacgtt tacaggtata ttaacgactt
     1621 gatctccatc ctgttttttt acgactattt ttgaaacgtt gcctttcttg accagttcct
     1681 ttatggaatc tactatggcg gcacccttta ctccgaaggt ttttttgctg tcttcgtcaa
     1741 gggtctcctc tatgttgata atggcatcaa caacacttcc ttcagcagct tcaagcgctt
     1801 ctttcgcttc tttgtaactt actcctgtcc tgtcttttac aagctcgatt ttttccaatg
     1861 tgatttccat ggtatacctc ctcaattatc agttaaatat ttttcacttt tcattttgtc
     1921 tatttccaga tgatatgaaa tatattctcc caggatcttt tttatttttt cgggtctgtc
     1981 tcc
//
LOCUS       DAONHK010000172         1956 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_51833_length_1956_cov_3.534456, whole genome shotgun sequence.
ACCESSION   DAONHK010000172 DAONHK010000000
VERSION     DAONHK010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1956)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1956)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1956
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51833_length_1956_cov_3.534456"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            114..314
                     /gene="rpmE"
                     /locus_tag="PLM92_05675"
     CDS             114..314
                     /gene="rpmE"
                     /locus_tag="PLM92_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005998913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HPR24661.1"
                     /translation="MKEGIHPKYQECKVTCACGNEFVTKSIEKEIRLDVCSACHPFFT
                     GQQKFINRGGRVEKFKNKYNMD"
     gene            404..1471
                     /gene="prfA"
                     /locus_tag="PLM92_05680"
     CDS             404..1471
                     /gene="prfA"
                     /locus_tag="PLM92_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005998885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HPR24662.1"
                     /translation="MFDKLDFISDKYDELSAKVSDPEIIANQALWQKYAKEMGEMEPI
                     VKKYQEYKKIKGDLKGAKEMLDEGGMDEELKEMAKAELAELEEKTKPLEEELKVLLLP
                     KDPNDDKNVILEIRAGTGGEEAALFGMDLLRMYKMYAENRGWKSEIIEINDTDIGGIK
                     EVVVLIKGKGAYSRLKYESGAHRVQRVPETESSGRVHTSAATVAVLPEVEGVEIEVNP
                     NDVRVDVYRASGNGGQCVNTTDSAVRLTHEPTGLVVTCQDEKSQIKNKDKAFKVLKSR
                     LYDLKMQEQNKEIADKRKSQVGSGDRSERIRTYNFPQGRVSDHRTGTTIYKLESFLNG
                     NMDEIIDGLITHDQAEKMRAF"
     gene            1595..>1956
                     /locus_tag="PLM92_05685"
     CDS             1595..>1956
                     /locus_tag="PLM92_05685"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HPR24663.1"
                     /translation="MKTQILESTSENIKIAAEIITKGGLVAFPTETVYGLGADAMDQE
                     AVKKIYKAKGRPSDNPTIVHIGRASDIGQLTRMLSPGIVNLIDNFWPGPLTLVVNKKP
                     EVPDVTTGGLGTVAVRMP"
BASE COUNT          724 a          338 c          492 g          402 t
ORIGIN      
        1 atcacaaaaa ataaaaaggt tgataccttg aaacaactgt ggtatagtaa ataagctgtg
       61 tatagaaagc aaattatgca aaggggaacc tgagcaggaa agaggtgata agcatgaagg
      121 aaggaatcca tcctaaatat caggaatgta aagtaacttg cgcatgcggt aacgagttcg
      181 ttacaaaatc gatagaaaaa gaaataagac ttgacgtttg ctcagcatgc cacccgttct
      241 ttacaggaca gcagaagttc ataaacagag gcggtcgtgt cgagaagttt aagaacaagt
      301 acaatatgga ttaattatta aaaccggaat tgattttccg gtttttttat aggcctgatt
      361 atgatataat aatcatttga aaatagacgc aagggagata aaaatgttcg ataaactgga
      421 tttcatttca gataaatatg atgaactttc ggcaaaggtc tcagaccctg aaatcattgc
      481 aaatcaggca ctttggcaga agtatgccaa agagatggga gagatggagc ccatagtcaa
      541 aaaatatcag gagtacaaaa agatcaaggg agatcttaag ggtgccaaag aaatgcttga
      601 tgaaggcggc atggatgaag agcttaagga gatggcaaaa gcagaacttg ccgagcttga
      661 agaaaaaaca aagcctttgg aagaagagtt gaaggtactt ttgctgccaa aggatcctaa
      721 cgacgacaaa aacgttattc tggagatccg tgcggggacc ggcggagaag aggctgcgct
      781 ttttggcatg gaccttcttc gcatgtataa gatgtatgcc gaaaaccgag gctggaaatc
      841 cgaaataata gagatcaacg acaccgatat aggcgggata aaggaagtgg ttgtgctcat
      901 caaaggaaag ggtgcatatt ccaggctgaa atatgaaagt ggggcacaca gagtgcaaag
      961 ggtccccgaa actgaatcca gcggcagggt acacacttct gctgccactg tagctgtgct
     1021 tccggaagtg gaaggggttg aaatcgaagt aaacccgaat gatgtaagag tggatgtcta
     1081 cagggcatca ggaaacggcg ggcaatgtgt aaatacaacg gattcggcgg taaggctcac
     1141 gcatgagcct acaggtcttg tagtgacatg tcaggacgag aagtcccaga taaaaaataa
     1201 agacaaggct tttaaagtat tgaaatcgag gctttatgat ctgaaaatgc aagagcagaa
     1261 caaagagata gcagacaaga gaaaaagcca ggtgggaagt ggagacagaa gcgaaagaat
     1321 aagaacctac aattttccgc agggcagagt ttcagatcac agaacaggaa ccaccatata
     1381 taaactggaa agttttttga acggaaatat ggatgaaata atagacggcc tgataactca
     1441 tgatcaggca gaaaaaatga gagcctttta gaaacgcaga tagtagaaga gatcagcaaa
     1501 gcgaagcatt agcacaaatc agcaaaacaa ataaatagca caagcagcac tgatcaccaa
     1561 caaaaaaaca agacaggaaa gcaaaagaaa gaatatgaaa acacaaatcc ttgaaagcac
     1621 atctgaaaat ataaaaatag cagccgagat aatcacaaaa ggcggtctgg ttgcattccc
     1681 cacagaaaca gtatatggac tgggtgcgga tgcaatggat caggaagctg taaaaaaaat
     1741 ctataaggcg aagggaagac ccagcgataa cccaacaata gttcatattg gaagggccag
     1801 tgacataggg cagcttaccc gcatgctttc tcccggaata gtcaacctta ttgataattt
     1861 ctggccgggc ccattgactt tggtcgtaaa caaaaaaccg gaagttcccg atgttaccac
     1921 agggggactt ggtactgtgg cagtaaggat gccgga
//
LOCUS       DAONHK010000173         1953 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_51968_length_1953_cov_3.039515, whole genome shotgun sequence.
ACCESSION   DAONHK010000173 DAONHK010000000
VERSION     DAONHK010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1953
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51968_length_1953_cov_3.039515"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      complement(<1..47)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(176..838)
                     /locus_tag="PLM92_05690"
     CDS             complement(176..838)
                     /locus_tag="PLM92_05690"
                     /inference="COORDINATES: protein motif:HMM:NF012753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HPR24664.1"
                     /translation="MDPKTNKLIFIYVTVSRGTGSKILQEAKRSGLSGGTVFLGINIF
                     KSPILDALSLNYEKNEIIFIVAEKNEGILFLEKISKEFKFHKKNHGTAFVTDVDFVCG
                     SSCLSCSGFDKNKGDEISMYQCVYIIVERGKGEDAASAAIDGGAGGATIVFARGSGAH
                     EKSRLFNMDIEPEKEIVMVVVNSEITEKVVSNVRREMEIDKPGNGIIFIQNVSQVYGA
                     LV"
     gene            complement(819..>1953)
                     /locus_tag="PLM92_05695"
     CDS             complement(819..>1953)
                     /locus_tag="PLM92_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005813698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1538 family protein"
                     /protein_id="HPR24665.1"
                     /translation="IVSFGIAVLLSAGLLRIVYNFGLHKMLTILYGIIFAIAIFASPE
                     FIAISFDASGATTGALTVPFILALAVGVSALKRDSQASEKDSFGLVAIVSTGPIISVM
                     IMSLIIGAEDISGSVDIVAGDDGSIFHPFMYKMPVMAYEVFFALLPILLVFLILRKRA
                     FKLGKQQTKDILLGIVYSFVGLVLLLTGVNAGFMEVGTIVGFQIASLENDFLLIGTGF
                     LLGLLTILAEPAVYVLTDQIENVTSGYVKRKTVMGALAIGVGTSVALSMIRILVPDLD
                     LWHYLLPGYIIAVILTYIVPKLFVGIAFDSGGVASGPMTATFILAFAQGAAEGIPGAD
                     VMLDGFGIIAMVALTPLITLQILGIIYKIKTRKGGSENGSENQ"
BASE COUNT          524 a          492 c          360 g          577 t
ORIGIN      
        1 ctttagcttt gcgcccccgc ctttcggcgg atttgcctta tttatccatc ttttagaagt
       61 ataaaacaaa tctttctctt tgtaaaggtt ttttgagcac agtcttataa aatggcatcc
      121 ttgccatttt tttcggcagt tttctagccg gtcccttgct cttctaattt agatctcata
      181 ccagagcgcc atagacctga ctcacgtttt gaataaatat aatgccatta ccgggcttgt
      241 ctatttccat ttccctgcgt acatttgaaa cgactttttc tgttatttcg ctgttgacta
      301 ccaccatcac tatttctttt tcgggttcta tgtccatgtt gaaaagcctg cttttttcat
      361 gagctcctga ccccctggca aaaactatgg ttgctcctcc ggcaccgccg tctatggctg
      421 cagaagccgc atcttcccct ttgcctcttt ctacaataat ataaacacac tgatacatgg
      481 atatctcatc tcctttgttt ttatcaaaac cgctgcagct gagacagctt gacccgcata
      541 cgaaatccac atctgtcaca aatgcagtgc catggttttt tttgtgaaat ttgaattctt
      601 tgcttatttt ctcaaggaac aggatccctt cgttcttttc agcaacaata aatatgatct
      661 cattcttttc ataattcagt gacagtgcat caagtattgg gcttttgaat atattgatcc
      721 ccaggaaaac agtccctccc gaaagaccgc ttcttttggc ttcctgaagt attttgcttc
      781 ccgttcctct gctgaccgtc acataaatga atatgagttt attggttttc ggatccattt
      841 tccgatcctc cttttcgggt ttttatttta tatataattc caagtatctg cagcgttatc
      901 aggggagtca atgccaccat ggctattatt ccaaatccgt caagcataac atctgctccc
      961 ggtatcccct ccgctgctcc ctgggcaaaa gccagaataa atgtggccgt catcggtccc
     1021 gaggccactc cccctgagtc aaatgcgatt cccacaaaaa gcttcggcac gatatatgtg
     1081 agtatgacgg ctataatgta tccgggcagc agatagtgcc acagatcaag atccggcaca
     1141 agtatcctta tcattgacag cgccaccgag gtgcccacgc caatggccag agcccccatt
     1201 acggtctttc tttttacata tccgctggta acgttttcta tctgatccgt cagcacataa
     1261 actgcaggtt ctgcaagtat tgtcaaaagc cccagaagaa aaccggtccc tatcagaaga
     1321 aaatcgtttt ccagagacgc tatctgaaag cccactatcg ttccgacttc catgaacccc
     1381 gcattgacac ctgtcaaaag cagaacaagt cccacgaaag aataaacgat accgagaaga
     1441 atatccttcg tctgttgttt gcctaattta aaagctctct tacgtagtat taggaaaaca
     1501 aggaggatcg ggagcaacgc aaaaaatact tcatatgcca taacaggcat tttatacata
     1561 aaaggatgga aaatacttcc gtcatccccg gctacaatgt ctacactgcc gcttatgtcc
     1621 tctgcaccta taatgaggct catgatcatg accgatatta ttgggcccgt cgaaactatg
     1681 gccacaagtc cgaaactgtc tttttctgat gcctgactgt cccttttgag agcagaaaca
     1741 ccaactgcca gagccagaat gaaggggact gtaagggctc ctgtggttgc tcccgatgca
     1801 tcaaaggaaa tagctataaa ttcaggtgaa gcaaatatgg ctatcgcaaa tattatcccg
     1861 taaagtatag tcagcatttt atggagtccg aagttatata ctattcgaag aagtcccgcc
     1921 gaaagcaaaa cagctatccc gaaagaaact ata
//
LOCUS       DAONHK010000174         1945 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_52316_length_1945_cov_5.204233, whole genome shotgun sequence.
ACCESSION   DAONHK010000174 DAONHK010000000
VERSION     DAONHK010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1945)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1945)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1945
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52316_length_1945_cov_5.204233"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1538
                     /gene="ftsH"
                     /locus_tag="PLM92_05700"
     CDS             <1..1538
                     /gene="ftsH"
                     /locus_tag="PLM92_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007749126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HPR24666.1"
                     /translation="YTQLGGKIPKGALLIGPPGTGKTLLAKAVAGEANVPFFSISGSD
                     FVEMFVGVGASRVRDLFKQAKEKAPCIVFIDEIDAVGRARGRNVNFGSNDERENTLNQ
                     LLTEMDGFGTNIGVIILAATNRADVLDRALLRAGRFDRQIHVELPDLTEREAIFKVHL
                     RPLKLEKNFDVSFLARQTPGFSGADIANVCNEAALIAARKNKKLVERQDFLDAVDRIV
                     GGLERKNKIISMDEKKTIAYHEAGHATVSWLLEHASPLVKVTIIPRGRALGAAWYLPE
                     ERQITTREQLLDEMAYSLGGRAAEELIFGKISTGALSDLEKITKQAYAMVSFFGMSEE
                     IGNISFYDSSGQSDYGFTKPYSEKTAELIDREVKMIIEESYERAKNVLRKNMKGLREL
                     AELLLEREVIFSEDLERIFGKRKADIKKEKEASEVEKAKNEKDLKEKEKEQTSPAISD
                     EKEIAVVKTDTENKAEEDIRKPEQEVKEEGEQKKEKNTNAKSGKKNKNTSDQEKLFPD
                     TTA"
     gene            1581..>1945
                     /locus_tag="PLM92_05705"
     CDS             1581..>1945
                     /locus_tag="PLM92_05705"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HPR24667.1"
                     /translation="MNNFFRRTLTGAWIVIFTLGGFWLHPLSFFLTGLIILTGSMLEY
                     YKIIQGSGAKPRIIPGIMSGMTSYIIAALAAAGYLPVRWLVLLIIPVPVIMITELFRK
                     ENKPFDSLAHTFFPFLYIVL"
BASE COUNT          657 a          351 c          459 g          478 t
ORIGIN      
        1 aatatacaca attaggagga aagataccta agggtgcctt gcttattggt cctcccggaa
       61 ccggcaagac tcttcttgcc aaagctgtag caggagaagc caatgtgccg tttttttcca
      121 tttcaggatc agactttgtg gaaatgtttg taggagtagg agcgtcaagg gtaagggatc
      181 ttttcaagca ggcaaaggaa aaagctccgt gtattgtttt tattgacgag atagatgctg
      241 ttgggagggc aagaggacga aatgtgaatt tcggatcgaa tgatgaacgg gagaatactc
      301 ttaaccagct tcttactgaa atggacggct ttggaaccaa cataggggtt atcatccttg
      361 cagctaccaa cagggcggat gtacttgaca gagctcttct cagagccgga agatttgaca
      421 ggcagataca tgttgaattg cccgacctta ccgaacggga agctattttt aaagtccatc
      481 tgcgtccgct taagctggag aaaaattttg atgtttcctt cttagccaga cagacaccgg
      541 gcttttcagg agccgacata gccaatgtgt gcaacgaggc ggcccttata gcagcccgga
      601 aaaataaaaa attggttgag aggcaggatt ttcttgatgc tgttgaccgc atagtaggtg
      661 gtcttgaaag aaagaacaag atcatctcta tggatgagaa gaaaacaatt gcctatcatg
      721 aagccggtca tgcaactgtc agctggctgc tggaacatgc cagtcctctg gtgaaagtga
      781 caattatacc gcgtggaaga gcactcgggg ctgcatggta tcttcctgaa gagagacaaa
      841 taactacaag agaacaactg cttgatgaaa tggcctactc tcttggtgga agggcagctg
      901 aagaactaat tttcggtaaa atatcaacgg gcgcactcag cgacctggag aaaataacca
      961 aacaggctta tgctatggtc tcattcttcg gaatgagcga agagatagga aatataagct
     1021 tctatgattc ttccggccag tcagattatg gatttaccaa accctatagt gagaagacag
     1081 ctgaactgat agacagggag gtgaaaatga ttatagaaga atcatacgaa agagctaaaa
     1141 atgtcctcag aaaaaatatg aaaggattga gggaactggc agaattgtta cttgaaagag
     1201 aggtgatttt cagcgaagat ctggaaagga tttttggtaa aagaaaagcc gatataaaaa
     1261 aagaaaaaga agcttctgaa gtagaaaagg ccaaaaacga aaaggatttg aaagaaaaag
     1321 aaaaggaaca aacttccccg gcaatcagtg atgaaaaaga aatagctgtt gttaagacag
     1381 atacagagaa taaggcagag gaagatataa gaaagccaga acaagaagta aaagaagaag
     1441 gcgaacaaaa gaaagaaaag aatacaaatg caaagtccgg aaagaaaaac aaaaatacat
     1501 ctgaccagga aaaattgttt cctgatacta ccgcatgata cagttcaata gaaaaatcca
     1561 ccaggtaaat ctcaactgac ttgaataatt ttttcagacg gacactaacc ggggcatgga
     1621 tagtaatatt tacccttgga ggattctggc ttcatccttt atcattcttt cttactggtt
     1681 tgataatact tacaggatca atgcttgagt attacaaaat aattcagggc tcaggggcaa
     1741 aacccaggat tatccccggt attatgtccg gcatgacatc ttatattata gccgctttag
     1801 cagctgcagg ttatctgcct gtacggtggc tggtcctgct gataatacct gtacctgtga
     1861 ttatgatcac tgaattattc agaaaagaga acaaaccatt cgattcactt gcacatacat
     1921 ttttcccgtt tttgtatata gtcct
//
LOCUS       DAONHK010000175         1886 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_54986_length_1886_cov_4.782086, whole genome shotgun sequence.
ACCESSION   DAONHK010000175 DAONHK010000000
VERSION     DAONHK010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1886
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54986_length_1886_cov_4.782086"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            262..1680
                     /locus_tag="PLM92_05710"
     CDS             262..1680
                     /locus_tag="PLM92_05710"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPR24668.1"
                     /translation="MKLSFKAKTGYAAAGIGDAASYTFIGTYLLFFLTTVVGMDPVMA
                     GTVSAIGAFWDTLWSPVMGFISDRSYTKWGRRIPFIFASAFPLAAANCFLFMTIDAGE
                     TFRFIYYSVMVIIFWTAFCAFFNPYLALGAEVTDDYHERTSLRGYTSIMNMVGMMVGM
                     VLPTVIVDALCNTGASLGCAWQTTGALIGFISLVSLMVTVFTMKTKDKDFKPTPRKKE
                     KRFLITGIKTMLREYKEIIMLKPLQKLVFVALVFLCANTIIASDRIYFLTYNLAFSPV
                     QITIVLSATMLMSMATTPAVIRLSRRSDKRSTFIGLMLFSSLCVFAAKFLGIDSMADM
                     LLLVFVFAWGNTVYWQLMPSMIYDVCEVDELYSGKRREGAISSLLSLSEAFSGAVSMQ
                     VMGFILGYVGFDGESATQVEPALSWIENLLTSVPGAIMAFSALLMLLYPVTKEKFDQV
                     MKALEKRRSGLKLDKSDYPIKK"
BASE COUNT          523 a          360 c          422 g          581 t
ORIGIN      
        1 gcacctgtgg ttctcgtttt ccattccttg aaatcattga atttccgcct ttcttcacac
       61 ttctattata ccatttcctg ggtttaaaga cttgggatcc aatgatgttt taaattttat
      121 gcaatagaaa aaagtccgtt gcctcgttgc cgaggtcgga cctttgtcta cagtctgaaa
      181 ccgccctgta aaagggcggt ttttttgata tgaatcgctt tctttgttga aacattaaaa
      241 gattactgct aaagtagaaa tatgaaacta tcttttaagg caaaaaccgg atacgctgca
      301 gcaggcatag gagatgcagc atcatataca ttcataggaa catatttgct gttttttctt
      361 actacggtgg tggggatgga cccggtaatg gccggtacgg tttccgccat cggagcattt
      421 tgggatacac tttggagccc ggttatgggc tttatttctg accgcagtta taccaagtgg
      481 ggcagaagga taccctttat attcgcctca gcttttcctc tggccgccgc caattgtttt
      541 ctttttatga caatagatgc cggtgaaacc ttcaggttca tttattattc cgtaatggta
      601 atcattttct ggacagcatt ctgcgctttt ttcaatcctt atttggctct gggagcggaa
      661 gttacggatg attatcacga gaggacgtct ctaaggggat acacaagcat catgaacatg
      721 gtcggaatga tggttggaat ggtgctgccc acggtcattg tggatgccct ttgtaatacg
      781 ggggcttctc ttggctgcgc atggcagaca acaggggcgc ttataggatt tatttctctc
      841 gtgtcactta tggtgacggt ttttacaatg aaaacaaaag acaaggactt taagccgaca
      901 ccgagaaaaa aagaaaaaag atttttgata accggaataa agacgatgct cagggaatat
      961 aaagaaataa taatgctcaa gcctttgcag aaactggtat tcgtagcact tgtctttctt
     1021 tgtgcaaata ccattattgc atctgacagg atatactttc ttacatataa tctggcattt
     1081 tctccggtgc agataaccat cgtgctttcg gcgaccatgc tgatgagcat ggccacaact
     1141 cccgccgtta taagactcag caggagatcc gataaaagaa gcacatttat cggtctgatg
     1201 cttttttcat ccctatgtgt ttttgcagcc aaattcctcg gaatagattc tatggctgac
     1261 atgctccttt tggtattcgt ttttgcatgg ggcaatacgg tttactggca gcttatgcct
     1321 tcaatgatat acgatgtatg tgaggtggat gaactgtatt ccggcaaaag aagagaagga
     1381 gccatatcct cacttctcag tctttcggaa gccttttcag gggctgtcag catgcaggtc
     1441 atgggattca tactgggata tgtgggattt gacggagaat ccgcaaccca ggtagaaccg
     1501 gctctttcat ggatagaaaa tcttcttaca tctgtaccgg gggcaattat ggccttttcg
     1561 gcacttctta tgctgcttta tccggtgaca aaagagaagt tcgaccaggt aatgaaagct
     1621 cttgaaaaaa gaaggtccgg gctcaagctg gataaatctg attatccaat aaaaaaatga
     1681 ggattttcta ttccgaatac tgttatatca ttacgaaatc cgcagatatt catatatcgt
     1741 ttgaatagaa atctaatatg aatagtaatt attgattata caaaaaaaag aagcggccat
     1801 ataatttaag tagacagtag tctgcttttt tattggtaat tatttgagaa atgagaggat
     1861 caatatgaga atagatgtta cagggg
//
LOCUS       DAONHK010000176         1869 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_55806_length_1869_cov_2.045755, whole genome shotgun sequence.
ACCESSION   DAONHK010000176 DAONHK010000000
VERSION     DAONHK010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1869
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55806_length_1869_cov_2.045755"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(10..1539)
                     /locus_tag="PLM92_05715"
     CDS             complement(10..1539)
                     /locus_tag="PLM92_05715"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HPR24669.1"
                     /translation="MLQIVTLITILLYIIMVVSIAIYCYRTTKTMDSFLLGSRKVGPW
                     VSAFSYGTSYFSAVIFIGYAGMFGWIIGAGSIMIGVGNAAFGSLVAWLLLAKPTRRMT
                     HRIKAKTMPEFFAKRYECRNLKIYAAIIIFLFLVPYAAGVYKGLGSLFSAVFPGVPEE
                     TIMLLVALLTATGLFLGGYKATSLIDFFQGIIMLTGVVAMIVVVINQPEVGGLFEGFQ
                     KLKSIDPDLISPFGGKKWFPLLINISLTSFAVWGLPQMIHKYYAIKDESSIKQGTVIA
                     TMFALVIGGGAYLVGTFGHLFVPALPDGTPDVTGSFDGVMPEILMSVFGSNIFLIILL
                     SIILLLLLSASMSTLEALVLSSSSAVAVDLLGTVRPGINEIKQMFIMRGFCVLFVALS
                     YIFATMNISFIVNLMSFSWGVVAGCFIGPFLWGLYWKGVTKMGAWAGALSGLVIVTGL
                     TLLNTFQEGFAAAKSMAPQFGVTAMIVSLVAVPLVSLFTKKFSSEHIDRCFKDGEKDN
                     G"
     gene            complement(1565..>1869)
                     /locus_tag="PLM92_05720"
     CDS             complement(1565..>1869)
                     /locus_tag="PLM92_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPR24670.1"
                     /translation="AFAYPYGDYTENCRNILQKAGFICAVTTKGGRVFPGNDSLLLPR
                     IRVVGGHDIYQFIGDISPGEPVMAGSWEAYYPDEINQNSVYDQDVGNDRDGGMSGQ"
BASE COUNT          569 a          467 c          337 g          496 t
ORIGIN      
        1 ttgcccatat taaccattgt ccttctcccc atctttgaaa catctgtcga tatgttcact
       61 gctgaatttt ttcgtaaaaa ggctcaccag cggaactgct accagtgaaa cgatcattgc
      121 tgtgactcca aactgcggag ccattgattt tgctgcagca aatccttcct ggaatgtgtt
      181 taacagcgtg agtccggtaa caataacaag accgcttaaa gcacctgccc atgcacccat
      241 ttttgtaaca cctttccagt ataggcccca caaaaaaggc cctataaaac atcctgccac
      301 aactccccat gaaaaagaca taagattcac tatgaagctt atattcattg ttgcaaaaat
      361 gtatgaaagt gccacgaaaa ggacacagaa ccctctcata ataaacatct gctttatctc
      421 atttatgccc ggtcttacgg tgcccagcag atccactgcc accgcagaac ttgacgaaag
      481 gaccaaagcc tcaagagtag acatggatgc cgacagcaac agaagcaata ttatactgag
      541 caatataata agaaaaatat tactgccgaa tacagacatg agtatttcag gcatcacccc
      601 atcaaaagaa ccggtaacat ccggtgtacc atcgggcaat gccggcacaa aaagatgccc
      661 gaaggtgccg accagataag cccctcctcc tatcacaagg gcaaacatgg tggcaataac
      721 tgtgccttgc tttatacttg attcgtcttt tattgcataa tatttatgta tcatctgggg
      781 caagccccat acagcaaaac tcgtcagaga aatatttata aggagaggga accatttttt
      841 tcctccaaag ggactgataa gatcggggtc aatgctcttc aacttctgaa aaccttcaaa
      901 caatccacca acttccggct gattaataac aactacgatc atggccacaa ctcccgtaag
      961 cattattatg ccctggaaaa aatctataag agaagtagct ttgtatcctc cgagaaaaag
     1021 ccccgttgcc gtaagaagag ccaccagcaa cataatcgtc tcttcgggca ctcccgggaa
     1081 taccgcggaa aaaagagacc caagtccctt gtaaactccc gctgcatagg gtaccagaaa
     1141 cagaaaaatt attatggctg catagatttt aagattgcgg cattcatatc tttttgcaaa
     1201 gaattccggc atcgtttttg cctttatcct gtgagtcatc cttcgggtgg gctttgcaag
     1261 aagaagccat gctaccagtg aaccgaaagc tgcgtttccc acacctatca tgatactgcc
     1321 tgcaccgatt atccagccga acatcccggc ataacctata aatataactg ctgaaaaata
     1381 tgatgtccca tatgaaaagg ccgataccca ggggccgact ttcctgcttc ccaaaagaaa
     1441 agaatccatt gtctttgtag ttctatagca atatatggca atggatacga ccataattat
     1501 atataacaat atcgttatca atgttacgat ctgaagcatt ttttcttttt cctttcactt
     1561 tttgttactg tccgctcatg ccgccatccc tgtcattgcc gacatcctga tcataaaccg
     1621 aattttgatt tatttcatcg ggataatagg cttcccagga acctgccatt acaggttctc
     1681 ccggcgaaat atcccctatg aactgatata tatcatgccc tcctactact cttattctgg
     1741 gcaaaagaag agaatcattc ccgggaaaaa ctcttccgcc tttagtagtg acagcacata
     1801 taaaccctgc tttttgtaat atgttcctgc agttttctgt ataatctccg tagggatatg
     1861 caaaggctt
//
LOCUS       DAONHK010000177         1828 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_57736_length_1828_cov_3.075578, whole genome shotgun sequence.
ACCESSION   DAONHK010000177 DAONHK010000000
VERSION     DAONHK010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57736_length_1828_cov_3.075578"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1072)
                     /locus_tag="PLM92_05725"
     CDS             complement(<1..1072)
                     /locus_tag="PLM92_05725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPR24671.1"
                     /translation="MRKSFIFIIAVVVTAATLFFTFNFSGCTASLDEASASQLKNDVY
                     DIYEELKPIYLDSKDIYEVRDHMYDWGKKQGFSVSKLAGGNLVITKHPSRNKKDSPET
                     VFQCTMSQDNYKEHCQTAAIALAAMKNASIHGQVSLILTADIEGKHTGAKMLSTSYLN
                     TDYFMNLEYSPETAVYTGSAATAEYRMSCPLTKSYAAADKAYEIKITGLSCVDSSDRS
                     KKHPNPIVTIADLLTNCKSGGLIVEMAEFNGGTSACKFPSSATAVIVVDQSSEAKFLK
                     KLEKCQDDFTDDYYNDQPNMDFEFKEVKVPSKVISEKYTSNILSLMYTTVNGTYSTTE
                     EDGEGDPTALANKGKISTKDDFE"
BASE COUNT          522 a          352 c          411 g          543 t
ORIGIN      
        1 gctcaaaatc atcctttgtg gagatcttgc ccttattggc aagagcagta ggatcgcctt
       61 ctccgtcttc ttcggtggtg ctgtatgtac cgttgactgt ggtatacata aggctcagga
      121 tattggaagt gtatttttcg gatatcacct ttgatggaac ttttacttct ttgaattcaa
      181 aatccatatt gggctggtcg ttataatagt catcggtaaa atcatcctga catttttcaa
      241 gtttctttaa aaatttcgct tcgctgctct gatcgaccac gatgacagca gtagcggagg
      301 aagggaattt gcaggcggat gtgcctccgt tgaattcggc catttctacg ataagtcctc
      361 cggatttgca gttggtaaga aggtcggcga tagttacgat ggggttggga tgttttttgc
      421 tgcggtccga agaatccaca catgaaagcc cggtgatttt tatctcataa gctttgtcag
      481 cagcggcata tgattttgtc aaggggcagg acattctgta ttccgccgtg gccgcgcttc
      541 ccgtgtaaac cgccgtttcg gggctgtatt ccagattcat gaaataatcg gtgttaagat
      601 aacttgttga aagcattttg gcaccggtat gtttcccttc tatatctgca gtaaggatca
      661 gggagacctg accgtggata gaagcatttt tcatggcagc aagggcgatg gccgccgttt
      721 gacagtgctc cttatagttg tcctgtgaca ttgtgcactg gaaaacagtt tcgggagagt
      781 cttttttgtt tctggaagga tgtttggtta ttacaagatt gcctcctgcc agttttgata
      841 cagaaaaacc ctgcttcttc ccccagtcgt acatatgatc acggacctca tatatatctt
      901 ttgaatcaag atatatgggt tttaattctt catatatatc gtaaacatca tttttcagct
      961 ggcttgcaga cgcctcatca agagaagcag tacatcctga aaaattaaat gtaaaaaata
     1021 aagttgcagc tgtcacaaca acagctatta tgaaaataaa actttttctc atgcatcatt
     1081 cctccaaagc accatacaat attaccatat acaaaaaaga ttttgtatag atttcatgaa
     1141 attttccaca cggggacagt ttttggatcg ggaataaaag gttctttgcc agagatcacg
     1201 ggcatgaata tggtttcata agagcattat agagcatcat aagggtttca taggtgttgc
     1261 ataagggttt cataggtgtt gtattaggct ttgagagggc aaatcgtatg aaatcattgg
     1321 ctttttcgtt ttttttctgt ttcctgattg acatcccaac tacaaagggt ataatttatt
     1381 tataagcgac ataatacaca caacatattg tattttggct aaaaaaagcg aattatagcg
     1441 agtaaaagct aacaacagcc agcaacagcg aacaacagtg aacaacagcg aatgattttc
     1501 aagcatgatt tcctgtctta cgatctgtaa gttgccgaag cccgggaact ctttctgtca
     1561 ggcggaagat atcccggcaa tttcggaaaa gccagcaggc aacctgaaac ctgcaacagt
     1621 caccagacat tggacctgca gcagtaagga tgcatggcag tactgagaaa tattccttga
     1681 cggaaaatat gcgcgcatgg tataatgcca cttgttctga caaaacaagt tctggcaaaa
     1741 taagcataga actttcagat atgagcttat atgaaaagga ggtgcggcag aatgtcaaga
     1801 aaatgtgaag tttgcggcaa aggacagg
//
LOCUS       DAONHK010000178         1814 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_58427_length_1814_cov_3.723707, whole genome shotgun sequence.
ACCESSION   DAONHK010000178 DAONHK010000000
VERSION     DAONHK010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1814
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58427_length_1814_cov_3.723707"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..675
                     /locus_tag="PLM92_05730"
                     /pseudo
     CDS             1..675
                     /locus_tag="PLM92_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012103736.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid permease"
     gene            781..1368
                     /locus_tag="PLM92_05735"
     CDS             781..1368
                     /locus_tag="PLM92_05735"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HPR24672.1"
                     /translation="MKERKLYLIRHGITEGNINKYYYGRTDVSLAPEGITELKEFSEK
                     GIYPMPAPDFVFTSGMQRTEESLSVIYGDTRHHILEELKELDFGDYEMRSHGELMETA
                     EYSDWFASGSERTGFPGGEDLIAFERRVMKGYEKMLSAFDEEGMAVCHGGVISVIMMN
                     CFPDPQMNLFSWIPEPGRGFVIKLKEEEAVNWERI"
BASE COUNT          550 a          357 c          453 g          454 t
ORIGIN      
        1 aaaaaagaat ctgccaaggg ctttgctggg aggcaccata cttatcgtta ttatatatat
       61 cgtttattat ataggccttg caggggcagt ttccaatgaa accatgatgg ccggaggcga
      121 agccgcagca aagatggcct ttgagaccat attcagcagt gttgcgggga ctttgatatt
      181 cgtacttata gttgtatcct gcctgggcac attgaacggt ctgatgctgg gatgtaccag
      241 agggctttat tccatagcag taagaaaaag aggacccaga ccggagatat tcaaccaggt
      301 ggacaaaagt actaacatgc ccaccaatgc ttctatagca ggagttatgc tttgcggttt
      361 ctggctgctt tacttctacg gagcaaacct tacggcacca tggttcggac ctttctgttt
      421 cgacacatcg gaactgccga tagttaccat atacgctctt tatataccca tattcatcat
      481 gatgatgaaa aaagaaaaga ttttcccaca ttcaagagat tcgttgcgcc ggctctggca
      541 ttatgcggaa gccttttcat gatagtagca gcatgttttt cccacaggat ggctgttgtg
      601 tattatctga tagtgtttgc cgtggtaatg atatggggcg ctgcattaca gaagaaaaag
      661 gaaatatccg actgagctga tgccggtaac aggccggcag aatgacagta tcgacaaatt
      721 caagggtgcc gtttcggttt gttgcggcac ccgttgtatt ttttataaaa ggatccggca
      781 atgaaagaac gaaaactgta tctgataaga cacggtatca cagaaggcaa cataaacaaa
      841 tattattacg gcaggaccga tgtttccctg gcccctgagg ggataacaga actcaaagag
      901 ttttccgaaa aaggtatata tcccatgcct gctccggact ttgttttcac gtcggggatg
      961 caaagaaccg aagagtccct ttctgttata tacggcgata ccagacatca tatactggag
     1021 gaacttaagg aactggattt cggggattat gaaatgagat cccacggaga actcatggag
     1081 accgccgagt attccgactg gttcgcatcc ggcagtgaaa ggacggggtt ccccggcggg
     1141 gaagatctga tcgctttcga gagaagggtc atgaaaggat atgaaaaaat gctgagtgcc
     1201 ttcgatgaag aaggtatggc ggtatgccac ggcggcgtta tatctgtaat aatgatgaac
     1261 tgtttcccgg atccccaaat gaacctgttt tcatggatcc cggagcctgg caggggtttt
     1321 gttataaaac tcaaagagga agaagcggtg aactgggaaa ggatatgatc ttgatatctc
     1381 cctgatcttg aatgtttttg cggcagtgga acaggaaccg aaaaaatctg aaaaagtccg
     1441 aaagtttgaa gaagacgtaa ataagaaaga gaaaagacca aaataaattt ccgtacacaa
     1501 aaaccggcca ataaattttc cgttttatat attcggtttt tcatatcatg tgttataata
     1561 ctgtatacaa agtcatttcc ggagggcgaa aaagtgctat gtccgctgct ttgaagcagc
     1621 tatgaacata gcttcaaaac gataaagccg ctcaaatccg ggaaaaacaa aactgaaatc
     1681 acaggaggca gcataaatga tagacagaag tatgataaaa aaagaagact ggctgggaat
     1741 gggcgccgaa tctcccgggc aggcaaaagt atgcatgatg ggcataccct atgatggagg
     1801 cagtctgctg ggca
//
LOCUS       DAONHK010000179         1798 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_59320_length_1798_cov_2.748135, whole genome shotgun sequence.
ACCESSION   DAONHK010000179 DAONHK010000000
VERSION     DAONHK010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1798
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59320_length_1798_cov_2.748135"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="PLM92_05740"
     CDS             complement(<1..369)
                     /locus_tag="PLM92_05740"
                     /inference="COORDINATES: protein motif:HMM:NF017911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPR24673.1"
                     /translation="MTARALSGEHAYVTIEKDIKEERIPGILILCGREQYLVKWAIDL
                     LIKKYVEPASKEMDLFISEDTEITIDEIKSVCETLPILSPKKLVVLKDYAPVENERVI
                     EYLEDLPESTILVLTYEEGAG"
     gene            complement(380..1318)
                     /gene="trxB"
                     /locus_tag="PLM92_05745"
     CDS             complement(380..1318)
                     /gene="trxB"
                     /locus_tag="PLM92_05745"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HPR24674.1"
                     /translation="MDKIYDVIIIGAGPAGLSAGLYAGRAGLETLIIEKEEEGGQISQ
                     TAEIENYPGSMEEESGYSLTERMSKQACDFGAVIVKDTVKEISPEWEIKTLMGEKKDY
                     KTKTVIIAAGTSPLRIGCPGEGELIGKGISYCATCDGAFFQDMDVYVVGGGNSAVEEA
                     IYLTRYAKKVTVIHRREKLRAEKAIQEKAFANEKIEFIWNSVVKELKGEGFLETIITE
                     NIISREKTEIKAEGQDSSIGLFVFIGSDPKTEIFQKHMKTEKGYIITNDDMETEIPGV
                     YAAGDVRVKNLRQVITAAADGATAAFNAAKYIEREI"
     gene            complement(1355..>1798)
                     /locus_tag="PLM92_05750"
     CDS             complement(1355..>1798)
                     /locus_tag="PLM92_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPR24675.1"
                     /translation="KQQSIYREELSEDEENKSSLVIKITVEGYDILMTGDIDGETEEK
                     IVAEYKNSQKLRADILKVSHHGSRFSSSDNFLDAVAPKMAVFQVGKNNYGHPSSDVIE
                     KCRKKGIMIFRNDKSGAIGFIWSDGREELHIKKVIKDQQDRDRIY"
BASE COUNT          398 a          452 c          284 g          664 t
ORIGIN      
        1 tcctgccccc tcttcatatg tgagtaccag tatcgtgctt tccggcagat cctccagata
       61 ttctataact ctttcattct caacgggtgc atagtctttt aataccacca gtttttttgg
      121 cgaaaggatg ggcagagtct cacaaacact ttttatttca tcaatggtta tttccgtatc
      181 ttctgatatg aaaaggtcca tttcctttga tgcgggttcc acatattttt tgataagaag
      241 gtctatggcc catttgacaa gatactgttc ccgaccgcaa agaataagaa tacccggtat
      301 cctttcttct tttatgtctt tttcaattgt tacataagca tgttccccgc ttaaggctct
      361 ggctgtcata ctcttttttc tatatctctc tttctatgta ttttgcggca ttgaaagcag
      421 ctgtcgcccc gtccgcagca gccgtgataa cctgcctcag gtttttgacc cttacatctc
      481 ccgccgcata tactccgggg atctctgtct ccatatcatc gtttgtaatg atatagccct
      541 tttctgtctt catatgtttt tgaaatattt ccgtctttgg gtccgaccca atgaatacaa
      601 acaatcctat tgagctgtcc tgcccctcag cttttatttc tgttttttct ctgctgataa
      661 tgttttctgt tattattgtt tccagaaaac cttctccttt cagttctttt acaacactgt
      721 tccaaatgaa ttcgattttt tcattggcaa aagccttttc ctgtatggct ttttctgcac
      781 ggagtttttc ccttctgtgt attaccgtca ctttttttgc atatcgggta agatatatcg
      841 cctcttcaac tgccgaattt cctcctccca caacatatac atccatatcc tgaaaaaaag
      901 ctccgtcaca tgtggcacaa taggagatgc ctttccctat aagctctcct tcgcccggac
      961 atcctatcct taacggactt gttcctgctg cgatgataac ggtcttagtt ttataatcct
     1021 tcttttctcc cattaaagtt tttatttccc attcaggtga aatttcttta accgtatctt
     1081 tgactattac cgcaccgaaa tcgcatgcct gctttgacat tctctcggta agggaataac
     1141 cggattcctc ttccatgctc ccgggataat tttctatttc tgcggtctgt gatatctgtc
     1201 cgccttcttc ttctttttca attatcagtg tttcaaggcc ggcccggccg gcataaagac
     1261 cggcagaaag ccccgcaggt ccggctccga ttataatgac atcatatatt ttgtccataa
     1321 tatccttctg ttccttccgc ttttatttct ataatcaata tattctatcc ctgtcctgct
     1381 gatctttgat gacctttttt atatgaagct cctctcttcc atccgaccaa ataaaaccta
     1441 tggctccgga cttgtcattt ctaaatatca ttataccttt tttacgacac ttttcaatga
     1501 catcactgct gggatggccg taattatttt tgccaacctg aaacacagcc attttggggg
     1561 ctactgcatc cagaaaatta tccgaactgc tgaaacggct cccgtgatgg gatactttaa
     1621 gtatatctgc tctaagtttt tggctgtttt tatattctgc tacaattttt tcttccgttt
     1681 caccgtctat atcccctgtc ataagaatat cataaccttc gacagtgatc tttattacaa
     1741 gactgctttt gttctcctca tcttcggaaa gctcttctct atatatgctt tgctgttt
//
LOCUS       DAONHK010000180         1754 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_61760_length_1754_cov_2.273102, whole genome shotgun sequence.
ACCESSION   DAONHK010000180 DAONHK010000000
VERSION     DAONHK010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1754)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1754)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1754
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61760_length_1754_cov_2.273102"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1097)
                     /locus_tag="PLM92_05755"
     CDS             complement(<1..1097)
                     /locus_tag="PLM92_05755"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPR24676.1"
                     /translation="MFGFIVSIIGSLFLMFIAIPYPSYPTVLSALTLTGLGVGFTMGA
                     PLNYMMLANTRKEESATALATLSLVRSIGTTIAPAIMVAFLAHAGSTLQSDVMDILPK
                     EVSVPKLPYAKQLDDKLSEFKSDPSYSEEFADMDIPDLGDMTKVKIKAGKSDAKIPED
                     VLDLLKTSDVTNITKRTERFASEMFDIETPSVIKEINSGMQSGIDAIKDSENELTDEV
                     SSMEKAVSGIDKGIMGMKKAVSGIREGTEGIERAIVGQQKMLAVLKQLYGQISSAPVP
                     AGGAGSFADITEMIPAYILDRLPENVINELRSIKTPEELKKKIDSTEKTIDILSAKKV
                     QMDAKVYSLKADIRQTEAKKAGLLKAIDGMK"
     gene            complement(1175..>1754)
                     /locus_tag="PLM92_05760"
     CDS             complement(1175..>1754)
                     /locus_tag="PLM92_05760"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPR24677.1"
                     /translation="ATAEFGTIFPKEKQGMALGLVGGVYGIANIFGASAGSAVLDIFG
                     RNSWQLIFYINVPISIFIIIAGILFLPNTRVESVKKIDFSGILILVLIIISLLYGLKN
                     IDFFDLSNTMRQTDVFPFLLAVVILLPIFIIVEKRAEDPAMNLQYFRTLPIVITMVVA
                     MISGMLLMGMVFVPQFSENAMKIPSGEGDISL"
BASE COUNT          443 a          414 c          379 g          518 t
ORIGIN      
        1 tttttcatgc cgtctatagc cttaagaagt cccgcttttt tggcctctgt ctgcctgata
       61 tcagctttta gggagtagac ctttgcatcc atctgtactt ttttggccga caggatatct
      121 atggttttct ctgtggaatc tatctttttc tttagttctt caggtgtttt tatgctcctg
      181 agttcattga tcacattttc cgggagccgg tcaaggatat atgcaggtat catttccgtt
      241 atatcagcaa atgatcctgc tcctcctgcc ggaacaggtg cagaggatat ctgtccgtaa
      301 agctgcttga gcaccgccag cattttctgc tggcccacga tggctctttc gatgccttct
      361 gttccttccc ttatgccgga tacagctttt ttcataccca tgataccctt atcaataccg
      421 gagaccgctt tttccatgct gctgacttca tctgtcagtt cattttcgga gtctttgatg
      481 gcatcgatac cgctctgcat tccggaattt atttctttta ttacagaagg cgtttcaata
      541 tcaaacatct cggatgcaaa tctttcggtc cttttcgtta tatttgtgac atctgaagtt
      601 ttcaaaagat caaggacgtc ttcaggtatc tttgcatcac ttttgcctgc tttgatctta
      661 actttagtca tgtcgccaag atcgggaata tccatatctg caaattcttc actgtatgac
      721 ggatcacttt tgaattccga aagtttatcg tccagctgtt ttgcataggg aagttttggt
      781 acggacacct cttttggaag gatatccatc acatcgctct gcagagtgct gccggcatgg
      841 gcaagaaatg ccaccatgat ggcaggagca atggttgtgc caatggatct taccagagac
      901 aatgtggcca gtgccgttgc cgactcttcc ttacgggtat ttgcgagcat catataatta
      961 agcggcgctc ccatggtgaa gcccaccccg agacctgtaa gggtaagggc gctcagaacc
     1021 gtgggataag acggataagg gatcgctatg aacatcagga aaagggagcc tattatggat
     1081 actataaaac cgaacatcag tacaagcttg gggcctatgc ggtctgtaag ttttcccgat
     1141 aaaggagcac ctactccagc aaacagtccc agagctataa cgaaatatcc ccttctcctg
     1201 agggtatctt catggcattc tcggaaaact gaggaacaaa taccattccc atcaaaagca
     1261 tgccggagat catggccacc accatggtta ttactatggg cagggtgcga aaatactgaa
     1321 ggttcatggc ggggtcttcc gctctttttt ccactattat gaagatcggc agcagtatca
     1381 ctaccgccag aaggaaagga aatacatccg tttgacgcat ggtgtttgac aaatcgaaaa
     1441 aatctatgtt cttaagcccg taaagcaatg aaatgattat caaaacaagg atgagtatcc
     1501 ccgaaaaatc tatcttcttc acgctttcta cccttgtatt gggcagaaaa agtatgcctg
     1561 cgattattat gaatatactg atcggcacat tgatataaaa tataagctgc cagctgtttc
     1621 ttccgaagat atcaaggaca gcgctgcctg ccgaagctcc gaagatgttt gcaatgccgt
     1681 aaacaccgcc caccaggcca agagccatgc cctgtttttc ctttggaaaa atagtgccga
     1741 attccgctgt ggcc
//
LOCUS       DAONHK010000181         1682 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_65987_length_1682_cov_3.407498, whole genome shotgun sequence.
ACCESSION   DAONHK010000181 DAONHK010000000
VERSION     DAONHK010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65987_length_1682_cov_3.407498"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            17..>1682
                     /locus_tag="PLM92_05765"
     CDS             17..>1682
                     /locus_tag="PLM92_05765"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HPR24678.1"
                     /translation="MLLKAGDKIPADMRLIDVSRFEVDESSLTGESVAVSKSTKEVEQ
                     GTVLGERSSMAYAGTTVRTGSAKGIVTATAADTEVGKINTMLSETKATTTPLTRKINK
                     LGKNLSFIILAFSVLLMLYAVFVNDASLPNTIIAVIGLAVAAIPEGLPAVITITLAIG
                     VQRMARRNSIVRRLPSVETLGSVTVICSDKTGTLTKNEMTATNIYTAKGEYAVSGPGY
                     APEGEITKEGKKADIKSDGLLFKLIEISDLCNDAEIVYENNTWIPHGAPTEAALKALA
                     QKTGYTKEGYVKEDEIPFDSEYKYHATLYKRSGKKLILANGAPERLIAICGLQETDKG
                     RENVNRVYWEETIEMAAARGQRLIGCAYAEVPDEKSNISHEDMNGEMVFAGIVGIIDP
                     PRPEAIESIRICKNAGIRVKMITGDHVLTASEIGTQMGLTDRKKVVSGSELEEMDDDQ
                     IREAVSECDIFARTSPEHKLRLVKALQEQDEIVAMTGDGVNDAPALKKADIGVAMGIK
                     GTEVTKDSAEMVLADDNFSSIVSAVEEGRTIYDNIRKALLFILPTNG"
BASE COUNT          560 a          300 c          438 g          384 t
ORIGIN      
        1 ggtgcccgga gatttggtgt tgttaaaggc gggagacaaa atacccgcag acatgcgtct
       61 tattgatgtg agccgcttcg aggtggacga atcttctctt acgggagaat ctgtggctgt
      121 ttccaaatcc acaaaagaag ttgaacaggg aacggttctt ggggaaagat cctccatggc
      181 atatgcggga acgaccgtaa ggaccggaag tgcaaagggt atagtaacag ctacagcagc
      241 agacactgaa gtaggtaaaa taaacactat gctttcggaa acaaaagcta caacaactcc
      301 actgacaaga aaaatcaata agttggggaa aaatttatcc tttataatac ttgcgttttc
      361 ggttttattg atgttatatg ctgtttttgt aaacgatgca tcacttccga atacaattat
      421 cgctgtcata ggacttgccg tggctgccat acctgaaggt ttgcctgcgg taattacaat
      481 aacgctggct attggagttc agcgaatggc aagaaggaat tcaatcgtaa gacggcttcc
      541 ttcagtagaa actctgggtt ctgtcacagt tatatgttcc gataaaacag gaactcttac
      601 aaaaaatgaa atgacagcaa caaatatcta tacggcgaaa ggggagtacg ccgtatcagg
      661 accgggatac gctcctgaag gagaaataac aaaagagggg aaaaaggctg acataaagag
      721 tgatggtttg ttattcaaat taatagaaat atcggatctt tgcaacgatg ctgaaatagt
      781 atatgaaaat aatacatgga taccacatgg agcacctact gaagcggccc tgaaggcact
      841 tgcacaaaaa accggttata caaaagaagg atatgtcaaa gaagatgaga taccctttga
      901 ttccgaatat aaataccatg cgactttata taaaagaagc ggcaaaaaac ttattctggc
      961 aaatggggct cccgagcggc ttatagccat atgcggtctt caggaaaccg ataaaggcag
     1021 ggaaaatgtg aacagggtat actgggaaga gacaatagag atggctgctg ccaggggaca
     1081 gagacttatc ggttgcgcat atgcggaagt gccggatgaa aaatcaaata taagccatga
     1141 agacatgaac ggggaaatgg tgtttgcagg catagttggg ataatagatc ctccaaggcc
     1201 cgaggcgata gaatccataa gaatatgcaa aaatgcgggg atacgtgtaa agatgatcac
     1261 gggggatcat gttcttaccg ccagtgaaat agggacacag atgggtctta ccgacagaaa
     1321 gaaagtggta agtggatcag agcttgaaga aatggatgac gatcagatac gtgaagcggt
     1381 cagtgaatgc gatatatttg cgaggaccag ccctgaacac aaactaagac tggtaaaggc
     1441 ccttcaggag caggatgaga tcgttgcaat gacaggagac ggtgttaatg atgcacctgc
     1501 actgaaaaaa gcggatatcg gtgttgccat gggcataaag ggcacggagg tcaccaagga
     1561 ttccgctgaa atggtgctgg cagatgataa tttttcatca atagtttcgg ctgtggagga
     1621 agggaggacc atatatgaca atataagaaa ggctctttta ttcatactgc ccacaaatgg
     1681 tg
//
LOCUS       DAONHK010000182         1678 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_66297_length_1678_cov_2.105360, whole genome shotgun sequence.
ACCESSION   DAONHK010000182 DAONHK010000000
VERSION     DAONHK010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66297_length_1678_cov_2.105360"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            57..611
                     /locus_tag="PLM92_05770"
     CDS             57..611
                     /locus_tag="PLM92_05770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPR24679.1"
                     /translation="MKIFVISDTHGRIKKAVEVYKKLTDVDLMVHLGDYKRDALDLSS
                     KLGIDTVFVEGNMDGSTTRKSSSKIIDTEYGRILLTHGHLDNVKNGFENITARCISEG
                     CKAVVFGHTHRPLYEEKNGIYLLNPGSISVPRDGSSGSYAIIHTSADEFRASVVYCDN
                     KNSPPGSKQRGGYLKRLLNYSDRF"
     gene            complement(673..1125)
                     /locus_tag="PLM92_05775"
     CDS             complement(673..1125)
                     /locus_tag="PLM92_05775"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HPR24680.1"
                     /translation="MEGQKKMEAYMEKTMKENNVVQKDILNGMMRSENFSCSYEEKTV
                     TLDFPMQPWQANRVGFMHGGAICTAFDITMAALARFYARENFAPTVSIDLKYIRPVKM
                     GDTVLVTAKALSSGKRITQIACEAKSKNTGKTVATGASVYLNIDTTKE"
     gene            complement(1129..1551)
                     /locus_tag="PLM92_05780"
     CDS             complement(1129..1551)
                     /locus_tag="PLM92_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013781777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase"
                     /protein_id="HPR24681.1"
                     /translation="MFIKQVSVFLENETGRLSDYTKILKDNNIDMKAVCIADTVDFGI
                     LRCIVDDPDKAVNVLNENGFTASITTVIAVSFENVPGALHSVLEKLSDEGIGVDYIYS
                     MIRSETGKATLIFKVGDPEKAGKVLKKNDISLLCMKDI"
BASE COUNT          455 a          387 c          327 g          509 t
ORIGIN      
        1 agctcagtga acttgaaggt atttccataa tagacgagat ataaggacga aaaaatatga
       61 aaatcttcgt tataagcgat acccatggcc gtatcaaaaa agccgttgaa gtatataaaa
      121 agctcacaga tgtggatctg atggtacatc tcggggacta taaaagagat gcccttgatc
      181 tttcatccaa actgggcatt gatactgttt ttgttgaagg aaatatggac ggttcaacga
      241 ccaggaaaag ttcctcaaaa atcatagata cggaatacgg caggatactg ttgacccacg
      301 gccatctgga taatgtaaaa aacggatttg aaaatattac tgcccgctgc atttccgaag
      361 gatgcaaagc tgtggtcttc ggccatactc accgtccttt atatgaagaa aagaacggca
      421 tatatctttt gaacccggga agcatttcgg tgccccggga cggcagctca ggctcctacg
      481 ctattatcca tacttcagcc gatgagttca gggcatctgt tgtgtactgc gataataaaa
      541 acagcccccc cggcagcaaa caaagagggg gctatctaaa acgtctttta aattacagcg
      601 accgtttctg acaaacatgg ccggtttgat aaaagcatac cgcataagta acagcaactg
      661 ttcgcttttg aatcattcct ttgtggtatc gatattaagg tatacggagg ctccggtagc
      721 aactgtctta ccggtgtttt tactttttgc ctcacacgct atctgtgtta ttcttttgcc
      781 tgaagaaaga gcctttgcag tcaccaaaac cgtatcaccc attttgaccg gtcttatgta
      841 tttaagatca atgctcactg taggtgcaaa attctcccgg gcataaaatc ttgccagggc
      901 cgccattgtt atatcaaagg ccgtgcatat ggctcctcca tgcatgaagc ccactctgtt
      961 tgcctgccaa ggctgcatgg ggaagtccag cgttacggtt ttttcttcat aagagcaact
     1021 gaaattctcc gaacgcatca taccgttaag gatatctttt tgtactacat tgttttcctt
     1081 catggttttt tccatgtagg cttccatttt cttctgacct tccattttct aaatatcctt
     1141 catacacaaa agtgagatgt cgtttttctt aagaacctta cctgcttttt ccgggtctcc
     1201 aactttgaat atcagagtgg ctttgccggt ctcggatctt atcattgaat agatataatc
     1261 tacacctata ccttcatcgg acagcttttc aagtacagag tgcagggctc ccggcacatt
     1321 ttcaaaactt acggcaatga ccgtggttat actggcagtg aagccatttt cattcagtac
     1381 attgaccgcc ttgtccgggt cgtctactat acatctcaaa ataccgaagt ctactgtgtc
     1441 tgctatacat accgctttca tgtcgatatt gttgtccttc agtattttgg tatagtcgga
     1501 cagtcttccc gtttcattct ccagaaaaac cgaaacttgt ttaataaaca tattctcctc
     1561 ctctgctaaa ttatactctt tcagaaagat ttcttgtatc ttcaactctg actgctttgc
     1621 cttcgctcct tggcagagtt ccttcattca ggaaccttaa tgtacaaccg ataccaag
//
LOCUS       DAONHK010000183         1667 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_66961_length_1667_cov_2.887097, whole genome shotgun sequence.
ACCESSION   DAONHK010000183 DAONHK010000000
VERSION     DAONHK010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66961_length_1667_cov_2.887097"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="PLM92_05785"
     CDS             complement(<1..223)
                     /locus_tag="PLM92_05785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPR24682.1"
                     /translation="MRKKLRLAIVFLLIVAVSAGCGATGPGSAVMQKNNTKVRAEVDE
                     KSAAAVFDLAKENPEKGAEALNDIEDKATY"
     gene            complement(253..>1667)
                     /locus_tag="PLM92_05790"
     CDS             complement(253..>1667)
                     /locus_tag="PLM92_05790"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HPR24683.1"
                     /translation="SVIYVSSKGTIKAKKKGTATITATAKDGSKKKDSVKVTVGKKVK
                     KVSWKNASSKKTIYKGKSFNFNSKCTPSSASNRSVKYETSSKSIATVTSNGIVKGKKN
                     GTVTIKATTTDGTKKTVSCKVTVKTRVTSVKLSSGNLYDLANNDGIDAAGKTKFSYTV
                     YPSSASNKSVKWTSSNTAVALVDCNGYVTAQGPGSAKITVKAKDGSGKYSSKTVTVHN
                     IDKNSSAVFVAHRGYRGLAPENSIPAFREAYEKGFGAVETDVWENKDGSIVLFHDEYL
                     GRMCSAPSKRVKDLTNDQIEEYPLTAGVNIENYENLIIPGFQEFLENIGTKPAVIEIK
                     DTNISDGNIDEIIDEVSGRPGSMVISFYRNVLETVKKRAQLKNVEVTTMLLMSDSERT
                     TEQNIYSVKDGVNVCKDDEIDIDIISARYDMIGAEDVIYAHENDVKVGAWTVNDAGIA
                     LKIMGWGVDYITSDCKLFYN"
BASE COUNT          370 a          395 c          300 g          602 t
ORIGIN      
        1 cgtaggttgc tttatcttct atatcattaa gggcctctgc gcctttttcc ggattttctt
       61 ttgctaaatc aaatacggcc gcggcacttt tttcatccac ctcggctctt acttttgtat
      121 tattcttttg catgaccgcc gaacccggtc ctgttgctcc gcagcctgct gaaacagcta
      181 ctatgagcag gaacacaatg gccagcctaa gtttttttct cattttccct taccctttta
      241 tgccacaaac ggtcagttat aaaacaattt acagtctgat gttatataat caacgcccca
      301 tcccataatt tttaaagcga tccctgcgtc attcactgtc caggccccaa ccttcacatc
      361 attctcatgg gcatatataa catcttccgc tcctatcata tcatacctgg ctgatataat
      421 atcaatatca atttcgtcat ctttgcaaac gtttaccccg tcttttactg aatatatatt
      481 ttgctctgtg gtcctttctg aatcggacat gagaagcatc gttgtaactt cgacattttt
      541 taactgggct ctcttcttta ctgtttccag tacatttctg taaaaactga tcaccatgga
      601 gcctggtctt ccggaaactt catctattat ttcatcaata tttccatcgg atatgttcgt
      661 atcttttatt tcgatcaccg ccggttttgt tcctatattt tcaaggaatt cttgaaaacc
      721 cggtataatc agattttcat aattttctat attgacccct gccgtaaggg ggtattcttc
      781 tatttggtca ttcgtaagat cctttactct ttttgaaggt gcagagcaca tccttcccag
      841 atattcatca tgaaaaagaa ctatgctccc gtctttgttt tcccatacat cggtctcaac
      901 agctccaaat cccttctcat acgcctcccg gaaggcggga atgctgtttt ccggagcaag
      961 ccctctgtat cccctgtgtg ccacaaaaac agcgctacta tttttgtcga tgttatgtac
     1021 agtaacagtc ttcgatgaat acttgccgga tccatctttt gcttttacgg ttatcttggc
     1081 actgccgggg ccttgcgctg tcacatagcc attgcagtcc accaatgcca cagcagtgtt
     1141 tgatgaagtc catttgactg atttgttgct ggcggatgac ggataaacag tatatgaaaa
     1201 ctttgttttc ccagcagcat ctattccgtc attatttgcc agatcataaa gattgcccga
     1261 agaaagcttt actgatgtca cccgtgtctt taccgtgact ttgcaggaca cagttttttt
     1321 ggtgccgtct gtggtggtgg cttttatggt tacagtaccg ttcttcttgc ccttgacgat
     1381 accatttgat gttactgttg caatgctttt gctgcttgtt tcatatttta cagaccggtt
     1441 tgaggcagat gaaggtgtgc attttgaatt gaagttaaaa ctctttcctt tgtaaatggt
     1501 tttttttgaa gaggcgtttt tccatgacac ttttttcacc ttcttcccga ctgtcacttt
     1561 tacagaatcc ttttttttgg atccgtcctt agcggtggct gttattgttg cggtgccttt
     1621 cttttttgct tttatggtcc cttttgagga tacataaatt acagatg
//
LOCUS       DAONHK010000184         1636 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_69006_length_1636_cov_3.824794, whole genome shotgun sequence.
ACCESSION   DAONHK010000184 DAONHK010000000
VERSION     DAONHK010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69006_length_1636_cov_3.824794"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            86..781
                     /gene="dapB"
                     /locus_tag="PLM92_05795"
     CDS             86..781
                     /gene="dapB"
                     /locus_tag="PLM92_05795"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005884870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HPR24684.1"
                     /translation="MMKIVISGHGRTGSILQEMINENKNMSEATAEDGDFDVIIDFSH
                     PDNLEKIKEYALKYKKPVVIATTGYTKRQELSIEEMSKSVAVVFSPNFSPGITLMQRL
                     ISEITPLLKDSFDMEIIEKHHRNKLDAPSGTANLLLYAADPDNEYCVKHGRFGNGKRE
                     KEIGIHSLRCGSISGEHSVIFAGEDEVIEITHRAVSNRIFAAGALKAALFALEQMPGL
                     YSMHDVLLGGEKK"
     gene            778..1476
                     /gene="dapD"
                     /locus_tag="PLM92_05800"
     CDS             778..1476
                     /gene="dapD"
                     /locus_tag="PLM92_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018133016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HPR24685.1"
                     /translation="MIDAQTVIQRISNSKKKTPVKVILSERADVDFAGCRVFGRGDLK
                     IVYGDWEDIKDVIAENREKISDYVVENNCRNSAIPLMDTKNIKARIEPGAIIREGAQI
                     GEGAVIMMGAVINIGASVGKMTMIDMNAVLGGRARVGRNCHIGAGAVLAGVIEPPSAE
                     PVIIEDDVMIGANAVILEGVKVGKGSVVAAGAIVTENIPEGVVAAGMPARIIKAKDQK
                     SKAKTVMAEELRKI"
BASE COUNT          576 a          272 c          437 g          351 t
ORIGIN      
        1 tgccccatgg aaaaccggac gagggaagtt ctgaaaagca gtatgcagga tctcggaata
       61 atatgaaatg taggggaagg agacaatgat gaaaatcgta atatcaggcc atggaagaac
      121 gggcagcatt cttcaggaaa tgataaatga aaataaaaat atgagtgagg cgacggcaga
      181 agacggagat tttgatgtga taatagattt cagtcatcct gataatctgg aaaaaataaa
      241 agaatatgca ctcaaatata aaaagcctgt ggtcatagcc actaccggat atacaaagag
      301 acaggaactt tccatagaag aaatgtcaaa aagtgtggct gtggtttttt ctcccaactt
      361 ttccccgggg ataacgttga tgcagcgttt gatctcagag atcacccctt tgctcaaaga
      421 tagttttgac atggagatca tagagaaaca ccatagaaac aaactggatg ctcccagcgg
      481 cacagccaat ttgctgcttt atgcagcaga tcctgataat gaatactgtg ttaagcatgg
      541 acgttttgga aacgggaaaa gagaaaagga aataggaata cattctttga gatgcgggag
      601 catttcgggg gaacattcag tcatatttgc aggtgaggat gaggtgatcg aaataaccca
      661 tagagctgtt tccaaccgca tatttgcagc aggggcactt aaagccgctt tgtttgcatt
      721 ggaacagatg ccgggactat attccatgca tgatgtgctt ttgggaggtg aaaaaaaatg
      781 atagatgcac agacggtaat acagcgtata agcaactcca aaaaaaagac tcctgtcaaa
      841 gtcatccttt ccgaaagggc agatgtggat ttcgccggat gcagggtatt cggaagagga
      901 gacctgaaaa tagtatatgg tgactgggag gatatcaagg atgttatagc agagaaccgg
      961 gaaaaaatat ccgattacgt ggtagagaat aactgcagga actcggccat acctttgatg
     1021 gatacaaaaa acataaaagc ccgcatcgaa cctggagcaa tcataagaga gggggcgcaa
     1081 ataggggaag gagctgttat aatgatggga gccgtgataa atataggagc atcggtggga
     1141 aagatgacta tgatagacat gaatgccgta ctgggaggaa gagcccgggt gggcaggaat
     1201 tgccatatag gagcgggcgc ggtgcttgcc ggagtcattg aaccgcccag tgcagaacct
     1261 gtgataatag aagatgatgt tatgattggg gcaaatgctg tgatccttga aggagtaaaa
     1321 gtcggtaaag gttctgttgt ggcggcaggt gccatcgtga cggaaaacat acccgaggga
     1381 gtggtggcag caggtatgcc ggccaggata ataaaagcaa aagaccaaaa atcaaaggcc
     1441 aagaccgtga tggcagaaga actaagaaag atctgataaa atacaaccac aaaaaacagc
     1501 gaggagataa ttattatatg aatagtacgg aaataaagga aagagacaaa aaaaacataa
     1561 taaacaccta taaaagaaat gacacggtaa taatttcagg gaaaaatgct ttgtgctatt
     1621 ctcccgaggg caggga
//
LOCUS       DAONHK010000185         1624 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_69851_length_1624_cov_3.320586, whole genome shotgun sequence.
ACCESSION   DAONHK010000185 DAONHK010000000
VERSION     DAONHK010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69851_length_1624_cov_3.320586"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            75..872
                     /locus_tag="PLM92_05805"
     CDS             75..872
                     /locus_tag="PLM92_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019531665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPR24686.1"
                     /translation="MNRFIDFTDRICLVTGAGNENGIGFQTARILGELGGRVILISTT
                     ERIYKRAEELKKMDTNAEGMTADLMDRTQVKSVIEEIIKKYSRIDVLINNAGMVQVGQ
                     EEEFAAFSEMTDKSWDDSIDRNLNITFNVTRQVLSHMIKNKYGRIVNTSSVTGPLVSN
                     PGEAAYSAAKAAVVGMSRAIAIENAKHNIMINNVLPGWIATDSQTEAEAVGGENTPMG
                     RGGSAEEVANMNVFLASEKASYITGQTFVVDGGNTIQEYKGPKGSYY"
     gene            956..1369
                     /locus_tag="PLM92_05810"
     CDS             956..1369
                     /locus_tag="PLM92_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003542221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3842 family protein"
                     /protein_id="HPR24687.1"
                     /translation="MQIAVVDGQGGGIGRSIVEKLKAELPGTKVVALGTNSVATGQML
                     KAGADDGATGENAIVHNMKHVDVVVGVVGILTANSMMGELTPNMAVAIGSSHTYKVLL
                     PLNRCHIHVVGTEEAPMGKHIDEAVKEVKSYIAGK"
     gene            1423..>1624
                     /locus_tag="PLM92_05815"
     CDS             1423..>1624
                     /locus_tag="PLM92_05815"
                     /inference="COORDINATES: protein motif:HMM:NF025306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4213 domain-containing protein"
                     /protein_id="HPR24688.1"
                     /translation="MFVVKNVLEKSEQFFFDKRVEDIVVGVSLIAVQLSDGSVGVSYV
                     LRNSLPTGCSIFPYAKEVPGKTV"
BASE COUNT          536 a          279 c          432 g          377 t
ORIGIN      
        1 tgtatcacaa agggttggtg cctcattttt cattcttaac tttacagacc gaaaaaagac
       61 tttgggagga agtaatgaat agatttatag attttactga caggatatgt ctggtgaccg
      121 gagcaggcaa cgaaaacggc atcggttttc agactgcaag gatactggga gaactggggg
      181 gaagggtgat ccttatatcc accaccgaaa ggatatataa aagagctgaa gaattgaaaa
      241 aaatggacac caacgctgag gggatgacag cagatctgat ggacaggact caggtaaaaa
      301 gtgtcattga ggagatcata aaaaagtatt cccgaataga tgtgcttata aataatgccg
      361 gcatggtaca ggtgggacag gaagaggaat ttgcagcttt ttcggagatg acagacaaaa
      421 gctgggacga ttccatagac agaaacctta atatcacatt caatgtaacc aggcaggtat
      481 tatcccatat gataaaaaac aaatatggca gaatagtaaa tacttcttct gtgacagggc
      541 ctcttgtcag taacccggga gaagccgcat acagcgctgc aaaagctgct gtagttggaa
      601 tgagcagagc aatagctata gaaaatgcca aacacaacat aatgataaac aatgtgttac
      661 cgggctggat agctacggat tcccagacag aggctgaggc tgtgggagga gaaaacactc
      721 ccatggggcg tggaggaagc gcagaggaag tggcaaatat gaatgtgttc ctggcatcgg
      781 aaaaggcttc ttatataacg gggcagactt ttgttgttga cggcggtaac actattcagg
      841 aatataaagg tcccaaagga tcatattact gacgtatccg ggatgcgtaa taccggtttc
      901 atcttttgga ttttattaca ctgtgatata attgtttatg cggaggtata gacaaatgca
      961 aatagccgtt gttgacggac agggcggagg tataggccgg tccatagtag aaaaattaaa
     1021 ggcagaactg cccggtacaa aagtagtggc actgggcaca aattcggtcg ccacaggaca
     1081 gatgttaaaa gcgggggcag atgacggagc caccggagaa aatgcaatag tgcacaacat
     1141 gaagcatgtt gatgtggtgg tgggggtagt gggaatactt acggccaatt caatgatggg
     1201 agagttgaca ccgaatatgg cagttgccat agggagcagc cacacatata aagtgctgct
     1261 tccccttaac agatgccata tacatgtggt cggaacagaa gaagcaccca tgggaaagca
     1321 tatagacgag gcggtaaaag aagtaaagtc ttatattgca gggaaataaa aattcacctc
     1381 aaaaatcata tctgaacaat atacatagat ggaggaacgg aaatgtttgt ggtaaaaaat
     1441 gtcctggaaa agtcagagca gttttttttc gacaaaaggg tagaagatat agttgtcgga
     1501 gtatctctta ttgctgtaca gctgtccgat ggcagcgtgg gtgtgtcata tgttttgagg
     1561 aatagcctgc ccaccggctg ttctatattt ccatatgcaa aagaagtacc gggcaaaaca
     1621 gtaa
//
LOCUS       DAONHK010000186         1603 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_71350_length_1603_cov_2.433463, whole genome shotgun sequence.
ACCESSION   DAONHK010000186 DAONHK010000000
VERSION     DAONHK010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71350_length_1603_cov_2.433463"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..874)
                     /locus_tag="PLM92_05820"
     CDS             complement(<1..874)
                     /locus_tag="PLM92_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPR24689.1"
                     /translation="MQIKILIVEDSSSDRLIIQKRLSQYETLTACDGMEAMKIIEKNN
                     DISLIILDLKMPVMDGFQVLEKINADERLKKIRTIILTNYDEPDNEIKGLKLGAADYI
                     RKPIQMDSLKARIEVHIELIRAEKALENKLHEQAITFDTIFQKVPVGISIAHNKDKVF
                     SETNRFFSVNPAFERITGRTKEELVEMGWQSITHPEDLQKALENARKLDAGEIEGYSM
                     EKRYIRPDGSTVWVNVIVAPLVLSNDHKYNHIAIVQDITKRKSIEDALTESERSKSVL
                     LSHLPGMAYRCNNDR"
     gene            complement(900..>1603)
                     /locus_tag="PLM92_05825"
     CDS             complement(900..>1603)
                     /locus_tag="PLM92_05825"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPR24690.1"
                     /translation="DDNKTNQLIAKTLLEQMGIEAIIANDGKETLEIYKERKNDIDLI
                     LMDLHMPVMNGYEAAAEIRKISKDVPILALTADVISGVREKCRKNGIYKYISKPLEPE
                     KFMQVIKDVLAEDRNTCVLDADLGLKNMGNDEELYEQVLDIYLTENSNFNEELSSAIY
                     QKKYEDASKMIHKIKSSSGSIGASTLYESAVLLQRALDEENERDIPSLKERFCSLLIK
                     LMGEIGSIREEKNIK"
BASE COUNT          364 a          367 c          282 g          590 t
ORIGIN      
        1 ttctgtcatt gttgcatctg tatgccattc ccgggagatg ggaaaggaga acggatttgc
       61 ttctctcact ttcagtaagg gcatcttcta tactctttct ttttgttatg tcctgtacaa
      121 ttgcaatatg gttatattta tgatcattgg ataaaaccag aggtgccacg atcacattga
      181 cccaaaccgt tgagccgtca ggtctgatat atcttttttc catcgagtat ccttcgatct
      241 ccccggcatc cagttttctg gcattttcaa gcgccttttg aagatcctcc ggatgtgtta
      301 tgctctgcca tcccatctcg acaagctctt cttttgttct gccggtaatt ctttcaaatg
      361 cgggatttac gctgaagaac ctgttcgttt cagaaaaaac cttgtccttg ttatgggcta
      421 tagatatccc caccggcact ttttgaaaaa tcgtatcaaa ggtgatcgcc tgttcgtgca
      481 gtttgttttc gagagctttt tcggcacgta taagctcgat atggacctct atccttgcct
      541 tcagtgaatc catctggata ggttttctga tatagtcagc cgctcccagt ttgagtcctt
      601 tgatctcgtt atcaggctca tcataatttg tgagaattat ggtgcggatc tttttcaggc
      661 gttcatctgc atttattttt tctaatacct gaaatccgtc cattaccggc attttaagat
      721 ccagtatgat caggctgata tcattatttt tctctattat tttcatagcc tccataccgt
      781 cacaggctgt cagtgtttca tactggctga gacgcttttg tataataagt cggtctgatg
      841 aggaatcctc aactatcaaa attttgatct gcatcgtctc ccttcctttc caaaacatac
      901 tactttatgt ttttttcttc cctgatactt cctatttccc ccatcagttt tatcaaaagg
      961 ctgcagaatc tttccttaag agaaggtata tctctttcgt tttcttcatc caaagccctt
     1021 tgcagcaaaa ccgccgattc ataaagggta cttgccccta tgctccccga actgcttttg
     1081 atcttatgga tcattttcga cgcatcttca tatttctttt gatatatggc cgaagaaagt
     1141 tcttcattga aattgctgtt ttcagtcaag tatatatcaa gcacctgttc atagagttct
     1201 tcatcgttcc ccatattttt gagtccgaga tcagcatcaa gcacgcatgt attccggtct
     1261 tcagctaata catcttttat gacctgcatg aatttctcag gttccagagg tttgcttatg
     1321 tatttatata taccgttttt gcggcatttt tcccttacac ctgatatcac atcggcggta
     1381 agagccaata taggcacatc ttttgatatc ttccttattt ccgccgcagc ttcatatccg
     1441 ttcattacgg gcatatgcaa atccataagt atcaagtcga tatcattttt ccgttcttta
     1501 tatatttcaa gggtttcttt tccgtcgttg gcaatgatag cctctatgcc catctgttca
     1561 agcaatgttt ttgctatgag ttgatttgtt ttattgtcat ctg
//
LOCUS       DAONHK010000187         1590 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_72342_length_1590_cov_4.060586, whole genome shotgun sequence.
ACCESSION   DAONHK010000187 DAONHK010000000
VERSION     DAONHK010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72342_length_1590_cov_4.060586"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(52..128)
                     /locus_tag="PLM92_05830"
     tRNA            complement(52..128)
                     /locus_tag="PLM92_05830"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(92..94),aa:Met,seq:cat)
     gene            332..>1590
                     /locus_tag="PLM92_05835"
     CDS             332..>1590
                     /locus_tag="PLM92_05835"
                     /inference="COORDINATES: protein motif:HMM:NF023650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan binding domain-containing protein"
                     /protein_id="HPR24691.1"
                     /translation="MKRSVLIFVCMCAVVLLVSGVFVFAQNHISVSENFPKNTFINGI
                     DCSGLDLEEAEKVLIEEWESREFVIMQDNEEIGNIKNFGFEYDIEDPLNEALLHSAIN
                     PLYTNTIKPQNNLFVPMNISRISDSFKKQFNKLKVLHQKSVTKTENAYVDMSDNDFDI
                     VKEVYGNNIDKDLFFDTIISDIENNVFSLEYIKKDFYEKPTITSENSSLLAKQEYCRQ
                     YLSMEITYDFEDRKNTVPPYALDKMIIVKAGTVSVDRDAVAKYIKSLAAEYDTLGITR
                     EFEGTDGITVEVSGGSYGWLMDQEAEIEWLYRAVKKGKNVLREPEYLQTAFSRSENDI
                     GDSYVEIDLGNQRMWMYKNGQLIVSSSVVTGTPTKNKPTYSGTYRLAYKQPNRVLKGE
                     DWDGTQYESPVKYWMPFNRGQGMHDAP"
BASE COUNT          513 a          323 c          337 g          417 t
ORIGIN      
        1 cagctgagct atgcgcccat attgcttaca caagctattc ttgaaagatt gtggtagcgg
       61 cgagtggagt cgaaccaccg accttccggg tatgaaccgg acgctctaac caactaagct
      121 acaccgcccc accagcgata aaatcatcgc aagagttata ttatcattag aattccactg
      181 tgtcaaggga attaaggtct gtcccaaacc tcatttttgt ttttatatta tacttaaaat
      241 tttgtcacat tccaacattt ttatcagggt cgcttttatt gggtatgtat ttattgtaga
      301 atgatataaa agcaggaaag gatagtttta aatgaagaga agcgtgctca tatttgtgtg
      361 tatgtgtgca gtagttttac ttgtttccgg agtatttgtt tttgcacaaa accatatatc
      421 ggtatcagaa aatttcccaa aaaacacttt cataaacggc atcgactgtt ccggtcttga
      481 tcttgaagaa gccgaaaaag tgctcataga agaatgggaa agcagagaat tcgttattat
      541 gcaggataac gaagagatcg gtaacatcaa aaacttcggt ttcgaatatg atatcgaaga
      601 tcctcttaac gaggcccttt tgcacagtgc aataaaccct ctttatacta acacaataaa
      661 accacagaat aatctttttg tgcccatgaa tatcagccgg atctccgatt cattcaaaaa
      721 acagttcaac aaactcaaag tgctccatca gaagagtgtt accaaaacag aaaatgccta
      781 tgttgatatg tcagacaatg attttgatat agtcaaagaa gtttacggca ataatataga
      841 caaagactta ttttttgata cgatcatttc agatatcgag aataatgttt tttctcttga
      901 atacataaaa aaagatttct atgagaaacc caccattacc tccgaaaact cttctctttt
      961 ggcaaaacag gaatattgcc gccagtatct gtccatggag atcacctatg atttcgaaga
     1021 cagaaaaaat actgtgcccc cttacgctct tgacaaaatg atcatcgtaa aggctggaac
     1081 agtttccgtt gacagggatg ctgtggcaaa atacatcaaa tctctggcag cagagtatga
     1141 taccctgggt atcaccagag agtttgaagg aactgacggg atcaccgtgg aagtatccgg
     1201 cggaagttac ggatggctca tggatcagga agccgaaata gaatggcttt acagggccgt
     1261 aaaaaaaggc aagaacgttc tgcgggagcc tgaatatctt cagactgctt tcagcaggag
     1321 cgaaaacgat atcggagatt cgtatgtgga gatagacctg ggcaatcagc gcatgtggat
     1381 gtataaaaac ggtcagctta tagtaagcag ctctgtggtc acgggcactc ccacaaaaaa
     1441 taaaccgacc tacagcggga cctacaggct tgcctacaag cagccaaaca gagtccttaa
     1501 aggagaagac tgggacggaa cccagtatga aagtcccgtc aaatactgga tgccattcaa
     1561 ccggggtcag ggcatgcatg atgctccctg
//
LOCUS       DAONHK010000188         1584 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_72757_length_1584_cov_5.786135, whole genome shotgun sequence.
ACCESSION   DAONHK010000188 DAONHK010000000
VERSION     DAONHK010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1584)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_72757_length_1584_cov_5.786135"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..567)
                     /locus_tag="PLM92_05840"
     CDS             complement(<1..567)
                     /locus_tag="PLM92_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008513843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HPR24692.1"
                     /translation="MEKNIGFLERKDIEISVKRYVQDALSAMALGLFASLLIGLIIKT
                     IGERVGMLCGVAQTDLETHVFFGFLITVGTFAMSMMGPAIGVAVAWALKSPPLVLFAS
                     AATGYLGATMTGFGIEMAGGPAGAFVAAALGAEFGKMVSKETKVDILVTPAVTIIVGG
                     LAAKFIGPAVGWLMIKLGEVIMTATEWQP"
     gene            760..1275
                     /locus_tag="PLM92_05845"
     CDS             760..1275
                     /locus_tag="PLM92_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013710800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HPR24693.1"
                     /translation="MDPEKRRNRIIEILEKSTEPQSASAIADMLSFSRQVIVGDVALL
                     RASGFDIEATPRGYVLDPSGLSQFPYVGTVACKHYHDRLKEELYAVVDLGGTVIDVTI
                     DHALYGQLSGLLDISSRRDVDLFCEKVEKDGSQPLSILTDGIHIHRIGCKDEETFKTI
                     EKELKDSGIGF"
     gene            complement(1416..>1584)
                     /locus_tag="PLM92_05850"
     CDS             complement(1416..>1584)
                     /locus_tag="PLM92_05850"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003406540.1"
                     /note="catalyzes the formation of succinate semialdehyde
                     and glutamate from 4-aminobutanoate and 2-oxoglutarate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HPR24694.1"
                     /translation="EIVGKIVAGAVQKGLMLEGAGTYNNVIRFLCPLCVTDEQLEKGL
                     EIYEETLKECI"
BASE COUNT          424 a          366 c          315 g          479 t
ORIGIN      
        1 aggctgccat tcagtggctg tcattattac ttctcccagc tttatcatca gccaacccac
       61 tgccgggccg atgaatttcg ccgcaagtcc tccaacaatt atggtaactg caggagtgac
      121 cagaatatcc accttcgttt cctttgaaac catcttgccg aattctgcac cgagggctgc
      181 tgcaacaaag gctcccgcag gacctcctgc catttcgata ccgaatcctg tcattgtggc
      241 tcccagatag ccggtggcag cactggcaaa caaaaccagg ggaggacttt ttagtgccca
      301 tgccaccgcc acacctatgg ccggacccat catgctcatg gcgaaggtgc ccactgtgat
      361 cagaaaacca aaaaaaacat gggtttcaag atcggtctgg gccaccccgc aaagcatacc
      421 gactctttca cctatggttt tgattatcag tcctataaga agtgatgcga aaagccccag
      481 tgccatggca gataaggcat cctggacata tcttttcaca cttatttcta tgtctttcct
      541 ttcaagaaat cctatgtttt tttccatgtt ttttcctttc tttttgtcac aggtatttaa
      601 atttttgcga tgctcttttt gaaatatgac tttctcattg tagcaaagga gaagtgttgt
      661 gtcaagacac ctgtcattac aactttttca tctgctcatt gagttatttt ttataaactg
      721 ttataatcat atatacaaaa agcttaagga gttgttttga tggatcccga aaaaagacgc
      781 aacagaataa ttgagatcct ggagaaaagt actgaaccgc aaagtgcatc ggcaattgcc
      841 gatatgctat ctttcagcag acaggtgatc gtgggcgatg tggctctcct tagagcttcc
      901 ggatttgata tagaagcgac ccccagagga tatgtgcttg acccttcagg tttgtctcag
      961 ttcccttatg tgggcactgt tgcatgcaaa cattaccatg acagactcaa ggaggagcta
     1021 tatgccgttg ttgacctggg cggcactgtt atagacgtaa ctatagacca tgctctttac
     1081 ggtcagcttt ccggtcttct tgacatttca tcaagaaggg atgtggatct tttttgtgaa
     1141 aaagtggaaa aggacggttc tcaaccatta agtatactta ctgacggcat tcacattcac
     1201 cgcataggct gcaaggatga ggaaacattc aaaacaatag aaaaagaact gaaggattca
     1261 ggcataggtt tttaacaaat gtattaatat ttttccacag tataccgcct taaaaaagga
     1321 ccggttcccc attatgaggt ccggcccttt ttattgattt tttgctatgt tccgatgatt
     1381 actgataagt aagtgactct tatgctatct tgattctata tgcactcttt taaagtctct
     1441 tcatatatct caagaccttt ttcaagctgt tcatctgtta cacaaagagg acagaggaat
     1501 cttattacat tattatatgt tccggcgcct tccagcataa gtcccttctg aactgctcct
     1561 gccactatct tgcctactat ttct
//
LOCUS       DAONHK010000189         1521 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_77667_length_1521_cov_2.844475, whole genome shotgun sequence.
ACCESSION   DAONHK010000189 DAONHK010000000
VERSION     DAONHK010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77667_length_1521_cov_2.844475"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..995)
                     /gene="gpmI"
                     /locus_tag="PLM92_05855"
     CDS             complement(<1..995)
                     /gene="gpmI"
                     /locus_tag="PLM92_05855"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005999577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HPR24695.1"
                     /translation="MKTFKKPTVLLIMDGYGISKDGNKIKKGNAIATAKKPHLDRIFS
                     EYPNTTIGASGKDVGLPDGQMGNSEVGHLNIGAGRIVFQDLSRITNEISSKNFFSNKA
                     LTDAMDHCIKNDSSLHLMGLVSDGGVHSHINHLFALIDMAKKKGLKKVFVHCFLDGRD
                     VPPRCAKKYMDQLEEYMKKTGIGRIATLCGRYYAMDRDKRWDRLEKAYNAISIAEGHH
                     APSAADAINQAYKRGEDDEFILPTVIDTPSLLSRDDSVIMFNFRPDRAREITRAFVDP
                     GFDAFKRKKEFNGIYYVCMTRYDASMPAVSVAYPPEEIRNTLGEYLSSKGLKQLR"
     gene            1076..>1521
                     /locus_tag="PLM92_05860"
     CDS             1076..>1521
                     /locus_tag="PLM92_05860"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HPR24696.1"
                     /translation="MKWTPEQKKAIELRNTSILVSAAAGSGKTAVLVARIEHIITGAD
                     ISEEIQYKPVPIDRMLVVTFTNAAASEMKEKISAALNNAALESPKNLSLIQNQLYALQ
                     NANISTFHSFAMEVVRRYFHLTDIDPDFKICDEVESTIMKSEAMDKV"
BASE COUNT          418 a          323 c          315 g          465 t
ORIGIN      
        1 attctcagct gtttaaggcc ttttgaggaa agatactctc ccagagtgtt cctgatttct
       61 tcgggaggat atgctacgct gacagcaggc attgaagcat cgtacctggt catacatacg
      121 tagtatatac cgttgaattc tttttttctt ttgaatgcgt caaatcccgg atccacaaaa
      181 gcccttgtga tctccctggc ccggtcgggt ctgaagttga acataattac agaatcatcc
      241 cggcttaaca aagaaggggt atctattact gttggcagaa tgaattcatc atcttctcct
      301 cttttgtagg cttggtttat ggcatctgcg gcagaaggcg catggtgtcc ttcagcaatg
      361 gaaatcgcat tatatgcttt ttccagtctg tcccaccgtt tgtctctgtc catggcataa
      421 tacctgccgc aaagggtggc tatacgtccg ataccggttt ttttcatgta ttcttccagc
      481 tggtccatgt atttttttgc acatctgggg gggacatctc tgccgtccag aaagcaatgc
      541 acaaagactt ttttcagacc tttctttttg gccatgtcaa tgagagcaaa aagatggttg
      601 atatggctgt ggaccccacc atcagaaaca agtcccatca ggtgaaggga tgaatcgttt
      661 tttatgcagt gatccatggc atctgtaaga gccttattgg aaaaaaagtt ttttgatgat
      721 atttcatttg ttattcttga gagatcctga aagactatcc tgccggcacc tatattaaga
      781 tgaccgactt cagagtttcc catttgtccg tcaggcaacc ccacatcctt acctgatgca
      841 ccaattgtag tgtttggata ctcggaaaag atcctgtcca agtggggttt ttttgctgtg
      901 gcaatagcat tgcctttttt tattttgttg ccgtctttac ttattccgta tccgtccatt
      961 attagcagta ctgttggttt tttgaatgtt ttcatcgcat ccctctgtgt tcttttaagg
     1021 tacatatcgt atgttaatta taccacagag aaaccattca tgatataata gaatcatgaa
     1081 atggacacca gaacaaaaaa aagcgataga gctcagaaac accagtatac ttgtttccgc
     1141 agcagcaggc tcgggtaaga ccgcagttct tgttgcacgt atagagcaca taataaccgg
     1201 tgcagatata tctgaagaga tccaatataa acctgttccc atagacagga tgcttgtagt
     1261 tacattcaca aatgcggcgg cttccgaaat gaaagagaaa atatctgcag ctcttaataa
     1321 cgcagccctc gaaagtccga aaaacctttc tttgatccaa aaccagcttt acgcgctaca
     1381 gaacgcaaac ataagcacat ttcattcttt tgccatggaa gtggtaagaa gatattttca
     1441 tcttacggat attgatccgg atttcaaaat atgtgatgaa gtggaaagca caataatgaa
     1501 atcagaggca atggacaaag t
//
LOCUS       DAONHK010000190         1458 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_83355_length_1458_cov_1.771205, whole genome shotgun sequence.
ACCESSION   DAONHK010000190 DAONHK010000000
VERSION     DAONHK010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1458
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83355_length_1458_cov_1.771205"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="PLM92_05865"
     CDS             complement(<1..345)
                     /locus_tag="PLM92_05865"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPR24697.1"
                     /translation="MKKNSTDICIIGCGAAGMAAAISAAQKSPAKDIFIIEKKEMPGK
                     KLYATGNGRCNISNRECMDSDDVMSFFGSVGISIREEEEGRLYPLSGQASDVVLALEK
                     AMEVNNINIIVNS"
     gene            464..1303
                     /locus_tag="PLM92_05870"
     CDS             464..1303
                     /locus_tag="PLM92_05870"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HPR24698.1"
                     /translation="MRSIMKFFFVFIIIVLIAVLIGAGILAYSFFVEPQRLSETELKM
                     SDPKITKSLSVAVFADTHFGNDYDISDFKKATEKINEASPDIIIFLGDLIDDYNNYDG
                     DMDHIISALSSLDAPYGKYAIFGNHDYGGGAERLYKGWMASAGFTVLKNSTVSLKGNI
                     ALTGIDDCLIGYGDPSAANGLSPDHYNIVICHEPDIADSIAGTNTDLMIAGHTHGGQV
                     NIPFYKDRYLPDLGKKYIKGKYILDNKEKTVVYVNNGLGTTKVGARFMAVPEVTYFFL
                     CGI"
     gene            complement(1279..>1458)
                     /locus_tag="PLM92_05875"
     CDS             complement(1279..>1458)
                     /locus_tag="PLM92_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPR24699.1"
                     /translation="KKNHYWAMGTGIICVVAGAYAFLDYLLLGMGTVMLLGIIFVLQG
                     ISTLELGIHMPHKKK"
BASE COUNT          428 a          317 c          271 g          442 t
ORIGIN      
        1 gctatttaca ataatattta tattgttaac ttccatagcc ttttctaaag caaggaccac
       61 atcagacgcc tggccggata aaggatacaa ccgcccttct tcttcctctc tgatggatat
      121 tccaacagat ccaaaaaaag acatcacatc atcagagtcc atacattccc tgtttgaaat
      181 gttgcatctc ccattgcctg tggcatacag ttttttgccc ggcatctctt ttttttctat
      241 aatgaatata tcttttgccg gcgatttttg tgcggcagaa atcgccgcag ccatgcctgc
      301 ggcaccacag ccgattatgc agatgtcagt actattcttt ttcataacat atcgattgta
      361 tcacaatttt tttccgatct ctacaaactc ctgaaactca ttatctcatc attgatttgc
      421 ttgtgtttca atgttatcat atttcagtca ggaggtttac agattgagat caataatgaa
      481 attctttttt gtttttataa taatagtttt gattgccgtt cttataggtg ccggcatttt
      541 ggcttattct ttctttgtgg aaccgcaaag actatcggag acagaactta aaatgagcga
      601 tcctaaaata acgaagtcgc tgtccgttgc agtttttgct gatacccatt tcggaaatga
      661 ttatgatata tccgacttca aaaaagccac agagaaaatc aatgaagctt ctccggacat
      721 catcattttt ttgggcgatc ttatcgatga ttacaacaac tatgacggtg atatggacca
      781 tatcatctca gctctttcat ctcttgatgc cccctatggc aagtatgcca tattcggaaa
      841 tcatgattac ggaggagggg cagaaaggct gtataaggga tggatggcgt ctgcaggctt
      901 tacagtgtta aaaaacagca ctgtaagcct aaagggcaat attgccctta caggcataga
      961 tgactgtctc ataggatacg gagatccttc tgcggcaaac ggcctttcac cggatcatta
     1021 taatatcgtt atctgtcatg aacccgatat tgctgacagt atagcaggca cgaacacaga
     1081 tctgatgata gccggccata cccacggcgg tcaggtcaat ataccttttt ataaggaccg
     1141 ttatcttcct gatctgggca aaaaatatat aaaagggaaa tatattctgg ataataaaga
     1201 aaagaccgtc gtatatgtta acaacggtct gggaacaaca aaagtcggtg ccagatttat
     1261 ggccgtcccc gaggtgacct atttcttttt atgcggcata tgaataccca actctaaagt
     1321 acttattccc tgaagaacaa atattatacc aagcagcatt actgtgccca ttccaagtag
     1381 aagataatca agaaaagcat aagctcccgc aacaacgcat attatacccg tacccattgc
     1441 ccagtaatga ttcttttt
//
LOCUS       DAONHK010000191         1428 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_86044_length_1428_cov_3.249818, whole genome shotgun sequence.
ACCESSION   DAONHK010000191 DAONHK010000000
VERSION     DAONHK010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1428
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86044_length_1428_cov_3.249818"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="PLM92_05880"
     CDS             complement(<1..210)
                     /locus_tag="PLM92_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009535662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HPR24700.1"
                     /translation="MSRMHQRTETITGTEATAALKRAKVAVFGIGGVGGYAAEALARA
                     GIGTIDLIDRDRIEESNLNRQIIALR"
     gene            complement(228..686)
                     /locus_tag="PLM92_05885"
     CDS             complement(228..686)
                     /locus_tag="PLM92_05885"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HPR24701.1"
                     /translation="MDINDVALRHLERRSRTKKEIKDHLAEKGFSDEDIRDCISYLEN
                     SFYLDDQRYCGEYLRYSFAKGWGKKKIAANLARLGISGEDIEEASRSYEEENGVDLSE
                     EEIKRALRQAEKLATASGKEENLPAKLGRKLAYLGYDTETVYKVVGKYMK"
     gene            complement(676..>1428)
                     /locus_tag="PLM92_05890"
     CDS             complement(676..>1428)
                     /locus_tag="PLM92_05890"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPR24702.1"
                     /translation="ETYGYNILFDTGATAAVFSQNAKRMSVDLKSVDLAVISHGHYDH
                     AGGVPEFCKINKKAPVFIHKDSFPDSYGTDENGQIDKEPCGILWTDEEKKKVKDRLVL
                     TDGPTWLTDNVCISGTIPDYKGMAMTEDFYIKCQDGTYVKDNMTHEQFLAIREEDLFI
                     FSGCSHKGVIPVIKYAKELFPKEKITLLAAGMHLYSASADMRKKVIKQVIKEKVDNIM
                     PVHCTGIEAICELKTAMGENCIIATAGDSYGY"
BASE COUNT          312 a          354 c          266 g          496 t
ORIGIN      
        1 tcttaaggct atgatttgtc tgttaagatt tgattcctct atccggtccc tgtctatcag
       61 gtcaatggtc cctatcccgg ctcttgccag agcctctgcc gcgtagcctc ccaccccccc
      121 gataccgaaa acagccacct tcgccctttt tagagctgct gtggcttcag ttccggttat
      181 agtttctgtt ctttgatgca ttctgctcat aattttatga tttccgttca cttcatgtat
      241 ttaccgacta ctttgtaaac cgtttctgtg tcatatccca gataggcaag tttccttcca
      301 agttttgccg gcaaattttc ttcctttcct gatgctgtcg ccagcttttc tgcctgacgg
      361 agggctcttt ttatttcctc ttctgaaaga tctactccgt tttcttcttc atagcttctt
      421 gatgcttctt caatatcctc ccctgaaatg ccaagccttg ccagatttgc agcgattttt
      481 ttctttcccc atccctttgc aaaagaatac cgtaaatatt cgccgcagta tctttgatca
      541 tcaaggtaaa aggaattttc cagataagat atgcaatccc taatatcttc gtctgagaat
      601 cccttctctg ccagatgatc tttaatctct ttttttgtcc ggcttcttct ttcaaggtgt
      661 ctgagagcaa catcgttaat atccataact gtctcctgca gtggctatta tgcagttttc
      721 tcccatggcg gtcttgagct cacagatagc ctcaatgcct gtacaatgca ccggcataat
      781 attgtcgacc ttttccttaa tgacctgttt gatgaccttc tttctcatat cggcagaagc
      841 gctgtaaaga tgcatgcctg ccgccagaag agttattttt tcttttggaa aaagttcctt
      901 tgcatatttt ataacaggga tgactccttt atggctgcat cccgaaaata tgaaaagatc
      961 ttcttccctt atggccagga actgttcatg ggtcatatta tcttttacat aggtgccgtc
     1021 ctggcatttt atataaaaat cctcagtcat ggccatgcct ttgtaatcgg gtatggtgcc
     1081 tgaaatgcat acattgtcgg tcagccatgt gggtccgtca gtgagcacaa gtctgtcttt
     1141 tacttttttc ttttcttcat cggtccaaag aataccgcag ggttccttat ctatctggcc
     1201 gttttcatcg gtcccgtaag agtcgggaaa agaatcttta tgtatgaaaa cgggagcttt
     1261 tttgtttatt ttacaaaatt ccgggacacc tcccgcatgg tcatagtggc cgtggcttat
     1321 aacggcaaga tccacgcttt ttagatcaac ggacattctc tttgcgtttt gcgaaaacac
     1381 tgctgccgtg gctcccgtat caaaaagtat gttataccca tatgtctc
//
LOCUS       DAONHK010000192         1402 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_88645_length_1402_cov_2.992576, whole genome shotgun sequence.
ACCESSION   DAONHK010000192 DAONHK010000000
VERSION     DAONHK010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1402
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_88645_length_1402_cov_2.992576"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..784
                     /locus_tag="PLM92_05895"
     CDS             <1..784
                     /locus_tag="PLM92_05895"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HPR24703.1"
                     /translation="IVEIICVEKGRLKISDCALDHTLDPGDVYIFNAMDAHRIRPLSP
                     GTVILTVHIDKRYYSRFFEDLRDIYFICGSESPEKDSRELDYLKHLMHALFKECSSET
                     PSDLVSENLVREMLELLCEQFRYYFYRKEPDGSYMLFRRSSSTAAMSHNKRAYEIVDY
                     IYDNFFCRLRLTDVAAKYYLSPSHMSRYIKKVIGLSFSEILTLARCEEAERLLFDTEK
                     TVDEIAGEVGFVNRKHMAVNFRKWYGKTPTNWRKSLEEEKIF"
     gene            complement(910..>1402)
                     /locus_tag="PLM92_05900"
     CDS             complement(910..>1402)
                     /locus_tag="PLM92_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008705597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HPR24704.1"
                     /translation="KVAEEGEKGVKEVTSIVKKENGEVIDSKVIGEKVTKKPEAKVVI
                     KGTKISKTGAKVASYALQFVGNPYVWGGTSLTNGADCSGFTLSVYSHFGISLPHSAAA
                     QRSYGKAVSYGDARPGDLFFYPHHVAIYIGGGQIVHASTSSTGIIVGNAGYRPLSCIR
                     RLL"
BASE COUNT          362 a          310 c          303 g          427 t
ORIGIN      
        1 cattgtagaa ataatctgtg tggaaaaagg ccgtctcaag atctccgact gtgctctgga
       61 tcacaccctt gatccggggg atgtctatat ttttaatgca atggacgctc acaggatacg
      121 ccctttatca cccggcaccg tgattttgac agttcatatc gacaagcgct attacagcag
      181 gttttttgaa gacctgcggg acatttattt catatgcgga tctgaaagtc ccgaaaaaga
      241 ttcccgggaa cttgattacc ttaaacatct aatgcatgcc ctgttcaaag agtgctcttc
      301 agaaactccc agtgatcttg tatcagaaaa cctggtaagg gaaatgctgg agcttctttg
      361 cgaacagttt cgctattact tttacagaaa agagcccgac ggcagttata tgctttttcg
      421 cagatccagc tctacggcag ccatgagcca taacaaaaga gcttatgaaa tagttgacta
      481 tatctatgat aactttttct gccgactgag gctgactgac gtggcagcaa agtattactt
      541 gagtccctct cacatgagca gatacataaa aaaagtgatc ggtctctctt tttcggagat
      601 actgactctc gcccgttgcg aagaagccga acggcttctt tttgatacgg aaaaaaccgt
      661 ggatgaaata gcgggagaag tgggctttgt caacagaaag catatggcgg tgaatttcag
      721 aaaatggtat ggaaaaactc cgacaaattg gcgcaaaagc ctggaagaag aaaaaatatt
      781 ttagcacaat aaaaaagagc tgcccggcaa aatcacttgc actgtgggca gctcattttt
      841 gttttgattt tttgttttgg cctttttgat attgctcttt tttcttggcc tttttggttt
      901 tgcacttttt tacaaaagac gtctgatgca tgagagcggt ctgtaaccgg catttccaac
      961 tattattcct gtactggaag tactggcatg tactatctgc ccgccgccga tatatatggc
     1021 tacatggtgc ggataaaaga acagatctcc gggtcttgca tcgccgtatg aaacagcctt
     1081 tccgtagctc ctctgggcgg ctgcactgtg aggcagtgaa atgccgaaat gtgaataaac
     1141 actcagagtg aatccggagc agtcagctcc attggtaaga cttgttcctc cccatacata
     1201 gggattccct acaaactgaa gtgcgtagct tgcaactttt gctccggtct tgctgatctt
     1261 ggttcctttt attacgactt ttgcctcggg cttttttgtt actttttctc cgatgacttt
     1321 gctgtctatt acttctccgt tttctttttt tactatggaa gtaacttctt taacgccctt
     1381 ctcaccttct tcagcgacct tt
//
LOCUS       DAONHK010000193         1395 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_89343_length_1395_cov_4.119403, whole genome shotgun sequence.
ACCESSION   DAONHK010000193 DAONHK010000000
VERSION     DAONHK010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1395)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1395)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1395
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89343_length_1395_cov_4.119403"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1395
                     /locus_tag="PLM92_05905"
     CDS             <1..>1395
                     /locus_tag="PLM92_05905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HPR24705.1"
                     /translation="KIDRELFDEFETDEKVAGILASVMRMARWLSISVITEGVESENQ
                     RQFLKNIGCNMMQGYYFLRPVPADKFMEYVKKNKKKTAKGAGAGALTFDAGDILDNDV
                     MMNMIYHKFMEGVGIYELIGDKLKIIRVNDGYCRMFGYDPKSTPEGADNIYLMIHEDY
                     RDMLKEECQTIAREKCERQFELRRKTGYGKYVWVWAGGTYIGMKNDHPVICFLLRDIT
                     RYVESQKQAEIKVAKQRELYMKLMEDTNVGIFEIETEKDELTVYTKMPDGQRKDFTIK
                     GIASEQGDLSSDNIIMKKIFPGNKNIYKEVINAAEKGQQNGEIEYKADYYGDGYKWYK
                     SYYETVLDQEKGKVTNIIGRTVSIEKEKEIEHRLKQEKAYRAAIFKEMLVVYEMDITL
                     DISVPMYRNQKRAGEYYPFNYYTDFEKSRDYIYSEDYEDVKESFQEAYSGKLLEKGIE
                     DVKKKYRIKNRKGD"
BASE COUNT          534 a          215 c          345 g          301 t
ORIGIN      
        1 tgaagataga cagggaactt tttgatgagt ttgaaacaga cgaaaaggtc gcaggcatac
       61 tggcctctgt gatgcgaatg gccaggtggc tttcaataag cgtaataaca gaaggtgtgg
      121 aaagcgaaaa ccagagacag ttccttaaaa atatcggatg caacatgatg cagggatatt
      181 attttctcag gccggtcccg gctgataaat tcatggaata tgtgaaaaag aataaaaaga
      241 aaacggcaaa aggcgccgga gccggggcgc ttacctttga tgcaggagat atacttgaca
      301 atgatgtgat gatgaacatg atatatcata aattcatgga gggggttgga atatatgaat
      361 tgatcggcga caaactgaaa ataataagag tcaatgacgg ctattgcagg atgttcggct
      421 atgacccgaa atcaacgcca gaaggagccg acaatatcta ccttatgatc catgaggact
      481 acagggacat gctcaaggag gaatgtcaga cgatagccag ggaaaaatgt gaaagacagt
      541 ttgaattgcg caggaagacc ggatatggca aatatgtttg ggtctgggcc ggaggcactt
      601 atataggaat gaaaaacgac catcccgtta tttgcttttt gctccgtgat ataacaagat
      661 atgttgaaag ccagaaacag gcagaaataa aagtcgcaaa acagagagag ctgtatatga
      721 agcttatgga agataccaat gttggcatat tcgagataga aacagaaaaa gatgagctta
      781 cggtatacac aaaaatgcct gacggccaac gaaaggattt tacaattaaa gggatagcaa
      841 gtgagcaggg tgatctaagc agcgataata taataatgaa aaagatattc cccggaaaca
      901 aaaacattta taaggaagta ataaatgctg cagaaaaagg gcagcagaac ggagaaatcg
      961 agtacaaggc tgattactac ggggacggat acaaatggta taaatcatat tatgagactg
     1021 ttcttgatca ggaaaagggg aaggtcacca atataatagg cagaactgtc agtatagaaa
     1081 aggaaaagga aatagaacac agactgaaac aggaaaaagc ttacagggcc gctattttca
     1141 aagagatgct ggtggtatac gagatggata taacccttga cataagtgtt cccatgtaca
     1201 gaaaccaaaa aagagcaggc gaatactatc cgttcaatta ctataccgat tttgaaaaaa
     1261 gcagagatta tatctatagt gaagactatg aagatgtaaa agaaagcttt caggaagcat
     1321 attccgggaa acttcttgag aaaggtatcg aagatgtaaa gaaaaaatac cgtataaaaa
     1381 acagaaaagg cgact
//
LOCUS       DAONHK010000194         1385 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_90399_length_1385_cov_3.706767, whole genome shotgun sequence.
ACCESSION   DAONHK010000194 DAONHK010000000
VERSION     DAONHK010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1385
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_90399_length_1385_cov_3.706767"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            80..553
                     /locus_tag="PLM92_05910"
     CDS             80..553
                     /locus_tag="PLM92_05910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPR24706.1"
                     /translation="MTEKQKLIDRINEFYFEIIEEYKEITAKIKAESSATAVFKKKDY
                     EGNINRLKKCKAAALRIDVKNVKIDEDDETSKDVVERFERALSLFNSLCDSQIQLQTA
                     FMQKAKREAKVTFGDCSVIMKKVNTNNGATQVAIHDLDLVFADYIADEEYEPRGC"
     gene            582..1325
                     /locus_tag="PLM92_05915"
     CDS             582..1325
                     /locus_tag="PLM92_05915"
                     /inference="COORDINATES: protein motif:HMM:NF016051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HPR24707.1"
                     /translation="MKNILITGGPTNEPIDEVMKITNMSTGSLSVSLGQLFYEAGYKV
                     CLVLNNSVSADKLPIDKNISVIRIETTDDMSNAIETKSRQEHFDAVIHAAAVGDYKAD
                     FSFLMEDMAAEIFRVKDTIESEKEILDIMLDPVCKIDDSSKISSYQANLTVKLGLTQK
                     IIAKLREWFPGALLIGCKLLENVQEEELFQVAQALCKKNRMDYILANDLAKLRAGITA
                     RYPVSNEGYTGHKLDTAKDIFDFVNEKLG"
BASE COUNT          503 a          260 c          309 g          313 t
ORIGIN      
        1 ttcgtcactt ttggcaatga ggacgaaaaa ctttacagat atatactaaa tcaatataat
       61 tgacaaggga ggaaacaata tgacagaaaa acaaaaactt attgacagga tcaatgaatt
      121 ctactttgag ataatcgaag aatacaaaga aataacagca aaaataaaag cagagtcttc
      181 tgctacggct gtattcaaaa aaaaggatta tgaggggaac ataaacaggc taaaaaaatg
      241 caaagcggca gccttgagga tcgatgtaaa aaatgtaaaa atagacgaag acgatgaaac
      301 ctcaaaggat gtggtagaga gattcgaaag ggcactttcc ctttttaact ctctttgtga
      361 ttcccagata cagcttcaga ccgcgttcat gcaaaaggca aaaagagagg caaaagtcac
      421 cttcggcgac tgcagtgtca taatgaaaaa agtcaacaca aacaatggcg ccactcaggt
      481 ggccatccat gatcttgatc tggtattcgc agattatatt gcggatgaag aatatgaacc
      541 ccgcggatgc tgacaaatac attttattaa ggagaaatgg aatgaaaaat atactcatta
      601 ccggaggccc taccaacgaa cccattgacg aagtcatgaa aatcaccaat atgtctacag
      661 gaagcctttc cgtctccctg ggacagcttt tttatgaagc aggatacaag gtgtgtcttg
      721 tgctcaacaa cagcgtatcc gccgataaac tgcccataga caagaatata tccgtaataa
      781 ggatagaaac cacggatgac atgtcaaatg ccattgaaac aaagagcagg caggagcatt
      841 ttgatgcagt catacatgcg gcggcagtgg gtgattacaa ggcggatttt tcatttctta
      901 tggaagacat ggcagccgag atattcagag tcaaagatac tatagagtcg gaaaaggaga
      961 tactggatat aatgctggac cctgtatgta aaatcgatga ttcttccaag ataagcagct
     1021 atcaggccaa ccttacagta aaactggggt tgacccagaa gatcatcgca aaattgaggg
     1081 aatggttccc cggagctctc cttataggat gcaagctgct ggaaaatgta caggaagaag
     1141 agcttttcca ggttgcccag gctctgtgca aaaagaaccg aatggattat atacttgcca
     1201 atgatctggc aaaactaagg gccggcatta ctgccagata tcccgtatca aatgaagggt
     1261 acaccggtca caaactggat acggcaaagg atatatttga ttttgtcaat gagaagctcg
     1321 gctgataaaa aattataaat ggacatccat acgggagaaa gaatcaaaac caataacggg
     1381 agaaa
//
LOCUS       DAONHK010000195         1224 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_110492_length_1224_cov_5.028229, whole genome shotgun
            sequence.
ACCESSION   DAONHK010000195 DAONHK010000000
VERSION     DAONHK010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1224
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110492_length_1224_cov_5.028229"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="PLM92_05920"
     CDS             complement(<1..233)
                     /locus_tag="PLM92_05920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPR24708.1"
                     /translation="MDEKAAKSIEKWEAEKKSLESVKRYIKKYQKLKEDKENDNKAEM
                     NKIGEKVTAIFEDSKWMKLEWKEIKKSKKALEEG"
     gene            complement(346..>1224)
                     /locus_tag="PLM92_05925"
     CDS             complement(346..>1224)
                     /locus_tag="PLM92_05925"
                     /inference="COORDINATES: protein motif:HMM:NF012703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HPR24709.1"
                     /translation="QAKVCMMGIPYDGGSLLGKGAVEAPMKIRDLARRFMPEGTDSWC
                     PIEKNDLYDFGDLIVDETYAATYVNAENAAYEVMKQGKLSVFVGGDHSMTIPIHKSYA
                     KYQREKDPDAKIGIIHFDAHLDMAESFEGRHWSHSSTARHALNDVIKPEDLFFVGVRA
                     AEKEELDMLKENPEIGMISAMQIFTRGYPNAFKEIAMRYKKYDSIYLTIDIDVLDPAS
                     APGTGTPAPGGITTQGLIYLVEKIMEILPVDAMDIMEISPPLDINDITSWAGMRLLQE
                     AIASTKEAVYMDTKKV"
BASE COUNT          270 a          291 c          205 g          458 t
ORIGIN      
        1 ccttcctcaa gggctttttt gctttttttg atttctttcc attccagctt catccacttg
       61 ctgtcttcaa aaattgctgt tactttttca ccgatcttat tcatttcggc tttgttgtca
      121 ttttccttat cttccttgag tttctgatac ttttttatgt atcttttcac gctctccagg
      181 gatttttttt ctgcttccca tttttctatg ctctttgcag ctttttcatc cattttaaaa
      241 agtccctccc tctttgtcac aaattagact tattgccatt atattaaaca gatttttttt
      301 gtcaaccgaa attgagttat tattctgttt tcgcctttta tttgtctaaa cttttttggt
      361 atccatatat acggcttctt ttgtggatgc tatggcttcc tgcagaagtc tcatccctgc
      421 ccaggaagtt atatcgttga tatcaagagg cggagatatt tccattatat ccatggcatc
      481 tacaggcagt atttccatta tcttttctac aagataaatg agaccctgcg tggttatccc
      541 tccgggagcg ggagtcccgg tccccggtgc tgatgcggga tccagcacat ctatatcaat
      601 agtaagatat atagaatcat attttttgta tctcattgca atttctttga aagcattggg
      661 atatcccctt gtaaatatct gcatcgcaga tatcattccg atctcggggt tctcttttag
      721 catatccagt tcttcttttt ctgcggcacg tacaccaaca aaaaacaggt cttcgggttt
      781 tatgacatcg tttaacgcat gtctcgcagt gctggaatgt gaccagtgtc ttccttcaaa
      841 actctctgcc atatcgagat gggcatcaaa atgtattatt cctatcttcg catccggatc
      901 tttttctctc tggtattttg catacgactt atggataggg atggtcatgg agtgatcgcc
      961 gccgacaaaa acacttaatt tgccctgctt catcacttca tatgctgcat tttcagcatt
     1021 cacataagtt gccgcatatg tttcatctac tataagatct ccaaaatcat acaaatcgtt
     1081 tttttctatg gggcaccagc tgtcggtacc ttccggcata aatcttctgg ccagatctct
     1141 tatcttcatt ggagcttcca ccgcaccttt gcccagcaga ctgcctccat catagggtat
     1201 gcccatcatg catacttttg cctg
//
LOCUS       DAONHK010000196         1175 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_118315_length_1175_cov_3.372321, whole genome shotgun
            sequence.
ACCESSION   DAONHK010000196 DAONHK010000000
VERSION     DAONHK010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1175
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118315_length_1175_cov_3.372321"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..>1175)
                     /locus_tag="PLM92_05930"
     CDS             complement(5..>1175)
                     /locus_tag="PLM92_05930"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPR24710.1"
                     /translation="PEEDKIFALNARAKKMAEEAGRDKVINATIGSLLDDDGKLVVLL
                     SVVKALKELEPMDFAEYAPIGGTPGFKKAVMKAAFGKHISSSFVEVVATPGGTGALRN
                     IVANYSKIGDKVLTSDWFWGPYKTITGELYRELCVYPMFDENNRFNRKGFSEELNKLA
                     KEQESVLILLNTPSHNPTGYSLDEDDWNAVSEAVKEAAEKARITLFVDAAYIDFAGDE
                     EEHRTFLPKLENLPSNVLPVIGYSFSKTFTLYGMRCGAMIAMAPSAEIAAEFKLVNEF
                     SARGTWSNSTRSGQVLVSKVYEDKDLLESVNKERAGYRQMLIKRGKTFSDAVIEAGLP
                     LVPFDSGFFSCIPVKDPAAVSKRLEEKGIFVVPLAKGIRYRWPLYRRKYAENFPG"
BASE COUNT          290 a          297 c          230 g          358 t
ORIGIN      
        1 gcgatcatcc cgggaagttt tctgcatact tcctccgata cagaggccac cgatatctta
       61 tgccctttgc caggggaacc acaaaaatgc ccttttcttc aagccttttg cttactgctg
      121 ccggatcttt tacaggtatg catgaaaaga atcccgaatc aaagggaaca aggggaagcc
      181 ccgcttcaat aactgcatcg ctgaaagttt ttcctctttt tatgagcatc tgtctgtatc
      241 cggctctttc tttattgaca ctttccagaa gatccttatc ttcatatact tttgaaacca
      301 gtacctgtcc gcttctggtg ctgtttgacc atgtgcctct tgcagaaaac tcattgacca
      361 gtttgaattc tgcagcgatc tccgctgaag gtgccatggc gatcattgct ccgcatctca
      421 ttccgtaaag agtaaaagtt ttagaaaaac tgtatccgat aacgggcaat acatttgatg
      481 gcagattttc aagttttgga agaaaagtcc tgtgttcctc ctcgtctccg gcgaaatcga
      541 tatacgccgc atctacaaaa agagttatcc ttgccttttc agcagcctct tttaccgctt
      601 ctgaaaccgc attccaatca tcttcgtcaa gagaataccc ggtgggattg tgagatggag
      661 tgttcaaaag gatcagaaca ctttcctgct cttttgccag cttgttgagc tcttcagaaa
      721 aaccctttct attgaatctg ttgttttcat caaacatggg atatacgcag agttccctgt
      781 aaagctctcc ggttattgtc ttatacggtc cccagaacca gtcggaagta aggactttgt
      841 ctccgatttt agaataatta gcaactatat ttctaagagc acccgttcct cccggtgtgg
      901 ccactacttc cacaaaggaa gaggatatgt gcttaccgaa agccgctttc atgacagctt
      961 ttttgaagcc cggtgttccg cctatcgggg catactctgc aaagtccata ggttcaagtt
     1021 cctttaatgc cttaactacc gataaaagca ccaccagttt gccgtcatca tcaagcagag
     1081 agccaatagt cgcattgatc actttgtccc ttcctgcctc ttcggccatt tttttcgctc
     1141 ttgcattaag ggcaaatatt ttatcctcct cgggt
//
LOCUS       DAONHK010000197         1081 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacillota bacterium isolate AMR_MDS_3793
            NODE_135653_length_1081_cov_3.217349, whole genome shotgun
            sequence.
ACCESSION   DAONHK010000197 DAONHK010000000
VERSION     DAONHK010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32865764
            Sequence Read Archive: SRR5007228
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacillota bacterium (wastewater metagenome)
  ORGANISM  Bacillota bacterium
            Bacteria; Bacillati; Bacillota.
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 01:06:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,189
            CDSs (total)                      :: 1,171
            Genes (coding)                    :: 1,154
            CDSs (with protein)               :: 1,154
            Genes (RNA)                       :: 18
            tRNAs                             :: 15
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 3 of 17
            Pseudo Genes (incomplete)         :: 13 of 17
            Pseudo Genes (internal stop)      :: 1 of 17
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1081
                     /organism="Bacillota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_135653_length_1081_cov_3.217349"
                     /isolate="AMR_MDS_3793"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:1879010"
                     /environmental_sample
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.28 N 114.17 E"
                     /collection_date="2015-03-06"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="PLM92_05935"
     CDS             <1..335
                     /locus_tag="PLM92_05935"
                     /inference="COORDINATES: protein motif:HMM:NF015559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HPR24711.1"
                     /translation="FCDNAGESIKLIKENVKACRAEDRSEIIHSDYRKALSKMEVQAD
                     IFFLDPPYKKDLWEKSMEMIAELGLLAPGGVIAAEHQRDKELPDEIACFKKTKERRYG
                     TVVLSLFV"
     gene            392..880
                     /gene="coaD"
                     /locus_tag="PLM92_05940"
     CDS             392..880
                     /gene="coaD"
                     /locus_tag="PLM92_05940"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HPR24712.1"
                     /translation="MRKALYAGSFDPLTCGHVDLIKRAAKLYDELVVGVISNPAKKPF
                     FTEEERKQMIKGAIDYIPNVTVDSFSGLLAEYVNANRFNVVIRGLRGSTDFEYEIQMA
                     QMNARLYKGKVETIFLMTDPSYSFISSSMAKEVYSLGGNIEGLVPESVLEVMNEKMKS
                     GR"
     gene            884..>1081
                     /locus_tag="PLM92_05945"
     CDS             884..>1081
                     /locus_tag="PLM92_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HPR24713.1"
                     /translation="MKVLELLDEIEEIVDTASGFPLTGKIMVDAGDLLEIVKEIRTEL
                     PDEIHQAQWIKNERDRILADAK"
BASE COUNT          356 a          198 c          272 g          255 t
ORIGIN      
        1 ttttctgcga taacgcagga gaaagcataa aactgataaa agaaaatgtg aaagcctgca
       61 gagccgaaga ccgttcggaa ataatccatt ccgattaccg caaggctctt tccaaaatgg
      121 aagttcaggc ggacattttt tttctggatc ccccctacaa aaaggacctc tgggagaaaa
      181 gcatggaaat gatagccgag cttggactcc tggctccggg aggcgtaata gcggcggaac
      241 atcaacggga caaagaattg ccggatgaaa tagcatgctt caaaaaaaca aaggagcgca
      301 ggtatggcac agttgtattg tcccttttcg tgtgatagaa tatttaaagt aatattcggc
      361 gaaatcctta aagattttac ggagggtgat aatgagaaaa gccttatatg caggatcttt
      421 tgatcctctg acctgcggac atgtggatct gataaaaagg gcagcaaaac tgtatgacga
      481 actggttgtg ggagtcataa gcaaccctgc caaaaaacct ttctttacag aggaagaaag
      541 aaaacagatg ataaaaggag ccattgacta tatccccaat gtcactgtgg attctttttc
      601 ggggcttttg gccgaatatg tgaacgccaa caggttcaat gtggtaataa gaggacttcg
      661 aggcagcact gattttgaat atgaaataca gatggcacaa atgaacgccc gcctttataa
      721 gggaaaggtc gagacgattt ttctcatgac cgatccaagt tattccttta taagctccag
      781 catggctaaa gaggtatatt cccttggtgg taatatagaa gggcttgttc ctgaaagcgt
      841 actggaagta atgaatgaga agatgaaatc cgggaggtag aaaatgaaag tacttgaact
      901 gttggatgaa atagaagaaa tcgttgatac cgcatcggga tttcctttga ccggaaaaat
      961 catggtggat gccggcgatc ttcttgaaat agtcaaagag ataaggacgg agctgccgga
     1021 cgagatacat caggcccagt ggatcaaaaa tgaaagagac aggatcctgg cagacgccaa
     1081 a
//
